Search

Your search keyword '"Cobice, Diego F."' showing total 32 results
Start Over Author "Cobice, Diego F."
32 results on '"Cobice, Diego F."'

1. List of contributors

Author

Adams, John S., primary, Amrein, Karin, additional, Anderson, Paul H., additional, Arnold, Leggy A., additional, Arora, Juhi, additional, Artusa, Patricio, additional, Ascherio, Alberto, additional, Asmussen, Niels C., additional, Astier, Anne L., additional, Bak, Min Ji, additional, Bauerle, Kevin T., additional, Belorusova, Anna Y., additional, Benkusky, Nancy A., additional, Bernal-Mizrachi, Carlos, additional, Bhattoa, Harjit P., additional, Bikle, Daniel D., additional, Bilezikian, John P., additional, Binkley, Neil C., additional, Bischoff-Ferrari, Heike A., additional, Bishop, Charles W., additional, Blomberg Jensen, Martin, additional, Boisen, Ida Marie, additional, Boucher, Barbara J., additional, Bouillon, Roger, additional, Boyan, Barbara D., additional, Bradford, Dana, additional, Brancatella, Alessandro, additional, Buburuzan, Laura, additional, Burne, Thomas H.J., additional, Buschittari, Damien, additional, Calkins, Hannah, additional, Calvo, Mona S., additional, Camargo, Carlos A., additional, Campbell, Moray J., additional, Cantorna, Margherita T., additional, Cappellani, Daniele, additional, Carlberg, Carsten, additional, Carmeliet, Geert, additional, Cashman, Kevin D., additional, Ceglia, Lisa, additional, Cetani, Filomena, additional, Chang, Wenhan, additional, Cheadle, Charlotte, additional, Chou, Sharon H., additional, Christakos, Sylvia, additional, Christopher, Kenneth B., additional, Chu, Emily Y., additional, Chun, Rene F., additional, Cleal, Jane K., additional, Cobice, Diego F., additional, Cooper, Cyrus, additional, Coort, Susan L.M., additional, Cui, Xiaoying, additional, Curtis, Elizabeth M., additional, Danilenko, Michael, additional, Darling, Andrea L., additional, David Roodman, G., additional, Dawson-Hughes, Bess, additional, de Jongh, Renate, additional, Demay, Marie B., additional, Dennison, Elaine M., additional, Dixon, Katie M., additional, Dong, Bingning, additional, Doroudi, Maryam, additional, Dusso, Adriana, additional, Dvorzhinskiy, Aleksey, additional, Ebeling, Peter R., additional, Erben, Reinhold G., additional, Evelo, Chris T.A., additional, Eyles, Darryl, additional, Feldman, David, additional, Ferrer-Mayorga, Gemma, additional, Fleet, James C., additional, Forcellati, Marianela, additional, Foster, Brian L., additional, Gafni, Rachel I., additional, Gayan-Ramirez, Ghislaine, additional, Giovannucci, Edward, additional, Girgis, Christian M., additional, Glencross, Drew A., additional, Glorieux, Francis H., additional, Gocek, Elzbieta, additional, Goldfarb, David S., additional, Goltzman, David, additional, González-Sancho, José Manuel, additional, Grant, William B., additional, Groves, Natalie J., additional, Gysemans, Conny, additional, Harrison, Stephanie, additional, Harvey, Nicholas C., additional, Haseltine, Katherine, additional, Hawrylowicz, Catherine M., additional, Hayes, Colleen E., additional, Heckel, John E., additional, Hershberger, Pamela A., additional, Hewison, Martin, additional, Högler, Wolfgang, additional, Holick, Michael F., additional, Hollis, Bruce W., additional, Holt, Rune, additional, Hujoel, Philippe P., additional, Hyppönen, Elina, additional, Ismailova, Aiten, additional, Jablonski, Nina G., additional, Jakobsen, Jette, additional, Janssens, Wim, additional, Jeffery, Louisa, additional, Jenkinson, Carl, additional, Jensen, Marie Bagge, additional, Jetten, Anton M., additional, Jiang, Heng, additional, Johnson, Candace S., additional, Jones, Glenville, additional, Jones, Kerry S., additional, Jüppner, Harald, additional, Kalia, Vandana, additional, Kallay, Enikö, additional, Karapalis, Andrew C., additional, Kaufmann, Martin, additional, Kiely, Mairead, additional, Kim, Hanseul, additional, Kim, Tiffany