50 results on '"Cong Huo"'
Search Results
2. Changes and Risk Factors of Skeletal Muscle Mass and Strength in Patients with Type 2 Diabetes over 60 Years Old: A Cross-Sectional Study from China
- Author
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Liming Hou, Yan Liu, Xing Li, Cong Huo, Xin Jia, Jie Yang, Yunzhen Lei, Rong Xu, Chao Sun, and Xiaoming Wang
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Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Objective. The accelerate loss of skeletal muscle mass, strength, and function, named sarcopenia, is a progressive and generalised skeletal muscle disorder, and it is always associated with increased outcomes including falls, frailty, and disability. Diabetes mellitus is associated with significant muscle and physical complications. We aimed at clarifying the changes and risk factors of skeletal muscle mass and strength in elderly with type 2 diabetes. Methods. The study consisted of patients with type 2 diabetes (n=120) and an older general population (n=126). The skeletal muscle mass and muscle strength, as well as the serum levels of chronic inflammation, oxidative stress, homocysteine, and insulin-like factor-1 were assessed, and the correlation and regression analysis were conducted to evaluate outcomes. Results. T2DM patients exhibited lower muscle strength compared with the non-T2DM subjects (P
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- 2020
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3. Erratum to: Inhibition of dynamin-related protein 1 protects against myocardial ischemia–reperfusion injury in diabetic mice
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Mingge Ding, Qianqian Dong, Zhenhua Liu, Zheng Liu, Yinxian Qu, Xing Li, Cong Huo, Xin Jia, Feng Fu, and Xiaoming Wang
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Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Published
- 2017
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4. On-Line Detecting Instrument of Multiple Working Modes for Optical Fiber Lines of Power System.
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Wanchang Jiang, Cong Huo, Peng Ren, Shengda Wang, and He Chen
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- 2018
5. Correlation analysis and clinical significance of Musculoskeletal Ultrasound semi-quantitative grading with bone salt metabolism, Rheumatoid factor and Erythrocyte Sedimentation rate in patients with Rheumatoid Arthritis.
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Pei-ze Song, Nan Wu, Cong Huo, and Xiao-ming Wang
- Abstract
Objective: To determine the correlation and clinical significance of musculoskeletal ultrasound semi-quantitative grading with bone salt metabolism, rheumatoid factor and erythrocyte sedimentation rate (ESR) in patients with rheumatoid arthritis. Methods: This is a clinical comparative study. A total of 240 patients with rheumatoid arthritis admitted to Baoding NO.1 Central Hospital were selected according to the DAS28 score of rheumatoid arthritis, and were divided into four groups, with 60 cases in each group from May 2020 to May 2022. The differences and correlation of musculoskeletal ultrasound semi-quantitative grading, bone metabolism indicators, erythrocyte sedimentation rate and rheumatoid factor among the four groups were statistically analyzed. Results: The scores of bone erosion, synovial hyperplasia, joint effusion and intrasynovial blood flow in Group-H were significantly higher than those in Group-R, L and M, with statistically significant differences(p=0.00). The procollagen Type-1 N-terminal propeptide(P1NP), bone-specific alkaline phosphatase(BALP) and osteoprotegerin(OPG) in Group-H were significantly lower than those in Group-R, L and M, with statistically significant differences(p=0.00); The tartrateresistant acid phosphatase(TRAC) in Group-H was significantly higher than that in Group-R, L and M, with a statistically significant difference(p=0.00). The levels of RF and ESR in Group-H were significantly higher than those in Group-R, L and M, with statistically significant differences(p=0.00). Conclusion: Musculoskeletal ultrasound semi-quantitative grading is correlated with the level of bone salt metabolism, rheumatoid factor and ESR in patients with rheumatoid arthritis. It can be combined with laboratory examination to objectively judge the severity of the course of rheumatoid arthritis. [ABSTRACT FROM AUTHOR]
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- 2023
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6. β ‐Diketiminate Aluminum Dihydride is a Valuable Precursor for the Preparation of Acyclic and Cyclic Systems
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Shuai‐Cong Huo, Yao Li, Peng‐Fei Ji, De‐Xiang Zhang, Zhi‐Jiao Zhang, Ying Yang, and Herbert W. Roesky
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Inorganic Chemistry - Published
- 2022
7. CML model based max-subset shedding for sensor streams multi-joins under limited resources.
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Wanchang Jiang and Cong Huo
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- 2010
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8. Index-Based Load Shedding for Streaming Sliding Window Joins.
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Jiadong Ren, Wanchang Jiang, and Cong Huo
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- 2007
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9. LRRC8A critically regulates myofibroblast phenotypes and fibrotic remodeling following myocardial infarction
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Xiyao Chen, Fuyang Zhang, Guangyu Hu, Xing Li, Lin Wang, Congye Li, Cong Huo, Rong Xu, Liming Hou, Ning Wang, and Xiaoming Wang
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Transforming Growth Factor beta1 ,Mice ,Phenotype ,Leucine ,Myocardial Infarction ,Medicine (miscellaneous) ,Animals ,Membrane Proteins ,Carrier Proteins ,Myofibroblasts ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Fibrosis - Published
- 2022
10. Synthesis, Characterization, and Reaction of Digermylenes
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Shuai‐Cong Huo, Yao Li, De‐Xiang Zhang, Qi Zhou, Ying Yang, and Herbert W. Roesky
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Organic Chemistry ,General Chemistry ,Biochemistry - Abstract
A series of digermylenes R(EGeL)
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- 2022
11. LRRC8A contributes to angiotensin II-induced cardiac hypertrophy by interacting with NADPH oxidases via the C-terminal leucine-rich repeat domain
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Xing Li, Rong Xu, Xin Jia, Cong Huo, Liming Hou, Yan Liu, and Xiaoming Wang
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chemistry.chemical_classification ,Gene knockdown ,Reactive oxygen species ,NADPH oxidase ,biology ,Chemistry ,Angiotensin II ,Membrane Proteins ,NADPH Oxidases ,NOX4 ,Cardiomegaly ,Leucine-rich repeat ,Biochemistry ,Cell biology ,Mice ,Cytosol ,Leucine ,Physiology (medical) ,cardiovascular system ,biology.protein ,Animals ,P22phox ,Reactive Oxygen Species ,hormones, hormone substitutes, and hormone antagonists - Abstract
Cardiac hypertrophy, an important cause of heart failure, is characterized by an increase in heart weight, the ventricular wall, and cardiomyocyte volume. The volume regulatory anion channel (VRAC) is an important regulator of cell volume. However, its role in cardiac hypertrophy remains unclear. The purpose of this study was to investigate the effect of leucine-rich repeat-containing 8A (LRRC8A), an essential component of the VRAC, on angiotensin II (AngII)-induced cardiac hypertrophy. Our results showed that LRRC8A expression, NADPH oxidase activity, and reactive oxygen species (ROS) production were increased in AngII-induced hypertrophic neonatal mouse cardiomyocytes and the myocardium of C57/BL/6 mice. In addition, AngII activated VRAC currents in cardiomyocytes. The delivery of adeno-associated viral (AAV9) bearing siRNA against mouse LRRC8A into the left ventricular wall inhibited AngII-induced cardiac hypertrophy and fibrosis. Accordingly, the knockdown of LRRC8A attenuated AngII-induced cardiomyocyte hypertrophy and VRAC currents in vitro. Furthermore, knockdown of LRRC8A suppressed AngII-induced ROS production, NADPH oxidase activity, the expression of NADPH oxidase membrane-bound subunits Nox2, Nox4, and p22phox, and the translocation of NADPH oxidase cytosolic subunits p47phox and p67phox. Immunofluorescent staining showed that LRRC8A co-localized with NADPH oxidase membrane subunits Nox2, Nox4, and p22phox. Co-immunoprecipitation and analysis of a C-terminal leucine-rich repeat domain (LRRD) mutant showed that LRRC8A physically interacts with Nox2, Nox4, and p22phox via the LRRD. Taken together, the results of this study suggested that LRRC8A might play an important role in promoting AngII-induced cardiac hypertrophy by interacting with NADPH oxidases via the LRRD.
