9 results on '"Conde-Felipe M"'
Search Results
2. Potential role for mucosal IgA in modulating Haemonchus contortus adult worm infection in sheep
- Author
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Hernández, J.N., primary, Hernández, A., additional, Stear, M.J., additional, Conde-Felipe, M., additional, Rodríguez, E., additional, Piedrafita, D., additional, and González, J.F., additional
- Published
- 2016
- Full Text
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3. Correlation between Cross-Sectional Anatomy and Computed Tomography of the Normal Six-Banded Armadillo ( Euphractus sexcintus ) Nasal Cavity and Paranasal Sinuses.
- Author
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Jaber JR, Morales Bordon D, Arencibia A, Corbera JA, Conde-Felipe M, Ayala MD, and Encinoso M
- Abstract
This research aimed to study the rostral part of the head of the six-banded armadillo, applying advanced imaging techniques such as CT. Furthermore, by combining the images obtained through this technique with anatomical cross-sections, an adequate description of the structures that constitute the rostral part of the head of this species is presented. This anatomical information could provide a valuable diagnostic tool for the clinical evaluation of different disorders in the six-banded armadillo's nasal cavity and paranasal sinuses.
- Published
- 2024
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4. Metaphylactic strategies using toltrazuril against coccidiosis in goat kids.
- Author
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Guedes AC, Conde-Felipe M, Barba E, Molina JM, Del Carmen Muñoz M, Ferrer O, Martín S, Hermosilla C, Taubert A, and Ruiz A
- Subjects
- Animals, Goats, Triazines therapeutic use, Triazines pharmacology, Feces, Oocysts, Body Weight, Coccidiosis drug therapy, Coccidiosis prevention & control, Coccidiosis veterinary, Eimeria, Goat Diseases drug therapy, Goat Diseases prevention & control
- Abstract
Goat coccidiosis compromises animal welfare, reduces productivity and may cause mortality and delayed growth rates in goat kids around the weaning period worldwide. This field study was conducted to evaluate the efficacy of metaphylactic treatments with two doses of toltrazuril (20 or 40 mg/kg body weight - BW, p. o.), at different timing, in kids naturally infected with Eimeria spp. A total of 97 healthy goat kids (Majorera milk aptitude breed) were divided into five groups, depending on the age of treatment (2 or 7 weeks). One group remained untreated as a negative control until the end of the study. Faecal oocyst shedding, faecal consistency, and body weight of the animals were monitored at day 0 and at weekly intervals. Counts of oocysts per gram of faeces (OPG) were determined by a modified McMaster technique. Morphometric identification of Eimeria species was carried out on individual faecal samples from each experimental group after oocyst sporulation. Goat kids treated at two weeks of age maintained OPG values close to zero during the 5 weeks post-treatment and, overall, had lower faecal oocyst counts than untreated control animals. No significant differences were observed between the two doses of toltrazuril used in two-week-old treated animals. By contrast, when treatment was carried out at seven weeks of age, the dose of 40 mg/kg BW of toltrazuril reduced oocyst levels for longer and to a greater extent than the 20 mg/kg dose. Irrespectively of the treatment and dose, toltrazuril delayed the appearance of pathogenic Eimeria species, i. e. Eimeria ninakohlyakimovae and Eimeria arloingi. As a whole, Eimeria christenseni, with a rather moderate pathogenicity, was highly predominant throughout the study period, including the untreated control group, which was probably the reason why clinical signs of coccidiosis were barely observed throughout the experiment. Under these circumstances, the positive effect of toltrazuril on body weight condition observed in some treated groups was difficult to correlate to the timing and doses. Metaphylactic treatments with 20 mg/kg BW toltrazuril given at two weeks of age are sufficient to control oocyst excretion in goat kids; whereas if administered later in 7-week-old animals, thereby coinciding with the frequently observed peak of oocyst elimination in goat kids under field conditions, a higher dose might be advisable to prevent environmental contamination with infectious oocysts., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
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5. A Cadaveric Study Using Anatomical Cross-Section and Computed Tomography for the Coelomic Cavity in Juvenile Cory's Shearwater (Aves, Procellariidae , Calonectris borealis ).
