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5. Babesiosis in the immunocompromised population: Results from a multicentric cohort study conducted in Italy

Author

Barbiero, Anna, Gabrielli, Simona, Dani, Lapo, Spinicci, Michele, Lagi, Filippo, Basile, Gregorio, Nacci, Francesca, Mantella, Antonia, Kiros, Seble Tekle, Pieri, Angela, Delama, Andrea, Piubelli, Chiara, Scarso, Salvatore, Angheben, Andrea, Feasi, Marcello, Granozzi, Bianca, Comai, Giorgia, Varani, Stefania, Zammarchi, Lorenzo, and Bartoloni, Alessandro

Published
2024
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6. Recurrence of membranous nephropathy after kidney transplantation: A multicenter retrospective cohort study

Author

Hullekes, Frank, Uffing, Audrey, Verhoeff, Rucháma, Seeger, Harald, von Moos, Seraina, Mansur, Juliana, Mastroianni-Kirsztajn, Gianna, Silva, Helio Tedesco, Buxeda, Anna, Pérez-Sáez, María José, Arias-Cabrales, Carlos, Collins, A. Bernard, Swett, Christie, Morená, Leela, Loucaidou, Marina, Kousios, Andreas, Malvezzi, Paolo, Bugnazet, Mathilde, Russo, Luis Sanchez, Muhsin, Saif A., Agrawal, Nikhil, Nissaisorakarn, Pitchaphon, Patel, Het, Al Jurdi, Ayman, Akalin, Enver, Neto, Elias David, Agena, Fabiana, Ventura, Carlucci, Manfro, Roberto C., Bauer, Andrea Carla, Mazzali, Marilda, de Sousa, Marcos Vinicius, La Manna, Gaetano, Bini, Claudia, Comai, Giorgia, Reindl-Schwaighofer, Roman, Berger, Stefan, Cravedi, Paolo, and Riella, Leonardo V.

Published
2024
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8. Immunosuppressive therapy and oral anticoagulation in kidney transplant recipients: Direct oral anticoagulants versus vitamin-k antagonists

Author

Santoro, Francesco, Casanova, Annalisa, Simone, Simona, Alfieri, Carlo, Falcone, Adele, Dello Strologo, Andrea, Grandinetti, Valeria, Busutti, Marco, Comai, Giorgia, Marvulli, Tommaso Maria, Zippo, Maria Grazia, Castellano, Giuseppe, La Manna, Gaetano, Gesualdo, Loreto, Giuseppe, Grandaliano, and Pesce, Francesco

Published
2024
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16. Renal Benefits of CO2 as a Contrast Media for EVAR Procedures: New Perspectives on 1 Year Outcomes.

Author

Busutti, Marco, Sensoni, Alice, Vacirca, Andrea, Abenavoli, Chiara, Donadei, Chiara, Croci Chiocchini, Anna Laura, Righini, Matteo, Comai, Giorgia, Pini, Alessia, Faggioli, Gianluca, Gallitto, Enrico, La Manna, Gaetano, and Gargiulo, Mauro

