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21 results on '"Churchill, Michael J."'

1. Disulfide bonding arrangements in active forms of the somatomedin B domain of human vitronectin

Author

Kamikubo, Yuichi, Guzman, Roberto De, Kroon, Gerard, Curriden, Scott, Scheraga, Harold A, Loskutoff, David J, Dyson, H. Jane, Neels, Jaap G., Churchill, Michael J, Dawson, Philip;, Oldziej, Stanislaw, and Jagielska, Anna;

Subjects
Interphotoreceptor retinoid-binding protein -- Research, Chemical bonds -- Research, Chemical bonds -- Chemical properties, Insulin-like growth factors -- Research, Insulin-like growth factors -- Chemical properties, Biological sciences, Chemistry
Abstract

The N-terminal cysteine-rich somatomedin B (SMB) domain of the human glycoprotien vitronectin contains the high-affinity binding sites for plasminogen activator inhibitor-1(PAI-1) and the urokinase receptor (uPAR). The studies demonstrate that the three dimensional structure of the SMB domain is extremely compact and that the disulfide bonds are packed in the center of the domain forming a covalently bonded core.

Published
2004

2. Substrate profiling of deubiquitin hydrolases with a positional scanning library and mass spectrometry

Author

Mason, Daniel E., Ek, Jared, Peters, Eric C., Harris, Jennifer L., Neels, Jaap G., Churchill, Michael J., Dawson, Philip, Oldziej, Stanislaw, and Jagielska, Anna;

Subjects
Protein metabolism -- Observations, Cellular proteins -- Research, Cellular proteins -- Chemical properties, Ubiquitin -- Research, Ubiquitin -- Chemical properties, Biological sciences, Chemistry
Abstract

Deconjugation of ubiquitin from cellular proteins is catalyzed by the deubiquitin hydrolase (DUB) family of enzymes and is an important component of the ubiquitin regulatory system affecting cellular function beyond maintenance of monomeric pools of ubiquitin. UCH-L3 efficiently cleaves small C-terminal amides of ubiquitin.

Published
2004

5. Modulation of reactivity in native chemical ligation through the use of thiol additives

Author

Dawson, Philip E., Churchill, Michael J., Ghadiri, M. Reza, and Kent, Stephen B.H.

Subjects
Polypeptides, Thiols -- Analysis, Chemistry
Abstract

Thiol additives act as fast catalysts to the formation of polypeptide chains in native chemical ligation. The transthioesterification process has been successfully used to synthesise several peptide-alpha-thioesters such as phospholipase A2. Weakly activated thioesters are manufactured in a chemical reaction using peptide-alpha-thiocarboxylate and an alkyl halide like benzyl bromide. Peptide-alpha-thiocarboxylate activity is regulated using thiol reducing agent.

Published
1997

7. Small molecule binding to an artificially created cavity at the active site of cytochrome c peroxidase

Author

Fitzgerald, Melissa M., Churchill, Michael J., McRee, Duncan E., and Goodin, David B.

Subjects
Cytochromes -- Research, Binding sites (Biochemistry) -- Analysis, Biological sciences, Chemistry
Abstract

The structural determinants necessary for the binding of small molecules to an artificially created cavity near a heme center at the active site of cytochrome c peroxidase was defined. Heme enzymes, which oxidize a chosen substrate, generate oxidized species when the potential is greater than one volt.

Published
1994

9. Abstract 1603: Patient derived tumor organoids identify actionable targets in heavily pretreated metastatic breast cancer patients

Author

Churchill, Michael J., primary, Schaub, Franz X., additional, Swan, Hallie A., additional, Rosati, Rachele, additional, Yan, Fengting, additional, Shaw, Reid C., additional, Gurley, Kay E., additional, Diaz, Robert L., additional, Pereira, Shalini C., additional, Grandori, Carla, additional, Kemp, Christopher J., additional, and Gadi, Vijayakrishna K., additional

Published
2018
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10. Abstract 1619: Personalized medicine: A CLIA-certified high-throughput drug screening platform for ovarian cancer

Author

Swan, Hallie A., primary, Rosati, Rachele, additional, Bridgwater, Caroline, additional, Churchill, Michael J., additional, Watt, Roland M., additional, Shaw, Reid C., additional, Murphy, Stephanie A., additional, Diaz, Robert L., additional, Pereira, Shalini C., additional, Schaub, Franz X., additional, and Grandori, Carla, additional

