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Yeast peptides, which are small-molecule active peptides extracted from yeast proteins, are known for their antibacterial, antioxidant, and anti-inflammatory properties. However, the effects of yeast peptide on suckling calves remain unclear. In this study, the effects of yeast peptide supplementation on the growth, diarrhea incidence, and immune function of calves during the suckling period were determined. Thirty newborn calves were randomly divided into two groups: the control group (CON) and the treatment group (AP), which received fresh pasteurized milk supplemented with yeast peptides (5 g/day). The experiment lasted for 49 days (7–56 days of age). The dry matter intake, body weight, diarrhea status, immune function, antioxidant capacity, and anti-inflammatory activity of the calves were analyzed. The AP group had higher dry matter intake, daily weight gain, and feed efficiency than the CON group (P < 0.05). Moreover, the duration and frequency of diarrhea were significantly lower in the AP group than in the CON group (P < 0.05). Furthermore, the immune, antioxidant, and anti-inflammatory capabilities of the AP group were significantly higher than those of the CON group (P < 0.05). These findings provide valuable insights for the improvement of early health management during calf rearing.

Elucidating the plant−microbiome network is of importance in understanding species coexistence in natural ecosystems. The phyllosphere, which is the aerial parts of terrestrial plants, is inhabited by diverse microbes. However, few studies have focused on the phyllosphere microbiome and plant-microbiome network in temperate grasslands. In this study, we explored the diversity, community structure, and network architecture of phyllosphere bacteria and fungi in 19 plant species native to the temperate grassland in Inner Mongolia, China. We obtained 3,313 and 758 phyllosphere bacterial and fungal operational taxonomic units, respectively, and found that the bacterial community was dominated by Proteobacteria, Actinobacteriota, and Firmicutes, whereas the fungal community was dominated by Ascomycota and Basidiomycota. Plant identity exerted significant impacts on α-diversities of both bacterial and fungal communities. The composition of bacterial and fungal communities differed among plant species. Plant identity had a greater effect on fungal than bacterial communities. Both bacterial and fungal network structures were characterized by specialized, modular, lowly connected, and no nested properties. The plant−fungal network had a high level of specification, modularity, antinestedness, and connectance compared with the plant−bacterial network. Our results suggest more intimate relationships between plants and phyllosphere fungi than between plants and phyllosphere bacteria and also that the phyllosphere fungal community in the temperate grassland ecosystem is more resistant to environmental disturbance than the phyllosphere bacterial community. These findings may contribute to our understanding of the mechanisms by which species coexist and communities stabilize in grassland ecosystems.

Abstract In recent years, human umbilical cord mesenchymal stem cell (hUC-MSC) extracellular vesicles (EVs) have been used as a cell replacement therapy and have been shown to effectively overcome some of the disadvantages of cell therapy. However, the specific mechanism of action of EVs is still unclear, and there is no appropriate system for characterizing the differences in the molecular active substances of EVs produced by cells in different physiological states. We used a data-independent acquisition (DIA) quantitative proteomics method to identify and quantify the protein composition of two generations EVs from three different donors and analysed the function and possible mechanism of action of the proteins in EVs of hUC-MSCs via bioinformatics. By comparative proteomic analysis, we characterized the different passages EVs. Furthermore, we found that adaptor-related protein complex 2 subunit alpha 1 (AP2A1) and adaptor-related protein complex 2 subunit beta 1 (AP2B1) in hUC-MSC-derived EVs may play a significant role in the treatment of Alzheimer's disease (AD) by regulating the synaptic vesicle cycle signalling pathway. Our work provides a direction for batch-to-batch quality control of hUC-MSC-derived EVs and their application in AD treatment.

The variety and content of high-quality proteins in sunflower seeds are higher than those in other cereals. However, sunflower seeds can suffer from abnormalities, such as breakage and deformity, during planting and harvesting, which hinder the development of the sunflower seed industry. Traditional methods such as manual sensory and machine sorting are highly subjective and cannot detect the internal characteristics of sunflower seeds. The development of spectral imaging technology has facilitated the application of terahertz waves in the quality inspection of sunflower seeds, owing to its advantages of non-destructive penetration and fast imaging. This paper proposes a novel terahertz image classification model, MobileViT-E, which is trained and validated on a self-constructed dataset of sunflower seeds. The results show that the overall recognition accuracy of the proposed model can reach 96.30%, which is 4.85%, 3%, 7.84% and 1.86% higher than those of the ResNet-50, EfficientNeT, MobileOne and MobileViT models, respectively. At the same time, the performance indices such as the recognition accuracy, the recall and the F1-score values are also effectively improved. Therefore, the MobileViT-E model proposed in this study can improve the classification and identification of normal, damaged and deformed sunflower seeds, and provide technical support for the non-destructive detection of sunflower seed quality.

Abstract Background With the decline of cognitive function in vascular cognitive impairment, the burden on the family and society will increase. Therefore, early identification of vascular mild cognitive impairment (VaMCI) is crucial. The focus of early identification of VaMCI is on the attention of risk factors. Therefore, this study aimed to investigate the relationship between diabetes and VaMCI among the Chinese, hoping to predict the risk of VaMCI by diabetes and to move the identification of vascular cognitive impairment forward. Methods We collected data from seven clinical centers and nine communities in China. All participants were over 50 years of age and had cognitive complaints. We collected basic information of the participants, and cognitive function was professionally assessed by the Montreal Cognitive Assessment scale. Finally, logistic regression analysis was used to analyze the correlation between each factor and VaMCI. Results A total of 2020 participants were included, including 1140 participants with VaMCI and 880 participants with normal cognition. In univariate logistic regression analysis, age, heavy smoking, and diabetes had a positive correlation with VaMCI. At the same time, being married, high education, and light smoking had a negative correlation with VaMCI. After correction, only diabetes (OR = 1.04, 95% CI: 1.01–1.09, p = 0.05) had a positive correlation with VaMCI, and high education (OR = 0.60, 95% CI:.45–.81, p = 0.001) had a negative correlation with VaMCI. Conclusion In our study, we found that diabetes had a positive correlation with VaMCI, and high education had a negative correlation with VaMCI. Therefore, early identification and timely intervention of diabetes may reduce the risk of VaMCI and achieve early prevention of VaMCI.

