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Your search keyword '"Cortez, David A."' showing total 463 results
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463 results on '"Cortez, David A."'

22. An intrinsic S/G₂ checkpoint enforced by ATR

Author

Saldivar, Joshua C., Hamperl, Stephan, Bocek, Michael J., Chung, Mingyu, Bass, Thomas E., Cisneros-Soberanis, Fernanda, Samejima, Kumiko, Xie, Linfeng, Paulson, James R., Earnshaw, William C., Cortez, David, Meyer, Tobias, and Cimprich, Karlene A.

Published
2018

31. Direct DNA Binding by Brca1

Author

Paull, Tanya T., Cortez, David, Bowers, Blair, Elledge, Stephen J., and Gellert, Martin

Published
2001

32. Structure of RADX and mechanism for regulation of RAD51 nucleofilaments.

Author

Balakrishnan, Swati, Adolph, Madison, Tsai, Miaw-Sheue, Akizuki, Tae, Gallagher, Kaitlyn, Cortez, David, and Chazin, Walter J.

Subjects
MOLECULAR structure, X chromosome, SINGLE-stranded DNA, DNA damage, FIBERS
Abstract

Replication fork reversal is a fundamental process required for resolution of encounters with DNA damage. A key step in the stabilization and eventual resolution of reversed forks is formation of RAD51 nucleoprotein filaments on exposed single strand DNA (ssDNA). To avoid genome instability, RAD51 filaments are tightly controlled by a variety of positive and negative regulators. RADX (RPA-related RAD51-antagonist on the X chromosome) is a recently discovered negative regulator that binds tightly to ssDNA, directly interacts with RAD51, and regulates replication fork reversal and stabilization in a context-dependent manner. Here, we present a structure-based investigation of RADX's mechanism of action. Mass photometry experiments showed that RADX forms multiple oligomeric states in a concentration-dependent manner, with a predominance of trimers in the presence of ssDNA. The structure of RADX, which has no structurally characterized orthologs, was determined ab initio by cryo-electron microscopy (cryo-EM) from maps in the 2 to 4 Å range. The structure reveals the molecular basis for RADX oligomerization and the coupled multi-valent binding of ssDNA binding. The interaction of RADX with RAD51 filaments was imaged by negative stain EM, which showed a RADX oligomer at the end of filaments. Based on these results, we propose a model in which RADX functions by capping and restricting the end of RAD51 filaments. [ABSTRACT FROM AUTHOR]

Published
2024
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36. Integrator facilitates RNAPII removal to prevent transcription-replication collisions and genome instability

Author

Bhowmick, Rahul, Mehta, Kavi P.M., Lerdrup, Mads, Cortez, David, Bhowmick, Rahul, Mehta, Kavi P.M., Lerdrup, Mads, and Cortez, David

Abstract

DNA replication preferentially initiates close to active transcription start sites (TSSs) in the human genome. Transcription proceeds discontinuously with an accumulation of RNA polymerase II (RNAPII) in a paused state near the TSS. Consequently, replication forks inevitably encounter paused RNAPII soon after replication initiates. Hence, dedicated machinery may be needed to remove RNAPII and facilitate unperturbed fork progression. In this study, we discovered that Integrator, a transcription termination machinery involved in the processing of RNAPII transcripts, interacts with the replicative helicase at active forks and promotes the removal of RNAPII from the path of the replication fork. Integrator-deficient cells have impaired replication fork progression and accumulate hallmarks of genome instability including chromosome breaks and micronuclei. The Integrator complex resolves co-directional transcription-replication conflicts to facilitate faithful DNA replication.

Published
2023

45. Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism

Author

Reynolds, John J, Bicknell, Louise S, Carroll, Paula, Higgs, Martin R, Shaheen, Ranad, Murray, Jennie E, Papadopoulos, Dimitrios K, Leitch, Andrea, Murina, Olga, Tarnauskaitė, Žygimantė, Wessel, Sarah R, Zlatanou, Anastasia, Vernet, Audrey, von Kriegsheim, Alex, Mottram, Rachel M A, Logan, Clare V, Bye, Hannah, Li, Yun, Brean, Alexander, Maddirevula, Sateesh, Challis, Rachel C, Skouloudaki, Kassiani, Almoisheer, Agaadir, Alsaif, Hessa S, Amar, Ariella, Prescott, Natalie J, Bober, Michael B, Duker, Angela, Faqeih, Eissa, Seidahmed, Mohammed Zain, Al Tala, Saeed, Alswaid, Abdulrahman, Ahmed, Saleem, Al-Aama, Jumana Yousuf, Altmüller, Janine, Al Balwi, Mohammed, Brady, Angela F, Chessa, Luciana, Cox, Helen, Fischetto, Rita, Heller, Raoul, Henderson, Bertram D, Hobson, Emma, Nürnberg, Peter, Percin, E Ferda, Peron, Angela, Spaccini, Luigina, Quigley, Alan J, Thakur, Seema, Wise, Carol A, Yoon, Grace, Alnemer, Maha, Tomancak, Pavel, Yigit, Gökhan, Taylor, A Malcolm R, Reijns, Martin A M, Simpson, Michael A, Cortez, David, Alkuraya, Fowzan S, Mathew, Christopher G, Jackson, Andrew P, and Stewart, Grant S

Published
2017
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48. Narrativas superpuestas, memorias en conflicto: repensar el centro histórico de Managua

Author

Rocha Cortez, David José

Subjects
Managua, memories, Centro histórico, Historical center, memorias
Abstract

The different elite groups of the country have used the historic center of Managua as a territory to print and superimpose their narratives, at the same time it has served as a scene of conflict between the dominant memories. The present work takes as a starting point the reading of this urban center as a document of memories. It stops at the analysis of some spaces that make up said center and places them in three temporalities: the Sandinista Popular Revolution and the rupture of the Somocista temporality, 1979 onwards; the end of the revolutionary period and the entrance to neoliberalism, 1990 onwards; the return of the Sandinista government as of 2007. You wonder what narratives the government elites projected on the historic center of the city of Managua? Who were and are its actors? What actions did they launch and how are such actions inscribed in the place, materializing and legitimizing their narratives about said space? Through what sociopolitical processes did other actors break with this narrative? Los diferentes grupos élites del país han utilizado el centro histórico de Managua como territorio para imprimir y superponer sus narrativas, al mismo tiempo, ha servido como escenario de conflicto entre las memorias dominantes. El presente trabajo toma como punto de partida la lectura de este centro urbano como documento de memorias. Se detiene en el análisis de algunos espacios que componen dicho centro y los sitúa en tres temporalidades: la Revolución Popular Sandinista y la ruptura de la temporalidad somocista, 1979 en adelante; el fin del período revolucionario y la entrada al neoliberalismo, 1990 en adelante; el regreso del gobierno sandinista a partir del 2007. Se pregunta ¿qué narrativas proyectaron las élites gubernamentales sobre el centro histórico de la ciudad de Managua? ¿Quiénes fueron y son sus actores? ¿Qué acciones pusieron en marcha y cómo se inscriben tales acciones en el lugar, materializando y legitimando sus narrativas sobre dicho espacio? ¿A través de qué procesos sociopolíticos otros actores rompieron con dicha narrativa?

Published
2022
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