Y., additional, Kojima, Hiroyuki, additional, Kooij, Ireen, additional, Kovacs, Christopher S., additional, Kremer, Richard, additional, Krieger, Kirsten, additional, Kritmetapak, Kittrawee, additional, Krueger, Diane C., additional, Kumar, Rajiv, additional, Kurihara, Noriyoshi, additional, Lane, Joseph M., additional, Lanham-New, Susan A., additional, Latic, Nejla, additional, LeBoff, Meryl S., additional, Lee, Maija B., additional, Lee, Seong Min, additional, Levine, Michael A., additional, Lewis, Richard, additional, Lewis, Rohan M., additional, Li, Wei, additional, Li, Yan Chun, additional, Lincoln, Matthew R., additional, Lips, Paul, additional, Lisse, Thomas S., additional, Liu, Eva S., additional, López de Maturana, Evangelina, additional, Lugg, Sebastian T., additional, Machado, Christopher J., additional, Maes, Karen, additional, Maestro, Miguel A., additional, Malats, Núria, additional, Malloy, Peter J., additional, Manousaki, Despoina, additional, Marcinkowska, Ewa, additional, Marcocci, Claudio, additional, Martens, Pieter-Jan, additional, Martineau, Adrian R., additional, Mason, Rebecca S., additional, Mathieu, Chantal, additional, Mayne, Phoebe, additional, McGrath, John J., additional, Mehta, Mansi, additional, Mellanby, Richard John, additional, Merchant, Nadia, additional, Meyer, Mark B., additional, Miao, Dengshun, additional, Moon, Rebecca J., additional, Mortensen, Li Juel, additional, Motlaghzadeh, Yasaman, additional, Munger, Kassandra L., additional, Muñoz, Alberto, additional, Nakamichi, Yuko, additional, Narvaez, Carmen J., additional, Nikiphorou, Elena, additional, Nonn, Larisa, additional, Pal, Lubna, additional, Parekh, Dhruv, additional, Pettifor, John M., additional, Pike, J. Wesley, additional, Pilz, Stefan, additional, Pittas, Anastassios G., additional, Pludowski, Pawel, additional, Prosser, David E., additional, Pullagura, Sri Ramulu N., additional, Raphael, Joseph, additional, Rauz, Saaeha, additional, Raza, Karim, additional, Real, Francisco X., additional, Reichrath, Jörg, additional, Richards, J. Brent, additional, Rivadeneira, Fernando, additional, Rochel, Natacha, additional, Roizen, Jeffrey D., additional, Ryan, Brittany A., additional, Sarkar, Surojit, additional, Sarmadi, Fatemeh, additional, Schafer, Anne L., additional, Schepelmann, Martin, additional, Schoenmakers, Inez, additional, Schuit, Frans, additional, Schwartz, Zvi, additional, Scott, Kayla M., additional, Sellmeyer, Deborah E., additional, Sempos, Christopher T., additional, Sepiashvili, Lusia, additional, Seshadri, Mukund, additional, Shane, Elizabeth, additional, Shaurova, Tatiana, additional, Shieh, Albert, additional, Shui, Irene, additional, Singh, Ravinder J., additional, Slominski, Andrzej T., additional, Smith, Karl W., additional, St-Arnaud, René, additional, Stein, Emily M., additional, Studzinski, George P., additional, Suda, Tatsuo, additional, Takahashi, Naoyuki, additional, Taylor, Hugh S., additional, Tebben, Peter J., additional, Thacher, Tom D., additional, Thandrayen, Kebashni, additional, Thickett, David R., additional, Tiosano, Dov, additional, Trajanoska, Katerina, additional, Tu, Chia-Ling, additional, Tuckey, Robert C., additional, Tutaworn, Teerapat, additional, Udagawa, Nobuyuki, additional, Uday, Suma, additional, Unnanuntana, Aasis, additional, van Driel, Marjolein, additional, van Leeuwen, Johannes P.T.M., additional, van Schoor, Natasja, additional, Verlinden, Lieve, additional, Vieth, Reinhold, additional, Vimaleswaran, Karina S., additional, Wagner, Carol L., additional, Wallace, Graham R., additional, Weaver, Connie M., additional, Webb, Daniel A., additional, Welsh, JoEllen, additional, White, John H., additional, Whiting, Susan J., additional, Williams, Emma L., additional, Yahyavi, Sam Kafai, additional, Yamamoto, Keiko, additional, Yates, Clayton, additional, Zagorac, Sladjana, additional, Zhang, Rong Mei, additional, Zhao, Hengguang, additional, Zhou, Ang, additional, and Zittermann, Armin, additional

