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Objective To describe the pharmacokinetics and pharmacodynamics of meperidine after IM and subcutaneous administration in horses. Study design prospective, randomized, blinded, crossover trial. Animals Six adult horses weighing 494 ± 33 kg. Methods Treatments included meperidine 1 mg/kg IM with saline 6 mL subcutaneously, meperidine 1 mg/kg subcutaneously with saline 6 mL IM, and saline 6 mL subcutaneously and 6 mL IM, with a 7-day washout between treatments. Plasma meperidine concentrations and pharmacodynamic values (thermal and mechanical thresholds, physiological variables, fecal production) were collected at various time points for 24 hours. Accelerometry data were obtained for 8 hours to measure locomotor activity. Data were analyzed with a mixed effects model, and α was set at .05. Results Meperidine terminal half-life (T1/2 ), maximal plasma concentrations, and time to maximal concentration were 186 ± 59 and 164 ± 56 minutes, 265.7 ± 47.2 and 243.1 ± 80.1 ng/mL at 17 ± 6, and 24 ± 13 minutes for IM at subcutaneous administration, respectively. No effect of treatment or time was observed on thermal or mechanical thresholds, heart rate, respiratory rate, locomotor activity, frequency of defecations, or fecal weight (P > .2 for all). Conclusion Maximum meperidine concentrations were achieved quickly with a short T1/2 in both treatment groups. Neither IM nor subcutaneous meperidine influenced thermal or mechanical threshold or physiological variables. Clinical significance The short half-life and lack of detectable antinociceptive effect do not support IM or subcutaneous administration meperidine at 1 mg/kg for analgesia in horses.

Chronic lung disease (CLD) is common in individuals living with perinatally acquired HIV (PA-HIV) in southern/eastern Africa. Most of the UK PA-HIV population are African. We conducted a case-note review of CLD in 3 UK PA-HIV cohorts (n = 98). Bronchiectasis or obliterative bronchiolitis occurred in 8.1% of patients and ring/tramline opacities occurred in 19.2% of patients on chest radiograph. There may be unrecognized and underdiagnosed CLD among PA-HIV in the UK.

Four dams on the lower Snake River in Washington State generate hydropower and allow for regional agriculture and barge shipping to Portland OR. However, the dams impede the migration of local salmon populations (Oncorhynchus spp.), which are in steep decline, and drastically impact the populations of salmon and orca whales, for whom salmon are a primary food source. For years, environmental groups have argued for breaching the dams; other interests counter that the dams are too critical to the economy of the region to lose; and federal agencies assert that the dams can remain and salmon populations will recover with mitigation techniques. Scientific and economic analyses, litigation, and elected officials’ efforts have not been able to move the issue towards a solution. Readers will examine the interests of primary actors in the issue, how they influence the policy process, the role of scientific and economic analyses, and possible approaches for resolving the issue.

Objective Describe the pharmacokinetics and pharmacodynamics of intravenous hydromorphone in healthy horses. Study design Masked, randomized, cross-over, Latin square design. Animals A group of eight healthy adult horses Methods Horses were administered each of four treatments with an 8 day washout. Treatments groups included intravenous hydromorphone 0.02 mg kg–1 (LD), 0.04 mg kg–1 (MD), 0.08 mg kg–1 (HD) and saline (P). Blood samples for hydromorphone analysis were obtained for 24 hours after treatment. Plasma hydromorphone was quantified and pharmacokinetic parameters were determined using non-compartmental analysis. Pharmacodynamic data collected for 24 hours after treatment included thermal nociceptive threshold, heart rate (HR), respiratory rate (fR) and rectal temperature, and analyzed using mixed-effects linear models. Results Mean (± standard deviation) hydromorphone terminal half-life (t1/2), systemic clearance and apparent volume of distribution at steady state (Vdss) were 18.1 ± 18.6, 34.0 ± 12.8, and 41.3 ± 32.5 minutes, 66.6 ± 5.3, 550.0 ± 76.4, and 92.7 ± 13.9 mL kg–1 minute–1, and 1118 ± 369, 1460 ± 325 and 2242 ± 950 mL kg–1 for treatments LD, MD and HD, respectively. Thermal threshold increased significantly compared to baseline for all treatments for up to 12 hours. HR was elevated above baseline in treatments LD, MD and HD, extending to 30, 15 and 105 minutes after treatment, respectively. Respiratory rate was elevated above baseline in treatments MD and HD from 30 to 195 minutes and from 45 to 480 minutes after treatment, respectively. Temperature was elevated above baseline in treatment HD until 255 minutes after treatment. Conclusions Hydromorphone exhibited a short t1/2, rapid clearance and large Vdss in horses. It also provided a dose-dependent increase in thermal threshold with associated increases in HR, fR and rectal temperature. Clinical relevance Hydromorphone 0.04 mg kg–1 provided clinically relevant thermal antinociception with minimal adverse effects.

