Search

The across-shore transport of meroplanktonic larvae is predominantly driven by coastal physical processes, resulting in episodic recruitment of benthic species. Historically, due to the sampling challenges associated with resolving these advective mechanisms across the continental shelf, relevant components of larval transport have been difficult to isolate and understand. We use three-dimensional temperature and velocity data from an array of 29 moorings to identify fundamental physical processes that could have generated successful across-shore transport and settlement of meroplankton. The dense spatial and temporal sampling from this array allows us to use Lagrangian particle tracking to estimate the influences of wind conditions and the internal tide on the across-shore transport of planktonic larvae. Settlement was found to be episodic at all depths studied. Above mid-water, modeled larvae were successfully transported onshore by the internal tide during wind relaxations. Surprisingly, abundant pulses of shallow-water larvae were supplied to the coast on occasions when strong, upwelling-favorable winds (> 4 m s−1) drove offshore-flowing surface waters, revealing a complex, potentially topographically influenced flow. These intense upwelling-favorable winds also contributed to subsurface onshore flows that created large pulses of larval settlement in deeper waters (> 20 m). Our analyses from this highly resolved data set provide novel insights into the interactions of physical drivers in creating episodic pulses of coastal larval recruitment.

An 18-month deployment of moored sensors in Iceland Basin allows characterization of near-inertial (frequencies near the Coriolis frequency f with periods of ~14 h) internal gravity wave generation and propagation in a region with an active mesoscale eddy field and strong seasonal wind and heat forcing. The seasonal cycle in surface forcing deepens the mixed layer in winter and controls excitation of near-​inertial energy. The mesoscale eddy field modulates near-inertial wave temporal, horizontal, and vertical scales, as well as propagation out of the surface layer into the deep permanent pycnocline. Wind-forced near-inertial energy has the most active downward propagation within anticyclonic eddies. As oceanic surface and bottom boundaries act to naturally confine the propagation of internal waves, the vertical distribution of these waves can be decomposed into a set of “standing” vertical modes that each propagate horizontally at different speeds. The lowest modes, which propagate quickly away from their generation sites, are most enhanced when the mixed layer is deep and are generally directed southward.

When the barotropic tide encounters variable bathymetry, fluctuating flow along a topographic slope generates baroclinic tides, or internal tides. There is growing evidence that these internal tides can affect primary production in the euphotic zone, though the dominant mechanisms are unclear. Internal tides move passive phytoplankton through an exponentially varying light field, enhancing primary production near the base of the euphotic zone. In addition internal tides also increase primary production through vertical nutrient advection into the euphotic zone. Topographically generated internal tides can be separated into two regimes: 1) the often highly nonlinear near-field regime where tidal beams are observed and 2) the more linear far-field regime. This study examines the primary production response to these internal tide processes using the Regional Ocean Modeling System (ROMS) coupled to a simple Nutrient, Phytoplankton, Zooplankton, Detritus (NPZD) model configured for an oligotrophic system with the nutricline positioned below 50 m depth. These idealized simulations generate internal tide beams with an oscillating, horizontal body force at the M2 tidal frequency that is applied to domains with a bathymetric step and uniform stratification. Sensitivity of the primary production response to the energy content of the tidal beam is obtained by adjusting the height and slope of the bathymetric step. Simulation results reveal that primary production intensifies along tidal beams due to the local enhancement of parcel vertical displacement (light effect) and nutrient advective flux divergence (nutrient effect). In the near-field regime across the range of step heights and slopes in this study, the nutrient effect is an order of magnitude larger and explains 92% of the variance in primary production versus only 14% for the light effect. The geometry of the generating feature sets the kinematics of the tidal beam. The light effect is limited in the euphotic zone across our domains because realized changes in light experienced over a tidal cycle are small relative to the amount of light available at a particular depth. In contrast, the magnitude of the nutrient effect increases more substantially with tidal beam energy.

