115 results on '"Compagnone, M"'
Search Results
2. A first-in-human trial on the safety and immunogenicity of COVID-eVax, a cellular response-skewed DNA vaccine against COVID-19
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Aurisicchio, L, Brambilla, N, Cazzaniga, M, Bonfanti, P, Milleri, S, Ascierto, P, Capici, S, Vitalini, C, Girolami, F, Giacovelli, G, Caselli, G, Visintin, M, Fanti, F, Ghirri, M, Conforti, A, Compagnone, M, Lione, L, Salvatori, E, Pinto, E, Muzi, A, Marra, E, Palombo, F, Roscilli, G, Manenti, A, Montomoli, E, Cadossi, M, Rovati, L, Aurisicchio, Luigi, Brambilla, Nadia, Cazzaniga, Marina E, Bonfanti, Paolo, Milleri, Stefano, Ascierto, Paolo A, Capici, Serena, Vitalini, Cristina, Girolami, Federica, Giacovelli, Giampaolo, Caselli, Gianfranco, Visintin, Michela, Fanti, Francesca, Ghirri, Matteo, Conforti, Antonella, Compagnone, Mirco, Lione, Lucia, Salvatori, Erika, Pinto, Eleonora, Muzi, Alessia, Marra, Emanuele, Palombo, Fabio, Roscilli, Giuseppe, Manenti, Alessandro, Montomoli, Emanuele, Cadossi, Matteo, Rovati, Lucio C, Aurisicchio, L, Brambilla, N, Cazzaniga, M, Bonfanti, P, Milleri, S, Ascierto, P, Capici, S, Vitalini, C, Girolami, F, Giacovelli, G, Caselli, G, Visintin, M, Fanti, F, Ghirri, M, Conforti, A, Compagnone, M, Lione, L, Salvatori, E, Pinto, E, Muzi, A, Marra, E, Palombo, F, Roscilli, G, Manenti, A, Montomoli, E, Cadossi, M, Rovati, L, Aurisicchio, Luigi, Brambilla, Nadia, Cazzaniga, Marina E, Bonfanti, Paolo, Milleri, Stefano, Ascierto, Paolo A, Capici, Serena, Vitalini, Cristina, Girolami, Federica, Giacovelli, Giampaolo, Caselli, Gianfranco, Visintin, Michela, Fanti, Francesca, Ghirri, Matteo, Conforti, Antonella, Compagnone, Mirco, Lione, Lucia, Salvatori, Erika, Pinto, Eleonora, Muzi, Alessia, Marra, Emanuele, Palombo, Fabio, Roscilli, Giuseppe, Manenti, Alessandro, Montomoli, Emanuele, Cadossi, Matteo, and Rovati, Lucio C
- Abstract
The COVID-19 pandemic and the need for additional safe, effective, and affordable vaccines gave new impetus into development of vaccine genetic platforms. Here we report the findings from the phase 1, first-in-human, dose-escalation study of COVID-eVax, a DNA vaccine encoding the receptor binding domain (RBD) of the SARS-CoV-2 spike protein. Sixty-eight healthy adults received two doses of 0.5, 1, or 2 mg 28 days apart, or a single 2-mg dose, via intramuscular injection followed by electroporation, and they were monitored for 6 months. All participants completed the primary safety and immunogenicity assessments after 8 weeks. COVID-eVax was well tolerated, with mainly mild to moderate solicited adverse events (tenderness, pain, bruising, headache, and malaise/fatigue), less frequent after the second dose, and it induced an immune response (binding antibodies and/or T cells) at all prime-boost doses tested in up to 90% of the volunteers at the highest dose. However, the vaccine did not induce neutralizing antibodies, while particularly relevant was the T cell-mediated immunity, with a robust Th1 response. This T cell-skewed immunological response adds significant information to the DNA vaccine platform and should be assessed in further studies for its protective capacity and potential usefulness also in other therapeutic areas, such as oncology.
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- 2023
3. Male accessory gland infection: diagnosis and treatment
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Cannarella, R., Condorelli, R. A., Cimino, L., Mongioi, L. M., Compagnone, M., Barbagallo, F., Crafa, A., Calogero, A. E., and La Vignera, S.
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Antibiotics ,Oxidative stress ,Infertility ,Male accessory gland infection ,Antioxidants - Published
- 2023
4. p63 controls cell migration and invasion by transcriptional regulation of MTSS1
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Giacobbe, A, Compagnone, M, Bongiorno-Borbone, L, Antonov, A, Markert, E K, Zhou, J H, Annicchiarico-Petruzzelli, M, Melino, G, and Peschiaroli, A
- Published
- 2016
- Full Text
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5. P58 IMMEDIATE CORONARY ANGIOGRAPHY AND SYSTEMATIC TARGETED TEMPERATURE MANAGEMENT ARE ASSOCIATED WITH IMPROVED OUTCOME IN COMATOSE SURVIVORS OF CARDIAC ARREST
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Dall’Ara, G, primary, Compagnone, M, additional, Spartà, D, additional, Carletti, R, additional, Grotti, S, additional, Guerrieri, G, additional, Gaetani, S, additional, Cortigiani, M, additional, Maitan, S, additional, Fabbri, A, additional, Ottani, F, additional, Caravita, L, additional, Tarantino, F, additional, and Galvani, M, additional
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- 2022
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6. 100.38 Sheathless Radial Approach in Coronary Rotational Atherectomy: Data From Two High-Volume Centers
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Dall'Ara, G., Micari, A., Alagna, G., Spartà, D., Compagnone, M., Grotti, S., Guerrieri, G., Campanella, F., Taverna, G., Galvani, M., Tarantino, F., and Andò, G.
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- 2024
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7. P08.03 Neoantigen cancer vaccine auguments anti CTLA-4 efficacy
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Palombo, F, primary, Salvatori, E, additional, Lione, L, additional, Compagnone, M, additional, Conforti, A, additional, and Aurisicchio, L, additional
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- 2021
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8. Structural and functional basis for pan-CoV fusion inhibitors against SARS-CoV-2 and its variants with preclinical evaluation. COVID-eVax, an electroporated plasmid DNA vaccine candidate encoding the SARS-CoV-2 Receptor Binding Domain, elicits protective immune responses in animal models of COVID-19
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Conforti A, Marra E, Palombo F, Roscilli G, Ravà M, Fumagalli V, Muzi A, Maffei M, Luberto L, Lione L, Salvatori E, Compagnone M, Pinto E, Pavoni E, Bucci F, Vitagliano G, Stoppoloni D, Pacello ML, Cappelletti M, Ferrara FF, D'Acunto E, Chiarini V, Arriga R, Nyska A, Di Lucia P, Marotta D, Bono E, Giustini L, Sala E, Perucchini C, Paterson J, Ryan KA, Challis AR, Matusali G, Colavita F, Caselli G
- Published
- 2021
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9. Nutlin-3a enhances natural killer cell-mediated killing of neuroblastoma by restoring p53-dependent expression of ligands for NKG2D and DNAM-1 receptors
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Veneziani, I., Infante, P., Ferretti, E., Melaiu, O., Battistelli, C., Lucarini, V., Compagnone, M., Nicoletti, C., Castellano, A., Petrini, S., Ognibene, M., Pezzolo, A., Di Marcotullio, L., Bei, R., Moretta, L., Pistoia, V., Fruci, D., Barnaba, V., Locatelli, Franco, Cifaldi, L., Locatelli F. (ORCID:0000-0002-7976-3654), Veneziani, I., Infante, P., Ferretti, E., Melaiu, O., Battistelli, C., Lucarini, V., Compagnone, M., Nicoletti, C., Castellano, A., Petrini, S., Ognibene, M., Pezzolo, A., Di Marcotullio, L., Bei, R., Moretta, L., Pistoia, V., Fruci, D., Barnaba, V., Locatelli, Franco, Cifaldi, L., and Locatelli F. (ORCID:0000-0002-7976-3654)
- Abstract
In this study, we explored whether Nutlin-3a, a well-known, nontoxic small-molecule compound antagonizing the inhibitory interaction of MDM2 with the tumor suppressor p53, may restore ligands for natural killer (NK) cell-activating receptors (NK-AR) on neuroblastoma cells to enhance the NK cell-mediated killing. Neuroblastoma cell lines were treated with Nutlin-3a, and the expression of ligands for NKG2D and DNAM-1 NK-ARs and the neuroblastoma susceptibility to NK cells were evaluated. Adoptive transfer of human NK cells in a xenograft neuroblastoma-bearing NSG murine model was assessed. Two data sets of neuroblastoma patients were explored to correlate p53 expression with ligand expression. Luciferase assays and chromatin immunoprecipitation analysis of p53 functional binding on PVR promoter were performed. Primary neuroblastoma cells were also treated with Nutlin-3a, and neuroblastoma spheroids obtained from one high-risk patient were assayed for NK-cell cytotoxicity. We provide evidence showing that the Nutlin-3a-dependent rescue of p53 function in neuroblastoma cells resulted in (i) increased surface expression of ligands for NK-ARs, thus rendering neuroblastoma cell lines significantly more susceptible to NK cell-mediated killing; (ii) shrinkage of human neuroblastoma tumor masses that correlated with overall survival upon adoptive transfer of NK cells in neuroblastoma-bearing mice; (iii) and increased expression of ligands in primary neuroblastoma cells and boosting of NK cell-mediated disaggregation of neuroblastoma spheroids. We also found that p53 was a direct transcription factor regulating the expression of PVR ligand recognized by DNAM-1. Our findings demonstrated an immunomodulatory role of Nutlin-3a, which might be prospectively used for a novel NK cell-based immunotherapy for neuroblastoma.
