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The effects of diet composition and chemical form of Se on intestinal flow, absorption, and retention of Se were determined in sheep by the balance technique and by disappearance of Se from sites along the gastrointestinal tract with reference to dual-phase digesta markers. Six sheep with ruminal and duodenal cannulas were used in a crossover design with a split-plot arrangement of the Se isotope treatments. Sheep were fed a forage (alfalfa hay)based (.37 mg Se/kg) or concentrate (barley)-based (.27 mg Se/kg) diet at 90% of ad libitum intake. Selenium stable isotopes (enriched [77Se]yeast, enriched [82Se]selenite) and fluid (Co-EDTA) and particulate (Cr-mordanted fiber) markers were administered simultaneously into the rumen four times daily for 7 d, and total collections of feces and urine were made every 24 h for these and the following 7 d. A larger proportion (51 to 61%) of the Se tracers flowing to the duodenum was associated with the particulate fraction, mainly as bacteria-associated Se, than with the fluid fraction. The [82Se]selenite was more available (P < .05) for absorption and retention than [77Se]yeast, indicating that inorganic chemical forms of Se are as available to the ruminant as organic forms of Se commonly found in feedstuffs. Selenium absorption and retention were greater (P < .05) in sheep receiving the concentrate-based diet than in sheep receiving the forage-based diet. Thus, the availability of Se from inorganic and organic sources in sheep seems to be influenced by diet composition. Key Words: Selenium, Absorption, Retention, Stable Isotopes, Sheep

Alfalfa (Medicago sativa L.) is an important forage crop worldwide, but molecular genetics and breeding research in this species are hindered by its self-incompatibility (SI). Although the mechanisms underlying SI have been extensively studied in other plant families, SI in legumes, including alfalfa, remains poorly understood. Here, we determined that self-pollinated pollen tubes could germinate on the stigma of alfalfa, grow through the style, and reach the ovarian cavity, but the ovules collapsed ~48 h after self-pollination. A transcriptomic analysis of dissected pistils 24 h after self-pollination identified 941 differently expressed genes (DEGs), including 784 upregulated and 157 downregulated genes. A gene ontology (GO) analysis showed that the DEGs were highly enriched in functions associated with the regulation of pollen tube growth and pollen germination. A Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that pentose and glucuronate interconversion, plant hormone signal transduction, the spliceosome, and ribosomes might play important roles in SI. Our co-expression analysis showed that F-box proteins, serine/threonine protein kinases, calcium-dependent protein kinases (CDPKs), bHLHs, bZIPs, and MYB-related family proteins were likely involved in the SI response. Our study provides a catalog of candidate genes for further study to understand SI in alfalfa and related legumes. [ABSTRACT FROM AUTHOR]

It was assumed that the production of alfalfa in soils naturally poor in available nutrients, such as potassium (K) and calcium (Ca), depends on the use of fertilizers. This hypothesis was validated in an experiment with an alfalfa–grass mixture carried out in 2012, 2013 and 2014 on soil formed from loamy sand that had a low content of available Ca and K. The two-factor experiment consisted of two levels of applied gypsum as a source of Ca (0, 500 kg ha−1) and five levels of PK fertilizers (absolute control, P60K0, P60K30, P60K60 and P60K120). The total yield of the sward was determined by the main seasons of alfalfa–grass sward use. Gypsum application increased the yield by 1.0 t ha−1. The highest yield of 14.9 t ha−1 was obtained on the plot fertilized with P60K120. Based on the nutrient content in the sward, it was shown that the main yield predictor was the content of K in the first cut of sward use. The reliable yield predictors, based on the total accumulation of nutrients in the sward, turned out to be K, Mg and Fe. The nutritional quality of the alfalfa–grass fodder, based on the K/Ca + Mg ratio, depended mainly on the season of the sward use, which was substantially deteriorated by the K fertilizer. Gypsum did not control this process. The productivity of the nutrients taken up by the sward depended on the accumulated K. Its yield-forming effect was significantly limited by manganese deficiency. The use of gypsum positively affected the uptake of micronutrients, consequently increasing their unit productivity, especially of manganese. Optimization of the production of alfalfa–grass mixtures in soils poor in basic nutrients requires micronutrients to be taken into account. Their uptake by plants can be limited by high doses of basic fertilizers. [ABSTRACT FROM AUTHOR]

The aim of this study was to assess the effect of rubber seed kernel heat processing on in vitro rumen biohydrogenation of fatty acids and fermentation. The experiment was conducted with a completely randomized design (CRD). The inclusion of RSK at 0% (CON) and 20% with different processing methods as follows: Raw rubber seed kernel (RAWR), roasted rubber seed kernel (ROR), microwave irradiated rubber seed kernel (MIR), and rubber seed kernel were heated in a hot air oven (RHO) in total mixed ration (TMR) diets. The hydrogen cyanide (HCN) was reduced using RSK heat methods. The heat processing of RSK had no effect on cumulative gas production at 96 h, the gas production from the insoluble fraction (b), or degradability (p > 0.05), whereas it reduced the gas production from the immediately soluble fraction (a) and constant rate of gas production for the insoluble fraction (c) (p < 0.01). The RSK processing methods did not influence ruminal pH, total volatile fatty acid (VFA), or VFA proportions (p > 0.05). RSK heat processing reduced ammonia-nitrogen (NH3-N) (p < 0.04) while increasing the bacterial population (p < 0.02). Heat treatment had no effect on linoleic acid (C18:2 cis-9,12 + tran-9,12) (p > 0.05). The RHO increases oleic acid (C18:1 cis-9 + tran-9) and linolenic acid (C18:3 cis-9,12,15) concentrations (p < 0.01). In conclusion, RHO reduced rumen biohydrogenation of unsaturated fatty acids (UFA), especially C18:3 and C18:1. [ABSTRACT FROM AUTHOR]

