Author: "Corneau, Aurélien" - Searchworks@Jio Institute Digital Library Search Results

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140 results on '"Corneau, Aurélien"'

1. VEXAS syndrome is characterized by inflammasome activation and monocyte dysregulation

Author: Kosmider, Olivier, Possémé, Céline, Templé, Marie, Corneau, Aurélien, Carbone, Francesco, Duroyon, Eugénie, Breillat, Paul, Chirayath, Twinu-Wilson, Oules, Bénédicte, Sohier, Pierre, Luka, Marine, Gobeaux, Camille, Lazaro, Estibaliz, Outh, Roderau, Le Guenno, Guillaume, Lifermann, François, Berleur, Marie, Le Mene, Melchior, Friedrich, Chloé, Lenormand, Cédric, Weitten, Thierry, Guillotin, Vivien, Burroni, Barbara, Boussier, Jeremy, Willems, Lise, Aractingi, Selim, Dionet, Léa, Tharaux, Pierre-Louis, Vergier, Béatrice, Raynaud, Pierre, Ea, Hang-Korng, Ménager, Mickael, Duffy, Darragh, and Terrier, Benjamin
Published: 2024
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2. NBEAL2 deficiency in humans leads to low CTLA-4 expression in activated conventional T cells

Author: Delage, Laure, Carbone, Francesco, Riller, Quentin, Zachayus, Jean-Luc, Kerbellec, Erwan, Buzy, Armelle, Stolzenberg, Marie-Claude, Luka, Marine, de Cevins, Camille, Kalouche, Georges, Favier, Rémi, Michel, Alizée, Meynier, Sonia, Corneau, Aurélien, Evrard, Caroline, Neveux, Nathalie, Roudières, Sébastien, Pérot, Brieuc P., Fusaro, Mathieu, Lenoir, Christelle, Pellé, Olivier, Parisot, Mélanie, Bras, Marc, Héritier, Sébastien, Leverger, Guy, Korganow, Anne-Sophie, Picard, Capucine, Latour, Sylvain, Collet, Bénédicte, Fischer, Alain, Neven, Bénédicte, Magérus, Aude, Ménager, Mickaël, Pasquier, Benoit, and Rieux-Laucat, Frédéric
Published: 2023
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3. Successful treatment of JAK1-associated inflammatory disease

Author: Fayand, Antoine, Hentgen, Véronique, Posseme, Céline, Lacout, Carole, Picard, Capucine, Moguelet, Philippe, Cescato, Margaux, Sbeih, Nabiha, Moreau, Thomas R.J., Zhu, Yixiang Y.J., Charuel, Jean-Luc, Corneau, Aurélien, Deibener-Kaminsky, Joelle, Dupuy, Stéphanie, Fusaro, Mathieu, Hoareau, Benedicte, Hovnanian, Alain, Langlois, Vincent, Le Corre, Laurent, Maciel, Thiago T., Miskinyte, Snaigune, Miyara, Makoto, Moulinet, Thomas, Perret, Magali, Schuhmacher, Marie Hélène, Rignault-Bricard, Rachel, Viel, Sébastien, Vinit, Angélique, Soria, Angèle, Duffy, Darragh, Launay, Jean-Marie, Callebert, Jacques, Herbeuval, Jean Philippe, Rodero, Mathieu P., and Georgin-Lavialle, Sophie
Published: 2023
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4. Azithromycin promotes relapse by disrupting immune and metabolic networks after allogeneic stem cell transplantation

Author: Vallet, Nicolas, Le Grand, Sophie, Bondeelle, Louise, Hoareau, Bénédicte, Corneau, Aurélien, Bouteiller, Delphine, Tournier, Simon, Derivry, Lucille, Bohineust, Armelle, Tourret, Marie, Gibert, Delphine, Mayeur, Ethan, Itzykson, Raphael, Pacchiardi, Kim, Ingram, Brian, Cassonnet, Stéphane, Lepage, Patricia, Peffault de Latour, Régis, Socié, Gérard, Bergeron, Anne, and Michonneau, David
Published: 2022
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5. Immune landscape after allo-HSCT: TIGIT- and CD161-expressing CD4 T cells are associated with subsequent leukemia relapse

Author: Gournay, Viviane, Vallet, Nicolas, Peux, Vivien, Vera, Kristi, Bordenave, Jennifer, Lambert, Marion, Corneau, Aurélien, Michonneau, David, Peffault de Latour, Régis, Caillat-Zucman, Sophie, Socié, Gérard, and Chevalier, Mathieu F.
Published: 2022
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6. 94 Dominant negative IKKα and immunodeficiency with immune dysregulation

Author: Riller, Quentin, primary, Sorin, Boris, additional, Courteille, Charline, additional, Voyer, Tom Le, additional, Pellé, Olivier, additional, Stolzenberg, Marie-Claude, additional, Jeanpierre, Marie, additional, Becquard, Thomas, additional, Boussard, Charlotte, additional, Michel, Victor, additional, Rodrigo-Riestra, Maria, additional, Picard, Capucine, additional, Corneau, Aurélien, additional, Meyts, Isabelle, additional, Baud, Veronique, additional, Fischer, Alain, additional, Puel, Anne, additional, Casanova, Jean-Laurent, additional, Boulanger, Cécile, additional, Neven, Bénédicte, additional, and Rieux-Laucat, Frédéric, additional
Published: 2024
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7. A monocyte/dendritic cell molecular signature of SARS-CoV-2-related multisystem inflammatory syndrome in children with severe myocarditis

Author: de Cevins, Camille, Luka, Marine, Smith, Nikaïa, Meynier, Sonia, Magérus, Aude, Carbone, Francesco, García-Paredes, Víctor, Barnabei, Laura, Batignes, Maxime, Boullé, Alexandre, Stolzenberg, Marie-Claude, Pérot, Brieuc P., Charbit, Bruno, Fali, Tinhinane, Pirabakaran, Vithura, Sorin, Boris, Riller, Quentin, Abdessalem, Ghaith, Beretta, Maxime, Grzelak, Ludivine, Goncalves, Pedro, Di Santo, James P., Mouquet, Hugo, Schwartz, Olivier, Zarhrate, Mohammed, Parisot, Mélanie, Bole-Feysot, Christine, Masson, Cécile, Cagnard, Nicolas, Corneau, Aurélien, Brunaud, Camille, Zhang, Shen-Ying, Casanova, Jean-Laurent, Bader-Meunier, Brigitte, Haroche, Julien, Melki, Isabelle, Lorrot, Mathie, Oualha, Mehdi, Moulin, Florence, Bonnet, Damien, Belhadjer, Zahra, Leruez, Marianne, Allali, Slimane, Gras-Leguen, Christèle, de Pontual, Loïc, Fischer, Alain, Duffy, Darragh, Rieux-Laucat, Fredéric, Toubiana, Julie, and Ménager, Mickaël M.
Published: 2021
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8. Mass cytometry reveals atypical immune profile notably impaired maturation of memory CD4 T with Gb3‐related CD27 expression in CD4 T cells in Fabry disease.

Author: Mauhin, Wladimir, Dzangue‐Tchoupou, Gaelle, Amelin, Damien, Corneau, Aurélien, Lamari, Foudil, Allenbach, Yves, Dussol, Bertrand, Leguy‐Seguin, Vanessa, D'Halluin, Pauline, Matignon, Marie, Maillot, François, Ly, Kim‐Heang, Besson, Gérard, Willems, Marjolaine, Labombarda, Fabien, Masseau, Agathe, Lavigne, Christian, Lacombe, Didier, Maillard, Hélène, and Lidove, Olivier
Abstract: Fabry disease (FD) is an X‐linked disease characterized by an accumulation of glycosphingolipids, notably of globotriaosylceramide (Gb3) and globotriaosylsphingosine (lysoGb3) leading to renal failure, cardiomyopathy, and cerebral strokes. Inflammatory processes are involved in the pathophysiology. We investigated the immunological phenotype of peripheral blood mononuclear cells in Fabry patients depending on the clinical phenotype, treatment, Gb3, and lysoGb3 levels and the presence of anti‐drug antibodies (ADA). Leucocytes from 41 male patients and 20 controls were analyzed with mass cytometry using both unsupervised and supervised algorithms. FD patients had an increased expression of CD27 and CD28 in memory CD45‐ and CD45 + CCR7‐CD4 T cells (respectively p < 0.014 and p < 0.02). Percentage of CD45RA‐CCR7‐CD27 + CD28+ cells in CD4 T cells was correlated with plasma lysoGb3 (r = 0.60; p = 0.0036) and phenotype (p < 0.003). The correlation between Gb3 and CD27 in CD4 T cells almost reached significance (r = 0.33; p = 0.058). There was no immune profile associated with the presence of ADA. Treatment with agalsidase beta was associated with an increased proportion of Natural Killer cells. These findings provide valuable insights for understanding FD, linking Gb3 accumulation to inflammation, and proposing new prognostic biomarkers. [ABSTRACT FROM AUTHOR]
Published: 2024
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9. Phenotyping of tumor infiltrating immune cells using mass-cytometry (CyTOF)

Author: Maby, Pauline, primary, Corneau, Aurélien, additional, and Galon, Jérôme, additional
Published: 2020
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10. 94 Compound heterozygous mutations in the kinase domain of IKKα lead to immunodeficiency and immune dysregulation

Author: Riller, Quentin, Sorin, Boris, Courteille, Charline, Voyer, Tom Le, Pellé, Olivier, Stolzenberg, Marie-Claude, Jeanpierre, Marie, Becquard, Thomas, Boussard, Charlotte, Michel, Victor, Rodrigo-Riestra, Maria, Picard, Capucine, Corneau, Aurélien, Meyts, Isabelle, Baud, Veronique, Fischer, Alain, Puel, Anne, Casanova, Jean-Laurent, Boulanger, Cécile, Neven, Bénédicte, and Rieux-Laucat, Frédéric
Published: 2024
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11. Single-cell mass cytometry on peripheral cells in Myasthenia Gravis identifies dysregulation of innate immune cells

Author: Verdier, Julien, primary, Fayet, Odessa-Maud, additional, Hemery, Edouard, additional, Truffault, Frédérique, additional, Pinzón, Natalia, additional, Demeret, Sophie, additional, Behin, Anthony, additional, Fadel, Elie, additional, Guihaire, Julien, additional, Corneau, Aurélien, additional, Blanc, Catherine, additional, Berrih-Aknin, Sonia, additional, and Le Panse, Rozen, additional
Published: 2023
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12. VEXAS syndrome is characterized by blood and tissues inflammasome pathway activation and monocyte dysregulation

Author: Kosmider, Olivier, primary, Possémé, Céline, additional, Templé, Marie, additional, Corneau, Aurélien, additional, Carbone, Francesco, additional, Duroyon, Eugénie, additional, Chirayath, Twinu-Wilson, additional, Luka, Marine, additional, Gobeaux, Camille, additional, Lazaro, Estibaliz, additional, Outh, Roderau, additional, Le Guenno, Guillaume, additional, Lifermann, François, additional, Berleur, Marie, additional, Friedrich, Chloé, additional, Lenormand, Cédric, additional, Weitten, Thierry, additional, Guillotin, Vivien, additional, Burroni, Barbara, additional, Sohier, Pierre, additional, Boussier, Jay, additional, Willems, Lise, additional, Aractingi, Selim, additional, Dionet, Léa, additional, Tharaux, Pierre-Louis, additional, Vergier, Béatrice, additional, Raynaud, Pierre, additional, Ea, Hang-Korng, additional, Ménager, Mickael, additional, Duffy, Darragh, additional, and Terrier, Benjamin, additional
Published: 2022
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13. Characterization of circulating immune cells in Myasthenia Gravis by mass cytometry revealed dysregulation on innate immune cells

