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A hydroxypropyl-β-cyclodextrin (HP-β-CD) imprinted coating based on polyhedral oligomeric silsesquioxane (POSS) for open tubular electrochromatography was prepared. The mixture of methacryl-POSS (MA0735), HP-β-CD (template), methacrylic acid (MAA, monomer)

Revealing the turbulent drag reduction mechanism of water flow on microstructured surfaces is beneficial to controlling and using this technology to reduce turbulence losses and save energy during water transportation. Two microstructured samples, including a superhydrophobic and a riblet surface, were fabricated near which the water flow velocity, and the Reynolds shear stress and vortex distribution were investigated using a particle image velocimetry. The dimensionless velocity was introduced to simplify the Ω vortex method. The definition of vortex density in water flow was proposed to quantify the distribution of different strength vortices. Results showed that the velocity of the superhydrophobic surface (SHS) was higher compared with the riblet surface (RS), while the Reynolds shear stress was small. The vortices on microstructured surfaces were weakened within 0.2 times that of water depth when identified by the improved ΩM method. Meanwhile, the vortex density of weak vortices on microstructured surfaces increased, while the vortex density of strong vortices decreased, proving that the reduction mechanism of turbulence resistance on microstructured surfaces was to suppress the development of vortices. When the Reynolds number ranged from 85,900 to 137,440, the drag reduction impact of the superhydrophobic surface was the best, and the drag reduction rate was 9.48%. The reduction mechanism of turbulence resistance on microstructured surfaces was revealed from a novel perspective of vortex distributions and densities. Research on the structure of water flow near the microstructured surface can promote the drag reduction application in the water field.

In the field of the Internet of Things, sensors with large-scale perception ability are urgently needed for the scenarios, such as structure health monitoring, vehicle tracking, and so on. Quasi-distributed acoustic sensing (QDAS), which uses a sensing fiber with an ultraweak reflector array to perceive thousands of points at the same time, shows great advantages in these applications. The sensing fiber integrates sensing and transmission functionalities together and is easy to be deployed on large-scale targets. With the development of QDAS, the performance limitations caused by the finite frequency-domain resource are gradually emerging. Multiplexing the frequency-domain resource can be the key to further performance breakthroughs. For this purpose, the multiple-input–multiple-output (MIMO) technology, which has been widely used in communication/radar systems, is introduced into QDAS in this article. The principles of QDAS with the MIMO technology are elaborated, and the feasibility is verified through simulations and experiments, in which the response bandwidth is tripled compared to the traditional single-pulse QDAS. To the best of our knowledge, this is the first time that the MIMO coding technology, i.e., orthogonal codes with the same frequency (OCSF), is used in QDAS. This work paves a new way for breaking QDAS performance limitations that are bounded by the finite frequency resource.

Regional water demand is an important basic data for regional engineering planning, design and management. Making full use of multi-source data and prior knowledge to quickly and economically obtain high-precision regional water demand is of great significance to the optimal allocation of regional water resources. In order to accurately predict the regional water demand, this study took Yulin City as a research area to predict the water demand of the city from 2017 to 2019. Aiming at the oscillating characteristics of the regional water demand sequence and the over-fitting problem of traditional prediction models, this study proposed the non-dominated sorting genetic algorithm II-fractional order reverse accumulative grey model (NSGAII-FORAGM). The regional water demand oscillation sequence was transformed into a monotonically decreasing non-negative sequence. Based on the transformation sequence, an optimization model was constructed according to the two objective functions of ‘maximum (or minimum) order’ and ‘best fit to historical data’, and the NSGAII method was adopted to solve the model. The three model structures of ‘fractional order’, ‘reverse accumulation’ and ‘obtaining order through multi-objective optimization model ‘ were tested based on the water use sequence of the three sectors (industry, tertiary industry and domestic) in Yulin City, and the performance of the method is compared with NSGAII-IORAGM, NSGAII-FOFAGM and SOGA-FORAGM. The results showed that the average relative error of the model established in this study for the simulation of industry, tertiary industry (the tertiary industry is a technical name for the service sector of the economy, which encompasses a wide range of businesses), and domestic was 15.54%, 11.20%, 9.98% respectively. The average relative error of the model established in this study for the prediction of industry, tertiary industry and domestic was 9.46%, 7.9%, and 1.8%, respectively. For the simulation of water demand sequences in three sections, the simulation average relative errors of the other three models were not absolutely dominant except for the SOGA-FORAGM model. The average relative predicted error by the model in this study was the smallest (the relative errors of the three sequence predictions for industry, tertiary industry and domestic were lower than the relative errors of the optimal results of the comparison model, which were 0.97%, 0.72% and 4.5%, respectively), indicating that the model had certain applicability for the water demand prediction of various sectors (industry, tertiary industry and domestic) in the region compared with other models, and can improve the accuracy of the prediction results.

