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3. Specificity of Human T Lymphocytes Expressing a γ/δT Cell Antigen Receptor. Recognition of a Polymorphic Determinant of HLA Class I Molecules by a γ/δ+ Clone

Author

Ciccone, E., Viale, O., Pende, Daniela, Malnati, M., Moretta, A., Moretta, L., Compans, R. W., editor, Cooper, M., editor, Koprowski, H., editor, McConnell, I., editor, Melchers, F., editor, Nussenzweig, V., editor, Oldstone, M., editor, Olsnes, S., editor, Potter, M., editor, Saedler, H., editor, Vogt, P. K., editor, Wagner, H., editor, Wilson, I., editor, Pfeffer, Klaus, editor, Heeg, Klaus, editor, Wagner, Hermann, editor, and Riethmüller, Gert, editor

Published
1991
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7. Studio multidisciplinare per la valutazione delle deformazioni del suolo connesse alle attività di produzione di idrocarburi in aree dell'offshore emiliano romagnolo finalizzato allo sviluppo di un modello per il monitoraggio integrato

Author

I. Antoncecchi, G. Rossi, F. Ciccone, E. Carminati, A. Morelli, G. Pezzo, P. Macini, S. Gandolfi, R. Lanari, M. Manzo and F. Moia, F. Verga, L. Perini, P. Severi, L. Petracchini, F. Terlizzese

Subjects
offshore, modello integrato, deformazione del suolo, idrocarburi
Abstract

La DGS UNMIG ha promosso, nell'ambito della rete di ricerca CLYPEA "il network per la sicurezza offshore", uno studio multidisciplinare per la valutazione delle deformazioni del suolo connesse ad attività di produzione di idrocarburi in aree marino-costiere dell'offshore emiliano-romagnolo finalizzato allo sviluppo di un modello per il monitoraggio integrato. L'iniziativa nasce nel 2016 a seguito dell'accordo siglato ad Ottobre tra il Ministero Sviluppo Economico e la Regione Emilia Romagna (accordo offshore), con l'intento di verificare una possibile estensione a mare degli "Indirizzi e Linee Guida per il monitoraggio della sismicità, delle deformazioni del suolo e delle pressioni di poro" (ILG, 2014). Il progetto "subsidenza" si pone l'obiettivo di affrontare il tema del monitoraggio e controllo della subsidenza in mare che, già da diversi anni, rappresenta un elemento chiave per il mantenimento di elevati standard di sicurezza nelle operazioni di produzione di idrocarburi integrando le competenze di 6 enti specializzati (tra università e centri di ricerca). Di seguito si riportano i diversi contributi alle attività che contribuiranno al raggiungimento degli obiettivi del progetto.

Published
2018

10. Anatomia Umana

Author

Arcari, Ml, Bandiera, P, Benagiano, V, Cavalletti, G, Ciccone, E, De Angelis MG, and D'Agata, VELIA MARIA

Published
2017

11. Molecules that inhibit T-cell functions: cytochemical localization and shuttling

Author

Santoro, G., Giuseppe Pio ANASTASI, Saverino, D., Puri, C., Zarcone, D., Tacchetti, C., Ciccone, E., Grossi, C. E., Santoro, G., Anastasi, G., Saverino, D., Puri, C., Zarcone, D., Tacchetti, Carlo, Ciccone, E., and Grossi, C. E.