Author: Verdier, Julien, Hemery, Edouard, Fayet, Odessa-Maud, Truffault, Frédérique, Corneau, Aurélien, Blanc, Catherine, Behin, Anthony, Demeret, Sophie, Berrih-Aknin, Sonia, Le Panse, Rozen, Centre de recherche en Myologie – U974 SU-INSERM, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut de Myologie, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Cytométrie Pitié-Salpêtrière (PASS-CYPS), Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé (PASS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Plateforme cytométrie en flux, Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR113, Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), CHU Pitié-Salpêtrière [AP-HP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Subjects: [SDV.IMM]Life Sciences [q-bio]/Immunology
Abstract: International audience; Autoimmune Myasthenia Gravis (MG) is characterized by invalidating muscle weaknesses due especially to anti-acetylcholine receptor autoantibodies. As for other autoimmune diseases, immune dysregulations are well known for adaptive immune cells, such as B and T cells. However, to further gain insight into immune dysregulation underlying MG, we performed an in-depth analysis on peripheral mononuclear blood cells using mass cytometry.Cells from 24 AChR+ MG patients and 16 age- and sex-matched controls were stained with 37 antibodies and acquired on a Helios™ mass cytometer. Using both unsupervised and supervised approaches, we identified several circulating cell subpopulations whom expression was affected in MG, and that had not been previously associated with this disease. MG was associated with a reduction of circulating monocytes, for all subpopulations: classical (CD14++CD16-), intermediate (CD14+/++CD16+) and non-classical (CD14low CD16+) monocytes. In contrast, an increase in innate lymphoid cells 2 (ILC-2: CD161+CRTH2+) and of gδ T17 cells (gδ+CD27-) was observed in MG patients. This increase in gδ T17 cells was not detected in periphery by classical flow cytometry that might be less discriminative than mass cytometry but was clearly detected in thymic cells of MG patients. These analyses have unraveled unexpected dysregulations on innate immune cells and further investigations are ongoing to better understand their implication in MG. Innate immunity is crucial for host defense, but its improper activation could also be involved in autoimmunity.
Published: 2022

14. A negative feedback loop between fibroadipogenic progenitors and muscle fibres involving endothelin promotes human muscle fibrosis

Author: Bensalah, Mona, primary, Muraine, Laura, additional, Boulinguiez, Alexis, additional, Giordani, Lorenzo, additional, Albert, Victorine, additional, Ythier, Victor, additional, Dhiab, Jamila, additional, Oliver, Alison, additional, Hanique, Valentine, additional, Gidaro, Teresa, additional, Perié, Sophie, additional, Lacau St‐Guily, Jean, additional, Corneau, Aurélien, additional, Butler‐Browne, Gillian, additional, Bigot, Anne, additional, Mouly, Vincent, additional, Negroni, Elisa, additional, and Trollet, Capucine, additional
Published: 2022
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15. Two New Neutrophil Subsets Define a Discriminating Sepsis Signature

Author: Borok, Matthew, Didier, Nathalie, Gattazzo, Francesca, Ozturk, Teoman, Corneau, Aurélien, Rouard, Helene, Relaix, Frederic, Meghraoui-Kheddar, Aïda, Chousterman, Benjamin, Guillou, Noëlline, Barone, Sierra, Granjeaud, Samuel, Vallet, Helene, Guessous, Karim, de Roquetaillade, Charles, Boissonnas, Alexandre, Irish, Jonathan, Combadière, Christophe, Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Marqueurs cardiovasculaires en situation de stress (MASCOT (UMR_S_942 / U942)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Université Sorbonne Paris Nord, Hôpital Lariboisière-Fernand-Widal [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Vanderbilt University Medical Center [Nashville], Vanderbilt University [Nashville], Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU), Service d'Unité de gériatrie aigüe [CHU Saint-Antoine], CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Département d’Anesthésie-Réanimation-SMUR [Hôpital Lariboisière], Hôpitaux Universitaire Saint-Louis, Lariboisière, Fernand-Widal, Cytométrie Pitié-Salpêtrière (PASS-CYPS), Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé (PASS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), BRUNEL, Nadège, and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Subjects: PD-L1, Pulmonary and Respiratory Medicine, medicine.medical_specialty, diagnosis, Neutrophils, [SDV]Life Sciences [q-bio], Interleukin-3 Receptor alpha Subunit, 030204 cardiovascular system & hematology, Critical Care and Intensive Care Medicine, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Sensitivity and Specificity, Severity of Illness Index, B7-H1 Antigen, Diagnosis, Differential, Sepsis, 03 medical and health sciences, 0302 clinical medicine, Immune system, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Clinical Decision Rules, Intensive care, medicine, Humans, Longitudinal Studies, Clinical care, Intensive care medicine, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, 030304 developmental biology, Cause of death, 0303 health sciences, biology, business.industry, Receptors, IgG, Flow Cytometry, medicine.disease, CD123, Case-Control Studies, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Linear Models, biology.protein, [SDV.IMM]Life Sciences [q-bio]/Immunology, Interleukin-3 receptor, business, Biomarkers
Abstract: International audience; Background: Skeletal muscle is one of the only mammalian tissues capable of rapid and efficient regeneration after trauma or in pathological conditions. Skeletal muscle regeneration is driven by the muscle satellite cells, the stem cell population in interaction with their niche. Upon injury, muscle fibers undergo necrosis and muscle stem cells activate, proliferate and fuse to form new myofibers. In addition to myogenic cell populations, interaction with other cell types such as inflammatory cells, mesenchymal (fibroadipogenic progenitors—FAPs, pericytes) and vascular (endothelial) lineages are important for efficient muscle repair. While the role of the distinct populations involved in skeletal muscle regeneration is well characterized, the quantitative changes in the muscle stem cell and niche during the regeneration process remain poorly characterized. Methods: We have used mass cytometry to follow the main muscle cell types (muscle stem cells, vascular, mesenchymal and immune cell lineages) during early activation and over the course of muscle regeneration at D0, D2, D5 and D7 compared with uninjured muscles. Results: Early activation induces a number of rapid changes in the proteome of multiple cell types. Following the induction of damage, we observe a drastic loss of myogenic, vascular and mesenchymal cell lineages while immune cells invade the damaged tissue to clear debris and promote muscle repair. Immune cells constitute up to 80% of the mononuclear cells 5 days post-injury. We show that muscle stem cells are quickly activated in order to form new myofibers and reconstitute the quiescent muscle stem cell pool. In addition, our study provides a quantitative analysis of the various myogenic populations during muscle repair. Conclusions: We have developed a mass cytometry panel to investigate the dynamic nature of muscle regeneration at a single-cell level. Using our panel, we have identified early changes in the proteome of stressed satellite and niche cells. We have also quantified changes in the major cell types of skeletal muscle during regeneration and analyzed myogenic transcription factor expression in satellite cells throughout this process. Our results highlight the progressive dynamic shifts in cell populations and the distinct states of muscle stem cells adopted during skeletal muscle regeneration. Our findings give a deeper understanding of the cellular and molecular aspects of muscle regeneration.
Published: 2022

16. Naive and memory CD4+ T cell subsets can contribute to the generation of human Tfh cells

Author: Jeger-Madiot, Raphaël, Vaineau, Romain, Heredia, Maud, Tchitchek, Nicolas, Bertrand, Lisa, Pereira, Mathias, Konza, Océane, Gouritin, Bruno, Hoareau-Coudert, Bénédicte, Corneau, Aurélien, Blanc, Catherine, Savier, Eric, Buffet, Pierre, Six, Adrien, Klatzmann, David, Moris, Arnaud, Graff-Dubois, Stéphanie, Centre d'Immunologie et de Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Immunologie - Immunopathologie - Immunothérapie [CHU Pitié Salpêtrière] (I3), CHU Charles Foix [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Cytométrie Pitié-Salpêtrière (PASS-CYPS), Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé (PASS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Service d'Imagerie et de Cytométrie [CHU Saint-Antoine] (UMS LUMIC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Service de Chirurgie Digestive, Hépato-Bilio-pancréatique et Transplantation Hépatique [CHU Pitié-Salpétrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Biologie Intégrée du Globule Rouge (BIGR (UMR_S_1134 / U1134)), Institut National de la Transfusion Sanguine [Paris] (INTS)-Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Université des Antilles (UA)-Université de Paris (UP), Departement Hospitalo- Universitaire - Inflammation, Immunopathologie, Biothérapie [Paris] (DHU - I2B), Sorbonne Université (SU)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Trousseau [APHP], CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Centre d'Immunologie et des Maladies Infectieuses (CIMI), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut National de la Transfusion Sanguine [Paris] (INTS)-Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Université des Antilles (UA)-Université Paris Cité (UPCité), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-CHU Saint-Antoine [AP-HP], and Tchitchek, Nicolas
Subjects: [SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [SDV.IMM]Life Sciences [q-bio]/Immunology
Abstract: International audience; CD4 + T follicular helper cells (Tfh) promote B cell maturation and antibody production in secondary lymphoid organs. By using an innovative culture system based on splenocyte stimulation, we studied the dynamics of naive and memory CD4 + T cells during the generation of a Tfh cell response. We found that both naive and memory CD4 + T cells can acquire phenotypic and functional features of Tfh cells. Moreover, we show here that the transition of memory as well as naive CD4 + T cells into the Tfh cell profile is supported by the expression of pro-Tfh genes, including transcription factors known to orchestrate Tfh cell development. Using this culture system, we provide pieces of evidence that HIV infection differentially alters these newly identified pathways of Tfh cell generation. Such diversity in pathways of Tfh cell generation offers a new framework for the understanding of Tfh cell responses in physiological and pathological contexts.
Published: 2021

17. Operational tolerance after hematopoietic stem cell transplantation is characterized by distinct transcriptional, phenotypic, and metabolic signatures

Author: Dubouchet, Laetitia, primary, Todorov, Helena, additional, Seurinck, Ruth, additional, Vallet, Nicolas, additional, Van Gassen, Sofie, additional, Corneau, Aurélien, additional, Blanc, Catherine, additional, Zouali, Habib, additional, Boland, Anne, additional, Deleuze, Jean-François, additional, Ingram, Brian, additional, de Latour, Regis Peffault, additional, Saeys, Yvan, additional, Socié, Gérard, additional, and Michonneau, David, additional
Published: 2022
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18. Two New Neutrophil Subsets Define a Discriminating Sepsis Signature

Author: Meghraoui-Kheddar, Aïda, primary, Chousterman, Benjamin G., additional, Guillou, Noëlline, additional, Barone, Sierra M., additional, Granjeaud, Samuel, additional, Vallet, Helene, additional, Corneau, Aurélien, additional, Guessous, Karim, additional, de Roquetaillade, Charles, additional, Boissonnas, Alexandre, additional, Irish, Jonathan M., additional, and Combadière, Christophe, additional
Published: 2022
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19. Naive and memory CD4+ T cell subsets can contribute to the generation of human Tfh cells

Author: Jeger-Madiot, Raphaël, primary, Vaineau, Romain, additional, Heredia, Maud, additional, Tchitchek, Nicolas, additional, Bertrand, Lisa, additional, Pereira, Mathias, additional, Konza, Océane, additional, Gouritin, Bruno, additional, Hoareau-Coudert, Bénédicte, additional, Corneau, Aurélien, additional, Blanc, Catherine, additional, Savier, Eric, additional, Buffet, Pierre, additional, Six, Adrien, additional, Klatzmann, David, additional, Moris, Arnaud, additional, and Graff-Dubois, Stéphanie, additional
Published: 2022
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20. Nucleocapsid-specific and PD-L1+CXCR3+ CD8 polyfunctional T-cell abundances are associated with survival of critical SARS-CoV2-infected patients