Simple Summary: Scientists constantly seek techniques that may improve animal-feed usage, because they can reduce environmental impact and increase the profitability of feedlot systems. In beef-cattle production systems, high-performance animals need a greater protein supply that escapes rumen fermentation to be digested in the intestine compared to low-performance animals. This fraction is called rumen undegradable protein (RUP). Cottonseed meal is a possible protein source with an excellent amino-acid profile; however, cottonseed meal has low RUP content, which can be increased by applying heat and xylose. Thus, we submitted this feed to different heat techniques (autoclave, conventional, and microwave ovens), with and without xylose treatment, to increase its RUP content. Our results suggest that the evaluated processing methods may increase cottonseed meal RUP. The best treatments under the experimental conditions were: for the autoclave, xylose-treated cottonseed meal with 8 and 16 min heating; for conventional oven, 90 min heating for xylose-treated cottonseed meal; and for the microwave oven, xylose-treated cottonseed meals with 2, 4, and 6 min heating. Further studies are necessary to confirm the results found here and evaluate the effects of these processed ingredients on ruminal fermentation parameters, animal performance, and economic viability. The ruminal kinetics of protein sources may be changed by heat and sugar treatments. Thus, these processing methods may be used as alternatives to increase beef-cattle diets' rumen undegradable protein (RUP). We aimed to evaluate the effects of processing cottonseed meals with autoclave, conventional, and microwave ovens, with and without using xylose, on the ruminal kinetics degradation parameters and intestinal digestibility (ID). In situ studies were conducted, and each sample was incubated in the rumen to determine dry matter (DM) and crude protein (CP) rumen degradation kinetics. In vitro studies were also conducted to evaluate ID. The control treatment had a greater soluble fraction for DM and CP than processed cottonseed meals (p < 0.05). The addition of xylose decreased both DM and CP water-soluble fractions (fraction A) of cottonseed meal heated in a conventional oven (p < 0.05). Compared to the control, we observed a decrease in effective degradability and increased RUP for all processed methods (p < 0.05). Furthermore, conventional and microwave ovens showed greater ID than the control. Moreover, xylose-treated groups heated in the autoclave and conventional ovens had greater ID than xylose-untreated cottonseed meal. Under these experimental conditions, cottonseed RUP was increased by the evaluated processing methods. [ABSTRACT FROM AUTHOR]

The performance of farming systems models for grazed grasslands are seldom evaluated against comprehensive field data. The aim of this study was to evaluate the capacity of a daily time step, grazing systems simulation model—the SGS (Sustainable Grazing Systems) Pasture Model—to simulate production and aspects of sustainability. This was completed by evaluating temporal changes in soil water balance, some major nitrogen (N) fluxes, as well as plant and animal production using data from two large scale experimental sites with grazing sheep. The simulations were broadly in agreement with the measurements. In cases where divergence occurred the reasons were apparent and could be explained by reference to the model structure or aspects of the field data. In particular, the simulations showed good agreement with the observed soil water, but poorer agreement with the volumes of runoff. The simulated N in leachate and soil inorganic N were less in agreement with the measured data. The model outputs were sensitive to symbiotic biological fixation by subterranean (sub) clover and mineralisation of soil organic matter, which were not measured. Similarly, there were variable results for the simulation of animal growth and production. The complexities of simulating grazing systems and comparing field observations to simulated values are discussed. [ABSTRACT FROM AUTHOR]

Simple Summary: Rumination and activity behavior are important indices for monitoring the welfare and health status of beef cattle. Stress, excitement, and diseases can alter the rumination and activity patterns of beef cattle. Backgrounding allows producers to feed a variety of forages to improve growth performance before the beef calves enter the feedlot. This study was designed to evaluate the influences of sex, breed, and backgrounding diet on rumination, and the activity patterns in Angus and Angus-cross beef calves. Moreover, the daily variations in rumination and activity in Angus and Angus-cross beef calves under different backgrounding systems were studied. Our results demonstrated that the time of the day and backgrounding diet influenced rumination and activity patterns of Angus and Angus-cross beef calves. The objectives of this study were to evaluate the influences of sex, breed, and backgrounding diet on rumination and activity patterns in Angus and Angus-cross beef calves; and the daily variations in rumination and activity in Angus and Angus-cross beef calves under different backgrounding systems. A total of 62 freshly weaned calves were vaccinated and randomly stratified by sex (heifers and steers), breed (Angus and Angus × Simmental cross), and assigned randomly to 3 backgrounding treatments for 55 days. The peak values for rumination and activity in heifers, steers, Angus, and Angus × Simmental cross occurred during the dark and light phases of the dark/light cycle, respectively. Beef calves backgrounded on cover crops had higher (p < 0.05) rumination (45.33 ± 1.57 min) compared with calves backgrounded on a perennial pasture (43.96 ± 1.47 min) diet. Similarly, drylot calves (24.16 ± 0.68 min) had higher (p < 0.05) activity compared to perennial pasture (23.49 ± 0.72 min). The results showed that sex and breed did not influence rumination and activity of Angus and Angus-cross beef calves during the study period. We concluded that the time of the day and backgrounding diet influenced rumination and activity patterns of Angus and Angus-cross beef calves. [ABSTRACT FROM AUTHOR]

The effect of synergies between saline-tolerant grass and legume species grown in saline soil, on yield, quality, composition, persistence, and weed suppression was studied in 2019–2021 in a moderately saline soil located in the dark brown soil zone near Saskatoon, Saskatchewan, Canada. 'Halo' alfalfa (ALF), a salt-tolerant cultivar of alfalfa, was seeded in binary mixtures with 'Revenue' slender wheatgrass (ALF-SWG), 'Garrison' creeping meadow foxtail (ALF-CMF), and 'Radisson' smooth bromegrass (ALF-SBG). A complex mixture (quaternary) with all three grasses was also included. Four replicated treatments (n = 4) were randomly allocated to 6.2 × 1.2 m plots in spring 2019. The binary mixtures had similar or slightly better stand establishment compared to the quaternary mixture over the two years (83.3 vs. 76.9%), the quaternary stand showed greater (p = 0.01) establishment in Yr 2 than in Yr 1 (85 vs. 68.8%). There was high weed infestation in all the stands, but ALF-SWG had less weed infestation (23.8 vs. 44.1%) than ALF-CMF, especially in Yr 2 (p = 0.05). There were no significant differences among mixtures in yield, nutritional composition, and nutrient uptake. The ALF-CMF and ALF-SWG binary mixtures had 9 to 23% greater mean total DMY than the other mixtures in the moderately saline soil. The cost of establishing forage mixtures on unproductive saline land can be up to 89% recovered after only two years. The results suggested that binary mixtures of 'Halo' alfalfa with 'Revenue' slender wheatgrass or 'Garrison' creeping meadow foxtail could be reasonable alternatives for adequate forage production and quality, high N-use efficiency, and ultimately livestock gain per hectare, as well as for controlling soil salinity and improving soil fertility in this saline area in the dark brown soil zone. [ABSTRACT FROM AUTHOR]

Flavonoids are essential compounds widespread in plants and exert many functions such as defence, definition of organ colour and protection against stresses. In Medicago truncatula, flavonoid biosynthesis and accumulation is finely regulated in terms of tissue specificity and induction by external factors, such as cold and other stresses. Among flavonoids, anthocyanin precursors are synthesised in the cytoplasm, transported to the tonoplast, then imported into the vacuole for further modifications and storage. In the present work, we functionally characterised MtrGSTF7, a phi-class glutathione S-transferase involved in anthocyanin transport to the tonoplast. The mtrgstf7 mutant completely lost the ability to accumulate anthocyanins in leaves both under control and anthocyanin inductive conditions. On the contrary, this mutant showed an increase in the levels of soluble proanthocyanidins (Pas) in their seeds with respect to the wild type. By complementation and expression data analysis, we showed that, differently from A. thaliana and similarly to V. vinifera, transport of anthocyanin and proanthocyanidins is likely carried out by different GSTs belonging to the phi-class. Such functional diversification likely results from the plant need to finely tune the accumulation of diverse classes of flavonoids according to the target organs and developmental stages. [ABSTRACT FROM AUTHOR]