Abstract

Background and Objectives: Endovascular aneurism repair (EVAR) is a minimally invasive alternative to open surgery for the treatment of abdominal aortic aneurysm. Iodine contrast medium (ICM) is considered the gold standard, at the high price of related nephrotoxicity and allergic reactions. Carbon dioxide (CO2) has been suggested as an alternative non-nephrotoxic contrast media agent. We aimed to evaluate the safety and the renal impact of the administration of CO2, compared with ICM in EVAR procedures. Design, Setting, Participants, and Measurements: We retrospectively reviewed data of patients who underwent EVAR at the Vascular Surgery Department of the Sant'Orsola Hospital in Bologna. Estimated glomerular filtration rate (eGFR) was evaluated before intervention, immediately after and at 12 months. Results: In total, 22 patients received CO2 and low-dose ICM (CO2 Group) and 22 received standard ICM (Control Group), matched for clinical characteristics and renal function at the time of procedure. Pre and post-operative renal function values (eGFR) were compared between the two groups: in the immediate post-operative the group treated with CO2 and low-dose ICM globally showed a slight improvement in renal function (mean eGFR +5.10%±3.2), meanwhile the group treated with standard dose of ICM presented a significant worsening of renal function compared with pre-procedure values (mean eGFR −9.65%±4). Incidence of post-contrast acute kidney injury (PC-AKI) was 9% in the CO2 group vs 27% in the Control group. At 12 months, the renal impairment was significantly greater in the ICM group than in the CO2 group (mean eGFR decrease −19.2%±11.1 and −7.40%±3.5, respectively). Conclusions: Administration of either CO2 alone or along with low-dose ICM showed to be safer than full-dose ICM alone, lowering the incidence of PC-AKI in patients undergoing EVAR. Unexpectedly, our study revealed also a significant worsening of renal function in patients treated with standard dose of ICM in 1-year follow-up, introducing the concept that acute renal damage caused by ICM could elicit a chronic injury process that affect long-term renal outcomes. Clinical Impact: Evaluating the safety and the renal impact of the administration of CO2, compared to Iodinate Contrast Medium, in EVAR procedures represents a first step in order to further tayloring medical procedures on patients characteristics. Our findings can guide the clinicians and surgeons in the procedures choice, not considering only the immediate effect of ICM on renal function but also the potential long-term effects. [ABSTRACT FROM AUTHOR]

Published
2024
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17. Results from the IRoc-GN international registry of patients with COVID-19 and glomerular disease suggest close monitoring

Author

Waldman, Meryl, Soler, Maria Jose, García-Carro, Clara, Lightstone, Liz, Turner-Stokes, Tabitha, Griffith, Megan, Torras, Joan, Valenzuela, Laura Martinez, Bestard, Oriol, Geddes, Colin, Flossmann, Oliver, Budge, Kelly L., Cantarelli, Chiara, Fiaccadori, Enrico, Delsante, Marco, Morales, Enrique, Gutierrez, Eduardo, Niño-Cruz, Jose A., Martinez-Rueda, Armando J., Comai, Giorgia, Bini, Claudia, La Manna, Gaetano, Slon, Maria F., Manrique, Joaquin, Agraz, Irene, Sinaii, Ninet, and Cravedi, Paolo

Published
2021
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18. The long-acting C5 inhibitor, Ravulizumab, is effective and safe in adult patients with atypical hemolytic uremic syndrome naïve to complement inhibitor treatment

Author

Babu, Sunil, Broeders, Nilufer, Lietar, Nicole, Brown, Fiona, Campbell, Philip, Chowdhury, Paramit, Kasimatis, Theo, Cirami, Lino, Caroti, Leonardo, Antognoli, Guilia, Delmas, Yahsou, Dobronravov, Vladimir, Gaeckler, Anja, Garrouste, Cyril, Greenwood, Gregory, Griffin, Siân, Huang, Chiu-Ching, Chen, I-Ru, Huang, Susan, Kim, Jin Seok, La Manna, Gaetano, Comai, Giorgia, Cappuccilli, Maria, Le Quintrec, Moglie, Jeantet, Guillaume, Fumie, Iino, Luque, Yosu, Menne, Jan, Morelle, Johan, Goffin, Eric, Muhlfeld, Anja, Nagaraj, Shashi, Arepally, Gowthami, Oh, Doyeun, Okumi, Masayoshi, Terente, Manuel Praga, Gutierréz, Elena, Rodriguez, Paola, Provot, Francois, Schönermarck, Ulf, Fischereder, Michael, Terrada, Natalia Ramos, Seitz-Polski, Barbara, Favre, Guillaume, Boyer-Suavet, Sonia, Vinogradova, Maria, Kirsanova, Tatiana, Wong, Edwin K.S., Rondeau, Eric, Scully, Marie, Ariceta, Gema, Barbour, Tom, Cataland, Spero, Heyne, Nils, Miyakawa, Yoshitaka, Ortiz, Stephan, Swenson, Eugene, Vallee, Marc, Yoon, Sung-Soo, Kavanagh, David, and Haller, Hermann

Published
2020
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19. Prospective Study of Sars-cov2 Associated Coagulopathy and Role of Complement Activation

Author

Silingardi, Mauro, primary, Zappulo, Fulvia, additional, Dormi, Ada, additional, Pizzini, Attilia Maria, additional, Donadei, Chiara, additional, Fantoni, Chiara, additional, Zaccaroni, Stefania, additional, Pizzuti, Valeria, additional, Cilloni, Nicola, additional, Tantillo, Simona, additional, Guidi, Antonella, additional, Mancini, Rita, additional, Manna, Gaetano La, additional, and Comai, Giorgia, additional

Published
2024
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21. “Eculizumab first” in the management of post-transplant thrombotic microangiopathy.

