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Your search keyword '"Connally, Russell E."' showing total 12 results
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12 results on '"Connally, Russell E."'

5. Sensitive Time-Gated Immunoluminescence Detection of Prostate Cancer Cells Using a TEGylated Europium Ligand

Author

Sayyadi, Nima, primary, Justiniano, Irene, additional, Connally, Russell E., additional, Zhang, Run, additional, Shi, Bingyang, additional, Kautto, Liisa, additional, Everest-Dass, Arun V., additional, Yuan, Jingli, additional, Walsh, Bradley J., additional, Jin, Dayong, additional, Willows, Robert D., additional, Piper, James A., additional, and Packer, Nicolle H., additional

Published
2016
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11. In Silico Evaluation and Testing of Fluorescence In Situ Hybridization 16S rRNA Probes for Staphylococcus aureus.

Author

Lawson, Thomas S., Connally, Russell E., Vemulpad, Subramanyam, and Piper, James A.

Subjects
*FLUORESCENCE in situ hybridization, *COMPARATIVE studies, *COMPUTER software, *CONFIDENCE intervals, *DATABASES, *DNA probes, *HEALTH, *PROBABILITY theory, *RESEARCH funding, *STAPHYLOCOCCUS aureus, *STATISTICAL hypothesis testing, *T-test (Statistics), *WORLD Wide Web, *IN vitro studies
Abstract

Background: Staphylococcus aureus is a clinically important pathogen. A small number of whole-cell fluorescence in situ hybridization (FISH) probes have been reported to detect S. aureus. New online computational tools for in silico design and testing make it possible to assess candidate FISH probes for S. aureus.Materials and Methods: Six online tools, NCBI-Nucleotide, Ribosomal Database Project, NCBI-Blast, Reverse-Complement, Probecheck, and mathFISH, were employed in a workflow to evaluate FISH probes for S. aureus. A previously reported probe, Staaur-16S69, was compared to a new probe, KT18-16S68, predicted by mathFISH to have the same performance.Results: A number of new probes for S. aureus were predicted to perform as well or better in silico as those previously reported. When tested in a FISH assay, Staaur and a new probe, KT18, were found to have the same performance.Conclusion: Existing and new FISH probes for S. aureus were found to be accurately identified and characterized with online computational tools. In silico evaluation of probes has the potential to reduce the time spent evaluating probes in the laboratory. [ABSTRACT FROM AUTHOR]

Published
2011
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12. Express fluorescence in situ hybridization methods for the detection of Staphylococcus aureus.

Author

Lawson TS, Connally RE, Vemulpad S, and Piper JA

Subjects
DNA, Bacterial analysis, High-Throughput Screening Assays, Humans, Staphylococcal Infections microbiology, Staphylococcus aureus cytology, Staphylococcus aureus genetics, Staphylococcus epidermidis genetics, Staphylococcus epidermidis isolation & purification, Time Factors, In Situ Hybridization, Fluorescence methods, Staphylococcal Infections diagnosis, Staphylococcus aureus isolation & purification
Abstract

Background: As a proof-of-concept, the feasibility of detecting Staphylococcus aureus faster than previous whole-cell fluorescent in situ hybridization (FISH) methods was tested., Methods: Isolates of Staphylococcus were treated with three rapid slide-based FISH protocols and DNA probes. Protocols were shortened by optimizing, combining or omitting steps., Results: All FISH protocols detected S. aureus and not the phenotypically similar Staphylococcus epidermidis. The express FISH assay was completed in 24 minutes. The one-step FISH assay with NaCl and the one-step with phosphate buffered saline (PBS) assay took 19 minutes, but yielded a weaker signal., Conclusions: The exploratory study identified S. aureus two to three times faster than previous methods. Additional testing in a clinical laboratory scenario (for example with positive blood-culture bottles) is warranted.

Published
2011

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