The sweat test is the gold standard for the diagnosis of cystic fibrosis (CF). The test utilizes iontophoresis to administer pilocarpine to the skin to induce sweating for measurement of chloride concentration in sweat. However, the sweat test procedure needs to be conducted in an accredited lab with dedicated instrumentation, and it can lead to inadequate sweat samples being collected in newborn babies and young children due to variable sweat production with pilocarpine iontophoresis. We tested the feasibility of using microneedle (MN) patches as an alternative to iontophoresis to administer pilocarpine to induce sweating. Pilocarpine‐loaded MN patches were developed. Both MN patches and iontophoresis were applied on horses to induce sweating. The sweat was collected to compare the sweat volume and chloride concentration. The patches contained an array of 100 MNs measuring 600 μm long that were made of water‐soluble materials encapsulating pilocarpine nitrate. When manually pressed to the skin, the MN patches delivered >0.5 mg/cm2 pilocarpine, which was double that administered by iontophoresis. When administered to horses, MN patches generated the same volume of sweat when normalized to drug dose and more sweat when normalized to skin area compared to iontophoresis using a commercial device. Moreover, both MN patches and iontophoresis generated sweat with comparable chloride concentration. These results suggest that administration of pilocarpine by MN patches may provide a simpler and more‐accessible alternative to iontophoresis for performing a sweat test for the diagnosis of CF.

Cephalosporin antimicrobials can be utilized for the treatment of sepsis in neonatal foals, particularly when an aminoglycoside is contraindicated. Some cephalosporins, however, are not utilized because of cost, sporadic availability, or uncertainty about efficacy. The plasma disposition of ceftazidime, a third-generation cephalosporin with a broad spectrum of activity against a wide variety of gram-negative bacteria and minimal renal side effects has not been reported in neonatal foals. In this study, the plasma disposition of single intravenous (IV) and intramuscular (IM) doses of ceftazidime in neonatal foals was determined. Six healthy one to two-day-old foals were given 25 mg/kg of ceftazidime by IV and IM routes in a cross-over design, with a 48-h washout period between doses. Non-compartmental analysis was used to estimate plasma pharmacokinetic parameters. Median t1/2 was 2 h and median AUC0-last was 364 µg h/ml for both IV and IM administration. Median Cmax after IM administration was 101 µg/ml, with a median Tmax of 0.7 h. Relative bioavailability of IM injection was 90%. There were no statistically significant differences between estimated IV and IM pharmacokinetic parameters. Plasma concentrations remained above the human CLSI susceptible breakpoint for Enterobacteriaceae for over 8 h following IV and IM administration.

Local-level hazard mitigation plans, particularly in coastal areas, are critical to protecting communities from natural hazards, some of which are expected to increase with climate change. ...

OBJECTIVE To determine the effect of xylazine on intracranial pressure (ICP) in standing compared to isoflurane-anesthetized horses. DESIGN Prospective, crossover study design. SETTING University Teaching Hospital. ANIMALS Six adult horses donated to the University. Horses were determined to be healthy via physical examination, complete blood count, and neurological evaluation. INTERVENTIONS Horses were anesthetized, maintained on isoflurane in oxygen in left lateral recumbency, and ventilated to normocapnia. Horses were instrumented for intraparenchymal measurement of ICP, invasive blood pressure, pulse oximetry, and end tidal gas analyzer. Xylazine 1 mg/kg was administered IV and ICP, systolic arterial pressure, mean arterial pressure (MAP), diastolic arterial pressure, and heart rate were recorded and cerebral perfusion pressure (CPP) was calculated for the following 15 minutes. Twenty-four to 36 hours following anesthetic recovery, xylazine 1 mg/kg was administered IV and ICP, heart rate, and Doppler blood pressure (BPdop) on the tail were monitored for 15 minutes. MEASUREMENTS AND MAIN RESULTS There was a decrease in ICP following administration of xylazine in anesthetized horses (P

Following the grounding of the Exxon Valdez in 1989, a sustainable human use framework (human use framework) for Prince William Sound (PWS), AK was developed by the Chugach National Forest after concerns emerged about the social and environmental impacts of expanding human use due to cleanup activities and increased recreation visitation. A practical, issue-based process was used to develop an implementation strategy for integrating the complex social and ecological data contained in the human use framework and for making specific management and monitoring recommendations. The issue-based process is more of a bottom-up approach to planning compared to typical top-down planning approaches that seek to impose or replicate system-wide environmental conditions as criteria for selecting management and monitoring practices. The implementation strategy includes management guidance for protecting "keystone" recreation experiences while simultaneously protecting social and ecological values of PWS. It also includes practical social and ecological monitoring recommendations that can be used to inform an adaptive management strategy for continued management of sustainable human uses. The issue-based process serves as a model for other coastal zone areas struggling to balance increasing human use and recreation with conservation and environmental protection goals.

