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Kunzea ambigua is a small shrub belonging to the Myrtaceae family and the leaves are steam-distilled to produce a therapeutically active essential oil. With production moving from wild-harvested to orchardised stands, there is a need for harvest management of kunzea oil. This study compared the regrowth, essential oil content and composition of kunzea plants after harvesting vegetative material to a depth of 0.2 m above ground level (shallow-cut), relative to plants cut to a depth of 0.1 m above ground level (deep-cut) over the 2018/2019 growing season. Increased vegetative biomass accounted for the increased oil yield and was caused by consistently higher growth rates of 50 to 60% across all seasons in shallow-cut crops relative to those subject to deep-cut. Total soluble sugar concentrations were higher in the leaves and lower in the roots of deep-cut treated plants compared to the other treatments, indicating defoliated K. ambigua responds by mobilising sugars into above-ground biomass. The overall essential oil content of leaves was constant regardless of season, though the oil yield for shallow-cut was 1.9-fold higher at 11.79 ± 0.23 g/m2 compared to deep-cut (6.24 ± 0.18 g/m2). An interactive effect of harvest intensity with season was recorded for all major components except for a non-significant effect of season on terpinen-4-ol. Bicyclogermacrene and α-pinene were elevated in both shallow- and deep-cut treatments relative to control (un-cut) in spring, possibly due to the plant defense response after de-foliation. The highest percentage of bioactive compounds (1,8-cineole and viridiflorol) were present in autumn. Therefore, the recovery of biomass post-harvest is optimised by shallow-cut harvests, and the profile of kunzea oil can be manipulated to elevate levels of specific bioactive components by selecting to crop in autumn/spring. [ABSTRACT FROM AUTHOR]

Protected cropping systems (PCS) de-risk adverse climatic effects in intensive horticultural production but alter the growing environment. The objectives of this study were to investigate the effects of modern, commercial-scale PCS on sweet cherry orchard microclimate, tree water uptake and fruit quality. Sap flow sensors and weather stations were positioned at four locations under a 21 ha PCS at varying elevations (125, 114, 111, 102 m above sea level) and distances from the block boundary (105, 75, 60 or 50 m, referred to hereafter as Locations 1 to 4, respectively). Generalised additive models (GAMs) were used to predict the effect of individual climate parameters (temperature, relative humidity, solar radiation and wind speed) on tree sap flow at each of the four locations. Average and maximum temperatures and average minimum relative humidity (RH) were higher (15.9°C, 26.1°C and 49.0%) at locations with higher elevations and located further from the PCS boundary (locations 1 and 2) in contrast to locations at lower elevations and closer proximity to the PCS boundary (locations 3 and 4) (15.4°C, 24.6°C and 48.1%). Predicted sap flow was strongly correlated (r² = 0.92) with time across the four locations under the PCS. GAMS modelling indicated that the hourly water uptake by trees within close proximity to the block boundary (locations 3 and 4) responded with greater intensity to increases in temperature and reductions in relative humidity, taking up on average 0.15 L h-1 (at temperatures >30°C) and 0.08 L h-1 (at RH<50%), respectively, in contrast to trees further under the PCS (locations 1 and 2) where average tree water uptake was 0.08 and 0.04 L h-1 at temperatures >30°C and RH<50%, respectively. Highest average predicted hourly tree sap flow was associated with high wind speeds (0.67 L h-1) and low relative humidity levels (0.61 L h-1). Fruit harvested from locations further from the PCS boundary had significantly higher dry matter content (18.2%), total soluble solids (17.8%) and compression firmness (311.3 g mm-1) in contrast to fruit closer to the PCS boundaries (16.1%, 15.7% and 258.3 g mm-1). This study provides greater understanding of the effects of PCS on microclimate and consequences for tree water uptake and fruit quality. [ABSTRACT FROM AUTHOR]

Developing systems for the commercial production of native plant species is required to ensure a sustainable supply of quality material. The aim of this study was to examine the influence of key nutrients on the early establishment ofTasmannia lanceolatafor potential plantation production of leaves from which polygodial is extracted. Treatments of N, P and K fertilizers were imposed on a single clone in a pot trial with a factorial design. Levels of all other essential nutrients were kept constant. The highest application level of N (20 mM, 2.32% N in leaves) achieved the highest level of polygodial as a percentage of dry matter. The highest P (2 mM, 0.42% P in leaves) and K (12 mM, 1.73% K in leaves) treatments produced a noticeable decline in total polygodial yield, with medium-level treatments of both nutrients producing the highest levels of polygodial. The implications of these findings for the production of commercial quantities ofT. lanceolataleaf material are discussed. [ABSTRACT FROM AUTHOR]