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11 results on '"Cicculli, Vincent"'

2. Lack of Evidence for Crimean--Congo Hemorrhagic Fever Virus in Ticks Collected from Animals, Corsica, France

Author

Cicculli, Vincent, Maitre, Apolline, Ayhan, Nazil, Mondoloni, Stevan, Paoli, Jean-Christophe, Vial, Laurence, de Lamballerie, Xavier N., Charrel, Remi, and Falchi, Alessandra

Subjects
Corsica -- Health aspects, Zoonoses -- Statistics -- Risk factors, Ticks -- Health aspects, Crimean hemorrhagic fever -- Statistics -- Risk factors, Animals as carriers of disease -- Health aspects, Health
Abstract

Crimean-Congo hemorrhagic fever (CCHF) is a tickborne zoonotic disease that is characterized by hemorrhagic fever and can progress from mild, nonspecific signs to a severe and fatal hemorrhagic disease. The [...]

Published
2022
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3. First detection of Jingmen tick virus in Corsica, France and development of a real time detection system for multiple tick-associated jingmenviruses

Author

Cicculli, Vincent, primary, Colmant, Agathe M. G., additional, Piorkowski, Geraldine, additional, Amaral, Rayane, additional, Maitre, Apolline, additional, Decarreaux, Dorine, additional, Thirion, Laurence, additional, Moureau, Gregory, additional, Falchi, Alessandra, additional, de Lamballerie, Xavier, additional, Charrel, Remi N., additional, and Ayhan, Nazli, additional

Published
2024
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6. Rickettsial pathogens drive microbiota assembly in Hyalomma marginatum and Rhipicephalus bursa ticks.

Author

Maitre, Apolline, Wu‐Chuang, Alejandra, Mateos‐Hernández, Lourdes, Piloto‐Sardiñas, Elianne, Foucault‐Simonin, Angélique, Cicculli, Vincent, Moutailler, Sara, Paoli, Jean‐Christophe, Falchi, Alessandra, Obregón, Dasiel, and Cabezas‐Cruz, Alejandro

Subjects
RICKETTSIA, TICKS, HYALOMMA, RHIPICEPHALUS, PATHOGENIC microorganisms, MICROBIAL communities, MICROORGANISMS
Abstract

Most tick‐borne pathogens (TBPs) are secondarily acquired by ticks during feeding on infected hosts, which imposes 'priority effect' constraints, as arrival order influences the establishment of new species in a microbial community. Here we tested whether once acquired, TBPs contribute to bacterial microbiota functioning by increasing community stability. For this, we used Hyalomma marginatum and Rhipicephalus bursa ticks collected from cattle in different locations of Corsica and combined 16S rRNA amplicon sequencing and co‐occurrence network analysis, with high‐throughput pathogen detection, and in silico removal of nodes to test for impact of rickettsial pathogens on network properties. Despite its low centrality, Rickettsia showed preferential connections in the networks, notably with a keystone taxon in H. marginatum, suggesting facilitation of Rickettsia colonisation by the keystone taxon. In addition, conserved patterns of community assembly in both tick species were affected by Rickettsia removal, suggesting that privileged connections of Rickettsia in the networks make this taxon a driver of community assembly. However, Rickettsia removal had minor impact on the conserved 'core bacterial microbiota' of H. marginatum and R. bursa. Interestingly, networks of the two tick species with Rickettsia have similar node centrality distribution, a property that is lost after Rickettsia removal, suggesting that this taxon drives specific hierarchical interactions between bacterial microbes in the microbiota. The study indicates that tick‐borne Rickettsia play a significant role in the tick bacterial microbiota, despite their low centrality. These bacteria are influential and contribute to the conservation of the 'core bacterial microbiota' while also promoting community stability. [ABSTRACT FROM AUTHOR]

Published
2023
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7. Molecular detection of parapoxvirus in Ixodidae ticks collected from cattle in Corsica, France