Open-channel bifurcations are the most common water diversion structures in irrigation districts. In irrigation water conveyance, water transport efficiency and sedimentation are primary concerns. This study accordingly analyzes the influence of open-channel bifurcations on water delivery in irrigation areas. Herein, the three-dimensional flow at an open-channel bifurcation was studied via numerical simulations using FLOW-3D software and including 15 sets of working conditions. The hydraulic characteristics of the recirculation zone and flow structures in the vicinity of the open-channel bifurcation were analyzed. Equations for the flow diversion width of the surface and bottom layers in the trapezoidal channel were then obtained. The flow diversion widths along the water depth were found to differ between trapezoidal and rectangular channels. The results also show that open-channel bifurcations considerably influence the flow velocity in the main channel. The flow velocity in the recirculation zone of open-channel bifurcations was small, but the pulsation velocity and the turbulent kinetic energy were large. The energy dissipated in this area was relatively large, which was not conducive to channel water delivery. This study provides a reference for channel optimization and operation management in irrigation districts.

A fluorescent probe for the colorimetric detection of cysteine (Cys) was synthesized by combining resorufin with 7-nitrobenzofurazan. The resultant probe was characterized by FT-IR spectroscopy, fluorescence detection, mass spectrometry and NMR spectroscopy. After reacting with Cys, resorufin is released and the color changes from light yellow to red on paper-based analytical devices. The results were recorded using a common cell phone and subsequently analyzed using Photoshop software. The color intensity of the RGB channel increased linearly in the 0.04 to 70.04 μM Cys concentration range when the probe concentration was 2.6 mM (R = 0.9965), and the limit of detection was 16 nM. The effects of detection conditions have been investigated and are discussed. The interference deviations of 13 substances to Cys were in the range of -2% to +2%. The Cys concentration was determined as 270.8 ± 19.3 μM in human plasma, and the recovery was from 99.7% to 100.4%. The work demonstrates the potential of the method to detect Cys in real samples with low cost and high sensitivity.

Ovarian surface epithelium (OSE) undergoes recurring ovulatory rupture and OSE stem cells rapidly generate new cells for the repair. How the stem cell senses the rupture and promptly turns on proliferation is unclear. Our previous study has identified that Protein C Receptor (Procr) marks OSE progenitors. In this study, we observed decreased adherent junction and selective activation of YAP signaling in Procr progenitors at OSE rupture site. OSE repair is impeded upon deletion of Yap in these progenitors. Interestingly, Procr+ progenitors show lower expression of Vgll4, an antagonist of YAP signaling. Overexpression of Vgll4 in Procr+ cells hampers OSE repair and progenitor proliferation, indicating that selective low Vgll4 expression in Procr+ progenitors is critical for OSE repair. In addition, YAP activation promotes transcription of the OSE stemness gene Procr. The combination of increased cell division and Procr expression leads to expansion of Procr+ progenitors surrounding the rupture site. These results illustrate a YAP- dependent mechanism by which the stem/progenitor cells recognize the ovulatory rupture, and rapidly multiply their numbers, highlighting a YAP- induced stem cell expansion strategy.