Spiny thickets or xerophytic thickets (XTs) are a type of shrubby vegetation found in the far south and southwest of Madagascar, the driest parts of the island. This type of vegetation, which is rich in endemic animal and plant species, is endangered. Extensive local goat breeding (Capra hircus, for meat and milk production) based on XT browsing is an important source of household income. The aim of this paper is to analyse the possibility of using improved goat breeding as an alternative to wood charcoal (WC) production and slash-and-burn agriculture (SBA), which are unsustainable activities. The literature on (i) the impacts of SBA, WC production, and goat browsing on the XT ecosystem and (ii) the income provided by these three activities is reviewed to determine the sustainability level of improved goat husbandry. SBA and WC production reduced XT biomass and XT cover area, while goat browsing alone, at a stocking rate of one head per hectare, did not affect XT leaf biomass production and shrub regeneration. Furthermore, batch breeding and flushing techniques provided the highest annual income, mainly from the sale of surplus animal products. This improved goat husbandry may be a sustainable alternative to WC production. However, increasing the current stocking rate is necessary to surpass the combined income of WC production and SBA. An estimation of the XT carrying capacity would offer a basis to assess whether this ecosystem would support a higher stocking rate. [ABSTRACT FROM AUTHOR]

Questions: Does plant composition differ among grasslands having divergent disturbance and management histories? Which vegetation attributes and disturbances specifically influence rangeland health? Location: Northern temperate pastures in the Central Parkland and adjacent Boreal natural regions of central Alberta, Canada. Methods: Plant composition and rangeland health data from 102 pastures were related to disturbance history acquired from retrospective producer surveys. Health assessments evaluated indicators such as vegetation composition, hydrologic function, and site stability. Producers were asked to identify pasture history (e.g., last cultivation date, whether fire had occurred), contemporary grazing actions (timing of use, grazing systems, type of livestock), and other management activities (i.e. herbicide application, manure spreading). Results: Cultivation and burn history were the primary drivers of vegetation differences, where previously cultivated pastures were dominated by introduced, naturalized grasses, eliminating most native species. Remnant non‐cultivated grasslands were occupied by a mix of native and invasive plant species. Grazing system had limited impact on vegetation composition due to uniformly high livestock stocking. Plant composition corresponded with gradients in rangeland health, where the latter declined with increased stocking rate and supplemental feeding on pasture. Greater health was characterized by increased cover of graminoids (primarily introduced forages), abundant litter, low plant richness, and reduced stocking rates. Other land management activities had comparatively less impact on the composition and health of these grasslands. Conclusions: Conversion of native grassland and forest in this region has a strong legacy effect on vegetation. Management actions associated with high stocking rates and supplemental feeding lowered rangeland health. [ABSTRACT FROM AUTHOR]

Core IdeasMethods to improving depleted forage stand by seeding, weed control, fertilizer application, and aeration were investigated.A combination of vegetation control + direct seeding improved forage yield, crude protein, and total digestible nutrients.A 2‐year combined forage yield for four methods increased profit from forage production. A 3‐year on‐farm study was conducted in northwestern Alberta to determine the effects of several pasture rejuvenation methods such as breaking and reseeding, spraying to control weeds and brush, forage‐seeding methods, fertilizer application, pasture rest, and aeration/spiking on forage dry matter (DM) yield, botanical composition, and forage quality. Three years after methods were implemented, the pasture rejuvenation methods investigated significantly affected (P < 0.05) forage DM yield; forage botanical composition; forage Ca, P, Ca:P ratio, K, Mg, and Cu; revenue generated for forage production; and marginal returns. Rejuvenation methods did not affect (P > 0.05) forage C:P ratio, S, Na, Fe, Zn, Mn, and total digestible nutrients. Spring herbicide application + direct seeding (RSS), fertilizer application (FERT), and fall herbicide application + broadcast seed in spring (RFBSS) improved forage production and some forage quality parameters more than other methods over control. Legume composition in the total forage production was as much as 29% for RSS compared with 2–17% for other methods including the control. For a 2‐year combined forage DM yield after methods were implemented, total forage DM yield, revenue generated, and marginal returns for forage production as well as profit over control were significantly greater (P < 0.05) for RSS than other pasture rejuvenation methods investigated. The study demonstrated that RSS improved forage DM yield and some quality parameters (particularly crude protein, Ca, and Ca:P). Also, RSS increased both revenue and profit over most methods including control. Results suggest RSS can be recommended as an effective method or option of improving the forage productivity of depleted pastures. [ABSTRACT FROM AUTHOR]

Mediterranean forage systems suffer from limited availability of fresh forage because of water deficits and extreme temperatures. Consequently, fresh forage is unavailable for at least 6-7 months a year, and farmers must buy feed to support livestock production. With the aim of overcoming these limitations, a 2-year trial was conducted on three distinct sites in Sicily (at 10, 600 and 1200 m elevation) with thirty-four varieties of forage species belonging to nine biennial/perennial and thirteen annual species. Results showed that by integrating grasses and legumes, species from environments with different climatic conditions enable the season of forage production to be extended from mid- April to mid- November. Quality traits of forage in different areas varied in relation to species and varieties. In general, the sown-forage quality was better than in pastures and fallows in the same areas commonly used to feed animals. This also leads to a reduction in the use of supplementary feeds. Among the tested species, Lolium multiflorum and Medicago sativa emerged as the most promising for filling the forage-deficit periods, and Trifolium spp. and Vicia sativa were found to be superior for increasing forage quality. The results are discussed in the context of adapting Mediterranean forage supplies for ensuring greater sustainability of livestock production in mountain, hill and plain areas. The proposed forage chain arrangement represents part of local potential adaptation to climate limitations and climate change. [ABSTRACT FROM AUTHOR]

Copyright of African Journal of Ecology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Selection of an appropriate forage species is an important first step in successful forage production. Among forage species, canola (Brassica napus L.) can be cut for hay or silage to cover certain costs of growing the crop. Because forage production is largely controlled by the environment and management, this experiment was conducted in a semiarid region of Iran during the 2006-2007 and 2008-2009 growing seasons to investigate whether canola forage yield and its nutritive value affected by different rates of N and natural zeolite. The experimental treatments were arranged in randomized complete blocks with three replications and comprised a factorial combination of three N levels (90,180, and 270 kg N ha-1) and four zeolite rates (0,3,6, and 91 zeolite ha-1 ). The results showed that the enhanced N application from 90 to 270 kg N ha-1 resulted in a 49% rise in forage yield for the first year and a 39% increase for the second year. The converse effects from N and zeolite yielded a significant interaction on the forage nitrogen concentration (FNC) so that the N and zeolite application enhancement led to an increase and decrease, respectively, in the FNC. The integration of the minimum N level with the maximum zeolite application generated the highest forage calcium concentration (FCC) (1.13% in dry matter). In contrast, N270Z0 treatment yielded the lowest FCC (0.54% in dry matter). There was a linear response between N application rate and its leaching loss whereas in the zeolite treatments, minimum N leaching was observed after the Z9 treatment. A combined application of zeolite and chemical N for canola production in a poor sandy soil is recommended to ensure an acceptable forage yield and for soil protection from excess N leaching loss. [ABSTRACT FROM AUTHOR]

