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11 results on '"Chy, Hun S"'

1. Transient naive reprogramming corrects hiPS cells functionally and epigenetically

Author

Buckberry, Sam, Liu, Xiaodong, Poppe, Daniel, Tan, Jia Ping, Sun, Guizhi, Chen, Joseph, Nguyen, Trung Viet, de Mendoza, Alex, Pflueger, Jahnvi, Frazer, Thomas, Vargas-Landín, Dulce B., Paynter, Jacob M., Smits, Nathan, Liu, Ning, Ouyang, John F., Rossello, Fernando J., Chy, Hun S., Rackham, Owen J. L., Laslett, Andrew L., Breen, James, Faulkner, Geoffrey J., Nefzger, Christian M., Polo, Jose M., and Lister, Ryan

Published
2023
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2. New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells

Author

O'Brien, Carmel M, Chy, Hun S, Zhou, Qi, Blumenfeld, Shiri, Lambshead, Jack W, Liu, Xiaodong, Kie, Joshua, Capaldo, Bianca D, Chung, Tung‐Liang, Adams, Timothy E, Phan, Tram, Bentley, John D, McKinstry, William J, Oliva, Karen, McMurrick, Paul J, Wang, Yu‐Chieh, Rossello, Fernando J, Lindeman, Geoffrey J, Chen, Di, Jarde, Thierry, Clark, Amander T, Abud, Helen E, Visvader, Jane E, Nefzger, Christian M, Polo, Jose M, Loring, Jeanne F, and Laslett, Andrew L

Subjects
Medical Biotechnology, Biochemistry and Cell Biology, Biomedical and Clinical Sciences, Biological Sciences, Stem Cell Research - Induced Pluripotent Stem Cell - Human, Cancer, Stem Cell Research - Induced Pluripotent Stem Cell, Breast Cancer, Regenerative Medicine, Stem Cell Research - Embryonic - Human, Stem Cell Research, Biotechnology, 1.1 Normal biological development and functioning, Underpinning research, Development of treatments and therapeutic interventions, 5.2 Cellular and gene therapies, Generic health relevance, Animals, Antibodies, Monoclonal, Antigens, Surface, Cell Culture Techniques, Cell Differentiation, Cell Self Renewal, Down-Regulation, Embryoid Bodies, Flow Cytometry, Hematopoietic Stem Cells, Humans, Membrane Proteins, Mice, Octamer Transcription Factor-3, Pluripotent Stem Cells, Pluripotency, Human embryonic stem cells, Human iPS cells, Naive, Breast, Colorectal, Cell surface markers, Monoclonal antibodies, Technology, Medical and Health Sciences, Immunology, Biological sciences, Biomedical and clinical sciences
Abstract

The study and application of human pluripotent stem cells (hPSCs) will be enhanced by the availability of well-characterized monoclonal antibodies (mAbs) detecting cell-surface epitopes. Here, we report generation of seven new mAbs that detect cell surface proteins present on live and fixed human ES cells (hESCs) and human iPS cells (hiPSCs), confirming our previous prediction that these proteins were present on the cell surface of hPSCs. The mAbs all show a high correlation with POU5F1 (OCT4) expression and other hPSC surface markers (TRA-160 and SSEA-4) in hPSC cultures and detect rare OCT4 positive cells in differentiated cell cultures. These mAbs are immunoreactive to cell surface protein epitopes on both primed and naive state hPSCs, providing useful research tools to investigate the cellular mechanisms underlying human pluripotency and states of cellular reprogramming. In addition, we report that subsets of the seven new mAbs are also immunoreactive to human bone marrow-derived mesenchymal stem cells (MSCs), normal human breast subsets and both normal and tumorigenic colorectal cell populations. The mAbs reported here should accelerate the investigation of the nature of pluripotency, and enable development of robust cell separation and tracing technologies to enrich or deplete for hPSCs and other human stem and somatic cell types. Stem Cells 2017;35:626-640.

Published
2017

3. Reprogramming roadmap reveals route to human induced trophoblast stem cells

Author

Liu, Xiaodong, Ouyang, John F., Rossello, Fernando J., Tan, Jia Ping, Davidson, Kathryn C., Valdes, Daniela S., Schröder, Jan, Sun, Yu B. Y., Chen, Joseph, Knaupp, Anja S., Sun, Guizhi, Chy, Hun S., Huang, Ziyi, Pflueger, Jahnvi, Firas, Jaber, Tano, Vincent, Buckberry, Sam, Paynter, Jacob M., Larcombe, Michael R., Poppe, Daniel, Choo, Xin Yi, O’Brien, Carmel M., Pastor, William A., Chen, Di, Leichter, Anna L., Naeem, Haroon, Tripathi, Pratibha, Das, Partha P., Grubman, Alexandra, Powell, David R., Laslett, Andrew L., David, Laurent, Nilsson, Susan K., Clark, Amander T., Lister, Ryan, Nefzger, Christian M., Martelotto, Luciano G., Rackham, Owen J. L., and Polo, Jose M.