There is strong interest in sustainable aviation fuels (SAF) to decarbonize aviation; however, local decision-makers will need to consider what additional incentives could stimulate SAF commercialization within their own jurisdictions. This study analyzed SAF production in Virginia, evaluating two biomass-to-energy platforms (gasification Fischer Tropsch [GFT] and pyrolysis) and two regionally abundant feedstocks (woody wastes and municipal solid wastes). A suite of open-access modeling tools were applied to possible SAF supply chains encompassing feedstock collection and transportation, conversion, and fuel upgrading and transport. Key modeling outputs were minimum product selling price (MPSP) ($/gallon) and life-cycle global warming potential (GWP) (g CO2eq/MJ). Results suggest that early SAF production via GFT will require local incentives of approximately $3.61 per gallon compared to $0.75 per gallon for pilot-scale pyrolysis. Location of production facility (by county) influences economic and environmental metrics but is not nearly as important as facility size (tonnes/year). Different formats of financial incentives (i.e., tax credits, loan forgiveness, etc.) offer markedly different reductions in SAF MPSP. Finally, under current federal incentives in the US, it is still more economically efficient to use pyrolysis (with higher GWP) than GFT (with lower GWP). Therefore, regional stakeholders will need to navigate the tradeoff between economic and environmental performances of these platforms. Though Virginia was used as a case study, the methodology is replicable for other jurisdictions, insofar it can be adapted for use in other locations without decision-makers having to completely build their own TEA models.

Background: Patella instability is one of the most common knee injuries in the adolescent patient. There are several pathoanatomic risk factors which should be assessed via several modalities, including X-rays, magnetic resonance imaging (MRI), or even CT scan. Objectives: We intend to review these risk factors along with the nonsurgical and surgical techniques used to prevent recurrent dislocations. Methods: We completed an extensive review of the recent literature concerning pediatric and adolescent patellar dislocation and subsequent treatment modalities. Results: We review in detail the risk factors such as patella alta, trochlear dysplasia, lateralization of the tibial tubercle or medialization of the trochlear groove (increased tibial tubercle to trochlear groove (TT–TG) distance), lower limb malalignment, excessive femoral anteversion and/or tibial torsion, and hyperlaxity. There are classification systems for dislocators, and a natural progression of instability that patients often proceed through. Only after a patient has continued to dislocate after bracing and physical therapy is surgical treatment considered. Surgical techniques vary, with the workhorse being the medial patellofemoral ligament (MPFL) reconstruction. However, there are a variety of other techniques which add onto this procedure to address other anatomic risk factors. These include the tibial tubercle osteotomy to address a large TT–TG distance or trochleoplasty to address the lack of a trochlear groove. Conclusions: Nonsurgical and surgical treatments for patella dislocators are tailored to the pathoanatomic risk factors in each patient.

(1) Background: The rise in antibiotic resistant bacteria poses a significant threat to public health worldwide, necessitating innovative solutions. This study explores the role of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) in the context of antibiotic resistance among different species from the Enterococcus genus. (2) Methods: The genomes of Enterococcus included in the study were analyzed using CRISPRCasFinder to distinguish between CRISPR-positive (level 4 CRISPR) and CRISPR-negative genomes. Antibiotic resistance genes were identified, and a comparative analysis explored potential associations between CRISPR presence and antibiotic resistance profiles in Enterococcus species. (3) Results: Out of ten antibiotic resistance genes found in Enterococcus species, only one, the efmA gene, showed a strong association with CRISPR-negative isolates, while the others did not significantly differ between CRISPR-positive and CRISPR-negative Enterococcus genomes. (4) Conclusion: These findings indicate that the efmA gene may be more prevalent in CRISPR-negative Enterococcus genomes, and they may contribute to a better understanding of the molecular mechanisms underlying the acquisition of antibiotic resistance genes in Enterococcus species.

Yokenella regensburgei is a Gram-negative rod part of the Enterobacteriaceae family (order Enterobacterales) and a rare cause of human infections. Although improved diagnostic methods have led to an increase in reports of this elusive pathogen, information remains limited. In order to provide a better understanding of this bacterium, we developed the first comprehensive review of its biology, biochemical profile, antimicrobial resistance pattern, virulence factors, natural reservoir and involvement in various veterinary and human infections. Human infections with this bacterium are scarcely reported, most probably due to constraints regarding its identification and biochemical similarities to Hafnia alvei. Multiple systematic searches revealed 23 cases of human infection, with a seemingly worldwide distribution, mostly in middle-aged or elderly male patients, often associated with immunosuppression. To date, Y. regensburgei has been reported in skin and soft tissue infections, bacteremia and sepsis, osteoarticular infections and in others such as urinary tract and digestive infections. The unique ability of Y. regensburgei to degrade polystyrene presents a novel and promising avenue for addressing plastic pollution in the near future. However, large-scale applications of this bacterium will undoubtedly increase human exposure, highlighting the necessity for comprehensive research into its role in human and veterinary infections, pathogenicity and antibiotic resistance.

