1. Fat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genes
- Author
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Paul Cordero, F Marzo, Fermín I. Milagro, José Alfredo Martínez, Javier Campión, Universidad Pública de Navarra. Departamento de Ciencias del Medio Natural, and Nafarroako Unibertsitate Publikoa. Natura Ingurunearen Zientziak Saila
- Subjects
Blood Glucose ,Male ,medicine.medical_specialty ,UCP1 ,Enzimas ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Plasmid delivery ,Citric Acid Cycle ,Glyoxylate cycle ,Expression ,Tail vein injection ,Carbohydrate metabolism ,Mice ,Endocrinology ,Malate synthase ,Internal medicine ,medicine ,Manipulation ,Short Paper ,Animals ,Obesity ,Ciclo de glioxilato ,lcsh:RC620-627 ,Biochemistry, medical ,biology ,Biochemistry (medical) ,Body Weight ,Malate Synthase ,Isocitrate lyase ,Carbohydrate ,Lyase ,Lipid Metabolism ,Isocitrate Lyase ,Rats ,Citric acid cycle ,Respiratory quotient ,Mice, Inbred C57BL ,lcsh:Nutritional diseases. Deficiency diseases ,Glucose ,Biochemistry ,Liver ,Glucosa ,biology.protein ,Carbohydrate Metabolism ,Mechanism - Abstract
The glyoxylate cycle, which is well characterized in higher plants and some microorganisms but not in vertebrates, is able to bypass the citric acid cycle to achieve fat-to-carbohydrate interconversion. In this context, the hydrodynamic transfer of two glyoxylate cycle enzymes, such as isocytrate lyase (ICL) and malate synthase (MS), could accomplish the shift of using fat for the synthesis of glucose. Therefore, 20 mice weighing 23.37 ± 0.96 g were hydrodinamically gene transferred by administering into the tail vein a bolus with ICL and MS. After 36 hours, body weight, plasma glucose, respiratory quotient and energy expenditure were measured. The respiratory quotient was increased by gene transfer, which suggests that a higher carbohydrate/lipid ratio is oxidized in such animals. This application could help, if adequate protocols are designed, to induce fat utilization for glucose synthesis, which might be eventually useful to reduce body fat depots in situations of obesity and diabetes.
- Published
- 2008