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- 2021
12. Changes and Risk Factors of Skeletal Muscle Mass and Strength in Patients with Type 2 Diabetes over 60 Years Old: A Cross-Sectional Study from China
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Chao Sun, Liming Hou, Jie Yang, Yan Liu, Yunzhen Lei, Xiaoming Wang, Rong Xu, Xing Li, Cong Huo, and Xin Jia
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Male ,China ,Sarcopenia ,medicine.medical_specialty ,Article Subject ,Homocysteine ,Cross-sectional study ,Endocrinology, Diabetes and Metabolism ,Population ,Inflammation ,Type 2 diabetes ,medicine.disease_cause ,Risk Assessment ,Diseases of the endocrine glands. Clinical endocrinology ,chemistry.chemical_compound ,Endocrinology ,Risk Factors ,Internal medicine ,Diabetes mellitus ,medicine ,Humans ,Insulin-Like Growth Factor I ,Muscle, Skeletal ,education ,Aged ,education.field_of_study ,Hand Strength ,business.industry ,Age Factors ,Middle Aged ,RC648-665 ,medicine.disease ,Oxidative Stress ,Cross-Sectional Studies ,Diabetes Mellitus, Type 2 ,chemistry ,8-Hydroxy-2'-Deoxyguanosine ,Case-Control Studies ,Body Composition ,Female ,medicine.symptom ,business ,Biomarkers ,Oxidative stress ,Research Article - Abstract
Objective. The accelerate loss of skeletal muscle mass, strength, and function, named sarcopenia, is a progressive and generalised skeletal muscle disorder, and it is always associated with increased outcomes including falls, frailty, and disability. Diabetes mellitus is associated with significant muscle and physical complications. We aimed at clarifying the changes and risk factors of skeletal muscle mass and strength in elderly with type 2 diabetes. Methods. The study consisted of patients with type 2 diabetes ( n = 120 ) and an older general population ( n = 126 ). The skeletal muscle mass and muscle strength, as well as the serum levels of chronic inflammation, oxidative stress, homocysteine, and insulin-like factor-1 were assessed, and the correlation and regression analysis were conducted to evaluate outcomes. Results. T2DM patients exhibited lower muscle strength compared with the non-T2DM subjects ( P < 0.01 ). Among T2DM patients, serum IGF-1 levels were positively correlated with muscle strength ( r = 0.255 , P < 0.01 ) and muscle mass ( r = 0.209 , P < 0.05 ), levels of 8-OHdG were inversely correlated with muscle strength ( r = − 0.252 , P < 0.01 ), and there was a negative association between HCY and muscle mass ( r = − 0.185 , P < 0.05 ). Muscle mass and strength of patients with higher education level were significantly higher than those with lower education level ( P < 0.05 ), in male patients, muscle mass and muscle strength were significantly lower in smokers ( P < 0.01 ), and muscle mass was lower in chronic drinkers ( P < 0.05 ). Conclusions. These findings suggest that diabetic patients may be more susceptible to sarcopenia at an older age. And it also provides evidences that among elderly with diabetes mellitus, oxidative damage and HCY as well as IGF-1 are important predictors of age-dependent sarcopenia.
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- 2020
13. Norcepharadione B attenuates H2O2-induced neuronal injury by upregulating cellular antioxidants and inhibiting volume-sensitive Cl− channel
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Yan Liu, Xin Jia, Xing Li, Dongtao Li, Rong Xu, Xiaoming Wang, Cong Huo, and Liming Hou
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Chemistry ,Norcepharadione B ,Cell volume ,medicine ,Pharmacology ,medicine.disease_cause ,General Biochemistry, Genetics and Molecular Biology ,Oxidative stress - Abstract
Oxidative stress acts as an essential culprit factor in the development of stroke and Alzheimer’s disease. Norcepharadione B possesses various pharmacologic features as an extract obtained from Houttuynia cordata. Nevertheless, the anti-apoptotic and neuroprotective characteristics of norcepharadione B remain unclear. In this study, the neuronal protection effect provided by norcepharadione B against injury caused by hydrogen peroxide (H2O2) in HT22 cell as well as the fundamental mechanism was systematically explored. The neurotoxicity assays of hippocampal cells, which were co-cultured with H2O2, showed that norcepharadione B had the ability to insulate the toxicity induced by H2O2 with significant reduced cell apoptosis. Besides, norcepharadione B potentiated the activity of superoxide dismutase (SOD), increased the level of glutathione (GSH), and decreased malondialdehyde content. The H2O2-induced apoptotic protein Bax was suppressed, and the anti-apoptotic protein Bcl-2 was boosted by norcepharadione B. Norcepharadione B promoted Akt phosphorylation and further upregulated heme oxygenase (HO-1) in cells exposed to oxidative stress. However, the inductive effect of HO-1 by norcepharadione B was shut off via the PI3K/Akt inhibitor LY294002. Furthermore, 2-h incubation with H2O2 substantially increased cell volume in HT22 cells, while norcepharadione B effectively alleviated such effect by interrupting the activation of VSOR Cl− channel. Collectively, our data revealed protective properties of norcepharadione B in resisting oxidative stress induced by H2O2 through elevation of HO-1 in the dependence of PI3K/Akt and in inhibiting H2O2-induced cell swelling by VSOR Cl− channel obstruction in HT22 cells. Impact statement Norcepharadione B is an aporphine alkaloid compound extracted from Chinese herb Houttuynia cordata. It was well known for its anti-inflammatory, anti-cancer, and anti-platelet aggregation outcomes. Our study demonstrated that Norcepharadione B protected hippocampal neurons against oxidative stress and the resultant cell apoptosis upon H2O2 exposure. Meanwhile, Norcepharadione B also substantially reduced cell swelling induced by H2O2 via inhibiting VSOR Cl− channel in neurons. These findings uncovered the potential mechanisms of Norcepharadione B in protecting neuron apoptosis under oxidative stress and propose that Norcepharadione B may serve as a favorable herb medicine for restoring neuronal injury in the pathogenesis of stroke together with other neurodegenerative diseases.
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- 2019
14. Motion planning for unmanned surface vehicle based on a maneuverability mathematical model
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Dong-yu Liu, Xiao-peng Gao, and Cong Huo
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Environmental Engineering ,Ocean Engineering - Published
- 2022
15. Bisdemethoxycurcumin Protection of Cardiomyocyte Mainly Depends on Nrf2/HO-1 Activation Mediated by the PI3K/AKT Pathway
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Xiaoming Wang, Yan Liu, Cong Huo, Xing Li, Xin Jia, Rong Xu, and Yuan Xiao
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MAPK/ERK pathway ,Cardiotonic Agents ,NF-E2-Related Factor 2 ,Morpholines ,Apoptosis ,010501 environmental sciences ,Pharmacology ,Toxicology ,01 natural sciences ,Mice ,Phosphatidylinositol 3-Kinases ,03 medical and health sciences ,chemistry.chemical_compound ,Diarylheptanoids ,Bisdemethoxycurcumin ,medicine ,Animals ,Staurosporine ,Myocytes, Cardiac ,LY294002 ,Protein kinase B ,PI3K/AKT/mTOR pathway ,030304 developmental biology ,0105 earth and related environmental sciences ,Flavonoids ,chemistry.chemical_classification ,0303 health sciences ,Reactive oxygen species ,Membrane Proteins ,General Medicine ,Up-Regulation ,chemistry ,Chromones ,Curcumin ,Proto-Oncogene Proteins c-akt ,Heme Oxygenase-1 ,Signal Transduction ,medicine.drug - Abstract
Bisdemethoxycurcumin (BDMC) is one of three curcuminoids extracted from turmeric. Unlike the dominant ingredient curcumin with some intensive investigations, BDMC was recently reported to possess potent antitumor, anti-inflammatory, antiatherosclerosis, antiobesity, and antiaging effects. Considering its pharmacological effects in inflammation, atherosclerosis, and obesity, this study was designed to examine if BDMC displays cardioprotective properties. In this study, staurosporine (STS) was used to establish the cardiomyocyte injury model. Our data revealed that BDMC significantly inhibited myocardial apoptosis, improved cell survival, reduced caspase-3 activity, and diminished reactive oxygen species (ROS) production. BDMC enhanced phosphorylation of protein kinase B (AKT) and extracellular signal-regulated kinase (ERK) and up-regulated the expression of HO-1. Inhibition of HO-1 activity by using tin-protoporphyrin (SnPPIX) can restrain the antiapoptotic effect of BDMC. Furthermore, translocation of Nrf2 from the cytoplasm to the nucleus was ablated by LY294002, although only partially by PD98059. Up-regulation of HO-1 was weakly suppressed by PD98059 but strongly inhibited by LY294002. Unlike PD98059, LY294002 negated the protective effect of BDMC. These findings indicated that BDMC possessed favorable cardioprotection in a Nrf2/HO-1-dependent manner. Activation of Nrf2/HO-1 mainly depended on PI3K/AKT but not MEK/ERK signaling.