When the barotropic tide encounters variable bathymetry, fluctuating flow along a topographic slope generates baroclinic tides, or internal tides. There is growing evidence that these internal tides can affect primary production in the euphotic zone, though the dominant mechanisms are unclear. Internal tides move passive phytoplankton through an exponentially varying light field, enhancing primary production near the base of the euphotic zone. In addition internal tides also increase primary production through vertical nutrient advection into the euphotic zone. Topographically generated internal tides can be separated into two regimes: 1) the often highly nonlinear near-field regime where tidal beams are observed and 2) the more linear far-field regime. This study examines the primary production response to these internal tide processes using the Regional Ocean Modeling System (ROMS) coupled to a simple Nutrient, Phytoplankton, Zooplankton, Detritus (NPZD) model configured for an oligotrophic system with the nutricline positioned below 50 m depth. These idealized simulations generate internal tide beams with an oscillating, horizontal body force at the M2 tidal frequency that is applied to domains with a bathymetric step and uniform stratification. Sensitivity of the primary production response to the energy content of the tidal beam is obtained by adjusting the height and slope of the bathymetric step. Simulation results reveal that primary production intensifies along tidal beams due to the local enhancement of parcel vertical displacement (light effect) and nutrient advective flux divergence (nutrient effect). In the near-field regime across the range of step heights and slopes in this study, the nutrient effect is an order of magnitude larger and explains 92% of the variance in primary production versus only 14% for the light effect. The geometry of the generating feature sets the kinematics of the tidal beam. The light effect is limited in the euphotic zone across our domains because realized changes in light experienced over a tidal cycle are small relative to the amount of light available at a particular depth. In contrast, the magnitude of the nutrient effect increases more substantially with tidal beam energy.

The relationship between fungal species and their resistance patterns in vineyard soils has important implications for agriculture and medicine. This study explored the prevalence of Aspergillus section Fumigati species and their resistance to azole compounds in Romanian vineyard soils. Methods: A total of 265 soil samples from various Romanian vineyards were screened for fungi resistant to azoles. Results: Aspergillus section Fumigati isolates exhibited significant resistance to itraconazole and voriconazole, but no azole-resistant Aspergillus fumigatus strains were detected. Six percent of the samples were positive for Aspergillus section Fumigati strains, all of which were azole-resistant. The strains were mainly Aspergillus udagawae (93.75%) and Aspergillus lentulus (6.25%). The predominant azole-resistant Aspergillus species were Aspergillus section Nigri strains, which were found in 75 soil samples. Conclusions: This study highlights the importance of understanding fungal resistance in vineyard soils for both the agricultural and clinical sectors. The presence of resistant strains may affect vine health and wine production while also constituting a challenge in the selection of effective treatments against severe and potentially fatal fungal infections in humans, stressing the importance of species-specific antifungal resistance knowledge.

The role of actin filaments in clathrin-mediated endocytosis (CME) is unclear. Here, Yang et al. show that branched actin filaments promote CME by pushing on clathrin coat edges in an epsin-dependent manner, dividing large flat clathrin plaques into sizes that facilitate invagination.

Recombinant adeno-associated virus (AAV) is an effective platform for therapeutic gene transfer; however, tissue-tropism differences between species are a challenge for successful translation of preclinical results to humans. We evaluated the use of in vitro primary hepatocyte cultures to predict in vivo liver-directed AAV expression in different species. We assessed whether in vitro AAV transduction assays in cultured primary hepatocytes from mice, nonhuman primates (NHPs), and humans could model in vivo liver-directed AAV expression of valoctocogene roxaparvovec (AAV5-hFVIII-SQ), an experimental gene therapy for hemophilia A with a hepatocyte-selective promoter. Relative levels of DNA and RNA in hepatocytes grown in vitro correlated with in vivo liver transduction across species. Expression in NHP hepatocytes more closely reflected expression in human hepatocytes than in mouse hepatocytes. We used this hepatocyte culture model to assess transduction efficacy of a novel liver-directed AAV capsid across species and identified which of 3 different canine factor VIII vectors produced the most transgene expression. Results were confirmed in vivo. Further, we determined mechanisms mediating inhibition of AAV5-hFVIII-SQ expression by concomitant isotretinoin using primary human hepatocytes. These studies support using in vitro primary hepatocyte models to predict species translatability of liver-directed AAV gene therapy and improve mechanistic understanding of drug-drug interactions.