Author: Adam, Lucille, Rosenbaum, Pierre, Quentric, Paul, Parizot, Christophe, Bonduelle, Olivia, Guillou, Noëlline, Corneau, Aurélien, Dorgham, Karim, Miyara, Makoto, Luyt, Charles-Edouard, Guihot, Amélie, Gorochov, Guy, Combadière, Christophe, Combadière, Behazine, Centre d'Immunologie et de Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Cytométrie Pitié-Salpêtrière (PASS-CYPS), Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé (PASS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Gestionnaire, HAL Sorbonne Université 5, Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Subjects: [SDV.IMM] Life Sciences [q-bio]/Immunology, [SDV.IMM]Life Sciences [q-bio]/Immunology
Abstract: International audience; Rationale. The importance of the adaptative T cell response in the control and resolution of viral infection has been well-established. However, the nature of T cell-mediated viral control mechanisms in life-threatening stages of COVID-19 has yet to be determined.Objective. The aim of the present study was to determine the function and phenotype of T cell populations associated with survival or death of COVID-19 patients under intensive care as a result of phenotypic and functional profiling by mass cytometry.Findings. Increased frequencies of circulating, polyfunctional, CD4+CXCR5+HLA-DR+ stem cell memory T cells (TSCM) and decreased proportions of Granzyme-B and Perforin-expressing effector memory T cells (TEM) were detected in recovered and deceased patients, respectively. The higher abundance of polyfunctional CD8+PD-L1+CXCR3+ T effector cells, CXCR5+HLA-DR+ TSCM, as well as anti-nucleocapsid (NC) cytokine-producing T cells permitted to differentiate between recovered and deceased patients. The results from a principal component analysis showed an imbalance in the T cell compartment allowed for the separation of recovered and deceased patients. The paucity of circulating CD8+PD-L1+CXCR3+ Teff-cells and NC-specific CD8+ T-cells accurately forecasts fatal disease outcome.Conclusion. This study provides insight into the nature of the T cell populations involved in the control of COVID-19 and therefor might impact T cell-based vaccine designs for this infectious disease.
Published: 2021

21. CD8+PD-L1+CXCR3+ polyfunctional T cell abundances are associated with survival in critical SARS-CoV-2–infected patients

Author: Adam, Lucille, primary, Rosenbaum, Pierre, additional, Quentric, Paul, additional, Parizot, Christophe, additional, Bonduelle, Olivia, additional, Guillou, Noëlline, additional, Corneau, Aurélien, additional, Dorgham, Karim, additional, Miyara, Makoto, additional, Luyt, Charles-Edouard, additional, Guihot, Amélie, additional, Gorochov, Guy, additional, Combadière, Christophe, additional, and Combadière, Behazine, additional
Published: 2021
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22. Will Mesenchymal Stromal Cells become tools for immunomodulation in Myasthenia Gravis ?

Author: Bayer Wildberger, Alexandra, Villegas, José, Verdier, Julien, Giannini, Mariette, Maillard, Solène, Truffault, Frédérique, Corneau, Aurélien, Dragin, Nadine, Vanneaux, Valérie, Noël, Danièle, Martinaud, Christophe, Le Panse, Rozen, Berrih- Aknin, Sonia, Vilquin, Jean-Thomas, Centre de Recherche en Myologie, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Plateforme Cytométrie Pitié-Salpêtrière (LUMIC-CYPS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Unité Mixte de Service d'Imagerie et de Cytométrie [CHU Saint-Antoine] (UMS LUMIC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), INSERM, U1160, Département de Gastroentérologie, Université Paris Diderot - Paris 7 (UPD7), Université Paris Diderot, Sorbonne Paris Cité, Paris, France, Bio2M team (Inserm U1183), Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), INSERM U1197, Centre de recherche en Myologie – U974 SU-INSERM, Unité Mixte de Service d'Imagerie et de Cytométrie [CHU Saint-Antoine] (UMS LUMIC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), and Vilquin, Jean-Thomas
Subjects: [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2021

23. Progressive and Coordinated Mobilization of the Skeletal Muscle Niche throughout Tissue Repair Revealed by Single-Cell Proteomic Analysis

Author: Borok, Matthew, Didier, Nathalie, Gattazzo, Francesca, Ozturk, Teoman, Corneau, Aurélien, Rouard, Helene, Relaix, Frédéric, Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Cytométrie Pitié-Salpêtrière (PASS-CYPS), Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé (PASS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), École nationale vétérinaire d'Alfort (ENVA), Etablissement Français du Sang (EFS), Créteil, France., APHP, Hopitaux Universitaires Henri Mondor, DHU Pepsy & Centre de Référence des Maladies Neuromusculaires GNMH, Créteil, France, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), and École nationale vétérinaire - Alfort (ENVA)
Subjects: Proteomics, satellite cells, muscle niche, Wound Healing, Proteome, Stem Cells, [SDV]Life Sciences [q-bio], Muscle Development, Article, Mice, muscle stem cells, lcsh:Biology (General), regeneration, Animals, Cell Lineage, CyTOF, Single-Cell Analysis, skeletal muscle, Muscle, Skeletal, lcsh:QH301-705.5, ComputingMilieux_MISCELLANEOUS
Abstract: Background: Skeletal muscle is one of the only mammalian tissues capable of rapid and efficient regeneration after trauma or in pathological conditions. Skeletal muscle regeneration is driven by the muscle satellite cells, the stem cell population in interaction with their niche. Upon injury, muscle fibers undergo necrosis and muscle stem cells activate, proliferate and fuse to form new myofibers. In addition to myogenic cell populations, interaction with other cell types such as inflammatory cells, mesenchymal (fibroadipogenic progenitors—FAPs, pericytes) and vascular (endothelial) lineages are important for efficient muscle repair. While the role of the distinct populations involved in skeletal muscle regeneration is well characterized, the quantitative changes in the muscle stem cell and niche during the regeneration process remain poorly characterized. Methods: We have used mass cytometry to follow the main muscle cell types (muscle stem cells, vascular, mesenchymal and immune cell lineages) during early activation and over the course of muscle regeneration at D0, D2, D5 and D7 compared with uninjured muscles. Results: Early activation induces a number of rapid changes in the proteome of multiple cell types. Following the induction of damage, we observe a drastic loss of myogenic, vascular and mesenchymal cell lineages while immune cells invade the damaged tissue to clear debris and promote muscle repair. Immune cells constitute up to 80% of the mononuclear cells 5 days post-injury. We show that muscle stem cells are quickly activated in order to form new myofibers and reconstitute the quiescent muscle stem cell pool. In addition, our study provides a quantitative analysis of the various myogenic populations during muscle repair. Conclusions: We have developed a mass cytometry panel to investigate the dynamic nature of muscle regeneration at a single-cell level. Using our panel, we have identified early changes in the proteome of stressed satellite and niche cells. We have also quantified changes in the major cell types of skeletal muscle during regeneration and analyzed myogenic transcription factor expression in satellite cells throughout this process. Our results highlight the progressive dynamic shifts in cell populations and the distinct states of muscle stem cells adopted during skeletal muscle regeneration. Our findings give a deeper understanding of the cellular and molecular aspects of muscle regeneration.
Published: 2021

24. Immune checkpoint inhibitors increase T cell immunity during SARS-CoV-2 infection

Author: Yatim, Nader, primary, Boussier, Jeremy, additional, Tetu, Pauline, additional, Smith, Nikaïa, additional, Bruel, Timothée, additional, Charbit, Bruno, additional, Barnabei, Laura, additional, Corneau, Aurélien, additional, Da Meda, Laetitia, additional, Allayous, Clara, additional, Baroudjian, Barouyr, additional, Jebali, Majdi, additional, Herms, Florian, additional, Grzelak, Ludivine, additional, Staropoli, Isabelle, additional, Calmettes, Vincent, additional, Hadjadj, Jerome, additional, Peyrony, Olivier, additional, Cassius, Charles, additional, LeGoff, Jerome, additional, Kramkimel, Nora, additional, Aractingi, Selim, additional, Fontes, Magnus, additional, Blanc, Catherine, additional, Rieux-Laucat, Frederic, additional, Schwartz, Olivier, additional, Terrier, Benjamin, additional, Duffy, Darragh, additional, and Lebbé, Celeste, additional
Published: 2021
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25. Mass Cytometry: a robust platform for the comprehensive immunomonitoring of CAR‐T‐cell therapies

Author: Corneau, Aurélien, primary, Parizot, Christophe, additional, Cherai, Mustapha, additional, Todesco, Eve, additional, Blanc, Catherine, additional, Litvinova, Elena, additional, Nguyen, Stéphanie, additional, Roos‐Weil, Damien, additional, Guihot, Amélie, additional, and Norol, Francoise, additional
Published: 2021
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26. Inborn errors of type I IFN immunity in patients with life-threatening COVID-19

