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Abstract To date, earlier diagnosis of Alzheimer’s disease (AD) is still challenging. Recent studies revealed the elevated expression of connective tissue growth factor (CTGF) in AD brain is an upstream regulator of amyloid-beta (Aβ) plaque, thus CTGF could be an earlier diagnostic biomarker of AD than Aβ plaque. Herein, we develop a peptide-coated gold nanocluster that specifically targets CTGF with high affinity (KD ~ 21.9 nM). The probe can well penetrate the blood-brain-barrier (BBB) of APP/PS1 transgenic mice at early-stage (earlier than 3-month-old) in vivo, allowing non-invasive NIR-II imaging of CTGF when there is no appearance of Aβ plaque deposition. Notably, this probe can also be applied to measuring CTGF on postmortem brain sections by multimodal analysis, including fluorescence imaging, peroxidase-like chromogenic imaging, and ICP-MS quantitation, which enables distinguishment between the brains of AD patients and healthy people. This probe possesses great potential for precise diagnosis of earlier AD before Aβ plaque formation.

The treatment of diabetic wounds is a major challenge faced by the medical system, and there is a growing interest in developing innovative therapies to accelerate wound healing. Regenerative medicine with cells has shown promising potential in skin repair, with the regenerative properties primarily attributed to the paracrine effects of secreted products, including exosomes. Compared to cell-based approaches, using exosomes as a cell-free therapy for chronic wounds has several advantages. Exosomes can regulate intercellular communication by releasing their contents, including mRNA, miRNA, lipids, and proteins, which further promote wound healing. Exosomes are well explored in biomedical application owing to their advantages such as their biocompatibility and low immunogenicity. However, the common method of exosome administration is through injection, but due to their rapid clearance rate in the body, maintaining the necessary therapeutic concentration around the wound is challenging. Therefore, it is necessary to develop a new biocompatible scaffold as a carrier for extracellular vesicles, allowing them to sustain the therapeutic concentration at chronic non-healing wound sites and continuously promote wound healing. Engineered exosomes are kinds of exosomes modified with internal treated molecules, surface decoration or delivered through engineered platform. In addition, some researchers have further processed and modified exosomes, known as engineered exosomes, with internal treated molecules, surface decoration, or delivery through engineered platforms. Compared to regular exosomes, engineered exosomes have greater advantages in promoting wound healing. In this review, we summarize the molecular mechanisms of exosomes from different sources with varying modifications in wound healing. Advantages and limitations of different engineered exosomes for chronic wound repair were also discussed. Finally, we highlight the challenges and future development directions for translating our knowledge of engineered exosomes into clinical practice.

Abstract The morbidity and mortality of gastrointestinal tumours remain high worldwide. Surgical resection is currently the most critical radical therapeutic schedule, while postoperative complications and sentinel lymph node (SLN) identification are closely related to the outcome. Indocyanine green (ICG)-mediated fluorescence imaging is increasingly being used in gastrointestinal surgery. It has been embraced by various surgical disciplines as a potential method to improve lymph node detection and enhance surgical field visualization. ICG can passively concentrate in SLN because of enhanced permeation and retention effects. After excitation by near-infrared light devices, SLN can display higher intensity fluorescence, helping visualization for better lymph node dissection. In addition, visual assessment of intestinal blood flow through ICG may reduce the incidence of anastomotic leakage. Although it has good clinical application, ICG-imaging still faces some problems, such as a higher false-negative rate, poorly targeted biodistribution, and lower fluorescence contrast, due to the lack of active tumour targeting. Thus, different ICG-coupled nanoparticles with inherent characteristics or functional modification-enhanced SLN identification features for gastrointestinal cancers bring benefit through active tumour targeting, superior tumour-background ratio, and high resolution. Nano-ICG combined with potential substances, including enhanced imaging contrast and/or combination therapy (chemotherapy, targeted therapy, immunotherapy, etc.), have been packaged and accumulated in the tumour area through active targeting for multimodal imaging and treatment. In this review, we outline the intraoperative application and possible future nanodirections of ICG in gastrointestinal cancer. The prospects and challenges of nano-ICG diagnostic and therapeutic methods in clinical applications are also discussed. Graphical Abstract