Mastitis causes significant losses in the global dairy industry, and the health of animals has been linked to their intestinal microbiota. To better understand the relationship between gastrointestinal microbiota and mastitis in dairy cows, we collected blood, rumen fluid, and fecal samples from 23 dairy cows, including 13 cows with mastitis and 10 healthy cows. Using ELISA kit and high-throughput sequencing, we found that cows with mastitis had higher concentrations of TNF-α, IL-1, and LPS than healthy cows (p 0.05). Principal coordinate analysis (PCOA) revealed significant differences in rumen microbial structure between the two groups (p 0.05), with Aeriscardovia, Lactococcus, and Bacillus as the signature for feces in healthy cows. Furthermore, the results showed distinct microbial interaction patterns in the rumen and feces of cows with mastitis compared to healthy cows. Additionally, we observed correlations between the microbiota in both the rumen and feces of cows and blood inflammatory indicators. Our study sheds new light on the prevention of mastitis in dairy cows by highlighting the relationship between gastrointestinal microbiota and mastitis.

Abstract Background We investigated changes in microR-29c and microR-146a expression in the serum of children with Mycoplasma pneumoniae pneumonia, analysed their relationship with inflammatory factors and disease severity, and evaluated their diagnostic significance. Methods Fifty-six children with Mycoplasma pneumoniae pneumonia were enrolled as the Mycoplasma pneumoniae pneumonia group; 37 healthy children were enrolled as the control group. The microR-29c or microR-146a serum expression levels were determined using real-time quantitative reverse transcription polymerase chain reaction. Interleukin-17, tumour necrosis factor-alpha, and interleukin-1 beta levels were detected using enzyme-linked immunosorbent assay. The correlation between serum microR-29c or microR-146a expression and inflammatory factors was analysed using the Pearson’s method. Receiver operating characteristic curves were used to evaluate the diagnostic value of serum microR-29c, microR-146a, and their combined detection in Mycoplasma pneumoniae pneumonia. Results Compared with that in healthy children, the microR-29c and microR-146a serum levels were significantly downregulated in children with Mycoplasma pneumoniae pneumonia; the decrease was more obvious in children with severe cases than that in those with mild cases. In addition, microR-29c and microR-146a were negatively correlated with increased expression of interleukin-17, tumour necrosis factor-alpha, and interleukin-1 beta. Receiver operating characteristic curves showed that a combination of microR-29c and microR-146a was highly suitable for diagnosing Mycoplasma pneumoniae pneumonia. Conclusion Serum microR-29c and microR-146a were underexpressed in children with Mycoplasma pneumoniae pneumonia, and diagnostic accuracy was significantly improved with combined microR-29c and microR-146a detection. Therefore, both microR-29c and microR-146a levels can be used as biomarkers for the diagnosis of Mycoplasma pneumoniae pneumonia.

Most herbal tea infusions use edible medicinal plants as raw material and are consumed daily. Although herbal tea has a long history of health care applications worldwide, there is a lack of systematic research on the classification and functional nutritional value of various herbal teas. In recent years, the discovery of the rich biological activities and health benefits of herbal tea has become a driving force for researchers interested in its development as a functional food. However, the shortcomings of herbal tea products are not fully known. In particular, the quality evaluation system is not perfect, and there is insufficient data on the mechanism of action of herbal teas in their traditional uses. To better understand the functions and mechanisms of various herbal teas, this article reviews the classification, effective chemical composition, main biological activities, and mechanism of action of herbal teas. Finally, the current advantages, limitations, and direction of future development of herbal tea are determined and discussed.

Water resources are essential for healthy economic growth. Based on data on water consumption and industrial structure, this paper analyzes the evolution trends of the regional water consumption structure and industrial structure in Jinan City, Shandong Province, with the theory of the niche and ecological entropy, which may provide guidance for water resource utilization and social economy development in Jinan City. By establishing a coupling coordination model of the niche and ecological entropy, the dynamic niche evolution of the water consumption structure and industrial structure in Jinan City is analyzed. The results show the following: (1) The niche of agricultural water consumption accounts for the largest portion of the niche of water consumption, with an overall decreasing trend year by year. On the contrary, those of industrial and domestic water consumption have shown slow and fluctuating upward trends, respectively. Similar characteristics and trends are also reflected in the ecological entropy of water consumption. (2) In terms of industrial structure, the niche of the secondary industry accounts for the largest proportion among the three main industries, and only the niche of the tertiary industry is fluctuating upward. (3) Over the years, the overall coupling and coordination degree between the water consumption structure and industrial structure in Jinan was classified as a low degree of imbalance–weak coupling coordination. Among them, the coupling and coordination degree of the tertiary industry is the highest, that of the primary industry is the lowest, and that of the secondary industry is increasing year by year. Thus, it is necessary for Jinan to develop efficient water-saving agriculture and high-tech industry, maintain the development momentum of the tertiary industry, and continue to improve its coupling and coordination status.

In order to solve the problem of insufficient antitumor activity of natural daidzein (DAI),the 4 'and 7 positions of DAI were modified.Firstly,3-(4-chlorophenyl)-7-hydroxy-4H-chromen-4-one was prepared from resorcinol and p-chlorophenylacetic acid by friedel crafts acylation,carbonization and ring closing reaction.The 3-(4-chlorophenyl)-7-\[2-(piperazin-l-yl)ethoxy\]-4H-chrome-4-one (CPEO-43) was synthesized by substitution reaction of 3-(4-Chlorophenyl)-7-hydroxy-4H-chromen-4-one with dibromoethane and piperazine.Column chromatography was used to separate the target product from impurities.Secondly,FT-IR,1H-NMR,13C-NMR and MS were used to identify the compounds.Thirdly,MTT assay was used to detect the inhibitory effect of compound CPEO-43 on A549 lung cancer cells and HCT116 colon cancer cells.Finally,an ultra high performance liquid chromatography (UPLC) method was established for the analysis of CPEO-43 plasma samples.The results show that the structure of the synthesized compound is consistent with that of the target compound confirmed by FT-IR,MS,1H-NMR and 13C-NMR.The inhibitory rates of DAI (10 μmol/L) on A549 lung cancer and HCT116 colon cancer cells are 4.421% and 5.601%,respectively.The inhibitory rates of the same concentration of CPEO-43 on A549 lung cancer cells and HCT116 colon cancer cells are 58.43% and 72.03%,respectively.The IC50 values of CPEO-43 are 2.51 μmol/L and 0.87 μmol/L,respectively.The established UPLC method has no interference from endogenous substances.The linear relationship is good in the range of 0.5~10 μg/mL.The intra-day precision and inter-day precision are less than 10%.The average recoveries of low,medium and high plasma samples of compound CPEO-43 are in the range of 92.98%~100.1%.The antitumor activity of compound CPEO-43 is significantly higher than that of DAI.The established UPLC assay method can easily and rapidly determine the content of CPEO-43 in plasma,and the results are accurate and reliable,provide a theoretical basis for improving the antitumor activity of DAI,and provide method basis for drug clinical testing.