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- 2019
16. RETRACTED ARTICLE: Astragalus polysaccharide alleviates H2O2-triggered oxidative injury in human umbilical vein endothelial cells via promoting KLF2
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Yan Liu, Xing Li, Rong Xu, Cong Huo, Xin Jia, Dongtao Li, and Xiaoming Wang
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MAPK/ERK pathway ,chemistry.chemical_classification ,Reactive oxygen species ,medicine.diagnostic_test ,Chemistry ,Cell ,Biomedical Engineering ,Pharmaceutical Science ,Medicine (miscellaneous) ,02 engineering and technology ,General Medicine ,Transfection ,021001 nanoscience & nanotechnology ,Umbilical vein ,Cell biology ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,Western blot ,Apoptosis ,030220 oncology & carcinogenesis ,KLF2 ,medicine ,0210 nano-technology ,Biotechnology - Abstract
The damage of vascular endothelial cells has become an indispensable factor in the occurrence and advancement of cardiovascular diseases. In the current study, we investigated the effect of Astragalus Polysacharin (APS) on H2O2-evoked oxidative injury in HUVECs. HUVECs cells were treated by H2O2 to induce oxidative damage. Cells viability, apoptosis and reactive oxygen species (ROS) level were detected through CCK8 assay and flow cytometry. The cell growth-related proteins and heme oxygenase-1(HO-1) and KLF2 expression were evaluated via Western blot assay. The functions of KLF2 in APS and H2O2 co-disposed HUVECs were explored after si-KLF2 transfection. MEK/ERK pathway was finally measured through Western blot. We found that H2O2 stimulation-evoked HUVECs oxidative damage meanwhile impeded HO-1 expression. APS treatment effectively suppressed H2O2-induced oxidative injury in HUVECs. KLF2 and Nrf2 expression were elevated by APS and KLF2 repression abolished the protective action of APS in H2O2-triggered cell injury. MEK/ERK pathway was activated by APS treatment. Furthermore, the MEK/ERK pathway inhibitor weakened the promoting effect of APS on the expression of KLF2. In conclusion, our study reveals that APS alleviates H2O2-triggered oxidative injury in HUVECs via elevating the expression of KLF2 via the MEK/ERK pathway.
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- 2019
17. Fluorescence-Based High Throughput Screening Technologies for Natural Chloride Ion Channel Blockers
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Yan Liu, Bo Wang, Rong Xu, Yuan Xiao, Xing Li, Xiaoming Wang, Liming Hou, Cong Huo, and Xin Jia
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0301 basic medicine ,Patch-Clamp Techniques ,Recombinant Fusion Proteins ,High-throughput screening ,Green Fluorescent Proteins ,Action Potentials ,Toxicology ,Chloride ,Small Molecule Libraries ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Chloride Channels ,High-Throughput Screening Assays ,medicine ,Humans ,Ion channel ,Natural product ,Biological activity ,General Medicine ,Chloride channel blocker ,030104 developmental biology ,chemistry ,Biochemistry ,Mutagenesis, Site-Directed ,Chloride channel ,030217 neurology & neurosurgery ,HeLa Cells ,medicine.drug - Abstract
Chloride channels represent a group of potential drug targets; their blockers showed significant protecting effect on impaired cells by modulating apoptosis, autophagy, and other cell signals. However, clinical drugs with chloride channel inhibitory properties have not yet been developed. Natural product extract becomes an underlying candidate satisfied the clinical requirements for its low toxicity, low cost, and abundant sources. Here, a fluorescence-based EYFP-H148Q/I153L-HeLa cell line model was constructed by molecular cloning and verified by real-time polymerase chain reaction and Western blotting assay. By using this chloride channel blocker screening model, seven hit compounds chosen from 6988 natural compounds showed the channel blocking activity. Then the hit compounds were further validated by electrophysiological patch-clamp analysis. Our study preliminarily identified PC-4 as a new chloride channel inhibitor and demonstrated the reliability and sensitivity of fluorescence-based high throughput screening technologies for discovery of biologically active compounds from natural herbal compounds.
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- 2018
18. A Cross-sectional Survey of Prevalence and Related Factors for Frail Status Among Middle-aged and Elderly People in Chinese Communities
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Yunzhen Lei, Xing Li, Liming Hou, Xiaoming Wang, Cong Huo, Yue Hu, Rong Xu, Chao Sun, Xin Jia, and Jie Yang
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Related factors ,Cross-sectional study ,business.industry ,Environmental health ,Elderly people ,Medicine ,business - Abstract
Background: With the aggravation of social competition and work burden pressure, the health condition of the middle-aged and above population in China has declined significantly. Frailty can be used as a criterion for evaluating a person's unhealthy state. However, there is limited data on the prevalence and related factors of frail status in Chinese middle-aged and older people.The objective of this study was to explore the correlation between frail status, chronic diseases, abnormal physical examination indicators among middle-aged and older populations.Methods: Participants were 9,985 community-dwelling adults over the age of 40 years living in China. Data were from the 2015 China Health and Retirement Longitudinal Survey which was a nationally representative sample and frailty phenotype was based on Fried frailty criteria. We analyzed the demographics of participants and multivariate-adjusted related factors for frail and pre-frail population.Results: The overall prevalence of frailty and pre-frailty was 3.1% (95% CI: 2.8, 3.5) and 53.66% (95% CI: 52.7, 54.6) among the general Chinese population aged 40 years or older. Hypertension (OR: 1.8, 95% CI: 1.3, 2.593), pain (OR: 1.8, 95% CI: 1.3, 2.5), and hip fracture (OR: 2.2, 95% CI: 1.1, 4.4) were associated with prevalent frailty. Relative factors for frailty also included increased cystatin C (OR: 4.5, 95% CI: 3.0, 6.7) and glycated hemoglobin (OR: 1.2, 95% CI: 1.1, 1.4), as well as decreased peak expiratory flow (OR: 0.993, 95% CI: 0.991, 0.994).Conclusions: Fried-defined frailty and pre-frailty are highly prevalent in the Chinese population over 40 years older. Hypertension, pain, hip fracture, low education, and underweight are major related factors for frailty. The decrease in peak expiratory flow and the increase in cystatin C and glycated hemoglobin are good indicators for detecting frailty. Thus, frailty is an increasingly common condition and will become a major important health issue for people over middle age.
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- 2020
19. Norcepharadione B attenuates H
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Xin, Jia, Yan, Liu, Xing, Li, Cong, Huo, Dongtao, Li, Rong, Xu, Liming, Hou, and Xiaoming, Wang
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Neurons ,Morpholines ,Membrane Proteins ,Hydrogen Peroxide ,Isoquinolines ,Hippocampus ,Antioxidants ,Cell Line ,Up-Regulation ,Mice ,Neuroprotective Agents ,Chloride Channels ,Chromones ,Animals ,Lipid Peroxidation ,Phosphorylation ,Chloride Channel Agonists ,Proto-Oncogene Proteins c-akt ,Heme Oxygenase-1 ,Cell Size ,Original Research - Abstract
Oxidative stress acts as an essential culprit factor in the development of stroke and Alzheimer’s disease. Norcepharadione B possesses various pharmacologic features as an extract obtained from Houttuynia cordata. Nevertheless, the anti-apoptotic and neuroprotective characteristics of norcepharadione B remain unclear. In this study, the neuronal protection effect provided by norcepharadione B against injury caused by hydrogen peroxide (H(2)O(2)) in HT22 cell as well as the fundamental mechanism was systematically explored. The neurotoxicity assays of hippocampal cells, which were co-cultured with H(2)O(2), showed that norcepharadione B had the ability to insulate the toxicity induced by H(2)O(2) with significant reduced cell apoptosis. Besides, norcepharadione B potentiated the activity of superoxide dismutase (SOD), increased the level of glutathione (GSH), and decreased malondialdehyde content. The H(2)O(2)-induced apoptotic protein Bax was suppressed, and the anti-apoptotic protein Bcl-2 was boosted by norcepharadione B. Norcepharadione B promoted Akt phosphorylation and further upregulated heme oxygenase (HO-1) in cells exposed to oxidative stress. However, the inductive effect of HO-1 by norcepharadione B was shut off via the PI3K/Akt inhibitor LY294002. Furthermore, 2-h incubation with H(2)O(2) substantially increased cell volume in HT22 cells, while norcepharadione B effectively alleviated such effect by interrupting the activation of VSOR Cl(−) channel. Collectively, our data revealed protective properties of norcepharadione B in resisting oxidative stress induced by H(2)O(2) through elevation of HO-1 in the dependence of PI3K/Akt and in inhibiting H(2)O(2)-induced cell swelling by VSOR Cl(−) channel obstruction in HT22 cells. IMPACT STATEMENT: Norcepharadione B is an aporphine alkaloid compound extracted from Chinese herb Houttuynia cordata. It was well known for its anti-inflammatory, anti-cancer, and anti-platelet aggregation outcomes. Our study demonstrated that Norcepharadione B protected hippocampal neurons against oxidative stress and the resultant cell apoptosis upon H(2)O(2) exposure. Meanwhile, Norcepharadione B also substantially reduced cell swelling induced by H(2)O(2) via inhibiting VSOR Cl(−) channel in neurons. These findings uncovered the potential mechanisms of Norcepharadione B in protecting neuron apoptosis under oxidative stress and propose that Norcepharadione B may serve as a favorable herb medicine for restoring neuronal injury in the pathogenesis of stroke together with other neurodegenerative diseases.