Subjects
lcsh:Biology (General), lcsh:QH301-705.5
Abstract

Adaptive immune responses to antigens are mediated by specific receptors expressed on B cells (BCR’s) and T cells (TCR’s). Effector cells and memory cells are produced following a proliferative wave that accounts for clonal expansion. If not down-regulated, clonal expansion might lead to uncontrolled lymphoproliferation that would be harmful for the organism. Several mechanisms that account for the down-sizing of activated lymphocyte clones are briefly reviewed here. We next consider in detail one such mechanism that deals with the functional characterization and the immunocytochemical localization of two T-cell inhibitory molecules, namely the Cytotoxic T Lymphocyte Antigen-4 (CTLA-4) and the HP-F1 antigen, both present in all T lymphocytes . CTLA-4 and HP-F1 inhibit CD4+ T-helper cell proliferation and the lytic ability of CD8+ T-cytotoxic cells in non-specific and in antigen-specific cytolytic assays. Interestingly, a clonal distribution exists as for the ability of CTLA-4 and HP-F1 to inhibit T-cell functions. In resting and activated T cells, both molecules are largely confined in the endosomal compartment, as shown by immunofluorescence analyses. However, upon interaction of T cells with Antigen-Presenting Cells (APC’s) or with target cells that must be killed, CTLA-4 molecules are transported to the plasma membrane, at the site of cell-to-cell contact where, following interaction with ligands, they trigger inhibitory signals.

Published
2009

12. Speech-language pathology findings in patients with mouth breathing: Multidisciplinary diagnosis according to etiology

Author

Haddad L, de Oliveira Lr, Junqueira P, Ciccone E, Rizzo Mc, and Marchesan Iq

Subjects
medicine.medical_specialty, Pathology, medicine.diagnostic_test, business.industry, Mouth breathing, General Medicine, Facial muscles, medicine.anatomical_structure, Otorhinolaryngology, Swallowing, Tongue, medicine, Etiology, medicine.symptom, Audiometry, business, Tongue Habits
Abstract

The purpose of this study was to identify and compare the results of the findings from speech-language pathology evaluations for orofacial function including tongue and lip rest postures, tonus, articulation and speech, voice and language, chewing, and deglutition in children who had a history of mouth breathing. The diagnoses for mouth breathing included: allergic rhinitis, adenoidal hypertrophy, allergic rhinitis with adenoidal hypertrophy; and/or functional mouth breathing. This study was conducted with on 414 subjects of both genders, from 2 to 16-years old. A team consisting of 3 speech-language pathologists, 1 pediatrician, 1allergist, and 1 otolaryngologist, evaluated the patients. Multidisciplinary clinical examinations were carried out (complete blood counting, X-rays, nasofibroscopy, audiometry). The two most commonly found etiologies were allergic rhinitis, followed by functional mouth breathing. Of the 414 patients in the study, 346 received a speech-language pathology evaluation. The most prevalent finding in this group of 346 subjects was the presence of orofacial myofunctional disorders. The most frequently orofacial myofunctional disorder identified in these subjects who also presented mouth breathing included: habitual open lips rest posture, low and forward tongue rest posture and lack of adequate muscle tone. There were also no statistically significant relationships identified between etiology and speech-language diagnosis. Therefore, the specific type of etiology of mouth breathing does not appear to contribute to the presence, type, or number of speech-language findings which may result from mouth breathing behavior.

Published
2010

13. Progetto SOPHY: Studio osservazionale sul pH vaginale e sullo stile di vita della donna nelle diverse età e condizioni fisiopatologiche. Parte I

