8 results on '"Corti Monzón G"'
Search Results
2. Enrichment and key features of a robust and consistent indigenous marine-cognate microbial consortium growing on oily bilge wastewaters.
- Author
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Nisenbaum M, Corti-Monzón G, Villegas-Plazas M, Junca H, Mangani A, Patat ML, González JF, and Murialdo SE
- Subjects
- Biodegradation, Environmental, Hydrocarbons, Microbial Consortia, Petroleum, Wastewater
- Abstract
Oily bilge wastewater (OBW) is a hazardous hydrocarbon-waste generated by ships worldwide. In this research, we enriched, characterized and study the hydrocarbon biodegradation potential of a microbial consortium from the bilges of maritime ships. The consortium cZ presented a biodegradation efficiency of 66.65% for total petroleum hydrocarbons, 72.33% for aromatics and 97.76% removal of n-alkanes. This consortium showed the ability to grow in OBWs of diverse origin and concentration. A 67-fold increase in biomass was achieved using a Sequential Batch Reactor with OBW as the only carbon and energy source. The bacterial community composition of the enriched OBW bacterial consortium at the final stable stage was characterized by 16S amplicon Illumina sequencing showing that 25 out of 915 of the emerged predominant bacterial types detected summed up for 84% of total composition. Out of the 140 taxa detected, 13 alone accumulated 94.9% of the reads and were classified as Marinobacter, Alcanivorax, Parvibaculum, Flavobacteriaceae, Gammaproteobacteria PYR10d3, Novispirillum and Xanthomonadaceae among the most predominant, followed by Thalassospira, Shewanella, Rhodospirillaceae, Gammaprotobacteria, Rhodobacteriaceae and Achromobacter. The microbial community from OBW bioreactor enrichments is intrinsically diverse with clear selection of predominant types and remarkably exhibiting consistent and efficient biodegradation achieved without any nutrient or surfactant addition. Due to there is very little information available in the OBW biodegradation field, this work contributes to the body of knowledge surrounding the treatment improvement of this toxic waste and its potential application in wastewater management.
- Published
- 2020
- Full Text
- View/download PDF
3. Enrichment and characterization of a bilge microbial consortium with oil in water-emulsions breaking ability for oily wastewater treatment.
- Author
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Corti-Monzón G, Nisenbaum M, Villegas-Plazas M, Junca H, and Murialdo S
- Subjects
- Biodegradation, Environmental, Emulsions, RNA, Ribosomal, 16S, Shewanella, Microbial Consortia, Wastewater
- Abstract
Oily bilge wastewater is one of the main sources of hydrocarbons pollution in marine environments due to accidental or clandestine discharges. The main technical challenge for its effective treatment is the presence of stable oil-in-water (O/W) emulsions. In this work we are reporting an enriched microbial consortium from bilge wastewater with remarkable ability to demulsify oil in water emulsions. The consortium showed emulsion-breaking ratios up to 72.6% in the exponential growth phase, while the values range from 11.9 to 8.5% in stationary phase. A positive association was observed between demulsifying ability and microbial adhesion to hydrocarbons, as well as between cell concentration and demulsifying ability. Also, an interesting ability to demulsify under different temperatures, conditions of agitation, and bilge emulsions from different vessels was observed. The Bacterial and Archaeal composition was analyzed by 16S rRNA gene amplicon lllumina sequencing analyses, revealing an assemblage composed of bacterial types highly related to well characterized bacterial isolates and also to non-yet cultured bacterial types previously detected in marine and sediment samples. Hydrocarbonoclastic microbial types such as Marinobacter, Flavobacteriaceae, Alcanivorax and Gammaproteobacteria PYR10d3 were found in high relative abundance (27.0%-11.1%) and types of marine oligotrophs and surfactant degraders such as Thallasospira, Parvibaculum, Novospirillum, Shewanella algae, and Opitutae were in a group of middle predominance (1.7-3.5%). The microbial consortium reported has promising potential for the biological demulsification of bilge wastewater and other oily wastewaters.
- Published
- 2020
- Full Text
- View/download PDF
4. New Findings on Aromatic Compounds' Degradation and Their Metabolic Pathways, the Biosurfactant Production and Motility of the Halophilic Bacterium Halomonas sp. KHS3.
