1. Effect of the Application of PGF 2α Associated With Ovulation Induction in a Fixed-Time Superovulation Programme for Precocious Nellore Heifers.
- Author
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Sanches TK, Soares PMC, Marques MO, da Silva RCP, Pfeifer LFM, Zangirolamo AF, Morotti F, and Seneda MM
- Subjects
- Animals, Cattle, Female, Pregnancy, Cloprostenol pharmacology, Cloprostenol administration & dosage, Buserelin pharmacology, Buserelin administration & dosage, Follicle Stimulating Hormone pharmacology, Follicle Stimulating Hormone administration & dosage, Dinoprost pharmacology, Dinoprost administration & dosage, Gonadotropin-Releasing Hormone pharmacology, Gonadotropin-Releasing Hormone administration & dosage, Superovulation drug effects, Insemination, Artificial veterinary, Ovulation Induction veterinary, Ovulation Induction methods, Estradiol pharmacology, Estradiol administration & dosage, Estradiol analogs & derivatives, Progesterone pharmacology, Progesterone administration & dosage
- Abstract
This study evaluated the effect of prostaglandin F2α (PGF2α) associated with gonadotropin-releasing hormone (GnRH) for ovulation induction in precocious indicus heifers submitted to a fixed-time superovulation (SOV) programme. Precocious Nellore heifers (n = 35), aged 13 months, were subjected to the SOV protocol. On day 0 (D0), all animals received intravaginal insertion of a progesterone (P4) device along with intramuscular administration of 2 mg of oestradiol benzoate, plus 200 IU of follicle-stimulating hormone in decreasing doses, with 12-h intervals between D4 and D7, in addition to 150 μg of D-cloprostenol on D6 and device removal on D7. On D8, the donors received 10.5 μg of buserelin acetate and the treatment group received 300 μg of D-cloprostenol/PGF
2α . Artificial insemination was performed 12 h and 24 h after GnRH administration using frozen semen. On D15 of the protocol (i.e., D7 after insemination), the embryos were collected and evaluated. All animals passed through the control and treatment groups. Results were evaluated by analysis of variance using an adjusted mixed-effects model (p < 0.05). There was no difference in the total number of embryos between the control and treatment groups (10.40 ± 1.52 vs. 9.60 ± 1.36; p = 0.63) or viable embryos (6.30 ± 1.22 vs. 4.30 ± 0.71). For precocious indicus heifers, treatment with PGF2α in association with GnRH did not affect embryo production in the fixed-time SOV protocol., (© 2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.)- Published
- 2024
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