ObjectivesPost-stroke dysphagia is the most common neurological impairment after stroke. The swallowing process is controlled by a network made up of the cerebral cortex, subcortical area, and brainstem structure. The disruption of the swallowing network after stroke leads to dysphagia. The affected swallowing muscles after stroke mainly include the laryngeal muscles (suprahyoid muscle and thyrohyoid muscle) and infrahyoid muscle. These muscles experience kinematic effects and muscle strength weakens, resulting in reduced movement in the swallowing process. Acupuncture can change the excitability of cerebral cortical nerve cells, promote the recovery of neurological function, and enhance neuromuscular excitability, ultimately improving the control of swallowing-related nerves and muscles and promoting swallowing functional recovery. In this meta-analysis, we systematically evaluate the clinical efficacy of acupuncture in the treatment of post-stroke dysphagia.MethodsRandomized controlled trials of tongue acupuncture therapy for post-stroke dysphagia were searched and selected from seven electronic databases (PubMed, CBM, Cochrane, Embase, CNKI, VPCS, and Wan fang). The Cochrane Collaboration tool was used to conduct methodological quality assessment. Rev. Man 5.4 software was utilized to perform data analysis.ResultsA total of 15 studies with 1,094 patients were included. Meta-analysis Showed that WST score WST score (MD = −0.56, 95% CI (−1.23, 0.12), Z = 1.62, p

Abstract Aim The study aimed to prove that both rationality and emotion are indispensable for older people to maintain their ability to live independently during the twilight of their lives. The resilience of older people to dementia were investigated by considering the interactions between educational levels and marriage status. Design A quantitative study was conducted using questionnaires. Methods Four sociodemographic variables (age, sex, educational level and marital status) were collected from 1,177 older Chinese participants, whose mini‐mental state examination scores (MMSE scores) were measured. Results A lower educational level coupled with being widowed caused a greater risk for severe cognitive impairment (relative risk [RR] 1.48; 95% confidence interval [CI] 1.20–1.82; p

This study aims to investigate hydrogeochemical characteristics and groundwater quality in the Hebei Plain and to discuss factors controlling the groundwater quality. A total of 54 groundwater samples were collected and analyzed for 31 hydrogeochemical parameters, and a fuzzy synthetic evaluation (FSE) method was used for assessing groundwater quality. Results show groundwater total hardness, total dissolved solids (TDS), and major ions excluding K+ in phreatic aquifers higher than that in confined aquifers. From the Piedmont plain to the littoral plain, phreatic aquifers towards the reducing environment, and the enhancement of water–rock interaction, ion exchange process, and evaporation probably resulted in the increase in groundwater TDS, major ions (excluding HCO3− and SO42−), B, and Mn concentrations. Moreover, phreatic groundwater chemistry was mainly controlled by rock weathering changing into evaporite dissolution and seawater intrusion from the Piedmont plain to the littoral plain, according to the Gibbs diagram. The proportion of drinkable groundwater in confined aquifers was 1.6 times that in phreatic aquifers. In phreatic aquifers, the proportion of drinkable groundwater in the Piedmont plain was as high as 68%, but none of the drinkable groundwater occurred in the central and littoral plains. Groundwater quality in phreatic aquifers was mainly controlled by five factors, including the water–rock interaction, the marine geogenic sources, the agricultural pollution, the acidification, and the reductive environment. By contrast, groundwater quality in confined aquifers was mainly controlled by three factors, including the water–rock interaction and redox processes, agricultural pollution, and the input of external water. Therefore, in the Hebei Plain, groundwater in confined aquifers is more suitable for drinking purposes than in phreatic aquifers. Additionally, phreatic groundwater in the Piedmont plain should be protected.

Understanding intra-seasonal variation in extreme cold events (ECEs) has important implications for climate prediction and climate adaptation. However, the ECEs difference between early (from December 1 to January 15) and late (from January 16 to February 28) winters is a lack of sufficient understanding. Herein, we investigated the trends of ECEs over eastern China in early and late winters. Results showed that the number of days with ECEs had a faster and uniformly decreasing trend in late winter over eastern China, whereas the decreasing trend in early winter was not significant because of the dipole pattern with an increase of ECEs in northeast China and a decrease of ECEs in southeast China during the time period 1980–2021. This denoted that China was presenting a pattern of “cold early winter–warm late winter”. The feature of cold early winter was related to a significant increase in high-latitude blocking highs extending poleward and reaching the Arctic Circle in early winter during the last 20 years. In particular, there was a large-scale tilted high ridge from the Ural Mountains to northern Asia, which favored the negative phase of the Arctic oscillation. This, in turn, led to a strong Siberian high and East Asian winter monsoon. Strong cold advection related to the circulation anomalies caused an ECEs increase in northeast China and dominated the change in temperature over eastern China in early winter. By contrast, the decrease in ECEs in late winter in the last 20 years was more related to the interdecadal enhancement of the anticyclonic anomaly over the north Pacific (NPAC). The strong NPAC extended to East Asia in a zonal direction, causing strong warm anomalies in eastern China through warm advection and diabatic heating, which weakened the northerly and prevented the East Asian trough from moving south, resulting in a warmer East Asia and a uniform decrease in late winter.

