1. Second harmonic generation signal from type I collagen fibers grown in vitro
- Author
-
Eric O. Potma, Raúl Rangel-Rojo, Israel Rocha-Mendoza, Cindy Grethel Fuentes-Corona, Jacob Licea-Rodriguez, and Rebecca Younger
- Subjects
0303 health sciences ,animal structures ,Materials science ,Scanning electron microscope ,Second-harmonic generation ,Optical Physics ,Materials Engineering ,Polarization (waves) ,01 natural sciences ,Article ,Atomic and Molecular Physics, and Optics ,In vitro ,Light scattering ,010309 optics ,03 medical and health sciences ,0103 physical sciences ,Composite material ,Anisotropy ,Order of magnitude ,Type I collagen ,030304 developmental biology ,Biotechnology - Abstract
We present a study of the optical second-order nonlinearity of type I collagen fibers grown in vitro via second harmonic generation (SHG) experiments and analyze the observed polarization-resolved SHG signal using previously reported SHG analytical expressions obtained for anisotropic tissue. Our results indicate that the effective second-order nonlinearity measured in the grown fibers is one order of magnitude lower than that of native collagen fibers. This is attributed to the formation of loose and dispersive fibrillar networks of thinner collagen fibrils that constitute the reassembled collagen fibers. This is confirmed by scanning electronic microscopy (SEM) imaging and the polarization dependence of the SHG signal. The measured values of the anisotropy parameter ρ of the reassembled collagen fibers are found to be similar to that obtained for native fibers on the relevant sub-µm scale.
- Published
- 2019