As approaches to ecological restoration become increasingly large scale and collaborative, there is a need to better understand social aspects of restoration and how they influence land management. In this article, we examine social perspectives that influence the determination of ecological reference conditions in restoration. Our analysis is based on in‐depth interviews with diverse stakeholders involved in collaborative restoration of fire‐adapted forest landscapes. We conducted interviews with 86 respondents from six forest collaboratives that are part of the U.S. Forest Service's Collaborative Forest Landscape Restoration Program. Collaboratives use a variety of approaches to develop reference conditions, including historic, contemporary, and future scenarios. Historical conditions prior to European settlement (nineteenth century or “pre‐settlement” conditions), or prior to more recent grazing, logging, and exclusion of fire, were the predominant type of reference used in all sites. Stakeholders described benefits and limitations of reference conditions. Primary benefits include (1) providing a science‐based framework for bringing stakeholders together around a common vision; (2) gaining social understanding and acceptance of the underlying need for restoration; and (3) serving to neutralize otherwise value‐laden discussions about multiple, sometimes competing, resource objectives. Limitations stem from (1) concerns over social conflict when reference conditions are perceived to contradict other stakeholder values and interests, (2) differing interpretations of reference condition science, (3) inappropriate application or over‐generalization of reference information, and (4) limited relevance of historical references for current and future conditions in some ecosystems. At the same time, collaboratives are adopting innovative strategies to address conceptual and methodological limitations of reference conditions.

The ecological outcomes of civic environmental stewardship are poorly understood, especially at scales larger than individual sites. In this study we characterized civic environmental stewardship programs in the Green-Duwamish watershed in King County, WA, and evaluated the extent to which stewardship outcomes were monitored. We developed a four-step process based on the Open Standards for the Practice of Conservation to structure our evaluation and to make recommendations for future monitoring of ecological outcomes of stewardship activities. Environmental stewardship, primarily in the form of restoration projects, was common throughout the lower and middle watershed. The distribution of stewardship sites was influenced by population density, political and program boundaries, and financial and technical resources. Conceptual frameworks that link conservation goals, ecological threats, management strategies, and monitoring were rare and incomplete. Collaboration among programs was an important component of stewardship in the watershed, although communication gaps were identified between geographic regions and different ecosystems. Monitoring efforts were relatively common but unevenly distributed, often unsystematic, and usually dictated by project maintenance, funding purpose, or a program’s standard practices, rather than specific ecological outcomes and goals. As a result, monitoring results were rarely and inconsistently used for management. We recommend improving stewardship monitoring by clearly linking stewardship activities to specific conservation goals and objectives, developing reasonable quantitative outcome metrics that link upland and aquatic environments, and improving coordination and learning of monitoring efforts among multiple stewardship programs and actors.

Digging animals may alter many characteristics of their environment as they disrupt and modify the ground's surface by creating foraging pits or burrows. Extensive disturbance to the soil and litter layer changes litter distribution and availability, potentially altering fuel loads. In many landscapes, including peri-urban areas, fire management to reduce fuel loads is complex and challenging. The reintroduction of previously common digging animals, many of which are now threatened, may have the added benefit of reducing fuel loads. We experimentally examined how the reintroduction of a marsupial bandicoot, quenda (Isoodon fusciventer), altered surface fuel loads in an urban bush reserve in Perth, Western Australia. Foraging activities of quenda (where they dig for subterranean food) were substantial throughout the reserve, creating a visibly patchy distribution in surface litter. Further, in open plots where quenda had access, compared to fenced plots where quenda were excluded, quenda foraging significantly reduced litter cover and litter depth. Similarly, estimated surface fuel loads were nearly halved in open plots where quenda foraged compared to fenced plots where quenda were absent (3.6 cf. 6.4 Mg/ha). Fire behavior modeling, using the estimated surface fuel loads, indicated the predicted rate of spread of fire were significantly lower for open plots where quenda foraged compared to fenced plots under both low (29.2 cf. 51.4 m/h; total fuels) and high (74.3 cf. 130.4 m/h; total fuels) fire conditions. Although many environments require fire, including the bushland where this study occurred, fire management can be a considerable challenge in many landscapes, including urban bushland reserves, which are usually small and close to human infrastructure. The reintroduction of previously common digging species may have potential value as a complimentary tool for reducing fuel loads, and potentially, fire risk.