As the source of the Jumahe River, the Juma springs has received the attention of many experts and scholars. Previous studies focused mainly on the chemical and dynamic characteristics of the groundwater and the spring discharge characteristics, the hydrogen and oxygen isotopes characteristics were seldom examined, and the explanation of the causes of the Beihai springs was only a rough qualitative overview. To illustrate the characteristics of hydrogen and oxygen isotopes of the groundwater in the northern Laiyuan Basin and reveal the forming mode of the Beihai springs in detail, groundwater samples in different aquifers were systematically collected for the first time in the study are area and the hydrogen and oxygen isotopic components were determined in this paper. The results show that the values of δD and δ18O in the water samples fall on or near the regional meteoric water line, indicating that the meteoric water is the main recharge source of the groundwater. The altitude effect is obvious and the groundwater flow path is long for the groundwater in the dolomite aquifer and limestone aquifer, the groundwater flow path is short and the evaporation effect is strong for the groundwater in the unconsolidated sediment aquifer. The deuterium excess of the sample in the dolomite aquifer, limestone aquifer and unconsolidated sediment aquifer are 6.0‰−11.6‰, 4.2‰−11.2‰ and 3.8‰−8.0‰, respectively, much smaller than that in the meteoric water, showing that the karst water and the pore water undergo different processes. When the groundwater in the dolomite aquifer and limestone aquifer flows from the recharge areas to the discharge areas, hydraulic connection and mixing occur in the fault zone near Xiaoxizhuang and Xianglutun. Groundwater rises during the process of flow to the center of the basin because of the resistance effect of the fault, and groundwater in the dolomite aquifer and limestone aquifer is subsequently recharged by the pore water in the unconsolidated sediment aquifer, at last the groundwater emerges on the ground surface in the form of springs as the Beihai springs. Before the pore water joins in, the average recharge comes from the limestone aquifer and dolostone aquifer is about 48.4%−57.6% and 42.4%−51.6%, respectively.

The Fuyou (Fy) formula is an in-hospital preparation consisting of traditional Chinese medicine (TCM) that has been used for treating precocious puberty (PP) for more than 20 years. In this study, we aimed to clarify the effect of the Fy formula and its major components on PP. To confirm the effect of the Fy formula on the release of hypothalamic gonadotropin-releasing hormone (GnRH), GT1-7 cells were treated with estrogen to build the model group and subsequently treated with the Fy formula and its major components to explore their effects on the secretion of GnRH. The level of GnRH in GT1-7 cells was determined using enzyme-linked immunosorbent assay. The results illustrated that, compared to the model group, the Fy formula inhibited the release of GnRH. In addition, the expression levels of proteins related to GnRH secretion, including GnRH, gonadotropin-releasing hormone receptor (GnRHR), Kiss-1 metastasis-suppressor (Kiss1), G-protein coupled receptor 54 (GPR54), estrogen receptor α (ERα), insulin-like growth factor-1 (IGF-1), and insulin-like growth factor-1 receptor (IGF-1R), were detected by real-time polymerase chain reaction (RT-qPCR). The results demonstrated that the Fy formula significantly reduced the level of GnRH secretion in the GT1-7 cell lines compared with the model group. Moreover, it significantly downregulated the expression of GnRH, GnRHR, Kiss1, GPR54, ERα, IGF-1, and IGF-1R. In summary, our results indicate that the Fy formula and its major components may inhibit the effects of estrogen, which alleviates PP through transcriptional regulation of target genes.

Nowadays, Heart disease is one of the crucial impacts of mortality in the country. In clinical data analysis, predicting cardiovascular disease is a primary challenge. Deep learning (DL) has been demonstrated to be effective in helping to determine and forecast a huge amount of data produced by the health industry. In this paper, the proposed Recursion enhanced random forest with an improved linear model (RFRF-ILM) to detect heart disease. This paper aims to find the key features of the prediction of cardiovascular diseases through the use of machine learning techniques. The prediction model is adding various combinations of features and various established methods of classification. it produces a better level of performance with precision through the heart disease prediction model. In this study, the factors leading to cardiovascular disease can be diagnosed. A comparison of important variables showed with the Internet of Medical Things (IoMT) platform, for data analysis. This indicates that coronary artery disease develops more often in older ages. Also important in this disease's outbreak is high blood pressure. For this purpose, measures must be taken to prevent this disease and Diabetes provides a further aspect that should be taken into consideration in the occurrence of coronary artery disease with 96.6 % accuracy,96.8% stability ratio and 96.7% F-measure ratio.

Introduction. Obstructive sleep apnea syndrome (OSAS) is related to hypertension. Vascular remodeling is both the pathogenesis and the structural change basis of OSAS-related hypertension. Exploring miRNA functioning in OSAS-related hypertension may offer novel diagnostic and therapeutic targets for controlling hypertension-associated cardiovascular diseases. However, the role of miR-199a-5p in OSAS-related hypertension has not been demonstrated yet. Methods. In this study, we investigated the role of miR-199a-5p and HIF-1α in OSAS-related hypertension by performing in vitro cell experiments and in vivo animal experiments. Rat aortic smooth muscle cells (A7r5) were cultured under hypoxia as an in vitro model. To establish the animal model of OSAS-related hypertension, the rats were under exposure to chronic intermittent hypoxia (CIH) in a hypoxic instrument. The rats were randomly grouped into normal, CIH, CIH+NC, and CIH+miR-199a-5p. Results. By establishing an animal model, we found decreased miR-199a-5p expression and increased HIF-1α expression in OSAS with hypertension. The overexpressed miR-199a-5p could reduce systolic blood pressure and relieve oxidase stress and inflammation. miR-199a-5p treatment could overturn the upregulation of HIF-1α and TGF-β1 and downregulation of α-SMA. Overexpressed miR-199a-5p might attenuate vascular remodeling through HIF-1α downregulation. miR-199a-5p/HIF-1α may inhibit proliferation of vascular smooth muscle cells under hypoxia. Conclusion. miR-199a-5p may relieve OSAS-related hypertension by targeting HIF-1α and be a novel potential therapeutic target.