Introduction Anastomotic leakage (AL) is defined as the failure of complete healing or disruption of the anastomosis subsequent to rectal cancer surgery, resulting in the extravasation of intestinal contents into the intra-abdominal or pelvic cavity. It is a serious complication of rectal cancer surgery, accounting for a considerable increase in morbidity and mortality. The use of fluorescence imaging technology in surgery allows surgeons to better evaluate blood perfusion. However, the conclusions of some existing studies are not consistent, so a consensus on whether the near-infrared indocyanine green (NIR–ICG) imaging system can reduce the incidence of AL is needed.Methods This POSTER trial is designed as a multicentre, prospective, randomised controlled clinical study adhering to the “population, interventions, comparisons, outcomes (PICO)” principles. It is scheduled to take place from August 2019 to December 2024 across eight esteemed hospitals in China. The target population consists of patients diagnosed with rectal cancer through pathological confirmation, with tumours located≤10 cm from the anal verge, eligible for laparoscopic surgery. Enrolled patients will be randomly assigned to either the intervention group or the control group. The intervention group will receive intravenous injections of ICG twice, with intraoperative assessment of anastomotic blood flow using the near-infrared NIR–ICG system during total mesorectal excision (TME) surgery. Conversely, the control group will undergo conventional TME surgery without the use of the NIR-ICG system. A 30-day follow-up period postoperation will be conducted to monitor and evaluate occurrences of AL. The primary endpoint of this study is the incidence of AL within 30 days postsurgery in both groups. The primary outcome investigators will be blinded to the application of ICG angiography. Based on prior literature, we hypothesise an AL rate of 10.3% in the control group and 3% in the experimental group for this study. With a planned ratio of 2:1 between the number of cases in the experimental and control groups, and an expected 20% lost-to-follow-up rate, the initial estimated sample size for this study is 712, comprising 474 in the experimental group and 238 in the control group.Ethics and dissemination This study has been approved by Ethics committee of Beijing Friendship Hospital, Capital Medical University (approval number: 2019-P2-055-02). The results will be disseminated in major international conferences and peer-reviewed journals.Trial registration number NCT04012645.

Immune checkpoint therapy, such as programmed cell death protein 1/programmed death-ligand 1 (PD-1/PD-L1) blockade, has achieved remarkable results in treating various tumors. However, most cancer patients show a low response rate to PD-1/PD-L1 blockade, especially those with microsatellite stable/mismatch repair-proficient colorectal cancer subtypes, which indicates an urgent need for new approaches to augment the efficacy of PD-1/PD-L1 blockade. Cholesterol metabolism, which involves generating multifunctional metabolites and essential membrane components, is also instrumental in tumor development. In recent years, inhibiting proprotein convertase subtilisin/kexin type 9 (PCSK9), a serine proteinase that regulates cholesterol metabolism, has been demonstrated to be a method enhancing the antitumor effect of PD-1/PD-L1 blockade to some extent. Mechanistically, PCSK9 inhibition can maintain the recycling of major histocompatibility protein class I, promote low-density lipoprotein receptor-mediated T-cell receptor recycling and signaling, and modulate the tumor microenvironment (TME) by affecting the infiltration and exclusion of immune cells. These mechanisms increase the quantity and enhance the antineoplastic effect of cytotoxic T lymphocyte, the main functional immune cells involved in anti-PD-1/PD-L1 immunotherapy, in the TME. Therefore, combining PCSK9 inhibition therapy with anti-PD-1/PD-L1 immunotherapy may provide a novel option for improving antitumor effects and may constitute a promising research direction. This review concentrates on the relationship between PCSK9 and cholesterol metabolism, systematically discusses how PCSK9 inhibition potentiates PD-1/PD-L1 blockade for cancer treatment, and highlights the research directions in this field.

