1. THE INFLUENCE OF A CANNULA IN THE RABBIT OVIDUCT
- Author
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Sloan Mh, Johnson Ad, and Coley Sl
- Subjects
Embryology ,medicine.medical_specialty ,animal structures ,business.industry ,medicine.medical_treatment ,media_common.quotation_subject ,Obstetrics and Gynecology ,Embryo ,Ovary ,Cell Biology ,Silastic ,Cannula ,Surgery ,Constriction ,Endocrinology ,medicine.anatomical_structure ,Reproductive Medicine ,Laparotomy ,medicine ,Oviduct ,business ,Ovulation ,media_common - Abstract
A study was undertaken to develop a practical technique for transferring ova to the oviducts of recipient rabbit does through an indwelling cannula. 28 sexually mature virgin does weighing between 3.5 and 5.5 kg were used. The operation to install the cannula and extraabdominal flask was performed with aseptic precautions. Amesthesia was induced by a slow injection of sodium pentobarbitone into the ear vein. An incision was made in the left flank approximately 3.5 cm from the loin midway between the ribs and hipbone. The ovary and oviduct were exposed through the incision. The silastic tube was inserted into the fimbriated end of the oviduct and sutured in place. The cannula was carried out through the incision and the extraabdominal flask which housed the external end of the cannula was sutured in place in the flank. Varying numbers of days after cannulation donors and recipients were mated and injected with HCG to ensure ovulation. The donors were anesthetized 48-52 hours after mating and mid-ventral laparotomy was performed. The oviducts were flushed and collected in a watch-glass. The ova were transferred from the watch-glass to the recipient. The ova pick-up tube was passed down the cannula to the fimbria and they were flushed into the oviduct. On Day 12 after transfer the recipients were subjected to mid-ventral laparotomy and normal developing embryos were counted. They were then allowed to go to term. In 18 does with no infection or constriction of the cannula 44.4% of the transfers resulted in implantation. 35 ova were transplanted to 8 recipients that showed implantation sites on Day 12 and 60% of these ova implanted. More successful transfers were carried out on the same rabbits. It is noted that control of genital infection and improvement of the technique could greatly improve implantation percentages.
- Published
- 1974
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