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807 results on '"Corredig, Milena"'

38. Coupling in vitro food digestion with in vitro epithelial absorption; recommendations for biocompatibility.

Author

Kondrashina, Alina, Arranz, Elena, Cilla, Antonio, Faria, Miguel A., Santos-Hernández, Marta, Miralles, Beatriz, Hashemi, Negin, Rasmussen, Martin Krøyer, Young, Jette F., Barberá, Reyes, Mamone, Gianfranco, Tomás-Cobos, Lidia, Bastiaan-Net, Shanna, Corredig, Milena, and Giblin, Linda

Subjects
DIGESTIVE enzymes, BILE salts, EPITHELIAL cells, IN vitro studies, MONOMOLECULAR films
Abstract

As food transits the gastrointestinal tract, food structures are disrupted and nutrients are absorbed across the gut barrier. In the past decade, great efforts have focused on the creation of a consensus gastrointestinal digestion protocol (i.e., INFOGEST method) to mimic digestion in the upper gut. However, to better determine the fate of food components, it is also critical to mimic food absorption in vitro. This is usually performed by treating polarized epithelial cells (i.e., differentiated Caco-2 monolayers) with food digesta. This food digesta contains digestive enzymes and bile salts, and if following the INFOGEST protocol, at concentrations that although physiologically relevant are harmful to cells. The lack of a harmonized protocol on how to prepare the food digesta samples for downstream Caco-2 studies creates challenges in comparing inter laboratory results. This article aims to critically review the current detoxification practices, highlight potential routes and their limitations, and recommend common approaches to ensure food digesta is biocompatible with Caco-2 monolayers. Our ultimate aim is to agree a harmonized consensus protocol or framework for in vitro studies focused on the absorption of food components across the intestinal barrier. [ABSTRACT FROM AUTHOR]

Published
2024
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40. Ultrafiltration of Rapeseed Protein Concentrate: Effect of Pectinase Treatment on Membrane Fouling.

Author

Alpiger, Simone Bleibach, Solet, Chloé, Dang, Tem Thi, and Corredig, Milena

Subjects
MEMBRANE separation, MOLECULAR weights, RAPESEED, PECTIC enzymes, PSEUDOPOTENTIAL method
Abstract

Membrane filtration technologies have shown great potential as a gentle and effective method for concentrating and fractionating proteins for food applications. However, the application of this technology to plant-derived protein streams is in its infancy. In this study, an aqueous rapeseed protein concentrate was obtained with wet milling, and its performance during ultrafiltration with two distinct molecular weight cut-offs (10 and 100 kDa) was tested. All rapeseed proteins were retained during filtration. The addition of pectinase during extraction prior to filtration caused important structural modifications to the extract, resulting in increased permeate fluxes, increased carbohydrate permeation and a reduction in irreversible fouling. Lager pore sizes led to more pronounced fouling. FTIR analysis of the spent membranes showed that proteins and lipids are causing irreversible fouling. [ABSTRACT FROM AUTHOR]

Published
2024
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43. Sodium caseinate stabilized emulsions as a delivery system for epigallocatechin-gallate: Bioaccessibility, anti-proliferative activity and intestinal absorption

Author

Sabouri, Somayeh, Arranz Gutiérrez, Elena María, Corredig, Milena, Guri, Anilda, Sabouri, Somayeh, Arranz Gutiérrez, Elena María, Corredig, Milena, and Guri, Anilda

Abstract

The objective of this study was to investigate the effect of complexation of epigallocatechin-gallate (EGCG) to a caseinate-stabilized oil-water interface on the bioefficacy of EGCG. The bioaccessibility, anti-proliferative activity, and intestinal uptake of EGCG are reported. Higher amount of EGCG was present in digested emulsions compared to their equivalent solutions after digestion. The incorporation of EGCG in sodium caseinate emulsions improved its bioaccesibility and this was confirmed by cytotoxicity assays on Caco-2 cells. Intestinal absorption of EGCG was also studied with in vitro transport experiments. It was difficult to obtain a quantitative measurement of the EGCG in the basolateral fraction, but the fractions showed a clear anti-proliferative activity. These results would suggest the use of a cytotoxicity assay on cell cultures to estimate the extent of intestinal absorption of the bioactive catechin EGCG. The findings demonstrated that sodium caseinate-stabilized emulsions can be used as a platform for delivery of EGCG., Natural Sciences and Engineering Research Council of Canada, EU COST Action INFOGEST, Depto. de Nutrición y Ciencia de los Alimentos, Fac. de Farmacia, TRUE, pub

Published
2024

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