Little evidence has been published regarding the antimicrobial resistance patterns of Helicobacter pylori (H. pylori) strains in Northwestern and Central Romania. The aim of this study was to determine the antibiotic resistance pattern of H. pylori isolates from gastric biopsies collected from patients living in Romania using ETEST® and GenoType HelicoDR. Gastric biopsies were obtained from 148 adult patients, 87 women and 61 men, the majority (131 patients) from Northwestern and Central Romania. Sixty-nine H. pylori strains were detected by both culture and PCR; sixty-three biopsies were negative by both techniques; one biopsy was positive by culture but negative by PCR; and fifteen biopsies were negative by culture but positive by PCR. Primary resistance against clarithromycin, fluoroquinolones, and metronidazole was found in 16.7%, 11.1%, and 13.3% of strains, respectively. No primary resistance has been detected against amoxicillin, tetracycline, and rifampicin. Secondary resistance against clarithromycin, fluoroquinolones, metronidazole, amoxicillin, tetracycline, and rifampicin was found in 75.8%, 30.3%, 65.5%, 1.8%, 1.8%, and 7.3% of the strains, respectively. The most frequent clarithromycin-resistant genotype detected by GenoType HelicoDR was A2147G (62.3%). Concordances between ETEST® and PCR for clarithromycin and fluoroquinolones were 85.5% and 78.3%, respectively. Further investigation of H. pylori resistance should be conducted to ensure proper eradication schemes.

The construction technique of raw earth, which has always been in use in most of the world, has left large monuments or architectural complexes to cultural heritage that need special attention due to the notable vulnerability of the material. A convenient way to deal this threat, besides physical intervention, is by using an information system, such as HBIM (Heritage Building Information Modeling), as a tool for damage assessment and conservation planning. This paper reports on its application in an archaeological setting, in particular, on the Huaca Arco Iris, a religious building of the old city of Chan Chan (Peru), the largest monumental complex in mud on the American continent. The study is part of the bilateral international project between the Consiglio Nazionale delle Ricerche (CNR) and the Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica (CONCYTEC) in the use of HBIM for the prediction of possible natural or anthropogenic damages to buildings in raw mud. Exploiting the data coming from the direct and indirect analyses, a dedicated ontology is built to guide the management of these data within the information system. The creation of an HBIM system for the archaeological domain, based on the trinomial data–information–knowledge, is presented and validated. Following this approach, a customizable HBIM has been created with the 3D model of the spatial entities of the Huaca. As a result, the semantic relationship of an external wall, taken as the benchmark test of our experiment, with the contained bas-relief and the conservation cover is tested.

Assessing bone density in prospective dental implant sites is crucial both for choosing the implant type and for planning a drilling procedure that will ensure the implant’s primary stability and osseointegration. This study aimed to investigate possible differences between the bone densities of various edentulous sites in the maxilla and mandible. The study was conducted on a group of forty partly edentulous patients who underwent radiological examination by scanning the areas of interest using cone beam computed tomography (CBCT). Hounsfield units (HU) were analyzed using dedicated software. Higher HU were observed at the site of mandibular central incisors compared to the site of maxillary central incisors. The HU values in the mandibular first molars region were higher than those of the maxillary first molars. Buccal vs. lingual or palatal cortical HU values did not differ significantly. Within the limitations of this study, it can be stated that an objective assessment of site-specific bone density before the installation of dental implants may provide valuable clinical information for the selection of implant size and the planning of a patient-specific drilling protocol.

Abstract In this work we present a unique set of coincident and collocated high‐resolution observations of surface currents and directional properties of surface waves collected from an airborne instrument, the Modular Aerial Sensing System, collected off the coast of Southern California. High‐resolution observations of near surface current profiles and shear are obtained using a new instrument, “DoppVis”, capable of capturing horizontal spatial current variability down to 128 m resolution. This data set provides a unique opportunity to examine how currents at scales ranging from 1 to 100 km modulate bulk (e.g., significant wave height), directional and spectral properties of surface gravity waves. Such observations are a step toward developing better understanding of the underlying physics of submesoscale processes (e.g., frontogenesis and frontal arrest) and the nature of transitions between mesoscale and submesoscale dynamics.