In order to solve the contradiction that the conductivity, tensile properties and antibacterial properties of traditional hydrogels cannot coexist at the same time, multifunctional CMCS/GG/PAM double-network (DN) hydrogels are prepared by introducing carboxymethyl chitosan (CMCS) into gellan gum/polyacrylamide (GG/PAM) double-network (DN) hydrogels. CMCS not only endows hydrogels with good antibacterial properties, but also enhances the interaction between GG and PAM networks. The physically crosslinked GG network and chemically crosslinked PAM network synergistically enhance the mechanical performances of the hydrogel, and the mechanical performances of hydrogel can be controlled by adjusting the dosage of GG and CMCS. The hydrogel can reach a fracture stress of 198.4 kPa and a fracture strain of 797.8%. CMCS/GG/PAM DN hydrogels have good adhesion properties, can be firmly attached to aluminum, titanium, pig skin, stainless steel and other solid surfaces. CMCS/GG/PAM DN hydrogels also have excellent biocompatibility, conductive strain sensitivity with a wide sensing range of 0–350%, short response time of 40 ms, and can monitor human movement state and facial micro-expression in real time, so as to be used as a safe antibacterial flexible sensor for human health monitoring.

Peptide-protected gold nanoclusters (AuNCs), possessing exceptional biocompatibility and remarkable physicochemical properties, have demonstrated intrinsic pharmaceutical activity in immunomodulation, making them a highly attractive frontier in the field of nanomedicine exploration. Autoimmune hepatitis (AIH) is a serious autoimmune liver disease caused by the disruption of immune balance, for which effective treatment options are still lacking. In this study, we initially identified glutathione (GSH)-protected AuNCs as a promising nanodrug candidate for AIH alleviating in a Concanavalin A (Con A)-induced mice model. However, to enhance treatment efficiency, liver-targeted delivery needs to be improved. Therefore, human serum albumin (HSA)-encapsulated AuNCs were constructed to achieve enhanced liver targeting and more potent mitigation of Con A-induced elevations in plasma aspartate transaminase (AST), alanine transaminase (ALT), and liver injury in mice. In vivo and in vitro mechanism studies indicated that AuNCs could suppress the secretion of IFN-γ by Con A-stimulated T cells and subsequently inhibit the activation of the JAK2/STAT1 pathway and eventual hepatocyte apoptosis induced by IFN-γ. These actions ultimately protect the liver from immune cell infiltration and damage caused by Con A. These findings suggest that bio-protected AuNCs hold promise as nanodrugs for AIH therapy, with their liver targeting capabilities and therapeutic efficiency being further improved via rational surface ligand engineering.

Programmed cell death protein (PD-1) is an important immunosuppressive molecule, which can inhibit interaction between PD-1 and its ligand PD-L1, further enhancing the T cell response and anti-tumor activity, which is called immune checkpoint blockade. Immunotherapy, represented by immune checkpoint inhibitors, has opened up a new era of tumor treatment and is gradually being applied to colorectal cancer recently. Immunotherapy was reported could achieve a high objective response rate (ORR) for colorectal cancer with high microsatellite instability (MSI), thus opening up a new era of colorectal cancer immunotherapy. Along with the increasing use of PD1 drugs in colorectal cancer, we should pay more attention to the adverse effects of these immune drugs while seeing the hope. Immune-related adverse events (irAEs) caused by immune activation and immune homeostasis during anti-PD-1/PD-L1 therapy can affect multi-organ and even be fatal in serious cases. Therefore, understanding irAEs is essential for their early detection and appropriate management. In this article, we review the irAEs that occur during the treatment of colorectal cancer patients with PD-1/PD-L1 drugs, analyze the current controversies and challenges, and point out future directions that should be explored, including exploring efficacy predictive markers and optimizing the paradigm of individualized immunotherapy.