- Published
- 2019
20. Free-running tests on a self-propelled submersible multi-state vehicle model
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Yi Zheng, Cong Huo, and Xiao-peng Gao
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Mechanism (engineering) ,Environmental Engineering ,Wing ,Computer science ,Measure (physics) ,Process (computing) ,Ocean Engineering ,Kinematics ,State (computer science) ,Underwater ,Trim ,Marine engineering - Abstract
A submersible multi-state vehicle (SMV) has been designed as an unmanned surface vehicle with global deployment capability. It can switch between several states including underwater, semi-submerged, and surface states. Free-running tests on the self-propelled model are performed to validate the multi-state transition principle of the SMV, with kinematic and hydrodynamic parameters recorded during the tests. Three-dimensional force sensors are designed to measure the wing forces, which provide insights into the mechanism of the state transition process. A series of tests conducted using program control methods verify the feasibility of the multi-state sailing and continuous state transition of the SMV. Appropriate threshold values for the wings to control depth and trim angle of the vehicle are determined through case studies on the dynamic performance. The wing forces measured show extraordinarily different characteristics and functions in different states, which provides useful information for further improvements to the design.
- Published
- 2021
21. Astragalus polysaccharide alleviates H
- Author
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Dongtao, Li, Yan, Liu, Rong, Xu, Xin, Jia, Xing, Li, Cong, Huo, and Xiaoming, Wang
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Oxidative Stress ,Gene Expression Regulation ,Cell Survival ,MAP Kinase Signaling System ,Polysaccharides ,Human Umbilical Vein Endothelial Cells ,Kruppel-Like Transcription Factors ,Humans ,Fabaceae ,Hydrogen Peroxide ,Reactive Oxygen Species - Abstract
The damage of vascular endothelial cells has become an indispensable factor in the occurrence and advancement of cardiovascular diseases. In the current study, we investigated the effect of Astragalus Polysacharin (APS) on H
- Published
- 2019
22. Preparation, Identification, and Activity Evaluation of Eight Antioxidant Peptides from Protein Hydrolysate of Hairtail (
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Xiu-Rong, Yang, Lun, Zhang, Dong-Ge, Ding, Chang-Feng, Chi, Bin, Wang, and Jian-Cong, Huo
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Protein Hydrolysates ,muscle ,Muscles ,Biphenyl Compounds ,Fishes ,antioxidant activity ,Antioxidants ,Peptide Fragments ,Article ,peptide ,Linoleic Acid ,Picrates ,hairtail (Trichiurus japonicas) ,Superoxides ,Animals ,Benzothiazoles ,Lipid Peroxidation ,Amino Acids ,Sulfonic Acids ,Peptides ,Peptide Hydrolases - Abstract
In this report, protein of hairtail (Trichiurus japonicas) muscle was separately hydrolyzed using five kinds of proteases (alcalase, trypsin, neutrase, pepsin, and papain), and the papain- and alcalase-hydrolysates showed higher 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH•) and hydroxyl radical (HO•) scavenging activity than other three protease hydrolysates. Therefore, the protein hydrolysate of hairtail muscle (HTP) was prepared using binary-enzymes hydrolysis process (papain + alcalase). Subsequently, eight antioxidant peptides were purified from HTP using membrane ultrafiltration and chromatography technology, and their amino acid sequences were identified as Gln-Asn-Asp-Glu-Arg (TJP1), Lys-Ser (TJP2), Lys-Ala (TJP3), Ala-Lys-Gly (TJP4), Thr-Lys-Ala (TJP5), Val-Lys (TJP6), Met-Lys (TJP7), and Ile-Tyr-Gly (TJP8) with molecular weights of 660.3, 233.0, 217.1, 274.1, 318.0, 245.1, 277.0, and 351.0 Da, respectively. TJP3, TJP4, and TJP8 exhibited strong scavenging activities on DPPH• (EC50 0.902, 0.626, and 0.663 mg/mL, respectively), HO• (EC50 1.740, 2.378, and 2.498 mg/mL, respectively), superoxide anion radical (EC50 2.082, 2.538, and 1.355 mg/mL, respectively), and 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical (EC50 1.652, 0.831, and 0.586 mg/mL, respectively). Moreover, TJP3, TJP4, and TJP8 showed higher reducing power and inhibiting ability on lipid peroxidation in a linoleic acid model system. These results suggested that eight isolated peptides (TJP1 to TJP8), especially TJP3, TJP4, and TJP8 might serve as potential antioxidants applied in the pharmaceutical and health food industries.
- Published
- 2018
23. Dynamic modelling of ship using gaussian processes and sg filter
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Gang Chen, Wei Wang, and Cong Huo
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History ,Computer science ,Filter (signal processing) ,Motion control ,Computer Science Applications ,Education ,System dynamics ,Noise ,symbols.namesake ,Nonlinear system ,Control theory ,Kriging ,Multicollinearity ,symbols ,Gaussian process - Abstract
Dynamic modeling of surface ships is a prerequisite for intelligent navigation and ship motion control. It is easy to ignore the nonlinear and strong coupling of ship dynamics by using traditional mechanism modeling. Moreover, the obtained parameter errors will be large due to the influence of noise and multicollinearity, it is difficult to establish a high-precision ship dynamic model. This study use a data-driven nonparametric bayesian model based on gaussian process regression on dynamic modelling of ship, it can capture the strong nonlinearity and motion coupling in ship motion, and deal with the presence of uncertainty and noise. SG filter is used to smooth the data to reduce the influence of noise on modeling. The results indicate that the gaussian processes regression and the SG filter can reproduce the ship’s motion well, which is beneficial to the further development of ship control design.
- Published
- 2021
24. Dynamin-related protein 1-mediated mitochondrial fission contributes to post-traumatic cardiac dysfunction in rats and the protective effect of melatonin
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Mingge Ding, Yuanbo Wang, Yueming Wang, Xiaoming Wang, Xing Li, Zeyang Li, Jianming Pei, Jiao Ning, Zhenhua Liu, Na Feng, Xin Jia, Cong Huo, Feng Fu, and Rong Xu
- Subjects
0301 basic medicine ,Cardiac function curve ,Dynamins ,Male ,medicine.medical_specialty ,Apoptosis ,Mitochondrion ,Mitochondrial Dynamics ,Melatonin ,Rats, Sprague-Dawley ,03 medical and health sciences ,DNM1L ,0302 clinical medicine ,Endocrinology ,Internal medicine ,medicine ,Animals ,biology ,Chemistry ,Cytochrome c ,Mitochondria ,Rats ,030104 developmental biology ,biology.protein ,Phosphorylation ,Mitochondrial fission ,Tumor necrosis factor alpha ,030217 neurology & neurosurgery ,medicine.drug ,Signal Transduction - Abstract
Mechanical trauma (MT) causes myocardial injury and cardiac dysfunction. However, the underlying mechanism remains largely unclear. This study investigated the role of mitochondrial dynamics in post-traumatic cardiac dysfunction and the protective effects of melatonin. Adult male Sprague Dawley rats were subjected to 5-minute rotations (200 revolutions at a rate of 40 rpm) to induce MT model. Melatonin was administrated intraperitoneally 5 minute after MT. Mitochondrial morphology, myocardial injury, and cardiac function were determined in vivo. There was smaller size of mitochondria and increased number of mitochondria per μm2 in the hearts after MT when the secondary myocardial injury was induced. Melatonin treatment at the dose of 30 mg/kg reduced serine 616 phosphorylation of Drp1 and inhibited mitochondrial Drp1 translocation and mitochondrial fission in the hearts of rats subjected to MT, which contributed to the reduction of myocardial injury and the improvement of cardiac function. In vitro, H9c2 cells cultured in 20% traumatic plasma (TP) for 12 hour showed enhanced mitochondrial fission, mitochondrial membrane potential (∆Ψm) loss, mitochondrial cytochrome c release, and decreased mitochondrial complex I-IV activities. Pretreatment with melatonin (100 μmol/L) efficiently inhibited TP-induced mitochondrial fission, ∆Ψm loss, cytochrome c release, and improved mitochondrial function. Melatonin's protective effects were attributed to its role in suppressing plasma TNF-α overproduction, which was responsible for Drp1-mediated mitochondrial fission. Taken together, our results demonstrate for the first time that abnormal mitochondrial dynamics is involved in post-traumatic cardiac dysfunction. Melatonin has significant pharmacological potential in protecting against MT-induced cardiac dysfunction by preventing excessive mitochondrial fission.