Published
2018
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11. Abstract 1605: Personalization of cancer treatments with a CLIA-certified high-complexity and high-throughput drug sensitivity test

Author

Swan, Hallie A., primary, Churchill, Michael J., additional, Rosati, Rachele, additional, Schaub, Franz X., additional, Shaw, Reid C., additional, Watt, Roland M., additional, Mendez, Eduardo, additional, Kemp, Christopher J., additional, Gadi, Vijayakrishna K., additional, Murphy, Stephanie A., additional, Bernard, Brady, additional, Diaz, Robert L., additional, Pereira, Shalini C., additional, and Grandori, Carla, additional

Published
2018
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12. Bone Marrow Myeloid Cells Regulate Myeloid-Biased Hematopoietic Stem Cells via a Histamine-Dependent Feedback Loop

Author

Chen, Xiaowei, primary, Deng, Huan, additional, Churchill, Michael J., additional, Luchsinger, Larry L., additional, Du, Xing, additional, Chu, Timothy H., additional, Friedman, Richard A., additional, Middelhoff, Moritz, additional, Ding, Hongxu, additional, Tailor, Yagnesh H., additional, Wang, Alexander L.E., additional, Liu, Haibo, additional, Niu, Zhengchuan, additional, Wang, Hongshan, additional, Jiang, Zhengyu, additional, Renders, Simon, additional, Ho, Siu-Hong, additional, Shah, Spandan V., additional, Tishchenko, Pavel, additional, Chang, Wenju, additional, Swayne, Theresa C., additional, Munteanu, Laura, additional, Califano, Andrea, additional, Takahashi, Ryota, additional, Nagar, Karan K., additional, Renz, Bernhard W., additional, Worthley, Daniel L., additional, Westphalen, C. Benedikt, additional, Hayakawa, Yoku, additional, Asfaha, Samuel, additional, Borot, Florence, additional, Lin, Chyuan-Sheng, additional, Snoeck, Hans-Willem, additional, Mukherjee, Siddhartha, additional, and Wang, Timothy C., additional

Published
2017
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13. Histidine decarboxylase (HDC)-expressing granulocytic myeloid cells induce and recruit Foxp3+ regulatory T cells in murine colon cancer

Author

Chen, Xiaowei, primary, Takemoto, Yoshihiro, additional, Deng, Huan, additional, Middelhoff, Moritz, additional, Friedman, Richard A., additional, Chu, Timothy H., additional, Churchill, Michael J., additional, Ma, Yan, additional, Nagar, Karan K., additional, Tailor, Yagnesh H., additional, Mukherjee, Siddhartha, additional, and Wang, Timothy C., additional

Published
2017
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14. Su1865 Bacterial Infection Contributes to Inflammation-Associated Cancer Progression via Increased Trafficking of HDC-Expressing Neutrophils

Author

Chen, Xiaowei, primary, Nagar, Karan K., additional, Chu, Timothy H., additional, Jiang, Zhengyu, additional, Chang, Wenju, additional, Friedman, Richard A., additional, Ge, Zhongming, additional, Sakitani, Kosuke, additional, Hayakawa, Yoku, additional, Tailor, Yagnesh, additional, Wang, Edwin, additional, Worthley, Daniel L., additional, Churchill, Michael J., additional, Mukherjee, Siddhartha, additional, Erdman, Susan, additional, Fox, James G., additional, and Wang, Timothy C., additional

Published
2016
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15. IL-17 producing mast cells promote the expansion of myeloid-derived suppressor cells in a mouse allergy model of colorectal cancer

Author

Chen, Xiaowei, primary, Churchill, Michael J., additional, Nagar, Karan K., additional, Tailor, Yagnesh H., additional, Chu, Timothy, additional, Rush, Brittany S., additional, Jiang, Zhengyu, additional, Wang, Edwin B.C., additional, Renz, Bernhard W., additional, Wang, Hongshan, additional, Fung, Ming Chiu, additional, Worthley, Daniel L., additional, Mukherjee, Siddhartha, additional, and Wang, Timothy C., additional

Published
2015
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17. Inhibition, Escape, and Attenuated Growth of Severe Acute Respiratory Syndrome Coronavirus Treated with Antisense Morpholino Oligomers

Author

Neuman, Benjamin W., primary, Stein, David A., additional, Kroeker, Andrew D., additional, Churchill, Michael J., additional, Kim, Alice M., additional, Kuhn, Peter, additional, Dawson, Philip, additional, Moulton, Hong M., additional, Bestwick, Richard K., additional, Iversen, Patrick L., additional, and Buchmeier, Michael J., additional

Published
2005
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19. Histidine decarboxylase (HDC)-expressing granulocytic myeloid cells induce and recruit Foxp3+regulatory T cells in murine colon cancer

Author

Chen, Xiaowei, Takemoto, Yoshihiro, Deng, Huan, Middelhoff, Moritz, Friedman, Richard A., Chu, Timothy H., Churchill, Michael J., Ma, Yan, Nagar, Karan K., Tailor, Yagnesh H., Mukherjee, Siddhartha, and Wang, Timothy C.

