Search

Your search keyword '"Cominato, M. L."' showing total 2 results
Start Over Author "Cominato, M. L."
2 results on '"Cominato, M. L."'

1. Global gene expression profiling of oral cavity cancers suggests molecular heterogeneity within anatomic subsites

Author

Severino, Patricia, Alvares, Adriana M., Michaluart, Pedro, Okamoto, Oswaldo K., Nunes, Fabio D., Moreira-Filho, Carlos A., Tajara, Eloiza H., Cury, P. M., Frizzera, A. P.Z., de Carvalho, M. B., Silva, A. M.A., Amar, A., Barbieri, R. B., Bastos, A. U., Carvalho-Neto, P. B., Casemiro, A. F., Chedid, H., Chiappini, P. B.O., Correia, L. A., Costa, A. C.W., Curioni, O. A., Franzi, S. A., Gazito, D., Gutierres, A. P., Lehn, C. N., Martins, A. E., Mercante, A. M.C., Porsani, A. F., Rapoport, A., Rossi, L., Santos, M., Souza, T. B., Takamori, J. T., Dias-Neto, E., Ojopi, E. P.B., Dias, T. H.G., Figueiredo, D. L.A., Mamede, R. C.M., Fukuyama, E. E., Góis-Filho, J. F., Cerione, M., Cicco, R., Settani, F., Valentim, P. J., Yamagushi, F., Cominato, M. L., Mendes, G. S., Paiva, R., Silva, M. J., Leopoldino, A. M., Silva, F. A.M., Moyses, R. A., Arap, S. S., Araújo, N. S.S., Araújo-Filho, V., Brandão, L. G., Cernea, C. R., Durazzo, M., Ferraz, A. R., Gallo, J., Guimarães, P. E.M., Magalhães, R. P., Montenegro, F. L.M., Silva-Filho, G. B., Smith, R. B., Stabenow, E., Tavares, M. R., Turcano, R., Volpi, E. M., Ramos, O., Silva, C., Moreira-Filho, C. A., Nóbrega, F. G. [UNESP], Nóbrega, M. P. [UNESP], Canto, A. L. [UNESP], Macarenco, R. [UNESP], Meneses, C. [UNESP], Correa, P. M.S. [UNESP], Bogossian, A. P. [UNESP], Nunes, F. D., Souza, S. C.O.M., Rodini, C. O., Xavier, F. C.A., Okamoto, O. K., Serafini, L. N., Severino, P., Silva, W. A., Brandão, R. M., Kaneto, C. M., Pinheiro, D. G., Santos, A. R.D., Silva, I. T., Tarlá, M. V.C., Silveira, N. J.F., Tajara, E. H., Rodrigues-Lisoni, F. C., Rodrigues, R. V., Polachini, G. M., Vidotto, A., Cunha, B. R., Carmona-Raphe, J., Wünsch-Filho, V., Costa, A., Figueiredo, R. O., Fortes, C. S., Inamine, R., López, R. V.M., Rodrigues, A. N., Zago, M. A., Instituto Israelita de Ensino e Pesquisa Albert Einstein, Hospital Heliópolis, Universidade de São Paulo (USP), Universidade Federal de São Paulo (UNIFESP), Faculdade de Medicina de São José do Rio Preto, Faculdade de Medicina, Instituto do Câncer Arnaldo Vieira de Carvalho, Universidade Estadual Paulista (UNESP), Instituto de Ensino e Pesquisa Albert Einstein, and UNIVAP

Subjects
Medicine(all), Microarray, Cytoskeleton organization, business.industry, Biochemistry, Genetics and Molecular Biology(all), lcsh:R, Short Report, lcsh:Medicine, General Medicine, Disease, Cell cycle, Bioinformatics, General Biochemistry, Genetics and Molecular Biology, Gene expression profiling, stomatognathic diseases, lcsh:Biology (General), Gene expression, Gene chip analysis, Medicine, lcsh:Science (General), business, lcsh:QH301-705.5, Gene, lcsh:Q1-390
Abstract