To optimize management, farmers require quantitative understanding of the factors affecting variability in soybean [Glycine max (L.) Merr.] seed yield and quality. Our objectives were to characterize spatial variation in soybean seed yield, oil concentration, and protein concentration in two south-central Minnesota fields over 6 yr of a corn [Zea mays L.]-soybean rotation, and to determine the influence of fertilizer treatments, soil chemical properties, and topography on soybean yield, oil, and protein. Soil and topographical variables were observed on 0.014-ha cells, and included Bray PI, Olsen P, K, Zn, pH, organic matter, total organic C, NH-N, NOj-N, total N, mineralizable N, elevation, slope, curvature, flow accumulation, and aspect. Soybean yields consistently exhibited spatial structure. Within fields, spatial patterns of soybean yields were highly correlated across years, and we observed consistent relationships between yield and soil variables. Overall, soybean yield related positively to soil P and Zn and negatively to pH at all site-years. Models of soybean yield in relation to soil P and Zn indicate that in high pH soils at these sites, yield is optimized when soil P and Zn levels are higher than current extension recommendations. Protein and oil concentrations exhibited inconsistent spatial structure, and the spatial pattern of protein and oil concentrations differed across years. Relationships between soybean quality and soil properties were more consistent between sites within years than across years within sites, indicating that soybean quality is influenced by soil-climate interactions that function on a regional basis. [ABSTRACT FROM AUTHOR]

Many agricultural systems around the world are challenged by declining soil resources, a dry climate and increases in input costs. The cultivation of plants that are better adapted than current crop species to nutrient poor soils, a dry climate and low-input agricultural systems would aid the continued profitability and environmental sustainability of agricultural systems. This paper examines herbaceous native Australian legumes for their capacity to be developed as grain crops adapted to dry environments. The 14 genera that contain herbaceous species are Canavalia, Crotalaria, Cullen, Desmodium, Glycine, Glycyrrhiza, Hardenbergia, Indigofera, Kennedia, Lotus, Rhynchosia, Swainsona, Trigonella and Vigna. A number of these genera (e.g., Glycine, Crotalaria, Trigonella and Vigna) include already cultivated exotic grain legumes. Species were evaluated based on the extent to which their natural distribution corresponded to arid and semi-arid climatic regions, as well as the existing information on traits related to harvestability (uniformity of ripening, propensity to retain pod, pod shattering and growth habit), grain qualities (seed size, chemistry, color and the absence of toxins) and fecundity. Published data on seed yield were rare, and for many other traits information was limited. The Australian species of Vigna, Canavalia and Desmodium mainly have tropical distributions and were considered poorly suited for semi-arid temperate cropping systems. Of the remaining genera Glycyrrhiza and Crotalaria species showed many suitable traits, including an erect growth habit, a low propensity to shatter, flowers and fruits borne at the end of branches and moderate to large seeds (5 and 38 mg, respectively). The species for which sufficient information was available that were considered highest priority for further investigation were Glycine canescens, Cullen tenax, Swainsona canescens, Swainsona colutoides, Trigonella suavissima, Kennedia prorepens, Glycyrrhiza acanthocarpa, Crotalaria cunninghamii and Rhynchosia minima. [ABSTRACT FROM AUTHOR]

Multifunctional agricultural landscapes and the ecosystem services they provide are gaining more attention. One example of this is the reintroduction of native species to cool-season grassland agroecosystems managed for livestock production. While such projects have potential ecological and agronomic benefits, there is little information on how restoration management affects pasture production and quality. The objective of this study was to determine how management to establish native warm-season grasses into temperate pastures affects forage production and quality. We tested this over three years (2004 through 2006) using a field experiment with combinations of disturbance (burning and grazing), soil amendments (ambient, nitrogen, and carbon), and native grass seeding times (fall and spring). We measured aboveground net primary production (ANPP), belowground net primary production (BNPP), and two forage quality parameters—neutral detergent fiber and in vitro neutral detergent fiber digestibility. For ANPP, there was a significant disturbance × soil amendment interaction effect in each year, but the nature of the interaction varied by year. In 2005, plots where nitrogen was applied had lower BNPP than carbon and ambient plots. In 2006, burned plots had greater BNPP than grazed plots. For each level of disturbance, over 50% of the variability in the forage quality parameters was attributed to non-management variables, such as season. Our results showed no consistent effect of restoration management on pasture production or quality. They highlight the complexity of grassland agroecosystems and demonstrate the importance of evaluating the variability of treatment effects over time. [ABSTRACT FROM AUTHOR]

Greenhouse gas emissions from ruminant livestock production systems contribute significantly to the environmental footprint of agriculture. Emissions are lower for feedlot systems than for grass-based systems primarily because of the extra time required for grass-finished cattle to reach slaughter weight. In contrast, legume forages are of greater quality than grasses, which enhances intake and food conversion efficiencies, leading to improvements in production and reductions in environmental impacts compared with forage grasses. In addition, the presence of certain bioactives in legumes such as condensed tannins (CT) enhance the efficiency of energy and protein use in ruminants relative to grasses and other feeds and forages. Grazing tannin-containing legumes also reduce the incidence of bloat and improve meat quality. Synergies among nutrients and bioactives when animals graze diverse legume pastures have the potential to enhance these benefits. Thus, a diversity of legumes in feeding systems may lead to more economically, environmentally, and socially sustainable beef production than grass monocultures or feedlot rations. [ABSTRACT FROM AUTHOR]

The potential for novel forage mixtures to address reduced herbage for late season grazing was investigated. Forage legumes, sainfoin (Onobrychis viciifolia Scop.) (SF) cvs. AC Mountainview, Shoshone, and Nova (MountainSF, ShoshoneSF, and NovaSF), cicer milkvetch (Astragalus cicer L.) cv. AC Veldt (CMV), Canadian milkvetch (Astragalus canadensis L.) cv. Great Plains (CaMV), and alfalfa (Medicago sativa L.) cv. AC Yellowhead (ALF) were evaluated in binary mixtures with meadow bromegrass (Bromus riparius Rehm.) cv. Admiral (MBG), hybrid bromegrass (B. riparius × B. inermis Leyss.) cv. AC Success (HBG) and Russian wildrye [Psathyrostachys junceus (Fisch.) Nevski.] cv. Tom (RWR) for yield, botanical composition, and nutritive value on July and September harvest dates at Saskatoon and Swift Current, Saskatchewan, Canada from 2016 to 2018. Hybrid bromegrass-legume mixture produced 16–38% greater forage compared to RWR-legume (7.5 vs. 5.6 Mg ha−1 in July and 6.1 vs. 5.1 Mg ha−1 in September at Saskatoon, and 3.2 vs. 2.0 Mg ha−1 in July at Swift Current). MountainSF and ALF had the greatest legume contribution to total yield at July harvest at Swift Current (67.7 ± 3.2%) and Saskatoon (62.1 ± 2.1%), respectively, while CaMV had lowest composition at Swift Current (20.2 ± 2.5%) and Saskatoon (12.6 ± 3.5%). The CMV and ALF-grass mixtures at Saskatoon and legume-RWR mixtures at both sites in July had greatest CP content. The July harvest had greatest yield, legume content and nutritive value compared to the September harvest at both sites. Study results suggest if yield is the objective, then either ALF or CMV with HBG may be considered. If nutritive value is the goal, any legume with RWR is an option. Finally, ALF or CMV in mixture with either HBG or RWR could be summer or fall stockpile forage in the Northern Great Plains of western Canada. [ABSTRACT FROM AUTHOR]

