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Information on Influenza A virus (IAV) prevalence in shorebird populations in Guatemala is scarce, and no live captures of shorebirds have been made for this purpose. The only reported case of Influenza A in shorebirds in Guatemala corresponded to a harvested individual. To update and obtain information of IAV in shorebirds, a transversal study was made on the South Coast of Guatemala. The main objective of this study was to evaluate presence of Influenza A among migratory shorebirds in stopover areas in the country. Captures and sampling took place in three sites during migration (October–April) over three migratory periods (2016–2017, 2017–2018, and 2018–2019). In total, 127 shorebirds belonging to 14 species of genera Calidris, Charadrius, Tringa, and Actitis were captured using mist nets. Tracheal and cloacal swabs were collected from each bird using a cotton swab, then tested with RT-PCR techniques targeting Influenza A. Blood samples were taken from the jugular or basilic vein to obtain serum that later was tested using a commercial ELISA Kit for detection of antibodies against Influenza A. Birds were released after sampling. All samples were PCR negative (0/127).One sandpiper specimen (Calidris minutilla) tested sero-positive to Influenza A through the ELISA test (1/122). These results suggest that IAV infection in this region may be minimal, as other studies have reported; nevertheless, it is important to consider that sample size was small in this study, and that the species included may not be the best indicators. [ABSTRACT FROM AUTHOR]

Copyright of Revista de la Universidad del Valle de Guatemala is the property of Universidad del Valle de Guatemala and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista de la Universidad del Valle de Guatemala is the property of Universidad del Valle de Guatemala and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Avian Diseases is the property of American Association of Avian Pathologists, Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista de la Universidad del Valle de Guatemala is the property of Universidad del Valle de Guatemala and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The agent of Chagas disease, Trypanosoma cruzi, is phylogenetically divided into two lineages, T. cruzi I (TCI) and II (TCII). TCI is found in sylvatic and domestic habitats across South America. Despite a high prevalence of TCII in domestic habitats in South America, it has been rarely found in domestic habitats in Central America and Mexico. This may be caused by limitations in detection tests previously used. A modified hemi-nested polymerase chain reaction assay was developed to improve detection of TCI and TCII mini-exon genes. This method detected TCI and II concurrently in 84% of 44 cultured isolates from Triatoma dimidiata specimens collected inside homes across the disease-endemic area in Guatemala.