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- 2021
10. Resting leukocyte telomerase activity is elevated in major depression and predicts treatment response
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Wolkowitz, O M, Mellon, S H, Epel, E S, Lin, J, Reus, V I, Rosser, R, Burke, H, Compagnone, M, Nelson, J C, Dhabhar, F S, and Blackburn, E H
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- 2012
- Full Text
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11. Coronary artery disease and reasonably incomplete coronary revascularization in high-risk patients undergoing transcatheter aortic valve implantation
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Saia F., Palmerini T., Compagnone M., Battistini P., Moretti C., Taglieri N., Bruno A. G., Ghetti G., Corsini A., Bacchi Reggiani M. -L., Marrozzini C., Rapezzi C., Marcelli C., PALMERINI, TULLIO, Policlinico S. Orsola-malpighi, Alma Mater Studiorum Università di Bologna [Bologna] (UNIBO)-Servizio sanitario regionale Emilia-Romagna, Alma Mater Studiorum Università di Bologna [Bologna] (UNIBO), Mobilités : Vieillissement, Pathologie, Santé (COMETE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Saia F., Palmerini T., Compagnone M., Battistini P., Moretti C., Taglieri N., Marcelli C., Bruno A.G., Ghetti G., Corsini A., Bacchi Reggiani M.-L., Marrozzini C., and Rapezzi C.
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Male ,Time Factors ,coronary artery disease ,heart team ,revascularization ,transcatheter aortic valve replacement ,Databases, Factual ,medicine.medical_treatment ,[SDV]Life Sciences [q-bio] ,[SHS.PSY]Humanities and Social Sciences/Psychology ,Coronary Artery Disease ,030204 cardiovascular system & hematology ,Coronary Angiography ,Ventricular Function, Left ,Coronary artery disease ,0302 clinical medicine ,Risk Factors ,Myocardial Revascularization ,Prevalence ,030212 general & internal medicine ,Hospital Mortality ,Registries ,Aged, 80 and over ,Ejection fraction ,Incidence (epidemiology) ,General Medicine ,3. Good health ,Prosthesis Failure ,Treatment Outcome ,Aortic Valve ,Heart Valve Prosthesis ,Cardiology ,Female ,Cardiology and Cardiovascular Medicine ,Acute coronary syndrome ,medicine.medical_specialty ,Transcatheter aortic ,Revascularization ,Risk Assessment ,NO ,Transcatheter Aortic Valve Replacement ,03 medical and health sciences ,Internal medicine ,Heart team ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,cardiovascular diseases ,Acute Coronary Syndrome ,Aged ,Retrospective Studies ,business.industry ,[SCCO.NEUR]Cognitive science/Neuroscience ,Stroke Volume ,Aortic Valve Stenosis ,medicine.disease ,Coronary revascularization ,business - Abstract
International audience; ObjectivesTo evaluate the long-term impact of coronary artery disease (CAD) and heart team-guided incomplete coronary revascularization in patients undergoing transcatheter aortic valve implantation (TAVI).BackgroundRevascularization strategy of CAD diagnosed with routine coronary angiography before TAVI is uncertain.MethodsFive hundred and forty consecutive TAVI patients were classified as having CAD or normal coronary arteries (no-CAD). Within the CAD group, patients were further classified as those with complete (CR) versus incomplete revascularization (IR). Revascularization strategy was guided by the Heart Team following an algorithm largely based on current guidelines. Main outcome of interest was the incidence of 5-year cardiovascular (CV) death.ResultsPrevalence of CAD was 53.9%. CAD patients showed significantly lower left ventricular ejection fraction (LVEF: 55.8 ± 13.4% CAD vs. 61.4% ± 12.1 no-CAD, p
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- 2018
12. Impact on clinical outcomes of right ventricular response to percutaneous correction of secondary mitral regurgitation
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Caiffa, T, primary, Castrichini, M, additional, Biagini, E, additional, De Luca, A, additional, Compagnone, M, additional, Berardini, A, additional, Merlo, M, additional, Fabris, E, additional, Vitrella, G, additional, Pinamonti, B, additional, Korcova, R, additional, Barbati, G, additional, Saia, F, additional, Stolfo, D, additional, and Sinagra, G, additional
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- 2021
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13. Derivation and validation of a scoring system to predict after discharge risk of cardiac events in patients with acute myocardial infarction undergoing percutaneous coronary revascularization
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Corsini, A, primary, Massarelli, G, additional, Bruno, M, additional, Bruno, A.G, additional, Compagnone, M, additional, Ghetti, G, additional, Saia, F, additional, Galie', N, additional, and Taglieri, N, additional
- Published
- 2020
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14. 3 Impact of morbid obesity and obesity phenotype on outcomes post transcatheter aortic valve replacement
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McInerney, A, primary, Tirado-Conte, G, additional, Rodes-Cabau, J, additional, Campelo-Parada, F, additional, Tafur Soto, JD, additional, Barbanti, M, additional, Muñoz-Garcia, E, additional, Arif, M, additional, Lopez, D, additional, Toggweiler, S, additional, Veiga, G, additional, Pylko, A, additional, Sevilla, T, additional, Compagnone, M, additional, Regueiro, A, additional, Serra, V, additional, Carnero, M, additional, Oteo, JF, additional, Rivero, F, additional, Barbosa Ribeiro, H, additional, Guimaraes, L, additional, Matta, A, additional, Giraldo Echavarria, N, additional, Valvo, R, additional, Moccetti, F, additional, Muñoz-Garcia, AJ, additional, Lopez-Pais, J, additional, Garcia del Blanco, B, additional, Carter Campanha Borges, D, additional, Gonzalo, N, additional, Dumont, E, additional, Criscione, E, additional, Dabrowski, M, additional, Alfonso, F, additional, de la Torre Hernández, JM, additional, Cheema, AN, additional, Amat-Santos, I, additional, Saia, F, additional, Escaned, J, additional, and Nombela-Franco, L, additional
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- 2020
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15. Patients with childhood cancer who underwent thoracic or abdominal irradiation have poor gonadal function in adulthood
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Duca, Y, Di Cataldo, A, Russo, G, Cannata, E, Alamo, A, Burgio, G, Compagnone, M, Condorelli, Ra, La Vignera, S, and Calogero, Ae
- Published
- 2019
16. DETERMINATION OF VOLATILE COMPOUNDS IN CANNABIS SATIVA L. USING ELECTRONIC NOSE BASED ON PEPTIDE AND HAIRPIN DNA Vs. SOLID-PHASE MICROEXTRACTION AND GC-MS
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Gaggiotti, S., Palmieri, S., Della Pelle, F., Ricci, A., Lo Sterzo, C., and Compagnone, M. Mascini and D.
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- 2019
17. Influenza della patologie sistemiche ed endocrine sulla fertilità maschile
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Calogero, Ae, Condorelli, ROSITA ANGELA, Cannarella, R, Alamo, A, Cimino, L, Compagnone, M, and La Vignera, S
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- 2018
18. Hydrocortisone dual-release formulation: a new therapeutic strategy in adrenal insufficiency
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Mongioi', LAURA MARIA, Condorelli, ROSITA ANGELA, Cimino, Laura, Compagnone, M., Burgio, G., LA VIGNERA, SANDRO SALVUCCIO MARIA, and Calogero, Aldo Eugenio
- Published
- 2017
19. SHEATHLESS RADIAL APPROACH IN CONTEMPORARY CORONARY ROTATIONAL ATHERECTOMY: DATA FROM TWO HIGH VOLUME CENTERS
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Dall‘Ara, G, Alagna, G, Spartà, D, Compagnone, M, Grotti, S, Guerrieri, G, Campanella, F, Taverna, G, Galvani, M, Tarantino, F, and Andò, G
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- 2024
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20. Effects of robertsonian translocation 13;14 on biofunctional sperm parameters
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Mongioì, Lm, Condorelli, Ra, Compagnone, M, Lanzafame, F, LA VIGNERA, SANDRO SALVUCCIO MARIA, and Calogero, Aldo Eugenio
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- 2016
21. ΔNp63α modulates histone methyl transferase SETDB1 to transcriptionally repress target genes in cancers
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Regina, C, primary, Compagnone, M, additional, Peschiaroli, A, additional, Lena, AM, additional, Melino, G, additional, and Candi, E, additional
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- 2016
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22. Anti-tumoral effect of desmethylclomipramine in lung cancer stem cells
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Bongiorno-Borbone, L, Giacobbe, A, Compagnone, M, Eramo, A, De Maria Marchiano, Ruggero, Peschiaroli, A, Melino, G., De Maria Marchiano R (ORCID:0000-0003-2255-0583), Bongiorno-Borbone, L, Giacobbe, A, Compagnone, M, Eramo, A, De Maria Marchiano, Ruggero, Peschiaroli, A, Melino, G., and De Maria Marchiano R (ORCID:0000-0003-2255-0583)
- Abstract
Lung cancer is the most feared of all cancers because of its heterogeneity and resistance to available treatments. Cancer stem cells (CSCs) are the cell population responsible for lung cancer chemoresistance and are a very good model for testing new targeted therapies. Clomipramine is an FDA-approved antidepressant drug, able to inhibit in vitro the E3 ubiquitin ligase Itch and potentiate the pro-apoptotic effects of DNA damaging induced agents in several cancer cell lines. Here, we investigated the potential therapeutic effect of desmethylclomipramine (DCMI), the active metabolite of Clomipramine, on the CSCs homeostasis. We show that DCMI inhibits lung CSCs growth, decreases their stemness potential and increases the cytotoxic effect of conventional chemotherapeutic drugs. Being DCMI an inhibitor of the E3 ubiquitin ligase Itch, we also veri ed the effect of Itch deregulation on CSCs survival. We found that the siRNA-mediated depletion of Itch induces similar anti-proliferative effects on lung CSCs, suggesting that DCMI might exert its effect, at least in part, by inhibiting Itch. Notably, Itch expression is a negative prognostic factor in two primary lung tumors datasets, supporting the potential clinical relevance of Itch inhibition to circumvent drug resistance in the treatment of lung cancer.