OBJECTIVE To determine whether an association exists between direct intracranial pressure (ICP) measurement and ultrasonographic measurement of optic nerve sheath diameter (ONSD) in anesthetized and standing horses. DESIGN Cross-sectional study performed on a convenience sample of healthy adult horses. SETTING University teaching hospital. ANIMALS Eight adult horses donated to the University. Enrolled horses were free of abnormalities on physical examination, CBC, neurological evaluation, and ophthalmological examination. MEASUREMENTS AND MAIN RESULTS Horses were anesthetized in lateral recumbency for placement of an ICP transducer. Three head positions (neutral, elevated, and lowered) were used to alter ICP. ICP and ONSD in 2 directions (D1 and D2) were recorded at 5 and 10 minutes after position change to elevated and lowered. ICP and ONSD measurements were repeated in standing sedated horses 24-36 hours after recovery from anesthesia. Linear regressions were performed with ICP as the dependent variable and ONSD as the independent variable by head position and times. Linear regressions were also performed for change from neutral under anesthesia, with ONSD as the independent variable and ICP as the dependent variable, by head position and times. Significance was set at P

Objective To compare the pharmacokinetics and pharmacodynamics of hydromorphone in horses after intravenous (IV) and intramuscular (IM) administration. Study design Randomized, masked, crossover design. Animals A total of six adult horses weighing [mean ± standard deviation (SD))] 447 ± 61 kg. Methods Horses were administered three treatments with a 7 day washout. Treatments were hydromorphone 0.04 mg kg⁻1 IV with saline administered IM (H-IV), hydromorphone 0.04 mg kg⁻1 IM with saline IV (H-IM), or saline IV and IM (P). Blood was collected for hydromorphone plasma concentration at multiple time points for 24 hours after treatments. Pharmacodynamic data were collected for 24 hours after treatments. Variables included thermal nociceptive threshold, heart rate (HR), respiratory frequency (fR), rectal temperature, and fecal weight. Data were analyzed using mixed-effects linear models. A p value of less than 0.05 was considered statistically significant. Results The mean ± SD hydromorphone terminal half-life (t1/2), clearance and volume of distribution of H-IV were 19 ± 8 minutes, 79 ± 12.9 mL minute⁻1 kg⁻1 and 1125 ± 309 mL kg⁻1. The t1/2 was 26.7 ± 9.25 minutes for H-IM. Area under the curve was 518 ± 87.5 and 1128 ± 810 minute ng mL⁻1 for H-IV and H-IM, respectively. The IM bioavailability was 217%. The overall thermal thresholds for both H-IV and H-IM were significantly greater than P (p Conclusions and clinical relevance IM hydromorphone has high bioavailability and provides a similar degree of antinociception to IV administration. IM hydromorphone in horses provides a similar degree and duration of antinociception to IV administration.

Shellfish aquaculture can result in conflicts among stakeholders who perceive impacts and tradeoffs regarding sense of place, aesthetic, recreational, economic, and ecological values. Pacific geoduck clams (Panopea generosa Gould 1850) are grown in intertidal plots using gear- and labor-intensive techniques that result in a high value export product. A confluence of issues has resulted in on-going social and legal tensions surrounding geoduck aquaculture in southern Puget Sound, Washington (WA), USA. Using interviews and document analysis, we explored stakeholder perspectives and policy issues related to geoduck aquaculture in southern Puget Sound. Twenty-three stakeholders were interviewed, including state agency employees, representatives of the aquaculture industry, nongovernmental organizations, landowners, a tribal member, and an academic. Nine state hearings board decisions on challenges to aquaculture permits were also analyzed. Stakeholders articulated a variety of perspectives regarding a...

Collaborative approaches to natural resource management are becoming increasingly common on public lands. Negotiating a shared vision for desired conditions is a fundamental task of collaboration and serves as a foundation for developing management objectives and monitoring strategies. We explore the complex socio-ecological processes involved in developing a shared vision for collaborative restoration of fire-adapted forest landscapes. To understand participant perspectives and experiences, we analyzed interviews with 86 respondents from six collaboratives in the western U.S., part of the Collaborative Forest Landscape Restoration Program established to encourage collaborative, science-based restoration on U.S. Forest Service lands. Although forest landscapes and group characteristics vary considerably, collaboratives faced common challenges to developing a shared vision for desired conditions. Three broad categories of challenges emerged: meeting multiple objectives, collaborative capacity and trust, and integrating ecological science and social values in decision-making. Collaborative groups also used common strategies to address these challenges, including some that addressed multiple challenges. These included use of issue-based recommendations, field visits, and landscape-level analysis; obtaining support from local agency leadership, engaging facilitators, and working in smaller groups (sub-groups); and science engagement. Increased understanding of the challenges to, and strategies for, developing a shared vision of desired conditions is critical if other collaboratives are to learn from these efforts.

