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1. Staphylococcus aureus Phenol-Soluble Modulins Impair Interleukin Expression in Bovine Mammary Epithelial Cells

Author

Ksenia Semenovskaya, Pierre Germon, Martine Deplanche, Yves Le Loir, Chih-Lung Fu, Frederic Dessauge, Liudmila Alekseeva, Pascal Rainard, David Ge Smith, Laurence Finot, Nadejda Berkova, Holm Zerbe, Michael Otto, Wolfram Petzl, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Russian Academy of Sciences, National Institutes of Health [Bethesda] (NIH), Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), Clinic for Ruminants at the Centre for Clinical Veterinary Medicine, Ludwig Maximilians University of Munich, UR Infectiologie animale et Santé publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Infectiologie Santé Publique (ISP-311), Université de Tours-Institut National de la Recherche Agronomique (INRA), Institute of Infection, Immunity and Inflammation, University of Glasgow, ERANETANIHWA (KOLIMASTIR Convention ANR-13-ANWA-0003-06)., Laboratory of Bacteriology, US National Institutes of Health, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), the Russian Academy of Sciences [Moscow, Russia] (RAS), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université de Tours, and ProdInra, Archive Ouverte

Subjects
interleukine, 0301 basic medicine, Il-6, Il-8, medicine.disease_cause, Regulation of gene expression, Virulence, interleukin, santé animale, cellule épithéliale, Interleukin, immunologic reactions, 3. Good health, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Infectious Diseases, Staphylococcus aureus, Host-Pathogen Interactions, phenol-soluble modulins, Female, escherichia coli, medicine.symptom, Signal Transduction, staphylococcus aureus, Bacterial Toxins, réponse immunitaire, 030106 microbiology, Immunology, Inflammation, Biology, mastitis, Microbiology, Cell Line, 03 medical and health sciences, Mammary Glands, Animal, Immune system, Species Specificity, medicine, Animals, Interleukin 8, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Cellular Microbiology: Pathogen-Host Cell Molecular Interactions, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, animal health, Interleukin-6, Il-32, Interleukins, epithelial cell, Genetic Complementation Test, Interleukin-8, infection bactérienne, [SDV.BA.MVSA] Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Epithelial Cells, Dendritic cell, mammite, 030104 developmental biology, Gene Expression Regulation, Cell culture, Cattle, Parasitology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

The role of the recently described interleukin-32 (IL-32) in Staphylococcus aureus -induced mastitis, an inflammation of the mammary gland, is unclear. We determined expression of IL-32, IL-6, and IL-8 in S. aureus - and Escherichia coli -infected bovine mammary gland epithelial cells. Using live bacteria, we found that in S. aureus -infected cells, induction of IL-6 and IL-8 expression was less pronounced than in E. coli -infected cells. Notably, IL-32 expression was decreased in S. aureus -infected cells, while it was increased in E. coli -infected cells. We identified the staphylococcal phenol-soluble modulin (PSM) peptides as key contributors to these effects, as IL-32, IL-6, and IL-8 expression by epithelial cells exposed to psm mutant strains was significantly increased compared to that in cells exposed to the isogenic S. aureus wild-type strain, indicating that PSMs inhibit the production of these interleukins. The use of genetically complemented strains confirmed this observation. Inasmuch as the decreased expression of IL-32, which is involved in dendritic cell maturation, impairs immune responses, our results support a PSM-dependent mechanism that allows for the development of chronic S. aureus -related mastitis.

Published
2016
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2. [i]Staphylococcus aureus[/i] phenol-soluble modulins impair interleukin expression in bovine mammary epithelial cells

Author

Martine Deplanche, Liudmila Alekseeva, Ksenia Semenovskaya, Chih-Lung Fu, Frederic Dessauge, Laurence Finot, Wolfram Petzl, Holm Zerbe, Yves Le Loir, Pascal Rainard, David Ge Smith, Pierre GERMON, Michael Otto, Nadejda Berkova, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Shemyakin-Ovchinnikov Institute of Bio-organic Chemistry, Laboratory of Bacteriology, US National Institutes of Health, Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA), Clinic for Ruminants at the Centre for Clinical Veterinary Medicine, University Munich, Unversity of Munich, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institute of Infection, Immunity and Inflammation, University of Glasgow, Institut National de Recherche Agronomique (INRA). UMR Infectiologie et Santé Publique (1282)., AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), UR Infectiologie animale et Santé publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Infectiologie Santé Publique (ISP-311), Université de Tours-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
staphylococcus aureus, interleukine, cytokinine, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, réponse immunitaire, [SDV.IDA]Life Sciences [q-bio]/Food engineering, santé animal, escherichia coli, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, infection, mammite
Abstract