Ovarian surface epithelium (OSE) undergoes recurring ovulatory rupture and OSE stem cells rapidly generate new cells for the repair. How the stem cell activation is triggered by the rupture and promptly turns on proliferation is unclear. Our previous study has identified that Protein C Receptor (Procr) marks OSE progenitors. In this study, we observed decreased adherent junction and selective activation of YAP signaling in Procr progenitors at OSE rupture site. OSE repair is impeded upon deletion of Yap1 in these progenitors. Interestingly, Procr+ progenitors show lower expression of Vgll4, an antagonist of YAP signaling. Overexpression of Vgll4 in Procr+ cells hampers OSE repair and progenitor proliferation, indicating that selective low Vgll4 expression in Procr+ progenitors is critical for OSE repair. In addition, YAP activation promotes transcription of the OSE stemness gene

The protein C receptor (Procr) has been implicated as a stem cell surface marker in several tissues. It is unknown whether Procr acts as a functional signaling receptor in stem cells. Here, by conditional knockout in mammary stem cells (MaSCs), we demonstrate that Procr is essential for mammary gland development and homeostasis. Through proteomics profiling, we identify that, upon stimulation by the ligand protein C, Procr interacts with heat shock protein 90 (HSP90AA1) via its short cytoplasmic tail, recruiting Src and IGF1R to the complex at the plasma membrane. We show that Procr acts as a signaling receptor of protein C in regulation of MaSCs through HSP90, Src, and IGF1R in vitro. In vivo, IGF1R deletion in MaSCs displays similar phenotypes to Procr deletion. These findings illustrate the essential role of Procr signaling in MaSC maintenance, shedding light onto the molecular regulation by Procr in tissue stem cells.

Tumor metastasis is the leading cause of cancer-associated mortality. Unfortunately, the underlying mechanism of metastasis is poorly understood. Expression of legumain (LGMN), an endo-lysosomal cysteine protease, positively correlates with breast cancer metastatic progression and poor prognosis. Here, we report that LGMN is secreted in the zymogen form by motile breast cancer cells. Through binding to cell surface integrin αvβ3 via an RGD motif, the autocrine pro-LGMN activates FAK-Src-RhoA signaling in cancer cells and promotes cancer cell migration and invasion independent of LGMN protease activity. Either silencing LGMN expression or mutationally abolishing pro-LGMN‒αvβ3 interaction significantly inhibits cancer cell migration and invasion in vitro and breast cancer metastasis in vivo. Finally, we developed a monoclonal antibody against LGMN RGD motif, which blocks pro-LGMN‒αvβ3 binding, and effectively suppresses cancer cell migration and invasion in vitro and breast cancer metastasis in vivo. Thus, disruption of pro-LGMN‒integrin αvβ3 interaction may be a potentially promising strategy for treating breast cancer metastasis.

Alzheimer's disease (AD) is the most common cause of dementia in elderly individuals. Currently, acetylcholinesterase inhibitors (AChEI) are the most effective clinical treatment for AD. AChEIs in natural products may have therapeutic potential and should be screened for use in AD treatment. The authors describe a simple and reliable method for AChEI screening by capillary electrophoresis (CE). A hexadimethrine bromide (HDB) solution was pushed into a capillary (0.015 MPa×10 s) and incubated for 5 min. The capillary was flushed with deionized water for 5 min to remove free HDB, followed by plugging with an acetylcholinesterase (AChE) solution. After a 5 min incubation, the AChE was immobilized on the positively charged coating by ion binding, and the micro-reactor was created. The substrate solution, acetylthiocholine iodide (AThC), was injected into the capillary and incubated in the micro-reactor for 1 min. The unreacted substrate and the enzymolysis product were separated by CE. Gastrodin, an important component of

Breast cancer is a heterogeneous disease. In particular, triple-negative breast cancer (TNBC) comprises various molecular subgroups with unclear identities and currently has few targeted treatment options. Our previous study identified protein C receptor (Procr) as a surface marker on mammary stem cells (MaSCs) located in the basal layer of the normal mammary gland. Given the possible connection of TNBC with basal layer stem cells, we conducted comparative analyses of Procr in breast cancers of mouse and human origin. In mouse mammary tumors, we showed that Procr(+) cells are enriched for cancer stem cells (CSCs) in Wnt1 basal-like tumors, but not in Brca1 basal-like tumors or PyVT luminal tumors. In human cancers, PROCR was robustly expressed in half of TNBC cases. Experiments with patient-derived xenografts (PDXs) revealed that PROCR marks CSCs in this discrete subgroup (referred to as PROCR(+) TNBC). Interfering with the function of PROCR using an inhibitory nanobody reduced the CSC numbers, arrested tumor growth and prevented rapid tumor recurrence. Our data suggest a key role of MaSC in breast tumorigenesis. Moreover, our work indicates that PROCR can be used as a biomarker to stratify TNBC into clinically relevant subgroups and may provide a novel targeted treatment strategy for this clinically important tumor subtype.

