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31 results on '"Cloarec, J.-P."'

7. Sensing performances of integrated ISFETs on 28 nm CMOS FDSOI technology

Author

Souifi, A, Ayele G, T, Cloarec J, P, Boeuf, F, Ecoffey, S, Drouin, D, Monfray, S, LN2, UMI3463, Laboratoire Nanotechnologies et Nanosystèmes [Sherbrooke] (LN2), Université de Sherbrooke (UdeS)-École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Institut Interdisciplinaire d'Innovation Technologique [Sherbrooke] (3IT), and Université de Sherbrooke (UdeS)

Subjects
[SPI]Engineering Sciences [physics], [SPI] Engineering Sciences [physics], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020

8. Innovative Gold Nanostructred biochips for surface plasmon resonance imaging and surface enhanced raman spectroscopy

Author

Bryche, J-F., Moreau, Julien, Barbillon, G., Sarkar, M., Maillart, E., Banville, F., Charette, P. G, Lamy de La Chapelle, Marc, Cloarec, J-P., Bartenlian, B., Canva., M., LN2, UMI3463, Laboratoire Nanotechnologies Nanosystèmes (LN2 ), Université de Sherbrooke (UdeS)-École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut Interdisciplinaire d'Innovation Technologique [Sherbrooke] (3IT), Université de Sherbrooke (UdeS), Laboratoire Charles Fabry / Biophotonique, Laboratoire Charles Fabry (LCF), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS), Service de Neurologie [CHU Pitié-Salpêtrière], IFR70-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut d'électronique fondamentale (IEF), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Sherbrooke (UdeS)-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Université Paris-Sud - Paris 11 (UP11)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)

Subjects
[SPI]Engineering Sciences [physics], [SPI] Engineering Sciences [physics], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

9. Ultrasensitive CMOS Electro-chemical Sensor Based on Fully Depleted SOI MOSFET

Author

Ayele G, T, Souifi, A., Cloarec J, P, Monfray, S., Boeuf, F., Ecoffey, S., Drouin, D., LN2, UMI3463, Laboratoire Nanotechnologies Nanosystèmes (LN2 ), Université de Sherbrooke (UdeS)-École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut Interdisciplinaire d'Innovation Technologique [Sherbrooke] (3IT), Université de Sherbrooke (UdeS), Institut National des Sciences Appliquées (INSA), STMicroelectronics [Crolles] (ST-CROLLES), and Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])

Subjects
[SPI]Engineering Sciences [physics], [SPI] Engineering Sciences [physics], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

10. Ultrasensitive Extended-Gate Ion-Sensitive-Field-Effect-Transistor Developed Utilizing an Industrial UTBB FDSOI Platform

Author

Ayele, G. T., Monfray, S., Boeuf, F., Cloarec, J.-P., Ecoffey, S., Drouin, D., Souifi, E. Puyoo And A., Laboratoire Nanotechnologies Nanosystèmes (LN2 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Sherbrooke (UdeS)-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Centre National de la Recherche Scientifique (CNRS), Institut Interdisciplinaire d'Innovation Technologique [Sherbrooke] (3IT), Université de Sherbrooke (UdeS), STMicroelectronics [Crolles] (ST-CROLLES), INL - Chimie et Nanobiotechnologies (INL - C&N), Institut des Nanotechnologies de Lyon (INL), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-École Centrale de Lyon (ECL), Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE), Université de Sherbrooke (UdeS)-École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-École Centrale de Lyon (ECL), and LN2, UMI3463

Subjects
[SPI]Engineering Sciences [physics], [SPI] Engineering Sciences [physics], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

