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Your search keyword '"Confocal microspectroscopy"' showing total 5 results
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5 results on '"Confocal microspectroscopy"'

1. Nonlinear absorption microspectroscopy of single perylene nanocrystals with a multichannel double lock-in amplifier.

Author

Ishino, Heisuke, Iwai, Shigeto, Iwamoto, Shunji, Okumura, Tsuneo, Kobayashi, Takayoshi, and Tokunaga, Eiji

Subjects
*PERYLENE, *ABSORPTION spectra, *NANOCRYSTALS, *OPTOELECTRONIC devices, *SPECTRUM analysis, *SCIENTIFIC experimentation
Abstract

The article focuses on the experiment on nonlinear absorption microspectroscopy of single Perylene nanocrystals. It discusses the nature of Perylene, a polycyclic aromatic molecule used for optoelectric devices. It provides details of the methods of the experiment including the preparation of the samples and the apparatus used. The experiment resulted in obtaining nonlinear absorption spectra of single perylene nanocrystals and findings on the spectral width of the free Frenkel exciton band.

Published
2010
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2. Characterization of cellular chemical dynamics using combined microfluidic and Raman techniques.

Author

Xunli Zhang, Huabing Yin, Cooper, Jon M., and Haswell, Stephen J.

Subjects
*MICROFLUIDICS, *RAMAN effect, *CONFOCAL microscopy, *HAMSTERS as laboratory animals, *FLUID dynamics
Abstract

The integration of a range of technologies including microfluidics, surface-enhanced Raman scattering and confocal microspectroscopy has been successfully used to characterize in situ single living CHO (Chinese hamster ovary) cells with a high degree of spatial (in three dimensions) and temporal (1 s per spectrum) resolution. Following the introduction of a continuous flow of ionomycin, the real time spectral response from the cell was monitored during the agonist-evoked Ca2+ flux process. The methodology described has the potential to be used for the study of the cellular dynamics of a range of signalling processes. [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]

Published
2008
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4. Intracellular molecular interactions of antitumor drug amsacrine (m-AMSA) as revealed by surface-enhanced Raman spectroscopy

Author

Michel Manfait, Igor Chourpa, Jean-François Riou, and Hamid Morjani

Subjects
Topisomerase II inhibitor, Amsacrine, SER spectroscopy, Ternary cleavable complex, Stereochemistry, Biophysics, Antineoplastic Agents, Spectrum Analysis, Raman, Biochemistry, K562 cell line, chemistry.chemical_compound, Structural Biology, Tumor Cells, Cultured, Genetics, medicine, Humans, Topoisomerase II Inhibitors, Enzyme Inhibitors, Cytotoxicity, Molecular Biology, Ternary complex, chemistry.chemical_classification, Base Composition, biology, Topoisomerase, DNA, DNA, Neoplasm, Cell Biology, Confocal microspectroscopy, DNA Topoisomerases, Type II, Enzyme, chemistry, Cancer cell, Acridine, biology.protein, Plasmids, medicine.drug
Abstract

Cytotoxicity of several classes of antitumor DNA intercalators is thought to result from disturbance of DNA metabolism following trapping of the nuclear enzyme DNA topoisomerase II as a covalent complex on DNA. Here, molecular interactions of the potent antitumor drug amsacrine ( m -AMSA), an inhibitor of topoisomerase II, within living K562 cancer cells have been studied using surface-enhanced Raman (SER) spectroscopy. The work is based on data of the previously performed model SER experiments dealing with amsacrine/ DNA, drug/topoisomerase II and drug/DNA/topoisomerase II complexes in aqueous buffer solutions. The SER data indicated two kinds of amsacrine interactions in the model complexes with topoisomerase II alone or within ternary complex: non-specific (via the acridine moiety) and specific to the enzyme conformation (via the side chain of the drug). These two types of interactions have been both revealed by the micro-SER spectra of amsacrine within living K562 cancer cells. Our data suppose the specific interactions of amsacrine with topoisomerase II via the side chain of the drug (particular feature of the drug/topoisomerase II and ternary complexes) to be crucial for its inhibitory activity.

Published
1996

5. Characterization of cellular chemical dynamics using combined microfluidic and Raman techniques

Author

Xunli Zhang, Jon Cooper, Huabing Yin, and Stephen J. Haswell

Subjects
In situ, Confocal, Microfluidics, Cell Culture Techniques, Nanotechnology, CHO Cells, Gold Colloid, Spectrum Analysis, Raman, Biochemistry, Chemistry Techniques, Analytical, Analytical Chemistry, symbols.namesake, Cricetulus, Imaging, Three-Dimensional, Cricetinae, Animals, Surface-enhanced Raman scattering (SERS), Dynamic monitoring, Cells, Cultured, Chinese hamster ovary (CHO) cells, Original Paper, Chemistry, Chinese hamster ovary cell, Resolution (electron density), Equipment Design, Microfluidic Analytical Techniques, Characterization (materials science), Confocal microspectroscopy, symbols, Biophysics, Calcium, Raman spectroscopy, Raman scattering
Abstract

The integration of a range of technologies including microfluidics, surface-enhanced Raman scattering and confocal microspectroscopy has been successfully used to characterize in situ single living CHO (Chinese hamster ovary) cells with a high degree of spatial (in three dimensions) and temporal (1 s per spectrum) resolution. Following the introduction of a continuous flow of ionomycin, the real time spectral response from the cell was monitored during the agonist-evoked Ca2+ flux process. The methodology described has the potential to be used for the study of the cellular dynamics of a range of signalling processes. Figure Spectral mapping of a single CHO cell

Published
2007

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