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- 2015
23. p63 controls cell migration and invasion by transcriptional regulation of MTSS1
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Giacobbe, A, primary, Compagnone, M, additional, Bongiorno-Borbone, L, additional, Antonov, A, additional, Markert, E K, additional, Zhou, J H, additional, Annicchiarico-Petruzzelli, M, additional, Melino, G, additional, and Peschiaroli, A, additional
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- 2015
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24. Resting leukocyte telomerase activity is elevated in major depression and predicts treatment response
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Wolkowitz, O M, primary, Mellon, S H, additional, Epel, E S, additional, Lin, J, additional, Reus, V I, additional, Rosser, R, additional, Burke, H, additional, Compagnone, M, additional, Nelson, J C, additional, Dhabhar, F S, additional, and Blackburn, E H, additional
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- 2011
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25. BRAF p.Val600Glu (V600E) somatic mutation is mainly associated with MSS phenotype in metastatic colorectal cancer
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Qiu, J. H., Compagnone, M., Laibe, S., arnaud lagarde, Goncalves, A., Turrini, O., Xerri, L., Monges, G., and Olschwang, S.
26. Sheathless Radial Approach in Coronary Rotational Atherectomy: Data From Two High-Volume Centers.
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Dall'Ara, G., Micari, A., Alagna, G., Spartà, D., Compagnone, M., Grotti, S., Guerrieri, G., Campanella, F., Taverna, G., Galvani, M., Tarantino, F., and Andò, G.
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ATHERECTOMY - Published
- 2024
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27. Long-term outcome of prosthesis-patient mismatch after transcatheter aortic valve replacement
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Maria Letizia Bacchi Reggiani, Antonio Bruno, Mateusz Orzalkiewicz, Giulia Marchetti, Nazzareno Galiè, Nevio Taglieri, Francesco Saia, Miriam Compagnone, Tullio Palmerini, Cinzia Marrozzini, Gabriele Ghetti, Compagnone M., Marchetti G., Taglieri N., Ghetti G., Bruno A.G., Orzalkiewicz M., Marrozzini C., Bacchi Reggiani M.-L., Palmerini T., Galie N., and Saia F.
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medicine.medical_specialty ,Aortic valve stenosi ,Degenerated aortic valve bioprosthesi ,medicine.medical_treatment ,030204 cardiovascular system & hematology ,Prosthesis Design ,Transcatheter Aortic Valve Replacement ,03 medical and health sciences ,0302 clinical medicine ,Valve replacement ,Risk Factors ,Internal medicine ,medicine ,Humans ,030212 general & internal medicine ,Myocardial infarction ,Risk factor ,Stroke ,Bioprosthesis ,Heart Valve Prosthesis Implantation ,business.industry ,Incidence (epidemiology) ,Prosthesis-patient mismatch ,Aortic Valve Stenosis ,medicine.disease ,Confidence interval ,Treatment Outcome ,Aortic Valve ,Heart Valve Prosthesis ,Aortic valve stenosis ,Cardiology ,Cardiology and Cardiovascular Medicine ,business ,Body mass index - Abstract
Background Incidence and long-term clinical consequences of prosthesis-patient mismatch (PPM) after transcatheter aortic valve replacement (TAVR) are still unclear. Methods We enrolled 710 consecutive patients who underwent TAVR. PPM was defined as absent if the index orifice area (iEOA) was >0.85 cm2/m2, moderate if the iEOA was between 0.65 and 0.85 cm2/m2 or severe if the iEOA was Results Among the 566 patients fulfilling the study criteria, the distribution of PPM was as follows: 50.5% none (n = 286), 43% moderate PPM (n = 243) and 6.5% severe PPM (n = 37). At 5-year follow-up, patients with severe PPM had a significantly higher incidence of the combined endpoint of cardiovascular death, acute myocardial infarction and stroke (p = .025) compared with the other patients. After adjusting the results for possible confounders, severe PPM remained an independent predictor of long-term adverse outcome (HR: 2.46; 95% Confidence Interval: 1.10–5.53). The independent predictors of severe PPM were valve-in-valve procedure and body mass index. Balloon-expandable valves were not associated with higher rates of severe PPM in comparison with self-expandable valves (8% vs. 5%, respectively, p = .245). Conclusions In our study severe PPM emerged as a risk factor for long-term major adverse cardiac and cerebrovascular events.
- Published
- 2020
28. Impact of Elective, Uncomplicated Target Lesion Revascularization on Cardiac Mortality After Elective Percutaneous Coronary Intervention of Unprotected Left Main Coronary Artery Disease
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Francesco Saia, Mihail Celeski, Mateusz Orzalkiewicz, Antonio Bruno, Nazzareno Galiè, Nevio Taglieri, Cinzia Marrozzini, Miriam Compagnone, Elena Nardi, Tullio Palmerini, Gabriele Ghetti, Maria Letizia Bacchi Reggiani, Compagnone M., Taglieri N., Celeski M., Ghetti G., Marrozzini C., Reggiani M.-L.B., Nardi E., Orzalkiewicz M., Bruno A.G., Galie N., Saia F., and Palmerini T.
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Male ,Reoperation ,medicine.medical_specialty ,Heart Diseases ,Prognosi ,medicine.medical_treatment ,Myocardial Infarction ,Coronary Artery Disease ,030204 cardiovascular system & hematology ,03 medical and health sciences ,Percutaneous Coronary Intervention ,0302 clinical medicine ,Internal medicine ,Myocardial Revascularization ,medicine ,Clinical endpoint ,Humans ,030212 general & internal medicine ,Myocardial infarction ,Stroke ,Aged ,Proportional Hazards Models ,Aged, 80 and over ,Elective Surgical Procedure ,business.industry ,Cardiogenic shock ,Hazard ratio ,Percutaneous coronary intervention ,Middle Aged ,Prognosis ,medicine.disease ,Heart Disease ,medicine.anatomical_structure ,Italy ,Elective Surgical Procedures ,Conventional PCI ,Proportional Hazards Model ,Cardiology ,Female ,Cardiology and Cardiovascular Medicine ,business ,Human ,Artery - Abstract
This study sought to investigate the impact of elective, uncomplicated target lesion revascularization (TLR) on long-term cardiac mortality after percutaneous coronary intervention (PCI) of unprotected left main coronary artery (ULMCA) disease. Consecutive patients undergoing PCI for ULMCA disease between January 2003 and December 2015 in 1 interventional center in Northern Italy were included. Patients presenting with cardiogenic shock, ST-segment elevation myocardial infarction (MI), as well as those undergoing urgent or complicated TLR were excluded. The primary endpoint of the study was cardiac mortality. Among the 418 patients fulfilling the study criteria, 79 (18.46%) underwent elective, uncomplicated TLR. After a median follow-up of 5.5 years, there were 23 cardiac deaths among patients undergoing elective, uncomplicated TLR versus 50 in patients not undergoing TLR. After adjusting for possible confounders, TLR was an independent predictor of cardiac mortality (Hazard ratio [HZ] = 1.92, 95% confidence interval [CI]: 1.05 to 3.49; p = 0.03). Patients undergoing TLR had also significantly higher rates of the composite of cardiac death, MI and stroke compared with the no TLR group (adjusted HR = 1.76, 95% CI 1.14 to 2.72). In conclusion, elective, uncomplicated TLR after PCI of ULMCA disease is associated with increased risk of long-term cardiac mortality. Reducing the risk of TLR after PCI of ULMCA disease may potentially improve the survival of these patients.
- Published
- 2020
29. Enhancing hydrogen production from winery wastewater through fermentative microbial culture selection
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Grazia Policastro, Federica Carraturo, Mariacristina Compagnone, Marco Guida, Massimiliano Fabbricino, Policastro, G., Carraturo, F., Compagnone, M., Guida, M., and Fabbricino, M.