12. Collective self-assembly of large sets of nanoparticles on nanopatterned functionalized substrates

Author

Palazon, Francisco, Ansanay-Alex, Salomé, Rojo Romeo, Pedro, Chevalier, Céline, Gehin, Thomas, Belarouci, Ali, Laurenceau, Emmanuelle, Le Mogne, Thierry, Léonard, Didier, Bryche, Jean-François, Barbillon, Gregory, Bartenlian, Bernard, Olivéro, Aurore, Sarkar, Mitradeep, Besbes, Mondher, Maillart, Emmanuel, Moreau, Julien, Canva, Michael, Chevolot, Yann, Cloarec, .J.-P., INL - Chimie et Nanobiotechnologies (INL - C&N), Institut des Nanotechnologies de Lyon (INL), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-École Centrale de Lyon (ECL), Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE), INL - Nanophotonique (INL - Photonique), INL - Photovoltaïque (INL - PV), Laboratoire de Tribologie et Dynamique des Systèmes (LTDS), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Ecole Nationale d'Ingénieurs de Saint Etienne-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut d'électronique fondamentale (IEF), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Charles Fabry / Biophotonique, Laboratoire Charles Fabry (LCF), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS), and HORIBA Scientific [France]

Subjects
[CHIM.MATE]Chemical Sciences/Material chemistry, [CHIM.OTHE]Chemical Sciences/Other, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

13. Combining Surface Nanostructuration and Surface Chemistry at the Submicronic Scale: Towards a Bimodal SPRI / SERS Biosensor

Author

Cloarec, .J.-P., Ansanay-Alex, Salomé, Chevolot, Yann, Laurenceau, Emmanuelle, Palazon, Francisco, Bryche, J-F., Barbillon, Grégory, Bartenlian, Bernard, Gillibert, R., Yasukuni, Ryohei, Lamy De La Chapelle, Marc, Olivéro, Aurore, Sarkar, Mitradeep, Besbes, Mondher, Maillart, Emmanuel, Moreau, Julien, Canva, Michael, INL - Chimie et Nanobiotechnologies (INL - C&N), Institut des Nanotechnologies de Lyon (INL), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Lyon (ECL), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Institut d'électronique fondamentale (IEF), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques (CSPBAT), Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Charles Fabry / Biophotonique, Laboratoire Charles Fabry (LCF), Université Paris-Sud - Paris 11 (UP11)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS), and HORIBA Scientific [France]

Subjects
[CHIM.MATE]Chemical Sciences/Material chemistry, [CHIM.OTHE]Chemical Sciences/Other, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

14. Influence of probe sequence and strand lenght on the morphology and structuration of DNA chip

Author

Rouillat, M. H., Vincent Dugas, Federica Calevro, Cloarec, J. P., Hubert CHARLES, Magali Phaner-Goutorbe, Sciences Analytiques (SA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Biologie Fonctionnelle, Insectes et Interactions (BF2I), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA), INL - Chimie et Nanobiotechnologies (INL - C&N), Institut des Nanotechnologies de Lyon (INL), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-École Centrale de Lyon (ECL), Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), and Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)

Subjects
[PHYS]Physics [physics], probe, microarray, [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2004

15. Immobilisation of oligo-peptidic probes for microarray implementation : Characterisation by FTIR, Atomic Force Microscopy and 2D fluorescence

Author

Soultani-Vigneron, S., Dugas, V., Rouillat, M. H., Fedolliere, J., Duclos, M. C., Vnuk, E., Phaner-Goutorbe, M., Bulone, Vincent, Martin, J. R., Wallach, J., Cloarec, J. P., Soultani-Vigneron, S., Dugas, V., Rouillat, M. H., Fedolliere, J., Duclos, M. C., Vnuk, E., Phaner-Goutorbe, M., Bulone, Vincent, Martin, J. R., Wallach, J., and Cloarec, J. P.

