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Diabetes mellitus (DM) involves metabolic changes that can negatively influence periodontal tissues, resulting in more prevalent and severe periodontitis and impaired bone formation. Occlusal trauma (OT) is an injury of the supportive periodontium that results in bone loss. It can be hypothesized that DM would increase bone loss after OT, mainly when associated with periodontitis. Thus, the aim of the present study is to evaluate the influence of DM on bone response in the furcation area of teeth subjected to OT in the presence, or absence, of experimental periodontitis (EP) in the rat model.Thirty-two male Wistar rats were assigned to four groups: 1) group 1 (G1): DM+OT+EP (n = 8); 2) group 2 (G2): DM+OT (n = 8); 3) group 3 (G3): OT+EP (n = 8); and 4) group 4 (G4): OT (n = 8). G1 and G2 received a single intraperitoneal injection of streptozotocin (STZ). After 10 days, G1 and G3 were subjected to EP by ligature placement. Fifteen days after the start of EP, OT was induced by the creation of a premature contact. The animals were euthanized 35 days after DM induction.DM enhanced bone loss in the presence of OT combined with EP, but did not increase bone loss in teeth subjected to OT alone. EP caused greater bone loss when associated with OT.Within the limits of this animal study, it can be concluded that DM enhances bone loss in the presence of occlusal trauma associated with EP.

Orientador: Enilson Antonio Sallum Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba Resumo: O objetivo do presente estudo foi avaliar a influência do diabetes mellitus (DM) sobre a reabsorção óssea alveolar ocorrida no trauma oclusal (TO) na presença e ausência da periodontite experimental (PE) em ratos. Para tanto, 32 ratos Wistar (Rattus Novergicus) adultos foram aleatoriamente distribuídos em 4 grupos: Grupo 1 (n=08): DM+TO+PE; Grupo 2 (n=08): DM+TO; Grupo 3 (n=08): não-diabético+TO+PE; Grupo 4 (n=08): não diabético+TO. O DM foi induzido nos animais do G1 e G2 pela injeção intraperitoneal de 60 mg/kg de estreptozotocina (STZ). Dez dias após a indução do DM, os animais do G1 foram induzidos à PE pela colocação da ligadura de fio de seda no sulco dos primeiros molares inferiores do lado esquerdo (lado teste); o mesmo foi feito nos animais não diabéticos (G3). O molar contralateral (direito) de todos os animais dos 4 grupos serviu como controle. O início da indução do TO, pela cimentação do fio ortodôntico na oclusal dos molares já submetidos à PE, se deu 15 dias após a colocação da ligadura (G1 e G3). No mesmo dia o TO também foi induzido nos grupos sem ligadura (G2 e G4) permanecendo por 10 dias até a data do sacrifício. A avaliação intra-grupo mostrou haver diferença estatística significante de perda óssea alveolar quando comparado os dentes do lado teste (TO) ao lado controle (p0,05) entre G2 (DM+TO) e G4 (TO). Dentro dos limites deste estudo pôde-se concluir que o diabetes mellitus agrava a perda óssea alveolar nos animais com trauma oclusal quando associado à periodontite experimental. Abstract: The aim of this study was to evaluate the diabetes mellitus (DM) effects on alveolar bone resorption induced by occlusal trauma (OT) with or without experimental periodontites (EP) in rats. Thirty-two adult male rats (Rattus Novergicus) were randomly assigned to one of four groups: Group1 (n=08): DM+OT+EP; Group 2 (n=08): DM+OT: Group 3 (n=08): OT+EP: Group 4 (n=08): OT. Experimental DM was created by intraperitoneal injection of 60 mg/kg body weight streptozotocin (STZ) in G1 and G2. Ten days after diabetes induction, the animals in G1 received a silk ligature in the sulcular area of their mandible left first molar (test side (OT) to induce EP; the same was performed with the non-diabetics animals (G3). The contralateral molar (right side) was left as a control in all of the groups. G1 and G3 were submitted to OT induction (by orthodontic wire bonding in the occlusal molar surface) after fifteen days period. At the same day, the OT was also inducted in the groups (02 and 04) without ligature. The animals were sacrificed 10 days later. The intragroup analysis revealed significant difference between teeth in the test side (OT) and control side (p