PurposeSurgical complications following laparoscopic rectal cancer surgery remain a major clinical problem. The prognostic nutritional index (PNI) is reportedly associated with postoperative outcomes. We aimed to evaluate the correlation between PNI and short-term surgical complications in patients with rectal cancer after laparoscopic surgery.MethodsThe prospective clinical data of 225 patients with rectal cancer receiving laparoscopic surgery between January 2021 and April 2022 were retrospectively analyzed. The cut-off values and diagnostic accuracy of PNI preoperatively and on postoperative day (POD) 1 were determined using receiver operating characteristic (ROC) curves. Univariate and multivariate analyses were performed to identify clinical characteristics and risk factors for surgical complications.ResultsIn total, 81 (36.0%) patients developed surgical complications. The optimal cut-off value for preoperative PNI was 40.15, and that for PNI on POD 1 was 35.28. The DeLong test found no statistically between–group difference in the area under the ROC curve (P = 0.598). Multivariate analysis identified that a preoperative PNI ≤40.15 [odds ratio (OR): 2.856, 95% confidence interval (CI): 1.287–6.341, P = 0.010] and PNI on POD 1 ≤35.28 (OR: 2.773, 95% CI: 1.533–5.016, P = 0.001) were independent risk factors for surgical complications. Patients with a preoperative PNI ≤40.15 or PNI on POD 1 ≤35.28 were more likely to have surgical complications after laparoscopic surgery for rectal cancer (61.1% vs. 31.2%, P = 0.001; 53.0% vs. 28.9%, P = 0.001).ConclusionPreoperative and POD 1 PNI were independent predictors of short-term surgical complications after laparoscopic surgery for rectal cancer.

Immunotherapies, especially the programmed cell death 1/programmed cell death ligand 1 (PD-1/PD-L1) inhibitors, have revolutionized the therapeutic strategies of various cancers. As for colorectal cancer (CRC), the current clinical application of PD-1/PD-L1 inhibitors are mainly used according to the mutation pattern, which is categorized into deficient mismatch repair (dMMR)/high levels of microsatellite instability (MSI-H) and proficient mismatch repair (pMMR), or non-high levels of microsatellite instability (non-MSI-H). PD-1/PD-L1 inhibitors have been proven to have favorable outcomes against dMMR/MSI-H CRC because of more T-cell infiltration into tumor tissues. Nevertheless, the effectiveness of PD-1/PD-L1 inhibitors in pMMR/non-MSI-H CRC is still uncertain. Because of the quite-lower proportion of dMMR/MSI-H in CRC, PD-1/PD-L1 inhibitors have been reported to combine with other antitumor treatments including chemotherapy, radiotherapy, and targeted therapy for better therapeutic effect in recent clinical trials. Neoadjuvant therapy, mainly including chemotherapy and radiotherapy, not only can reduce clinical stage but also benefit from local control, which can improve clinical symptoms and the quality of life. Adding immunotherapy into neoadjuvant therapy may change the treatment strategy of primary resectable or some metastatic CRC. In this review, we focus on the development of neoadjuvant anti-PD-1/PD-L1 therapy and discuss the future perspectives in CRC.