Author

Guaschino, S., Benvenuti, C., Agnello, A., Agnolotti, M., Agostinelli, D., Agrifoglio, V., Albani, F., Alesi, L., Amadori, A., Andresini, R., Anelli, R., Antoniello, M., Arcadia, P., Arduino, S., Atlante, M., Bagnara, M., Balestrucci, G., Barbini, V., Barletta, F., Bassan, M., Bechini, F., Belsan, R., Benatti, G., Beretta, R., Bernardi, F., Bersani, R., Bertocchi, L., Bianchi, M. S., Bianchi, S., Biello, A., Bolelli, E., Bonaccorsi, G., Bonauguri, F., Bondesan, A., Bordignon, D., Bozzo, G., Brambilla, T., Brizio, A. M., Brusa, C., Businco, F., Cadario Preti, E., Calanchini, C., Capodieci, C., Cardinale, A., Carnio, P., Carnuccio, G., Casa, A., Castagna, P., Cazzavacca, R., Chiodi, A., Ciancio, G., Ciccone, E., Cicotti, M. P., Cino, S., Cirri, R., Citterio, S., Colla, F., Colombi, C., Conti, C. S., Corazza, G., Crana, F., Creanza, V., Crisafulli, M. L., Cristiani, P., Cugini, A. M., Curto, G., Cutuli, A., Dal Bò, R., Damone, R., De Majo, D., De Nuzzo, M. A., De Rosa, E., De Valle Vietti, G., Defazio, D. D., Delli Ponti, E. C., Di Bari, I., Di Biase, R., Di Cosmo, E., Di Giovinazzo, L., Di Natale, R., Di Pietro, F., Esposito, E., Fabbrizi, L., Faggionato, I., Fanti, S., Favi, O., Fazio Pellacchio, C., Ferraina, F., Ferrari, D., Ferrentino, A., Fiscella, A., Fischetti, A., Florio, V., Forcella, G., Franzolini, P., Furani, S., Fuschini, G., Gallo, G., Gammi, L., Geda, O., Gianfranceschi, C., Gigante, A., Giolito, M. R., Giordano, A., Giovagnorio, P., Giuliani, L., Gostinicchi, P., Gozeni, M., Guadalupi, E., Guagliarlo, M., Guastaferro, L., Gubbiotti, R., Guernieri, A., Guidi, A., Guzzinati, N., Iannacci, M. C., Iannelli, S., Infante, F. E., Insacco, P., Jannacone, D., Lalanne, A., Lanfranchi, A., Latella, R., Lazzarin, L., Lefosse, M. G., Lemme, E., Lepadatu, C., Levanti, S., Liberio, M. D., Linsalata, I., Lombardi, P., Luchi, C., Lucianetti, M., Luciano, V., Maccarini, U., Maggio, I., Maggiorelli, M., Maietta, A., Mancini, A. C., Mancini, L., Mancini, F., Manni, M. U., Manuzio, D., Manzan, L., Marasca, O., Marchi, M., Marcozzi, S., Maresi, M. P., Mariatti, M., Martinelli, P., Masi, P., Masini, M., Massa, E., Matanã², S., Mattioli, V., Mazzoli, F., Mazzucato, R., Medori, A., Melappioni, S., Mendolicchio, S., Meroni, S., Miliffi, L., Minorini, D., Mirra, P., Mori, R., Moroni, S., Mossetti, M., Motta, N., Mucci, M., Munizza, W., Mura, M., Musconi, M., Mussida, M., Nanni, C., Nardi, E., Nardi, M., Nesi, D., Nirta, A., Nozza, M., Oliva, N., Oliverio, C., Onofri, M. C., Paduano, F., Palombino, K., Papadia, L. S., Parvaneh, H., Pascazio, F., Pasini, T., Pasini, A., Paticchio, M. R., Pellegrinotti, A., Pensabene, I., Perilli, M. L., Perrini, G., Perugini, A., Pessina, M., Petracchi, M., Pieracci, R., Pignata, M., Pisaturo, G., Po, E., Poggi, M. G., Poli, M., Polpatelli, L., Pone, A., Ponticelli, R., Potenza, M. T., Previdi, A. M., Preziuso, M., Quagliarini, V., Quaranta, M., Quattrocchi, G., Ragusa, S., Rainã², M. I., Regge, G. M., Rizzo, S., Roberti, P., Romano, G., Romano, D., Rossi, M., Ruggeri, C., Ruggiero, G., Russo, C., Russotto, C. M., Salmi, P., Salvestroni, C., Salviato, M. G., Sangiorgi, B., Santandrea, V., Santomauro, S., Santoni, S., Sarica, F., Savoca, S., Scandellari, E., Scarpellini, M., Sciarra, M. N., Scibilia, M. R., Scopacasa, P., Serafini, T., Setaccioli, M., Settembrini, L., Sganga, E., Simionato, S., Sommacampagna, P., Spadaro, F., Spettoli, D. A., Speziale, M., Spiga, A. M., Stampone, R., Stefanidou, M., Stefanutti, B., Stolfi, G., Strazzari, G., Tabanelli, S., Tafuri, A., Tamburini, M., Tampucci, S., Tarantini, P., Tempesta, N., Testoni, N., Testori, P., Tomba, D., Toscano, M., Trapassi, L., Tripodi, M., Vadalã , A., Valentino, V., Valieri, M., Valsecchi, L., Vassena, L., Ventimiglia, L., Vicentini, M. T., Volonterio, A. M., Votano, S., Wittemberg, L., Zangara, C., Zecchi, R., ORIGONI, MASSIMO, Guaschino, S., Benvenuti, C., Agnello, A., Agnolotti, M., Agostinelli, D., Agrifoglio, V., Albani, F., Alesi, L., Amadori, A., Andresini, R., Anelli, R., Antoniello, M., Arcadia, P., Arduino, S., Atlante, M., Bagnara, M., Balestrucci, G., Barbini, V., Barletta, F., Bassan, M., Bechini, F., Belsan, R., Benatti, G., Beretta, R., Bernardi, F., Bersani, R., Bertocchi, L., Bianchi, M. S., Bianchi, S., Biello, A., Bolelli, E., Bonaccorsi, G., Bonauguri, F., Bondesan, A., Bordignon, D., Bozzo, G., Brambilla, T., Brizio, A. M., Brusa, C., Businco, F., Cadario Preti, E., Calanchini, C., Capodieci, C., Cardinale, A., Carnio, P., Carnuccio, G., Casa, A., Castagna, P., Cazzavacca, R., Chiodi, A., Ciancio, G., Ciccone, E., Cicotti, M. P., Cino, S., Cirri, R., Citterio, S., Colla, F., Colombi, C., Conti, C. S., Corazza, G., Crana, F., Creanza, V., Crisafulli, M. L., Cristiani, P., Cugini, A. M., Curto, G., Cutuli, A., Dal Bò, R., Damone, R., De Majo, D., De Nuzzo, M. A., De Rosa, E., De Valle Vietti, G., Defazio, D. D., Delli Ponti, E. C., Di Bari, I., Di Biase, R., Di Cosmo, E., Di Giovinazzo, L., Di Natale, R., Di Pietro, F., Esposito, E., Fabbrizi, L., Faggionato, I., Fanti, S., Favi, O., Fazio Pellacchio, C., Ferraina, F., Ferrari, D., Ferrentino, A., Fiscella, A., Fischetti, A., Florio, V., Forcella, G., Franzolini, P., Furani, S., Fuschini, G., Gallo, G., Gammi, L., Geda, O., Gianfranceschi, C., Gigante, A., Giolito, M. R., Giordano, A., Giovagnorio, P., Giuliani, L., Gostinicchi, P., Gozeni, M., Guadalupi, E., Guagliarlo, M., Guastaferro, L., Gubbiotti, R., Guernieri, A., Guidi, A., Guzzinati, N., Iannacci, M. C., Iannelli, S., Infante, F. E., Insacco, P., Jannacone, D., Lalanne, A., Lanfranchi, A., Latella, R., Lazzarin, L., Lefosse, M. G., Lemme, E., Lepadatu, C., Levanti, S., Liberio, M. D., Linsalata, I., Lombardi, P., Luchi, C., Lucianetti, M., Luciano, V., Maccarini, U., Maggio, I., Maggiorelli, M., Maietta, A., Mancini, A. C., Mancini, L., Mancini, F., Manni, M. U., Manuzio, D., Manzan, L., Marasca, O., Marchi, M., Marcozzi, S., Maresi, M. P., Mariatti, M., Martinelli, P., Masi, P., Masini, M., Massa, E., Matanã², S., Mattioli, V., Mazzoli, F., Mazzucato, R., Medori, A., Melappioni, S., Mendolicchio, S., Meroni, S., Miliffi, L., Minorini, D., Mirra, P., Mori, R., Moroni, S., Mossetti, M., Motta, N., Mucci, M., Munizza, W., Mura, M., Musconi, M., Mussida, M., Nanni, C., Nardi, E., Nardi, M., Nesi, D., Nirta, A., Nozza, M., Oliva, N., Oliverio, C., Onofri, M. C., Origoni, Massimo, Paduano, F., Palombino, K., Papadia, L. S., Parvaneh, H., Pascazio, F., Pasini, T., Pasini, A., Paticchio, M. R., Pellegrinotti, A., Pensabene, I., Perilli, M. L., Perrini, G., Perugini, A., Pessina, M., Petracchi, M., Pieracci, R., Pignata, M., Pisaturo, G., Po, E., Poggi, M. G., Poli, M., Polpatelli, L., Pone, A., Ponticelli, R., Potenza, M. T., Previdi, A. M., Preziuso, M., Quagliarini, V., Quaranta, M., Quattrocchi, G., Ragusa, S., Rainã², M. I., Regge, G. M., Rizzo, S., Roberti, P., Romano, G., Romano, D., Rossi, M., Ruggeri, C., Ruggiero, G., Russo, C., Russotto, C. M., Salmi, P., Salvestroni, C., Salviato, M. G., Sangiorgi, B., Santandrea, V., Santomauro, S., Santoni, S., Sarica, F., Savoca, S., Scandellari, E., Scarpellini, M., Sciarra, M. N., Scibilia, M. R., Scopacasa, P., Serafini, T., Setaccioli, M., Settembrini, L., Sganga, E., Simionato, S., Sommacampagna, P., Spadaro, F., Spettoli, D. A., Speziale, M., Spiga, A. M., Stampone, R., Stefanidou, M., Stefanutti, B., Stolfi, G., Strazzari, G., Tabanelli, S., Tafuri, A., Tamburini, M., Tampucci, S., Tarantini, P., Tempesta, N., Testoni, N., Testori, P., Tomba, D., Toscano, M., Trapassi, L., Tripodi, M., Vadalã , A., Valentino, V., Valieri, M., Valsecchi, L., Vassena, L., Ventimiglia, L., Vicentini, M. T., Volonterio, A. M., Votano, S., Wittemberg, L., Zangara, C., and Zecchi, R.