We report the colorimetric detection of dopamine (DA) on microfluidic paper-based analytical devices (μPADs) using an oxidation-reduction method. Here, dopamine reacts with ferric chloride forming reduced Fe2+ that subsequently reacts with phenanthroline to form the red tris(1,10-phenanthroline)iron(II) complex. The devices were fabricated by wax printing and changes in color intensity were recorded using a common cell phone. Subsequent analysis using Photoshop software, yielded a limit of detection (LOD) for DA of 0.37 μmol/L with a linear range of 0.527–4.75 μmol/L and relative standard deviation of 0.11% (inter-day) and 0.15% (intra-day) for n = 15 paper chips. The effects of detection conditions have been investigated and are discussed. Cow serum samples and human blood serum and plasma samples were detected. The work, herein, demonstrates the potential of this method as a low cost and rapid colorimetric technique to detect DA in real samples.

This publisher’s note contains corrections to Opt. Lett. 46, 685 (2021)OPLEDP0146-959210.1364/OL.415087.

Quasi-distributed acoustic sensing (Q-DAS) based on ultra-weak fiber Bragg grating (UWFBG) is currently attracting great attention, due to its high sensitivity and excellent multiplexing capability. Phase-sensitive optical time-domain reflectometry (Φ-OTDR) based on phase demodulation is one of the most promising interrogation schemes for Q-DAS. In this article, a novel interleaved identical chirped pulse (IICP) approach is proposed on the basis of pulse compression Φ-OTDR with coherent detection. Different from the frequency-division-multiplexing (FDM) method, the identical pulses are used for multiplexing in the IICP scheme, and the mixed reflection signals can be demodulated directly, so the inconsistent phase offsets in FDM can be avoided. As a result, this scheme can enlarge the measurement slew-rate (SR) of Q-DAS by times compared with traditional single pulse scheme. In the proof-of-principle experiment, the SR of 28.9 mɛ/s has been achieved with an 860 m sensing range, which is 5 times as that of the traditional single pulse scheme; meanwhile, the response bandwidth has been enlarged by 5 times. The 277 kHz response bandwidth has been achieved, with 5 m spatial resolution and 2.8 p ε / Hz strain sensitivity.

Irrigation pressures and filters have a significant influence on emitter clogging in drip fertigation systems. Exploring the anti-clogging performance of emitters at different irrigation pressures (80, 90, 100 kPa; 40, 50, 60 kPa) and filters (the disc filter with an aperture of 125 μm, the screen filter with an aperture of 125μm, and the screen filter with an aperture of 200 μm) can provide suitable guidance for the design and operation of drip fertigation systems. The average relative discharge (Dra), the Christiansen uniformity coefficient (CU), and the differential pressure (DP) were analyzed to study the anti-clogging performance of flat emitters (FE) and labyrinth emitters (LE). Meanwhile, the scanning electron microscope and energy dispersive spectrometer were used to observe the surface morphology and chemical composition of clogging substances in filters and emitters. Results showed that the irrigation pressure of 90 and 50 kPa did not cause a significant decrease in Dra, and the Dra of two types of emitters were all great than 95.22%. When the irrigation pressure was 80 and 40 kPa, the Dra decreased significantly (73.48–78.71%, 81.3–83.6% for FE and LE). The CU values of emitters were all greater than 90% except for the irrigation pressures of 80 and 40 kPa. The relative Dra and CU of LE were greater than those of FE, indicating that the LE had a better anti-clogging performance compared with the FE. The decrease in DP reflected an increase in the emitter clogging degree. Meanwhile, irrigation pressures and the interaction between irrigation pressures and filters imposed a significant effect on the CU at a significance level of p < 0.05, p < 0.01, respectively. The clogging process was mainly affected by physical and chemical factors, and the generated clogging substances included phosphate fertilizer, silicate, carbonate, and phosphate precipitations. In conclusion, the best operation mode of phosphate fertilizer in drip fertigation systems was to use the disc filter with an aperture of 125 μm and the LE at the irrigation pressure of 50 kPa. An appropriate irrigation pressure combined with effective filters can reduce emitter clogging while saving water and fertilizer.