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Co-fermentation ,Environmental Engineering ,Renewable Energy, Sustainability and the Environment ,Open mixed culture ,Bioengineering ,Photo fermentation ,Waste Management and Disposal ,Biorefinery ,Dark fermentation - Abstract
The present paper aims at enhancing the fermentative/co-fermentative hydrogen production from winery wastewater. Winery wastewater is a very abundant waste stream, with a high hydrogen production potential. On the other hand, it might exert toxic effects towards many hydrogen-producing bacteria. To optimize the process, a mixed microbial consortium was subject to microbial analysis to identify hydrogen-producing strains. These latter were isolated to obtain several pure cultures, either dark-fermentative, or photo-fermentative. Comparative fermentation tests were performed, using the isolated pure cultures, the initial mixed culture and a co-culture consisting of the two most performant dark fermentative and photo fermentative strains (i.e. Klebsiella pneumoniae strain MF101 and Rhodopseudomonas sp. strain BR0Y6). Results show that the initial mixed culture led to the highest production of 290 NmLH2 L−1. Further analyses on metabolic intermediates demonstrate the importance of synergies established in open fermentations to enhance the conversion of complex organic substrates to hydrogen.
- Published
- 2022
30. Impact on clinical outcomes of right ventricular response to percutaneous correction of secondary mitral regurgitation
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Laura Lupi, Thomas Caiffa, Giulia Barbati, Marianna Adamo, Davide Stolfo, Roberto Arosio, Antonio Popolo Rubbio, Enrico Fabris, Alessandra Berardini, Mattia Di Pasquale, Elena Biagini, Antonio De Luca, Miriam Compagnone, Daniela Tomasoni, Gianfranco Sinagra, Marco Merlo, Matteo Castrichini, Francesco Bedogni, Maurizio Tusa, Andrea Perkan, Francesco Saia, Caiffa, T., De Luca, A., Biagini, E., Lupi, L., Bedogni, F., Castrichini, M., Compagnone, M., Tusa, M., Berardini, A., Merlo, M., Fabris, E., Popolo Rubbio, A., Tomasoni, D., Di Pasquale, M., Arosio, R., Perkan, A., Barbati, G., Saia, F., Adamo, M., Stolfo, D., and Sinagra, G.
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medicine.medical_specialty ,Percutaneous mitral valve repair ,medicine.medical_treatment ,Left ,Ventricular Function, Left ,Interquartile range ,Internal medicine ,Clinical endpoint ,80 and over ,Medicine ,Humans ,Ventricular Function ,Heart failure with reduced ejection fraction ,Mitral regurgitation ,Right ventricular function ,Aged ,Aged, 80 and over ,Middle Aged ,Stroke Volume ,Treatment Outcome ,Cardiac Surgical Procedures ,Heart Failure ,Mitral Valve Insufficiency ,Heart transplantation ,Ejection fraction ,business.industry ,medicine.disease ,medicine.anatomical_structure ,Ventricle ,Heart failure ,Cardiology ,Cardiology and Cardiovascular Medicine ,business ,Percutaneous Mitral Valve Repair - Abstract
Aims: In patients with heart failure and reduced ejection fraction (HFrEF) and secondary mitral regurgitation (SMR), impaired right ventricular function (RVF) may negatively influence the prognosis. Percutaneous mitral valve repair (pMVR) can promote the recovery of RVF. We sought to characterize the response of the right ventricle to pMVR in HFrEF with SMR and to assess the association between improved RVF after pMVR and outcomes. Methods and results: Overall, 221 patients with HFrEF and SMR ≥3+ successfully treated with pMVR in four tertiary care centres for heart failure were included. Improved RVF was defined as Δ right ventricular fractional area change (ΔRVFAC) ≥5% at early follow-up (median time 4 months). The primary endpoint was a composite of death/heart transplantation (D/HT). Mean age was 69 ± 11 years, mean left ventricular ejection fraction was 31 ± 8% and mean RVFAC was 34 ± 9%. ΔRVFAC ≥5% occurred in 88 patients (40%) and was independent of the measures of left ventricular reverse remodelling. During a median follow-up of 29 months (interquartile range 12–46), 81 patients (37%) reached the primary endpoint. After adjustment for other significant covariates, ΔRVFAC ≥5% was significantly associated with lower risk of D/HT (hazard ratio 0.52, 95% confidence interval 0.29–0.94, P = 0.030). In the secondary outcome analysis exploring the risk of heart failure hospitalizations, ΔRVFAC ≥5% confirmed the prognostic association with the endpoint. Conclusions: In patients with HFrEF and SMR, about 40% of patients improved RVF after pMVR. RVF improvement was associated with better long-term survival free from HT and lower risk of heart failure hospitalization.
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- 2021
31. Evaluation of Microbial Communities of Bottled Mineral Waters and Preliminary Traceability Analysis Using NGS Microbial Fingerprints
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Carraturo, Federica, Del Giudice, Carmela, Compagnone, María Cristina, Libralato, Giovanni, Toscanesi, Maria, Trifuoggi, Marco, Galdiero, Emilia, Guida, Marco, Ingeniería Química y Tecnología de Alimentos, Carraturo, F., Giudice, C. D., Compagnone, M., Libralato, G., Toscanesi, M., Trifuoggi, M., Galdiero, E., and Guida, M.
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Traceability ,media_common.quotation_subject ,Geography, Planning and Development ,Fingerprint ,Shelf life ,Aquatic Science ,Biochemistry ,microbiota ,Quality (business) ,TD201-500 ,Mineral water ,mineral water ,Water Science and Technology ,media_common ,Water supply for domestic and industrial purposes ,Waste management ,Microbiota ,Hydraulic engineering ,fingerprints ,Bottled water ,traceability ,Metagenomics ,NGS ,Environmental science ,Early warning system ,shelf life ,Water quality ,TC1-978 ,Risk assessment - Abstract
The microbiological monitoring of mineral bottled waters results is crucial for the prevention of outbreaks in consumers. European and International regulations establish the quality of water intended for human consumption in order to preserve human health from the negative effects deriving from water contamination. Advanced methods targeting the faster detection of potential pathogens in drinking water may consent to the creation of an early warning system, enhancing water quality management. This study aimed to suggest the implementation of standard water quality evaluations, based on the characterization of the microbial composition of mineral bottled water brands, contributing to the periodic control of the water’s microbiological stability along with the shelf life, and, consequently, the stability of the supplying sources. Bottled water microbiota analysis was combined with the qualitative and quantitative evaluation of microbial loads in time, and the monitoring was performed in two seasons and two different storage conditions for a total of sixty days. The employment of molecular microbiology techniques (NGS and Sanger sequencing), compared to standardized cultural methods and integrated with metagenomic analysis, combining chemical and physical indicators for each sample, allowing for the generation of specific fingerprints for mineral bottled waters, pointing at simplifying and improving the foreseen risk assessment strategies to ensure the adequate traceability, quality and safety management of drinking water.
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- 2021
32. Routine minimalist transcatheter aortic valve implantation with local anesthesia only
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Nazzareno Galiè, Nevio Taglieri, Miriam Compagnone, Francesco Saia, Cinzia Marrozzini, Chiara Marcelli, Matteo Chiarabelli, Andrea Castelli, Antonio Bruno, Anna Corsini, Tullio Palmerini, Gabriele Ghetti, Carolina Moretti, Fabio Negrello, Saia F., Palmerini T., Marcelli C., Chiarabelli M., Taglieri N., Ghetti G., Negrello F., Moretti C., Bruno A.G., Compagnone M., Corsini A., Castelli A., Marrozzini C., and Galie N.
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Male ,medicine.medical_specialty ,Time Factors ,Transcatheter aortic ,Sedation ,medicine.medical_treatment ,Conscious Sedation ,030204 cardiovascular system & hematology ,Anesthesia, General ,Risk Assessment ,Transcatheter Aortic Valve Replacement ,03 medical and health sciences ,0302 clinical medicine ,Postoperative Complications ,Valve replacement ,Risk Factors ,Anesthesiology ,medicine ,Humans ,Minimally Invasive Surgical Procedures ,minimalist ,Local anesthesia ,030212 general & internal medicine ,Stroke ,transcatheter aortic valve implantation ,Aged ,Aged, 80 and over ,business.industry ,transfemoral ,General Medicine ,Aortic Valve Stenosis ,aortic stenosi ,Length of Stay ,medicine.disease ,Treatment Outcome ,Cardiothoracic surgery ,Anesthesia ,Aortic Valve ,Cohort ,Feasibility Studies ,Female ,Patient Safety ,medicine.symptom ,local anesthesia ,Cardiology and Cardiovascular Medicine ,business ,Anesthesia, Local - Abstract
Aims Conscious sedation instead of general anesthesia has been increasingly adopted in many centers for transfemoral transcatheter aortic valve replacement (TAVR). Improvement of materials and operators' experience and reduction of periprocedural complications allowed procedural simplification and adoption of a minimalist approach. With this study, we sought to assess the feasibility and safety of transfemoral TAVR routinely performed under local anesthesia without on-site anesthesiology support. Methods The routine transfemoral TAVR protocol adopted at our center includes a minimalist approach, local anesthesia alone with fully awake patient, anesthesiologist available on call but not in the room, and direct transfer to the cardiology ward after the procedure. All consecutive patients undergoing transfemoral TAVR between January 2015 and July 2018 were included. We assessed the rates of actual local anesthesia-only procedures, conversion to conscious sedation or general anesthesia and 30-day clinical outcomes. Results Among 321 patients, 6 received general anesthesia upfront and 315 (98.1%) local anesthesia only. Mean age of the local anesthesia group was 83.2 ± 6.9 years, Society of Thoracic Surgery score 5.8 ± 4.8%. A balloon-expandable valve was used in 65.7%. Four patients (1.3%) shifted to conscious sedation because of pain or anxiety; 6 patients (1.9%) shifted to general anesthesia because of procedural complications. Hence, local anesthesia alone was possible in 305 patients (96.8% of the intended cohort, 95% of all transfemoral procedures). At 30 days, in the intended local anesthesia group, mortality was 1.6%, stroke 0.6%, major vascular complications 2.6%. Median hospital stay was 4 days (IQR 3-7). Conclusion Transfemoral TAVR can be safely performed with local anesthesia alone and without an on-site anesthesiologist in the vast majority of patients.