Subjects
Sexual behavior, Vagina, secretion, Vaginosis, bacterial, Obstetrics and Gynecology, Candida
Abstract

Aim. The importance of vaginal pH and vaginal flora in maintaining a well-balanced vaginal ecosystem is well known and has been widely described. However, no systematic nationwide studies have been carried out concerning the correlation between vaginal pH, life style and different physiopathological conditions in women of different ages. Methods. SOPHY (Study on pH and Hygiene) collected data concerning the lifestyle, vaginal pH, and the presence of symptoms, stratified into different subgroups (prepuberal, fertile, pregnancy, postpartum, premenopause and menopause) in a representative sample of the Italian gynecological population (264 gynaecologists for a total of 2 641 women) with the aid of a specific Internet site for data entry. Results. A more acid vaginal pH was related to a better satisfactory sexual activity and to more healthy genital condition. A positive relationship was detected between education level and good perception of sexuality. Certain clothing habits and a higher frequency of candidiasis and bacterial vaginosis was shown. Conclusion. SOPHY revealed some interesting correlations between clothing and the frequency of candidiasis and bacterial vaginosis, between vaginal pH and satisfactory sexual activity, and between education and sexuality. SOPHY had a considerable educational impact, leading the physicians and women to consider vaginal pH as an important aspect of everyday life.