Abstract

Proteomic microarrays show a wide range of applications for the investigation of DNA-protein, enzyme-substrate as well as protein-protein interactions. Among many challenges to build a viable protein microarray, the surface chemistry that will allow to immobilised various proteins to retain their biological activity is of paramount importance. Here we report a chemical functionalisation method allowing immobilisation of oligo-peptides onto silica surface (porous silica, glass, thermal silicon dioxide). Substrates were first derivatised with a monofunctional silane allowing the elaboration of dense and uniform monolayers in highly reproducible way. Prior to the oligo-peptides grafting, this organic layer was functionalised with an amino-polyethyleneglycol. The coupling step of oligo-peptides onto functionalised supports is achieved through activation of the C-terminal function of the oligo-peptides. Chemical surface modifications were followed by FTIR spectroscopy, AFM measurements and fluorescence scanning microscopy. A systematic study of the oligo-peptide grafting conditions (time, concentration, solvent) was carried out to optimise this step. The oligo-peptides grafting strategy implemented in this work ensure a covalent and oriented grafting of the oligo-peptides. This orientation is ensured through the use of fully protected peptide except the terminal primary an-tine. The immobilized peptides will be then deprotected before biological recognition. This strategy is crucial to retain the biological activity of thousands of oligo-probes assessed on a microarray., QC 20100525

Published
2005
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20. Oligosaccharides-Protein Interaction Study using Microarrays with DDI Immobilisation.

Author

Goudot, A., Zhang, J., Pourceau, G., Meyer, A., Cloarec, J-P., Rouanet, S., Vidal, S., Vasseur, J-J., Morvan, F., Chevolot, Y., and Souteyrand, E.

Abstract

Abstract: Carbohydrate and glycoconjugates play a major role in key biological events such as cell-cell recognition, pathogenesis and inflammation. As a consequence, there is a need to understand the structural parameters governing the recognition of carbohydrate by their receptors. Technologies for rapid monitoring and evaluating such interactions are of great importance to provide deep insights relevant to carbohydrate involving biological events. However, most conventional approaches are cumbersome and material and time consuming. Carbohydrate microarray technology is a promising approach for probing carbohydrate/protein interactions because it permits the simultaneous screening of a number of biological interactions with only minute amounts of material. Herein, we develop a strategy to probe different glycoconjugates (different spatial configuration, linker, geometry…) toward proteins in one time on a DNA Directed Immobilization (DDI) oligosaccharide biochip. [Copyright &y& Elsevier]

Published
2012
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21. Photopotential Imaging on Functionalized Surfaces Dedicated to Label-Free Detection of Biomolecular Interactions.

Author

Charrier, D., Chevolot, Y., Cloarec, J.-P., Goudot, A., Géhin, T., Roux, C., Dugas, V., Vidal, S., Vasseur, J.-J., Morvan, F., and Souteyrand, E.

Abstract

Abstract: We present a label-free biosensor capable to map local potential by photogenerating charge carriers in a field effect device. It consists in an amplitude-modulated laser beam scanning through an electrolyte/insulator/semiconductor structure at very low illumination around the equilibrium state of semiconductor. A proof of principle is demonstrated by depositing a self-assembled monolayer on the dielectric and by showing a clear photopotential change between naked and covered areas. [Copyright &y& Elsevier]

Published
2012
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23. PtCl<INF>2</INF>-Catalyzed Transannular Cycloisomerization of 1,5-Enynes:  A New Efficient Regio- and Stereocontrolled Access to Tricyclic Derivatives

Author

Blaszykowski, C., Harrak, Y., Goncalves, M.-H., Cloarec, J.-M., Dhimane, A.-L., Fensterbank, L., and Malacria, M.

Abstract

Transannular PtCl2-catalyzed cycloisomerizations open a new route to cyclopropanic tricyclic systems. Ketones A or C were efficiently prepared from the same cycloundec-5-en-1-yne precursor B, depending on the substituent at the propargylic position (either benzoate or methoxy).