Abstract

ABSTRACTThe colorectal tumor microenvironment contains a diverse population of myeloid cells that are recruited and converted to immunosuppressive cells, thus facilitating tumor escape from immunoediting. We have identified a genetically and functionally distinct subset of dynamic bone marrow myeloid cells that are characterized by histidine decarboxylase (HDC) expression. Lineage tracing in Hdc-CreERT2;R26-LSL-tdTomato mice revealed that in homeostasis, there is a strong bias by HDC+myeloid cells toward the CD11b+Ly6Ghigranulocytic lineage, which was accelerated during azoxymethane/dextran sodium sulfate (AOM/DSS)-induced colonic carcinogenesis. More importantly, HDC+myeloid cells strongly promoted colonic tumorigenesis, and colon tumor progression was profoundly suppressed by diphtheria toxin A (DTA)-mediated depletion of HDC+granulocytic myeloid cells. In addition, tumor infiltration by Foxp3+regulatory T cells (Tregs) was markedly impaired following HDC+myeloid cell depletion. We identified an HDC+myeloid-derived Cxcl13/Cxcr5 axis that mediated Foxp3 expression and Treg proliferation. Ablation of HDC+myeloid cells or disruption of the Cxcl13/Cxcr5 axis by gene knockdown impaired the production and recruitment of Tregs. Cxcl13 induction of Foxp3 expression in Tregs during tumorigenesis was associated with Stat3 phosphorylation. Overall, HDC+granulocytic myeloid cells affect CD8+T cells directly and indirectly through the modulation of Tregs and thus appear to play key roles in suppressing tumoricidal immunity.

Published
2017
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20. Shutting Bad Schools, Helping Students.

Author

Churchill, Michael J. Petrilli And Aaron

Subjects
*SCHOOL administration, *INTERNATIONAL relations, *EDUCATION policy, *CHILD development
Published
2015

21. Histidine decarboxylase (HDC)-expressing granulocytic myeloid cells induce and recruit Foxp3 + regulatory T cells in murine colon cancer.

Author

Chen X, Takemoto Y, Deng H, Middelhoff M, Friedman RA, Chu TH, Churchill MJ, Ma Y, Nagar KK, Tailor YH, Mukherjee S, and Wang TC

Abstract

The colorectal tumor microenvironment contains a diverse population of myeloid cells that are recruited and converted to immunosuppressive cells, thus facilitating tumor escape from immunoediting. We have identified a genetically and functionally distinct subset of dynamic bone marrow myeloid cells that are characterized by histidine decarboxylase (HDC) expression. Lineage tracing in Hdc-CreERT2;R26-LSL-tdTomato mice revealed that in homeostasis, there is a strong bias by HDC + myeloid cells toward the CD11b + Ly6G hi granulocytic lineage, which was accelerated during azoxymethane/dextran sodium sulfate (AOM/DSS)-induced colonic carcinogenesis. More importantly, HDC + myeloid cells strongly promoted colonic tumorigenesis, and colon tumor progression was profoundly suppressed by diphtheria toxin A (DTA)-mediated depletion of HDC + granulocytic myeloid cells. In addition, tumor infiltration by Foxp3 + regulatory T cells (Tregs) was markedly impaired following HDC + myeloid cell depletion. We identified an HDC + myeloid-derived Cxcl13/Cxcr5 axis that mediated Foxp3 expression and Treg proliferation. Ablation of HDC + myeloid cells or disruption of the Cxcl13/Cxcr5 axis by gene knockdown impaired the production and recruitment of Tregs. Cxcl13 induction of Foxp3 expression in Tregs during tumorigenesis was associated with Stat3 phosphorylation. Overall, HDC + granulocytic myeloid cells affect CD8 + T cells directly and indirectly through the modulation of Tregs and thus appear to play key roles in suppressing tumoricidal immunity.

Published
2017
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