Published
2008

14. Specific recognition of the viral protein UL18 by CD85/LIR-1/ITL2 on T cells mediates the non-MHC-restricted lysis of human cytomegalovirus infected cells

Author

Saverino D., Ghiotto, F., Merlo, A., Bruno S., Battini L., Occhino M., Maffei M., Tenca C., PILERI, STEFANO, Baldi L., Fabbi M., Bachi A., De Santanna A., Grossi C., Ciccone E., Saverino D., Ghiotto, F., Merlo, A., Bruno S., Battini L., Occhino M., Maffei M., Tenca C., Pileri S., Baldi L., Fabbi M., Bachi A., De Santanna A., Grossi C., and Ciccone E.

Published
2004

16. Cytokine gene expression and T-cell proliferative responses in lymph node mononuclear cells from children with early stage human immunodeficiency virus infection

Author

Airoldi, I., Saverino, D., Favre, A., Ghiotto, F., Tacchetti, C., Facchetti, P., Piatti, G., Giuseppina Li Pira, Fenoglio, D., Duse, M., Ciccone, E., Manca, F., Plebani, A., Grossi, C. E., Pistoia, V., Airoldi, I, Saverino, D, Favre, A, Ghiotto, F, Tacchetti, Carlo, Facchetti, P, Piatti, G, Li Pira, G, Fenoglio, D, Duse, M, Ciccone, E, Manca, F, Plebani, A, Grossi, C. E., and Pistoia, V.

Subjects
Male, T-Lymphocytes, Gene Expression, Infant, HIV Infections, Lymphocyte Activation, HIV infection, cytokine gene espression, T cell function, Child, Preschool, Leukocytes, Mononuclear, Cytokines, Humans, RNA, Female, Lymph Nodes, Child
Abstract

Background and Objectives. The immunologic events taking place in secondary lymphoid tissue from children with early stage human immunodefi- ciency virus (HIV) infection are poorly understood. The aim of this study was to investigate cytokine gene expression and proliferative responses in lymph node (LN) biopsies from five children with ear- ly stage HIV infection, in the context of LN morphol- ogy and viral load.Design and Methods. The design of the study was approved by the local Ethical Committee. Cytokine gene expression was studied in LN biopsies and in paired peripheral blood (PB) samples from HIV- infected children by reverse transcriptase-poly- merase chain reaction. T-cell proliferation was assessed by 3H-thymidine incorporation. Viral bur- den in germinal centers was assessed by video den- sitometric analysis following immunohistochemical staining for HIV p24.Results. Interleukin (IL)-2, IL-4 and IL-5 mRNA were not detected in any LN or PB sample from HIV-infect- ed children. Interferon (IFN)-γ mRNA was found only in CD8+ cells. IL-12 p35, IL-10, transforming growth factor-(TGF)-β1, regulated on activation normal T- cell expressed and secreted (RANTES), macrophage inflammatory protein (MIP)-1α, MIP-1β and IL-16 transcripts were detected in all samples. Prolifera- tion of LN and PB mononuclear cells to polyclonal mitogens and soluble (recall and HIV-related) anti- gens was impaired as compared with the responses in a group of age-matched healthy controls.Interpretation and Conclusions. Changes in cytokine gene expression and T-cell proliferative responses are already detectable in lymph nodes from HIV- infected children at an early stage of disease.

Published
2000

17. Involvement of HLA class I alleles in natural killer (NK) cell-specific functions: expression of HLA-Cw3 confers selective protection from lysis by alloreactive NK clones displaying a defined specificity (specificity 2)

Author

Ciccone, E., Pende, D., Viale, O., Than, A., Di Donato, C., Orengo, A. M., Biassoni, R., Verdiani, S., Amoroso, A., Moretta, A., and Moretta, L.