- Author
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Corti Monzón G, Nisenbaum M, Herrera Seitz MK, and Murialdo SE
- Subjects
- Argentina, Biodegradation, Environmental, DNA, Bacterial genetics, Halomonas enzymology, Salinity, Sodium Chloride analysis, Adipates metabolism, Benzoic Acid metabolism, Halomonas genetics, Halomonas metabolism, Parabens metabolism, Polycyclic Aromatic Hydrocarbons metabolism
- Abstract
The study of the aromatic compounds' degrading ability by halophilic bacteria became an interesting research topic, because of the increasing use of halophiles in bioremediation of saline habitats and effluents. In this work, we focused on the study of aromatic compounds' degradation potential of Halomonas sp. KHS3, a moderately halophilic bacterium isolated from hydrocarbon-contaminated seawater of the Mar del Plata harbour. We demonstrated that H. sp. KHS3 is able to grow using different monoaromatic (salicylic acid, benzoic acid, 4-hydroxybenzoic acid, phthalate) and polyaromatic (naphthalene, fluorene, and phenanthrene) substrates. The ability to degrade benzoic acid and 4-hydroxybenzoic acid was analytically corroborated, and Monod kinetic parameters and yield coefficients for degradation were estimated. Strategies that may enhance substrate bioavailability such as surfactant production and chemotactic responses toward aromatic compounds were confirmed. Genomic sequence analysis of this strain allowed us to identify several genes putatively related to the metabolism of aromatic compounds, being the catechol and protocatechuate branches of β-ketoadipate pathway completely represented. These features suggest that the broad-spectrum xenobiotic degrader H. sp. KHS3 could be employed as a useful biotechnological tool for the cleanup of aromatic compounds-polluted saline habitats or effluents.
- Published
- 2018
- Full Text
- View/download PDF
5. Effects of nitric oxide on sunflower seedlings: A balance between defense and development.
- Author
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Corti Monzón G, Regente M, Pinedo M, Lamattina L, and de la Canal L
- Subjects
- Helianthus drug effects, Helianthus microbiology, Hydroponics, Nitric Oxide Donors pharmacology, Plant Diseases microbiology, Seedlings drug effects, Seedlings microbiology, Verticillium drug effects, Verticillium physiology, Helianthus growth & development, Helianthus immunology, Nitric Oxide pharmacology, Plant Development drug effects, Seedlings growth & development, Seedlings immunology
- Abstract
Nitric oxide (NO) is a major plant signaling molecule that plays key roles during plant-pathogen interactions and plant development. Previous work showed the participation of NO in the development and lignin composition of sunflower roots. Thereby, we have hypothesized that NO applications could control the attack of the fungal pathogen Verticillium dahliae in sunflowers. Seedlings growing hydroponically were pretreated with NO donors and further inoculated with the fungus. Evaluation of disease symptoms showed that NO pretreatments could not reduce Verticillium wilt. Strikingly, NO donors appear to promote the fungal infection. These results indicate that NO applications were unable to protect sunflowers from Verticillium attack and highlight the role played by the fine tuning regulation of NO levels required to balance plant responses between development and defense.
- Published
- 2015
- Full Text
- View/download PDF
6. Nitric oxide is required for determining root architecture and lignin composition in sunflower. Supporting evidence from microarray analyses.