- Author
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Morales Espino A, Déniz S, Fumero-Hernández M, Encinoso M, Calabuig P, Conde-Felipe M, and Jaber JR
- Abstract
Birds play a crucial role in ecosystems, engaging in key functions such as pollination, pest control, and seed dispersal. The anatomical understanding of these species is essential to addressing emerging challenges, including climate change and habitat loss, which directly impact their survival. Detailed knowledge of avian anatomy is fundamental for research across various disciplines, ranging from ornithology to veterinary medicine. Therefore, this study aims to disseminate the understanding of avian anatomy and the application of computed tomography (CT) for visualizing the coelomic cavity in Cory's Shearwater ( Calonectris borealis ). Recent advances in comprehending the anatomical structures of this region in avian species are highlighted, with a specific focus on Cory's Shearwater as a study model. Various anatomical cross-sections and transverse CT images were described and analyzed in detail, offering a comprehensive insight into the coelomic cavity from different perspectives. The correlation between anatomical cross-sections and CT images is emphasized as crucial for a profound understanding of avian anatomy. This research contributes to the broader knowledge of avian anatomy, with potential implications for conservation efforts and veterinary practices.
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- 2024
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6. Nurturing a Respectful Connection: Exploring the Relationship between University Educators and Students in a Spanish Veterinary Faculty.
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Ramírez AS, Jaber JR, Rosales RS, Conde-Felipe M, Rodríguez F, Corbera JA, Suárez-Pérez A, Encinoso M, and Muniesa A
- Abstract
The respect of the teacher for the student is essential for effective teaching from the perspective of the students, even in comparison to the knowledge and communication capacity of the teacher. Consequently, the optimal development of this characteristic fosters a more effective and efficient student-teacher relationship. We initiated this research following a conversation with a group of university students, who expressed their discontent regarding the lack of respect shown towards them by some teachers. Therefore, we conducted a descriptive study using online surveys, focusing on the central axis in the teacher-student relationship. The results highlighted the need for faculty members to analyze and question their attitudes towards their students. This paper presents initial results of the data collected at the Veterinary Faculty of the University of Las Palmas de Gran Canaria.
- Published
- 2023
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7. Immunization with thiol-binding proteins from Haemonchus contortus adult worms partially protects goats against infection during prepatency.
- Author
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Molina JM, Hernández YI, Ferrer O, Conde-Felipe MM, Rodríguez F, and Ruiz A
- Subjects
- Animals, Goats, Immunization, Vaccination veterinary, Eosinophils, Feces parasitology, Parasite Egg Count veterinary, Haemonchus, Haemonchiasis prevention & control, Haemonchiasis veterinary, Goat Diseases parasitology
- Abstract
To contribute of the knowledge of the immune mechanisms underlying the response to the immunization of goats with thiol-binding proteins fractions (PBS-TSBP) from Haemonchus contortus (H. contortus) adult worms, this study analyzed the degree of protection and the immune responses developed against the parasite after vaccination with this antigenic complex during the time-elapsing between challenge with L3 of the parasite and the development of adult worms, evidenced by the appearance of first faecal eggs (prepatent period or prepatency). Goat kids immunized with PBS-TBSP generated an immune response during the prepatency which translates into a reduction in the number of worms, as well as a lower reduction on packed cell volume and plasma protein levels in relation to the non-vaccinated animals. As previously described in other studies carried out after the prepatent period, this protection was associated with a systemic humoral response. At the local level, a specific humoral response was also observed, together with an immune-inflammatory infiltrate in the gastric mucosa of MCH-II + cells and CD4
+ lymphocytes, whose number was associated with a reduction in the number of worms and an increase in plasma proteins. A high peripheral eosinophilia was detected, but no corresponding increased infiltration of the gastric mucosa by eosinophils or globular leukocytes was observed. In agreement with previous data on the immunolocalization of the antigens used here, the results obtained contribute to the idea that these may be excretion/secretion (E/S) products necessary for parasite survival, whose inactivation during the larval and/or pre-adult stages may have contributed to immunoprotection., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2023
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8. Susceptibility of Malassezia pachydermatis to aminoglycosides.