Objectives: The incidence of psychological and behavioral problems and depression among adolescents is increasing year by year, which has become an important public health problem. Alexithymia, as an important susceptible factor of adolescent depression, may continue to develop and strengthen under the stimulation of COVID-19-related stressors. However, no studies have focused on alexithymia in adolescent depression during the pandemic in China. This study aims to investigate the incidence and related factors of alexithymia in adolescent depression during the pandemic.Methods: Three hundred adolescent patients were enrolled from October 2020 to May 2021. The general demographic information of all participants was collected, and the clinical characteristics were assessed by the 20-item Toronto Alexithymia Scale (TAS-20), the Adolescent Self-Rating Life Events Check (ASLEC) List, the Childhood Trauma Questionnaire (CTQ), and the Positive and Negative Suicide Ideation (PANSI) Inventory.Results: The incidence of alexithymia was significantly higher among adolescents with depression (76.45%) during the pandemic. There were significant differences in school bullying, disease severity, ASLEC score, CTQ score and PANSI score between adolescents with and without alexithymia. In addition, learning stress, health and adaptation problems during the pandemic may be influential factors in alexithymia of adolescent depression (P < 0.05).Conclusions: According to the results, we found a high incidence of alexithymia in adolescent depression during the pandemic. More support and attention from families, schools and society is needed to develop preventive and targeted psychological interventions as early as possible.

A large number of studies have revealed that epigenetics plays an important role in cancer development. However, the currently-developed epigenetic drugs cannot achieve a stable curative effect. Thus, it may be necessary to redefine the role of epigenetics in cancer development. It has been shown that embryonic development and tumor development share significant similarities in terms of biological behavior and molecular expression patterns, and epigenetics may be the link between them. Cell differentiation is likely a manifestation of epigenetic homeostasis at the cellular level. In this article, we introduced the importance of epigenetic homeostasis in cancer development and analyzed the shortcomings of current epigenetic treatment regimens. Understanding the dynamic process of epigenetic homeostasis in organ development can help us characterize cancer according to its differentiation stages, explore new targets for cancer treatment, and improve the clinical prognosis of patients with cancer.

Linear and star block copolymer (BCP) nanoparticles of (polystyrene-block-poly(4-vinylpyridine))n (PS-b-P4VP)n with arm numbers of 1, 2, 3, and 4 were prepared by two methods of polymerization-induced self-assembly (PISA) and general self-assembly of block copolymers in the low-polar organic solvent, toluene. The effect of the arm number on the size and/or morphology of the (PS-b-P4VP)n nanoassemblies synthesized by the two methods in toluene and on the polymerization kinetics was investigated in detail. Our results show that in toluene, a low-polar solvent, the topology not only affected the morphology of the BCP nanoparticles prepared by PISA, but also influenced the BCP nanoparticles synthesized through general self-assembly.

Anesthesia signal monitoring is a very important indicator in surgery, and the effective monitoring of anesthesia depth has been the goal of anesthesiologists and biomedical engineering experts in recent decades. First, the wavelet transform method is used to analyze the anesthesia monitoring EEG signals, and the extracted features are clustered by wavelet classifier to estimate the depth of anesthesia. Second, the characteristics of eigenvectors are constructed by a singular value decomposition based on wavelet transform coefficients. The extraction method extracts the characteristics of the mid-latency auditory evoked EEG under anesthesia. Finally, this paper collected a large amount of clinical data and established a clinical database of anesthesia depth. The experimental results show the effectiveness of the method.

This study was aimed to estimate the relative bioavailability (RBA) of l-methionine (l-Met) to dl-methionine (dl-Met) in broiler chickens from 1 to 21 d of age. A total of 1664 one-day-old broiler chicks were randomly divided into 13 groups with 8 replicates per group. The basal diet contained 60% of the NRC requirement for Met. Birds in treatment groups were fed the basal diet supplemented with graded levels of dl- or l-Met, resulting in the dietary content of Met at 75%, 80%, 85%, 90%, 95% and 100% of the NRC requirement, respectively. Results showed that there were linear increases (p

Fu-you formula (FY), a Traditional Chinese Medicine (TCM) formula composed of 12 herbs, as an in-hospital preparation, has been used treat to precocious puberty (PP) for decades. However, the lack of phytochemical characterization and mechanism of FY remains the main limitation for its spreading. In this study, we analyze the components and mechanisms of FY in PP, based on the integrated pharmacology. Investigated main constituents, targets, pathways of FY by using an integrative pharmacology, and recognized main constituents by HPLC-MS/MS. Then, observed the levels of Follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estrogen (E2) in danazol-induced PP in Sprague–Dawley (SD) rats. Lastly, retrospective study analyzed the clinical data of 575 patients who were diagnosed PP, treated by the FY, and followed-up in our hospital from 2014–2020. The result that total of 116 important candidate targets were selected based on pharmacological analysis. Selected the top 10 values key targets such as the estrogen receptor alpha (ESR1) and insulin-like growth factor 1 (IGF1), were localized and the related gene functions were determined. Gene functions were associated with biological regulation, a cellular process, or signaling pathway, such as the Estrogen signaling pathway, MAPK signaling pathway and PI3K-Akt signaling pathway. By recognizing the five compounds related to the ESR1 and IGF1, which are Quercetin, kaempferol, Luteolin, Apigenin, and Emodin. The results of the molecular docking study further showed that the flavonoids had a strong binding affinity for ESR1 and IGF1 after docking into the crystal structure. The results showed that the FY could effectively reduce E2, LH, and FSH levels in SD rats. Furthermore, the results of the retrospective analysis of medical records showed that the FY could remarkably reduce E2 levels in girls with PP.

Objectives: Healthcare workers (HWs) experienced high levels of work stress during the COVID-19 pandemic, leading to a high risk of sleep disturbances. This meta-analysis aimed to explore the prevalence of sleep disturbances and sleep quality in Chinese HWs during the COVID-19 pandemic.Methods: English (PubMed, EMBASE, PsycINFO, Web of Science, and the Cochrane Library) and Chinese databases (WanFang, Chinese National Knowledge Infrastructure, and SinoMed) were systematically and independently searched for relevant studies published from December 1, 2019, to May 20, 2020. The pooled prevalence of sleep disturbances and sleep quality were calculated using a random-effects model.Results: A total of 17 studies involving 12,682 Chinese HWs were included in the meta-analysis. The pooled prevalence of sleep disturbances in Chinese HWs was 45.1% (95% CI: 37.2–53.1%). We found that the prevalence of sleep disturbances varied among frontline, infected, and non-frontline HWs (Q = 96.96, p < 0.001); females and males (Q = 9.10, p = 0.003); studies using different assessment instruments (Q = 96.05, p < 0.001); and studies with different sample sizes (Q = 5.77, p = 0.016) and cut-off values (Q = 62.28, p < 0.001). The pooled mean total score of the Pittsburgh Sleep Quality Index (PSQI) was 9.83 (95% CI: 8.61–11.04). HWs in Wuhan had a higher total PSQI score than those in other regions (Q = 9.21, p = 0.002).Conclusion: Sleep disturbances were common in Chinese HWs during the COVID-19 pandemic, particularly in frontline and infected HWs. Our results indicate the heavy mental health burden on HWs during the COVID-19 pandemic in China and can provide other countries with valuable information to assist HWs during the crisis.