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- 2020
33. New transcatheter strategies for multiple pathologies: a paradigmatic clinical case
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Miriam, Compagnone, Antonio Giulio, Bruno, Francesco, Saia, Compagnone M., Bruno A.G., and Saia F.
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Aged, 80 and over ,Heart Failure ,Male ,Percutaneous mitral valve repair ,Dual Anti-Platelet Therapy ,Mitral Valve Insufficiency ,Drug-Eluting Stents ,Atrial fibrillation ,Angina Pectoris ,Percutaneous Coronary Intervention ,Percutaneous left atrial appendage occlusion ,cardiovascular system ,Humans ,Atrial Appendage ,cardiovascular diseases ,High bleeding risk ,Platelet Aggregation Inhibitors ,Mitral regurgitation - Abstract
Percutaneous transcatheter left atrial appendage occlusion and transcatheter mitral valve repair with the MitraClip system represent new therapeutic strategies for selected patients at high risk for both hemorrhagic and cardioembolic events or with symptomatic heart failure and moderate-to-severe mitral valve regurgitation, respectively. We report the case of an 84-year-old patient with severe degenerative mitral regurgitation hospitalized for a first episode of atrial fibrillation, angina pectoris and heart failure. The patient presented a clinical history of spontaneous cerebral bleeding, severe three-vessel coronary disease and multiple comorbidities that contraindicated a conventional surgical treatment. After an accurate clinical-instrumental evaluation, the local Heart Team indicated a combined procedure of percutaneous left atrial appendage closure and transcatheter mitral valve repair with the MitraClip system, followed by multivessel percutaneous coronary intervention (PCI) with drug-eluting stent implantation. Dual antiplatelet treatment was prescribed for 12 months after PCI.
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- 2020
34. COVID-eVax, an electroporated DNA vaccine candidate encoding the SARS-CoV-2 RBD, elicits protective responses in animal models
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Abraham Nyska, Alessia Muzi, Fabio Palombo, Luigi Aurisicchio, Mirela Kuka, Nicola Clementi, Concetta Castilletti, Valerio Chiarini, Erika Salvatori, Emanuele Marra, Alina Seidel, Francesca Colavita, Roberto Arriga, Valeria Fumagalli, Giulia Matusali, Laura Luberto, Lorena Donnici, Maria Lucrezia Pacello, Davide Marotta, Fabiana Fosca Ferrara, Eleonora Sala, Amy Rose Challis, Nicasio Mancini, Mariano Maffei, Eleonora Pinto, Gennaro Ciliberto, Emiliano Pavoni, Daniela Stoppoloni, Giuseppe Roscilli, Matteo Iannacone, Emanuela D’Acunto, Lucia Lione, Rüdiger Groß, Lukas Wettstein, Antonella Conforti, Federica Bucci, Elisa Bono, Jemma Paterson, Maria Rosaria Capobianchi, Gianfranco Caselli, Kathryn A. Ryan, Grazia Vitagliano, Jan Münch, Lucio C. Rovati, Matteo Conti, Giuseppe Ippolito, Elena Criscuolo, Chiara Perucchini, Micol Ravà, Manuela Cappelletti, Pietro Di Lucia, Leonardo Giustini, Raffaele De Francesco, Luca G. Guidotti, Mirco Compagnone, Conforti, A., Marra, E., Palombo, F., Roscilli, G., Rava, M., Fumagalli, V., Muzi, A., Maffei, M., Luberto, L., Lione, L., Salvatori, E., Compagnone, M., Pinto, E., Pavoni, E., Bucci, F., Vitagliano, G., Stoppoloni, D., Pacello, M. L., Cappelletti, M., Ferrara, F. F., D'Acunto, E., Chiarini, V., Arriga, R., Nyska, A., Di Lucia, P., Marotta, D., Bono, E., Giustini, L., Sala, E., Perucchini, C., Paterson, J., Ryan, K. A., Challis, A. -R., Matusali, G., Colavita, F., Caselli, G., Criscuolo, E., Clementi, N., Mancini, N., Gross, R., Seidel, A., Wettstein, L., Munch, J., Donnici, L., Conti, M., De Francesco, R., Kuka, M., Ciliberto, G., Castilletti, C., Capobianchi, M. R., Ippolito, G., Guidotti, L. G., Rovati, L., Iannacone, M., and Aurisicchio, L.
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Genetically modified mouse ,DNA vaccine ,COVID-19 Vaccines ,Mice, Transgenic ,Antibodies, Viral ,DNA vaccination ,Rats, Sprague-Dawley ,Mice ,Plasmid ,Protein Domains ,Complementary DNA ,Drug Discovery ,Genetics ,Vaccines, DNA ,Animals ,Humans ,Neutralizing antibody ,Molecular Biology ,Pharmacology ,Mice, Inbred BALB C ,biology ,SARS-CoV-2 ,Electroporation ,Immunogenicity ,Ferrets ,COVID-19 ,protection ,Virology ,Antibodies, Neutralizing ,animal models ,antiviral immunity ,Mice, Inbred C57BL ,Viral replication ,Models, Animal ,Spike Glycoprotein, Coronavirus ,biology.protein ,Molecular Medicine ,Female ,Immunization ,Original Article - Abstract
The COVID-19 pandemic caused by SARS-CoV-2 has made the development of safe and effective vaccines a critical priority. To date, four vaccines have been approved by European and American authorities for preventing COVID-19, but the development of additional vaccine platforms with improved supply and logistics profiles remains a pressing need. Here we report the preclinical evaluation of a novel COVID-19 vaccine candidate based on the electroporation of engineered, synthetic cDNA encoding a viral antigen in the skeletal muscle. We constructed a set of prototype DNA vaccines expressing various forms of the SARS-CoV-2 spike (S) protein and assessed their immunogenicity in animal models. Among them, COVID-eVax—a DNA plasmid encoding a secreted monomeric form of SARS-CoV-2 S protein receptor-binding domain (RBD)—induced the most potent anti-SARS-CoV-2 neutralizing antibody responses (including against the current most common variants of concern) and a robust T cell response. Upon challenge with SARS-CoV-2, immunized K18-hACE2 transgenic mice showed reduced weight loss, improved pulmonary function, and lower viral replication in the lungs and brain. COVID-eVax conferred significant protection to ferrets upon SARS-CoV-2 challenge. In summary, this study identifies COVID-eVax as an ideal COVID-19 vaccine candidate suitable for clinical development. Accordingly, a combined phase I-II trial has recently started., Graphical abstract, We report the development, characterization, and preclinical evaluation of COVID-eVax, a novel COVID-19 vaccine candidate with improved supply and logistics profiles. The technology is based on the electroporation of engineered, synthetic cDNA encoding a secreted monomeric form of the receptor-binding domain of the SARS-CoV-2 spike protein.
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35. Surgical Risk Scores Applied to Transcatheter Aortic Valve Implantation: Friends or Foes? Short-Term and Long-Term Outcomes From a Single-Center Registry
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Compagnone, Miriam, Moretti, Carolina, Marcelli, Chiara, Taglieri, Nevio, Ghetti, Gabriele, Corsini, Anna, Bruno, Matteo, Bruno, Antonio Giulio, Orzalkiewicz, Mateusz, Marrozzini, Cinzia, Reggiani, Maria-Letizia Bacchi, Tullio Palmerini, Saia, Francesco, Compagnone M., Moretti C., Marcelli C., Taglieri N., Ghetti G., Corsini A., Bruno M., Bruno A.G., Orzalkiewicz M., Marrozzini C., Reggiani M.-L.B., Palmerini T., and Saia F.
