1. Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2.
- Author
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Huerga Encabo H, Ulferts R, Sharma A, Beale R, and Bonnet D
- Subjects
- COVID-19 pathology, Hematopoietic Stem Cells pathology, Humans, Bone Marrow virology, COVID-19 virology, COVID-19 Nucleic Acid Testing methods, Colony-Forming Units Assay methods, Fetal Blood virology, Hematopoietic Stem Cells virology, SARS-CoV-2 isolation & purification
- Abstract
Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs, inlcuding procedures for SARS-CoV-2 production and titration, isolation of human HSPCs from different sources (bone marrow, umbilical cord, or peripheral blood), and quantification of SARS-Cov-2 infection capacity by RT-qPCR and colony forming unit assay. For complete details on the use and execution of this protocol, please refer to Huerga Encabo et al. (2021)., Competing Interests: The authors declare no competing interests., (© 2021 The Authors.)
- Published
- 2021
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