Protein kinase G (PknG), a eukaryotic type serine-threonine protein kinase (STPK) inMycobacterium tuberculosis(Mtb), is secreted into the cytosol of infected macrophages to promote intracellular survival of mycobacteria and has been considered as a promising therapeutic target for tuberculosis (TB) treatment. However, the molecular details of Mtb PknG-host intracellular interactions remain obscure. Here, we demonstrate that PknG serves as both the ubiquitin-activating enzyme (E1) and the ubiquitin ligase (E3) to promote ubiquitination and degradation of tumor necrosis factor receptor-associated factor 2 (TRAF2) and TGF–β-activated kinase 1 (TAK1), and thus inhibits the NF-κB-mediated host innate immune responses. Surprisingly, PknG promotes the attachment of ubiquitin (Ub) to ubiquitin-conjugating enzyme (E2) UbcH7 via an isopeptide bond (UbcH7 K82-Ub), instead of a usual C86-Ub thiol-ester bond, and then promotes the discharge of Ub from UbcH7 by acting as an isopeptidase before attaching Ub to its substrates TRAF2 and TAK1. These results demonstrate that Mtb PknG promotes ubiquitination of the key components of the host innate immunity by acting as an unusual ubiquitinating enzyme to suppress innate immunity. Our findings provide a potential TB treatment via targeting unconventional ubiquitinating activities of PknG.SignificanceMycobacterium tuberculosis(Mtb) protein kinase G (PknG), which is critical for Mtb intracellular survival, is a promising target for tuberculosis (TB) treatment. However, the molecular mechanisms underlying PknG-host interactions remain largely unclear. Here we demonstrate that PknG serves as both the ubiquitin-activating enzyme and the ubiquitin ligase to promote the ubiquitination and degradation of tumor necrosis factor receptor-associated factor 2 (TRAF2) and TGF-β-activated kinase 1 (TAK1), thus inhibiting NF-κB signaling activation. PknG promotes the attachment of ubiquitin to ubiquitin-conjugating enzyme UbcH7 via an isopeptide bond, instead of a usual thiol-ester bond, and releases the ubiquitin from UbcH7 by acting as an isopeptidase. These findings provide important information for rational development of TB treatment via targeting unconventional ubiquitinating activity of PknG.

Insulin production is required for glucose homeostasis. Pancreatic islet β cells are the only cells that produce insulin in humans; however, generation of functional β cells in vitro from embryonic or adult tissues has been challenging. Here, we describe isolation of pancreatic islet progenitors from adult mice, which enables the efficient generation and long-term expansion of functional islet organoids in vitro. This protocol starts with purification of protein C receptor (Procr)-expressing islet progenitors. Coculture with endothelial cells generates islet organoids in vitro that can be expanded by passage. Functional maturation is achieved as a consequence of a prolonged culture period and cyclic glucose stimulation. Primary islet organoids form in 7-10 days. Subsequently, each passage takes 1 week, with the final maturation step requiring 3 weeks of additional culture. The resulting organoids are predominantly composed of β cells but also contain small proportions of α, δ and pancreatic polypeptide cells. The organoids sense glucose and secrete insulin. This approach thus provides a strategy for β cell generation in vitro and an organoid system to study islet regeneration and diseases.

An open-tubular capillary electrochromatography method has been developed for the determination of binding constants between β2 -adrenergic receptor (β2 -AR) and seven drugs. β2 -AR was oriented immobilized onto one part of inner surface of capillary via microwave-assisted technical synthesis. According to the linear relationship between coating length and the apparent mobility of analyte, the binding constant (Kb ) can be obtained by related theories and equations. The order of Kb values between drugs such as adrenaline hydrochloride, norepinephrine bitartrate, and propranolol hydrochloride with β2 -AR is well consistent with that reported in the literature. By the method, Kb values between four extracts of Radix Paeoniae Rubra and β2 -AR were also successfully obtained. Subsequently, computer models were applied to interpret the CEC experiments. And the results proved to be in good agreement with the method. The work, herein, demonstrates the potential of the method in drug-receptor affinity interactions evaluation and screening of lead compounds from natural sources.