Background: Alternaria alternata is a melanic fungus capable of causing a wide variety of infections, some of which have lethal potential. It is a ubiquitous fungus and a well-known plant pathogen. Cutaneous infections with Alternaria alternata most often occur in the extremities of patients who perform conventional agriculture, thus being exposed to occupational hazards leading to the disruption of the skin barrier. Methods: This paper presents the first case report from Romania of an itraconazole nonresponsive cutaneous alternariosis in a patient without any type of immunosuppression. Results: After an initial misdiagnosis regarding the etiology of the patient’s skin infection, two successive punch biopsies, followed by mycologic examination, lead to the final diagnosis of cutaneous alternariosis. Treatment guided by antifungal susceptibility testing has been instituted, leading to the gradual healing of the patient’s skin ulcerations. Conclusions: The ability of Alternaria alternata to infect immunocompetent human hosts and to develop resistance to antifungal drugs highlight the importance of correctly diagnosing the etiology of skin ulcerations and instituting appropriate treatment guided by antifungal susceptibility testing whenever the suspicion of a fungal skin infection is plausible.

Sleep deprivation is highly prevalent in the modern world, possibly reaching epidemic proportions. While multiple theories regarding the roles of sleep exist (inactivity, energy conservation, restoration, brain plasticity and antioxidant), multiple unknowns still remain regarding the proposed antioxidant roles of sleep. The existing experimental evidence is often contradicting, with studies pointing both toward and against the presence of oxidative stress after sleep deprivation. The main goals of this review were to analyze the existing experimental data regarding the relationship between sleep deprivation and oxidative stress, to attempt to further clarify multiple aspects surrounding this relationship and to identify current knowledge gaps. Systematic searches were conducted in three major online databases for experimental studies performed on rat models with oxidative stress measurements, published between 2015 and 2022. A total of 54 studies were included in the review. Most results seem to point to changes in oxidative stress parameters after sleep deprivation, further suggesting an antioxidant role of sleep. Alterations in these parameters were observed in both paradoxical and total sleep deprivation protocols and in multiple rat strains. Furthermore, the effects of sleep deprivation seem to extend beyond the central nervous system, affecting multiple other body sites in the periphery. Sleep recovery seems to be characterized by an increased variability, with the presence of both normalizations in some parameters and long-lasting changes after sleep deprivation. Surprisingly, most studies revealed the presence of a stress response following sleep deprivation. However, the origin and the impact of the stress response during sleep deprivation remain somewhat unclear. While a definitive exclusion of the influence of the sleep deprivation protocol on the stress response is not possible, the available data seem to suggest that the observed stress response may be determined by sleep deprivation itself as opposed to the experimental conditions. Due to this fact, the observed oxidative changes could be attributed directly to sleep deprivation.

Abstract Anticipating the size of the next volcanic eruption in long-term forecasts is a major problem in both basic and applied volcanology. In this study, we investigate the extent to which eruption size is predictable based on historical and other attribute data. Data from the Smithsonian Global Volcanism Program (GVP) Catalog is used to determine the predictability of volcanic eruption size as quantified through the reported VEI (Volcano Explosivity Index). The numerical and categorical attributes from the global volcanic catalog were classified with trained random forest and simple prediction models to make a forecast of VEI that can be tested against the most recent eruption of each volcano. We compare these results to two different baseline predictability levels by: (a) selecting randomly from the global distribution of VEIs for the most recent eruptions to calculate a cohort baseline and (b) selecting the most frequent VEI for a given population to calculate a zero-rule baseline. We found that: (1) nearly any method that incorporates prior information on a specific volcano improves the prediction accuracy of the succeeding eruption VEI by at least 10 percentage points relative to the cohort baseline case, (2) incorporating attributes beyond previous VEIs can provide better accuracy and achieve up to 30 percentage point accuracy gains, (3) total accuracy of the VEI forecasting by these methods can be up to nearly 80% and (4) the zero-rule is an effective prediction method that is modestly outperformed (~ 5 percentage point gain) by random forest methods with multiple attributes on most datasets. We find no notable preference in accuracy based on volcano type. The results quantify the importance of volcano-specific information in long-term forecasting and may help practitioners assess their expected performance when anticipating future eruption size.

This study evaluated the safety and tolerability of ocular RS1 adeno-associated virus (AAV8-RS1) gene augmentation therapy to the retina of participants with X-linked retinoschisis (XLRS). XLRS is a monogenic trait affecting only males, caused by mutations in the RS1 gene. Retinoschisin protein is secreted principally in the outer retina, and its absence results in retinal cavities, synaptic dysfunction, reduced visual acuity, and susceptibility to retinal detachment. This phase I/IIa single-center, prospective, open-label, three-dose-escalation clinical trial administered vector to nine participants with pathogenic RS1 mutations. The eye of each participant with worse acuity (≤63 letters; Snellen 20/63) received the AAV8-RS1 gene vector by intravitreal injection. Three participants were assigned to each of three dosage groups: 1e9 vector genomes (vg)/eye, 1e10 vg/eye, and 1e11 vg/eye. The investigational product was generally well tolerated in all but one individual. Ocular events included dose-related inflammation that resolved with topical and oral corticosteroids. Systemic antibodies against AAV8 increased in a dose-related fashion, but no antibodies against RS1 were observed. Retinal cavities closed transiently in one participant. Additional doses and immunosuppressive regimens are being explored to pursue evidence of safety and efficacy (ClinicalTrials.gov: NCT02317887).