Author

Maritati, Federica, primary, Corradetti, Valeria, additional, Bini, Claudia, additional, Provenzano, Michele, additional, Cuna, Vania, additional, Busutti, Marco, additional, Tondolo, Francesco, additional, Zappulo, Fulvia, additional, Vischini, Gisella, additional, Iacovella, Francesca, additional, Abenavoli, Chiara, additional, Borelli, Greta, additional, Demetri, Marcello, additional, Fabbrizio, Benedetta, additional, Radi, Giorgia, additional, Ravaioli, Matteo, additional, Mele, Caterina, additional, La Manna, Gaetano, additional, and Comai, Giorgia, additional

Published
2024
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25. Identification of asymptomatic Leishmania infection in patients undergoing kidney transplant using multiple tests

Author

Deni, Alessandro, primary, De Pascali, Alessandra Mistral, additional, Ortalli, Margherita, additional, Balducelli, Emma, additional, Provenzano, Michele, additional, Ferrara, Francesca, additional, Busutti, Marco, additional, La Manna, Gaetano, additional, Zammarchi, Lorenzo, additional, Bartoloni, Alessandro, additional, Caroti, Leonardo, additional, Ibarra-Meneses, Ana Victoria, additional, Carrillo, Eugenia, additional, Comai, Giorgia, additional, and Varani, Stefania, additional

Published
2023
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26. Histology for nephrology, from pre-implantation to post-transplant kidney biopsy. Lesson learned from ReBIrth (Renal BIopsy for Kidney Transplantation Therapy)

Author

Caliò, Anna, primary, Barreca, Antonella, additional, Marletta, Stefano, additional, Achenza, Maria Italia Sara, additional, Alessi, Marianna, additional, Angelico, Roberta, additional, Apicella, Luca, additional, Argiolas, Davide, additional, Bossini, Nicola, additional, Carrano, Rosa, additional, Carriero, Concetta, additional, Castellano, Giuseppe, additional, Comai, Giorgia, additional, Di Bella, Caterina, additional, D’Ignoto, Francesco, additional, Gallico, Agnese, additional, Gastaldon, Fiorella, additional, Merlotti, Guido, additional, Paloschi, Vera, additional, Panarese, Alessandra, additional, Parodi, Angelica, additional, Perna, Francesco, additional, Picciotto, Daniela, additional, Regalia, Anna, additional, Rossini, Michele, additional, Russo, Enrico, additional, Salerno, Maria Paola, additional, Toti, Luca, additional, Tulissi, Patrizia, additional, Vischini, Gisella, additional, Zaza, Gianluigi, additional, and Eccher, Albino, additional

Published
2023
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28. Immunosuppressive therapy and oral anticoagulation in kidney transplant recipients: Direct oral anticoagulants versus vitamin-k antagonists

Author

Santoro, Francesco, primary, Casanova, Annalisa, additional, Simone, Simona, additional, Alfieri, Carlo, additional, Falcone, Adele, additional, Dello Strologo, Andrea, additional, Grandinetti, Valeria, additional, Busutti, Marco, additional, Comai, Giorgia, additional, Marvulli, Tommaso Maria, additional, Zippo, Maria Grazia, additional, Castellano, Giuseppe, additional, La Manna, Gaetano, additional, Gesualdo, Loreto, additional, Giuseppe, Grandaliano, additional, and Pesce, Francesco, additional

Published
2023
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29. Safety and Efficacy of Vedolizumab in Kidney Transplant Recipients With Crohn’s Disease