A simple capillary zone electrophoresis (CZE) method was developed to achieve the rapid enantiomeric separation of a set of acidic drugs using streptomycin as chiral selector. The enantiomers of 5 chiral phenyl-containing acidic pharmaceutical compounds were separated excellently by CE using an uncoated silica capillary. Several experimental parameters such as the concentration of streptomycin, buffer concentration and pH, running voltage, and capillary temperature were all investigated systematically in order to optimize the chiral separation. All analytes were got baseline separation within 10 min. And the results showed that streptomycin can be used as a chiral selector to the enantioseparation of five acidic drugs by CZE method.

A method is described for cysteine (Cys) determination on paper-based analytical devices using aspartic acid modified gold nanoparticles (Asp-AuNPs). The Asp-AuNPs were characterized by their size, zeta potential, and UV-visible absorption spectrum. After the addition of Cys, it will interact with Asp-AuNPs selectively and leads to the aggregation of Asp-AuNPs. A color change from red to blue can be observed on the paper-based analytical devices. The results were recorded using a cell phone and subsequently analyzed using the Photoshop software. The ratiometric color intensity at red channel and blue channel (Red/Blue) increased linearly in the range 99.9-998.7 μM for Cys (R = 0.9984), and the limit of detection was 1.0 μM. The effects of assay conditions have been investigated and are discussed. The Cys concentration was determined as (0.27 ± 0.02 mM) in human plasma, and the recovery was from 99.2 to 101.1%. Graphical abstract Schematic representation of the paper-based assay system using aspartic acid modified gold nanoparticles (Asp-AuNPs). The ratiometric color intensity method was used for the cysteine (Cys) determination.

Drip irrigation using fertilizers is one of the commonly used methods to save fertilizer and minimize the impacts of fertilizer on the soil and the environment. However, the clogging issue of drip fertigation systems has been the major limitation and may lead to system damage if fertilizer solution concentrations are not appropriately selected. Therefore, the head loss, flow rates, and clogging substances of screen and disc filters were analyzed to evaluate the effect of different fertilizer solution concentrations on filter clogging under different injection rates (2%, 3%, and 4%) and fertilizer-water ratios (1:4, 1:5, and 1:6) in drip fertigation systems. The surface morphology and chemical composition of the clogging substances were analyzed by scanning electron microscopy and energy dispersive spectrometry (EDS). The results showed that the large injection rate caused uneven fertilization, and the recommend injection rates of the proportional fertilizer pump were 2% and 3%. In the same fertilizer solution concentration range, the difference in head loss of the screen filter (0.09–7.75 m) was larger than that of the disc filter (0.32–3.88 m), indicating that the anti-clogging performance of the disc filter was better. Meanwhile, the application scope of the fertilizer solution concentration of the screen filter was less than 0.117% and that of the disc filter was between 0.117% and 0.296%. There was a significant effect of the fertilizer solution concentrations on filter clogging (P

This Letter proposes a novel phase-sensitive optical time domain reflectometry ( Φ -OTDR) with continuous chirped-wave (CCW), which can make full use of both time and frequency domain resources. The principle and benefits of CCW Φ -OTDR are elaborated. With the merit of CCW Φ -OTDR, 1.042 MHz sensing bandwidth and 5 p ε / H z strain sensitivity are achieved along a 1013 m fiber with 4.4 m spatial resolution. To the best of the authors’ knowledge, this is the first time that a Φ -OTDR achieves megahertz sensing bandwidth with metric spatial resolution, and without limiting the frequency feature of the disturbance. The good performance in long-range sensing is also verified over a 49.7 km fiber. More than that, the digital domain flexibility of the proposed scheme can be used to optimize the measured acoustic signal according to its feature and the practical needs.