Author

Cicculli, Vincent, Ayhan, Nazli, Luciani, Léa, Pezzi, Laura, Maitre, Apolline, Decarreaux, Dorine, De Lamballerie, Xavier, Paoli, Jean-Christophe, Vial, Laurence, Charrel, Rémi, Falchi, Alessandra, Cicculli, Vincent, Ayhan, Nazli, Luciani, Léa, Pezzi, Laura, Maitre, Apolline, Decarreaux, Dorine, De Lamballerie, Xavier, Paoli, Jean-Christophe, Vial, Laurence, Charrel, Rémi, and Falchi, Alessandra

Abstract

Background: Several viruses belonging to the family Poxviridae can cause infections in humans and animals. In Corsica, livestock farming (sheep, goats, pigs, and cattle) is mainly mixed, leading to important interactions between livestock, wildlife, and human populations. This could facilitate the circulation of zoonotic diseases, and makes Corsica a good example for studies of tick-borne diseases. Objectives: To gain understanding on the circulation of poxviruses in Corsica, we investigated their presence in tick species collected from cattle, sheep, horses, and wild boar, and characterized them through molecular techniques. Methods: Ticks were tested using specific primers targeting conserved regions of sequences corresponding to two genera: parapoxvirus and orthopoxvirus. Results: A total of 3555 ticks were collected from 1549 different animals (687 cattle, 538 horses, 106 sheep, and 218 wild boars). They were tested for the presence of parapoxvirus DNA on one hand and orthopoxvirus DNA on the other hand using Pangeneric real-time TaqMan assays. Orthopoxvirus DNA was detected in none of the 3555 ticks. Parapoxvirus DNA was detected in 6.6% (36/544) of ticks collected from 23 cows from 20 farms. The remaining 3011 ticks collected from horses, wild boars, and sheep were negative. The infection rate in cow ticks was 8.0% (12/148) in 2018 and 6.0% (24/396) in 2019 (p = 0.57). Parapoxvirus DNA was detected in 8.5% (5/59) of Hyalomma scupense pools, 8.2% (15/183) of Hyalomma marginatum pools, and 6.7% (16/240) of Rhipicephalus bursa pools (p = 0.73). We successfully amplified and sequenced 19.4% (7/36) of the positive samples which all corresponded to pseudocowpox virus. Conclusions: Obviously, further studies are needed to investigate the zoonotic potential of pseudocowpox virus and its importance for animals and public health.

Published
2022

11. First detection of Jingmen tick virus in Corsica, France and development of a real time detection system for multiple tick-associated jingmenviruses.

Author

Cicculli V, Colmant AMG, Piorkowski G, Amaral R, Maitre A, Decarreaux D, Thirion L, Moureau G, Falchi A, de Lamballerie X, Charrel RN, and Ayhan N

Abstract

Jingmen tick virus (JMTV) is a recently discovered segmented RNA virus, closely related to flaviviruses. It was identified for the first time in 2014, in China and subsequently in Brazil. Following this discovery, JMTV-related sequences have been identified in arthropods, vertebrates (including humans), plants, fungus and environmental samples from Asia, America, Africa, Europe and Oceania. Several studies suggest an association between these segmented flavi-like viruses, termed jingmenviruses, and febrile illness in humans. The development of rapid diagnostic assays for these viruses is therefore crucial to be prepared for a potential epidemic, for the early detection of these viruses via vector surveillance or hospital diagnosis. In this study, we designed a RT-qPCR assay to detect tick-associated jingmenviruses, validated it and tested its range and limit of detection with six tick-associated jingmenviruses using in vitro transcripts. Then we screened ticks collected in Corsica (France) from different livestock species, in order to determine the distribution of these viruses on the island. In total, 6,269 ticks from eight species were collected from 763 cattle, 538 horses, 106 sheep and 218 wild boars and grouped in 1,715 pools. We report the first detection of JMTV in Corsica, in Rhipicephalus bursa, Hyalomma marginatum and R. sanguineus ticks collected from cattle and sheep. The highest prevalence was found in the Rhipicephalus genus. The complete genome of a Corsican JMTV was obtained from a pool of Rhipicephalus bursa ticks and shares between 94.7% and 95.1% nucleotide identity with a JMTV sequence corresponding to a human patient in Kosovo and groups phylogenetically with European JMTV strains. These results show that a Mediterranean island such as Corsica could act as a sentinel zone for future epidemics., Competing Interests: Competing interests All authors declare no financial or non-financial competing interests.

Published
2024
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