Rhodococcus equi , a soil saprophyte, is a common cause of pneumonia in foals and a frequent opportunistic pathogen in immunosuppressed people. Because it is widespread in the environment, R. equi can be detected in the feces of most horses. However, the exact timing and rate of shedding relative to infection is unknown. The objectives of this study were to quantify shedding of R. equi in mares and foals after experimental infection of foals with 2 different inocula and to determine the effect of composting on concentrations of R. equi in contaminated bedding. Foals were infected intratracheally with virulent R. equi using inocula of 1 × 10 7 CFU/mL (n = 16) or 1 × 10 6 CFU/mL (n = 12) at 23 ± 2 days (range 21 to 27 days) of age. Fecal samples were collected from mares and foals prior to infection and on days 3, 7, and 14 post-infection for quantitative culture of total and virulent R. equi . Waste from the horses was composted for 7 days. Concentrations of total and virulent R. equi in foal feces were significantly higher on day 14 post-infection compared to day 0, regardless of inoculum size. Concentration of total R. equi in mare feces was significantly higher on days 3, 7 and 14 compared to day 0 regardless of inoculum size, whereas shedding of virulent R. equi only increased on day 14 post-infection. Composting for 7 days significantly decreased concentrations of total R. equi and virulent R. equi by an average of 1.08 ± 0.21 and 0.59 ± 0.26 log 10 CFU/g, respectively.

Urban forest management plans serve as a municipality's guiding document for management of its urban trees and urban forest. This paper presents results of a content analysis and evaluation of the comprehensiveness of 39 urban forest management plans in Washington State. Comprehensiveness is the degree to which a plan includes a review of the current state of the resource, a vision for the future state, goals and objectives, an action plan for implementation, and a plan for monitoring progress. We also explored whether municipality size, community involvement, plan author, or funding source influence plan comprehensiveness. Plan comprehensiveness varies, and although most plans included the results of a tree inventory, very few conducted a full assessment of the current state of the urban forest. Tree maintenance, tree establishment, and tree protection were addressed most frequently, and many of the plans included a vision statement. The majority of plans included detailed action steps for implementing goals related to tree maintenance and tree establishment, and about a quarter of the plans included an implementation plan. Very few plans addressed monitoring and adaptive management, and no plan included a detailed strategy for monitoring the implementation of the plan. Larger municipalities tend to have more comprehensive plans, and community involvement in the plan development process appears to positively influence the overall comprehensiveness score. No relationship was found between plan author or receipt of grant funding and plan comprehensiveness. Our approach for evaluating plan comprehensiveness suggests a useful framework for future plan development, revision, and evaluation.

Restoration of nearshore ecosystems presents many challenges for stakeholder involvement. Using surveys and interviews we examined stakeholder values, preferences, and potential coalitions surrounding nearshore restoration in the Whidbey sub-basin of Puget Sound. Most stakeholders in our study believe that Puget Sound nearshore problems are severe and urgent, and that it is worth investing in restoration. They do not agree on the causes of nearshore degradation, yet support stronger regulatory enforcement and increased public ownership as possible solutions to nearshore problems. Five potential stakeholder coalitions were identified based on shared values. These values reflect a varied spectrum of support for public sector solutions to nearshore problems and were labeled: No Government Intervention, Property Rights, Private Land Stewardship, Protect Undeveloped Areas, and Large Scale Restoration. The potential coalitions identified confirm the Advocacy Coalition Framework hypothesis that coalition members...

A Web-based survey of wildland fire managers in federal agencies in the western United States explored fire science information needs and sources, why particular sources are used, and barriers to obtaining and using information. The fire managers we surveyed rely heavily on internal agency information sources (colleagues, technical experts, and resource advisors) and are more likely to face barriers in their ability to access and use relevant information (lack of time, funding, and personnel) than problems with the quality or availability of data. Information accessibility and applicability are important to managers when deciding which information sources to use. Managers frequently access research provided by US Forest Service Research and Development specialists and university scientists through various publications, the Internet, and direct communication with scientists. Understanding the types of information fire managers need, the sources they access for information, and the barriers they face in obtaining and using information may lead to improved fire science and its dissemination, as well as more effective and efficient fire management.

