Search

Your search keyword '"Coden, Mackenzie E."' showing total 16 results
Start Over Author "Coden, Mackenzie E."
16 results on '"Coden, Mackenzie E."'

3. Metabolic Reprogramming of Nasal Airway Epithelial Cells Following Infant Respiratory Syncytial Virus Infection

Author

Connelly, Andrew R., primary, Jeong, Brian M., additional, Coden, Mackenzie E., additional, Cao, Jacob Y., additional, Chirkova, Tatiana, additional, Rosas-Salazar, Christian, additional, Cephus, Jacqueline-Yvonne, additional, Anderson, Larry J., additional, Newcomb, Dawn C., additional, Hartert, Tina V., additional, and Berdnikovs, Sergejs, additional

Published
2021
Full Text
View/download PDF

5. More than neutrophils: Lin(+)Ly6G(+)IL-5Rα(+) multipotent myeloid cells (MMCs) are dominant in normal murine bone marrow and retain capacity to differentiate into eosinophils and monocytes

Author

Jeong, Brian M, primary, Walker, Matthew T, additional, Rodriguez, Raul, additional, Coden, Mackenzie E, additional, Nagasaka, Reina, additional, Doan, Ton C, additional, Politanska, Yuliya, additional, Abdala-Valencia, Hiam, additional, and Berdnikovs, Sergejs, additional

Published
2021
Full Text
View/download PDF

10. More than neutrophils: Lin(+)Ly6G(+)IL‐5Rα(+) multipotent myeloid cells (MMCs) are dominant in normal murine bone marrow and retain capacity to differentiate into eosinophils and monocytes.

Author

Jeong, Brian M., Walker, Matthew T., Rodriguez, Raul, Coden, Mackenzie E., Nagasaka, Reina, Doan, Ton C., Politanska, Yuliya, Abdala‐Valencia, Hiam, and Berdnikovs, Sergejs

Subjects
MYELOID cells, BONE marrow, BONE marrow cells, EOSINOPHILS, NEUTROPHILS
Abstract

Bone marrow is a hematopoietic site harboring multiple populations of myeloid cells in different stages of differentiation. Murine bone marrow eosinophils are traditionally identified by Siglec‐F(+) staining using flow cytometry, whereas neutrophils are characterized by Ly6G(+) expression. However, using flow cytometry to characterize bone marrow hematopoietic cells in wild‐type mice, we found substantial gray areas in identification of these cells. Siglec‐F(+) mature eosinophil population constituted only a minority of bone marrow Lin(+)CD45(+) pool (5%). A substantial population of Siglec‐F(−) cells was double positive for neutrophil marker Ly6G and eosinophil lineage marker, IL‐5Rα. This granulocyte population with mixed neutrophil and eosinophil characteristics is typically attributable to neutrophil pool based on neutral granule staining and expression of Ly6G and myeloid peroxidase. It is distinct from Lineage(−) myeloid progenitors or Siglec‐F(+)Ly6G(+) maturing eosinophil precursors, and can be accurately identified by Lineage(+) staining and positive expression of markers IL‐5Rα and Ly6G. At 15–50% of all CD45(+) hematopoietic cells in adult mice (percentage varies by sex and age), this is a surprisingly dominant population, which increases with age in both male and female mice. RNA‐seq characterization of these cells revealed a complex immune profile and the capacity to secrete constituents of the extracellular matrix. When sorted from bone marrow, these resident cells had neutrophilic phenotype but readily acquired all characteristics of eosinophils when cultured with G‐CSF or IL‐5, including expression of Siglec‐F and granular proteins (Epx, Mbp). Surprisingly, these cells were also able to differentiate into Ly6C(+) monocytes when cultured with M‐CSF. Herein described is the discovery of an unexpected hematopoietic flexibility of a dominant population of multipotent myeloid cells, typically categorized as neutrophils, but with the previously unknown plasticity to contribute to mature pools of eosinophils and monocytes. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results