BackgroundPrediction and management of short-term postoperative complications in patients with colorectal cancer are essential in postoperative rehabilitation. Through CT scan images, we can easily measure some parameters of abdomen anatomic characteristics. This study aimed to assess whether there is a relationship between the abdomen anatomic characteristics and short-term postoperative complications.Materials and methodsWe conducted a retrospective study. Eighty patients in each complication group and non-complication group were recruited with propensity score match. Demographics, perioperative laboratory results and surgical information were collected and compared between groups with univariate analysis. Significant elements were brought into subsequent logistic regression analysis and ROC analysis for further identification.ResultsUnivariate analysis showed that preoperative white blood cells, preoperative neutrophil counts, rectus abdominis thickness (RAT), subcutaneous fat thickness (SFT), and abdomen depth (AD) were significantly different between the complication group and non-complication group. Logistic regression analysis demonstrated that higher RAT (p = 0.002), SFT (p

Although the NSCLC diagnostic standards recommend the detection of driver gene mutation, comprehensive genomic profiling has not been used widely in clinical practice. As to the different mutation spectrum characteristics between populations, the research based on Chinese NSCLC cohort is very important for clinical practice. Therefore, we collected 563 surgical specimens from patients with non-small cell lung carcinoma and applied capture-based sequencing using eight-gene panel. We identified 556 variants, with 416 potentially actionable variants in 54.88% (309/563) patients. These single nucleotide variants, insertions and deletions were most commonly found in EGFR (55%), followed by ERBB2 (12%), KRAS (11%), PIK3CA (9%), MET (8%), BRAF (7%), DDR2 (2%), NRAS (0.3%). By using ten protein function prediction algorithms, we also identified 30 novel potentially pathogenic variants. Ninety-eight patients harbored EFGR exon 21 p.L858R mutation and the catalytic domain of the protein tyrosine kinase (PTKc) in EGFR is largely mutated. In addition, there were nine frequent pathogenic variants found in five or more patients. This data provides the potential molecular basis for directing the treatment of lung cancer.

Immune-mediated skin diseases have a high prevalence and seriously affect patients’ quality of life. Gold compounds have been considered promising therapeutic agents in dermatology, but the high incidence of adverse reactions have limited their clinical application. There is a great need to develop more effective and less toxic gold-based drugs. Gold nanoclusters fabricated by using peptides (pep-AuNCs) have appeared as potential biomedical nanomaterials because of their excellent biocompatibility, ease of fabrication and unique physicochemical properties. Glutathione (GSH) is an endogenous tripeptide and has been used for lightening the skin color. Therefore, we fabricated a well-defined gold nanocluster with GSH as an example to explore the immunomodulatory effect of AuNCs on a TNF-α-treated human keratinocyte cell line (HaCaT) in vitro, the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced irritant contact dermatitis (ICD) model and the oxazolone (OXA)-induced psoriatic model in vivo. The results indicated that topically applied AuNCs successfully attenuated the severity of ICD and psoriasis-like lesions. In vitro and in vivo, AuNCs effectively inhibited the abnormal activation of the NF-κB pathway and the consequent overexpression of proinflammatory cytokines in keratinocytes. In particular, the transactivation of IL-17A, the most important cytokine in psoriasis pathology, was effectively inhibited by AuNCs treatment. In addition, AuNCs did not show any obvious cytotoxicity in HaCaT cells at doses even up to 100 µM and did not induce any irritation in the healthy skin and major organs, which indicated their favorable biosafety. These results indicate that biocompatible pep-AuNCs might be a promising gold-based nanomedicine for the treatment of inflammatory skin diseases.

Single-layer graphene (SLG) sheets can exhibit thermoelectric properties under the control of gate voltage. The controlled factors and regulation mechanism of SLG thermoelectric properties have become research hotspots. In this paper, a SLG thermoelectric parameter model considering carrier concentration and mobility with temperature and gate voltage is proposed. Based on the proposed model, the square resistance (Rs) and Seebeck coefficient (S) of the SLG are calculated. The results show that the maximum value of Rs decreases from 5.8 KΩ to 3.2 KΩ at the Dirac voltage when the temperature increases from 100 K to 500 K. A large and stable S can be obtained at high voltages and temperatures. The maximum value of S can reach 161.3 μV/K at T = 500 K, exhibiting a more obvious thermoelectric characteristic. Simultaneously, the saturation law of the power factor (Q) with the change of gate voltage and the amplitude regulation of Q by temperature are obtained. This work can provide a theoretical basis for analyzing the thermoelectric characteristics of SLG.