- Published
- 2017
25. Punicalagin Pretreatment Attenuates Myocardial Ischemia-Reperfusion Injury via Activation of AMPK
- Author
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Feng Fu, Xing Li, Mingge Ding, Zhenghua Liu, Xin Jia, Jie Wang, Wei Chen, Yin Wang, Di Sun, Xiaoming Wang, and Cong Huo
- Subjects
0301 basic medicine ,Male ,Cardiotonic Agents ,Premedication ,Apoptosis ,Myocardial Reperfusion Injury ,Pharmacology ,AMP-Activated Protein Kinases ,medicine.disease_cause ,Neuroprotection ,Antioxidants ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,Lactate dehydrogenase ,Medicine ,Animals ,Creatine Kinase, MB Form ,Myocytes, Cardiac ,Ligation ,Punicalagin ,Cardioprotection ,L-Lactate Dehydrogenase ,business.industry ,Myocardium ,AMPK ,Heart ,General Medicine ,medicine.disease ,Adenosine ,Coronary Vessels ,Hydrolyzable Tannins ,Rats ,Disease Models, Animal ,Oxidative Stress ,030104 developmental biology ,Complementary and alternative medicine ,chemistry ,Biochemistry ,business ,Reperfusion injury ,Oxidative stress ,medicine.drug - Abstract
Punicalagin (PUN), a major bioactive component in pomegranate juice, has been proven to exert neuroprotective effects against cerebral ischemia/reperfusion (I/R) insult via anti-oxidant properties. This study aims to investigate whether PUN provides cardioprotection against myocardial I/R (MI/R) injury and the underlying mechanisms. PUN (30[Formula: see text]mg/kg/d) or vehicle was intragastrically administered to Sprague-Dawley rats for one week before the operation. MI/R was induced by ligating the left anterior descending coronary artery for 30[Formula: see text]min and subsequent reperfusion for 3[Formula: see text]h. PUN pretreatment conferred cardioprotective effects against MI/R injury by improving cardiac function, limiting infarct size, reducing serum creatine kinase-MB and lactate dehydrogenase activities, and suppressing cardiomyocyte apoptosis. Moreover, PUN pretreatment inhibited I/R-induced myocardial oxidative stress as evidenced by decreased generation of superoxide content and malonaldialdehyde formation and increased antioxidant capability. Furthermore, PUN pretreatment increased adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) phosphorylation in I/R hearts. AMPK inhibitor compound c inhibited PUN-enhanced AMPK phosphorylation, and blunted PUN-mediated anti-oxidative effects and cardioprotection. These results indicate for the first time that PUN pretreatment protect against I/R-induced oxidative stress and myocardial injury via activation of AMPK.
- Published
- 2017
26. Preparation, Identification, and Activity Evaluation of Eight Antioxidant Peptides from Protein Hydrolysate of Hairtail (Trichiurus japonicas) Muscle
- Author
-
Bin Wang, Chang-Feng Chi, Jian-Cong Huo, Xiu-Rong Yang, Dong-Ge Ding, and Lun Zhang
- Subjects
endocrine system ,animal structures ,Antioxidant ,hairtail (Trichiurus japonicas) ,muscle ,030309 nutrition & dietetics ,DPPH ,medicine.medical_treatment ,Linoleic acid ,antioxidant activity ,Pharmaceutical Science ,Hydrolysate ,Lipid peroxidation ,03 medical and health sciences ,chemistry.chemical_compound ,fluids and secretions ,0404 agricultural biotechnology ,Drug Discovery ,medicine ,Food science ,lcsh:QH301-705.5 ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,0303 health sciences ,Protease ,ABTS ,04 agricultural and veterinary sciences ,040401 food science ,peptide ,Papain ,lcsh:Biology (General) ,chemistry - Abstract
In this report, protein of hairtail (Trichiurus japonicas) muscle was separately hydrolyzed using five kinds of proteases (alcalase, trypsin, neutrase, pepsin, and papain), and the papain- and alcalase-hydrolysates showed higher 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH&bull, ) and hydroxyl radical (HO&bull, ) scavenging activity than other three protease hydrolysates. Therefore, the protein hydrolysate of hairtail muscle (HTP) was prepared using binary-enzymes hydrolysis process (papain + alcalase). Subsequently, eight antioxidant peptides were purified from HTP using membrane ultrafiltration and chromatography technology, and their amino acid sequences were identified as Gln-Asn-Asp-Glu-Arg (TJP1), Lys-Ser (TJP2), Lys-Ala (TJP3), Ala-Lys-Gly (TJP4), Thr-Lys-Ala (TJP5), Val-Lys (TJP6), Met-Lys (TJP7), and Ile-Tyr-Gly (TJP8) with molecular weights of 660.3, 233.0, 217.1, 274.1, 318.0, 245.1, 277.0, and 351.0 Da, respectively. TJP3, TJP4, and TJP8 exhibited strong scavenging activities on DPPH&bull, (EC50 0.902, 0.626, and 0.663 mg/mL, respectively), HO&bull, (EC50 1.740, 2.378, and 2.498 mg/mL, respectively), superoxide anion radical (EC50 2.082, 2.538, and 1.355 mg/mL, respectively), and 2,2&prime, azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical (EC50 1.652, 0.831, and 0.586 mg/mL, respectively). Moreover, TJP3, TJP4, and TJP8 showed higher reducing power and inhibiting ability on lipid peroxidation in a linoleic acid model system. These results suggested that eight isolated peptides (TJP1 to TJP8), especially TJP3, TJP4, and TJP8 might serve as potential antioxidants applied in the pharmaceutical and health food industries.
- Published
- 2019
27. Norcepharadione B attenuates H2O2-induced neuronal injury by upregulating cellular antioxidants and inhibiting volume-sensitive Cl- channel.
- Author
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Xin Jia, Yan Liu, Xing Li, Cong Huo, Dongtao Li, Rong Xu, Liming Hou, and Xiaoming Wang
- Published
- 2019
- Full Text
- View/download PDF
28. Activation of volume-sensitive Cl- channel mediates autophagy-related cell death in myocardial ischaemia/reperfusion injury
- Author
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Hong Li, Xin Jia, Yuesheng Xia, Mingming Zhang, Rong Xu, Yan Liu, Ning Wang, Xing Li, Tong Xia, Lin Wang, Cong Huo, and Xiaoming Wang
- Subjects
0301 basic medicine ,Male ,Myocardial Ischemia ,Apoptosis ,Pharmacology ,medicine.disease_cause ,ischemia/reperfusion injury ,Rats, Sprague-Dawley ,0302 clinical medicine ,Medicine ,Hypoxia ,chemistry.chemical_classification ,reactive oxygen species ,Hydrogen-Ion Concentration ,cell death ,Oncology ,030220 oncology & carcinogenesis ,Reperfusion Injury ,Cytokines ,VSOR Cl− channel ,RNA Interference ,Microtubule-Associated Proteins ,Programmed cell death ,autophagy ,Ischemia ,03 medical and health sciences ,Chloride Channels ,Lysosomal-Associated Membrane Protein 2 ,Pathology Section ,Animals ,Channel blocker ,Cell Proliferation ,Inflammation ,Reactive oxygen species ,business.industry ,Adenine ,Myocardium ,Autophagy ,medicine.disease ,Research Paper: Pathology ,Rats ,Oxidative Stress ,030104 developmental biology ,chemistry ,Immunology ,business ,Lysosomes ,Reperfusion injury ,Oxidative stress - Abstract
// Yuesheng Xia 1,* , Yan Liu 1,* , Tong Xia 2,* , Xing Li 1 , Cong Huo 1 , Xin Jia 1 , Lin Wang 1 , Rong Xu 1 , Ning Wang 1 , Mingming Zhang 3 , Hong Li 1 and Xiaoming Wang 1 1 Department of Geriatrics, Xijing Hospital, Fourth Military Medical University, Xi’an, China 2 Department of Preventive Medicine, Sun Yat-sen University, Guangzhou, China 3 Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an, China * These authors have contributed equally to this work Correspondence to: Xiaoming Wang, email: // Keywords : ischemia/reperfusion injury, VSOR Cl - channel, reactive oxygen species, autophagy, cell death, Pathology Section Received : April 08, 2016 Accepted : June 03, 2016 Published : June 14, 2016 Abstract Excessive reactive oxygen species (ROS) plays an important role in myocardial ischemia/reperfusion (I/R) injury, which triggers not only myocardial cellular apoptosis but also autophagy-related cell death, in which volume-sensitive outwardly rectifying (VSOR) Cl - channel-activated by ROS contributes to cell apoptotic volume decrease, playing an incipient incident of cellular apoptosis. However, whether VSOR Cl - channel concurrently participates in autophagy-related cell death regulation remains unclear. To illuminate the issue, studies underwent in myocardial vitro and vivo I/R model. Rats were performed to ischemia 30 minutes and subsequent reperfusion 24-96 hours, ROS scavenger (NAC), VSOR Cl - channel blocker (DCPIB) and autophagy inhibitor (3MA) were administered respectively. Results showed that oxidative stress, LC3-II stain and inflammation in myocardial tissue were markedly increased, lysosome associated membrane protein-2 (LAMP2) were significantly reduced with I/R group as compared with sham group, reperfusion significantly led to damage in myocardial tissue and heart function, whereas the disorder could be rescued through these agents. Moreover, primary neonatal rat cardiomyocytes hypoxia/reoxygenation model were administered, results showed that VSOR Cl - channel-activated by reoxygenation could cause both cell volume decrease and intracellular acidification, which further increased LC3 and depleted of LAMP2, resulting in autophagy-related cell death. Interestingly, VSOR Cl - channel-blocked by DCPIB could stably maintain the cell volume, intracellular pH, abundant LAMP2 and autophagic intensity regardless of ROS intension derived from reoxygenation injury or adding H 2 O 2 . These results first demonstrate that VSOR Cl - channel-activated is a pivotal event to trigger autophagy-related death, which reveals a novel therapeutic target to decrease myocardial I/R injury.