Author: Zhang, Q, Bastard, P, Liu, Z, Le Pen, J, Moncada-Velez, M, Chen, J, Ogishi, M, Sabli, I, Hodeib, S, Korol, C, Rosain, J, Bilguvar, K, Ye, J, Bolze, A, Bigio, B, Yang, R, Arias, A, Zhou, Q, Zhang, Y, Onodi, F, Korniotis, S, Karpf, L, Philippot, Q, Chbihi, M, Bonnet-Madin, L, Dorgham, K, Smith, N, Schneider, W, Razooky, B, Hoffmann, H, Michailidis, E, Moens, L, Han, J, Lorenzo, L, Bizien, L, Meade, P, Neehus, A, Ugurbil, A, Corneau, A, Kerner, G, Zhang, P, Rapaport, F, Seeleuthner, Y, Manry, J, Masson, C, Schmitt, Y, Schlüter, A, Le Voyer, T, Khan, T, Li, J, Fellay, J, Roussel, L, Shahrooei, M, Alosaimi, M, Mansouri, D, Al-Saud, H, Al-Mulla, F, Almourfi, F, Al-Muhsen, S, Alsohime, F, Al Turki, S, Hasanato, R, van de Beek, D, Biondi, A, Bettini, L, D'Angio, M, Bonfanti, P, Imberti, L, Sottini, A, Paghera, S, Quiros-Roldan, E, Rossi, C, Oler, A, Tompkins, M, Alba, C, Vandernoot, I, Goffard, J, Smits, G, Migeotte, I, Haerynck, F, Soler-Palacin, P, Martin-Nalda, A, Colobran, R, Morange, P, Keles, S, Çölkesen, F, Ozcelik, T, Yasar, K, Senoglu, S, Karabela, Ş, Gallego, C, Novelli, G, Hraiech, S, Tandjaoui-Lambiotte, Y, Duval, X, Laouénan, C, Snow, A, Dalgard, C, Milner, J, Vinh, D, Mogensen, T, Marr, N, Spaan, A, Boisson, B, Boisson-Dupuis, S, Bustamante, J, Puel, A, Ciancanelli, M, Meyts, I, Maniatis, T, Soumelis, V, Amara, A, Nussenzweig, M, García-Sastre, A, Krammer, F, Pujol, A, Duffy, D, Lifton, R, Zhang, S, Gorochov, G, Béziat, V, Jouanguy, E, Sancho-Shimizu, V, Rice, C, Abel, L, Notarangelo, L, Cobat, A, Su, H, Casanova, J, Pesci, A, Zhang, Qian, Bastard, Paul, Liu, Zhiyong, Le Pen, Jérémie, Moncada-Velez, Marcela, Chen, Jie, Ogishi, Masato, Sabli, Ira K D, Hodeib, Stephanie, Korol, Cecilia, Rosain, Jérémie, Bilguvar, Kaya, Ye, Junqiang, Bolze, Alexandre, Bigio, Benedetta, Yang, Rui, Arias, Andrés Augusto, Zhou, Qinhua, Zhang, Yu, Onodi, Fanny, Korniotis, Sarantis, Karpf, Léa, Philippot, Quentin, Chbihi, Marwa, Bonnet-Madin, Lucie, Dorgham, Karim, Smith, Nikaïa, Schneider, William M, Razooky, Brandon S, Hoffmann, Hans-Heinrich, Michailidis, Eleftherios, Moens, Leen, Han, Ji Eun, Lorenzo, Lazaro, Bizien, Lucy, Meade, Philip, Neehus, Anna-Lena, Ugurbil, Aileen Camille, Corneau, Aurélien, Kerner, Gaspard, Zhang, Peng, Rapaport, Franck, Seeleuthner, Yoann, Manry, Jeremy, Masson, Cecile, Schmitt, Yohann, Schlüter, Agatha, Le Voyer, Tom, Khan, Taushif, Li, Juan, Fellay, Jacques, Roussel, Lucie, Shahrooei, Mohammad, Alosaimi, Mohammed F, Mansouri, Davood, Al-Saud, Haya, Al-Mulla, Fahd, Almourfi, Feras, Al-Muhsen, Saleh Zaid, Alsohime, Fahad, Al Turki, Saeed, Hasanato, Rana, van de Beek, Diederik, Biondi, Andrea, Bettini, Laura Rachele, D'Angio, Mariella, Bonfanti, Paolo, Imberti, Luisa, Sottini, Alessandra, Paghera, Simone, Quiros-Roldan, Eugenia, Rossi, Camillo, Oler, Andrew J, Tompkins, Miranda F, Alba, Camille, Vandernoot, Isabelle, Goffard, Jean-Christophe, Smits, Guillaume, Migeotte, Isabelle, Haerynck, Filomeen, Soler-Palacin, Pere, Martin-Nalda, Andrea, Colobran, Roger, Morange, Pierre-Emmanuel, Keles, Sevgi, Çölkesen, Fatma, Ozcelik, Tayfun, Yasar, Kadriye Kart, Senoglu, Sevtap, Karabela, Şemsi Nur, Gallego, Carlos Rodríguez, Novelli, Giuseppe, Hraiech, Sami, Tandjaoui-Lambiotte, Yacine, Duval, Xavier, Laouénan, Cédric, Snow, Andrew L, Dalgard, Clifton L, Milner, Joshua, Vinh, Donald C, Mogensen, Trine H, Marr, Nico, Spaan, András N, Boisson, Bertrand, Boisson-Dupuis, Stéphanie, Bustamante, Jacinta, Puel, Anne, Ciancanelli, Michael, Meyts, Isabelle, Maniatis, Tom, Soumelis, Vassili, Amara, Ali, Nussenzweig, Michel, García-Sastre, Adolfo, Krammer, Florian, Pujol, Aurora, Duffy, Darragh, Lifton, Richard, Zhang, Shen-Ying, Gorochov, Guy, Béziat, Vivien, Jouanguy, Emmanuelle, Sancho-Shimizu, Vanessa, Rice, Charles M, Abel, Laurent, Notarangelo, Luigi D, Cobat, Aurélie, Su, Helen C, Casanova, Jean-Laurent, Pesci, Alberto, Zhang, Q, Bastard, P, Liu, Z, Le Pen, J, Moncada-Velez, M, Chen, J, Ogishi, M, Sabli, I, Hodeib, S, Korol, C, Rosain, J, Bilguvar, K, Ye, J, Bolze, A, Bigio, B, Yang, R, Arias, A, Zhou, Q, Zhang, Y, Onodi, F, Korniotis, S, Karpf, L, Philippot, Q, Chbihi, M, Bonnet-Madin, L, Dorgham, K, Smith, N, Schneider, W, Razooky, B, Hoffmann, H, Michailidis, E, Moens, L, Han, J, Lorenzo, L, Bizien, L, Meade, P, Neehus, A, Ugurbil, A, Corneau, A, Kerner, G, Zhang, P, Rapaport, F, Seeleuthner, Y, Manry, J, Masson, C, Schmitt, Y, Schlüter, A, Le Voyer, T, Khan, T, Li, J, Fellay, J, Roussel, L, Shahrooei, M, Alosaimi, M, Mansouri, D, Al-Saud, H, Al-Mulla, F, Almourfi, F, Al-Muhsen, S, Alsohime, F, Al Turki, S, Hasanato, R, van de Beek, D, Biondi, A, Bettini, L, D'Angio, M, Bonfanti, P, Imberti, L, Sottini, A, Paghera, S, Quiros-Roldan, E, Rossi, C, Oler, A, Tompkins, M, Alba, C, Vandernoot, I, Goffard, J, Smits, G, Migeotte, I, Haerynck, F, Soler-Palacin, P, Martin-Nalda, A, Colobran, R, Morange, P, Keles, S, Çölkesen, F, Ozcelik, T, Yasar, K, Senoglu, S, Karabela, Ş, Gallego, C, Novelli, G, Hraiech, S, Tandjaoui-Lambiotte, Y, Duval, X, Laouénan, C, Snow, A, Dalgard, C, Milner, J, Vinh, D, Mogensen, T, Marr, N, Spaan, A, Boisson, B, Boisson-Dupuis, S, Bustamante, J, Puel, A, Ciancanelli, M, Meyts, I, Maniatis, T, Soumelis, V, Amara, A, Nussenzweig, M, García-Sastre, A, Krammer, F, Pujol, A, Duffy, D, Lifton, R, Zhang, S, Gorochov, G, Béziat, V, Jouanguy, E, Sancho-Shimizu, V, Rice, C, Abel, L, Notarangelo, L, Cobat, A, Su, H, Casanova, J, Pesci, A, Zhang, Qian, Bastard, Paul, Liu, Zhiyong, Le Pen, Jérémie, Moncada-Velez, Marcela, Chen, Jie, Ogishi, Masato, Sabli, Ira K D, Hodeib, Stephanie, Korol, Cecilia, Rosain, Jérémie, Bilguvar, Kaya, Ye, Junqiang, Bolze, Alexandre, Bigio, Benedetta, Yang, Rui, Arias, Andrés Augusto, Zhou, Qinhua, Zhang, Yu, Onodi, Fanny, Korniotis, Sarantis, Karpf, Léa, Philippot, Quentin, Chbihi, Marwa, Bonnet-Madin, Lucie, Dorgham, Karim, Smith, Nikaïa, Schneider, William M, Razooky, Brandon S, Hoffmann, Hans-Heinrich, Michailidis, Eleftherios, Moens, Leen, Han, Ji Eun, Lorenzo, Lazaro, Bizien, Lucy, Meade, Philip, Neehus, Anna-Lena, Ugurbil, Aileen Camille, Corneau, Aurélien, Kerner, Gaspard, Zhang, Peng, Rapaport, Franck, Seeleuthner, Yoann, Manry, Jeremy, Masson, Cecile, Schmitt, Yohann, Schlüter, Agatha, Le Voyer, Tom, Khan, Taushif, Li, Juan, Fellay, Jacques, Roussel, Lucie, Shahrooei, Mohammad, Alosaimi, Mohammed F, Mansouri, Davood, Al-Saud, Haya, Al-Mulla, Fahd, Almourfi, Feras, Al-Muhsen, Saleh Zaid, Alsohime, Fahad, Al Turki, Saeed, Hasanato, Rana, van de Beek, Diederik, Biondi, Andrea, Bettini, Laura Rachele, D'Angio, Mariella, Bonfanti, Paolo, Imberti, Luisa, Sottini, Alessandra, Paghera, Simone, Quiros-Roldan, Eugenia, Rossi, Camillo, Oler, Andrew J, Tompkins, Miranda F, Alba, Camille, Vandernoot, Isabelle, Goffard, Jean-Christophe, Smits, Guillaume, Migeotte, Isabelle, Haerynck, Filomeen, Soler-Palacin, Pere, Martin-Nalda, Andrea, Colobran, Roger, Morange, Pierre-Emmanuel, Keles, Sevgi, Çölkesen, Fatma, Ozcelik, Tayfun, Yasar, Kadriye Kart, Senoglu, Sevtap, Karabela, Şemsi Nur, Gallego, Carlos Rodríguez, Novelli, Giuseppe, Hraiech, Sami, Tandjaoui-Lambiotte, Yacine, Duval, Xavier, Laouénan, Cédric, Snow, Andrew L, Dalgard, Clifton L, Milner, Joshua, Vinh, Donald C, Mogensen, Trine H, Marr, Nico, Spaan, András N, Boisson, Bertrand, Boisson-Dupuis, Stéphanie, Bustamante, Jacinta, Puel, Anne, Ciancanelli, Michael, Meyts, Isabelle, Maniatis, Tom, Soumelis, Vassili, Amara, Ali, Nussenzweig, Michel, García-Sastre, Adolfo, Krammer, Florian, Pujol, Aurora, Duffy, Darragh, Lifton, Richard, Zhang, Shen-Ying, Gorochov, Guy, Béziat, Vivien, Jouanguy, Emmanuelle, Sancho-Shimizu, Vanessa, Rice, Charles M, Abel, Laurent, Notarangelo, Luigi D, Cobat, Aurélie, Su, Helen C, Casanova, Jean-Laurent, and Pesci, Alberto
Abstract: Clinical outcome upon infection with SARS-CoV-2 ranges from silent infection to lethal COVID-19. We have found an enrichment in rare variants predicted to be loss-of-function (LOF) at the 13 human loci known to govern TLR3- and IRF7-dependent type I interferon (IFN) immunity to influenza virus, in 659 patients with life-threatening COVID-19 pneumonia, relative to 534 subjects with asymptomatic or benign infection. By testing these and other rare variants at these 13 loci, we experimentally define LOF variants in 23 patients (3.5%), aged 17 to 77 years, underlying autosomal recessive or dominant deficiencies. We show that human fibroblasts with mutations affecting this pathway are vulnerable to SARS-CoV-2. Inborn errors of TLR3- and IRF7-dependent type I IFN immunity can underlie life-threatening COVID-19 pneumonia in patients with no prior severe infection.
Published: 2020