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Aortic stenosi ,Logistic euroscore ,Risk score ,Euroscore II ,Sts - Abstract
Background. Transcatheter aortic valve implantation (TAVI) is a valid alternative to surgical aortic valve replacement for the treatment of symptomatic aortic stenosis. The EuroScore (ES) II, logistic EuroScore (log ES), and the Society of Thoracic Surgeons (STS) score are the most applied scores for surgical risk stratification. However, their predictive value for patients undergoing TAVI is still unclear. Aim. To evaluate the performance of STS, log ES and ES II as predictors of short-term and long-term mortality in patients undergoing TAVI. Methods. Between February 2008 and October 2017, a total of 384 patients underwent transfemoral TAVI at our institution and constituted the study population. Patients were divided into three groups based on the class of risk (low, intermediate, and high) calculated by each score. In-hospital complications, 30-day outcomes, and 5-year outcomes were assessed. Results. In-hospital mortality rate was 2.6% (n = 10). All scores over-estimated the risk of 30-day mortality, especially for the highest risk classes. At the end of follow-up (5 years), STS risk stratification was able to stratify all-cause and cardiovascular (CV) mortality (P
36. Aortic balloon valvuloplasty outcome according to calcium distribution and valve geometry - The ABCD study.
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Dall'Ara G, Piciucchi S, Moretti C, Cavazza C, Compagnone M, Guerrieri G, Grotti S, Spartà D, Carletti R, Fabbri E, Giampalma E, Santarelli A, Ottani F, Balducelli M, Saia F, Tarantino FF, and Galvani M
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Background: There is little data on the outcome of balloon aortic valvuloplasty (BAV) in relation to valve dimensions and calcification patterns. The procedure is not standardized, particularly the choice of balloon size., Methods: This retrospective multicenter study focused on BAV efficacy and safety by analyzing the relationship between balloon size, annulus geometry (i.e., diameters, perimeter, and area), and calcification patterns (total burden and calcium distribution over each individual leaflet). From March 2018 to March 2023, all consecutive patients who underwent clinically indicated BAV and ECG-gated multidetector computed tomography of the aorta were included, except those with a bicuspid valve. Calcium score was calculated on contrast-enhanced images based on a luminal attenuation threshold of +100 HU., Results: One hundred and fifteen patients were included. Procedural success was 82.6 %. The balloon-to-annulus ratio (BAR) relative to diameter, perimeter, and area was higher in patients with successful BAV. Patients with unsuccessful BAV had a significantly higher aortic valve calcium burden. The complication rate was 4.3 % and there was no association with valve geometry or calcium burden. A trend towards a reduced complication rate was found as calcium asymmetry increased. BAR minimum annulus diameter was the best parameter in predicting procedural success, with a cut-off at 0.85., Conclusions: BAV efficacy is correlated directly with balloon size in relation to annulus dimension and inversely with total calcium burden. The minimum diameter of the valve may be adopted as a reference for balloon sizing., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)
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- 2024
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37. Predictability of varicocele repair success: preliminary results of a machine learning-based approach.
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Crafa A, Russo M, Cannarella R, Gül M, Compagnone M, Mongioì LM, Cannarella V, Condorelli RA, La Vignera S, and Calogero AE
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Varicocele is a prevalent condition in the infertile male population. However, to date, which patients may benefit most from varicocele repair is still a matter of debate. The purpose of this study was to evaluate whether certain preintervention sperm parameters are predictive of successful varicocele repair, defined as an improvement in total motile sperm count (TMSC). We performed a retrospective study on 111 patients with varicocele who had undergone varicocele repair, collected from the Department of Endocrinology, Metabolic Diseases and Nutrition, University of Catania (Catania, Italy), and the Unit of Urology at the Selcuk University School of Medicine (Konya, Türkiye). The predictive analysis was conducted through the use of the Brain Project, an innovative tool that allows a complete and totally unbiased search of mathematical expressions that relate the object of study to the various parameters available. Varicocele repair was considered successful when TMSC increased by at least 50% of the preintervention value. For patients with preintervention TMSC below 5 × 106, improvement was considered clinically relevant when the increase exceeded 50% and the absolute TMSC value was >5 × 106. From the preintervention TMSC alone, we found a model that predicts patients who appear to benefit little from varicocele repair with a sensitivity of 50.0% and a specificity of 81.8%. Varicocele grade and serum follicle-stimulating hormone (FSH) levels did not play a predictive role, but it should be noted that all patients enrolled in this study were selected with intermediate- or high-grade varicocele and normal FSH levels. In conclusion, preintervention TMSC is predictive of the success of varicocele repair in terms of TMSC improvement in patients with intermediate- or high-grade varicoceles and normal FSH levels., (Copyright © 2024 Copyright: ©The Author(s)(2024).)
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- 2024
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38. Case Report: Asymptomatic SARS-COV2 infection triggering recurrent Takotsubo syndrome.
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Dall'Ara G, Compagnone M, Carletti R, Piciucchi S, Gardini E, and Galvani M
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Takotsubo syndrome (TTS) is a rare disease mimicking acute coronary syndrome, often triggered by physical or emotional stress, and characterized by transient left ventricular dysfunction. Recurrences are described in about 5% of cases and may have different clinical and imaging patterns. In the present report, SARS-COV-2 infection, even in the absence of symptoms and overt emotional stress, seems correlated with recurrence of TTS, due to the absence of other recognized triggers. The hypothesis is that in predisposed patients, events like catecholamine-induced myocyte injury, direct viral damage, cytokine storm, immune-mediated damage, and procoagulant state, all possibly induced by the infection, may elicit endothelial dysfunction as substrate for TTS onset., Competing Interests: The authors declare the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (© 2024 Dall'Ara, Compagnone, Carletti, Piciucchi, Gardini and Galvani.)
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- 2024
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39. Dynamics of humoral and cellular response to three doses of anti-SARS-CoV-2 BNT162b2 vaccine in patients with hematological malignancies and older subjects.
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Laquintana V, Mottini C, Marchesi F, Marcozzi B, Terrenato I, Sperandio E, de Latouliere L, Carrieri F, Pimpinelli F, Pontone M, Pellini R, Campo F, Conti L, Accetta C, Mandoj C, Petrone F, Di Bella O, Vujovic B, Morrone A, Compagnone M, Principato E, Pinto E, Papa E, Falcucci P, La Malfa A, Pallocca M, De Marco F, Piaggio G, Ciliberto G, Mengarelli A, and di Martino S
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- Humans, COVID-19 Vaccines, BNT162 Vaccine, SARS-CoV-2, Antibodies, Neutralizing, Antibodies, Viral, COVID-19 prevention & control, Hematologic Neoplasms, Spike Glycoprotein, Coronavirus
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Background: Few data are available about the durability of the response, the induction of neutralizing antibodies, and the cellular response upon the third dose of the anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine in hemato-oncological patients., Objective: To investigate the antibody and cellular response to the BNT162b2 vaccine in patients with hematological malignancy., Methods: We measured SARS-CoV-2 anti-spike antibodies, anti- Omicron neutralizing antibodies, and T-cell responses 1 month after the third dose of vaccine in 93 fragile patients with hematological malignancy (FHM), 51 fragile not oncological subjects (FNO) aged 80-92, and 47 employees of the hospital (healthcare workers, (HW), aged 23-66 years. Blood samples were collected at day 0 (T0), 21 (T1), 35 (T2), 84 (T3), 168 (T4), 351 (T pre-3D), and 381 (T post-3D) after the first dose of vaccine. Serum IgG antibodies against S1/S2 antigens of SARS-CoV-2 spike protein were measured at every time point. Neutralizing antibodies were measured at T2, T3 (anti-Alpha), T4 (anti-Delta), and T post-3D (anti- Omicron ). T cell response was assessed at T post-3D., Results: An increase in anti-S1/S2 antigen antibodies compared to T0 was observed in the three groups at T post-3D. After the third vaccine dose, the median antibody level of FHM subjects was higher than after the second dose and above the putative protection threshold, although lower than in the other groups. The neutralizing activity of antibodies against the Omicron variant of the virus was tested at T2 and T post-3D. 42.3% of FHM, 80,0% of FNO, and 90,0% of HW had anti- Omicron neutralizing antibodies at T post-3D. To get more insight into the breadth of antibody responses, we analyzed neutralizing capacity against BA.4/BA.5, BF.7, BQ.1, XBB.1.5 since also for the Omicron variants, different mutations have been reported especially for the spike protein. The memory T-cell response was lower in FHM than in FNO and HW cohorts. Data on breakthrough infections and deaths suggested that the positivity threshold of the test is protective after the third dose of the vaccine in all cohorts., Conclusion: FHM have a relevant response to the BNT162b2 vaccine, with increasing antibody levels after the third dose coupled with, although low, a T-cell response. FHM need repeated vaccine doses to attain a protective immunological response., Competing Interests: Author MC is employed in Neomatrix, Rome, Italy. EPi is employed in Takis, Rome, Italy. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Laquintana, Mottini, Marchesi, Marcozzi, Terrenato, Sperandio, de Latouliere, Carrieri, Pimpinelli, Pontone, Pellini, Campo, Conti, Accetta, Mandoj, Petrone, Di Bella, Vujovic, Morrone, Compagnone, Principato, Pinto, Papa, Falcucci, La Malfa, Pallocca, De Marco, Piaggio, Ciliberto, Mengarelli and di Martino.)