Author

Busutti, Marco, primary, Baraldi, Olga, additional, Porcu, Clara Valentina, additional, Samele, Gianluca, additional, Campus, Anita, additional, Grandinetti, Valeria, additional, Bini, Claudia, additional, Provenzano, Michele, additional, Dussias, Nikolas, additional, Rizzello, Fernando, additional, Gionchetti, Paolo, additional, La Manna, Gaetano, additional, and Comai, Giorgia, additional

Published
2023
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30. Author Correction: Hypothermic Oxygenated New Machine Perfusion System in Liver and Kidney Transplantation of Extended Criteria Donors: First Italian Clinical Trial

Author

Ravaioli, Matteo, De Pace, Vanessa, Angeletti, Andrea, Comai, Giorgia, Vasuri, Francesco, Baldassarre, Maurizio, Maroni, Lorenzo, Odaldi, Federica, Fallani, Guido, Caraceni, Paolo, Germinario, Giuliana, Donadei, Chiara, Malvi, Deborah, Del Gaudio, Massimo, Bertuzzo, Valentina Rosa, Siniscalchi, Antonio, Ranieri, Vito Marco, D’Errico, Antonietta, Pasquinelli, Gianandrea, Morelli, Maria Cristina, Pinna, Antonio Daniele, Cescon, Matteo, and La Manna, Gaetano

Published
2020
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32. Anastomotic Pseudoaneurysm in Kidney Transplant: A Challenging Management for a Rare Entity.

Author

Maritati, Federica, Pini, Rodolfo, Comai, Giorgia, Bini, Claudia, Corradetti, Valeria, Mattiotti, Maria, Ravaioli, Matteo, Pini, Alessia, La Manna, Gaetano, and Gargiulo, Mauro

Abstract

Purpose: Anastomotic pseudoaneurysms of transplanted kidneys are a very rare complication encountered in less than 1% of cases. They may be devastating, leading to functional impairment, kidney transplantectomy, or death. Treatment has not been standardized, with open surgical repair considered the safest procedure even if it is often complicated by bleeding and graft loss. The purpose of this case report is to describe an endovascular treatment of this condition, consisting of the combination of coil embolization and arterial stenting. Case report: A 61-year-old woman developed an anastomotic pseudoaneurysm 2 months after kidney transplantation, causing acute kidney injury related to ab-extrinsic stenosis of the transplant renal artery (TRA) and external iliac artery. The pseudoaneurysm was successfully treated by coil embolization, and the arterial patency was restored by the stenting of TRA and external iliac artery. The patient completely recovered kidney function, and after a 6-month-follow-up, creatinine values were stable with normal renal perfusion. Conclusion: Endovascular repair through coil embolization and TRA stenting can be a safe and effective option to treat anastomotic pseudoaneurysm in kidney transplant. [ABSTRACT FROM AUTHOR]

Published
2024
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34. Relationship between immune response to SARS-CoV2 vaccines and development of breakthrough infection in solid organ transplant recipients: the CONTRAST cohort

Author

Bonazzetti, Cecilia, Tazza, Beatrice, Gibertoni, Dino, Pasquini, Zeno, Caroccia, Natascia, Fanì, Francesca, Fornaro, Giacomo, Pascale, Renato, Rinaldi, Matteo, Miani, Beatrice, Gamberini, Chiara, Morelli, Maria Cristina, Tamé, Mariarosa, Busutti, Marco, Comai, Giorgia, Potena, Luciano, Borgese, Laura, Salvaterra, Elena, Lazzarotto, Tiziana, Scudeller, Luigia, Viale, Pierluigi, Giannella, Maddalena, Bonazzetti, Cecilia, Tazza, Beatrice, Gibertoni, Dino, Pasquini, Zeno, Caroccia, Natascia, Fanì, Francesca, Fornaro, Giacomo, Pascale, Renato, Rinaldi, Matteo, Miani, Beatrice, Gamberini, Chiara, Morelli, Maria Cristina, Tamé, Mariarosa, Busutti, Marco, Comai, Giorgia, Potena, Luciano, Borgese, Laura, Salvaterra, Elena, Lazzarotto, Tiziana, Scudeller, Luigia, Viale, Pierluigi, and Giannella, Maddalena

Subjects
SARS-CoV2 infection, mRNA vaccine, antibody-response, COVID-19, serology, vaccination, solid organ transplantation, multistate model
Abstract