Boronate affinity is an important method for the enrichment and separation of cis-diol containing compounds, but most of the conventional boronate materials suffer from weak binding strength as well as low binding capacity towards glycoproteins due to the use of single boronic acids as ligands. In this work, a novel multidentate boronate magnetic adsorbent was assembled by using amined polyhedral oligomeric silsesquioxane as spacer and a diboronic acid as ligand. The specially designed adsorbent exhibited high adsorption capacity for cis-diols due to the high density of phenylbronic acid moieties. More interestingly, the dissociation constants toward glycoproteins on the material were lowered to be ∼10−6 M, being at least 3 orders lower than the single boronic acid bonded adsorbents. By comparing the binding properties of small molecules containing one and two pairs of cis-diols, the enhanced binding strength of glycoproteins on the multidentate boronate magnetic adsorbent was attributed to the synergistic binding of glycoproteins on the special interface. The new materials successfully captured glycoproteins from 1000-fold diluted egg white, suggesting that the material could be an optional alternative adsorbent for enriching trace glycoproteins from complex bio-samples.

An open-tubular capillary electrochromatography method has been developed for the determination of binding constants between β

Enantiomeric separations of the adrenergic compounds adrenaline, noradrenaline, and isoprenaline were studied. Electromigrative separations were performed in uncoated fused silica capillaries using streptomycin-modified gold nanoparticles (ST-AuNPs) as an additive to the background electrolyte. The ST-AuNPs are shown to serve as an effective chiral selector. The modified AuNPs were characterized in terms of size and zeta potential, and by IR and UV-vis spectra. The effects of ST-AuNP concentration, pH value, temperature, and separation voltage on the separations were systematically studied. Under optimized experimental conditions, racemic mixtures of the respective adrenergic drugs were baseline-separated within 7 min with a resolution of up to 7.5. The relative standard deviations of the resolution in inter-day and intra-day studies (n = 5) were generally5%. Graphical abstract Schematic of the method for enantiomeric separations. (A): At low concentrations of streptavidinylated gold nanoparticles (ST-AuNPs), the better matching enantiomer is preferably "transported" by the ST-AuNPs; (B) ST-AuNP concentration increased to an optimal value; (C): The ST-AuNP concentration is too high; even poorly matching enantiomers will be transported simultaneously.

Oriented covalent immobilized β2 -adrenergic receptor (β2 -AR) CE (OIRCE) was developed to determine the interactions between a set of natural extracts of Radix Paeoniae Rubra (NERPR) and β2 -AR, and to predict the activity of NERPR. The inner capillary surface is chemically bonded with stable β2 -AR coating via microwave-assisted technical synthesis. The modified capillaries were characterized via infrared spectroscopy and fluorescence microscopy. Furthermore, the bonding amounts of β2 -AR were first obtained via fluorescence spectroscopy method. In determining the amount of bonded β2 -AR, the regression equation A = 576 707C + 35.449 and the correlation coefficient 0.9995 were obtained. This result revealed an excellent linear relationship in the range of 2 × 10(-4) mg/mL to 1 × 10(-3) mg/mL. The normalized capacity factor (KRCE ) was obtained using OIRCE in evaluating drug-receptor interactions. Related theories and equations were used to calculate KRCE values from apparent migration times of a solute and EOF. The order of KRCE and the binding constant (Kb ) values between drugs and β2 -AR was well consistent. The results confirmed that the OIRCE and KRCE values can be effectually used to investigate drug-receptor interactions, and OIRCE has the potential to predict drug activity and to select leading compounds from natural chemicals.

The paper is concerned with the novel method on polymer molecular weight and gyration radius of DNA (R g ) detection by a quantitative DNA sieving model using DNA separations by capillary electrophoresis, which was proposed by us previously. First, the molecular weights of polymer additives such as hydroxylpropyl cellulose (HPC) and hydroxyethyl cellulose (HEC) were calculated by the intercept of a linear plot of (1/μ–1/μ0)1/2 against . The relative differences between the predicted and the actual values (M w = 80, 000 to 1, 000, 000) were all within the range of −0.61% to 6.71%. Second, the radius of gyration (R g) of DNA in the collision direction of the Y-axis can be also calculated simultaneously. Results indicated that R g depends on the DNA length, polymer concentration, polymer species, and polymer M w. R g lengthens with increased DNA length, but shortens with increased solution concentration and polymer M w. Furthermore, R g of DNA was longer in linear polyacrylamide (LPA) than in HEC solution wh...