Published
2020
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4. Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media

Author

Daniszewski, Maciej, Nguyen, Quan, Chy, Hun S., Singh, Vikrant, Crombie, Duncan E., Kulkarni, Tejal, Liang, Helena H., Sivakumaran, Priyadharshini, Lidgerwood, Grace E., Hernández, Damián, Conquest, Alison, Rooney, Louise A., Chevalier, Sophie, Andersen, Stacey B., Senabouth, Anne, Vickers, James C., Mackey, David A., Craig, Jamie E., Laslett, Andrew L., Hewitt, Alex W., Powell, Joseph E., and Pébay, Alice

Published
2018
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5. A mouse model of spinal and bulbar muscular atrophy

Author

McManamny, Patrick, Chy, Hun S., Finkelstein, David I., Craythorn, Rebecca G., Crack, Peter J., Kola, Ismail, Cheema, Surindar S., Horne, Malcolm K., Wreford, Nigel G., OʼBryan, Moira K., de Kretser, David M., and Morrison, John R.

Published
2002

6. Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming

Author

Liu, Xiaodong, primary, Nefzger, Christian M, additional, Rossello, Fernando J, additional, Chen, Joseph, additional, Knaupp, Anja S, additional, Firas, Jaber, additional, Ford, Ethan, additional, Pflueger, Jahnvi, additional, Paynter, Jacob M, additional, Chy, Hun S, additional, O'Brien, Carmel M, additional, Huang, Cheng, additional, Mishra, Ketan, additional, Hodgson-Garms, Margeaux, additional, Jansz, Natasha, additional, Williams, Sarah M, additional, Blewitt, Marnie E, additional, Nilsson, Susan K, additional, Schittenhelm, Ralf B, additional, Laslett, Andrew L, additional, Lister, Ryan, additional, and Polo, Jose M, additional

Published
2017
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7. Glycosyltransferase ST6GAL1 contributes to the regulation of pluripotency in human pluripotent stem cells

Author

Wang, Yu-Chieh, primary, Stein, Jason W., additional, Lynch, Candace L., additional, Tran, Ha T., additional, Lee, Chia-Yao, additional, Coleman, Ronald, additional, Hatch, Adam, additional, Antontsev, Victor G., additional, Chy, Hun S., additional, O’Brien, Carmel M., additional, Murthy, Shashi K., additional, Laslett, Andrew L., additional, Peterson, Suzanne E., additional, and Loring, Jeanne F., additional

Published
2015
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8. Deep-transcriptome and ribonome sequencing redefines the molecular networks of pluripotency and the extracellular space in human embryonic stem cells

Author

Kolle, Gabriel, Shepherd, Jill L, Gardiner, Brooke, Kassahn, Karin, Cloonan, Nicole, Wood, David L, Nourbakhsh, Ehsan, Taylor, Darren F, Wani, Shivangi, Chy, Hun S, Zhou, Qi, McKernan, Kevin, Kuersten, Scott, Laslett, Andrew L, Grimmond, Sean M, Kolle, Gabriel, Shepherd, Jill L, Gardiner, Brooke, Kassahn, Karin, Cloonan, Nicole, Wood, David L, Nourbakhsh, Ehsan, Taylor, Darren F, Wani, Shivangi, Chy, Hun S, Zhou, Qi, McKernan, Kevin, Kuersten, Scott, Laslett, Andrew L, and Grimmond, Sean M

Abstract

Recent RNA-sequencing studies have shown remarkable complexity in the mammalian transcriptome. The ultimate impact of this complexity on the predicted proteomic output is less well defined. We have undertaken strand-specific RNA sequencing of multiple cellular RNA fractions (>20 Gb) to uncover the transcriptional complexity of human embryonic stem cells (hESCs). We have shown that human embryonic stem (ES) cells display a high degree of transcriptional diversity, with more than half of active genes generating RNAs that differ from conventional gene models. We found evidence that more than 1000 genes express long 5′ and/or extended 3′UTRs, which was confirmed by “virtual Northern” analysis. Exhaustive sequencing of the membrane-polysome and cytosolic/untranslated fractions of hESCs was used to identify RNAs encoding peptides destined for secretion and the extracellular space and to demonstrate preferential selection of transcription complexity for translation in vitro. The impact of this newly defined complexity on known gene-centric network models such as the Plurinet and the cell surface signaling machinery in human ES cells revealed a significant expansion of known transcript isoforms at play, many predicting possible alternative functions based on sequence alterations within key functional domains.

Published
2011

9. Deep-transcriptome and ribonome sequencing redefines the molecular networks of pluripotency and the extracellular space in human embryonic stem cells

Author

Kolle, Gabriel, primary, Shepherd, Jill L., additional, Gardiner, Brooke, additional, Kassahn, Karin S., additional, Cloonan, Nicole, additional, Wood, David L.A., additional, Nourbakhsh, Ehsan, additional, Taylor, Darrin F., additional, Wani, Shivangi, additional, Chy, Hun S., additional, Zhou, Qi, additional, McKernan, Kevin, additional, Kuersten, Scott, additional, Laslett, Andrew L., additional, and Grimmond, Sean M., additional

Published
2011
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11. Enrichment of OCT4 positive pluripotent hES cells using cell surface markers.

Author

Laslett, Andrew L., Chy, Hun S., Zhou, George Q., and Mondeh, Rhoda

Subjects
*IMMUNOGLOBULINS, *EMBRYONIC stem cells, *CELL receptors, *PROTEOGLYCANS, *CYTOLOGICAL techniques, *FLOW cytometry, *CELL culture
Abstract

The article provides details on the use of OCT4 antibodies to detect two validated human embryonic stem cells (hESC) markers including TG30 on human CD9 detection and TG343 on a proteoglycan on the surface of hESCs detection. A step-by-step method is presented which outlines the two cell surface markers combination to develop a flow cytometry assay for enrichment of live OCT4 positive cells. Results of the procedure indicates the rapid method to enrich for OCT4-positive cells.

Published
2009

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