Background: SARS-CoV-2 vaccination in solid organ transplant (SOT) is associated with poorer antibody response (AbR) compared to non-SOT recipients. However, its impact on the risk of breakthrough infection (BI) should yet be assessed. Methods: Single-center prospective longitudinal cohort study enrolling adult SOT recipients who received SARS-CoV2 vaccination during 1-year period from February 2021, and followed-up to April 30th 2022. Patients were tested for AbR at multiple timepoints. Primary endpoint was BI (laboratory confirmed SARS-CoV2 infection ≥14 days after 2nd dose). Immunization (positive AbR) was considered an intermediate state between vaccination and BI. Probabilities of being in vaccination, immunization and BI states were obtained for each type of graft and vaccination sequence with multistate survival analysis, then multivariable logistic regression was performed to analyse the risk of BI in AbR levels. Results: 614 SOT (275 kidney, 163 liver, 137 heart, 39 lung) recipients were included. Most patients (84.7%) received three vaccine doses, the first two consisted of BNT162b2 and mRNA-1273 in 73.5% and 26.5% of cases, respectively; while at the third dose mRNA-1273 was administered in 59.8% of patients. Overall, 75.4% of patients reached immunization and 18.4% developed BI. Heart transplant recipients showed lowest probability of immunization (0.418) and highest of BI (0.323), all-mRNA-1273 vaccine-sequence showed higher probability of immunization (0.732) and lowest of BI (0.098). Risk of BI was higher for non-high-level AbR, younger age and shorter time from transplant. Conclusions: SOT patients with non-high-level AbR, shorter time from transplantation, and heart recipients are at highest risk of BI.

Published
2023

35. Circulating extracellular particles from severe COVID-19 patients show altered profiling and innate lymphoid cell-modulating ability

Author

Forte, Dorian, primary, Pellegrino, Roberto Maria, additional, Trabanelli, Sara, additional, Tonetti, Tommaso, additional, Ricci, Francesca, additional, Cenerenti, Mara, additional, Comai, Giorgia, additional, Tazzari, Pierluigi, additional, Lazzarotto, Tiziana, additional, Buratta, Sandra, additional, Urbanelli, Lorena, additional, Narimanfar, Ghazal, additional, Alabed, Husam B. R., additional, Mecucci, Cristina, additional, La Manna, Gaetano, additional, Emiliani, Carla, additional, Jandus, Camilla, additional, Ranieri, Vito Marco, additional, Cavo, Michele, additional, Catani, Lucia, additional, and Palandri, Francesca, additional

Published
2023
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36. Screening strategies for the diagnosis of asymptomatic Leishmaniainfection in dialysis patients as a model for kidney transplant candidates

Author

Comai, Giorgia, Mistral De Pascali, Alessandra, Busutti, Marco, Morini, Silvia, Ortalli, Margherita, Conte, Diletta, Re, Maria Carla, La Manna, Gaetano, and Varani, Stefania

Abstract

Despite being considered a tropical disease, visceral leishmaniasis (VL) caused by L. infantumis also endemic in the Mediterranean Europe and represents an increasing cause of morbidity and mortality in solid organ transplant (SOT) recipients. VL occurring in kidney transplant recipients is a severe event, often worsening the renal damage and leading to poor outcome. It is believed that most of VL cases in transplant recipients are caused by reactivation of a pre-existent, dormant leishmanial infection induced by the immunosuppressive drugs. Nevertheless, the prevalence of asymptomatic Leishmaniainfection in candidates to kidney transplant residing in or visiting endemic areas is unknown. As L. infantumis highly circulating in northeastern Italy, we aimed to examine the occurrence of this parasitic infection in 119 dialysis patients living in the mentioned area, 71 of whom were potential candidates to kidney transplant. By employing a combination of sensitive serological and molecular methods, we observed a prevalence of 15.9% asymptomatic Leishmaniainfection in the study cohort. This finding emphasizes the need of further evaluating potential screening strategies for Leishmaniainfection in solid organ transplant candidates residing in or visiting endemic areas.