The microbiota in raw milk plays an important role in the health of dairy cows and the safety of dairy products, which might be influenced by that in teat skin. However, the microbiota composition in raw milk and teat skin, as well as the bacterial interaction between the two adjacent spatial locations, remains elusive. Here, we investigated the composition, diversity, and co-occurrence network of the bacterial communities in raw milk and on teat skin, as well as the shift of bacterial communities during the teat bath using 469 samples from 156 individual cows. We observed that raw milk and teat skin harbored significantly different bacterial communities according to an assessment of the genera numbers (p < 0.05) and PCoA analysis (ANOSIM p < 0.05). The microbiota in raw milk was dominated by Proteobacteria (58.5% in relative abundance) at the phylum level and by Pseudomonas (51.2%) at the genus level, while that in teat skin was dominated by Firmicutes (46.9%) at the phylum level and by Pseudomonas (11.0%) at the genus level. We observed a massive difference between the bacterial subnetworks in raw milk and teat, and the bacterial abundance in these two adjacent spatial locations was positively correlated (p < 0.05). Using Bayesian algorithms, we identified that 92.1% of bacteria in raw milk were transferred from teat skin, while 63.6% of bacteria on teat skin were transferred from raw milk. Moreover, microbiota composition in teat skin could be affected by the teat bath with iodine disinfectant, which tended to be more similar to that in raw milk after the teat bath (p < 0.05), while the abundance of the dominant genus Pseudomonas significantly increased (p < 0.05). These findings expand our knowledge on the microbiota composition in teat skin and raw milk, as well as the interaction between these two adjacent spatial locations.

Rationale. Hemoptysis is a rare but often life-threatening condition in pediatric patients. Massive hemoptysis can easily lead to asphyxia, respiratory failure, shock, and even death. The most common causes of severe hemoptysis are lower respiratory tract infection, vascular malformation, and bronchial foreign body. We present an unusual case of massive hemoptysis caused by malformation of the bronchial artery, which includes bronchial artery hypertrophy, bronchial-pulmonary artery fistula, and ectopic bronchial artery. Patient. An 11-year-old boy was admitted to the hospital with mild hemoptysis lasting for the two preceding days. He did not report any discomfort, such as fever or chest pain. His complete blood count and coagulation function were normal. Chest X-ray documented lower right pneumonia. Massive hemoptysis occurred on the night of the admission. Diagnosis. Bronchial arteriography revealed that the right lower bronchial artery and the ectopic bronchial artery from the renal artery were the responsible vessels for hemoptysis. Interventions. The boy underwent a successful bronchial artery embolization and bronchoscopy to remove the blood clot from the airway. Outcomes. After bronchial artery embolization and bronchoscopy, the boy recovered without complications. Hemoptysis and chest pain disappeared, and chest radiographs returned to normal. Lessons. Bronchial arterial bleeding often presents as life-threatening massive hemoptysis. Patients should immediately receive hemostatic treatment and undergo chest CTA, bronchial arteriography, BAE, and bronchoscopy according to their condition. Rapid identification of the etiology and symptomatic treatment are critical to saving the lives of children.

Due to the remarkable anti-tumor activities of oridonin (Ori), research on Rabdosia rubescens has attracted more and more attention in the pharmaceutical field. The purpose of this study was to extract Ori from R. rubescens by ultrasound-assisted extraction (UAE) and prepare Ori liposomes as a novel delivery system to improve the bioavailability and biocompatibility. Response surface methodology (RSM), namely Box-Behnken design (BBD), was applied to optimize extraction conditions, formulation, and preparation process. The results demonstrated that the optimal extraction conditions were an ethanol concentration of 75.9%, an extraction time of 35.7 min, and a solid/liquid ratio of 1:32.6. Under these optimal conditions, the extraction yield of Ori was 4.23 mg/g, which was well matched with the predicted value (4.28 mg/g). The optimal preparation conditions of Ori liposomes by RSM, with an ultrasonic time of 41.1 min, a soybean phospholipids/drug ratio of 9.6 g/g, and a water bath temperature of 53.4 °C, had higher encapsulation efficiency (84.1%). The characterization studies indicated that Ori liposomes had well-dispersible spherical shapes and uniform sizes with a particle size of 137.7 nm, a polydispersity index (PDI) of 0.216, and zeta potential of −24.0 mV. In addition, Ori liposomes presented better activity than free Ori. Therefore, the results indicated that Ori liposomes could enhance the bioactivity of Ori, being proposed as a promising vehicle for drug delivery.

To better understand the transition of rumen function during the weaning period in dairy calves, sixteen Holstein dairy calves were selected and divided into two groups: pre-weaning (age = 56 ± 7 day, n = 8) and post-weaning (age = 80 ± 6 day, n = 8). The rumen fluid was obtained by an oral gastric tube. The rumen fermentation profile, enzyme activity, bacteria composition, and their inter-relationship were investigated. The results indicated that the post-weaning calves had a higher rumen acetate, propionate, butyrate, and microbial crude protein (MCP) than the pre-weaning calves (p < 0.05). The rumen pH in the post-weaning calves was lower than the pre-weaning calves (p < 0.05). The protease, carboxymethyl cellulase, cellobiohydrolase, and glucosidase in the post-weaning calves had a lower trend than the pre-weaning calves (0.05 < p < 0.1). There was no difference in α and β diversity between the two groups. Linear discriminant analysis showed that the phylum of Fibrobacteres in the post-weaning group was higher than the pre-weaning group. At the genus level, Shuttleworthia, Rikenellaceae, Fibrobacter, and Syntrophococcus could be worked as the unique bacteria in the post-weaning group. The rumen bacteria network node degree in the post-weaning group was higher than the pre-weaning group (16.54 vs. 9.5). The Shuttleworthia genus was highly positively correlated with MCP, propionate, total volatile fatty acid, glucosidase, acetate, and butyrate (r > 0.65, and p < 0.01). Our study provided new information about the rumen enzyme activity and its relationship with bacteria, which help us to better understand the effects of weaning on the rumen function.

