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21 results on '"Corbett, Carlos E.P."'

5. Protocol for DNA extraction of Cryptosporidium spp. oocysts in fecal samples

Author

Gonçalves, Elenice M.N., Araújo, Ronalda S., Orban, Magali, Matté, Glavur R., Matté, Maria Helena, and Corbett, Carlos E.P.

Subjects
Molecular techniques, Diagnosis, Cryptosporidium spp
Abstract

Molecular characterization of Cryptosporidium spp.oocysts in clinical samples is useful for public health since it allows the study of sources of contamination as well as the transmission in different geographical regions. Although widely used in developed countries, in Brazil it is restricted to academic studies, mostly using commercial kits for the extraction of genomic DNA, or in collaboration with external reference centers, rendering the method expensive and limited. The study proposes the application of the modifications recently introduced in the method improving feasibility with lower cost. This method was efficient for clinical samples preserved at -20 °C for up to six years and the low number of oocysts may be overcomed by repetitions of extraction. A caracterização molecular de oocistos de Cryptosporidium spp. em amostras clínicas é útil à saúde pública, pois permite estudo das fontes de contaminação e a transmissão em determinadas regiões geográficas. Apesar de largamente utilizada em países desenvolvidos, no Brasil está restrita aos estudos acadêmicos, na maioria utilizando kits comerciais para extração do DNA genômico, ou em colaborações com centros de referência externos, o que torna o método caro e limitado. Este estudo propõe a introdução de modificações nos métodos existentes para melhorar a viabilidade e baixar custos. O método proposto foi eficiente em amostras clínicas preservadas a -20 °C por até seis anos e o baixo número de oocistos pode ser contornado por replicadas extrações de DNA.

Published
2008

7. Canine visceral leishmaniasis due to Leishmania (L.) infantum chagasi in Amazonian Brazil: comparison of the parasite density from the skin, lymph node and visceral tissues between symptomatic and asymptomatic, seropositive dogs

Author

Lima, Luciana Vieira R., primary, Carneiro, Liliane Almeida, additional, Campos, Marliane Batista, additional, Chagas, Eujênia Janis, additional, Laurenti, Márcia D., additional, Corbett, Carlos E.P., additional, Lainson, Ralph, additional, and Silveira, Fernando Tobias, additional

Published
2010
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20. Insulin-like Growth Factor (IGF)-I affects parasite growth and host cell migration in experimental cutaneous leishmaniasis.

Author

Gomes, ClÁudia Maria De C., Goto, Hiro, Ribeiro Da Matta, Vania LÚcia, Laurenti, MÁrcia Dalastra, Gidlund, Magnus, and Corbett, Carlos E.P.

Subjects
SOMATOMEDIN, LEISHMANIASIS, HOST-parasite relationships, PHYSIOLOGY
Abstract

While the control or progression of leishmaniasis depends on host immune responses, the initial inflammatory process represents a key event. This process involves the participation of several cytokines and growth factors induced during inflammation as well as factors already present at the site of infection such as insulin-like growth factor (IGF)-I. We have previously demonstrated a potential role for IGF-I in experimental cutaneous leishmaniasis based on the significant increase in lesion size seen in mice injected with Leishmania promastigotes preactivated with IGF-I. In the present study we show that preactivation of Leishmania (Leishmania) amazon-ensis promastigotes with IGF-I induces an increase in the actual number of parasites at the lesion site from seven days postinfection, in addition to a more intense inflammatory infiltrate. There was a higher numerical density of polymorphonuclear neutrophils from 3 to 24 h, and of mononuclear cells from 48 h of infection onward. A higher density of polymorphonuclear neutrophils and mononuclear cells harboring parasites was also observed. The most important observation, however, was that more parasites per cell were present, revealing that IGF-I appears to favour parasite growth within the macro-phages. These results strongly suggest an important role for IGF-I in the development of cutaneous leishmaniasis, where it influences both the inflammatory process and parasite growth. [ABSTRACT FROM AUTHOR]

Published
2000
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21. Does CO2 Pneumoperitoneum Alter the Ultra-Structuture of the Mesothelium? 1 [1] This research was funded by FAPESP—02/07886-0.

Author

Rosário, Marcos T.A., Ribeiro, Ulysses, Corbett, Carlos E.P., Ozaki, Alberto C., Bresciani, Cláudio C., Zilberstein, Bruno, and Gama-Rodrigues, Joaquim J.

Subjects
*ABDOMINAL surgery, *SURGERY, *LAPAROSCOPY, *ENDOSCOPY, *LAPAROSCOPIC surgery, *ARTIFICIAL pneumoperitoneum, *MEDICAL research
Abstract

Background: Pneumoperitoneum may be responsible for ultra-structural alterations in the mesothelium during laparoscopy. To characterize the effect of pneumoperitoneum on the mesothelial cells with CO2 and compressed air; and to compare to laparotomy and control group (anesthesia only). Materials and methods: Forty C-57 mice were divided in four groups of 10 animals each: CO2, air, laparotomy, and control group. The animals were submitted to pneumoperitoneum at 8 mmHg during 30 min (CO2 or compressed air). Five animals of each group were sacrificed 2 and 24 h after the procedure. Fragments of parietal peritoneum were collected and processed for scanning electron microscopy. Results: Control group revealed uninterrupted mesothelial cells, without any evidence of cellular limits; close contact between the cells; absence of intercellular clefts and presence of microvilli. In the laparotomy group, similar results to the control group, with decreased microvilli were noted. Air pneumoperitoneum was associated with alterations in the morphology of the mesothelial cells, clear cellular limits, and cells with spherical and fusiforme formats. CO2 pneumoperitoneum showed mesothelial cells with clear cellular limits, predominantly spherical cellular format, and intercellular clefts that allowed the visualization of the exposed basal membrane. These alterations were more intense after 24 h. There was a statistical significance between CO2 group (2 and 24 h) compared to the control group and laparotomy for cellular limits, intercellular clefts and microvilli, P < 0.0001. Conclusions: Pneumoperitoneum causes damage in the mesothelial ultra-structure, which differs from the laparotomy group. CO2 pneumoperitoneum is more harmful to the mesothelium than the air. [Copyright &y& Elsevier]

Published
2006
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