Published
2004

27. Assessment of porous silicon substrate for well-characterised sensitive DNA chip implement.

Author

Bessueille F, Dugas V, Vikulov V, Cloarec JP, Souteyrand E, and Martin JR

Subjects
DNA genetics, Equipment Design, Equipment Failure Analysis, Materials Testing, Oligonucleotide Array Sequence Analysis methods, Porosity, Reproducibility of Results, Sensitivity and Specificity, Silicon analysis, Surface Properties, Crystallization methods, DNA analysis, DNA chemistry, Oligonucleotide Array Sequence Analysis instrumentation, Silicon chemistry
Abstract

A biochip approach based on porous silicon as substrate is presented. The goal is to enhance the sensitivity of the biochip by increasing the specific surface area on the support. The elaboration of porous silicon layers has been optimized to guarantee good accessibility for large bio-molecule targets. Oligonucleotide probes are synthesised directly on the surface using phosphoramidite chemistry. The high specific surface area of porous silicon allows the direct characterisation, by infrared spectroscopy, of the porous layer formation and the functionalisation steps. The monolayer grafting and derivatisation protocol is additionally characterized by wettability and fluorescence microscopy. The surface modification of porous layers (i.e. thermal oxidation and chemical derivatisation) ensures the stability of the structure against strong chemical reagents used during the direct oligonucleotide synthesis. Finally the protocol is successfully transferred to a flat Si/SiO(2) substrate, and validated by biological target specific recognition during hybridisation tests. In particular, radioactive measurements show a 10-fold enhancement of the oligonucleotide surface density on the porous silicon substrate compared to the flat thermal silica.

Published
2005
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28. Immobilisation of oligo-peptidic probes for microarray implementation: characterisation by FTIR, atomic force microscopy and 2D fluorescence.

Author

Soultani-Vigneron S, Dugas V, Rouillat MH, Fédollière J, Duclos MC, Vnuk E, Phaner-Goutorbe M, Bulone V, Martin JR, Wallach J, and Cloarec JP

Subjects
Biotinylation, Fluorescence, Glass chemistry, Microscopy, Atomic Force, Silicon Dioxide chemistry, Solvents chemistry, Spectroscopy, Fourier Transform Infrared, Surface Properties, Oligopeptides chemistry, Protein Array Analysis methods
Abstract

Proteomic microarrays show a wide range of applications for the investigation of DNA-protein, enzyme-substrate as well as protein-protein interactions. Among many challenges to build a viable "protein microarray", the surface chemistry that will allow to immobilised various proteins to retain their biological activity is of paramount importance. Here we report a chemical functionalisation method allowing immobilisation of oligo-peptides onto silica surface (porous silica, glass, thermal silicon dioxide). Substrates were first derivatised with a monofunctional silane allowing the elaboration of dense and uniform monolayers in highly reproducible way. Prior to the oligo-peptides grafting, this organic layer was functionalised with an amino-polyethyleneglycol. The coupling step of oligo-peptides onto functionalised supports is achieved through activation of the C-terminal function of the oligo-peptides. Chemical surface modifications were followed by FTIR spectroscopy, AFM measurements and fluorescence scanning microscopy. A systematic study of the oligo-peptide grafting conditions (time, concentration, solvent) was carried out to optimise this step. The oligo-peptides grafting strategy implemented in this work ensure a covalent and oriented grafting of the oligo-peptides. This orientation is ensured through the use of fully protected peptide except the terminal primary amine. The immobilized peptides will be then deprotected before biological recognition. This strategy is crucial to retain the biological activity of thousands of oligo-probes assessed on a microarray.

Published
2005
Full Text
View/download PDF

29. Optimisation of a silicon/silicon dioxide substrate for a fluorescence DNA microarray.

Author

Bras M, Dugas V, Bessueille F, Cloarec JP, Martin JR, Cabrera M, Chauvet JP, Souteyrand E, and Garrigues M

Subjects
Computer Simulation, Equipment Design, Equipment Failure Analysis, Oligonucleotide Array Sequence Analysis methods, Reproducibility of Results, Sensitivity and Specificity, Spectrometry, Fluorescence methods, Coated Materials, Biocompatible chemistry, Computer-Aided Design, Models, Chemical, Oligonucleotide Array Sequence Analysis instrumentation, Silicon chemistry, Silicon Dioxide chemistry, Spectrometry, Fluorescence instrumentation
Abstract