This paper analyzes the management influence of apple growers to reduce income risk under homogenous production and market conditions. Statistical moments including mean, standard deviation, and skewness were employed to study the differences between farms referring to income variables based on full cost principle. The empirical results of 12 farms based on four-year period data of 445 orchard records suggest that farm management had a vital influence on all three statistical moments. On successful farms an increase of mean values was correlated with less negative asymmetry and significantly reduced variance of returns. High investment in preharvest labor hours was relevant for success in this perennial production system. [ABSTRACT FROM AUTHOR]

Copyright of Turkish Journal of Veterinary & Animal Sciences is the property of Scientific and Technical Research Council of Turkey and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The article discusses a consensus document that addresses compositional considerations for varieties of alfalfa and other temperate forage legumes. These components, namely, key feed nutrients, anti-nutrients and secondary plant metabolites, are described. Background material on the production, processing and uses of alfalfa and other temperate forage legumes and considerations to be taken when assessing these varieties are discussed.

Craigmill, A. L., Miller, G. R., Gehring, R., Pierce, A. N., Riviere, J. E. Meta-analysis of pharmacokinetic data of veterinary drugs using the Food Animal Residue Avoidance Databank: oxytetracycline and procaine penicillin G.J. vet. Pharmacol. Therap.27,343–353.Investigators frequently face the quandary of how to interpret the oftentimes disparate pharmacokinetic parameter values reported in the literature. Combining of data from multiple studies (meta-analysis) is a useful tool in pharmacokinetics. Few studies have explored the use of meta-analysis for veterinary species. Even fewer studies have explored the potential strengths and weaknesses of the various methods of performing a meta-analysis. Therefore, in this study we performed a meta-analysis for oxytetracycline (OTC) and procaine penicillin G (PPG) given intramuscularly to cattle. The analysis included 28 individual data sets from 18 published papers for PPG (288 data points), and 41 individual data sets from 25 published papers for OTC (489 data points). Three methods were used to calculate the parameters. The first was a simple statistical analysis of the parameter values reported in each paper. The second method was a standard Two-Stage Method (TSM) using the mean concentration vs. time data extracted from each paper. The third method was the use of nonlinear mixed effect modeling (NMEM) of the concentration vs. time data reported in the various papers, treating the mean data as if each set came from an individual animal. The results of this evaluation indicate that all three methods generate comparable mean parameter estimates for OTC and PPG. The only significant difference noted was for OTC absorption half-lives taken from the published literature, a difference attributable to the use of an alternative method of parameter calculation. The NMEM procedure offers the possibility of including covariates such as dose, age, and weight. In this study the covariates did not influence the derived parameters. A combination approach to meta-analysis of published mean data is recommended, where the TSM is the first step, followed by the NMEM approach. [ABSTRACT FROM AUTHOR]

Retained placenta, endometritis, cystic ovaries and low conception rates are often considered as interconnected conditions in cattle. Wide ranges in amounts of selenium and vitamin E supplements have been advised in late-pregnancy diets and, frequently, they can be synergistically beneficial. Animals at grass or with other green foods should be of adequate vitamin E status, but dietary selenium deficiencies are both more frequent and severe. Selenium status is important for superovulation in cattle and multiple births in ewes because of its importance in sperm transport and establishment of ova. However, the potential toxicity of selenium-enriched feed supplements restricts their use on a free-access basis. Giving sodium selenite or selenate by injection provides only a short-term response in the plasma. Barium selenate given in oil by injection provides a more durable response but has potential tissue residue problems. Compressed metallic selenium powder with iron powder in high-density reticulo-ruminal pellets gives sustained release, but uncertainties regarding possible surface coating and the variable effects of selenium particle size may require additional grinders to ensure prolonged release. Neither reticulo-ruminal pellets of such composition nor barium selenate by injection may be used worldwide because only sodium selenite and selenate have general regulatory approval. A sustained-release multi-trace element/vitamin rumen bolus system effective for several months has increased lambing percentages in ewes, and increased herd conception rates and reduced the spread of calving for herds of beef cattle. [ABSTRACT FROM AUTHOR]

This study concerned the minimum and optimum effective doses of calcium chloride needed for induction of chemosterilization in male albino rats, 30 days after a single intratesticular injection of calcium chloride (CaCl2.2H2O) solution at 2.5, 5, 10 or 20 mg per 100 g body weight per testis. There was a significant diminution in the relative wet weight of the sex organs ( p<0.01), epididymal sperm count ( p<0.001), plasma concentration of testosterone ( p<0.01), testicular activities of D,3β-hydroxy steroid dehydrogenase (D,3β-HSD), 17β-hydroxy steroid dehydrogenase (17β-HSD) ( p<0.01), glutathione S-transferase (GST) ( p<0.01), superoxide dismutase (SOD) ( p<0.01), and peroxidase ( p<0.01), significant elevations in testicular content of malondialdehyde (MDA) and conjugated dienes ( p<0.01), along with derangement of seminiferous tubular architecture and degeneration of the Leydig cells in the testis and elevations in the concentrations in the plasma of LH and FSH ( p<0.01), commencing at a dose of 5 mg, with the greatest effects at a dose of 20 mg. No significant alterations in these factors occurred at the dose of 2.5 mg in comparison to the control that received only the vehicle. There was no significant alteration in the plasma concentrations of prolactin ( p>0.05), corticosterone ( p>0.05) or fasting blood glucose or in the rectal temperature ( p>0.05) at any of the doses relative to the control group, suggesting that this chemosterilizing procedure did not exert any chronic stress on the experimental animals. From these observations, it may be suggested that 5 mg should be considered as the minimum dose, and 10 mg or 20 mg as the optimum dose, whereas 2.5 mg was ineffective for induction of chemosterilization. There would seem to be little point in using more than 20 mg of calcium chloride for this purpose. Intratesticular injection of calcium chloride at an effective dose may be considered as an alternative to surgical castration. [ABSTRACT FROM AUTHOR]