Published
2024
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38. Regulatory T cells from patients with end-stage organ disease can be isolated, expanded and cryopreserved according good manufacturing practice improving their function

Author

Ulbar, Francesca, Montemurro, Tiziana, Jofra, Tatiana, Capri, Miriam, Comai, Giorgia, Bertuzzo, Valentina, Lavazza, Cristiana, Mandelli, Alessandra, Viganò, Mariele, Budelli, Silvia, Bacalini, Maria Giulia, Pirazzini, Chiara, Garagnani, Paolo, Giudice, Valeria, Sollazzo, Daria, Curti, Antonio, Arpinati, Mario, La Manna, Gaetano, Cescon, Matteo, Pinna, Antonio Daniele, Franceschi, Claudio, Battaglia, Manuela, Giordano, Rosaria, Catani, Lucia, and Lemoli, Roberto Massimo

Published
2019
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39. Renal Vessel Extension With Cryopreserved Vascular Grafts: Overcoming Surgical Pitfalls in Living Donor Kidney Transplant

Author

Fallani, Guido, primary, Maroni, Lorenzo, additional, Bonatti, Chiara, additional, Comai, Giorgia, additional, Buzzi, Marina, additional, Cuna, Vania, additional, Vasuri, Francesco, additional, Caputo, Francesca, additional, Prosperi, Enrico, additional, Pisani, Federico, additional, Pisillo, Beatrice, additional, Maurino, Ludovica, additional, Odaldi, Federica, additional, Bertuzzo, Valentina Rosa, additional, Tondolo, Francesco, additional, Busutti, Marco, additional, Zanfi, Chiara, additional, Del Gaudio, Massimo, additional, La Manna, Gaetano, additional, and Ravaioli, Matteo, additional

Published
2023
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41. sj-docx-1-jet-10.1177_15266028231162258 – Supplemental material for Renal Benefits of CO2 as a Contrast Media for EVAR Procedures: New Perspectives on 1 Year Outcomes

Author

Busutti, Marco, Sensoni, Alice, Vacirca, Andrea, Abenavoli, Chiara, Donadei, Chiara, Croci Chiocchini, Anna Laura, Righini, Matteo, Comai, Giorgia, Pini, Alessia, Faggioli, Gianluca, Gallitto, Enrico, La Manna, Gaetano, and Gargiulo, Mauro

Subjects
FOS: Clinical medicine, Cardiology, Medicine, 110323 Surgery
Abstract

Supplemental material, sj-docx-1-jet-10.1177_15266028231162258 for Renal Benefits of CO2 as a Contrast Media for EVAR Procedures: New Perspectives on 1 Year Outcomes by Marco Busutti, Alice Sensoni, Andrea Vacirca, Chiara Abenavoli, Chiara Donadei, Anna Laura Croci Chiocchini, Matteo Righini, Giorgia Comai, Alessia Pini, Gianluca Faggioli, Enrico Gallitto, Gaetano La Manna and Mauro Gargiulo in Journal of Endovascular Therapy

Published
2023
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42. Circulating extracellular particles from severe COVID-19 patients show altered profiling and innate lymphoid cell-modulating ability

Author

Forte, Dorian, Pellegrino, Roberto Maria, Trabanelli, Sara, Tonetti, Tommaso, Ricci, Francesca, Cenerenti, Mara, Comai, Giorgia, Tazzari, Pierluigi, Lazzarotto, Tiziana, Buratta, Sandra, Urbanelli, Lorena, Narimanfar, Ghazal, Alabed, Husam B. R., Mecucci, Cristina, La Manna, Gaetano, Emiliani, Carla, Jandus, Camilla, Ranieri, Vito Marco, Cavo, Michele, Catani, Lucia, Palandri, Francesca, Forte D., Pellegrino R.M., Trabanelli S., Tonetti T., Ricci F., Cenerenti M., Comai G., Tazzari P., Lazzarotto T., Buratta S., Urbanelli L., Narimanfar G., Alabed H.B.R., Mecucci C., La Manna G., Emiliani C., Jandus C., Ranieri V.M., Cavo M., Catani L., and Palandri F.