This paper presents a comprehensive theory and experimental characterisation of the modulation of the fluorescence intensity by the construction of optical interferences on oxidised silicon substrates used for DNA microarrays. The model predicts a 90-fold variation of the fluorescence signal depending on the oxide thickness. For a Cy3 dye, the signal is maximal for a 90 nm oxide thickness corresponding to a 7.5-fold enhancement with respect to a standard glass substrate. For experimental validation of the model, we have prepared Si/SiO2 substrates with different parallel steps of decreasing oxide thicknesses on the same sample using a buffered oxide etch (BOE) etching process after thermal oxidation. The SiO2 surface has been functionalized by a silane monolayer before in situ synthesis of L185 oligonucleotide probes. After hybridisation with complementary targets, the variations of the fluorescence intensity versus oxide thickness are in very good accordance with the theoretical model. The experimental comparison against a glass substrate shows a 10-fold enhancement of the detection sensitivity. Our results demonstrate that a Si/SiO2 substrate is an attractive alternative to standard glass slides for the realisation of fluorescence DNA microarrays whenever detection sensitivity is an important issue.

Published
2004
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30. Implementation of DNA chips obtained by microprojection for diagnostic and personalized medicine.

Author

Cabrera M, Jaber M, Dugas V, Broutin J, Vnuk E, Cloarec JP, Souteyrand E, and Martin JR

Subjects
Equipment Design, Genetic Diseases, Inborn diagnosis, Genetic Testing economics, Humans, Molecular Diagnostic Techniques methods, Molecular Structure, Oligonucleotide Array Sequence Analysis economics, Oligonucleotide Array Sequence Analysis methods, Silanes chemistry, Molecular Diagnostic Techniques instrumentation, Oligonucleotide Array Sequence Analysis instrumentation, Oligonucleotide Probes chemical synthesis
Abstract

It is expected that rapidly emergent new fields of application for DNA chips will be Diagnostic and Personalized Medicine. These new applications will require a limited number of probes, generally from 100 to 1000. So, after a brief review of the existing techniques to manufacture DNA chips, which are efficient for R&D applications and which often require a higher number of probes, we shall first report some advances in the silanization of the substrates and the grafting of probes to improve the robustness and the reliability of the devices. Then we shall discuss two manufacturing processes working at the scale of a nanoliter of reactant: ex situ and in situ fabrication by microprojection. We shall see how these processes are complementary and may be used to design and produce chips, at a large scale, for these new applications.

Published
2004

31. Immobilization of homooligonucleotide probe layers onto Si/SiO(2) substrates: characterization by electrochemical impedance measurements and radiolabelling.

Author

Cloarec JP, Deligianis N, Martin JR, Lawrence I, Souteyrand E, Polychronakos C, and Lawrence MF

Subjects
Electric Impedance, Electrochemistry, Radioisotopes, Silanes, Silicon, Silicon Dioxide, Biosensing Techniques, Molecular Probe Techniques, Oligonucleotide Probes
Abstract

Radiolabelling and electrochemical impedance measurements were used to characterize the immobilization of single stranded homooligonucleotides onto silica surfaces and their subsequent hybridization with complementary strands. The immobilization procedure consists of grafting an epoxysilane onto microelectronic grade Si/SiO(2) substrates, and coupling oligonucleotides bearing a hexylamine linker onto the epoxy moiety. Radiolabelling was used as a reference method to quantify the amount of immobilized and hybridized oligonucleotides. These results show that the Si/SiO(2) substrates modified with an epoxysilane yield a surface concentration of approximately 10(11) strands/cm(2) for the immobilized oligonucleotides, after vigorous washings, and that approximately 36% of these undergo hybridization with complementary strands. The impedance measurements, which provide a direct means of detecting variations in electrical charge accumulation across the semiconductor/oxide/electrolyte structure when the oxide surface is chemically modified, show that the semiconductor's flat band potential undergoes reproducible shifts of -150 and -100 mV following the immobilization and the hybridization step, respectively. These results demonstrate that electrochemical impedance measurements using chemically modified semiconductor/oxide/electrolyte structures of this type offer a viable alternative for the direct detection of complementary DNA strands upon hybridization.

Published
2002
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