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233 results on '"Coltro, Wendell K. T."'

13. Office paper and laser printing: a versatile and affordable approach for fabricating paper-based analytical devices with multimodal detection capabilities

Author

Sousa, Lucas R., primary, Guinati, Barbara G. S., additional, Maciel, Lanaia I. L., additional, Baldo, Thaisa A., additional, Duarte, Lucas C., additional, Takeuchi, Regina M., additional, Faria, Ronaldo C., additional, Vaz, Boniek G., additional, Paixão, Thiago R. L. C., additional, and Coltro, Wendell K. T., additional

Published
2024
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18. 3D PRINTING OF MAGNETIC SEPARATOR: AN AFFORDABLE APPROACH TO SAMPLE PREPARATION IN THE COVID-19 DIAGNOSIS

Author

Silva-Neto, Habdias A., Duarte-Junior, Gerson F., Bazílio, Gabriela Silvério, Parente-Rocha, Juliana Alves, Colugnati, Diego B., Borges, Clayton L., and Coltro, Wendell K. T.

Abstract

This report describes the fabrication of a low-cost magnetic separator holder combining 3D printing and compact neodymium blocks for allowing magnetic extraction and purification of RNA from samples collected by nasopharyngeal swab from patients infected by SARS-CoV-2. The device was designed to contain 24 entrances for plastic microtubes in an arrangement like a commercial device. The proof of concept of the proposed device was successfully demonstrated through the sample extraction and purification of swab samples collected from eight patients suspected of SARS-CoV-2 infection. The sample preparation protocol was performed using a commercial kit containing magnetic beads and different solutions. The performance of the printed device was compared to a commercial magnetic separator, usually employed in the golden standard techniques. The fabrication of the 3D printed magnetic separator was completed under optimized printing conditions within 6 h at cost of 4 USD per unit. The RNA extracted from samples using both devices was analyzed by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) and the achieved results have indicated no statistical different at confidence level of 95%. Based on the achievements, the use of 3D printing and neodymium blocks have demonstrated an alternative route to be used in routing analysis associated to COVID-19 diagnosis.

Published
2023

27. Direct immunoassay on a polyester microwell plate for colorimetric detection of the spike protein in swab and saliva samplesElectronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d3ay01755a

Author

Moreira, Nikaele S., Baldo, Thaisa A., Duarte, Lucas C., Lopes-Luz, Leonardo, Oliveira, Karoliny A., Estrela, Paulo F. N., Simões, Amanda M., Bührer-Sékula, Samira, Duarte, Gabriela R. M., and Coltro, Wendell K. T.

Abstract

This study presents the development of a polyester microplate for detecting the S-protein of the SARS-CoV-2 virus in saliva and nasopharyngeal swab samples using direct enzyme-linked immunosorbent assay (ELISA) technology. The polyester microplate was designed to contain 96 zones with a 3 mm diameter each, and a volume of 2–3 μL. The experimental conditions including reagent concentration and reaction time were optimized. The microplate image was digitized and analyzed using graphical software. The linear range obtained between protein S concentrations and pixel intensity was 0–10 μg mL−1, with a correlation coefficient of 0.99 and a limit of detection of 0.44 μg mL−1. The developed methodology showed satisfactory intraplate and interplate repeatability with RSD values lower than 7.8%. The results achieved through immunoassay performed on polyester microplates were consistent with those of the RT-PCR method and showed a sensitivity of 100% and 90% and specificity of 85.71% and 100% for saliva and nasopharyngeal samples, respectively. The proposed direct immunoassay on polyester microplates emerges as an alternative to conventional immunoassays performed on commercial polystyrene plates, given the low cost of the device, low consumption of samples and reagents, lower waste generation, and shorter analysis time. Moreover, the immunoassay has shown great potential for diagnosing COVID-19 with precision and accuracy.

Published
2023
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28. Distance-based detection of paracetamol in microfluidic paper-based analytical devices for forensic applicationElectronic supplementary information (ESI) available: Experimental optimization and comparison with reference technique. See DOI: https://doi.org/10.1039/d3ay01739g

Author

Moreira, Nikaele S., Pinheiro, Kemilly M. P., Sousa, Lucas R., Garcia, Gabriel D. S., Figueredo, Federico, and Coltro, Wendell K. T.

Abstract

Whisky adulteration is a prevalent practice driven by the high cost of these beverages. Counterfeiters commonly dilute whisky with less expensive alcoholic beverages, water, food additives, drugs or pharmaceuticals. Paracetamol (PAR), an analgesic drug that mitigates hangovers and headaches, is commonly used to adulterate whisky. Currently, the primary method for quantifying PAR levels is high-performance liquid chromatography, but this technique is both time consuming and usually generates more residues. In this context, the utilization of miniaturized and portable analytical devices becomes imperative for conducting point-of-care/need analyses. These devices offer several advantages, including portability, user-friendliness, low cost, and minimal material wastage. This study proposes the selective distance-based PAR quantification on whisky samples using a paper-based microfluidic analytical device (μPAD). Colorimetric detection on paper-based platforms offers great benefits such as affordability, portability, and the ability to detect PAR without complicated instrumentation. The optimal detection conditions were achieved by introducing 5 μL of a mixture containing 7.5 mmol L−1of Fe(iii) and K3[Fe(CN)6] into the detection zone, along with 12 μL of whisky samples into the sample zone. The method exhibited linear behavior within the concentration range from 15 to 120 mg L−1, with a determination coefficient of 0.998. PAR was quantified in adulterated samples. The results obtained with the paper-based devices were compared with a referenced method, and no significant differences were observed at a confidence level of 95%. The μPAD allowed to determine ca.1 drop of pharmaceutical medicine PAR of 200 mg mL−1in 1 L of solution, demonstrating excellent sensitivity. This method offers cost-effective and rapid analysis, reducing the consumption of samples, reagents, and wastes. Consequently, it could be considered a viable and portable alternative for analyzing beverages at criminal scenes, customs, and police operations, thereby enhancing the field of forensics.

Published
2023
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42. Cover Feature: Detection of Analgesics and Sedation Drugs in Whiskey Using Electrochemical Paper-based Analytical Devices (Electroanalysis 10/2018)

Author

Dias, Anderson A., primary, Cardoso, Thiago M. G., additional, Chagas, Cyro L. S., additional, Oliveira, Virgílio X. G., additional, Munoz, Rodrigo A. A., additional, Henry, Charles S., additional, Santana, Mário H. P., additional, Paixão, Thiago R. L. C., additional, and Coltro, Wendell K. T., additional

Published
2018
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47. Fast determination of cocaine and some common adulterants in seized cocaine samples by capillary electrophoresis with capacitively coupled contactless conductivity detection

Author

Marra, Mariana C., primary, de Castro Costa, Brenda M., additional, Munoz, Rodrigo A. A., additional, Santana, Mário H. P., additional, Maldaner, Adriano O., additional, Botelho, Élvio D., additional, Coltro, Wendell K. T., additional, and Richter, Eduardo M., additional

Published
2018
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