- Author
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Corti Monzón G, Pinedo M, Di Rienzo J, Novo-Uzal E, Pomar F, Lamattina L, and de la Canal L
- Subjects
- Free Radical Scavengers pharmacology, Gene Expression Profiling, Helianthus chemistry, Helianthus growth & development, Helianthus metabolism, Lignin analysis, Lignin chemistry, Nitric Oxide metabolism, Nitric Oxide Donors pharmacology, Oligonucleotide Array Sequence Analysis, Plant Proteins analysis, Plant Proteins genetics, Plant Proteins metabolism, Plant Roots chemistry, Plant Roots drug effects, Plant Roots metabolism, Helianthus drug effects, Nitric Oxide pharmacology
- Abstract
Nitric oxide (NO) is a signal molecule involved in several physiological processes in plants, including root development. Despite the importance of NO as a root growth regulator, the knowledge about the genes and metabolic pathways modulated by NO in this process is still limited. A constraint to unravel these pathways has been the use of exogenous applications of NO donors that may produce toxic effects. We have analyzed the role of NO in root architecture through the depletion of endogenous NO using the scavenger cPTIO. Sunflower seedlings growing in liquid medium supplemented with cPTIO showed unaltered primary root length while the number of lateral roots was deeply reduced; indicating that endogenous NO participates in determining root branching in sunflower. The transcriptional changes induced by NO depletion have been analyzed using a large-scale approach. A microarray analysis showed 330 genes regulated in the roots (p≤0.001) upon endogenous NO depletion. A general cPTIO-induced up-regulation of genes involved in the lignin biosynthetic pathway was observed. Even if no detectable changes in total lignin content could be detected, cell walls analyses revealed that the ratio G/S lignin increased in roots treated with cPTIO. This means that endogenous NO may control lignin composition in planta. Our results suggest that a fine tuning regulation of NO levels could be used by plants to regulate root architecture and lignin composition. The functional implications of these findings are discussed., (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
7. Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins.
- Author
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Regente M, Corti-Monzón G, Maldonado AM, Pinedo M, Jorrín J, and de la Canal L
- Subjects
- Amino Acid Sequence, Animals, Exosomes chemistry, Molecular Sequence Data, Seeds chemistry, Seeds ultrastructure, rab GTP-Binding Proteins metabolism, Biomarkers metabolism, Exosomes metabolism, Extracellular Fluid chemistry, Helianthus chemistry, Phospholipids chemistry, Plant Proteins metabolism
- Abstract
Based on the presence of phospholipids in the extracellular fluids (EFs) of sunflower seeds, we have hypothesized on the existence of vesicles in the apoplastic compartment of plants. Ultracentrifugation of sunflower EF allowed the isolation of particles of 50-200 nm with apparent membrane organization. A small GTPase Rab was putatively identified in this vesicular fraction. Since Rab proteins are involved in vesicular traffic and their presence in exosomes from animal fluids has been demonstrated, evidence presented here supports the existence of exosome-like vesicles in apoplastic fluids of sunflower. Their putative contribution to intercellular communication in plants is discussed.
- Published
- 2009
- Full Text
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8. Phospholipids are present in extracellular fluids of imbibing sunflower seeds and are modulated by hormonal treatments.
- Author
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Regente M, Corti Monzón G, and de la Canal L
- Subjects
- Abscisic Acid metabolism, Cyclopentanes metabolism, Oxylipins metabolism, Water metabolism, Extracellular Fluid metabolism, Helianthus metabolism, Phospholipids metabolism, Plant Growth Regulators metabolism, Seeds metabolism
- Abstract
Phospholipids are well known messengers involved in developmental and stress responses mediating intracellular signalling. It has been hypothesized that phospholipids exist which could participate in intercellular communication events through the apoplast of sunflower (Helianthus annuus) seeds. Here it is shown that extracellular washing fluids (EWFs) obtained from seeds imbibed for 2 h contain diverse phospholipids. Lipid profiling by electrospray ionization tandem mass spectrometry revealed that the EWFs have a particular composition, with phosphatidic acid (PA) and phosphatidylinositol (PI) being the major phospholipids. These profiles are clearly distinct from those of seed extract (SE), and comparative SDS-PAGE of EWF and SE, followed by intracellular and plasma membrane marker analyses, allowed a significant contamination of the EWF to be discarded. Treatment of the seeds with 100 microM jasmonic acid (JA) induces changes in the profile of EWF phospholipids, leading to a decrease in PI content, while the accumulation of phosphatidylinositol 4-phosphate (PI4P) and specific PA species is observed. On the other hand, the EWF from seeds subjected to 50 microM abscisic acid (ABA) treatment exhibit an increase in PA and phosphatidylglycerol levels. To our knowledge, this is the first report on the existence of phospholipids as extracellular components of seeds. Moreover, the modulation of PA, PI, and PI4P levels by hormonal treatments further suggests their contribution to intercellular communication in planta.
- Published
- 2008
- Full Text
- View/download PDF
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