Peripheral blood mononuclear cells (PBMCs) were obtained from 6 foals1 week of age, 6 foals between 3 and 4 months of age, and 10 adult horses. PBMCs were stimulated with concanavalin A (ConA) or calcium ionomycin-phorbol myristate acetate (CaI-PMA) and the frequency of interferon IFN-gamma and IL-4 secreting cells was measured using an equine-specific ELISPOT assay. The number of IFN-gamma secreting cells was significantly lower in both groups of foals than in adult horses regardless of the mitogen used for stimulation. The number of IFN-gamma secreting cells was significantly higher in cells stimulated with CaI-PMA than in cells stimulated with ConA. In cells stimulated with CaI-PMA, the number of IL-4 secreting cells was significantly lower in both groups of foals compared to adult horses. In adult horses only, CaI-PMA stimulation resulted in significantly more IL-4 secreting cells than ConA stimulation. Regardless of age, the ratio of IFN-gamma/IL-4 spot forming cells (SFC) was significantly higher in cells stimulated with CaI-PMA than in cells stimulated with ConA. These findings indicate that the frequency of IFN-gamma and IL-4 secreting cells is lower in foals than in adult horses and that the type of mitogen used has a profound effect on the relative production of both cytokines.

Promotion of effective science exchange between government scientists and managers requires thoughtful arrangement and operation of research and management functions. The U.S. Forest Service was established at the peak of the Progressive Era, when science exchange was designed to occur between researchers and resource managers who worked in distinct arms of the agency, but shared similar goals of effective forest management. In this article, the authors explore the implications of diminished agency capacity for science exchange interactions between researchers and managers in recreation management. Managers and researchers identified their current interactions, their perceptions of ideal interactions, and barriers to achieving those ideals. Reductions in agency capacity for recreation management have resulted in the erosion of interactions between managers and researchers. However, effective science exchange does occur, but requires innovative and adaptive approaches.

The HIV epidemic in Fiji remains largely uncharacterized. By February 2009, there were 294 confirmed cases; the majority occurred among the 20- to 39-year old age group and resulted from heterosexual contact. There are currently no published data concerning HIV subtypes in Fiji. In this study, venous blood samples were collected as dried blood spots from 35 HIV-positive individuals in Fiji. HIV-1 subtype was determined for 27 (77%) samples and the presence of four different subtypes, with multiple introductions of two, was demonstrated. Subtype distribution was as follows: 16 (59%) were subtype C, 9 (33%) were subtype B, 1 (4%) was subtype A, and 1 (4%) was subtype G. Phylogenetic analysis showed a clear segregation of the Fijian subtype C isolates and previously published Papua New Guinea subtype C isolates as well as multiple introductions of subtype B. These findings represent the first HIV-1 subtype data from the Fiji Islands.

The worldwide growth of urban settlements affects the management of natural resources and has prompted scholars in the natural and social sciences to call for ecosystem-based approaches to the management of human settlements. While considerable literature exists on the definition, theoretical underpinnings and methods for applying ecosystem-based management (EBM), few studies have examined whether urban and regional planners consider elements of EBM when developing environmental policy. This study assesses the extent to which planners apply EBM principles when reviewing scientific information for environmental policies in western Washington State. Using a working definition of EBM based on existing literature, the study conducts a content analysis of interview data from 42 environmental planners working for cities in western Washington, and asks what elements of EBM are considered as they review scientific information. The results suggest that elements related to monitoring, inter-agency co-operation, eco...

Over the last decade, new governance-style, collaborative approaches to environmental management have increased exponentially. What is not well known is how the structure of these partnerships, particularly the policies that foster their development, may contribute to theʼ ability to achieve either procedural or substantive policy goals. Our study investigated efforts in the States of Washington and Oregon to encourage the development of collaborative watershed management institutions. Thirtyfive watershed partnerships were examined to understand how the planning groups implement policy objectives and how partnerships are creating or modifying institutions for planning and implementation. Our findings suggest that both States face similar challenges in important respects. Challenges include obtaining adequate participation, developing and sustaining organizational capacity, and planning implementation. Together, these findings demonstrate that new governance strategies are not a panacea for water management.

Collaborative watershed partnerships are a common strategy for addressing complex natural resource management decisions. Despite a large literature surrounding their procedural strengths and weaknesses, little theory regarding collaborative partnership design is available to guide policymaking and implementation efforts. This study investigated the relationship between partnership structure and activities using interview data from 29 of Oregon's watershed partnerships. Confirming previous research, partnership composition is related to outcomes. Further exploration reveals that organizational affiliation is related to both composition and activities. Independent partnerships were more likely to conduct scientific assessments or plans, while agency-affiliated partnerships focused primarily on restoration projects. Additional findings suggest that independent partnerships develop priorities internally, while agency-affiliated partnerships tend to adopt the strategies of their parent organization. Diverse pa...