While the role of many cytokines during infection is revealed, the implication of several recently discovered cytokines during infection is not completely understood. The involvement of currently described interleukin-32 (IL-32) in infectious mastitis (an inflammation of the mammary gland), caused by two prevalent mastitis pathogens, Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) , was not investigated so far. We determined expression of IL-32, IL-6 and IL-8 in S. aureus- as compared to E.coli-infected bovine mammary gland epithelial cells. Using live bacteria we found that in S. aureus-infected cells, induction of IL-6 and IL-8 expression was less pronounced than in E. coli-infected cells. Notably, IL-32 expression was decreased in S. aureus-infected cells, while it was increased in E. coli-infected cells. IL-32 expression in E. coli- and S. aureus-infected udder tissue resembles in vitro responses. We identified the staphylococcal phenol-soluble modulin (PSM) peptides as key contributors to these effects, as IL-32, IL-6 and IL-8 expression by epithelial cells exposed to psm mutant strains was significantly increased as compared to cells exposed to the isogenic S. aureus wild-type strain, indicating that PSMs inhibit the production of these interleukins. The use of genetically complemented strains confirmed this observation. Inasmuch as the decreased expression of IL-32, which is involved in dendritic cell maturation, impairs immune responses, our results support a PSM-dependent mechanism that allows for the development of chronic S aureus-related mastitis.

Published
2016

3. Staphylococcus aureus Phenol-Soluble Modulins Impair Interleukin Expression in Bovine Mammary Epithelial Cells.

Author

Deplanche M, Alekseeva L, Semenovskaya K, Fu CL, Dessauge F, Finot L, Petzl W, Zerbe H, Le Loir Y, Rainard P, Smith DGE, Germon P, Otto M, and Berkova N

Subjects
Animals, Bacterial Toxins genetics, Bacterial Toxins toxicity, Cattle, Cell Line, Epithelial Cells drug effects, Epithelial Cells pathology, Escherichia coli genetics, Escherichia coli growth & development, Female, Gene Expression Regulation, Genetic Complementation Test, Interleukin-6 genetics, Interleukin-6 immunology, Interleukin-8 genetics, Interleukin-8 immunology, Interleukins immunology, Mammary Glands, Animal immunology, Mammary Glands, Animal pathology, Signal Transduction, Species Specificity, Staphylococcus aureus genetics, Staphylococcus aureus growth & development, Virulence, Bacterial Toxins biosynthesis, Epithelial Cells microbiology, Host-Pathogen Interactions, Interleukins genetics, Staphylococcus aureus pathogenicity
Abstract

The role of the recently described interleukin-32 (IL-32) in Staphylococcus aureus-induced mastitis, an inflammation of the mammary gland, is unclear. We determined expression of IL-32, IL-6, and IL-8 in S. aureus- and Escherichia coli-infected bovine mammary gland epithelial cells. Using live bacteria, we found that in S. aureus-infected cells, induction of IL-6 and IL-8 expression was less pronounced than in E. coli-infected cells. Notably, IL-32 expression was decreased in S. aureus-infected cells, while it was increased in E. coli-infected cells. We identified the staphylococcal phenol-soluble modulin (PSM) peptides as key contributors to these effects, as IL-32, IL-6, and IL-8 expression by epithelial cells exposed to psm mutant strains was significantly increased compared to that in cells exposed to the isogenic S. aureus wild-type strain, indicating that PSMs inhibit the production of these interleukins. The use of genetically complemented strains confirmed this observation. Inasmuch as the decreased expression of IL-32, which is involved in dendritic cell maturation, impairs immune responses, our results support a PSM-dependent mechanism that allows for the development of chronic S. aureus-related mastitis., (Copyright © 2016, American Society for Microbiology. All Rights Reserved.)

Published
2016
Full Text
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