Application of porous polymeric materials is severely limited by their ultralow thermal conductivities. Herein, by promoting the formation of thermal conduction pathways, we fabricated open-cellular structured polyethylene/hexagonal boron nitride hybrid thermal conductors via melt compounding plus salt leaching. The structural analyses indicate that the inclusion of hBN can enhance the open-cell level of resultant materials. X-ray diffractions confirm the high in-plane alignments of hBN in each sample. Consequently, the test results evidence the superior thermal conductivities of our samples, and the thermal conductivities of each sample are characterized as functions of hBN loadings. Ultimately, our advanced porous thermal conductor with a low hBN loading of 3.1 vol% exhibits a high specific thermal conductivity of 0.75 (W/mk)/(g/cm3), which is 82.9% higher than virgin PE and far higher than bulk PE/hBN composites. Our work also intends to reveal the architectural advantages of open-cellular, as compared with the close-one, in fabricating porous materials with highly interconnected fillers.

The foaming process and cellular morphologies of poly(styrene-co-acrylonitrile) (SAN)/chlorinated polyethylene (CPE) blends with supercritical carbon dioxide (scCO2) as a blowing agent were investigated in this study. As compared to pure SAN foam in the same batch, the foamed blends with various CPE elastomer content had smaller average pore size and larger cell density. This is probably related to the inhibition of bubble growth by elastomer, resulting in poor melt flowability and strong viscoelasticity, and the efficient bubble heterogeneous nucleation caused by numerous phase interfaces inside the incompletely compatible blend system. In addition, many tiny interconnected holes through the pore walls were formed to connect adjacent micropores in foamed blend samples. The formation mechanism of such interconnected pores is probably due to the fracture of stretched melt around the bubble from phase interfaces with weak interactions. These facts suggest an effective path to control pore size, cell density and even interconnected pores of blend foams depends on the compatibility of the blend system and difference in foamability of individual components in supercritical CO2.

BACKGROUND:Vascular pathology and dysfunction are direct life-threatening outcomes resulting from atherosclerosis or vascular injury, which are primarily attributed to contractile smooth muscle cells (SMCs) dedifferentiation and proliferation by re-entering cell cycle. Increasing evidence suggests potent protective effects of G-protein coupled estrogen receptor 1 (GPER) activation against cardiovascular diseases. However, the mechanism underlying GPER function remains poorly understood, especially if it plays a potential role in modulating coronary artery smooth muscle cells (CASMCs). METHODOLOGY/PRINCIPAL FINDINGS:The objective of our study was to understand the functional role of GPER in CASMC proliferation and differentiation in coronary arteries using from humans and swine models. We found that the GPER agonist, G-1, inhibited both human and porcine CASMC proliferation in a concentration- (10(-8) to 10(-5) M) and time-dependent manner. Flow cytometry revealed that treatment with G-1 significantly decreased the proportion of S-phase and G2/M cells in the growing cell population, suggesting that G-1 inhibits cell proliferation by slowing progression of the cell cycle. Further, G-1-induced cell cycle retardation was associated with decreased expression of cyclin B, up-regulation of cyclin D1, and concomitant induction of p21, and partially mediated by suppressed ERK1/2 and Akt pathways. In addition, G-1 induces SMC differentiation evidenced by increased α-smooth muscle actin (α-actin) and smooth muscle protein 22α (SM22α) protein expressions and inhibits CASMC migration induced by growth medium. CONCLUSION:GPER activation inhibits CASMC proliferation by suppressing cell cycle progression via inhibition of ERK1/2 and Akt phosphorylation. GPER may constitute a novel mechanism to suppress intimal migration and/or synthetic phenotype of VSMC.