The aim of this study was to develop a suitable clotrimazole (CLT)-loaded mucoadhesive vaginal gel (CLT-MVG) for topical applications in vaginal candidiasis. Ten CLT-MVG formulations were prepared, consisting of mixtures of acid polyacrylic (Carbopol 940) and polyethene oxides, Sentry Polyox WSRN 1105 or 750, according to an experimental D-optimal design, and CLT was suspended at a ratio of 1%. The prepared CLT-MVG formulations were studied in vitro, and the formulation containing Carbopol 940 0.89% combined with PEO 1105 1.39% was identified with the optimal rheological and in vitro bioadhesion properties, ensuring the prolonged release of CLT, with a similarity factor greater than 50, indicating dissolution profile similarity for three batches of the optimized formulation. This optimized formulation showed a pH in the tolerance range, and an adequate ex vivo mucoadhesion time, while the FT-IR studies revealed no interactions between the excipients and CLT. The microscopic analysis identified a mean particle size of suspended CLT of 5.24 ± 0.57 μm. The in vitro antifungal activity of the optimized formulation was tested on twenty strains of Candida albicans and proved to be better compared to a marketed clotrimazole preparation, showing a greater inhibition effect (p < 0.05). The optimized formulation could be a good candidate for the local treatment of vaginal mycosis.

Dental 3D-printing technologies, including stereolithography (SLA), polyjet (triple-jetting technology), and fusion deposition modeling, have revolutionized the field of orthodontic occlusal splint manufacturing. Three-dimensional printing is now currently used in many dental fields, such as restorative dentistry, prosthodontics, implantology, and orthodontics. This study aimed to assess the mechanical properties of 3D-printed materials and compare them with the conventional polymethylmethacrylate (PMMA). Compression, flexural, and tensile properties were evaluated and compared between PMMA samples (n = 20) created using the “salt and pepper” technique and digitally designed 3D-printed samples (n = 20). The samples were subjected to scanning electron microscope analysis. Statistical analysis revealed that the control material (PMMA) exhibited a significantly higher Young’s modulus of compression and tensile strength (p < 0.05). In the flexural tests, the control samples demonstrated superior load at break results (p < 0.05). However, the 3D-printed samples exhibited significantly higher maximum bending stress at maximum load (MPa) (p < 0.05). Young’s modulus of tensile testing (MPa) was statistically significant higher for the control samples, while the 3D-printed samples demonstrated significantly higher values for elongation at break (p < 0.05). These findings indicate that 3D-printed materials are a promising alternative that can be effectively utilized in clinical practice, potentially replacing traditional heat-cured resin in various applications.

The goal of the current study was to determine the mechanical proprieties of polymethylmethacrylate (PMMA) and the improved compound, the graphene-based PMMA, with Zn and Ag and to compare the results. Scanning electron microscopy analysis of the samples before and after the mechanical test was conducted. The compression behavior, flexural properties, tensile strength, and shape of the samples were all investigated and compared between the variants of PMMA. Commercially available polymethylmethacrylate was used (Orthocryl®—Dentaurum, Ispringen, Germany) with the salt and pepper technique according to the manufacturer’s instructions to produce 20 samples for each mechanical trial with standard cylinders (4 mm diameter × 8 mm length) for compression, parallelepipedal prisms for flexing (2 mm × 2 mm × 25 mm) and flat samples for traction. There was no statistical difference in the mechanical proprieties of the samples evaluated, although there were values that could suggest significance. The graphene-based PMMA demonstrated good mechanical proprieties, like the commercially available PMMA, and appears promising for future clinical use based on its multiple advantages.