In 1998 the Washington State Legislature enacted the Watershed Planning Act, which encourages local governments to develop watershed plans using collaborative processes. Objectives of the statute are to address water resource and water quality issues, salmon habitat needs and to establish instream flows. This exploratory study sought to examine two aspects of how local governments are implementing the Act: challenges and benefits associated with collaborative watershed planning and the capacity of local governments to conduct collaborative watershed planning. Using documents and interview data from four cases, it was found that all planning groups experience similar challenges, although newer planning groups experienced more challenges than groups with previous planning experience. Challenges include issues surrounding the collaborative process, interagency co-ordination and trust. Local governments struggle with building capacity to plan, particularly in the areas of funding, technical expertise, incenti...

Collaborative approaches to natural resource management are becoming increasingly common on public lands. Negotiating a shared vision for desired conditions is a fundamental task of collaboration and serves as a foundation for developing management objectives and monitoring strategies. We explore the complex socio-ecological processes involved in developing a shared vision for collaborative restoration of fire-adapted forest landscapes. To understand participant perspectives and experiences, we analyzed interviews with 86 respondents from six collaboratives in the western U.S., part of the Collaborative Forest Landscape Restoration Program established to encourage collaborative, science-based restoration on U.S. Forest Service lands. Although forest landscapes and group characteristics vary considerably, collaboratives faced common challenges to developing a shared vision for desired conditions. Three broad categories of challenges emerged: meeting multiple objectives, collaborative capacity and trust, and integrating ecological science and social values in decision-making. Collaborative groups also used common strategies to address these challenges, including some that addressed multiple challenges. These included use of issue-based recommendations, field visits, and landscape-level analysis; obtaining support from local agency leadership, engaging facilitators, and working in smaller groups (sub-groups); and science engagement. Increased understanding of the challenges to, and strategies for, developing a shared vision of desired conditions is critical if other collaboratives are to learn from these efforts.

Regulatory negotiation is a process by which representatives of affected interests, along with the regulatory agency, attempt to negotiate a consensus agreement on the content of a regulation. This study represents an empirical effort to begin to identify and sort out the roles that an agency plays in a collaborative policy-making process such as regulatory negotiation. Three regulatory negotiation cases were examined to determine the perceived roles of the U.S. Environmental Protection Agency (EPA) officials and other participants, and the study suggests a number of findings. First, that EPA fulfills a number of different roles (Expert, Analyst, Stakeholder, Facilitator and Leader) in a negotiation process; second, that EPA is expected to be an active participant in the negotiations, not simply an overseer or facilitator of interests; and finally, EPA interprets its primary role much more narrowly – as that of an expert – than do other participants. Non-agency participants view EPA's primary role as a leader, which combines technical and substantive components as well as process components. Where previously and in the theoretical literature, agencies exercise leadership through statutory authority or technical expertise, this study suggests that there are additional dimensions to that leadership role. In a collaborative process such as regulatory negotiation, the agency finds itself in a realm that demands that they effectively merge the roles of expert, analyst, and stakeholder into a more complex leadership role than has been suggested in the past.

As the use of collaborative, consensus-based decisionmaking procedures increases, so, too, do the obstacles to using these processes. For example, many managers and decision makers are now encountering limits on the participation in such processes by key stakeholder groups. Indeed, perhaps one of the most difficult challenges an agency faces in undertaking a consensus process is in securing participation by appropriate stakeholders. In order to more effectively sponsor and manage collaborative decisionmaking processes, an understanding of the incentives for stakeholder participation is critical. A recent study of negotiated rulemaking at the US Environmental Protection Agency (USEPA) assessed participants' incentives for participation, which revolve around four major themes: (1) resources required for participation, (2) input into final decision and response to comments, (3) use of technical data, and (4) representation and communication issues. Keeping these incentives in mind when initiating a collaborative regulatory process may assist managers and sponsors in ensuring inclusion and participation by key participants.

As collaborative approaches to resolving public disputes become more prevalent, agency officials are finding themselves in unfamiliar terrain. This article offers one conceptualization of agency roles in collaborative processes, drawing from the experiences of natural resource managers and enviromental regulators at the federal level. Based on an examination of 65 cases, the authorsidentify three distinct "hat" that effective agency officals wear in collaborative processes - leader, partner, and stakeholder. The objective and function of each role is assessed, along with the implications of agency facilitation. The most successful agency representatives were able to blend the three primary roles, and did not act as facilitators of the collaborative process.