Subjects
Cytotoxicity, Immunologic, Male, Herpesvirus 4, Human, medicine.drug_class, Immunology, Molecular Sequence Data, Gene Expression, Genes, MHC Class I, Human leukocyte antigen, HLA-C Antigens, Major histocompatibility complex, Monoclonal antibody, Lymphocyte Activation, Polymerase Chain Reaction, Natural killer cell, medicine, Immunology and Allergy, Humans, Alleles, Cells, Cultured, Cell Line, Transformed, Genetics, B-Lymphocytes, biology, Base Sequence, Antibodies, Monoclonal, Genetic Variation, Transfection, Articles, Phenotype, Molecular biology, Clone Cells, Killer Cells, Natural, Cytolysis, medicine.anatomical_structure, Haplotypes, Oligodeoxyribonucleotides, Cell culture, biology.protein, Female
Abstract

This study was designed to identify the target molecules of the natural killer (NK) cell-mediated recognition of normal allogeneic target cells. As previously shown, the gene(s) governing the first NK-defined allospecificity (specificity 1) were found to be localized in the major histocompatibility complex region between BF gene and HLA-A. In addition, the analysis of a previously described family revealed that a donor (donor 81) was heterozygous for three distinct NK-defined allospecificities (specificities 1, 2, and 5). HLA variants were derived from the B-Epstein-Barr virus cell line of donor 81 by gamma irradiation followed by negative selection using monoclonal antibodies specific for the appropriate HLA allele. Several variants were derived that lacked one or more class I antigen expressions. These variants were analyzed for the susceptibility to lysis by NK clones recognizing different allospecificities. The loss of HLA-A did not modify the phenotype (i.e., "resistance to lysis"). On the other hand, a variant lacking expression of all class I antigens became susceptible to lysis by all alloreactive clones. Variants characterized by the selective loss of class I antigens coded for by the maternal chromosome became susceptible to lysis by anti-2-specific clones. Conversely, variants selectively lacking class I antigens coded for by paternal chromosome became susceptible to lysis by anti-1 and anti-5 clones (but not by anti-2 clones). Since the Cw3 allele was lost in the variant that acquired susceptibility to lysis by anti-2 clones and, in informative families, it was found to cosegregate with the character "resistance to lysis" by anti-2 clones, we analyzed whether Cw3 could represent the element conferring selective resistance to lysis by anti-2 clones. To this end, murine P815 cells transfected with HLA Cw3 (or with other HLA class I genes) were used as target cells in a cytolytic assay in which effector cells were represented by alloreactive NK clones directed against different specificities. Anti-2-specific clones efficiently lysed untransfected or A2-, A3-, and A24-transfected P815 cells, while they failed to lyse Cw3-transfected cells. NK clones recognizing specificities other than specificity 2 lysed untransfected or Cw3-transfected cells. Thus, the loss of Cw3 resulted in the de novo appearance of susceptibility to lysis, and transfection of the HLA-negative P815 cells with Cw3 resulted in resistance to lysis by anti-2 clones. Therefore, we can infer that Cw3 expression on (both human and murine) target cells confers selective protection from lysis mediated by anti-2 NK clones.

Published
1992

18. Susceptibility or resistance to lysis by alloreactive natural killer cells is governed by a gene in the human major histocompatibility complex between BF and HLA-B

Author

Ciccone, E., Colonna, M., Viale, O., Pende, D., Di Donato, C., Reinharz, D., Amoroso, A., Jeannet, M., Guardiola, J., Moretta, A., Spies, T., Strominger, J., and Moretta, L.

Subjects
Antigens, Differentiation, T-Lymphocyte, Cytotoxicity, Immunologic, Male, CD3 Complex, Cytotoxicity, Receptors, Fc, Histocompatibility Testing, Antigens, Differentiation, CD4 Antigens, Chromosome Mapping, Female, HLA-B Antigens, Haplotypes, Humans, Killer Cells, Natural, Lymphocytes, Multigene Family, Pedigree, Polymorphism, Restriction Fragment Length, Receptors, Antigen, T-Cell, Receptors, IgG, Major Histocompatibility Complex, Immunologic, Receptors, Killer Cells, Genetics, Multidisciplinary, Fc, biology, HLA-B, medicine.anatomical_structure, Differentiation, Antigen, Natural, Research Article, IgG, Locus (genetics), Human leukocyte antigen, Major histocompatibility complex, Natural killer cell, medicine, Antigens, Polymorphism, Haplotype, T-Cell, Restriction Fragment Length, T-Lymphocyte, biology.protein
Abstract