27. Inborn errors of type I IFN immunity in patients with life-threatening COVID-19

Author: Zhang, Qian [0000-0002-9040-3289], Bastard, Paul [0000-0002-5926-8437], Le Pen, Jeremie [0000-0001-7025-9526], Moncada-Velez, Marcela [0000-0002-3073-5345], Ogishi, Masato [0000-0003-2421-7389], Sabli, Ira K. D. [0000-0002-0170-2990], Hodeib, Stephanie [0000-0002-5978-6189], Korol, Cecilia [0000-0002-0023-8823], Bilguvar, Kaya [0000-0002-7313-7652], Bolze, Alexandre [0000-0001-7399-2766], Bigio, Benedetta [0000-0001-7291-5638], Yang, Rui [0000-0003-4427-2158], Arias, Andrés Augusto [0000-0002-9478-8403], Zhou, Qinhua [0000-0002-5112-3727], Chbihi, Marwa [0000-0002-2771-851X], Bonnet-Madin, Lucie [0000-0002-9848-3287], Dorgham, Karim [0000-0001-9539-3203], Smith, Nikaïa [0000-0002-0202-612X], Schneider, William M. [0000-0001-9407-6118], Razooky, Brandon S. [0000-0002-5263-1512], Hoffmann, Hans-Heinrich [0000-0003-0554-0244], Michailidis, Eleftherios [0000-0002-9907-4346], Han, Jin Eun [0000-0003-1112-9320], Lorenzo, Lazaro [0000-0001-6648-8684], Bizien, Lucy [0000-0001-9163-9122], Meade, Philip [0000-0002-6754-7209], Neehus, Anna-Lena [0000-0002-8573-6820], Ugurbil, Aileen Camille [0000-0002-9450-3092], Kerner, Gaspard [0000-0003-0146-9428], Zhang, Peng [0000-0002-6129-567X], Rapaport, Franck [0000-0001-6553-2110], Manry, Jérémy [0000-0001-5998-2051], Masson, Cecile [0000-0001-7870-7821], Schlüter, Agatha [0000-0001-6732-1528], Le Voyer, Tom [0000-0002-0630-8626], Khan, Taushif [0000-0002-7917-8965], Fellay, Jacques [0000-0002-8240-939X], Roussel, Lucie [0000-0001-5355-702X], Alosaimi, Mohammed F. [0000-0002-8025-3491], Al-Mulla, Fahd [0000-0001-5409-3829], Almourfi, Feras [0000-0002-5166-4662], Alsohime, Fahad [0000-0002-4979-3895], Al Turki, Saeed [0000-0001-7017-336X], Hasanato, Rana [0000-0002-4697-2222], Beek, Diederik van der [0000-0002-4571-044X], Bettini, Laura Rachele [0000-0002-0280-1704], Bonfanti, Paolo [0000-0001-7289-8823], Oler, Andrew J. [0000-0002-6310-0434], Tompkins, Miranda F. [0000-0003-2941-7515], Alba, Camille [0000-0002-0458-1629], Smits, Guillaume [0000-0003-2845-6758], Soler-Palacín, Pere [0000-0002-0346-5570], Martin-Nalda, Andrea [0000-0002-3590-0186], Colobran, Roger [0000-0002-5964-536X], Çölkesen, Fatma [0000-0001-9545-5179], Yasar, Kadriye Kart [0000-0003-2963-4894], Senoglu, Sevtap [0000-0003-4796-9583], Karabela, Şemsi Nur [0000-0003-2562-3004], Rodríguez-Gallego, Carlos [0000-0002-4344-8644], Novelli, Giuseppe [0000-0002-7781-602X], Tandjaoui-Lambiotte, Yacine [0000-0003-1123-4788], Laouénan, Cédric [0000-0002-3681-6314], Zhang, Qian, Bastard, Paul, Liu, Zhiyong, Le Pen, Jeremie, Moncada-Velez, Marcela, Chen, Jie, Ogishi, Masato, Sabli, Ira K. D., Hodeib, Stephanie, Korol, Cecilia, Rosain, Jérémie, Rodríguez-Gallego, Carlos, Novelli, Giuseppe, Hraiech, Sami, COVID Human Genetic Effort, Tandjaoui-Lambiotte, Yacine, Duval, Xavier, Ciancanelli, Michael J., Laouénan, Cédric, COVID-STORM Clinicians, COVID Clinicians, Gorochov, Guy, Imagine COVID Group, French COVID Cohort Study Group, NIAID-USUHS/TAGC COVID Immunity Group, Snow, Andrew L., Dalgard, Clifton L., Milner, Joshua D., Vinh, Donald C., Meyts, Isabelle, Mogensen, Trine, Marr, Nico, Béziat, Vivien, Bilguvar, Kaya, Spaan, András N., Boisson, Bertrand, Boisson-Dupuis, Stéphanie, Bustamante, Jacinta, Maniatis, Tom, Soumelis, Vassili, Amara, Ali, Ye, Junqiang, Nussenzweig, Michel C., Jouanguy, Emmanuelle, García-Sastre, Adolfo, Krammer, Florian, Smith, Nikaïa, Pujol, Aurora, Duffy, Darragh, Lifton, Richard P., Zhang, Shen-Ying, Bolze, Alexandre, Sancho-Shimizu, Vanessa, Rice, Charles M., Schneider, William M., Abel, Laurent, Notarangelo, Luigi D., Cobat, Aurélie, Zhang, Peng, Su, Helen C., Casanova, Jean-Laurent, Bigio, Benedetta, Yang, Rui, Arias, Andrés Augusto, Zhou, Qinhua, Zhang, Yu, Razooky, Brandon S., Onodi, Fanny, Korniotis, Sarantis, Rapaport, Franck, Karpf, Lea, Philippot, Quentin, Chbihi, Marwa, Bonnet-Madin, Lucie, Dorgham, Karim, Hoffmann, Hans-Heinrich, Michailidis, Eleftherios, Moens, Leen, Han, Jin Eun, Lorenzo, Lazaro, Seeleuthner, Yoann, Bizien, Lucy, Meade, Philip, Biondi, Andrea, Neehus, Anna-Lena, Ugurbil, Aileen Camille, Corneau, Aurélien, Kerner, Gaspard, Manry, Jérémy, Masson, Cecile, Schmitt, Yoann, Bettini, Laura Rachele, Schlüter, Agatha, Le Voyer, Tom, Khan, Taushif, Smits, Guillaume, Li, Juan, Fellay, Jacques, Roussel, Lucie, Shahrooei, Mohammad, Alosaimi, Mohammed F., Mansouri, Davood, Al-Saud, Haya, D'Angio, Mariella, Al-Mulla, Fahd, Almourfi, Feras, Migeotte, Isabelle, Al-Muhsen, Saleh Zaid, Alsohime, Fahad, Al Turki, Saeed, Hasanato, Rana, Beek, Diederik van der, Bonfanti, Paolo, Imberti, Luisa, Sottini, Alessandra, Paghera, Simone, Quiros-Roldan, Eugenia, Haerynck, Filomeen, Rossi, Camillo, Oler, Andrew J., CoV-Contact Cohort, Tompkins, Miranda F., Alba, Camille, Vandernoot, Isabelle, Goffard, Jean-Christophe, Soler-Palacín, Pere, Martin-Nalda, Andrea, Colobran, Roger, Amsterdam UMC Covid-19 Biobank, Morange, Pierre-Emmanuel, Keles, Sevgi, Çölkesen, Fatma, Puel, Anne, Ozcelik, Tayfun, Yasar, Kadriye Kart, Senoglu, Sevtap, Karabela, Şemsi Nur, Zhang, Qian [0000-0002-9040-3289], Bastard, Paul [0000-0002-5926-8437], Le Pen, Jeremie [0000-0001-7025-9526], Moncada-Velez, Marcela [0000-0002-3073-5345], Ogishi, Masato [0000-0003-2421-7389], Sabli, Ira K. D. [0000-0002-0170-2990], Hodeib, Stephanie [0000-0002-5978-6189], Korol, Cecilia [0000-0002-0023-8823], Bilguvar, Kaya [0000-0002-7313-7652], Bolze, Alexandre [0000-0001-7399-2766], Bigio, Benedetta [0000-0001-7291-5638], Yang, Rui [0000-0003-4427-2158], Arias, Andrés Augusto [0000-0002-9478-8403], Zhou, Qinhua [0000-0002-5112-3727], Chbihi, Marwa [0000-0002-2771-851X], Bonnet-Madin, Lucie [0000-0002-9848-3287], Dorgham, Karim [0000-0001-9539-3203], Smith, Nikaïa [0000-0002-0202-612X], Schneider, William M. [0000-0001-9407-6118], Razooky, Brandon S. [0000-0002-5263-1512], Hoffmann, Hans-Heinrich [0000-0003-0554-0244], Michailidis, Eleftherios [0000-0002-9907-4346], Han, Jin Eun [0000-0003-1112-9320], Lorenzo, Lazaro [0000-0001-6648-8684], Bizien, Lucy [0000-0001-9163-9122], Meade, Philip [0000-0002-6754-7209], Neehus, Anna-Lena [0000-0002-8573-6820], Ugurbil, Aileen Camille [0000-0002-9450-3092], Kerner, Gaspard [0000-0003-0146-9428], Zhang, Peng [0000-0002-6129-567X], Rapaport, Franck [0000-0001-6553-2110], Manry, Jérémy [0000-0001-5998-2051], Masson, Cecile [0000-0001-7870-7821], Schlüter, Agatha [0000-0001-6732-1528], Le Voyer, Tom [0000-0002-0630-8626], Khan, Taushif [0000-0002-7917-8965], Fellay, Jacques [0000-0002-8240-939X], Roussel, Lucie [0000-0001-5355-702X], Alosaimi, Mohammed F. [0000-0002-8025-3491], Al-Mulla, Fahd [0000-0001-5409-3829], Almourfi, Feras [0000-0002-5166-4662], Alsohime, Fahad [0000-0002-4979-3895], Al Turki, Saeed [0000-0001-7017-336X], Hasanato, Rana [0000-0002-4697-2222], Beek, Diederik van der [0000-0002-4571-044X], Bettini, Laura Rachele [0000-0002-0280-1704], Bonfanti, Paolo [0000-0001-7289-8823], Oler, Andrew J. [0000-0002-6310-0434], Tompkins, Miranda F. [0000-0003-2941-7515], Alba, Camille [0000-0002-0458-1629], Smits, Guillaume [0000-0003-2845-6758], Soler-Palacín, Pere [0000-0002-0346-5570], Martin-Nalda, Andrea [0000-0002-3590-0186], Colobran, Roger [0000-0002-5964-536X], Çölkesen, Fatma [0000-0001-9545-5179], Yasar, Kadriye Kart [0000-0003-2963-4894], Senoglu, Sevtap [0000-0003-4796-9583], Karabela, Şemsi Nur [0000-0003-2562-3004], Rodríguez-Gallego, Carlos [0000-0002-4344-8644], Novelli, Giuseppe [0000-0002-7781-602X], Tandjaoui-Lambiotte, Yacine [0000-0003-1123-4788], Laouénan, Cédric [0000-0002-3681-6314], Zhang, Qian, Bastard, Paul, Liu, Zhiyong, Le Pen, Jeremie, Moncada-Velez, Marcela, Chen, Jie, Ogishi, Masato, Sabli, Ira K. D., Hodeib, Stephanie, Korol, Cecilia, Rosain, Jérémie, Rodríguez-Gallego, Carlos, Novelli, Giuseppe, Hraiech, Sami, COVID Human Genetic Effort, Tandjaoui-Lambiotte, Yacine, Duval, Xavier, Ciancanelli, Michael J., Laouénan, Cédric, COVID-STORM Clinicians, COVID Clinicians, Gorochov, Guy, Imagine COVID Group, French COVID Cohort Study Group, NIAID-USUHS/TAGC COVID Immunity Group, Snow, Andrew L., Dalgard, Clifton L., Milner, Joshua D., Vinh, Donald C., Meyts, Isabelle, Mogensen, Trine, Marr, Nico, Béziat, Vivien, Bilguvar, Kaya, Spaan, András N., Boisson, Bertrand, Boisson-Dupuis, Stéphanie, Bustamante, Jacinta, Maniatis, Tom, Soumelis, Vassili, Amara, Ali, Ye, Junqiang, Nussenzweig, Michel C., Jouanguy, Emmanuelle, García-Sastre, Adolfo, Krammer, Florian, Smith, Nikaïa, Pujol, Aurora, Duffy, Darragh, Lifton, Richard P., Zhang, Shen-Ying, Bolze, Alexandre, Sancho-Shimizu, Vanessa, Rice, Charles M., Schneider, William M., Abel, Laurent, Notarangelo, Luigi D., Cobat, Aurélie, Zhang, Peng, Su, Helen C., Casanova, Jean-Laurent, Bigio, Benedetta, Yang, Rui, Arias, Andrés Augusto, Zhou, Qinhua, Zhang, Yu, Razooky, Brandon S., Onodi, Fanny, Korniotis, Sarantis, Rapaport, Franck, Karpf, Lea, Philippot, Quentin, Chbihi, Marwa, Bonnet-Madin, Lucie, Dorgham, Karim, Hoffmann, Hans-Heinrich, Michailidis, Eleftherios, Moens, Leen, Han, Jin Eun, Lorenzo, Lazaro, Seeleuthner, Yoann, Bizien, Lucy, Meade, Philip, Biondi, Andrea, Neehus, Anna-Lena, Ugurbil, Aileen Camille, Corneau, Aurélien, Kerner, Gaspard, Manry, Jérémy, Masson, Cecile, Schmitt, Yoann, Bettini, Laura Rachele, Schlüter, Agatha, Le Voyer, Tom, Khan, Taushif, Smits, Guillaume, Li, Juan, Fellay, Jacques, Roussel, Lucie, Shahrooei, Mohammad, Alosaimi, Mohammed F., Mansouri, Davood, Al-Saud, Haya, D'Angio, Mariella, Al-Mulla, Fahd, Almourfi, Feras, Migeotte, Isabelle, Al-Muhsen, Saleh Zaid, Alsohime, Fahad, Al Turki, Saeed, Hasanato, Rana, Beek, Diederik van der, Bonfanti, Paolo, Imberti, Luisa, Sottini, Alessandra, Paghera, Simone, Quiros-Roldan, Eugenia, Haerynck, Filomeen, Rossi, Camillo, Oler, Andrew J., CoV-Contact Cohort, Tompkins, Miranda F., Alba, Camille, Vandernoot, Isabelle, Goffard, Jean-Christophe, Soler-Palacín, Pere, Martin-Nalda, Andrea, Colobran, Roger, Amsterdam UMC Covid-19 Biobank, Morange, Pierre-Emmanuel, Keles, Sevgi, Çölkesen, Fatma, Puel, Anne, Ozcelik, Tayfun, Yasar, Kadriye Kart, Senoglu, Sevtap, and Karabela, Şemsi Nur
Abstract: Clinical outcome upon infection with SARS-CoV-2 ranges from silent infection to lethal COVID-19. We have found an enrichment in rare variants predicted to be loss-of-function (LOF) at the 13 human loci known to govern TLR3- and IRF7-dependent type I interferon (IFN) immunity to influenza virus, in 659 patients with life-threatening COVID-19 pneumonia, relative to 534 subjects with asymptomatic or benign infection. By testing these and other rare variants at these 13 loci, we experimentally define LOF variants in 23 patients (3.5%), aged 17 to 77 years, underlying autosomal recessive or dominant deficiencies. We show that human fibroblasts with mutations affecting this pathway are vulnerable to SARS-CoV-2. Inborn errors of TLR3- and IRF7-dependent type I IFN immunity can underlie life-threatening COVID-19 pneumonia in patients with no prior severe infection.
Published: 2020