States, territories, and tribes identify nonpoint source pollution as responsible for more than half of the Nation’s existing and threatened water quality impairments, making it the principal remaining cause of water quality problems across the United States. Combinations of education, technical and financial assistance, and regulatory measures are used to inform landowners about nonpoint source pollution issues, and to stimulate the use of best management practices. A mail survey of non-commercial riparian landowners investigated how they learn about best management practices, the efficacy of different educational techniques, and what motivates them to implement land management activities. Landowners experience a variety of educational techniques, and rank those that include direct personal contact as more effective than brochures, advertisements, radio, internet, or television. The most important motivations for implementing best management practices were linked with elements of a personal stewardship ethic, accountability, personal commitment, and feasibility. Nonpoint source education and social marketing campaigns should include direct interpersonal contacts, and appeal to landowner motivations of caring, responsibility, and personal commitment.

The tasks of restoring rivers, watersheds, and other critical habitats are complex and represent some of the most difficult challenges faced by natural resource scientists and managers today. Blame for society’s inability to adequately deal with restoration challenges is placed on both scientists and policy makers. Some people argue that the necessary information and levels of certainty fall far short of scientific standards for decision making; others argue that science is not the issue, and indecisiveness merely reflects a lack of political leadership and will. Regardless, the discussion ultimately focuses on the science-policy interface as a root cause of the inability to address such complex management issues. Both science and policy hold unique cultural positions, values, and norms. When the two spheres try to communicate, these differences can interfere with developing, selecting, and implementing management alternatives. This chapter addresses the topic of science and policy communications, offers a conceptual framework to assist in understanding how scientists and policy makers might forge a new dialogue, and discusses additional institutional and individual constraints to restoration efforts. Examples of approaches toward overcoming these constraints provide hope for addressing and ultimately realizing the restoration challenges that lie ahead.

Cerveny, Lee K.; Ryan, Clare M. 2008. Agency capacity for recreation science and management: the case of the U.S. Forest Service. Gen. Tech. Rep. PNWGTR-757. Portland, OR: U.S. Department of Agriculture, Forest Service, Pacific Northwest Research Station. 78 p. This report examines the capacity of natural resource agencies to generate scientific knowledge and information for use by resource managers in planning and decisionmaking. This exploratory study focused on recreation in the U.S. Department of Agriculture, Forest Service. A semistructured, open-ended interview guide elicited insights from 58 managers and 28 researchers about recreation issues, information exchange, and research-management interactions. Data were coded and analyzed using Atlas.ti®, a qualitative analysis software program. Results indicate that recreation managers seek information to address user conflicts and manage diverse activities across sites and landscapes. Managers do not always turn to the research community when looking for scientific information and are uncertain about the proper channels for communication. Managers consult a variety of information sources and aggregate various types of scientific information for use in planning and management. Managers desire greater and more diverse interactions with researchers to promote knowledge exchange useful for addressing recreation problems. Barriers to interaction include organizational differences between management and research, researcher responsiveness, relevance of information to manager needs, and the lack of formal interaction opportunities. Several structural processes were suggested to facilitate opportunities for greater interaction and information exchange.

Monocyte phenotype and function were measured in whole blood sampled from a current cohort of human immunodeficiency virus (HIV)-infected individuals attending a large, metropolitan, university-affiliated hospital. There was no significant difference in the prevalence of CD16+ monocytes or the capacity of monocytes to ingest heat-killed Mycobacterium avium complex between these individuals and HIV-uninfected control subjects, regardless of viral load, current CD4+ T cell count, nadir CD4+ T cell count, or time since diagnosis of HIV infection. CD16+ monocyte prevalence was, however, elevated in patients not currently receiving antiretroviral therapy. We conclude that HIV type 1 infection in the setting of highly active antiretroviral therapy is associated with normal monocyte function and phenotype.

Stakeholder participation in collaborative watershed planning in Washington State was examined in three watershed planning unit cases in order to understand how stakeholders participate in collaborative watershed planning, the nature of plans developed, and stakeholder perceptions of plan implementation. Through document analysis and interviews, we found broad and inclusive representation of stakeholders in the planning processes, and that a majority of stakeholder comments were reflected in the final plans. Plans were of good quality and provide a strong foundation for implementation. Factors that facilitate plan implementation include the collaborative planning process itself, funding and community support, and leadership along with early and sustained involvement. Factors that hinder plan implementation include lack of adequate funding, various legal issues, and challenges in involving landowners in plan projects.

Conservation and natural resource literature and practice are permeated with calls for improving graduate education, with much attention paid to the mismatch between the disciplinary structure of most universities and the complexity of real world natural resource and conservation problems. At the university graduate level, students are developing advanced levels of expertise in a particular discipline or practice. Indeed, disciplinary education and research are crucial to providing fundamental knowledge related to environmental and conservation sciences. Yet, the environmental problems we face today are far too complicated to be approached with single-discipline or fragmented approaches.