- Published
- 2016
29. Test research on PM2.5 emissions of the vehicle
- Author
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Hong-Mei Wang, Zhan-Yu Wang, Cong-Cong Huo, and Xin-Guang Li
- Subjects
Environmental science ,Automotive engineering ,Test (assessment) - Published
- 2015
30. A novel endoplasmic reticulum stress‑induced apoptosis model using tunicamycin in primary cultured neonatal rat cardiomyocytes
- Author
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Mingzhi Shen, Xing Li, Xiaoming Wang, Lin Wang, Xiaowang Guo, Qiao Xue, Cong Huo, and Li Fan
- Subjects
Cancer Research ,Cell Survival ,Primary Cell Culture ,Apoptosis ,CHOP ,Biology ,Biochemistry ,Flow cytometry ,chemistry.chemical_compound ,Genetics ,medicine ,Animals ,MTT assay ,Myocytes, Cardiac ,Viability assay ,Molecular Biology ,Cell damage ,Cells, Cultured ,Heat-Shock Proteins ,medicine.diagnostic_test ,Endoplasmic reticulum ,Tunicamycin ,medicine.disease ,Endoplasmic Reticulum Stress ,Molecular biology ,Rats ,Oncology ,chemistry ,Animals, Newborn ,Gene Expression Regulation ,Molecular Medicine ,Transcription Factor CHOP - Abstract
Endoplasmic reticulum (ER) stress is key in the development of cardiovascular diseases. However, there is a lack of a systemic ER stress‑induced cardiomyocyte apoptosis model. In the present study, primary cultured neonatal rat cardiomyocytes were exposed to tunicamycin. Cell viability was determined by an MTT assay, and cell damage was detected by a lactose dehydrogenase assay. Flow cytometry was used and the activity of caspase‑3 was analyzed in order to measure apoptosis. Reverse transcription-quantitative polymerase chain reaction and western blotting were used to examine the expression of glucose‑regulated protein 78‑kDa (GRP78) and C/EBP homologous protein (CHOP). As a result, tunicamycin significantly increased cardiomyocyte injury, which occurred in a time- and concentration‑dependent manner. In addition, tunicamycin treatment resulted in apoptosis of cardiomyocytes. Molecularly, tunicamycin (100 ng/ml) increased the levels of GRP78 and CHOP 6 h after administration. In addition, GRP78 and CHOP reached maximum mRNA and protein levels 24 h after administration. In conclusion, the results implicate that the tunicamycin‑induced ER stress‑induced apoptotic model was successfully constructed in cultured neonatal rat cardiomyocytes. A 100 ng/ml concentration of tunicamycin was selected, and MTT, LDH release and flow cytometry assay was at 72 h. In addition, GRP78 and GRP94 were detected 24 h following administration. The results of the present study indicate a novel experimental basis for the investigation of ERS-induced cardiac apoptosis.
- Published
- 2014
31. [Case of bilateral diaphragm paralysis]
- Author
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Kai-xue, Jia, Ying-hui, Wang, and Cong-huo, Tian
- Subjects
Male ,Diaphragm ,Acupuncture Therapy ,Humans ,Middle Aged ,Respiratory Paralysis - Published
- 2011
32. CML model based max-subset shedding for sensor streams multi-joins under limited resources
- Author
-
Cong Huo and Wanchang Jiang
- Subjects
Memory management ,Distributed database ,Data stream mining ,Computer science ,Distributed computing ,Sliding window protocol ,Real-time computing ,Joins ,Approximation algorithm ,Algorithm design ,Context (language use) ,Central processing unit - Abstract
Join queries over wireless sensor data streams need to be processed immediately to keep up with the input streams. Many existing algorithms do not solve the problem in context of both limited CPU and memory resources. In this paper, we propose two CML statistic model based approximate sliding window multi-joins algorithms for the system that both CPU and memory is limited, and a maximum subset of the exact multi-join result is obtained. To shed the load effectively and produce as many join results as possible with the limited amount of resources, both the statistical information of join attributes value of each stream and the relationship between CPU and memory resource is completely considered. The CML statistic model is designed for obtaining and maintaining the statistical information dynamically. With the model, semantic load shedding algorithms are proposed over streaming sliding window multi-join under both limited CPU and memory resource. And a maximizing multi-join output can be generated without delay. Experimental results show that our approach is more efficient than other approach when both the CPU and memory resource are insufficient to keep pace with input streams.
- Published
- 2010
33. Mining Closed Frequent Itemsets in Sliding Window over Data Streams
- Author
-
Jiadong Ren and Cong Huo
- Subjects
Data stream mining ,Computer science ,Group method of data handling ,Hash function ,InformationSystems_DATABASEMANAGEMENT ,Data structure ,computer.software_genre ,Set (abstract data type) ,Tree (data structure) ,ComputingMethodologies_PATTERNRECOGNITION ,Sliding window protocol ,Algorithm design ,Data mining ,computer - Abstract
As one of the most important problems in data streams mining, many studies have been done on mining closed frequent itemsets. However mining closed frequent itemsets in data streams has not been well addressed. In this paper, we design HCI-Mtree (Hash-based Closed Itemsets Monolayer tree) to maintain the complete set of current closed itemsets. In HCI-Mtree, the itemsets with the same frequency are linked to the same hash-based counter. To mining closed frequent itemsets in sliding window over data streams, we propose a novel approach HCFI (algorithm based on HCI-Mtree for mining Closed Frequent Itemsets). Vertical representation of transactions is utilized in our algorithm to save processing time and space consuming. Our experiments show that HCFI has good performance especially when the window size is large.
- Published
- 2008
34. Authentication and Key Establishment Scheme Based on Token for Mobile Commerce
- Author
-
Cong Huo, Wanchang Jiang, and Jiadong Ren
- Subjects
Binary tree ,Computer science ,Aggregate (data warehouse) ,Sampling (statistics) ,Window (computing) ,Joins ,computer.software_genre ,Random binary tree ,Histogram ,Join (sigma algebra) ,Data mining ,Algorithm ,computer ,Computer Science::Databases - Abstract
Two novel sampling approaches are proposed to obtain a random sample of exact streaming window join result. Without assuming any model of stream arrivals, the frequency of join attribute values for various basic periods can be obtained by a frequency balanced binary tree histogram (FATH) which is constructed for each stream. The frequency for the future window can be computed by linear regression with the help of the information in the FATH. With the random sample of exact join result produced, a windowed aggregate over the exact join results can be unbiasedly and accurately estimated. Experimental results show that our approach is more efficient than other approach for arbitrary streams.
- Published
- 2007
35. An Adaptive and Distributed Clustering Scheme for Wireless Sensor Networks
- Author
-
Yadong Zhang, Xiaojian Zhang, Cong Huo, and Wanchang Jiang
- Subjects
Hash join ,Computer science ,Sliding window protocol ,Histogram ,Hash function ,Real-time computing ,Join (sigma algebra) ,Joins ,Nested loop join ,Cluster analysis ,Algorithm - Abstract
Join operator has become more and more important in the context of data stream. Most join algorithms over streams to date are based on nested loop joins or hash joins. However, these time expensive algorithms can not suit for CPU-limited case. In this paper, a clustering-based variable-width histogram is designed for obtaining value distribution of tuples in the sliding windows, and some important characteristics of tuples can be retained. Semi-hash tables can be constructed by using the histogram. Our sliding window semi-hash multi-join algorithm can minimize the processing time cost of join and produce an accurate join result much earlier. Experimental results show that our approach is more efficient than other approaches.
- Published
- 2007
36. Random Sampling over Streaming Window Joins
- Author
-
Jiadong Ren, Wanchang Jiang, and Cong Huo
- Subjects
Cloud computing security ,Computer science ,business.industry ,Covert channel ,Data security ,Trusted Computing ,Computer security model ,Computer security ,computer.software_genre ,Trusted computing base ,Direct Anonymous Attestation ,Information flow (information theory) ,business ,computer ,Computer Science::Cryptography and Security ,Computer network - Abstract
In this paper, we introduce a new information flow model for trusted computing systems.Different from traditional system protection models, such as access control matrix model, this model analyze the information flow in the system by tracing the operation sequences , and explains that the security status of a trusted computing system is the security attributes of information flows from input port to output port.With this model, we discuss the information channels in a trusted computing systems, and propose a "normal information flow theorem" which gives a necessary condition of the existence of normal information flow.
