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We are developing a new IR imaging technique, enhanced thermal imaging, that can delineate blood vessels embedded in water-rich tissue in real time. The technique is a combination of thermal IR imaging (8-10 μm) and selective heating of blood (∼0.5°C) relative to surrounding water-rich tissue using LED sources (530 nm) at low powers (irradiance

International audience; It is shown that the resolution of virtual images of dye-doped dielectric nanospheres obtained through dielectric microspheres can be increased beyond the classical diffraction limit by decreasing the period of nanoplasmonic array used for localized plasmonic structured illumination of these objects. In contrast to far-field superlens design, the superresolution is obtained without using metallic layers and gratings after superlens. In addition, it is shown that the postimaging processing, which represents an intrinsic part of SIM, is not required for achieving the super-resolved images. This observation is interpreted based on a concept that the radiation of objects placed at the surface of nanoplasmonic arrays with sufficiently short periods is initially provided into folded dispersions of nanoplamonic array, so that the diffraction orders responsible for super-resolution are more efficiently coupled to dielectric microspherical antenna compared to that for the propagating modes within the cutoff frequency.

A classification of label-free super-resolution imaging mechanisms is given based on the nonlinear reduction of the point-spread function (PSF), near-field scanning, image magnification and gain, structured and sparse illumination, and information approaches. We argue that the super-resolution capability of contact microspheres stems from an image magnification effect taking place in close proximity to the object with contributions of its optical near-fields. We discuss several conditions for quantifying the super-resolution in a label-free microscopy: i) use of standalone objects or long-period arrays as opposed to subwavelength periodic structures, ii) use a convolution with two-dimensional PSF for calculating images, and iii) avoidance of coherent imaging which can lead to dramatic artifacts. We demonstrate a resolution of ∼λ/7 for imaging nanoplasmonic structures and propose a combination of microspherical nanoscopy with nanoplasmonic illumination for imaging biomedical samples. We applied these techniques for imaging actin protein filaments and yeast cells and observed a resolution advantage over standard microscopy.

Microspherical Nanoscopy uses a microsphere as a contact lens which allows for resolving nanometer sized objects using visible light. Nanoplasmonic structures have been used in combination with microspherical nanoscopy to achieve resolutions beyond the classical diffraction limit. The mechanisms of such super-resolution imaging can include the optical near field coupling and the virtual image magnification effects. In this work, we quantify the resolution of microspherical nanoscopy using a basic fluorescence microscope equipped with a standard 40× (NA = 0.6) objective which can be found in most of the labs performing histology or microscopy analyses in a clinical environment. We perform the resolution quantification in three distinct structures: (1) F-Actin proteins on a microscope slide, (2) F-Actin proteins imaged through a high index microsphere on a microscope slide, and (3) F-Actin proteins imaged through a high index microsphere on a nanoplasmonic array. The high index microspheres (n∼2) are embedded in an elastomer slab. Using a microscope we achieve a resolution of (1) ∼λ/0.7, (2) − λ/2.5, and (3) ∼λ/2.8 for each situation.

International audience; The resolution of microsphere-based nanoscopy is studied using fluorescently labeled nanospheres and F-actin protein filaments with the emission coupled to the localized surface plasmon resonances in the underlying Au nanodisk arrays. Virtual imaging is performed through high-index microspheres embedded in plastic coverslips placed in contact with the nanoscale objects. For 150 and 200 nm periods of nanoplasmonic arrays, the imaging has a solid immersion lens-limited resolution, whereas for shorter periods of 80 and 100 nm, the resolution was found to increase up to ∼λ/7, where λ is the emission wavelength. The results cannot be interpreted within a framework of a regular localized plasmonic structured illumination microscopy since the array period was significantly shorter than the wavelength and postimaging processing was not used. It is hypothesized that the observed super-resolution is based on coupling of the emission of nanoscale objects to strongly localized near-field maxima in the adjacent plasmonic metasurfaces followed by evanescent coupling to high-index microspheres.