The specificity recognized on normal allogeneic cells by a given alloreactive (1-anti-A) natural killer clone is controlled by a gene locus termed EC1. Because the EC1 locus was previously shown to be located on chromosome 6, families characterized by a recombinant major histocompatibility complex haplotype were analyzed to map this locus more precisely. The breakpoint of recombination was studied by standard HLA typing, complement typing, and restriction fragment length polymorphism analysis of a series of genes located between the complement cluster genes and HLA-B within the major histocompatibility complex region. Three of 10 families analyzed were informative. From the data obtained, the EC1 locus maps between BF and HLA-B and presumably is one of the normal genes recently described in this region.

Published
1990

37. A novel pleckstrin homology-related gene family defined by Ipl/Tssc3, TDAG51, and Tih1 : tissue-specific expression, chromosomal location, and parental imprinting.

Author

Frank, D, Mendelsohn, C L, Ciccone, E, Svensson, K, Ohlsson, R, Tycko, B, Frank, D, Mendelsohn, C L, Ciccone, E, Svensson, K, Ohlsson, R, and Tycko, B

Abstract

We previously described a gene, Ipl (Tssc3), that is expressed selectively from the maternal allele in placenta, yolk sac, and fetal liver and that maps within the imprinted domain of mouse distal Chromosome (Chr) 7/human Chr 11p15.5 (Hum Mol Genet 6, 2021, 1997). Ipl is similar to TDAG51, a gene that is involved in FAS/CD95 expression. Here we describe another gene, Tih1 (TDAG/Ipl homologue 1), with equivalent sequence similarity to Ipl. Structural prediction indicates that the products of these three genes share a central motif resembling a pleckstrin-homology (PH) domain, and TIH1 protein has weak sequence similarity to the PH-domain protein SEC7/CYTOHESIN. Like Ipl, Tih1 is a small gene with a single small intron. Tih1 maps to distal mouse Chr 1 and human Chr 1q31, chromosomal regions that have not shown evidence for imprinting and, in contrast to Ipl, Tih1 is expressed equally from both parental alleles. Ipl, Tih1, and TDAG51 have overlapping but distinct patterns of expression. Tih1 and TDAG51 are expressed in multiple fetal and adult tissues. In contrast, during early mouse development Ipl mRNA and protein are highly specific for two tissues involved in maternal/fetal exchange: visceral endoderm of the yolk sac and labyrinthine trophoblast of the placenta. These findings highlight the dominance of chromosomal context over gene structure in some examples of parental imprinting and extend previous evidence for placenta-specific expression of imprinted genes. The data also define a new subfamily of PH domain genes.

Published
1999

43. P58 molecules as putative receptors for major histocompatibility complex (MHC) class I molecules in human natural killer (NK) cells. Anti-p58 antibodies reconstitute lysis of MHC class I-protected cells in NK clones displaying different specificities.

Author

Moretta, A, primary, Vitale, M, additional, Bottino, C, additional, Orengo, A M, additional, Morelli, L, additional, Augugliaro, R, additional, Barbaresi, M, additional, Ciccone, E, additional, and Moretta, L, additional

Published
1993
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50. CD69-mediated pathway of lymphocyte activation: anti-CD69 monoclonal antibodies trigger the cytolytic activity of different lymphoid effector cells with the exception of cytolytic T lymphocytes expressing T cell receptor alpha/beta.

Author

Moretta, A, primary, Poggi, A, additional, Pende, D, additional, Tripodi, G, additional, Orengo, A M, additional, Pella, N, additional, Augugliaro, R, additional, Bottino, C, additional, Ciccone, E, additional, and Moretta, L, additional

Published
1991
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