28. Towards a vaccine against AIDS

Author: Fausther-Bovendo Hugues, Mangeot-Méderle Isabelle, Corneau Aurélien, Dereuddre-Bosquet Nathalie, Vieillard Vincent, Le Grand Roger, and Debre Patrice
Subjects: Immunologic diseases. Allergy, RC581-607
Published: 2010
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29. PLASMACYTOID DENDRITIC CELL DYNAMICS TUNE INTERFERON-ALPHA RESPONSE DURING ACUTE SIV MACAQUE INFECTION: ABSTRACT #26

Author: Bruel, Timothée, Démoulins, Thomas, Kreutz, Christine, Dutrieux, Jacques, Cosma, Antonio, Corneau, Aurélien, Delache, Benoit, Torres, Claire, Dupuy, Stéphanie, Cheynier, Rémi, Bosquet, Nathalie, Le Grand, Roger, and Vaslin, Bruno
Published: 2013

30. LOSS AND REGAIN OF SIV CONTROL UPON CD8+ CELL DEPLETION IN VIVO IN SIV-CONTROLLER MACAQUES IS NOT ASSOCIATED WITH EFFICIENT SIV SPECIFIC CD8+ T-CELL RESPONSES: ABSTRACT #6

Author: Saez-Cirion, Asier, Bruel, Timothée, Hamimi, Chiraz, Dereuddre-Bosquet, Nathalie, Cosma, Antonio, Shin, So Youn, Corneau, Aurélien, Versmisse, Pierre, Torres, Claire, Delache, Benoît, Even, Sophie, Guenounou, Sabrina, Targat, Brice, Malleret, Benoît, Karlsson, Ingrid, Barré-Sinoussi, Françoise, Le Grand, Roger, Pancino, Gianfranco, and Vaslin, Bruno
Published: 2013

31. Impaired type I interferon activity and inflammatory responses in severe COVID-19 patients

Author: Hadjadj, Jérôme, primary, Yatim, Nader, additional, Barnabei, Laura, additional, Corneau, Aurélien, additional, Boussier, Jeremy, additional, Smith, Nikaïa, additional, Péré, Hélène, additional, Charbit, Bruno, additional, Bondet, Vincent, additional, Chenevier-Gobeaux, Camille, additional, Breillat, Paul, additional, Carlier, Nicolas, additional, Gauzit, Rémy, additional, Morbieu, Caroline, additional, Pène, Frédéric, additional, Marin, Nathalie, additional, Roche, Nicolas, additional, Szwebel, Tali-Anne, additional, Merkling, Sarah H., additional, Treluyer, Jean-Marc, additional, Veyer, David, additional, Mouthon, Luc, additional, Blanc, Catherine, additional, Tharaux, Pierre-Louis, additional, Rozenberg, Flore, additional, Fischer, Alain, additional, Duffy, Darragh, additional, Rieux-Laucat, Frédéric, additional, Kernéis, Solen, additional, and Terrier, Benjamin, additional
Published: 2020
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32. Two new immature and dysfunctional neutrophil cell subsets define a predictive signature of sepsis useable in clinical practice

Author: Meghraoui-Kheddar, Aïda, primary, Chousterman, Benjamin G., additional, Guillou, Noëlline, additional, Barone, Sierra M., additional, Granjeaud, Samuel, additional, Vallet, Helene, additional, Corneau, Aurélien, additional, Guessous, Karim, additional, Boissonnas, Alexandre, additional, Irish, Jonathan M., additional, and Combadière, Christophe, additional
Published: 2020
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33. Immune phenotyping of Erdheim-Chester disease through mass cytometry highlights decreased proportion of non-classical monocytes and increased proportion of Th17 cells

Author: Papo, Matthias, primary, Corneau, Aurélien, additional, Cohen-Aubart, Fleur, additional, Robin, Brice, additional, Emile, Jean-François, additional, Miyara, Makoto, additional, Blanc, Catherine, additional, Amoura, Zahir, additional, Hermine, Olivier, additional, Haroche, Julien, additional, and Trovati Maciel, Thiago, additional
Published: 2020
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34. Impaired type I interferon activity and exacerbated inflammatory responses in severe Covid-19 patients

Author: Hadjadj, Jérôme, primary, Yatim, Nader, additional, Barnabei, Laura, additional, Corneau, Aurélien, additional, Boussier, Jeremy, additional, Péré, Hélène, additional, Charbit, Bruno, additional, Bondet, Vincent, additional, Chenevier-Gobeaux, Camille, additional, Breillat, Paul, additional, Carlier, Nicolas, additional, Gauzit, Rémy, additional, Morbieu, Caroline, additional, Pène, Frédéric, additional, Marin, Nathalie, additional, Roche, Nicolas, additional, Szwebel, Tali-Anne, additional, Smith, Nikaïa, additional, Merkling, Sarah H, additional, Treluyer, Jean-Marc, additional, Verer, David, additional, Mouthon, Luc, additional, Blanc, Catherine, additional, Tharaux, Pierre-Louis, additional, Rozenberg, Flore, additional, Fischer, Alain, additional, Duffy, Darragh, additional, Rieux-Laucat, Frédéric, additional, Kernéis, Solen, additional, and Terrier, Benjamin, additional
Published: 2020
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35. New approaches to multiparametric analysis in mass cytometry

Author: Corneau, Aurélien, STAR, ABES, CORNEAU, Aurélien, Cytométrie Pitié-Salpêtrière (PASS-CYPS), Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé (PASS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), PSL Research University, Brigitte Autran, Véronique Frachet, Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Université Paris sciences et lettres, and Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Marqueurs d’épuisement cellulaire, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.IMM] Life Sciences [q-bio]/Immunology, Activation, Hiv, Vih, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Analyse multiparamétrique, Cycle cellulaire, Multiparametric analysis, Cell cycle, Marqueurs d'épuisement cellulaire, Activation cellulaire, Cytométrie de masse, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, Cellular activation, [SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology, Cell cyle, [SDV.IMM.IA] Life Sciences [q-bio]/Immunology/Adaptive immunology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.IMM]Life Sciences [q-bio]/Immunology, Mass cytometry, Immune-Checkpoint, [SDV.BC] Life Sciences [q-bio]/Cellular Biology
Abstract: Mass Cytometry (MCM) has revolutionized the study of cell and phenotypic diversity, significantly increasing the number of markers that can be analyzed simultaneously (41 to date). By making it possible to precisely define the status of lymphocyte populations, particularly with regard to their differentiation, activation and entry into the cell cycle, CMM has uncovered small subsets previously unknown. In this study, MMC was used to try to better characterize HIV reservoirs. With the introduction of combination antiretroviral therapy (ART) in 1996, HIV infection has been transformed from a fatal fate to a manageable chronic disease with a normal lifespan due to a reduction in active viral replication (the amount of virus is below optimal detection limits). However, if treatment is interrupted, the patient's viral load increases again due to viable provirus reservoirs located in long-lived cell populations that cannot be eliminated by current therapies. These infected reservoir cells are a major obstacle to the eradication of HIV. The best characterized reservoir is that of CD4+ T lymphocytes and is mainly hosted in MCT, MST, MSCT, and Tfh. An initial study allowed us to evaluate the stages of the cell cycle in association with markers of differentiation, activation and depletion, leading to an in-depth evaluation of the quiescence status of CD4 T cells likely to harbour latent HIV reservoirs. This broad multiplex analysis demonstrates that certain subsets of LTCD4+CD25-HLA-DR - classically considered "at rest" - actually contain significant amounts of cells cycling or expressing inhibitory receptors, opening new avenues for redefining quiescent CD4 T cells in peripheral blood. A second study aimed to define in vivo HIV-producing CD4 LT populations. We developed a multiparametric analysis on cells from HIV+ patients on ART and in the therapeutic interruption phase (TIA). This study shows that CD3+CD4+CD32high cells express a high level of activation markers and receive important activation signals via cytokines, in contrast to CD32a cells. On the other hand, the analysis of HIV-producing LTCD4+ (expressing the p24 capsid protein), allowed us to detect a very low number of p24+ positive cells (less than 0.004% in ATI phase but none before). The phenotype of the producing cells was then highlighted. They are T lymphocytes that do not express CD8, enriched with a factor 4 in TSCM cells and a factor 2 in TFH. These populations are highly enriched in activated cells co-expressing 3 activation markers (increased by a factor of 20) and are cycling (Ki67+) and/or over-expressing immune control molecules (ICP) with an enrichment of a factor of 500. This allows us to detect producer cells with much higher frequencies in these TCD3+CD8- populations in cycle up to 0.08%, and in G2 phase (2.46%), but also in cells showing poly-expression of the 4 immune-checkpoints (2.27%). The advent of mass cytometry has exponentially increased the information we could obtain on a cell. Thanks to this tool, the identification of the cell cycle, in correlation with different phenotypic markers, makes it possible to explore previously inaccessible information, including the analysis of the latent and productive reservoirs of HIV. This work thus makes it possible to characterize as precisely as possible these HIV-producing cells, but also latent cells, and potential reservoirs of the virus., La cytométrie de masse CMM) a révolutionné l'étude de la diversité cellulaire et phénotypique, en augmentant de manière significative le nombre de marqueurs pouvant être analysés simultanément (41 à ce jour). En permettant de définir précisément l'état des populations de lymphocytes, notamment en ce qui concerne leur différenciation, activation et leur entrée dans le cycle cellulaire, la CMM a mis au jour de petits sous-ensembles jusqu'ici inconnus. Dans cette étude, la CMM a été utilisée pour tenter de mieux caractériser les réservoirs du VIH. Avec l'introduction de la thérapie antirétrovirale combinée (ART) en 1996, l'infection par le VIH est passée d'un destin fatal à une maladie chronique gérable avec une durée de vie normale grâce à une réduction de la réplication virale active (la quantité de virus est en deçà des limites de détection optimales). Cependant, si le traitement est interrompu, la charge virale chez le patient augmente à nouveau du fait des réservoirs de provirus viables localisés dans des populations de cellules à longue durée de vie et qui ne peuvent pas être éliminées par les traitements actuels. Ces cellules infectées réservoirs constituent un obstacle majeur à l'éradication du VIH. Le réservoir le mieux caractérisé est celui des lymphocytes T CD4+ et est principalement hébergé dans les TCM, les TTM, les TSCM et les Tfh. Une première étude nous a permis d’évaluer les stades du cycle cellulaire en association à des marqueurs de différenciation, d'activation et d'épuisement, pour aboutir à une évaluation poussée de l'état de quiescence des lymphocytes T CD4 susceptibles d’abriter les réservoirs latents de VIH. Cette large analyse multiplexe démontre que certains sous-ensembles des LTCD4+CD25-HLA-DR- classiquement considérés "au repos"- contiennent en fait des quantités notables de cellules en cycle ou exprimant des récepteurs inhibiteurs, ouvrant de nouvelles voies pour une redéfinition des cellules T CD4 quiescentes du sang périphérique. Une deuxième étude avait pour but de définir les populations de LT CD4 produisant du VIH in vivo. Nous avons développé une analyse multiparamétrique sur des cellules de patients VIH+ sous ART et en phase d’interruption thérapeutique (ATI). Cette étude met en évidence que les cellules CD3+CD4+CD32high expriment un fort taux de marqueurs d’activation et reçoivent d’importants signaux d’activation via des cytokines, à l'inverse des cellules CD32a-. D'autre part, l'analyse des LTCD4+ producteurs de VIH (exprimant la protéine de capside p24), nous a permis de détecter un très faible nombre de cellules positives p24+ (inférieur à 0,004% en phase d’ATI mais aucun avant). Le phénotype des cellules productrices a ensuite été mis en évidence. Il s’agit de lymphocytes T n’exprimant pas de CD8, enrichis d’un facteur 4 en cellules TSCM, et d'un facteur 2 en TFH. Ces populations sont très enrichies en cellules activées co-exprimant 3 marqueurs d’activation (augmentés d’un facteur 20) et sont en cycle (Ki67+) et/ou sur-expriment des molécules de contrôle immunitaire (ICP) avec un enrichissement d’un facteur 500. Ceci nous permet de détecter des cellules productrices avec des fréquences beaucoup plus élevées dans ces populations TCD3+CD8- en cycle à hauteur de 0,08%, et en phase G2 (2,46%), mais également dans les cellules présentant une poly-expression des 4 immune-checkpoints (2,27%). L’avènement de la cytométrie de masse a augmenté de façon exponentielle les informations que nous pouvions obtenir sur une cellule. Grâce à cet outil, l’identification du cycle cellulaire, en corrélation avec différents marqueurs phénotypiques, permet d’explorer des informations jusque-là inaccessibles, entre autre l’analyse des réservoirs latents et productif du VIH. Ce travail permet ainsi de caractériser le plus précisément possible ces cellules productrices de VIH, mais aussi les cellules latentes, et potentiellement réservoirs du virus.
Published: 2018