Enterococcus species are known for their ability to form biofilms, which contributes to their survival in extreme environments and involvement in persistent bacterial infections, especially in the case of multi-drug-resistant strains. This review aims to provide a comprehensive understanding of the mechanisms underlying biofilm formation in clinically important species such as Enterococcus faecalis and the less studied but increasingly multi-drug-resistant Enterococcus faecium, and explores potential strategies for their eradication. Biofilm formation in Enterococcus involves a complex interplay of genes and virulence factors, including gelatinase, cytolysin, Secreted antigen A, pili, microbial surface components that recognize adhesive matrix molecules (MSCRAMMs), and DNA release. Quorum sensing, a process of intercellular communication, mediated by peptide pheromones such as Cob, Ccf, and Cpd, plays a crucial role in coordinating biofilm development by targeting gene expression and regulation. Additionally, the regulation of extracellular DNA (eDNA) release has emerged as a fundamental component in biofilm formation. In E. faecalis, the autolysin N-acetylglucosaminidase and proteases such as gelatinase and serin protease are key players in this process, influencing biofilm development and virulence. Targeting eDNA may offer a promising avenue for intervention in biofilm-producing E. faecalis infections. Overall, gaining insights into the intricate mechanisms of biofilm formation in Enterococcus may provide directions for anti-biofilm therapeutic research, with the purpose of reducing the burden of Enterococcus-associated infections.

Abstract Background Giardia duodenalis is one of the most prevalent and highly diverse human parasites, encompassing a complex of eight genetically distinct assemblages, each further divided into sub-assemblages. While in recent years, G. duodenalis genotype distribution patterns in humans have been intensely studied, there is still very little information available on the diversity of Giardia genotypes and sub-assemblages infecting people in Romania. In the present study, we investigated the genetic diversity of Giardia duodenalis in asymptomatic patients from Romania. Methods Over an 11-month period, human feces from 7805 healthy adults were screened by microscopic analysis for G. duodenalis cysts during their obligatory periodic check-ups. DNA extraction was performed from microscopic-positive fecal samples, followed by multilocus sequence typing of four genetic loci of the ITS region, gdh, tpi and bg genes, followed by DNA sequencing and phylogenetic analysis. Statistical analysis was performed using EpiInfo 2000 software. Results The prevalence of giardiasis in the present study was 0.42% (33/7805). Twenty-three samples (76.67%) were successfully genotyped at each locus. The bg and tpi genes had the highest typing success rate (100%). The identified assemblages were assemblage A in 27 cases (subtypes A2 and A3), and B in 3 cases. Conclusions To our knowledge, the present study is the first report of multilocus sequence typing of G. duodenalis isolated from humans in Romania. The present results may shed light on G. duodenalis infection in humans at a regional and national level, thus increasing awareness against this parasitic infection.

Background and purposeBrain arteriovenous malformation (bAVM) is an important risk factor for intracranial hemorrhage. Current therapies are associated with high morbidities. Excessive vascular endothelial growth factor has been implicated in bAVM pathophysiology. Because soluble FLT1 binds to vascular endothelial growth factor with high affinity, we tested intravenous delivery of an adeno-associated viral vector serotype-9 expressing soluble FLT1 (AAV9-sFLT1) to alleviate the bAVM phenotype.MethodsTwo mouse models were used. In model 1, bAVM was induced in R26CreER;Eng2f/2f mice through global Eng gene deletion and brain focal angiogenic stimulation; AAV2-sFLT02 (an AAV expressing a shorter form of sFLT1) was injected into the brain at the time of model induction, and AAV9-sFLT1, intravenously injected 8 weeks after. In model 2, SM22αCre;Eng2f/2f mice had a 90% occurrence of spontaneous bAVM at 5 weeks of age and 50% mortality at 6 weeks; AAV9-sFLT1 was intravenously delivered into 4- to 5-week-old mice. Tissue samples were collected 4 weeks after AAV9-sFLT1 delivery.ResultsAAV2-sFLT02 inhibited bAVM formation, and AAV9-sFLT1 reduced abnormal vessels in model 1 (GFP versus sFLT1: 3.66±1.58/200 vessels versus 1.98±1.29, P

The widespread deployment of direct air capture is impeded by technology and scalability challenges, particularly resulting from high capital costs and energy demands. This paper posits that it is not possible to effectively evaluate the economic and environmental impacts of direct air capture without accounting for the spatial and temporal contexts in which it will be operated. Accordingly, the analysis aims to evaluate the near-term and medium-term (2030–50) potential of using excess renewable energy to power flexible deployment of direct air capture in California. Current systems would be able to sequester almost 1 million tonnes of carbon dioxide per year, an important benchmark target. For future systems, increased solar photovoltaic penetration could lead to potential carbon sequestration of 20–140 million tonnes during 2030–50. The environmental efficacy of excess electricity powered direct air capture is also estimated, which exhibits lower upstream energy consumption and net greenhouse gas emissions compared to the average range of carbon dioxide removal technologies. In terms of economic performance, the studied system is appealing across a wide range of capacity factors, thus showing large operational flexibility. Beyond this, the current study discusses a more fundamental premise, which is that different carbon dioxide removal approaches are uniquely suitable for different contexts that should be explicitly accounted for when evaluating their anticipated costs, benefits, and potential.