Subjects
SARS-CoV-2, lipidomic, Immunology, innate lymphoid cell, innate lymphoid cells, Immunology and Allergy, COVID-19, extracellular vesicles and particles, type 2 innate lymphoid cell, extracellular vesicles and particle
Abstract

IntroductionExtracellular vesicles (EVs) and particles (EPs) represent reliable biomarkers for disease detection. Their role in the inflammatory microenvironment of severe COVID-19 patients is not well determined. Here, we characterized the immunophenotype, the lipidomic cargo and the functional activity of circulating EPs from severe COVID-19 patients (Co-19-EPs) and healthy controls (HC-EPs) correlating the data with the clinical parameters including the partial pressure of oxygen to fraction of inspired oxygen ratio (PaO2/FiO2) and the sequential organ failure assessment (SOFA) score.MethodsPeripheral blood (PB) was collected from COVID-19 patients (n=10) and HC (n=10). EPs were purified from platelet-poor plasma by size exclusion chromatography (SEC) and ultrafiltration. Plasma cytokines and EPs were characterized by multiplex bead-based assay. Quantitative lipidomic profiling of EPs was performed by liquid chromatography/mass spectrometry combined with quadrupole time-of-flight (LC/MS Q-TOF). Innate lymphoid cells (ILC) were characterized by flow cytometry after co-cultures with HC-EPs or Co-19-EPs.ResultsWe observed that EPs from severe COVID-19 patients: 1) display an altered surface signature as assessed by multiplex protein analysis; 2) are characterized by distinct lipidomic profiling; 3) show correlations between lipidomic profiling and disease aggressiveness scores; 4) fail to dampen type 2 innate lymphoid cells (ILC2) cytokine secretion. As a consequence, ILC2 from severe COVID-19 patients show a more activated phenotype due to the presence of Co-19-EPs.DiscussionIn summary, these data highlight that abnormal circulating EPs promote ILC2-driven inflammatory signals in severe COVID-19 patients and support further exploration to unravel the role of EPs (and EVs) in COVID-19 pathogenesis.

Published
2023

43. Histology for nephrology, from pre-implantation to post-transplant kidney biopsy. Lesson learned from ReBIrth (Renal BIopsy for Kidney Transplantation Therapy)

Author

Caliò, Anna, Barreca, Antonella, Marletta, Stefano, Achenza, Maria Italia Sara, Alessi, Marianna, Angelico, Roberta, Apicella, Luca, Argiolas, Davide, Bossini, Nicola, Carrano, Rosa, Carriero, Concetta, Castellano, Giuseppe, Comai, Giorgia, Di Bella, Caterina, D'Ignoto, Francesco, Gallico, Agnese, Gastaldon, Fiorella, Merlotti, Guido, Paloschi, Vera, Panarese, Alessandra, Parodi, Angelica, Perna, Francesco, Picciotto, Daniela, Regalia, Anna, Rossini, Michele, Russo, Enrico, Salerno, Maria Paola, Toti, Luca, Tulissi, Patrizia, Vischini, Gisella, Zaza, Gianluigi, and Eccher, Albino

Subjects
whole-slide imaging, immunosuppression, kidney biopsy, kidney transplantation, digital pathology
Published
2023

46. Updated diagnosis and graft involvement for visceral leishmaniasis in kidney transplant recipients: a case report and literature review

Author

Busutti, Marco, primary, Deni, Alessandro, additional, De Pascali, Alessandra Mistral, additional, Ortalli, Margherita, additional, Attard, Luciano, additional, Granozzi, Bianca, additional, Fabbrizio, Benedetta, additional, La Manna, Gaetano, additional, Comai, Giorgia, additional, and Varani, Stefania, additional

Published
2022
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49. Identification of a serum and urine extracellular vesicle signature predicting renal outcome after kidney transplant

Author

Burrello, Jacopo, primary, Monticone, Silvia, additional, Burrello, Alessio, additional, Bolis, Sara, additional, Cristalli, Carlotta Pia, additional, Comai, Giorgia, additional, Corradetti, Valeria, additional, Grange, Cristina, additional, Orlando, Giuseppe, additional, Bonafè, Massimiliano, additional, La Manna, Gaetano, additional, Barile, Lucio, additional, and Bussolati, Benedetta, additional

Published
2022
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