36. Phenotypical and functional characterizations of conditioned Mesenchymal Stromal Cells MSC, as tools for immunomodulation in Myasthenia Gravis

Author: Bayer Wildberger, Alexandra, Villegas, José, Verdier, Julien, Giannini, Mariette, Maillard, Solène, Truffault, Frédérique, Corneau, Aurélien, Dragin, Nadine, Vanneaux, Valérie, Rouard, Hélène, Noël, Danièle, Martinaud, Christophe, Le Panse, Rozen, Berrih- Aknin, Sonia, Vilquin, Jean-Thomas, Sorbonne Université (SU), Univ Paris 06, CNRS, Inst Myol, INSERM,UMR 974,FRE 3617, Paris, France, and Partenaires INRAE
Subjects: [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2019

37. Mesenchymal Stromal Cells (MSC) : phenotypical and functional characterizations as tools for immunomodulation in Myasthenia Gravis

Author: Bayer Wildberger, Alexandra, Villegas, José, Verdier, Julien, Giannini, Mariette, Maillard, Solène, Truffault, Frédérique, Corneau, Aurélien, Dragin, Nadine, Vanneaux, Valérie, Rouard, Hélène, Noël, Danièle, Martinaud, Christophe, Le Panse, Rozen, Berrih-Aknin, Sonia, Vilquin, J.-T, Centre de Recherche en Myologie, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Sorbonne Université (SU), Institut de Myologie, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Plateforme Cytométrie Pitié-Salpêtrière (LUMIC-CYPS), Unité Mixte de Service d'Imagerie et de Cytométrie [CHU Saint-Antoine] (UMS LUMIC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Alloimmunité-Autoimmunité-Transplantation (A2T), Institut Universitaire d'Hématologie (IUH), Université Paris Diderot - Paris 7 (UPD7)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de recherche en Myologie – U974 SU-INSERM, and Centre National de la Recherche Scientifique (CNRS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Association française contre les myopathies (AFM-Téléthon)-Sorbonne Université (SU)
Subjects: [SDV.IMM.IMM]Life Sciences [q-bio]/Immunology/Immunotherapy
Abstract: International audience; Several autoimmune diseases are mediated by antibodies produced after deregulations of the immune system and directed against self antigens. Myasthenia Gravis (MG) is a rare disease in which pathogenicity is due to autoantibodies directed against the neuromuscular endplate. MG is not really cured, corticosteroids and azathioprin are commonly used, however they trigger severe side-effects, mandating the setup of novel therapies. Mesenchymal Stromal/Stem Cells (MSC) are multipotent progenitor cells that can be isolated from various human tissues and can modulate the immune system via soluble mediators and cell-cell contacts. Our team has recently validated a new animal model of MG, in which we demonstrated that the transfer of MSC conditioned by peripheral blood mononucleated cells (PBMC) improved the clinical status of the animals (Sudres et al., JCI Insight 2017). To develop this immunomodulating approach in clinical perspective, we compared the phenotypes of research-grade (RG) and clinical-grade (CG) MSC testing a series of 60 antibodies (Ab) directed against surface antigens by flow cytometry. We evaluated the variations introduced by different conditioning treatments (activation by gamma-interferon, cross-stimulation by PBMC or monocytes). Markers involved in immunomodulation (recognition, activation, function of complement, co-stimulation, immune checkpoints) were increased or decreased depending on treatment. Adhesion molecules and receptors were also differentially modified (integrins, selectins, cell-cell adhesion molecules, growth factor receptors, tetraspanins). These results suggest that the conditioning regimens act through different pathways. From this panel, we derived a second one of 31 Ab allowing simultaneous labeling of MSC at single cell level by mass cytometry (CyTOF). We defined MSC clusters which were modulated upon activation. In parallel, we evaluated the functional activity of resting or conditioned CG MSC through a cell proliferation assay, and through the quantification by ELISA of secreted immunomodulating products. The conditioning regimens differentially modulated the secretion of Prostaglandin E2 and TGFbeta1, and inhibited PBMC proliferation. This work unveiled phenotypic and functional markers of MSC along with their modulations according to different treatments, and will contribute to validate a cell therapy product for immunomodulation purposes.
Published: 2019

38. Characterization of circulating immune cells in a rare autoimmune disease: Myasthenia gravis

Author: Verdier, Julien, Truffault, Frédérique, Behin, Anthony, Blanc, Catherine, Le Panse, Rozen, Corneau, Aurélien, Berrih-Aknin, Sonia, Centre de recherche en Myologie – U974 SU-INSERM, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut de Myologie, Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Plateforme cytométrie en flux, Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR113, Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé (PASS), Ecole Doctorale 472 mention « Systèmes intégrés, environnement et biodiversité » (SIEB) (ED 472 SIEB), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Plateforme Cytométrie Pitié-Salpêtrière (LUMIC-CYPS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Unité Mixte de Service d'Imagerie et de Cytométrie [CHU Saint-Antoine] (UMS LUMIC), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)
Subjects: Myasthenia Gravis MG, [SDV]Life Sciences [q-bio], Autoimmunity
Abstract: International audience
Published: 2019

39. High-Dimensional Single-Cell Cartography Reveals Novel Skeletal Muscle-Resident Cell Populations

Author: Giordani, Lorenzo, He, Jiawei, Negroni, Elisa, Sakai, Hiroshi, Law, Yiu Chun, Siu, Men Yi Mona, Wan, Raymond, Corneau, Aurélien, Tajbakhsh, Shahragim, Cheung, Hiu Tung, Le grand, Fabien, Giordani, Lorenzo, He, Jiawei, Negroni, Elisa, Sakai, Hiroshi, Law, Yiu Chun, Siu, Men Yi Mona, Wan, Raymond, Corneau, Aurélien, Tajbakhsh, Shahragim, Cheung, Hiu Tung, and Le grand, Fabien
Abstract: Adult tissue repair and regeneration require stem-progenitor cells that can self-renew and generate differentiated progeny. Skeletal muscle regenerative capacity relies on muscle satellite cells (MuSCs)and their interplay with different cell types within the niche. However, our understanding of skeletal muscle tissue cellular composition is limited. Here, using a combined approach of single-cell RNA sequencing and mass cytometry, we precisely mapped 10 different mononuclear cell types in adult mouse muscle. We also characterized gene signatures and determined key discriminating markers of each cell type. We identified two previously understudied cell populations in the interstitial compartment. One expresses the transcription factor scleraxis and generated tenocytes in vitro. The second expresses markers of smooth muscle and mesenchymal cells (SMMCs)and, while distinct from MuSCs, exhibited myogenic potential and promoted MuSC engraftment following transplantation. The blueprint presented here yields crucial insights into muscle-resident cell-type identities and can be exploited to study muscle diseases.
Published: 2019

40. Les nouvelles approches de l'analyse multi-paramétrique en cytométrie de masse : caractérisation des cellules réservoirs du VIH

Author: Corneau, Aurélien, Cytométrie Pitié-Salpêtrière (PASS-CYPS), Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé (PASS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), PSL Research University, Brigitte Autran, and Véronique Frachet
Subjects: Marqueurs d’épuisement cellulaire, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, HIV, Activation, VIH, Immune-checkpoint, Cycle cellulaire, Analyse multiparamétrique, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Multiparametric analysis, Cytométrie de masse, Cellular activation, [SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology, Cell cyle, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Mass cytometry
Abstract: Mass Cytometry (MCM) has revolutionized the study of cell and phenotypic diversity, significantly increasing the number of markers that can be analyzed simultaneously (41 to date). By making it possible to precisely define the status of lymphocyte populations, particularly with regard to their differentiation, activation and entry into the cell cycle, CMM has uncovered small subsets previously unknown. In this study, MMC was used to try to better characterize HIV reservoirs. With the introduction of combination antiretroviral therapy (ART) in 1996, HIV infection has been transformed from a fatal fate to a manageable chronic disease with a normal lifespan due to a reduction in active viral replication (the amount of virus is below optimal detection limits). However, if treatment is interrupted, the patient's viral load increases again due to viable provirus reservoirs located in long-lived cell populations that cannot be eliminated by current therapies. These infected reservoir cells are a major obstacle to the eradication of HIV. The best characterized reservoir is that of CD4+ T lymphocytes and is mainly hosted in MCT, MST, MSCT, and Tfh. An initial study allowed us to evaluate the stages of the cell cycle in association with markers of differentiation, activation and depletion, leading to an in-depth evaluation of the quiescence status of CD4 T cells likely to harbour latent HIV reservoirs. This broad multiplex analysis demonstrates that certain subsets of LTCD4+CD25-HLA-DR - classically considered "at rest" - actually contain significant amounts of cells cycling or expressing inhibitory receptors, opening new avenues for redefining quiescent CD4 T cells in peripheral blood. A second study aimed to define in vivo HIV-producing CD4 LT populations. We developed a multiparametric analysis on cells from HIV+ patients on ART and in the therapeutic interruption phase (TIA). This study shows that CD3+CD4+CD32high cells express a high level of activation markers and receive important activation signals via cytokines, in contrast to CD32a cells. On the other hand, the analysis of HIV-producing LTCD4+ (expressing the p24 capsid protein), allowed us to detect a very low number of p24+ positive cells (less than 0.004% in ATI phase but none before). The phenotype of the producing cells was then highlighted. They are T lymphocytes that do not express CD8, enriched with a factor 4 in TSCM cells and a factor 2 in TFH. These populations are highly enriched in activated cells co-expressing 3 activation markers (increased by a factor of 20) and are cycling (Ki67+) and/or over-expressing immune control molecules (ICP) with an enrichment of a factor of 500. This allows us to detect producer cells with much higher frequencies in these TCD3+CD8- populations in cycle up to 0.08%, and in G2 phase (2.46%), but also in cells showing poly-expression of the 4 immune-checkpoints (2.27%). The advent of mass cytometry has exponentially increased the information we could obtain on a cell. Thanks to this tool, the identification of the cell cycle, in correlation with different phenotypic markers, makes it possible to explore previously inaccessible information, including the analysis of the latent and productive reservoirs of HIV. This work thus makes it possible to characterize as precisely as possible these HIV-producing cells, but also latent cells, and potential reservoirs of the virus.; La cytométrie de masse CMM) a révolutionné l'étude de la diversité cellulaire et phénotypique, en augmentant de manière significative le nombre de marqueurs pouvant être analysés simultanément (41 à ce jour). En permettant de définir précisément l'état des populations de lymphocytes, notamment en ce qui concerne leur différenciation, activation et leur entrée dans le cycle cellulaire, la CMM a mis au jour de petits sous-ensembles jusqu'ici inconnus. Dans cette étude, la CMM a été utilisée pour tenter de mieux caractériser les réservoirs du VIH. Avec l'introduction de la thérapie antirétrovirale combinée (ART) en 1996, l'infection par le VIH est passée d'un destin fatal à une maladie chronique gérable avec une durée de vie normale grâce à une réduction de la réplication virale active (la quantité de virus est en deçà des limites de détection optimales). Cependant, si le traitement est interrompu, la charge virale chez le patient augmente à nouveau du fait des réservoirs de provirus viables localisés dans des populations de cellules à longue durée de vie et qui ne peuvent pas être éliminées par les traitements actuels. Ces cellules infectées réservoirs constituent un obstacle majeur à l'éradication du VIH. Le réservoir le mieux caractérisé est celui des lymphocytes T CD4+ et est principalement hébergé dans les TCM, les TTM, les TSCM et les Tfh. Une première étude nous a permis d’évaluer les stades du cycle cellulaire en association à des marqueurs de différenciation, d'activation et d'épuisement, pour aboutir à une évaluation poussée de l'état de quiescence des lymphocytes T CD4 susceptibles d’abriter les réservoirs latents de VIH. Cette large analyse multiplexe démontre que certains sous-ensembles des LTCD4+CD25-HLA-DR- classiquement considérés "au repos"- contiennent en fait des quantités notables de cellules en cycle ou exprimant des récepteurs inhibiteurs, ouvrant de nouvelles voies pour une redéfinition des cellules T CD4 quiescentes du sang périphérique. Une deuxième étude avait pour but de définir les populations de LT CD4 produisant du VIH in vivo. Nous avons développé une analyse multiparamétrique sur des cellules de patients VIH+ sous ART et en phase d’interruption thérapeutique (ATI). Cette étude met en évidence que les cellules CD3+CD4+CD32high expriment un fort taux de marqueurs d’activation et reçoivent d’importants signaux d’activation via des cytokines, à l'inverse des cellules CD32a-. D'autre part, l'analyse des LTCD4+ producteurs de VIH (exprimant la protéine de capside p24), nous a permis de détecter un très faible nombre de cellules positives p24+ (inférieur à 0,004% en phase d’ATI mais aucun avant). Le phénotype des cellules productrices a ensuite été mis en évidence. Il s’agit de lymphocytes T n’exprimant pas de CD8, enrichis d’un facteur 4 en cellules TSCM, et d'un facteur 2 en TFH. Ces populations sont très enrichies en cellules activées co-exprimant 3 marqueurs d’activation (augmentés d’un facteur 20) et sont en cycle (Ki67+) et/ou sur-expriment des molécules de contrôle immunitaire (ICP) avec un enrichissement d’un facteur 500. Ceci nous permet de détecter des cellules productrices avec des fréquences beaucoup plus élevées dans ces populations TCD3+CD8- en cycle à hauteur de 0,08%, et en phase G2 (2,46%), mais également dans les cellules présentant une poly-expression des 4 immune-checkpoints (2,27%). L’avènement de la cytométrie de masse a augmenté de façon exponentielle les informations que nous pouvions obtenir sur une cellule. Grâce à cet outil, l’identification du cycle cellulaire, en corrélation avec différents marqueurs phénotypiques, permet d’explorer des informations jusque-là inaccessibles, entre autre l’analyse des réservoirs latents et productif du VIH. Ce travail permet ainsi de caractériser le plus précisément possible ces cellules productrices de VIH, mais aussi les cellules latentes, et potentiellement réservoirs du virus.
Published: 2018