Assessing construction process optimization and efficiency is crucial for the industry, yet quantitative methods for comparison are lacking. We propose the process matching degree (PMD) to quantitatively assess construction processes, enhancing efficiency and sustainability. Five primary indicators—regulations, environment, equipment, components, and organization—were identified, each of which were initially weighted equally using the weighted criteria approach (WCA). To refine the assessment, we conducted a questionnaire survey to adjust these weights based on expert feedback. Three sub-indicators were introduced for each primary indicator to increase granularity. The PMD's operability was verified through two cases study involving BIM-simulated and real construction processes. The light-steel-structure building's PMD values were 68 and 58 points, and the concrete structure's PMD was 88.25, respectively, and the reasons for these differences were analyzed. This paper introduces PMD, its evaluation indicators, and calculation method, and verifies its feasibility through cases studies and expert questionnaire surveys, providing a comprehensive PMD research methodology. [ABSTRACT FROM AUTHOR]

In the face of contemporary challenges, such as economic instability, environmental degradation, and the urgent global warming crisis, the imperative of sustainability and energy efficiency has reached unparalleled significance. Sustainability encompasses not only the natural environment, but also extends to our immediate surroundings, including the built structures and the communities they serve. Embracing this comprehensive perspective, we embarked on a mission to conceive and construct a model house that harnesses state-of-the-art energy-efficient technologies. Our goal was to seamlessly integrate these features not only to meet our sustainability objectives, but also to mitigate environmental threats.This model embodies a harmonious fusion of indigenous resources, employing locally sourced stone and employing traditional construction techniques. Through this approach, we achieved significant reductions in carbon emissions and established a framework for passive cooling and heating systems. Moreover, the design is intrinsically attuned to its contextual surroundings, preserving the diverse tapestry of regional architectural styles. This study stands as a testament to the potential of innovative design and technology in shaping a sustainable future. The research employs a multi-dimensional approach, encompassing strategies of architectural design with a traditional planning approach, sustainable material selection, energy efficiency, and life cycle assessment across a diverse set of case studies. Building energy analysis is conducted through the application of BIM (Ecotect), providing insights into how BIM can adapt and thrive in various environments. Key findings underscore that thermal performance, minimizing energy loads, and reducing carbon emissions are pivotal aspects in designating a building as both green and energy efficient. [ABSTRACT FROM AUTHOR]

The construction industry is adopting a collaborative paradigm by combining Lean construction (LC) principles and BIM capabilities. Existing studies lack sufficient case studies and performance evaluations, especially for the disassembly and reconstruction stage, creating a gap in the practical application of BIM and Lean construction (BIM-LC) in China. To bridge this gap, this study quantitatively assessed the BIM-LC methodology in a relocation project in Dezhou, China. The project employed BIM-LC practices such as the Last-Planner System (LPS), Just-In-Time (JIT), Kanban, Value-Stream Mapping (VSM), and Continuous Improvement to enhance construction efficiency and minimize waste. Two main areas of the relocation process were investigated: (i) the identification of common barriers and on-site solutions to the implementation of BIM-LC; (ii) evaluation of the construction efficiency, construction and demolition waste (CDW), and carbon-emission performance. The results showed that the BIM-LC strategy improved labor efficiency and productivity by 3.4% and 12.9%, shortened the construction period by 11 days, reduced construction costs by 8.07% (i.e., USD 9093.8), and reduced transportation costs by 12.5% (i.e., USD 1875). The CDW generation rate ranged from 1–5%, which is comparable to the rates observed in residential construction across various countries. The total weight was 1903.8 kg (10.4 kg/m2). Moreover, the carbon emission during the relocation process was 15,288.4 kgCO2e, with a carbon intensity of 83.5 kg/m2. This study extends the knowledge systems on the application of the BIM-LC method in relocation projects and supports data-driven decision making. It also plays a crucial role in fostering new markets for recycled components, contributing to the realization of a sustainable built environment. [ABSTRACT FROM AUTHOR]