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- 2024
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40. Isolation and Characterization of Neutralizing Monoclonal Antibodies from a Large Panel of Murine Antibodies against RBD of the SARS-CoV-2 Spike Protein.
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D'Acunto E, Muzi A, Marchese S, Donnici L, Chiarini V, Bucci F, Pavoni E, Ferrara FF, Cappelletti M, Arriga R, Serrao SM, Peluzzi V, Principato E, Compagnone M, Pinto E, Luberto L, Stoppoloni D, Lahm A, Groß R, Seidel A, Wettstein L, Münch J, Goodhead A, Parisot J, De Francesco R, Ciliberto G, Marra E, Aurisicchio L, and Roscilli G
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The COVID-19 pandemic, once a global crisis, is now largely under control, a testament to the extraordinary global efforts involving vaccination and public health measures. However, the relentless evolution of SARS-CoV-2, leading to the emergence of new variants, continues to underscore the importance of remaining vigilant and adaptable. Monoclonal antibodies (mAbs) have stood out as a powerful and immediate therapeutic response to COVID-19. Despite the success of mAbs, the evolution of SARS-CoV-2 continues to pose challenges and the available antibodies are no longer effective. New variants require the ongoing development of effective antibodies. In the present study, we describe the generation and characterization of neutralizing mAbs against the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein by combining plasmid DNA and recombinant protein vaccination. By integrating genetic immunization for rapid antibody production and the potent immune stimulation enabled by protein vaccination, we produced a rich pool of antibodies, each with unique binding and neutralizing specificities, tested with the ELISA, BLI and FACS assays and the pseudovirus assay, respectively. Here, we present a panel of mAbs effective against the SARS-CoV-2 variants up to Omicron BA.1 and BA.5, with the flexibility to target emerging variants. This approach ensures the preparedness principle is in place to address SARS-CoV-2 actual and future infections.
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- 2024
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41. Prolactin Inhibition to Treat Postpartum Arrhythmic Storm.
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Compagnone M, Marinelli A, Dall'Ara G, Ziacchi M, Grotti S, Ciurlanti L, Tarantino FF, Potena L, Biffi M, and Galvani M
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Postpartum electrical storm due to torsade de pointes is a rare but life-threatening condition. The uniqueness of this case lies in the use of cabergoline to suppress postpartum ventricular arrhythmias in absence of heart disease. Timely multidisciplinary management is crucial to achieve final diagnosis, deliver proper treatment and improve prognosis., Competing Interests: This research was funded by Fondazione Cardiologica M.Z. Sacco, Forlì, Italy. The authors have reported that they have no relationships relevant to the contents of this paper to disclose., (© 2024 The Authors.)
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- 2024
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42. Transcatheter Aortic Valve Replacement Without On-Site Cardiac Surgery: Ready for Prime Time?
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Compagnone M, Dall'Ara G, Grotti S, Santarelli A, Balducelli M, Savini C, Tarantino FF, and Galvani M
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- Humans, Treatment Outcome, Aortic Valve diagnostic imaging, Aortic Valve surgery, Risk Factors, Transcatheter Aortic Valve Replacement adverse effects, Aortic Valve Stenosis diagnostic imaging, Aortic Valve Stenosis surgery, Heart Valve Prosthesis
- Abstract
Competing Interests: Funding Support and Author Disclosures The authors have reported that they have no relationships relevant to the contents of this paper to disclose.
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- 2023
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43. Sustainable Recovery of Platinum Group Metals from Spent Automotive Three-Way Catalysts through a Biogenic Thiosulfate-Copper-Ammonia System.
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Compagnone M, González-Cortés JJ, Pilar Yeste M, Cantero D, and Ramírez M
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This study explores an eco-friendly method for recovering platinum group metals from a synthetic automotive three-way catalyst (TWC). Bioleaching of palladium (Pd) using the thiosulfate-copper-ammonia leaching processes, with biogenic thiosulfate sourced from a bioreactor used for biogas biodesulfurization, is proposed as a sustainable alternative to conventional methods. Biogenic thiosulfate production was optimized in a gas-lift bioreactor by studying the pH (8-10) and operation modes (batch and continuous) under anoxic and microaerobic conditions for 35 d. The maximum concentration of 4.9 g S
2 O3 2- L-1 of biogenic thiosulfate was reached under optimal conditions (batch mode, pH = 10, and airflow rate 0.033 vvm). To optimize Pd bioleaching from a ground TWC, screening through a Plackett-Burman design determined that oxygen and temperature significantly affected the leaching yield negatively and positively, respectively. Based on these results, an optimization through an experimental design was performed, indicating the optimal conditions to be Na2 S2 1.2 M, CuSO3 0.03 M, (NH4 0.03 M, (NH4 )2 1.5 M, Na4 1.5 M, Na2 0.2 M, pH 8, and 60 °C. A remarkable 96.2 and 93.2% of the total Pd was successfully extracted from the solid at 5% pulp density using both commercially available and biogenic thiosulfate, highlighting the method's versatility for Pd bioleaching from both thiosulfate sources.3 0.2 M, pH 8, and 60 °C. A remarkable 96.2 and 93.2% of the total Pd was successfully extracted from the solid at 5% pulp density using both commercially available and biogenic thiosulfate, highlighting the method's versatility for Pd bioleaching from both thiosulfate sources.- Published
- 2023
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44. A Glimpse Beyond Typical Dyspnea.
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Compagnone M, Tarantino FF, Grotti S, Pestelli G, Galvani M, and Dall'Ara G
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- 2023
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45. Bioleaching of the α-alumina layer of spent three-way catalysts as a pretreatment for the recovery of platinum group metals.
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Compagnone M, González-Cortés JJ, Yeste MDP, Cantero D, and Ramírez M
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- Acidithiobacillus thiooxidans, Catalysis, Sulfur, Platinum, Acidithiobacillus
- Abstract
Acid bioleaching of Al by Acidithiobacillus thiooxidans has been explored as an environmentally friendly pretreatment to facilitate the extraction of platinum group metals from spent three-way catalysts (TWC). Biogenic sulfur obtained from desulfurization bioreactors improved the production of acid by A. thiooxidans compared to commercially available elemental sulfur. The lixiviation abilities of bacteria-free biogenic acid and biogenic acid with exponential or stationary phase bacteria were compared against a control batch produced by commercial H
2 SO4 . The maximum Al leaching percentage (54.5%) was achieved using biogenic acids with stationary-phase bacteria at a TWC pulp density of 5% w/v whereas bacteria-free biogenic acid (23.4%), biogenic acid with exponential phase bacteria (21.7%) and commercial H2 SO4 (24.7%) showed lower leaching abilities. The effect of different pulp densities of ground TWC (5, 30, and 60% w/v) on Al leaching and bacterial growth was determined. While greater Al leaching yields were obtained at lower TWC pulp density solutions (54.5% at 5% w/v and 2.5% at 60% w/v), higher pulp densities enhanced microbial growth (2.3 × 109 cells/mL at 5% w/v and 9.5 × 1010 cells/mL at 60% w/v). The dissolution of the metal from the solid into the liquid phase triggered the production of biological polymeric substances that were able to absorb traces of both Al (up to 24.80% at 5% w/v) and Pt (up to 0.40% at 60% w/v)., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)- Published
- 2023
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46. 2-year outcomes of MitraClip as a bridge to heart transplantation: The international MitraBridge registry.
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Munafò AR, Scotti A, Estévez-Loureiro R, Adamo M, Hernàndez AP, Peregrina EF, Gutierrez L, Taramasso M, Fam NP, Ho EC, Asgar A, Vitrella G, Raineri C, Chizzola G, Pezzola E, Le Ruz R, Montalto C, Oreglia JA, Fraccaro C, Giannini C, Fiorelli F, Rubbio AP, Ooms JF, Compagnone M, Marcelli C, Maffeo D, Bettari L, Fürholz M, Arzamendi D, Guerin P, Tamburino C, Petronio AS, Grasso C, Agricola E, Van Mieghem NM, Tarantini G, Praz F, Pascual I, Potena L, Colombo A, Maisano F, Metra M, Margonato A, Crimi G, Saia F, and Godino C
- Subjects
- Humans, Middle Aged, Stroke Volume, Ventricular Function, Left, Time Factors, Treatment Outcome, Registries, Heart Transplantation adverse effects, Mitral Valve Insufficiency diagnostic imaging, Mitral Valve Insufficiency surgery, Heart Failure diagnosis, Heart Failure surgery, Heart Valve Prosthesis Implantation methods
- Abstract
Background: In the first report from the MitraBridge registry, MitraClip as a bridge to heart transplantation (HTx) proved to be at 1-year an effective treatment strategy for 119 patients with advanced heart failure (HF) who were potential candidates for HTx. We aimed to determine if benefits of MitraClip procedure as a bridge-to-transplant persist up to 2-years., Methods: By the end of the enrollment period, a total of 153 advanced HF patients (median age 59 years, left ventricular ejection fraction 26.9 ± 7.7%) with significant secondary mitral regurgitation, who were potential candidates for HTx and were treated with MitraClip as a bridge-to-transplant strategy, were included in the MitraBridge registry. The primary endpoint was the 2-year composite adverse events rate of all-cause death, first hospitalization for HF, urgent HTx or LVAD implantation., Results: Procedural success was achieved in 89.5% of cases. Thirty-day mortality was 0%. At 2-year, Kaplan-Meier estimates of freedom from primary endpoint was 47%. Through 24 months, the annualized rate of HF rehospitalization per patient-year was 44%. After an overall median follow-up time of 26 (9-52) months, elective HTx was successfully performed in 30 cases (21%), 19 patients (13.5%) maintained or obtained the eligibility for transplant, and 32 patients (22.5%) no longer had an indication for HTx because of significant clinical improvement., Conclusions: After 2-years of follow-up, the use of MitraClip as a bridge-to-transplant was confirmed as an effective strategy, allowing elective HTx or eligibility for transplant in one third of patients, and no more need for transplantation in 22.5% of cases., Competing Interests: Declaration of Competing Interest A.S. has served as a consultant and received consulting fees from NeoChord Inc.; R.E.L. received speaker fees from Abbott, Boston and Edwards; M.A. received speaker fees from Abbott Vascular and Medtronic; P.G. has been a proctor for Abbot; N.M.V.M. received research grant support from Abbott Vascular, Biotronik, Medtronik, Boston Scientific, Edwards Lifesciences, Daiichi Sankyo, Abiomed en PulseCath BV; F.S. received consultancy and lecture's fees from Abbott and Edwards., (Copyright © 2023 Elsevier B.V. All rights reserved.)