Published
2024
Full Text
View/download PDF

2. Non-photopic and photopic visual cycles differentially regulate immediate, early, and late phases of cone photoreceptor-mediated vision

Author

Ward, Rebecca, Kaylor, Joanna J, Cobice, Diego F, Pepe, Dionissia A, McGarrigle, Eoghan M, Brockerhoff, Susan E, Hurley, James B, Travis, Gabriel H, and Kennedy, Breandán N

Subjects
Biomedical and Clinical Sciences, Ophthalmology and Optometry, Eye Disease and Disorders of Vision, Neurosciences, Eye, Animals, Carrier Proteins, Color Vision, Ependymoglial Cells, Fatty Acid Desaturases, Gene Deletion, Retinal Cone Photoreceptor Cells, Vitamin A, Zebrafish, cis-trans-Isomerases, vitamin A, vision, zebrafish, retina, pharmacology, chemical biology, cone-based visual behavior, Rpe65, visual cycle, Chemical Sciences, Biological Sciences, Medical and Health Sciences, Biochemistry & Molecular Biology, Biological sciences, Biomedical and clinical sciences, Chemical sciences
Abstract

Cone photoreceptors in the retina enable vision over a wide range of light intensities. However, the processes enabling cone vision in bright light (i.e. photopic vision) are not adequately understood. Chromophore regeneration of cone photopigments may require the retinal pigment epithelium (RPE) and/or retinal Müller glia. In the RPE, isomerization of all-trans-retinyl esters to 11-cis-retinol is mediated by the retinoid isomerohydrolase Rpe65. A putative alternative retinoid isomerase, dihydroceramide desaturase-1 (DES1), is expressed in RPE and Müller cells. The retinol-isomerase activities of Rpe65 and Des1 are inhibited by emixustat and fenretinide, respectively. Here, we tested the effects of these visual cycle inhibitors on immediate, early, and late phases of cone photopic vision. In zebrafish larvae raised under cyclic light conditions, fenretinide impaired late cone photopic vision, while the emixustat-treated zebrafish unexpectedly had normal vision. In contrast, emixustat-treated larvae raised under extensive dark-adaptation displayed significantly attenuated immediate photopic vision concomitant with significantly reduced 11-cis-retinaldehyde (11cRAL). Following 30 min of light, early photopic vision was recovered, despite 11cRAL levels remaining significantly reduced. Defects in immediate cone photopic vision were rescued in emixustat- or fenretinide-treated larvae following exogenous 9-cis-retinaldehyde supplementation. Genetic knockout of Des1 (degs1) or retinaldehyde-binding protein 1b (rlbp1b) did not eliminate photopic vision in zebrafish. Our findings define molecular and temporal requirements of the nonphotopic or photopic visual cycles for mediating vision in bright light.

Published
2020

8. Contribution of local regeneration of glucocorticoids to tissue steroid pools

Author

Khan, Shazia, Livingstone, Dawn E. W, Zielinska, Agnieszka, Doig, Craig L, Cobice, Diego F, Esteves, Cristina L, Man, Janet Tak Yun, Homer, Natalie Z.M., Seckl, Jonathan R, Mackay, C.Logan, Webster, Scott P, Lavery, Gareth G, Chapman, Karen E, Walker, Brian R, and Andrew, Ruth