- Published
- 2007
37. Index-Based Load Shedding for Streaming Sliding Window Joins
- Author
-
Cong Huo, Wanchang Jiang, and Jiadong Ren
- Subjects
Data stream ,Index (economics) ,Computer science ,Sliding window protocol ,Search engine indexing ,Locality ,Real-time computing ,Joins ,Tuple ,Cluster analysis ,Algorithm - Abstract
We present a novel clustering-based indexing load shedding technique for sliding window joins (CILS). When the details of the distribution of streams are unknown, to obtain the statistics of data, we dynamically maintain a dual indices model (CRA-index and CCRA-index) for each sliding window. The indices are constructed and maintained by clustering technique, and the locality of data is retained. When CPU capacity is insufficient, our index-based load shedding technique is used by utilizing the CRA-index and CCRA-index, a part of tuples are selected and are kept from performing probing, and finally maximum subset join outputs are produced. The indices are space (a fixed amount of memory is used) and time efficient. Experimental results on synthetic and real life data show that our index-based load shedding approach obtains max-subset results effectively, and outperforms the existing load shedding strategies under the same conditions.
- Published
- 2007
38. Efficient Load Shedding for Streaming Sliding Window Joins
- Author
-
Wanchang Jiang, Cong Huo, and Jiadong Ren
- Subjects
Data stream mining ,Computer science ,Histogram ,Sliding window protocol ,Real-time computing ,Range (statistics) ,Joins ,Table (database) ,Cluster analysis - Abstract
We present a novel load shedding technique, called range loading shedding (denoted as RANGE), for sliding window joins when CPU capacity is insufficient in the system and the details of the distribution of streams are unknown. To obtain the statistics of data, we dynamically maintain clustering range histogram (CR-histogram) and average density counter table (ADC-table) for each sliding window. The CR-Histogram is constructed and maintained by clustering technique with a fixed amount of memory. When CPU capacity is insufficient, the RANGE technique is used to select tuples to be processed by utilizing the CR-Histogram and ADC-table, and then produces maximum subset join outputs. Experimental results on synthetic and real life data show that Range load shedding approach obtains max-subset results effectively, and outperforms the existing load shedding strategies.
- Published
- 2007
39. Inhibition of dynamin-related protein 1 protects against myocardial ischemia-reperfusion injury in diabetic mice.
- Author
-
Mingge Ding, Qianqian Dong, Zhenghua Liu, Zheng Liu, Yinxian Qu, Xing Li, Cong Huo, Xin Jia, Feng Fu, and Xiaoming Wang
- Subjects
CORONARY disease ,LABORATORY mice ,DYNAMIN (Genetics) ,STREPTOZOTOCIN ,REPERFUSION - Abstract
Background: Many cardioprotective pharmacological agents failed to exert their protective effects in diabetic hearts subjected to myocardial ischemia/reperfusion (MI/R). Identify the molecular basis linking diabetes with MI/R injury is scientifically important and may provide effective therapeutic approaches. Dynamin-related protein 1 (Drp1)-mediated mitochondrial fission plays an important role in MI/R injury under non-diabetic conditions. Importantly, recent studies indicated that Drp1-mediated mitochondrial fission is enhanced in the myocardium of diabetic mice. The above evidences suggested that Drp1 may be one critical molecule linking diabetes with MI/R injury. We hypothesized that inhibition of Drp1 may be effective to reduce MI/R injury in diabetic hearts. Methods: High-fat diet and streptozotocin-induced diabetic mice were subjected to MI/R or sham operation. Mdivi-1 (1.2 mg/kg), a small molecule inhibitor of Drp1 or vehicle was administrated 15 min before the onset of reperfusion. Outcome measures included mitochondrial morphology, mitochondrial function, myocardial injury, cardiac function and oxidative stress. Results: Mitochondrial fission was significantly increased following MI/R as evidenced by enhanced translocation of Drp1 to mitochondria and decreased mitochondrial size. Delivery of Mdivi-1 into diabetic mice markedly inhibited Drp1 translocation to the mitochondria and reduced mitochondrial fission following MI/R. Inhibition of Drp1 in diabetic hearts improved mitochondrial function and cardiac function following MI/R. Moreover, inhibition of Drp1 reduced myocardial infarct size and serum cardiac troponin I and lactate dehydrogenase activities. These cardioprotective effects were associated with decreased cardiomyocyte apoptosis and malondialdehyde production and increased activities of antioxidant enzyme manganese superoxide dismutase. Conclusions: Pharmacological inhibition of Drp1 prevents mitochondrial fission and reduces MI/R injury in diabetic mice. The findings suggest Drp1 may be a potential novel therapeutic target for diabetic cardiac complications. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
40. Test research on PM2.5 emissions of the vehicle.
- Author
-
Xin-Guang Li, Zhan-Yu Wang, Hong-Mei Wang, and Cong-Cong Huo
- Subjects
PARTICULATE matter ,MOTOR vehicle fuel systems ,WASTE gases ,VOLUMETRIC analysis ,TWO-phase flow - Published
- 2015
41. A novel endoplasmic reticulum stress-induced apoptosis model using tunicamycin in primary cultured neonatal rat cardiomyocytes.
- Author
-
MINGZHI SHEN, LIN WANG, XIAOWANG GUO, QIAO XUE, CONG HUO, XING LI, LI FAN, and XIAOMING WANG
- Subjects
ENDOPLASMIC reticulum ,DISEASES ,PSYCHOLOGICAL stress ,APOPTOSIS ,LABORATORY rats ,HEART cells ,TUNICAMYCIN - Abstract
Endoplasmic reticulum (ER) stress is key in the development of cardiovascular diseases. However, there is a lack of a systemic ER stress-induced cardiomyocyte apoptosis model. In the present study, primary cultured neonatal rat cardiomyocytes were exposed to tunicamycin. Cell viability was determined by an MTT assay, and cell damage was detected by a lactose dehydrogenase assay. Flow cytometry was used and the activity of caspase-3 was analyzed in order to measure apoptosis. Reverse transcription-quantitative polymerase chain reaction and western blotting were used to examine the expression of glucose-regulated protein 78-kDa (GRP78) and C/EBP homologous protein (CHOP). As a result, tunicamycin significantly increased cardiomyocyte injury, which occurred in a time- and concentration-dependent manner. In addition, tunicamycin treatment resulted in apoptosis of cardiomyocytes. Molecularly, tunicamycin (100 ng/ml) increased the levels of GRP78 and CHOP 6 h after administration. In addition, GRP78 and CHOP reached maximum mRNA and protein levels 24 h after administration. In conclusion, the results implicate that the tunicamycin-induced ER stress-induced apoptotic model was successfully constructed in cultured neonatal rat cardiomyocytes. A 100 ng/ml concentration of tunicamycin was selected, and MTT, LDH release and flow cytometry assay was at 72 h. In addition, GRP78 and GRP94 were detected 24 h following administration. The results of the present study indicate a novel experimental basis for the investigation of ERS-induced cardiac apoptosis. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