The disposition of penicillin G in piglets is described after intramuscular or subcutaneous injection of depot preparations. The piglets were injected with 33 000 IU/kg or 100 000 IU/kg benzathine + procaine penicillin G intramuscularly or subcutaneously, or 100 000 IU/kg procaine penicillin G intramuscularly or subcutaneously. Intramuscular injection of benzathine + procaine penicillin resulted in higher maximum concentration in plasma (Cmax) than did subcutaneous injection. The mean residence time (MRT) of penicillin G was longer when the drugs were injected subcutaneously rather than intramuscularly. The plasma concentration versus time profiles of the subcutaneous injections of benzathine + procaine penicillin revealed secondary peaks, possibly reflecting a certain degree of inflammation at the injection site. [ABSTRACT FROM AUTHOR]

Simple Summary: Steers and bulls exhibit clear differences and corresponding disadvantages. We evaluated that the effect of hemi-castration on the growth performance, serum testosterone levels, carcass traits, histological characteristics, and economic efficacy of Korean beef cattle (Hanwoo) to determine if hemi-castration can compensate for the disadvantages of steers and bulls. Results show that hemi-castration achieve better average daily gain and carcass weight than castration, but it lowers the marbling score and auction prices; thereby negatively affecting net income. This is due to the constant release of testosterone from one testicle. Based on the findings of this study, castration is essential to produce high-quality beef because the level of serum testosterone secreted in hemi-castration can inhibit fat development as much as in bulls. We evaluated the growth performance, serum testosterone, carcass traits, histological characteristics, and economic efficacy of castrated and hemi-castrated Korean beef cattle. Thirty-two Hanwoo calves (Initial body weight: 148.4 ± 19.8 kg) were randomly assigned into the castrated Hanwoo (CH) and hemi-castrated Hanwoo (HH) group. The experiment lasted 18 months; the animals were all slaughtered on the same day. Final body weight and average daily gain (ADG) tended to increase in the HH group compared to the CH group. Testosterone concentration was higher in HH group (5.27–14.27 ng/dL) than in the CH group (0.47–0.70 ng/dL) during the whole experimental period after castration (p < 0.05). Rib eye area was 17.08 cm2 wider in HH group than in CH group, but marbling score was improved by 3.33 in CH group compared to HH group (p < 0.01). Deposition area of adipocytes in Longissimus dorsi were higher in CH group than in HH group (p < 0.001). Net income per head was 1760 US dollar higher in the CH group than in the HH group (p < 0.04). Thus, our findings suggest that hemi-castration had positive effects on the increase in ADG and meat yield traits, with negative effects on marbling and profitability. [ABSTRACT FROM AUTHOR]

Simple Summary: The beef industry in Northern Australia is characterized by an extensive grazing system in dry tropical rangelands defined by climate change indices of very low rainfall, a prolonged dry season and feeds of low nutritive value. In response, beef cattle need to be more efficient in converting the available drought-tolerant feeds to muscle, in an attempt to minimize greenhouse gas emissions. This study addressed the problem of reducing methane emissions from tropical beef cattle with the goal of decreasing the impact of climate change and greenhouse gas emissions in Northern Australia. The primary objective was to compare the effect of supplementing tropical beef cattle with both good quality lucerne and poor quality hay with increasing levels of different Desmanthus cultivars on in vivo methane emission. The results showed that in tropical beef cattle on high-quality diets, irrespective of cultivar and emission evaluation method, Desmanthus does not reduce methane emissions. The main objective of this study was to compare the effect of supplementing beef cattle with Desmanthus virgatus cv. JCU2, D. bicornutus cv. JCU4, D. leptophyllus cv. JCU7 and lucerne on in vivo methane (CH4) emissions measured by open-circuit respiration chambers (OC) or the GreenFeed emission monitoring (GEM) system. Experiment 1 employed OC and utilized sixteen yearling Brangus steers fed a basal diet of Rhodes grass (Chloris gayana) hay in four treatments—the three Desmanthus cultivars and lucerne (Medicago sativa) at 30% dry matter intake (DMI). Polyethylene glycol (PEG) was added to the diets to neutralize tannin binding and explore the effect on CH4 emissions. Experiment 2 employed GEM and utilized forty-eight animals allocated to four treatments including a basal diet of Rhodes grass hay plus the three Desmanthus cultivars in equal proportions at 0%, 15%, 30% and 45% DMI. Lucerne was added to equilibrate crude protein content in all treatments. Experiment 1 showed no difference in CH4 emissions between the Desmanthus cultivars, between Desmanthus and lucerne or between Desmanthus and the basal diet. Experiment 2 showed an increase in CH4 emissions in the three levels containing Desmanthus. It is concluded that on high-quality diets, Desmanthus does not reduce CH4 emissions. [ABSTRACT FROM AUTHOR]

Twenty yearling male castrated Tswana goats were weighed and randomly divided into four groups of 5 animals. All the animals were fed buffel grass hay (Cenchrus ciliaris) as a basal diet, while lucerne (Medicago sativa) was fed to the control group as supplement. The other three groups were fed either Combretum apiculatum, Terminalia serecia or Euclea schimperi as a supplement. The basal diet comprised 60% of the ration, while the lucerne or the browses made up the remaining 40%. Water was provided daily. Leftover water and feed were measured daily. The goats were weighed every 2 weeks, prior to the morning feed. The data collected were subjected to analysis of variance. The average daily dry matter intake of the lucerne, C. apiculatum, T. serecia and E. schimperi supplements was 702.17, 769.91, 776.68 and 752.50 g, respectively, and was not significantly different between treatments (p>0.05). The average daily water intake was significantly different (p<0.05), with the goats fed lucerne drinking significantly more water (1270±31.62 ml) than the goats on T. serecia (1150±103.2 ml) but not significantly more than those on C. apiculatum (1166±52.25 ml) or E. schimperi (1184±100.15 ml). The average daily body weight gain was higher for the goats on lucerne at 78 g, compared to 77 g, 67 g and 64 g, for the C. apiculatum, E. schimperi and T. serecia supplements, respectively, but these were not significantly different (p>0.05). Differences in feed conversion ratio were not significant (p>0.05), but the ratio was lower for the goats on lucerne at 9.00 compared to 10.41, 11.98, and 11.23 for C. apiculatum, T. serecia and E. schimperi, respectively. The study demonstrated that these browses can be used to replace lucerne as a supplement for Tswana goats fed on buffel grass hay as the basal diet. [ABSTRACT FROM AUTHOR]