Abstract

11β-Hydroxysteroid dehydrogenase 1 (11βHSD1) is a drug target to attenuate adverse effects of chronic glucocorticoid excess. It catalyses intracellular regeneration of active glucocorticoids in tissues including brain, liver and adipose tissue (coupled to hexose-6-phosphate dehydrogenase, H6PDH). 11βHSD1 activity in individual tissues is thought to contribute significantly to glucocorticoid levels at those sites, but its local contribution versus glucocorticoid delivery via the circulation is unknown. Here, we hypothesised that hepatic 11βHSD1 would contribute significantly to the circulating pool. This was studied in mice with Cre-mediated disruption of Hsd11b1 in liver (Alac-Cre) versus adipose tissue (aP2-Cre) or whole-body disruption of H6pdh. Regeneration of [9,12,12-2 46 H3]-cortisol (d3F) from [9,12,12-2 47 H3]-cortisone (d3E), measuring 11βHSD1 reductase activity was assessed at steady state following infusion of [9,11,12,12-2 48 H4]-cortisol (d4F) in male mice. Concentrations of steroids in plasma and amounts in liver, adipose tissue and brain were measured using mass spectrometry interfaced with matrix assisted laser desorption ionisation or liquid chromatography. Amounts of d3F were higher in liver, compared with brain and adipose tissue. Rates of appearance of d3F were ~6-fold slower in H6pdh-/- 52 mice, showing the importance of whole-body 11βHSD1 reductase activity. Disruption of liver 11βHSD1 reduced amounts of d3F in liver (by ~36%), without changes elsewhere. In contrast disruption of 11βHSD1 in adipose tissue reduced rates of appearance of circulating d3F (by ~67%) and also reduced regeneration of d3F in liver and brain (both by ~30%). Thus, the contribution of hepatic 11βHSD1 to circulating glucocorticoid levels and amounts in other tissues is less than that of adipose tissue.

Published
2023

12. Vitamin D Status and Health Outcomes in School Children in Northern Ireland: Year One Results from the D-VinCHI Study

Author

Glatt, Dominique Ulrike, primary, McSorley, Emeir, additional, Pourshahidi, L. Kirsty, additional, Revuelta Iniesta, Raquel, additional, McCluskey, Jane, additional, Beggan, Laura, additional, Slevin, Mary, additional, Gleeson, Nigel, additional, Cobice, Diego F., additional, Dobbin, Sara, additional, and Magee, Pamela J., additional

Published
2022
Full Text
View/download PDF

14. On‐tissue chemical derivatization in mass spectrometry imaging.

Author

Harkin, Carla, Smith, Karl W., Cruickshank, Faye L., Logan Mackay, C., Flinders, Bryn, Heeren, Ron M. A., Moore, Tara, Brockbank, Simon, and Cobice, Diego F.

Subjects
MASS spectrometry, IMAGING systems in chemistry, MATRIX-assisted laser desorption-ionization, DESORPTION electrospray ionization, ELECTROSPRAY ionization mass spectrometry, DERIVATIZATION, SURFACE analysis, SPATIAL resolution
Abstract

Mass spectrometry imaging (MSI) combines molecular and spatial information in a valuable tool for a wide range of applications. Matrix‐assisted laser desorption/ionization (MALDI) is at the forefront of MSI ionization due to its wide availability and increasing improvement in spatial resolution and analysis speed. However, ionization suppression, low concentrations, and endogenous and methodological interferences cause visualization problems for certain molecules. Chemical derivatization (CD) has proven a viable solution to these issues when applied in mass spectrometry platforms. Chemical tagging of target analytes with larger, precharged moieties aids ionization efficiency and removes analytes from areas of potential isobaric interferences. Here, we address the application of CD on tissue samples for MSI analysis, termed on‐tissue chemical derivatization (OTCD). MALDI MSI will remain the focus platform due to its popularity, however, alternative ionization techniques such as liquid extraction surface analysis and desorption electrospray ionization will also be recognized. OTCD reagent selection, application, and optimization methods will be discussed in detail. MSI with OTCD is a powerful tool to study the spatial distribution of poorly ionizable molecules within tissues. Most importantly, the use of OTCD−MSI facilitates the analysis of previously inaccessible biologically relevant molecules through the adaptation of existing CD methods. Though further experimental optimization steps are necessary, the benefits of this technique are extensive. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

15. Vitamin D and Bone Health of Older Adults within Care Homes: An Observational Study.

Author

Feehan, Orlagh, Armstrong, David J., Magee, Pamela J., Pourshahidi, Kirsty L., Strain, J. J., Beggan, Laura, Cobice, Diego F., and McSorley, Emeir M.

