The aim of this research was to evaluate the regeneration of bone tissue extracted at vertical and horizontal mandibular atrophies.Our research was based on 30 patients (15 men and 15 women, age range 35-70) who needed partial or total mandible regeneration, divided in 3 groups (in Vivo). In group I, 10 people were treated with blood Concentration Growth Factors, 5 men and 5 women (age 55±7.7, p<0.05) both smokers and non smokers, after that the flap was opened and then a sample was extracted by a mincing cutter (trephine burr) (Implatex; 3 mm inner diameter) for biopsy.; in group II, 10 people were treated with blood Concentration Growth Factors mixed with autologous bone, 5 men and 5 women (56.8 ± 8.4, p <0.05), smokers and non smokers with partial or total atrophy who needed implant prosthetic rehabilitation; in group III, patients were treated with Hydroxylapatite, Tricalcium phosphate and bCGF, 5 men and 5 women (56.8 ± 8.4, p<0.05), smokers and non smokers. After a 6th month follow-up all the biopsies received from the patients were immediately placed in 10% formalin (formaldehyde) and immersed into 0.5 M EDTA tamponade for demineralization. Extracted tissue after 6th month follow-up was examined histologically and immune-histo-chemically using Anti-RUNX, Anti-Alkaline Phosphatase, Anti-SPARC and Ki67. Each sample was colored with H/E. The sections were examined with a microscope (Nikon Eclipse E 600 microscope and Lucia G software for microscopic image analysis). Histological evaluation (HE) has demonstrated the complete bone formation at the patients of group II, partial ossification for the patients of group I, and moderate ossification with persisting H/A residues of the patients of group III. In an Immune-histo-chemical examination we obtained a different result in the group I, group II, group III (Table 1). The examination of each group showed that the best bone regeneration was detected in group II among the patients treated with the biomaterial of blood Concentration Growth Factors and autologous bone. During immune-histo-chemical examination we obtained significant differences between group I, group II, and group III in terms of the formation of a new bone (p⩽0.01). [ABSTRACT FROM AUTHOR]