We presented an extended-wavelength InGaAsSb infrared unipolar barrier detector working at room temperature. The detector with GaSb lattice-matched InGaAsSb absorb layer and AlGaAsSb unipolar barrier can achieve high material quality and low dark current. The dark current density was 2.29×10-5 A/cm2 at 0 bias at 77K. At room temperature the dark current at 0 bias was 4×10-3 A/cm2 and the R0A is high to 44 Ω · cm2. We fabricated the cone arrays in the InGaAsSb absorb layer to reduce the reflection of the radiation and extend the spectrum response to visible area. The extended-wavelength detector had the response from the wavelength of 0.4 μm. Further experiment showed the cone arrays also reduced the dark current of the detector at room temperature.

Antibiotically disturbed gastrointestinal microbiota needs a long period time to be restored to normal, which may cause a series of problems to the host. The understanding of restoration of the biased microbiota by antibiotics remains largely unknown. Here, we investigated the microbiota shift in foregut (rumen) and hindgut (rectum) of lactating cows after antibiotics exposure as well as after antibiotics withdrawal with (Microbiota transplantation, MT group) or without (Control, CON group) microbiota transplantation. We were able to demonstrate that microbiota in both foregut and hindgut significantly changed after 3 or 14 days of antibiotics exposure, and the changes persisted over long period of time (>18 days) after withdrawing the antibiotics. We further observed a faster restoration of microbiota in both foregut and hindgut of MT group than CON group, microbiota in foregut was mainly benefited from microbiota transplantation by restoring the alpha-diversity as well as within-group similarity, while microbiota in hindgut was primarily benefited from microbiota transplantation by reestablishing the co-occurrence network (nodes number, edges number, density, modularity as well as closeness centrality). These results together expanded our understanding of restoration of the biased microbiota by antibiotics, and may also be instructive to deal with the delayed microbiota restoration at least in cows.

Directed forgetting (DF) assists in preventing outdated information from interfering with cognitive processing. Previous studies pointed that self-referential items alleviated DF effects due to the elaboration of encoding processes. However, the retrieval mechanism of this phenomenon remains unknown. Based on the dual-process framework of recognition, the retrieval of self-referential information was involved in familiarity and recollection. Using source memory tasks combined with event-related potential (ERP) recording, our research investigated the retrieval processes of alleviative DF effects elicited by self-referential information. The FN400 (frontal negativity at 400 ms) is a frontal potential at 300–500 ms related to familiarity and the late positive complex (LPC) is a later parietal potential at 500–800 ms related to recollection. The FN400 effects of source memory suggested that familiarity processes were promoted by self-referential effects without the modulation of to-be-forgotten (TBF) instruction. The ERP results of DF effects were involved with LPCs of source memory, which indexed retrieval processing of recollection. The other-referential source memory of TBF instruction caused the absence of LPC effects, while the self-referential source memory of TBF instruction still elicited the significant LPC effects. Therefore, our neural findings suggested that self-referential processing improved both familiarity and recollection. Furthermore, the self-referential processing advantage which was caused by the autobiographical retrieval alleviated retrieval inhibition of DF, supporting that the self-referential source memory alleviated DF effects.

Retrieval inhibition hypothesis of directed forgetting effects assumed TBF (to-be-forgotten) items were not retrieved intentionally, while selective rehearsal hypothesis assumed the memory representation of retrieved TBF (to-be-forgotten) items was weaker than TBR (to-be-remembered) items. Previous studies indicated that directed forgetting effects of item-cueing method resulted from selective rehearsal at encoding, but the mechanism of retrieval inhibition that affected directed forgetting of TBF (to-be-forgotten) items was not clear. Strategic retrieval is a control process allowing the selective retrieval of target information, which includes retrieval orientation and strategic recollection. Retrieval orientation via the comparison of tasks refers to the specific form of processing resulted by retrieval efforts. Strategic recollection is the type of strategies to recollect studied items for the retrieval success of targets. Using a “directed forgetting” paradigm combined with a memory exclusion task, our investigation of strategic retrieval in directed forgetting assisted to explore how retrieval inhibition played a role on directed forgetting effects. When TBF items were targeted, retrieval orientation showed more positive ERPs to new items, indicating that TBF items demanded more retrieval efforts. The results of strategic recollection indicated that: (a) when TBR items were retrieval targets, late parietal old/new effects were only evoked by TBR items but not TBF items, indicating the retrieval inhibition of TBF items; (b) when TBF items were retrieval targets, the late parietal old/new effect were evoked by both TBR items and TBF items, indicating that strategic retrieval could overcome retrieval inhibition of TBF items. These findings suggested the modulation of strategic retrieval on retrieval inhibition of directed forgetting, supporting that directed forgetting effects were not only caused by selective rehearsal, but also retrieval inhibition.

Although many behavioral studies have reported associative memory was different from item memory, evidence coming from ERP researches has been in debate. In addition, directed forgetting effect for items has been fully discussed, but whether association between items can be directed-forgotten was unclear. The directed forgetting effect was important for disassociating the item retrieval and associative retrieval because of the one-to-one mapping relationship both between item retrieval and familiarity and between associative retrieval and recollection Thus, the aim of this study was to investigate the dissociation between item retrieval and associative retrieval and test directed forgetting effect for associative information. Associative recognition paradigm combined with directed forgetting paradigm by ERP recording was employed. Old/rearranged effect in to-be-remembered condition, which was associated with associative memory, was significant at 500-800 ms (LPC) but not at 300-500 ms interval (FN400), indicating that item information was retrieved prior to associative information. The ERP wave calculated by subtracting the to-be-forgotten old pairs with old response from those with rearranged response, which reflected associative retrieval in the to-be-forgotten condition, was negative from 500 to 800 ms (reversal old/new effect), indicating that association between items can be directed-forgotten. Similar evidence was obtained by contrasting rearranged responses aimed to the to-be-forgotten old pairs with those aimed to the to-be-remembered rearranged pairs, which actually represented the complete failure of associative retrieval. Therefore, item retrieval and associative retrieval were indexed by FN400 and LPC respectively, with associative retrieval more inhibited than item retrieval.