41. Que la lumière soit. Et si ce n’était plus seulement vrai !

Author: Idziorek, Thierry, Cazareth, Julie, Blanc, Catherine, Jouy, Nathalie, Bourdely, Pierre, CORNEAU, Aurélien, Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U1172 Inserm - U837 (JPArc), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Cytométrie Pitié-Salpêtrière (PASS-CYPS), Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé (PASS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratory of Development and Plasticity of the Neuroendocrine Brain [Lille], Centre de recherche Jean-Pierre Aubert-Neurosciences et Cancer, Université de Lille, Droit et Santé-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Université Côte d'Azur (UCA), Plateforme Cytométrie Pitié-Salpêtrière (LUMIC-CYPS), Unité Mixte de Service d'Imagerie et de Cytométrie (UMS LUMIC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Unité Mixte de Service d'Imagerie et de Cytométrie (UMS LUMIC), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837 (JPArc), Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Institut des Maladies Emergentes et des Thérapies Innovantes (IMETI), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), BLANC, Catherine, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé, Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS, [SDV.BIO] Life Sciences [q-bio]/Biotechnology
Abstract: International audience; The last decade has been an era of accelerated technological progress for flow cytometry. New technologies have been developed such as mass cytometry in which standard fluorochromes have been replaced by lanthanide-based non-radioactive metals and by spectral cytometry that measures the complete fluorescence spectrum. In this review, we schematically describe conventional, mass and spectral cytometry and present the plus and minus of each technology.
Published: 2018

42. [Fiat Lux. May be no more true in cytometry! Go to mass and spectrum but still stay classic]

Author: Idziorek, Thierry, Cazareth, Julie, Blanc, Catherine, Jouy, Nathalie, Bourdely, Pierre, Corneau, Aurélien, Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U1172 Inserm - U837 (JPArc), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Lille Nord de France (COMUE)-Université de Lille, Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Service d'Imagerie et de Cytométrie (UMS LUMIC), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Cytométrie Pitié-Salpêtrière (PASS-CYPS), Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé (PASS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratory of Development and Plasticity of the Neuroendocrine Brain [Lille], Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Lille Nord de France (COMUE)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Lille Nord de France (COMUE)-Université de Lille, Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Institut des Maladies Emergentes et des Thérapies Innovantes (IMETI), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837 (JPArc), Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Université Nice Sophia Antipolis (1965 - 2019) (UNS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Light, Spectrum Analysis, Animals, Humans, Flow Cytometry, Fluorescence, Mass Spectrometry, Fluorescent Dyes
Abstract: International audience; The last decade has been an era of accelerated technological progress for flow cytometry. New technologies have been developed such as mass cytometry in which standard fluorochromes have been replaced by lanthanide-based non-radioactive metals and by spectral cytometry that measures the complete fluorescence spectrum. In this review, we schematically describe conventional, mass and spectral cytometry and present the plus and minus of each technology.
Published: 2018

43. High-Dimensional Single-Cell Cartography Reveals Novel Skeletal Muscle-Resident Cell Populations

Author: Giordani, Lorenzo, primary, He, Gary J., additional, Negroni, Elisa, additional, Sakai, Hiroshi, additional, Law, Justin Y.C., additional, Siu, M. Mona, additional, Wan, Raymond, additional, Corneau, Aurélien, additional, Tajbakhsh, Shahragim, additional, Cheung, Tom H., additional, and Le Grand, Fabien, additional
Published: 2019
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44. A novel combination of chemotherapy and immunotherapy controls tumor growth in mice with a human immune system

Author: Burlion, Aude, primary, Ramos, Rodrigo N., additional, KC, Pukar, additional, Sendeyo, Kélhia, additional, Corneau, Aurélien, additional, Ménétrier-Caux, Christine, additional, Piaggio, Eliane, additional, Olive, Daniel, additional, Caux, Christophe, additional, and Marodon, Gilles, additional
Published: 2019
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45. CD8+T-bet+ cells as a predominant biomarker for inclusion body myositis

Author: Dzangué-Tchoupou, Gaëlle, primary, Mariampillai, Kuberaka, additional, Bolko, Loïs, additional, Amelin, Damien, additional, Mauhin, Wladimir, additional, Corneau, Aurélien, additional, Blanc, Catherine, additional, Allenbach, Yves, additional, and Benveniste, Olivier, additional
Published: 2019
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46. Comprehensive Mass Cytometry Analysis of Cell Cycle, Activation, and Coinhibitory Receptors Expression in CD4 T Cells from Healthy and HIV-Infected Individuals

Author: Corneau, Aurélien, Cosma, Antonio, Even, Sophie, Katlama, Christine, Le Grand, Roger, Frachet, Veronique, Blanc, Catherine, Autran, Brigitte, Centre d'Immunologie et de Maladies Infectieuses (CIMI), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Ecole Doctorale 472 : Systèmes Intégrés, Environnement et Biodiversité (EPHE) (ED 472 SIEB), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Plateforme Cytométrie Pitié-Salpêtrière (LUMIC-CYPS), Unité Mixte de Service d'Imagerie et de Cytométrie [CHU Saint-Antoine] (UMS LUMIC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Immunologie des Maladies Virales et Autoimmunes (IMVA - U1184), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service des maladies infectieuses et tropicales [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Unité Mixte de Service d'Imagerie et de Cytométrie (UMS LUMIC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Service d'Immunologie [CHU Pitié-Salpétrière], Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé (PASS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Ecole Doctorale 472 mention « Systèmes intégrés, environnement et biodiversité » (SIEB) (ED 472 SIEB), École Pratique des Hautes Études (EPHE), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Unité Mixte de Service d'Imagerie et de Cytométrie [CHU Saint-Antoine] (UMS LUMIC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de Maladies Infectieuses et Tropicales [CHU Pitié-Salpêtrière], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2017

47. Analysis of cell surface and intranuclear markers on non-stimulated human PBMC using mass cytometry

Author: Dzangué-Tchoupou, Gaëlle, primary, Corneau, Aurélien, additional, Blanc, Catherine, additional, Benveniste, Olivier, additional, and Allenbach, Yves, additional
Published: 2018
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48. High-Dimensional Single-Cell Cartography Reveals Novel Skeletal Muscle Resident Cell Populations

Author: Giordani, Lorenzo, primary, He, Gary J., additional, Negroni, Elisa, additional, Sakai, Hiroshi, additional, Law, Justin Y. C., additional, Siu, M. Mona, additional, Wan, Raymond, additional, Corneau, Aurélien, additional, Tajbakhsh, Shahragim, additional, Cheung, Tom H., additional, and Le Grand, Fabien, additional
Published: 2018
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49. A novel combination of chemotherapy and immunotherapy controls tumor growth in mice with a human immune system

Author: Burlion, Aude, primary, Ramos, Rodrigo N., additional, Pukar, KC, additional, Sendeyo, Kélhia, additional, Corneau, Aurélien, additional, Ménétrier-Caux, Christine, additional, Piaggio, Eliane, additional, Olive, Daniel, additional, Caux, Christophe, additional, and Marodon, Gilles, additional
Published: 2017
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50. Evaluating the Efficiency of Isotope Transmission for Improved Panel Design and a Comparison of the Detection Sensitivities of Mass Cytometer Instruments

Author: Tricot, Sabine, Meyrand, Mickael, Sammicheli, Chiara, Elhmouzi-Younes, Jamila, Corneau, Aurélien, Bertholet, Sylvie, Malissen, Marie, Le Grand, Roger, Nuti, Sandra, Luche, Hervé, Cosma, Antonio, Plateforme Cytométrie Pitié-Salpêtrière (LUMIC-CYPS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Unité Mixte de Service d'Imagerie et de Cytométrie [CHU Saint-Antoine] (UMS LUMIC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Immunologie des Maladies Virales et Autoimmunes (IMVA - U1184), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'Immunophénomique (CIPHE), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), EU/EFPIA Innovative Medicines Initiative Joint Undertaking [115308], French Government Program: ``Investissement d'avenir' [ANR-10-EQPX-02-01], Unité Mixte de Service d'Imagerie et de Cytométrie [CHU Saint-Antoine] (UMS LUMIC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), and Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
Subjects: standardization, Key terms: mass cytometry, flow cytometry, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], CyTOF
Abstract: International audience; The recent introduction of mass cytometry, a technique coupling a cell introduction system generating a stream of single cells with mass spectrometry, has greatly increased the number of parameters that can be measured per single cell. As with all new technology there is a need for dissemination of standardization and quality control procedures. Here, we characterize variations in sensitivity observed across the mass range of a mass cytometer, using different lanthanide tags. We observed a five-fold difference in lanthanide detection over the mass range and demonstrated that each instrument has its own sensitivity pattern. Therefore, the selection of lanthanide combinations is a key step in the establishment of a staining panel for mass cytometry-based experiments, particularly for multicenter studies. We propose the sensitivity pattern as the basis for panel design, instrument standardization and future implementation of normalization algorithms.
Published: 2015

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