1. Roles of the tubulin-based cytoskeleton in the Toxoplasma gondii apical complex.
- Author
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Tell i Puig, Albert and Soldati-Favre, Dominique
- Subjects
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TOXOPLASMA gondii , *TUBULINS , *CYTOSKELETON , *CELL physiology , *MICROTUBULES , *REVERSE genetics - Abstract
The subpellicular microtubules (SPMTs) shape the parasite, contributing to its motility and adaptation across various environments. They possess a high density of microtubule (MT)-associated proteins, providing exceptional stability. The conoid consists of tubulin fibers uniquely shaped by MT-associated proteins. Conoid extrusion through the apical polar ring regulates parasite motility by acting as a gatekeeper, controlling F-actin access to the intrapellicular space. The intraconoidal MTs (ICMTs) play a pivotal role in positioning the rhoptries, facilitating their iterative discharge to enable successful invasion and subversion of host cellular functions. The role of kinesins and dyneins in trafficking and MT dynamics remains understudied in Toxoplasma gondii , a parasite that also has a yet-to-be-deciphered tubulin code, which defines MT properties. Microtubules (MTs) play a vital role as key components of the eukaryotic cytoskeleton. The phylum Apicomplexa comprises eukaryotic unicellular parasitic organisms defined by the presence of an apical complex which consists of specialized secretory organelles and tubulin-based cytoskeletal elements. One apicomplexan parasite, Toxoplasma gondii , is an omnipresent opportunistic pathogen with significant medical and veterinary implications. To ensure successful infection and widespread dissemination, T. gondii heavily relies on the tubulin structures present in the apical complex. Recent advances in high-resolution imaging, coupled with reverse genetics, have offered deeper insights into the composition, functionality, and dynamics of these tubulin-based structures. The apicomplexan tubulins differ from those of their mammalian hosts, endowing them with unique attributes and susceptibility to specific classes of inhibitory compounds. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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