42. Research on Static Characteristics of Six-Dimension Force Sensor
- Author
-
Wen-bin, Xiao, primary, Cong, Huo, additional, and Wen-cai, Dong, additional
- Published
- 2012
- Full Text
- View/download PDF
43. Efficient Load Shedding for Streaming Sliding Window Joins.
- Author
-
Jia-Dong Ren, Wan-Chang Jiang, and Cong Huo
- Published
- 2007
- Full Text
- View/download PDF
44. Clustering-Variable-Width Histogram Based Window Semi-hash Multi-join over Streams.
- Author
-
Xiaojian Zhang, Wanchang Jiang, Yadong Zhang, and Cong Huo
- Published
- 2007
- Full Text
- View/download PDF
45. Two Modes Power Optical Fiber Network Monitoring with Protective Optical Path and Fault Self-Recovery.
- Author
-
Wanchang Jiang, Cong Huo, Meng Xu, Peng Ren, Shengda Wang, and He Chen
- Published
- 2018
- Full Text
- View/download PDF
46. Inhibition of dynamin-related protein 1 protects against myocardial ischemia–reperfusion injury in diabetic mice
- Author
-
Yinxian Qu, Zheng Liu, Xing Li, Xin Jia, Cong Huo, Mingge Ding, Zhenghua Liu, Qianqian Dong, Feng Fu, and Xiaoming Wang
- Subjects
0301 basic medicine ,Cardiac function curve ,Dynamins ,Male ,medicine.medical_specialty ,endocrine system ,Endocrinology, Diabetes and Metabolism ,Myocardial Infarction ,Apoptosis ,Myocardial Reperfusion Injury ,030204 cardiovascular system & hematology ,Mitochondrion ,Pharmacology ,Mitochondrial Size ,medicine.disease_cause ,Diet, High-Fat ,Mitochondrial Dynamics ,Mitochondria, Heart ,Streptozocin ,Diabetes Mellitus, Experimental ,03 medical and health sciences ,DNM1L ,0302 clinical medicine ,Internal medicine ,Malondialdehyde ,medicine ,Animals ,Myocytes, Cardiac ,Quinazolinones ,L-Lactate Dehydrogenase ,business.industry ,Superoxide Dismutase ,Troponin I ,Cardiovascular Agents ,medicine.disease ,Mice, Inbred C57BL ,Oxidative Stress ,030104 developmental biology ,Cardiovascular agent ,Cardiology ,Mitochondrial fission ,Erratum ,business ,Cardiology and Cardiovascular Medicine ,Reperfusion injury ,Oxidative stress ,Signal Transduction - Abstract
Many cardioprotective pharmacological agents failed to exert their protective effects in diabetic hearts subjected to myocardial ischemia/reperfusion (MI/R). Identify the molecular basis linking diabetes with MI/R injury is scientifically important and may provide effective therapeutic approaches. Dynamin-related protein 1 (Drp1)-mediated mitochondrial fission plays an important role in MI/R injury under non-diabetic conditions. Importantly, recent studies indicated that Drp1-mediated mitochondrial fission is enhanced in the myocardium of diabetic mice. The above evidences suggested that Drp1 may be one critical molecule linking diabetes with MI/R injury. We hypothesized that inhibition of Drp1 may be effective to reduce MI/R injury in diabetic hearts. High-fat diet and streptozotocin-induced diabetic mice were subjected to MI/R or sham operation. Mdivi-1 (1.2 mg/kg), a small molecule inhibitor of Drp1 or vehicle was administrated 15 min before the onset of reperfusion. Outcome measures included mitochondrial morphology, mitochondrial function, myocardial injury, cardiac function and oxidative stress. Mitochondrial fission was significantly increased following MI/R as evidenced by enhanced translocation of Drp1 to mitochondria and decreased mitochondrial size. Delivery of Mdivi-1 into diabetic mice markedly inhibited Drp1 translocation to the mitochondria and reduced mitochondrial fission following MI/R. Inhibition of Drp1 in diabetic hearts improved mitochondrial function and cardiac function following MI/R. Moreover, inhibition of Drp1 reduced myocardial infarct size and serum cardiac troponin I and lactate dehydrogenase activities. These cardioprotective effects were associated with decreased cardiomyocyte apoptosis and malondialdehyde production and increased activities of antioxidant enzyme manganese superoxide dismutase. Pharmacological inhibition of Drp1 prevents mitochondrial fission and reduces MI/R injury in diabetic mice. The findings suggest Drp1 may be a potential novel therapeutic target for diabetic cardiac complications.
- Full Text
- View/download PDF
47. Prospects for intelligent rehabilitation techniques to treat motor dysfunction
- Author
-
Cong-Cong Huo, Ya Zheng, Wei-Wei Lu, Teng-Yu Zhang, Dai-Fa Wang, Dong-Sheng Xu, and Zeng-Yong Li
- Subjects
brain ,central nerve ,injury ,nerve ,nerve function ,neurogenesis ,plasticity ,repair ,spinal cord ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
More than half of stroke patients live with different levels of motor dysfunction after receiving routine rehabilitation treatments. Therefore, new rehabilitation technologies are urgently needed as auxiliary treatments for motor rehabilitation. Based on routine rehabilitation treatments, a new intelligent rehabilitation platform has been developed for accurate evaluation of function and rehabilitation training. The emerging intelligent rehabilitation techniques can promote the development of motor function rehabilitation in terms of informatization, standardization, and intelligence. Traditional assessment methods are mostly subjective, depending on the experience and expertise of clinicians, and lack standardization and precision. It is therefore difficult to track functional changes during the rehabilitation process. Emerging intelligent rehabilitation techniques provide objective and accurate functional assessment for stroke patients that can promote improvement of clinical guidance for treatment. Artificial intelligence and neural networks play a critical role in intelligent rehabilitation. Multiple novel techniques, such as brain-computer interfaces, virtual reality, neural circuit-magnetic stimulation, and robot-assisted therapy, have been widely used in the clinic. This review summarizes the emerging intelligent rehabilitation techniques for the evaluation and treatment of motor dysfunction caused by nervous system diseases.
- Published
- 2021
- Full Text
- View/download PDF
48. Preparation, Identification, and Activity Evaluation of Eight Antioxidant Peptides from Protein Hydrolysate of Hairtail (Trichiurus japonicas) Muscle
- Author
-
Xiu-Rong Yang, Lun Zhang, Dong-Ge Ding, Chang-Feng Chi, Bin Wang, and Jian-Cong Huo
- Subjects
hairtail (Trichiurus japonicas) ,muscle ,peptide ,antioxidant activity ,Biology (General) ,QH301-705.5 - Abstract
In this report, protein of hairtail (Trichiurus japonicas) muscle was separately hydrolyzed using five kinds of proteases (alcalase, trypsin, neutrase, pepsin, and papain), and the papain- and alcalase-hydrolysates showed higher 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH•) and hydroxyl radical (HO•) scavenging activity than other three protease hydrolysates. Therefore, the protein hydrolysate of hairtail muscle (HTP) was prepared using binary-enzymes hydrolysis process (papain + alcalase). Subsequently, eight antioxidant peptides were purified from HTP using membrane ultrafiltration and chromatography technology, and their amino acid sequences were identified as Gln-Asn-Asp-Glu-Arg (TJP1), Lys-Ser (TJP2), Lys-Ala (TJP3), Ala-Lys-Gly (TJP4), Thr-Lys-Ala (TJP5), Val-Lys (TJP6), Met-Lys (TJP7), and Ile-Tyr-Gly (TJP8) with molecular weights of 660.3, 233.0, 217.1, 274.1, 318.0, 245.1, 277.0, and 351.0 Da, respectively. TJP3, TJP4, and TJP8 exhibited strong scavenging activities on DPPH• (EC50 0.902, 0.626, and 0.663 mg/mL, respectively), HO• (EC50 1.740, 2.378, and 2.498 mg/mL, respectively), superoxide anion radical (EC50 2.082, 2.538, and 1.355 mg/mL, respectively), and 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical (EC50 1.652, 0.831, and 0.586 mg/mL, respectively). Moreover, TJP3, TJP4, and TJP8 showed higher reducing power and inhibiting ability on lipid peroxidation in a linoleic acid model system. These results suggested that eight isolated peptides (TJP1 to TJP8), especially TJP3, TJP4, and TJP8 might serve as potential antioxidants applied in the pharmaceutical and health food industries.
- Published
- 2019
- Full Text
- View/download PDF
49. Correlation analysis and clinical significance of Musculoskeletal Ultrasound semi-quantitative grading with bone salt metabolism, Rheumatoid factor and Erythrocyte Sedimentation rate in patients with Rheumatoid Arthritis.
- Author
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Song PZ, Wu N, Huo C, and Wang XM
- Abstract
Objective: To determine the correlation and clinical significance of musculoskeletal ultrasound semi-quantitative grading with bone salt metabolism, rheumatoid factor and erythrocyte sedimentation rate (ESR) in patients with rheumatoid arthritis., Methods: This is a clinical comparative study. A total of 240 patients with rheumatoid arthritis admitted to Baoding NO.1 Central Hospital were selected according to the DAS28 score of rheumatoid arthritis, and were divided into four groups, with 60 cases in each group from May 2020 to May 2022. The differences and correlation of musculoskeletal ultrasound semi-quantitative grading, bone metabolism indicators, erythrocyte sedimentation rate and rheumatoid factor among the four groups were statistically analyzed., Results: The scores of bone erosion, synovial hyperplasia, joint effusion and intrasynovial blood flow in Group-H were significantly higher than those in Group-R, L and M, with statistically significant differences(p=0.00). The procollagen Type-1 N-terminal propeptide(P1NP), bone-specific alkaline phosphatase(BALP) and osteoprotegerin(OPG) in Group-H were significantly lower than those in Group-R, L and M, with statistically significant differences(p=0.00); The tartrate-resistant acid phosphatase(TRAC) in Group-H was significantly higher than that in Group-R, L and M, with a statistically significant difference(p=0.00). The levels of RF and ESR in Group-H were significantly higher than those in Group-R, L and M, with statistically significant differences(p=0.00)., Conclusion: Musculoskeletal ultrasound semi-quantitative grading is correlated with the level of bone salt metabolism, rheumatoid factor and ESR in patients with rheumatoid arthritis. It can be combined with laboratory examination to objectively judge the severity of the course of rheumatoid arthritis., Competing Interests: Declaration of conflicting interest: None., (Copyright: © Pakistan Journal of Medical Sciences.)
- Published
- 2023
- Full Text
- View/download PDF
50. [Case of bilateral diaphragm paralysis].
- Author
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Jia KX, Wang YH, and Tian CH
- Subjects
- Humans, Male, Middle Aged, Respiratory Paralysis pathology, Acupuncture Therapy, Diaphragm pathology, Respiratory Paralysis therapy
- Published
- 2011
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