The interaction between recognition memory and emotion has become a research hotspot in recent years. Dual process theory posits that familiarity and recollection are two separate processes contributing to recognition memory, but further experimental evidence is needed. The present study explored the emotional context effects on successful and unsuccessful source retrieval amongst 15 high-trait-anxiety college students by using event-related potentials (ERPs) measurement. During study, a happy, fearful, or neutral face picture firstly was displayed, then a Chinese word was superimposed centrally on the picture and subjects were asked to remember the word and the corresponding type of picture. During test participants were instructed to press one of four buttons to indicate whether displayed word was an old or new word? And then, for old word, indicate whether it had been shown with a fearful, happy or neutral face during study. ERPs were generally more positive for remembered words than for new words and the ERP difference was termed as an old/new effect. It was found that, for successful source retrieval (it meaned both item and source were remembered accurately) between 500 and 700ms (corresponding to a late positive component, LPC), there were significant old/new effects in all contexts. However, for unsuccessful source retrieval (it meaned the correct recognition of old items matched with incorrect source attribution), there were no significant old/new effects in happy and neutral contexts, though significant old/ new effects were observed in the fearful context. Between 700 and 1200ms (corresponding to a late slow wave, LSW), there were significant old/new effects for successful source retrieval in happy and neutral contexts. However, in the fearful context, the old/new effects were reversed, ERPs were more negative for successful source retrieval compared to correct rejections. Moreover, there were significant emotion effects for successful source retrieval at this time window. Further analysis showed ERPs of old items were more negative in fearful context than in neutral context. The results showed that early unsuccessful fearful source retrieval processes (related to familiarity) were enhanced, but late successful fearful source retrieval processes during source retrieval monitoring (related to recollection) were weakened. This provided preliminary

The passion fruit (Passiflora edulis Sims), also known as the purple granadilla, is widely cultivated as the new darling of the fruit market throughout southern China. This exotic and perennial climber is adapted to warm and humid climates, and thus is generally intolerant of cold. There is limited information about gene regulation and signaling pathways related to the cold stress response in this species. In this study, two transcriptome libraries (KEDU_AP vs. GX_AP) were constructed from the aerial parts of cold-tolerant and cold-susceptible varieties of P. edulis, respectively. Overall, 126,284,018 clean reads were obtained, and 86,880 unigenes with a mean size of 1449 bp were assembled. Of these, there were 64,067 (73.74%) unigenes with significant similarity to publicly available plant protein sequences. Expression profiles were generated, and 3045 genes were found to be significantly differentially expressed between the KEDU_AP and GX_AP libraries, including 1075 (35.3%) up-regulated and 1970 (64.7%) down-regulated. These included 36 genes in enriched pathways of plant hormone signal transduction, and 56 genes encoding putative transcription factors. Six genes involved in the ICE1–CBF–COR pathway were induced in the cold-tolerant variety, and their expression levels were further verified using quantitative real-time PCR. This report is the first to identify genes and signaling pathways involved in cold tolerance using high-throughput transcriptome sequencing in P. edulis. These findings may provide useful insights into the molecular mechanisms regulating cold tolerance and genetic breeding in Passiflora spp.

The vast majority of decision-making research is performed under the assumption of the value maximizing principle. This principle implies that when making decisions, individuals try to optimize outcomes on the basis of cold mathematical equations. However, decisions are emotion-laden rather than cool and analytic when they tap into life-threatening considerations. Using functional magnetic resonance imaging (fMRI), this study investigated the neural mechanisms underlying vital loss decisions. Participants were asked to make a forced choice between two losses across three conditions: both losses are trivial (trivial-trivial), both losses are vital (vital-vital), or one loss is trivial and the other is vital (vital-trivial). Our results revealed that the amygdala was more active and correlated positively with self-reported negative emotion associated with choice during vital-vital loss decisions, when compared to trivial-trivial loss decisions. The rostral anterior cingulate cortex was also more active and correlated positively with self-reported difficulty of choice during vital-vital loss decisions. Compared to the activity observed during trivial-trivial loss decisions, the orbitofrontal cortex and ventral striatum were more active and correlated positively with self-reported positive emotion of choice during vital-trivial loss decisions. Our findings suggest that vital loss decisions involve emotions and cannot be adequately captured by cold computation of minimizing losses. This research will shed light on how people make vital loss decisions.

Taohong Siwu Decoction (THSWD), a traditional Chinese prescription renowned for its efficacy in promoting blood circulation and alleviating blood stasis, was investigated in this study to delineate its potential active components and discern the core targets (CTs) and signaling pathways implicated in the treatment of Alzheimer's disease (AD). Initially, 25 active compounds (ACs) and 478 corresponding active ingredient targets (AITs) of THSWD were meticulously identified by scrutinizing the active ingredients and their targets through the Traditional Chinese Medicine Systems Pharmacology (TCMSP) and Similarity Ensemble Approach (SEA) databases. Subsequently, a comprehensive compilation of 724 AD-related targets was assembled from the Therapeutic Target Database (TTD), DisGeNET, DrugBank, Genetic Association Database (GAD), and GeneCards databases. Through a meticulous alignment of AITs with disease-related targets, 64 overlapping targets (OTs) emerged as critical intersections. To distill the key compounds, an intricate analysis of the interrelationships among the 25 ACs and 64 OTs resulted in the identification of 21 first-level key compounds (FKCs). Further scrutiny through protein-protein interaction (PPI) analysis of the 64 OTs revealed 33 CTs. KEGG cluster analysis of these CTs yielded the top 73 pathways, forming the basis for constructing a network diagram encompassing "21 FKCs-33 CTs-73 pathways" using Cytoscape 3.7.2 software. Refining the network through the selection of topological parameters led to the identification of 19 second key components (SKCs). This information was then employed to construct a refined network, "19 SKCs-33 CTs-73 pathways", providing deeper insights into the intricate connections within the system. Further analyses culminated in the creation of a comprehensive network map, encapsulating "6 single drugs-8 potential active ingredients-13 core targets-54 signaling pathways", elucidating the multifaceted intervention of THSWD in AD. These results offered a novel perspective for understanding the pharmacodynamic material basis of THSWD and paved the way for in-depth investigations into its mechanisms of action and clinical applications in the context of AD. [ABSTRACT FROM AUTHOR]