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Your search keyword '"Corrado, Alessio"' showing total 9 results
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9 results on '"Corrado, Alessio"'

1. Innovative Reversed-Phase Chromatography Platform Approach for the Fast and Accurate Characterization of Membrane Vesicles’ Protein Patterns

Author

Nompari, Luca, primary, Coccone, Salvatore Sanna, additional, Sardone, Gian Luca, additional, Corrado, Alessio, additional, Berti, Stefania, additional, Biagini, Massimiliano, additional, Rovini, Michele, additional, Magagnoli, Claudia, additional, Cianetti, Simona, additional, Orlandini, Serena, additional, Furlanetto, Sandra, additional, and De Ricco, Riccardo, additional

Published
2024
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3. Retaining the structural integrity of disulfide bonds in diphtheria toxoid carrier protein is crucial for the effectiveness of glycoconjugate vaccine candidates

Author

Carboni, Filippo, Kitowski, Annabel, Sorieul, Charlotte, Veggi, Daniele, Marques, Marta C, Oldrini, Davide, Balducci, Evita, Brogioni, Barbara, Del Bino, Linda, Corrado, Alessio, Angiolini, Francesca, Dello Iacono, Lucia, Margarit, Immaculada, Romano, Maria Rosaria, Bernardes, Gonçalo JL, Adamo, Roberto, Kitowski, Annabel [0000-0002-8001-5928], Sorieul, Charlotte [0000-0003-1999-6007], Bernardes, Gonçalo JL [0000-0001-6594-8917], Adamo, Roberto [0000-0001-5228-6088], and Apollo - University of Cambridge Repository

Subjects
34 Chemical Sciences, Prevention, 1.1 Normal biological development and functioning, 3 Good Health and Well Being, General Chemistry, FOS: Health sciences, Vaccine Related, Infectious Diseases, Emerging Infectious Diseases, 3.4 Vaccines, 1 Underpinning research, Immunization, Biotechnology, 3 Prevention of disease and conditions, and promotion of well-being
Abstract

The introduction of glycoconjugate vaccines marks an important point in the fight against various infectious diseases. The covalent conjugation of relevant polysaccharide antigens to immunogenic carrier proteins enables the induction of a long-lasting and robust IgG antibody response, which is not observed for pure polysaccharide vaccines. Although there has been remarkable progress in the development of glycoconjugate vaccines, many crucial parameters remain poorly understood. In particular, the influence of the conjugation site and strategy on the immunogenic properties of the final glycoconjugate vaccine is the focus of intense research. Here, we present a comparison of two cysteine selective conjugation strategies, elucidating the impact of both modifications on the structural integrity of the carrier protein, as well as on the immunogenic properties of the resulting glycoconjugate vaccine candidates. Our work suggests that conjugation chemistries impairing structurally relevant elements of the protein carrier, such as disulfide bonds, can have a dramatic effect on protein immunogenicity.

Published
2022
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7. Evaluation of Immune Responses to Group B Streptococcus Type III Oligosaccharides Containing a Minimal Protective Epitope.

Author

Carboni, Filippo, Angiolini, Francesca, Fabbrini, Monica, Brogioni, Barbara, Corrado, Alessio, Berti, Francesco, Adamo, Roberto, and Margarit, Immaculada

Subjects
STREPTOCOCCUS agalactiae, OLIGOSACCHARIDES, IMMUNE response, CARRIER proteins, MONOCLONAL antibodies, STREPTOCOCCAL disease prevention, RESEARCH, ANIMAL experimentation, RESEARCH methodology, EVALUATION research, MEDICAL cooperation, STREPTOCOCCUS, COMPARATIVE studies, MOLECULAR structure, ANTIGENS, BACTERIAL vaccines, MICE
Abstract

Recent structural studies demonstrated that the epitope recognized by a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide was comprised within 2 of the repeating units that constitute the full-length native structure. In the current study, we took advantage of this discovery to design a novel vaccine based on multivalent presentation of the identified minimal epitope on a carrier protein. We show that highly glycosylated short oligosaccharide conjugates elicit functional immune responses comparable to those of the full-length native polysaccharide. The obtained results pave the way to the design of well-defined glycoconjugate vaccines based on short synthetic oligosaccharides. [ABSTRACT FROM AUTHOR]

Published
2020
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8. The Conformation of the Mannopyranosyl Phosphate Repeating Unit of the Capsular Polysaccharide of Neisseria meningitidis Serogroup A and Its Carba‐Mimetic

Author

Calloni, Ilaria, primary, Unione, Luca, additional, Jiménez‐Osés, Gonzalo, additional, Corzana, Francisco, additional, Del Bino, Linda, additional, Corrado, Alessio, additional, Pitirollo, Olimpia, additional, Colombo, Cinzia, additional, Lay, Luigi, additional, Adamo, Roberto, additional, and Jiménez‐Barbero, Jesús, additional

Published
2018
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