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- 2023
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47. [Right heart catheterization in Italian catheterization laboratories: results of the SICI-GISE national survey promoted by the GISE Young Committee].
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Compagnone M, Demola P, Serino F, Masiero G, Giuliani L, Rossi S, Polimeni A, Attisano T, Contarini M, Castiglioni B, De Marco F, Fineschi M, Menozzi A, Musto C, Saia F, Tarantini G, and Esposito G
- Subjects
- Humans, Laboratories, Cardiac Catheterization methods, Surveys and Questionnaires, Italy, Anticoagulants, Hypertension, Pulmonary
- Abstract
Background: Over the past decades, the improvements in the diagnostic power and availability of non-invasive cardiac imaging techniques have led to a decline of right heart catheterization (RHC) performance. However, RHC remains the gold standard for diagnosing pulmonary hypertension and an essential tool for the evaluation of patient candidacy to heart transplantation., Methods: This survey was carried out jointly by the Young Committee of GISE, with the support of the SICI-GISE Society, and the ICOT group, with the aim of evaluating how the interventional cardiology community perform RHC. A web-based questionnaire based on 20 questions was distributed to SICI-GISE members., Results: The survey was distributed to 1550 physicians with 174 (11%) responses. Most centers perform few procedures per year (<10 RHC/year) and a dedicated cardiologist is usually lacking. Patients were frequently admitted as ordinary hospitalization regimen and the most frequent indication for RHC was the hemodynamic assessment of pulmonary hypertension, followed by diagnostics of valvular diseases and advanced heart failure/heart transplantation. Indeed, the majority of participants (86%) are involved in transcatheter procedures for structural heart disease. The average time taken to perform the RHC was approximately 30-60 min. The femoral access (60%) was the most frequently used, usually by an echo-guided approach. Two-thirds of participants discontinued oral anticoagulant therapy before RHC. Only 27% of centers assess wedge position from an integrated analysis. Furthermore, the edge pressure is detected in the end-diastolic cardiac phase in half cases and in the end-expiratory phase in only 31%. The most commonly used method for cardiac output calculation was the indirect Fick method (58%)., Conclusions: Guidance on the best practice for performing RHC is currently lacking. A more precise standardization of this demanding procedure is warranted.
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- 2023
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48. A linear SARS-CoV-2 DNA vaccine candidate reduces virus shedding in ferrets.
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Martins M, do Nascimento GM, Conforti A, Noll JCG, Impellizeri JA, Sanchez E, Wagner B, Lione L, Salvatori E, Pinto E, Compagnone M, Viscount B, Hayward J, Shorrock C, Aurisicchio L, and Diel DG
- Subjects
- Humans, Animals, COVID-19 Vaccines, SARS-CoV-2, Ferrets, Virus Shedding, Antibodies, Viral, Antibodies, Neutralizing, DNA, Spike Glycoprotein, Coronavirus genetics, Immunogenicity, Vaccine, COVID-19 prevention & control, Vaccines, DNA genetics, Viral Vaccines
- Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), has caused more than 760 million cases and over 6.8 million deaths as of March 2023. Vaccination has been the main strategy used to contain the spread of the virus and to prevent hospitalizations and deaths. Currently, two mRNA-based vaccines and one adenovirus-vectored vaccine have been approved and are available for use in the U.S. population. The versatility, low cost, and rapid production of DNA vaccines provide important advantages over other platforms. Additionally, DNA vaccines efficiently induce both B- and T-cell responses by expressing the antigen within transfected host cells, and the antigen, after being processed into peptides, can associate with MHC class I or II of antigen-presenting cells (APCs) to stimulate different T cell responses. However, the efficiency of DNA vaccination needs to be improved for use in humans. Importantly, in vivo DNA delivery combined with electroporation (EP) has been used successfully in the field of veterinary oncology, resulting in high rates of response after electrochemotherapy. Here, we evaluate the safety, immunogenicity, and protective efficacy of a novel linear SARS-CoV-2 DNA vaccine candidate delivered by intramuscular injection followed by electroporation (Vet-ePorator™) in ferrets. The linear SARS-CoV-2 DNA vaccine candidate did not cause unexpected side effects. Additionally, the vaccine elicited neutralizing antibodies and T cell responses on day 42 post-immunization using a low dose of the linear DNA construct in a prime-boost regimen. Most importantly, vaccination significantly reduced shedding of infectious SARS-CoV-2 through oral and nasal secretions in a ferret model., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
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- 2023
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49. Immunogenicity of COVID-eVax Delivered by Electroporation Is Moderately Impacted by Temperature and Molecular Isoforms.
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D'Alessio F, Lione L, Salvatori E, Bucci F, Muzi A, Roscilli G, Compagnone M, Pinto E, Battistuzzi G, Conforti A, Aurisicchio L, and Palombo F
- Abstract
DNA integrity is a key issue in gene therapy and genetic vaccine approaches based on plasmid DNA. In contrast to messenger RNA that requires a controlled cold chain for efficacy, DNA molecules are considered to be more stable. In this study, we challenged this concept by characterizing the immunological response induced by a plasmid DNA vaccine delivered using electroporation. As a model, we used COVID-eVax, a plasmid DNA-based vaccine that targets the receptor binding domain (RBD) of the SARS-CoV-2 spike protein. Increased nicked DNA was produced by using either an accelerated stability protocol or a lyophilization protocol. Surprisingly, the immune response induced in vivo was only minimally affected by the percentage of open circular DNA. This result suggests that plasmid DNA vaccines, such as COVID-eVax that have recently completed a phase I clinical trial, retain their efficacy upon storage at higher temperatures, and this feature may facilitate their use in low-/middle-income countries.
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- 2023
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50. A linear DNA encoding the SARS-CoV-2 receptor binding domain elicits potent immune response and neutralizing antibodies in domestic cats.
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Conforti A, Sanchez E, Salvatori E, Lione L, Compagnone M, Pinto E, Palombo F, D'Acunto E, Muzi A, Roscilli G, Sun Y, Viscount B, Hayward J, Shorrock C, Diel DG, Impellizeri JA, and Aurisicchio L
- Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiologic agent of the COVID-19 pandemic, has been shown to infect a wide range of animal species, especially mammals, and besides human-to-human transmission, human-to-animal transmission has also been observed in some wild animals and pets, especially in cats. It has been demonstrated that cats are permissive to COVID-19 and are susceptible to airborne infections. Given the high transmissibility potential of SARS-CoV-2 to different host species and the close contact between humans and animals, it is crucial to find mechanisms to prevent the transmission chain and reduce the risk of spillover to susceptible species. Here, we show results from a clinical trial conducted in domestic cats to assess safety and immunogenicity of a linear DNA (linDNA) vaccine encoding the receptor-binding domain (RBD) from SARS-CoV-2 (Lin-COVID-eVax). Lin-COVID-eVax proved to be safe, with no significant adverse events, and was able to elicit both RBD-specific antibodies and T cells. Also, the linDNA vaccine induced neutralizing antibody titers against ancestral SARS-CoV-2 virus and its variants. These findings demonstrate the safety and immunogenicity of a genetic vaccine against COVID-19 administered to cats and strongly support the development of vaccines for preventing viral spread in susceptible species, especially those in close contact with humans., Competing Interests: Evvivax, Takis, and NeoMatrix are currently developing proprietary nucleic-acid vaccines based on DNA-EGT. Applied DNA and LineaRx are commercializing LinearDNA, its proprietary, large-scale PCR-based manufacturing platform that allows for the large-scale production of specific DNA sequences for biotherapeutic applications. The company’s common stock is listed on NASDAQ under ticker symbol “APDN,” and its publicly traded warrants are listed on OTC under ticker symbol “APPDW.”, (© 2023 The Authors.)
- Published
- 2023
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