Abstract

Limited studies have reported vitamin D status and health outcomes in care home residents, a group at risk of vitamin D deficiency. This study investigated serum 25-hydroxyvitamin D (25-OHD) concentrations in older adults within care homes in Northern Ireland (NI) and its association with musculoskeletal health (ultrasound T-score, muscle strength, Timed Up & Go test (TUG)), bone turnover markers (BTMs), and immune function markers. A total of 87 participants were recruited with mean ± SD age 83.2 ± 7.9 years. Mean ± SD serum 25-OHD concentration (n 69) was 49.52 ± 35.58 nmol/L. Vitamin D deficiency (25-OHD <25 nmol/L) was observed in 34.8% (n 24) of participants with 17.4% (n 12) classified as insufficient (25-OHD 25–50 nmol/L) and 47.8% (n 33) as sufficient (25-OHD >50 nmol/L). 25-OHD concentration was not an independent predictor of T-score, muscle strength, TUG, or inflammatory cytokines. After adjusting for covariates, a significant negative association was observed between 25-OHD concentration and the BTMs; osteocalcin (β = −0.395; p = 0.001), procollagen type 1 N propeptide (P1NP) (β = −0.320; p = 0.012), and C-terminal telopeptide of type 1 collagen (CTX) (β = −0.377; p = 0.003). Higher 25-OHD concentration was positively associated with use of vitamin D ± calcium supplementation (β = 0.610; p < 0.001). Vitamin D deficiency and insufficiency were highly prevalent in this sample of care home residents in NI. Higher 25-OHD concentration was associated with greater supplement use and with reduced bone turnover, which in this population is linked with reduced bone loss. These findings emphasize the need for a mandatory vitamin D ± calcium supplementation policy specific for care home residents. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

16. Maternal and child fatty acid desaturase genotype as determinants of cord blood long-chain PUFA (LCPUFA) concentrations in the Seychelles Child Development Study

Author

Conway, Marie C., primary, McSorley, Emeir M., additional, Mulhern, Maria S., additional, Spence, Toni, additional, Weslowska, Maria, additional, Strain, J. J., additional, van Wijngaarden, Edwin, additional, Davidson, Phil W., additional, Myers, Gary J., additional, Wahlberg, Karin E., additional, Shamlaye, Conrad F., additional, Cobice, Diego F., additional, Hyland, Barry W., additional, Pineda, Daniela, additional, Broberg, Karin, additional, and Yeates, Alison J., additional