Severe cases of strongyloidiasis are most often associated with multiple causes of immune suppression, such as corticoid treatment and HTLV (human T-lymphotropic virus) coinfection. Diabetes is not traditionally considered a risk factor for the development of severe strongyloidiasis. We report a rare case of autochthonous severe strongyloidiasis in Romania, a European country with a temperate climate. A 71-year-old patient with no prior travel history was admitted with multiple gastrointestinal complaints and recent weight loss. CT (computed tomography) scans indicated duodenal wall thickening, and duodenal endoscopy evidenced mucosal inflammation, ulcerations and partial duodenal obstruction at D4. Microscopic examination of stool samples and biopsy specimens from the gastric and duodenal mucosa revealed an increased larval burden characteristic of Strongyloides stercoralis hyperinfection. Sequential treatment with albendazole and ivermectin achieved parasitological cure and complete recovery. The novelty of our case stems from the scarcity of severe strongyloidiasis cases reported in Europe and especially in Romania, the absence of other risk factors in our patient aside from diabetes, the involvement of the gastric mucosa and the rare presentation as partial duodenal obstruction. This case highlights the importance of considering strongyloidiasis as a differential diagnosis, even in temperate climates where cases are sporadic, in cases in which immune suppression is not evident and in the absence of eosinophilia. The case is presented in the context of the first literature review examining the relationship between severe strongyloidiasis and diabetes, emphasizing diabetes as a possible risk factor for severe strongyloidiasis.

DNA origami nanostructures (DOs) are promising tools for applications including drug delivery, biosensing, detecting biomolecules, and probing chromatin substructures. Targeting these nanodevices to mammalian cell nuclei could provide impactful approaches for probing, visualizing, and controlling biomolecular processes within live cells. We present an approach to deliver DOs into live-cell nuclei. We show that these DOs do not undergo detectable structural degradation in cell culture media or cell extracts for 24 hours. To deliver DOs into the nuclei of human U2OS cells, we conjugated 30-nanometer DO nanorods with an antibody raised against a nuclear factor, specifically the largest subunit of RNA polymerase II (Pol II). We find that DOs remain structurally intact in cells for 24 hours, including inside the nucleus. We demonstrate that electroporated anti-Pol II antibody-conjugated DOs are piggybacked into nuclei and exhibit subdiffusive motion inside the nucleus. Our results establish interfacing DOs with a nuclear factor as an effective method to deliver nanodevices into live-cell nuclei. [ABSTRACT FROM AUTHOR]

Abstract Background To assess color compatibility between dental structures (human enamel and dentine) and three different types of ceramic systems. Methods Samples (1 and 2 mm-thick) of extracted tooth (containing dentine and enamel areas) and three ceramic systems with different shades and opacities (HT–High Translucent, T–Translucent) were prepared for this study: Vita Suprinity—VS (HT, T; A1, A2, A3, A3.5, B2, C2, D2) (Vita Zahnfabrik); Vita Enamic—VE (HT, T; 1M1, 1M2, 2M2, 3M2) (Vita Zahnfabrik) and Noritake Super Porcelain EX-3—NKT (A1, A2, A3, A3.5, B2, C2, D2) (Kuraray Noritake Dental). Reflectance measurements of all samples were performed over black backgrounds using a non-contact spectroradiometer (SpectraScan PR-670, Photo Research) under a CIE 45°/0° geometry. CIE L*a*b* color parameters were measured and CIELAB/CIEDE2000 color differences (ΔE00/ΔE* ab) and corresponding Coverage Error (CE) of ceramic system for dentine or enamel samples were calculated. Color data was analyzed using one-way ANOVA and post-hoc multiple comparisons tests. CE values were interpreted by comparisons with available 50:50% acceptability color threshold (AT) for dentistry. Results Statistically significant differences in lightness were found among all ceramic systems and human dentine (p 0.05) with VEHT. Thin samples (1 mm) of dentine and enamel showed significant statistical differences (p

CSF HIV escape is a recently recognised phenomenon that suggests that despite suppressive treatment, HIV RNA may be detected in the CNS compartment in some individuals. In rare cases this is associated with clinical neurological disease, while in most case