Frothy bloat is major digestive disorder of cattle grazing alfalfa pastures. Among the many factors identified to contribute to the development of frothy bloat, the disruption of rumen microbiota appears to be of central importance. Anaerobic rumen fungi (ARF) play an important role in sequential breakdown and fermentation of plant polysaccharides and promote the physical disruption of plant cell walls. In the present study, we investigated the dynamics of ARF during the development of alfalfa-induced frothy bloat and in response to bloat preventive treatments. By sequencing the internal transcribed spacer (ITS1) region of metagenomic DNA from the solid fraction of rumen contents, we were able to identify eight distinct genera of ARF, including Neocallimastix, Caecomyces, Orpinomyces, Piromyces, Cyllamyces, Anaeromyces, Buwchfawromyces, and unclassified Neocallimastigaceae. Overall, transition of steers from a baseline hay diet to alfalfa pastures was associated with drastic changes in the composition of the fungal community, but the overall composition of ARF did not differ (p > 0.05) among bloated and non-bloated steers. A correlation network analysis of the proportion of ARF and ruminal bacterial communities identified hub fungal species that were negatively correlated with several bacterial species, suggesting the presence of inter-kingdom competition among these rumen microorganisms. Interestingly, the number of negative correlations among ARF and bacteria decreased with frothy bloat, indicating a potential disruption of normal microbial profiles within a bloated rumen ecosystem. A better understanding of fungal-bacterial interactions that differ among bloated and non-bloated rumen ecosystem could advance our understanding of the etiology of frothy bloat. [ABSTRACT FROM AUTHOR]

Availability of nitrogen (N) limits pasture production. Inclusion of legumes into grass pastures can provide an alternative N source through biological N2 fixation (BNF), and enhance retention and cycling of soil C and N. Despite the use of alfalfa (Medicago sativa L.), cicer milkvetch (Astragalus cicer L.) and sainfoin (Onobrychis viciifolia Scop.) in grass-legume pastures to improve forage quality, relative BNF potentials and resource partitioning are unknown. We quantified BNF using 15N isotope dilution and estimated resource partitioning in alfalfa, two cultivars of cicer milkvetch and two cultivars of sainfoin under controlled conditions. Percentage of nitrogen derived from atmosphere followed the order alfalfa (92%) > cicer milkvetch (87%) > sainfoin (81%); corresponding to estimated N contributions of 200, 128 and 65 kg N ha−1 yr−1, respectively, based on total herbage. Root dry matter was 24% to 36% greater than shoot dry matter in all of the legumes, providing substantial below-ground C and N. Cultivars of the same species did not differ in any measured parameter (p > 0.05). Despite the lower BNF in cicer milkvetch and sainfoin compared to alfalfa, their use may not negatively affect stand productivity and C storage. [ABSTRACT FROM AUTHOR]

The growing population worldwide will create the demand for higher cereal production, in order to meet the food need of both humans and animals in the future. Consequently, the quantity of crop by-products produced by cereal cropping will increase accordingly, providing a good opportunity for fostering the development of the sustainable supply chain of renewable solid fuels and natural feedstock for animal farming. The conventional machineries used in wheat harvesting do not guarantee the possibility to collect the chaff as additional residue to the straw. The present study investigated the possibility to equip a conventional combine with a specific device, already available on the market, in order to collect the chaff either separately (onto a trailer), or together with the straw (baled). The total residual biomass increased by 0.84 t·ha−1 and 0.80 t·ha−1 respectively, without negatively affecting the performance of the combine when the chaff was discharged on the swath. Farmers can benefit economically from the extra biomass collected, although a proper sizing of the machine chain is fundamental to avoid by-product losses and lower revenue. [ABSTRACT FROM AUTHOR]

In cereal crops, chaff is a valuable lignocellulosic by-product that accounts for more than 50 Mt y−1 in Europe and is suitable for bioenergy purposes. Chaff is usually not collected due to the lack of combine harvesters that have the capability to handle it properly. The present two years experimental study investigated the hypothesis that the overall biomass collected in wheat crop can be increased by equipping the combine harvester with an aftermarket device. Chaff, discharged from the combine harvester-cleaning system, is collected by the turbine that delivers it either on the swath or on a lateral trailer towed by a tractor. The performance of all machines involved in the harvesting (combine harvester, tractor, baler, and trailer) were assessed. The chaff was collected in bales with the straw (A mode) and separately on a trailer (C mode). Comparisons with non-collected treatment (B mode) were performed in order to estimate the total chaff collected and the biomass losses. The results showed that 1.79 t FM ha−1 per year of chaff could be collected when baled with the straw, whereas 1.27 t FM ha−1 were collected separately on a trailer. Both field and material capacity were not negatively affected by the chaff collection. Therefore, our study confirmed the hypothesis that turbine technology is a valid solution for increasing the total residual biomass collected in cereal cropping for energy purposes. [ABSTRACT FROM AUTHOR]

Legumes such as alfalfa (Medicago sativa L.) and white clover (Trifolium repens L.) increase forage productivity and quality in northern temperate pastures, but require re-establishment following broadleaf weed control using herbicides. To quantify the residual effects of two herbicides (aminocyclopyrachlor and aminopyralid) on potential legume re-establishment we examined alfalfa and clover recruitment at two field sites over two years. Sites were over-seeded with alfalfa and clover to populate the seed bank, and then sprayed with herbicide, after which seedling densities were monitored in late summer and fall of the current growing season. Defoliation (via mowing) effects were also assessed to evaluate the role of vegetation competition on legume establishment. Herbicides were applied at recommended rates (1.0), and 0.5, 0.25, 0.125, 0.0625, and 0 times recommended field rates, emulating exponential herbicide degradation (one through four half-lives). Alfalfa and white clover seedling densities were negatively impacted by all rates of herbicide, with modestly greater negative impacts from aminopyralid than aminocyclopyrachlor, although responses to herbicides remained site and legume specific. Reductions in alfalfa and clover were particularly evident through the 0.25 (i.e., two half-life) herbicide rate, with reductions in alfalfa ranging from 78% to 95%, and in clover from 73% to 88%. Legume densities at the 0.125 (three half-life) rate were 39%–68% lower than those in nonsprayed control plots. Our results suggest that at least three half-lives of degradation must occur, and likely four or more, before these legumes can re-establish at densities acceptable for pasture production. These findings have implications for producers seeking to promptly re-establish forage legumes within pastures sprayed for broadleaf weed control in northern temperate regions. [ABSTRACT FROM AUTHOR]