Published
2021
Full Text
View/download PDF

19. List of contributors

Author

Adams, John S., Amrein, Karin, Anderson, Paul H., Arnold, Leggy A., Arora, Juhi, Artusa, Patricio, Ascherio, Alberto, Asmussen, Niels C., Astier, Anne L., Bak, Min Ji, Bauerle, Kevin T., Belorusova, Anna Y., Benkusky, Nancy A., Bernal-Mizrachi, Carlos, Bhattoa, Harjit P., Bikle, Daniel D., Bilezikian, John P., Binkley, Neil C., Bischoff-Ferrari, Heike A., Bishop, Charles W., Blomberg Jensen, Martin, Boisen, Ida Marie, Boucher, Barbara J., Bouillon, Roger, Boyan, Barbara D., Bradford, Dana, Brancatella, Alessandro, Buburuzan, Laura, Burne, Thomas H.J., Buschittari, Damien, Calkins, Hannah, Calvo, Mona S., Camargo, Carlos A., Jr., Campbell, Moray J., Cantorna, Margherita T., Cappellani, Daniele, Carlberg, Carsten, Carmeliet, Geert, Cashman, Kevin D., Ceglia, Lisa, Cetani, Filomena, Chang, Wenhan, Cheadle, Charlotte, Chou, Sharon H., Christakos, Sylvia, Christopher, Kenneth B., Chu, Emily Y., Chun, Rene F., Cleal, Jane K., Cobice, Diego F., Cooper, Cyrus, Coort, Susan L.M., Cui, Xiaoying, Curtis, Elizabeth M., Danilenko, Michael, Darling, Andrea L., David Roodman, G., Dawson-Hughes, Bess, de Jongh, Renate, Demay, Marie B., Dennison, Elaine M., Dixon, Katie M., Dong, Bingning, Doroudi, Maryam, Dusso, Adriana, Dvorzhinskiy, Aleksey, Ebeling, Peter R., Erben, Reinhold G., Evelo, Chris T.A., Eyles, Darryl, Feldman, David, Ferrer-Mayorga, Gemma, Fleet, James C., Forcellati, Marianela, Foster, Brian L., Gafni, Rachel I., Gayan-Ramirez, Ghislaine, Giovannucci, Edward, Girgis, Christian M., Glencross, Drew A., Glorieux, Francis H., Gocek, Elzbieta, Goldfarb, David S., Goltzman, David, González-Sancho, José Manuel, Grant, William B., Groves, Natalie J., Gysemans, Conny, Harrison, Stephanie, Harvey, Nicholas C., Haseltine, Katherine, Hawrylowicz, Catherine M., Hayes, Colleen E., Heckel, John E., Hershberger, Pamela A., Hewison, Martin, Högler, Wolfgang, Holick, Michael F., Hollis, Bruce W., Holt, Rune, Hujoel, Philippe P., Hyppönen, Elina, Ismailova, Aiten, Jablonski, Nina G., Jakobsen, Jette, Janssens, Wim, Jeffery, Louisa, Jenkinson, Carl, Jensen, Marie Bagge, Jetten, Anton M., Jiang, Heng, Johnson, Candace S., Jones, Glenville, Jones, Kerry S., Jüppner, Harald, Kalia, Vandana, Kallay, Enikö, Karapalis, Andrew C., Kaufmann, Martin, Kiely, Mairead, Kim, Hanseul, Kim, Tiffany Y., Kojima, Hiroyuki, Kooij, Ireen, Kovacs, Christopher S., Kremer, Richard, Krieger, Kirsten, Kritmetapak, Kittrawee, Krueger, Diane C., Kumar, Rajiv, Kurihara, Noriyoshi, Lane, Joseph M., Lanham-New, Susan A., Latic, Nejla, LeBoff, Meryl S., Lee, Maija B., Lee, Seong Min, Levine, Michael A., Lewis, Richard, Lewis, Rohan M., Li, Wei, Li, Yan Chun, Lincoln, Matthew R., Lips, Paul, Lisse, Thomas S., Liu, Eva S., López de Maturana, Evangelina, Lugg, Sebastian T., Machado, Christopher J., Maes, Karen, Maestro, Miguel A., Malats, Núria, Malloy, Peter J., Manousaki, Despoina, Marcinkowska, Ewa, Marcocci, Claudio, Martens, Pieter-Jan, Martineau, Adrian R., Mason, Rebecca S., Mathieu, Chantal, Mayne, Phoebe, McGrath, John J., Mehta, Mansi, Mellanby, Richard John, Merchant, Nadia, Meyer, Mark B., Miao, Dengshun, Moon, Rebecca J., Mortensen, Li Juel, Motlaghzadeh, Yasaman, Munger, Kassandra L., Muñoz, Alberto, Nakamichi, Yuko, Narvaez, Carmen J., Nikiphorou, Elena, Nonn, Larisa, Pal, Lubna, Parekh, Dhruv, Pettifor, John M., Pike, J. Wesley, Pilz, Stefan, Pittas, Anastassios G., Pludowski, Pawel, Prosser, David E., Pullagura, Sri Ramulu N., Raphael, Joseph, Rauz, Saaeha, Raza, Karim, Real, Francisco X., Reichrath, Jörg, Richards, J. Brent, Rivadeneira, Fernando, Rochel, Natacha, Roizen, Jeffrey D., Ryan, Brittany A., Sarkar, Surojit, Sarmadi, Fatemeh, Schafer, Anne L., Schepelmann, Martin, Schoenmakers, Inez, Schuit, Frans, Schwartz, Zvi, Scott, Kayla M., Sellmeyer, Deborah E., Sempos, Christopher T., Sepiashvili, Lusia, Seshadri, Mukund, Shane, Elizabeth, Shaurova, Tatiana, Shieh, Albert, Shui, Irene, Singh, Ravinder J., Slominski, Andrzej T., Smith, Karl W., St-Arnaud, René, Stein, Emily M., Studzinski, George P., Suda, Tatsuo, Takahashi, Naoyuki, Taylor, Hugh S., Tebben, Peter J., Thacher, Tom D., Thandrayen, Kebashni, Thickett, David R., Tiosano, Dov, Trajanoska, Katerina, Tu, Chia-Ling, Tuckey, Robert C., Tutaworn, Teerapat, Udagawa, Nobuyuki, Uday, Suma, Unnanuntana, Aasis, van Driel, Marjolein, van Leeuwen, Johannes P.T.M., van Schoor, Natasja, Verlinden, Lieve, Vieth, Reinhold, Vimaleswaran, Karina S., Wagner, Carol L., Wallace, Graham R., Weaver, Connie M., Webb, Daniel A., Welsh, JoEllen, White, John H., Whiting, Susan J., Williams, Emma L., Yahyavi, Sam Kafai, Yamamoto, Keiko, Yates, Clayton, Zagorac, Sladjana, Zhang, Rong Mei, Zhao, Hengguang, Zhou, Ang, and Zittermann, Armin