- Author
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Silva FA, Conde-Felipe M, Rosario I, Ferrer O, Real F, Déniz S, Acosta F, Padilla D, and Acosta-Hernández B
- Subjects
- Amikacin analysis, Amikacin pharmacology, Aminoglycosides analysis, Gentamicins analysis, Gentamicins pharmacology, Malassezia growth & development, Netilmicin analysis, Netilmicin pharmacology, Tobramycin analysis, Tobramycin pharmacology, Aminoglycosides pharmacology, Antifungal Agents pharmacology, Malassezia drug effects, Microbial Sensitivity Tests methods
- Abstract
Previous studies have evaluated the action of gentamicin against Malassezia pachydermatis. The aim of this study was to evaluate in vitro susceptibility of M. pachydermatis to the aminoglycosides- gentamicin, tobramycin, netilmicin and framycetin. The minimum inhibitory concentration (MIC) of gentamicin was determined following methods M27-A3 microdilution and Etest
® . The Etest® was used to determine the minimum inhibitory concentration (MIC) of the tobramycin and netilmicin. The Kirby-Bauer test was used to determine the antibiotic susceptibility to the framycetin. The MIC50 and MIC90 were 8.12 μg/mL and 32.5 μg/mL by microdilution method for gentamicin. The MIC50, determined by the Etest® , was 8 μg/mL for gentamicin and netilmicin and 64 μg/mL for tobramycin. The MIC90 was 16 and 32 μg/mL for gentamicin and netilmicin respectively. The MIC90 was outside of the detectable limits for tobramycin. To framycetin, 28 strains (40%) of the 70 M. pachydermatis isolates tested showed a diameter of 22 mm, 22 strains (31.42%) showed a diameter of 20 mm, 16 strains showed a diameter of ≤ 18 mm, and only 5.71% of the isolates showed a diameter of ≥ 22 mm. This study provides evidence of high in vitro activity of the aminoglycosides-gentamicin, tobramycin, netilmicin and framycetin against M. pachydermatis. For gentamicin Etest® showed similar values of MIC50 y MIC90 that the obtained by microdilution method. We considered Etest® method could be a good method for these calculations with aminoglycosides., (© 2017 Blackwell Verlag GmbH.)- Published
- 2017
- Full Text
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9. In vitro susceptibility testing of Malassezia pachydermatis to gentamicin.
- Author
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Silva FA, Ferrer O, Déniz S, Rosario I, Conde-Felipe M, Díaz EL, and Acosta-Hernández B
- Subjects
- Dose-Response Relationship, Drug, Drug Resistance, Fungal, In Vitro Techniques, Microbial Sensitivity Tests, Antifungal Agents pharmacology, Gentamicins pharmacology, Malassezia drug effects
- Abstract
Background: Two studies have observed that growth media containing gentamicin can inhibit the growth of the yeast organism Malassezia pachydermatis. The minimum inhibitory concentration (MIC) of this bactericidal antibiotic for this organism has not been previously determined., Objective: To evaluate the susceptibility of M. pachydermatis isolates to gentamicin., Methods: The MIC of gentamicin was determined using a modified version of the M27-A3 microdilution method following the guidelines of the Clinical and Laboratory Standards Institute. A modified Christensen's urea broth was used to enhance the growth of the M. pachydermatis isolates. Visual and spectrophotometric end-point readings were performed to detect the presence or absence of yeast growth., Results: The MIC50 and MIC90 of gentamicin were 8.12 μg/mL and 32.5 μg/mL, respectively; M. pachydermatis strains were classified as susceptible (S), intermediate (I) and resistant (R). The susceptibility of these isolates to gentamicin in vitro, by visual and spectrophotometric end-point reading, was: S, 54-56%; I, 40-41%; and R, 3-6%., Conclusion: Prospective MICs for M. pachydermatis have been established for gentamicin., (© 2017 ESVD and ACVD.)
- Published
- 2017
- Full Text
- View/download PDF
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