Made available in DSpace on 2022-04-29T08:44:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-01-01 Background: Oral squamous cell carcinoma (OSCC) is a frequent neoplasm, which is usually aggressive and has unpredictable biological behavior and unfavorable prognosis. The comprehension of the molecular basis of this variability should lead to the development of targeted therapies as well as to improvements in specificity and sensitivity of diagnosis. Results: Samples of primary OSCCs and their corresponding surgical margins were obtained from male patients during surgery and their gene expression profiles were screened using whole-genome microarray technology. Hierarchical clustering and Principal Components Analysis were used for data visualization and One-way Analysis of Variance was used to identify differentially expressed genes. Samples clustered mostly according to disease subsite, suggesting molecular heterogeneity within tumor stages. In order to corroborate our results, two publicly available datasets of microarray experiments were assessed. We found significant molecular differences between OSCC anatomic subsites concerning groups of genes presently or potentially important for drug development, including mRNA processing, cytoskeleton organization and biogenesis, metabolic process, cell cycle and apoptosis. Conclusion: Our results corroborate literature data on molecular heterogeneity of OSCCs. Differences between disease subsites and among samples belonging to the same TNM class highlight the importance of gene expression-based classification and challenge the development of targeted therapies. Centro de Pesquisa Experimental Instituto Israelita de Ensino e Pesquisa Albert Einstein Laboratório de Biologia Molecular Hospital Heliópolis Departamento de Cirurgia de Cabeça e Pescoço Hospital das Clínicas Faculdade de Medicina Universidade de São Paulo Departamento de Neurologia e Neurocirurgia Universidade Federal de São Paulo Departamento de Estomatologia Faculdade de Odontologia Universidade de São Paulo Departamento de Pediatria Faculdade de Medicina Universidade de São Paulo Departamento de Biologia Molecular Faculdade de Medicina de São José do Rio Preto Departamento de Genética e Biologia Evolutiva Instituto de Biociências Universidade de São Paulo Departamento de Patologia Faculdade de Medicina Hospital Heliópolis Departamento e Instituto de Psiquiatria Faculdade de Medicina USP Serviço de Cirurgia de Cabeça e Pescoço Faculdade de Medicina de Ribeirão Preto USP Serviço de Cirurgia de Cabeça e Pescoço Instituto do Câncer Arnaldo Vieira de Carvalho Departamento de Análises Clínicas Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto USP Departamento de Cirurgia de Cabeça e Pescoço Faculdade de Medicina USP Departamento de Pediatria Faculdade de Medicina USP Departamento de Biociências e Diagnóstico Bucal Faculdade de Odontologia UNESP Departamento de Estomatologia Faculdade de Odontologia USP Departamento de Neurologia e Neurocirurgia UNIFESP Departamento de Patologia Faculdade de Medicina de Ribeirão Preto USP Instituto de Ensino e Pesquisa Albert Einstein Departamento de Genética Faculdade de Medicina de Ribeirão Preto USP Ciências da Computação UNIVAP Departamento de Biologia Molecular Faculdade de Medicina Departamento de Epidemiologia Faculdade de Saúde Pública USP Departamento de Clínica Médica Faculdade de Medicina de Ribeirão Preto USP Departamento de Biociências e Diagnóstico Bucal Faculdade de Odontologia UNESP

Published
2008

2. Genomics and proteomics approaches to the study of cancer-stroma interactions.

Author

Rodrigues-Lisoni FC, Peitl P Jr, Vidotto A, Polachini GM, Maniglia JV, Carmona-Raphe J, Cunha BR, Henrique T, Souza CF, Teixeira RA, Fukuyama EE, Michaluart P Jr, de Carvalho MB, Oliani SM, Tajara EH, Cury PM, de Carvalho MB, Dias-Neto E, Figueiredo DL, Fukuyama EE, Góis-Filho JF, Leopoldino AM, Mamede RC, Michaluart-Junior P, Moyses RA, Nóbrega FG, Nóbrega MP, Nunes FD, Ojopi EF, Serafini LN, Severino P, Silva AM, Silva WA Jr, Silveira NJ, Souza SC, Tajara EH, Wünsch-Filho V, Amar A, Bandeira CM, Braconi MA, Brandão LG, Brandão RM, Canto AL, Cerione M, Cicco R, Chagas MJ, Chedid H, Costa A, Cunha BR, Curioni OA, Fortes CS, Franzi SA, Frizzera AP, Gazito D, Guimarães PE, Kaneto CM, López RV, Macarenco R, Magalhães MR, Meneses C, Mercante AM, Pinheiro DG, Polachini GM, Rapoport A, Rodini CO, Rodrigues-Lisoni FC, Rodrigues RV, Rossi L, Santos AR, Santos M, Settani F, Silva FA, Silva IT, Souza TB, Stabenow E, Takamori JT, Valentim PJ, Vidotto A, Xavier FC, Yamagushi F, Cominato ML, Correa PM, Mendes GS, Paiva R, Ramos O, Silva C, Silva MJ, and Tarlá MV

Subjects
Annexin A5 metabolism, Apoptosis, Cell Proliferation, Down-Regulation, Electrophoresis, Gel, Two-Dimensional, Fibroblasts metabolism, Genomics, Hep G2 Cells, Humans, Keratins metabolism, Mouth Neoplasms genetics, Nucleic Acid Hybridization, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Stromal Cells metabolism, Vimentin metabolism, Gene Expression Regulation, Neoplastic, Mouth Neoplasms metabolism, Proteome metabolism
Abstract

Background: The development and progression of cancer depend on its genetic characteristics as well as on the interactions with its microenvironment. Understanding these interactions may contribute to diagnostic and prognostic evaluations and to the development of new cancer therapies. Aiming to investigate potential mechanisms by which the tumor microenvironment might contribute to a cancer phenotype, we evaluated soluble paracrine factors produced by stromal and neoplastic cells which may influence proliferation and gene and protein expression., Methods: The study was carried out on the epithelial cancer cell line (Hep-2) and fibroblasts isolated from a primary oral cancer. We combined a conditioned-medium technique with subtraction hybridization approach, quantitative PCR and proteomics, in order to evaluate gene and protein expression influenced by soluble paracrine factors produced by stromal and neoplastic cells., Results: We observed that conditioned medium from fibroblast cultures (FCM) inhibited proliferation and induced apoptosis in Hep-2 cells. In neoplastic cells, 41 genes and 5 proteins exhibited changes in expression levels in response to FCM and, in fibroblasts, 17 genes and 2 proteins showed down-regulation in response to conditioned medium from Hep-2 cells (HCM). Nine genes were selected and the expression results of 6 down-regulated genes (ARID4A, CALR, GNB2L1, RNF10, SQSTM1, USP9X) were validated by real time PCR., Conclusions: A significant and common denominator in the results was the potential induction of signaling changes associated with immune or inflammatory response in the absence of a specific protein.

Published
2010
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results