Published
2023
Full Text
View/download PDF

20. The influence of fish consumption on serum n-3 polyunsaturated fatty acid (PUFA) concentrations in women of childbearing age: a randomised controlled trial (the iFish Study)

Author

Conway, Marie C., primary, McSorley, Emeir M., additional, Mulhern, Maria S., additional, Spence, Toni, additional, Wijngaarden, Edwin van, additional, Watson, Gene E., additional, Wahlberg, Karin, additional, Pineda, Daniela, additional, Broberg, Karin, additional, Hyland, Barry W., additional, Cobice, Diego F., additional, Strain, J. J., additional, and Yeates, Alison J., additional

Published
2020
Full Text
View/download PDF

27. Spatial Localization and Quantitation of Androgens in Mouse Testis by Mass Spectrometry Imaging

Author

Cobice, Diego F, Livingstone, Dawn E. W., Mackay, Colin Logan, Goodwin, Richard J. A., Smith, Lee B., Walker, Brian R., and Andrew, Ruth

Abstract

Androgens are essential for male development and reproductive function. They are transported to their site of action as blood-borne endocrine hormones, but can also be produced within tissues to act in intracrine and paracrine fashions. Because of this, circulating concentrations may not accurately reflect the androgenic influence within specific tissue microenvironments. Mass spectrometry imaging permits regional analysis of small molecular species directly from tissue surfaces. However, due to poor ionization and localized ion suppression, steroid hormones are difficult to detect. Here derivatization with Girard T reagent was used to charge-tag testosterone and 5α-dihydrotestosterone allowing direct detection of these steroids in mouse testes, in both basal and maximally-stimulated states and in rat prostate. Limits of detection were ~0.1 pg for testosterone. Exemplary detection of endogenous steroids was achieved by matrix-assisted laser desorption ionization and either Fourier transform ion cyclotron resonance detection (at 150 μm spatial resolution) or quadrupole-time of flight detection (at 50 μm spatial resolution). Structural confirmation was achieved by collision induced fragmentation following liquid extraction surface desorption and electrospray ionization. This application broadens the scope for derivatization strategies on tissue surfaces to elucidate local endocrine signaling in health and disease

Published
2016

31. Derivatization of estrogens enhances specificity and sensitivity of analysis of human plasma and serum by liquid chromatography tandem mass spectrometry

Author

Faqehi, Abdullah M.M., Cobice, Diego F., Naredo, Gregorio, Mak, Tracy C.S., Upreti, Rita, Gibb, Fraser W., Beckett, Geoffrey J., Walker, Brian R., Homer, Natalie Z.M., and Andrew, Ruth

Subjects
17αE2, 17α-estradiol, Male, LC-MS, liquid chromatography-mass spectrometry, Chemistry(all), cps, counts per second, IS, internal standards, CID, collision-induced dissociation, RSD, relative standard deviation, Pyridinium Compounds, E2, estradiol, LODs, limits of detection, Tandem Mass Spectrometry, TEA, triethylamine, 3,4-[13C2E1, 3,4-[13C]2-estrone, FA, formic acid, FMP-TS, 2-fluoro-1-methylpyridinium-p-toluenesulfonate, Aged, 80 and over, LOQs, limits of quantitation, ESI, electrospray ionization, UHPLC, ultra high performance liquid chromatography, Estradiol, APCI, atmospheric pressure chemical ionization, Benzenesulfonates, Solid Phase Extraction, d4E1, 2,4,16,16-[2H]4-estrone, MD, mean difference, Middle Aged, E1, estrone, Derivatization, MRM, multiple reactions monitoring, Postmenopause, SPE, solid phase extraction, HPLC, high performance liquid chromatography, Female, d4E2, 2,4,16,16-[2H]4-estradiol, Adult, Spectrometry, Mass, Electrospray Ionization, Estrone, Liquid chromatography, Article, Young Adult, Humans, RME, relative mean error, Aged, D, difference, Mass spectrometry, LC-MS/MS, liquid chromatography tandem mass spectrometry, SNR, signal/noise, Reproducibility of Results, Estrogens, 13C2E2, 3,4-[13C]2-estradiol, Premenopause, Methylpyridinium ether, SD, standard deviation, Chromatography, Liquid
Abstract

