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2 results on '"Citrate carrier activity and expression"'

1. Hypothyroidism down-regulates mitochondrial citrate carrier activity and expression in rat liver

Author

Giudetti, Anna M., Leo, Monica, Siculella, Luisa, and Gnoni, Gabriele V.

Subjects
*FATTY acids, *HIGH performance liquid chromatography, *HYPOTHYROIDISM, *HYDROGEN-ion concentration
Abstract

Abstract: The effect of hypothyroidism on citrate carrier (CiC) activity has been investigated in rat-liver mitochondria. The rate of citrate transport was reduced by ∼50% in mitochondria from hypothyroid as compared with euthyroid rats. In parallel, a decrease in the rate of de novo fatty acid synthesis was observed in the cytosol of the former animals. Kinetic analysis of citrate transport revealed that only the V max was reduced by hypothyroidism, while K m was almost unaffected. Hypothyroidism increased the mitochondrial percentage of phosphatidylcholine while decreased that of phosphatidylethanolamine; an altered fatty acid pattern but no significant difference in the sum of saturated and unsaturated fatty acids as well as in the unsaturation index was observed. The CiC Arrhenius plot did not show appreciable difference between the two groups of rats. However, Western blot analysis associated with mRNA quantitation indicated that both protein level and mRNA accumulation of hepatic CiC were noticeably decreased in hypothyroid state. Therefore, a reduced content of the carrier protein can represent a plausible mechanism to explain the decline in the CiC activity observed in rat liver mitochondria of hypothyroid rats. [Copyright &y& Elsevier]

Published
2006
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2. Hypothyroidism down-regulates mitochondrial citrate carrier activity and expression in rat liver

Author

Monica Leo, Luisa Siculella, Anna Maria Giudetti, Gabriele V. Gnoni, Giudetti, Anna Maria, Leo, Monica, Siculella, Luisa, and Gnoni, Gabriele Vincenzo

Subjects
Male, endocrine system, medicine.medical_specialty, Thyroid Hormones, endocrine system diseases, Phospholipid, Down-Regulation, Mitochondrion, Biology, Citric Acid, chemistry.chemical_compound, Membrane Lipids, Hypothyroidism, Internal medicine, Phosphatidylcholine, Citrate carrier activity and expression, medicine, Animals, Rats, Wistar, Molecular Biology, Fatty acid synthesis, chemistry.chemical_classification, Phosphatidylethanolamine, Lipogenesi, Fatty acid, Cell Biology, Citrate transport, Mitochondria, Rats, Endocrinology, chemistry, Liver, Lipogenesis, Fatty Acid Synthases, Carrier Proteins, hormones, hormone substitutes, and hormone antagonists, Acetyl-CoA Carboxylase
Abstract

The effect of hypothyroidism on citrate carrier (CiC) activity has been investigated in rat-liver mitochondria. The rate of citrate transport was reduced by approximately 50% in mitochondria from hypothyroid as compared with euthyroid rats. In parallel, a decrease in the rate of de novo fatty acid synthesis was observed in the cytosol of the former animals. Kinetic analysis of citrate transport revealed that only the Vmax was reduced by hypothyroidism, while Km was almost unaffected. Hypothyroidism increased the mitochondrial percentage of phosphatidylcholine while decreased that of phosphatidylethanolamine; an altered fatty acid pattern but no significant difference in the sum of saturated and unsaturated fatty acids as well as in the unsaturation index was observed. The CiC Arrhenius plot did not show appreciable difference between the two groups of rats. However, Western blot analysis associated with mRNA quantitation indicated that both protein level and mRNA accumulation of hepatic CiC were noticeably decreased in hypothyroid state. Therefore, a reduced content of the carrier protein can represent a plausible mechanism to explain the decline in the CiC activity observed in rat liver mitochondria of hypothyroid rats.

Published
2006

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