1. Anti-Neuroinflammatory and Anti-Inflammatory Activities of Phenylheptatriyne Isolated from the Flowers of Coreopsis lanceolata L. via NF-κB Inhibition and HO-1 Expression in BV2 and RAW264.7 Cells
- Author
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Linsha Dong, Zhiming Liu, Hwan Lee, Wonmin Ko, Dong-Sung Lee, Eun-Rhan Woo, and Chi-Su Yoon
- Subjects
Lipopolysaccharides ,Anti-Inflammatory Agents ,Nitric Oxide Synthase Type II ,medicine.disease_cause ,Coreopsis lanceolata L ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,030212 general & internal medicine ,Biology (General) ,Spectroscopy ,0303 health sciences ,biology ,Interleukin ,General Medicine ,mouse macrophage RAW 264.7 ,Computer Science Applications ,flower ,Chemistry ,Coreopsis lanceolata ,Tumor necrosis factor alpha ,Microglia ,Coreopsis ,Signal Transduction ,medicine.drug_class ,QH301-705.5 ,Flowers ,Nitric Oxide ,Catalysis ,Anti-inflammatory ,Article ,Dinoprostone ,Nitric oxide ,Proinflammatory cytokine ,Inorganic Chemistry ,03 medical and health sciences ,medicine ,Animals ,Physical and Theoretical Chemistry ,Molecular Biology ,QD1-999 ,030304 developmental biology ,Inflammation ,Plant Extracts ,Macrophages ,Organic Chemistry ,biology.organism_classification ,Molecular biology ,anti-inflammation ,Leptosidin ,RAW 264.7 Cells ,chemistry ,Oxidative stress ,Heme Oxygenase-1 ,mouse microglia BV2 - Abstract
Aging is associated with immune disregulation and oxidative stress which lead to inflammation and neurodegenerative diseases. We have tried to identify the anti-neuroinflammatory and anti-inflammatory components of Coreopsis lanceolata L. The dried flowers of C. lanceolata were extracted with 70% EtOH, and the obtained extract was divided into CH2Cl2, EtOAc, n-BuOH, and H2O fractions. The CH2Cl2 fraction was separated using silica gel and C-18 column chromatography to yield phenylheptatriyne (1), 2′-hydroxy-3,4,4′-trimethoxychalcone (2), and 4′,7-dimethoxyflavanone (3). Additionally, the EtOAc fraction was subjected to silica gel, C-18, and Sephadex LH-20 column chromatography to yield 8-methoxybutin (4) and leptosidin (5). All the compounds isolated from C. lanceolata inhibited the production of nitric oxide (NO) in LPS-induced BV2 and RAW264.7 cells. In addition, phenylheptatriyne and 4′,7-dimethoxyflavanone reduced the secretion of inflammatory cytokines, tumor necrosis factor alpha (TNF-α), and interleukin (IL)-6. Among them, phenylheptatriyne was significantly downregulated in the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). Subsequently, phenylheptatriyne also effectively inhibited nuclear factor-kappa B (NF-κB) activation in LPS-stimulated BV2 and RAW264.7 cells. Based on these results, the anti-neuroinflammatory effect of phenylheptatriyne isolated from C. lanceolata was confirmed, which may exert a therapeutic effect in treatment of neuroinflammation-related diseases.
- Published
- 2021