Estrogens circulate at concentrations less than 20 pg/mL in men and postmenopausal women, presenting analytical challenges. Quantitation by immunoassay is unreliable at these low concentrations. Liquid chromatography tandem mass spectrometry (LC–MS/MS) offers greater specificity and sometimes greater sensitivity, but ionization of estrogens is inefficient. Introduction of charged moieties may enhance ionization, but many such derivatives of estrogens generate non-specific product ions originating from the “reagent” group. Therefore an approach generating derivatives with product ions specific to individual estrogens was sought. Estrogens were extracted from human plasma and serum using solid phase extraction and derivatized using 2-fluoro-1-methylpyridinium-p-toluenesulfonate (FMP-TS). Electrospray in positive mode with multiple reaction monitoring using a QTrap 5500 mass spectrometer was used to quantify “FMP” derivatives of estrogens, following LC separation. Transitions for the FMP derivatives of estrone (E1) and estradiol (E2) were compound specific (m/z 362→238 and m/z 364→128, respectively). The limits of detection and quantitation were 0.2 pg on-column and the method was linear from 1–400 pg/sample. Measures of intra- and inter-assay variability, precision and accuracy were acceptable (, Graphical abstract fx1, Highlights • Quantitative analysis of low amounts of estrone and estradiol in plasma and serum. • Quantitation across physiological range in men and pre- and post-menopausal women. • Methylpyridinium ether derivatives improve analytical specificity and sensitivity.

Full Text
View/download PDF

32. Spatial detection and consequences of nonrenal calcitriol production as assessed by targeted mass spectrometry imaging.

Author

Meyer MB, Lee SM, Cichanski SR, Cobice DF, and Pike JW

Subjects
Animals, Mice, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods, Male, Cholecalciferol metabolism, Inflammation metabolism, Female, Calcitriol metabolism, 25-Hydroxyvitamin D3 1-alpha-Hydroxylase metabolism, 25-Hydroxyvitamin D3 1-alpha-Hydroxylase genetics, Mice, Knockout, Kidney metabolism
Abstract

The immune benefits of vitamin D3 supplementation beyond calcium and phosphate maintenance are highly clinically debated. Kidney expression of CYP27B1 is the source of endocrine, circulating 1,25(OH)2D3 (active form of vitamin D) that maintains serum calcium and phosphate. 1,25(OH)2D3 may also be made by the CYP27B1 enzyme in nonrenal cells, like immune cells, in a process driven by cellular availability of 25(OH)D3 and inflammation. Due to the endocrine nature of 1,25(OH)2D3 in circulation, it is difficult to discern between these 2 sources. We recently created a regulatory deletion model of Cyp27b1 (M1/M21-DIKO) where mice have normal inflammatory-regulated Cyp27b1 expression in nonrenal tissues (unlike global Cyp27b1-KO) but no expression within the kidney. Here, utilizing on-tissue chemical derivatization and matrix assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI), we investigated the distribution of 1,25(OH)2D3 and 25(OH)D3 in the kidney, liver, spleen, and thymus. MALDI-MSI demonstrated increased 1,25(OH)2D3 in nonrenal tissues such as the spleen after vitamin D3 supplementation in M1/M21-DIKO mice. Additionally, from this, we found increased Il4 and decreased Tnfa in the spleen after vitamin D3 supplementation. Taken together, these data demonstrate nonrenal production of 1,25(OH)2D3 in vivo and provide a consequence of vitamin D3 supplementation and nonrenal 1,25(OH)2D3 production in cytokine changes.

Published
2024
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results