Simple Summary: The world population is estimated to reach 9 billion people by 2050, which is estimated to increase the demand for food, fuel, and fiber by 60%. Domesticated ruminants play a vital role in this scenario because they can consume food byproducts that are nonedible for humans, contributing to livestock sustainability. Meals extracted from oilseed plants, such as soybean, canola, carinata, and camelina, are examples of food byproducts. Soybean meal is likely the byproduct most used worldwide, due to its availability and high-quality nutritional composition. However, the dependency on monocultures such as soybean is problematic due to price fluctuation and, in some countries, import dependency. Canola, camelina, and carinata meals have been investigated in the past two decades. Therefore, we aimed to summarize the results from studies in which canola, camelina, and carinata meal were fed to ruminants in order to evaluate how comparable these are to soybean meal and other common protein supplements in terms of animal digestion and performance. Based on this review, we conclude that canola meal is at least as good as soybean meal; and that camelina and carinata meal can be a valuable alternative feedstuff for livestock animals. Soybean meal (SBM) is a byproduct from the oil-industry widely used as protein supplement to ruminants worldwide due to its nutritional composition, high protein concentration, and availability. However, the dependency on monocultures such as SBM is problematic due to price fluctuation, availability and, in some countries, import dependency. In this context, oilseeds from the mustard family such as rapeseed/canola (Brassica napus and Brassica campestris), camelina (Camelina sativa), and carinata (Brassica carinata) have arisen as possible alternative protein supplements for ruminants. Therefore, the objective of this comprehensive review was to summarize results from studies in which canola meal (CM), camelina meal (CMM), and carinata meal (CRM) were fed to ruminants. This review was based on published peer-reviewed articles that were obtained based on key words that included the oilseed plant in question and words such as "ruminal fermentation and metabolism, animal performance, growth, and digestion". Byproducts from oil and biofuel industries such as CM, CMM, and CRM have been evaluated as alternative protein supplements to ruminants in the past two decades. Among the three plants reviewed herein, CM has been the most studied and results have shown an overall improvement in nitrogen utilization when animals were fed CM. Camelina meal has a comparable amino acids (AA) profile and crude protein (CP) concentration to CM. It has been reported that by replacing other protein supplements with CMM in ruminant diets, similar milk and protein yields, and average daily gain have been observed. Carinata meal has protein digestibility similar to SBM and its CP is highly degraded in the rumen. Overall, we can conclude that CM is at least as good as SBM as a protein supplement; and although studies evaluating the use of CMN and CRM for ruminants are scarce, it has been demonstrated that both oilseeds may be valuable feedstuff for livestock animals. Despite the presence of erucic acid and glucosinolates in rapeseed, no negative effect on animal performance was observed when feeding CM up to 20% and feeding CMN and CRM up to 10% of the total diet. [ABSTRACT FROM AUTHOR]

Simple Summary: The sheltering of cows in shelters is a traditional practice in India. Old, abandoned and unproductive dairy cows are housed in shelters as cow slaughter is not legally permitted in most states. The welfare assessment of these old, abandoned, infertile, unproductive and rescued cows was carried out based on the measurement of cow-based and resource-based welfare parameters. The aim of this cross-sectional study was to explore the prevalence of lameness in these sheltered cows and the risk factors associated with it. Fifty-four shelters in six states of India were visited and 1620 cows were clinically examined for lameness and measurements of other animal and resource-based welfare parameters. The prevalence of lameness in these shelters was less than that recorded in dairy cows in conventional Indian farming systems. Lameness was associated with several cow factors: inadequate body conformation, lesions on the hock and carpal joints, dirty udders, overgrown claws and diarrhea. There was evidence of an association with an absence of bedding and a steep gradient of the floor. Improvement in the feeding practices, attention towards cleanliness, and improvements in shelter flooring may reduce the prevalence of lameness and improve cow welfare in the shelters. The sheltering of old, unproductive and abandoned cows in traditional cow shelters, known as gaushalas, has been practiced in India since ancient times. Cows are kept in these shelters until they die of natural causes. The welfare of the cows in these shelters was assessed through a cross-sectional study of 54 cow shelters in six states of India. A total of 1620 cows were examined to assess the prevalence of lameness in these cows, and the associated risk factors for lameness were identified through the measurement of animal-based and resource-based welfare indicators. The overall lameness prevalence was 4.2%. The majority (86%) had mild to moderate hock joint swellings but no or only mild carpal joint injuries. Approximately one-half had mild to moderate hock joint hair loss and most were free of hock joint ulcerations. Claw overgrowth was present in almost one half of the cows. Lameness prevalence was positively correlated with coat dirtiness, hock and carpal joint lesions, diarrhea and claw overgrowth scores. In a multivariate analysis, lameness prevalence increased as the Body Condition Score (BCS) decreased and was associated with increased udder dirtiness, the ulceration of the hock joint, carpal joint injuries and claw overgrowth. Resource-based indicators measured at the shelter level suggested that an absence of bedding in the sheds and an increase in the gradient of the shed flooring increased lameness. Addressing the principle risk factors identified for lameness in the sheltered cows (low body condition, dirty udders, lesions on the hock and carpal joints, overgrown claws, and a steep floor gradient) may help to reduce this serious animal welfare problem. [ABSTRACT FROM AUTHOR]

Simple Summary: Canola meal, a by-product of oil production from canola seed, is a source of protein commonly incorporated into dairy and feedlot rations. Processing conditions and pressure treatments can alter the quality of protein in canola meal. In this study, the impact of expeller dry heat and moist heat pressure duration time on general nutritional properties, in vitro protein degradability, Maillard reaction product formation, and molecular and microscopic structural characteristics of canola meal were investigated. Increased dry heat temperature rapidly increased digestible protein and non-protein nitrogen content, and constricted amide II secondary structure. Increased moist heat pressure treatment duration promoted browning, and the conversion of protein to more intermediately and slowly degradable forms. Dry heat and moist heat pressure affected meal protein solubility and protein and lipid-related functional groups. Moist heat pressure fragmented canola meal into enzyme-resistant aggregates with crevices containing oil bodies. Induced changes may impact the supply of protein and amino acids and subsequently the yield and composition (protein and lipid) of milk produced by dairy cows. These findings benefit producers of canola meal by further describing the effects of processing and treatment conditions on protein characteristics, particularly those which affect the production potential of ruminants fed canola meal as a source of protein. To improve the protein nutritional quality of canola (Brassica napus L.) meal, further investigation of the effects of processing conditions and post-production treatments is desirable. The impact of barrel dry heat temperature (20 °C (cold press) and 100 °C (expeller)) and moist heat pressure (MHP) duration time on general nutritional properties, Maillard reaction product (MRP) formation, in vitro protein degradability, and molecular and microscopic structural characteristics of canola meals were investigated. Increased MHP duration reduced (p < 0.05) dry matter, soluble protein, rapidly degradable protein, yellowness (early MRP), whiteness (late MRPs), absorbance at 294 nm (intermediate MRPs), and amide I; and increased (p < 0.05) non-protein N, neutral detergent fibre, neutral detergent insoluble crude protein (CP), intermediately and slowly degradable protein, in vitro effective CP degradability, redness, degree of colour change, and browning. Increased dry heat temperature reduced (p < 0.01) CP and rapidly degradable protein, constricted amide II, reduced (p < 0.05) protein solubility in 0.5% KOH and increased (p < 0.05) acid-detergent fibre and intermediate MRPs. Browning index and redness exhibited potential as rapid indicators of effective CP degradability and soluble protein, respectively. Dry heat and MHP altered (p < 0.05) lipid-related functional groups. Dry heat affected napin solubility, and MHP altered cruciferin and napin solubility. Application of MHP induced the formation of proteolysis-resistant protein aggregates with crevices containing oil bodies. Induced changes may impact the supply of proteins and amino acids and subsequently the yield and composition (protein and lipid) of milk produced by dairy cows. [ABSTRACT FROM AUTHOR]