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21. A hybrid microfluidic-vacuum device for direct interfacing with conventional cell culture methods

Author

Chung, Bong Geun, Park, Jeong Won, Hu, Jia Sheng, Huang, Carlos, Monuki, Edwin S, and Jeon, Noo

Subjects
polymorphonuclear leukocytes, neutrophil chemotaxis, hydrogen-peroxide, gradient, generation, growth, induction, motility, biology
Abstract

BackgroundMicrofluidics is an enabling technology with a number of advantages over traditional tissue culture methods when precise control of cellular microenvironment is required. However, there are a number of practical and technical limitations that impede wider implementation in routine biomedical research. Specialized equipment and protocols required for fabrication and setting up microfluidic experiments present hurdles for routine use by most biology laboratories.ResultsWe have developed and validated a novel microfluidic device that can directly interface with conventional tissue culture methods to generate and maintain controlled soluble environments in a Petri dish. It incorporates separate sets of fluidic channels and vacuum networks on a single device that allows reversible application of microfluidic gradients onto wet cell culture surfaces. Stable, precise concentration gradients of soluble factors were generated using simple microfluidic channels that were attached to a perfusion system. We successfully demonstrated real-time optical live/dead cell imaging of neural stem cells exposed to a hydrogen peroxide gradient and chemotaxis of metastatic breast cancer cells in a growth factor gradient.ConclusionThis paper describes the design and application of a versatile microfluidic device that can directly interface with conventional cell culture methods. This platform provides a simple yet versatile tool for incorporating the advantages of a microfluidic approach to biological assays without changing established tissue culture protocols.

Published
2007

32. Additional file 1 of Effect of gut microbiome-derived metabolites and extracellular vesicles on hepatocyte functions in a gut-liver axis chip

Author

Kang, Seong Goo, Choi, Yoon Young, Mo, Sung Jun, Kim, Tae Hyeon, Ha, Jang Ho, Hong, Dong Ki, Lee, Hayera, Park, Soo Dong, Shim, Jae-Jung, Lee, Jung-Lyoul, and Chung, Bong Geun

Abstract

Additional file 1.Table S1. Geometric parameters of the 3D computational fluid dynamics model. Figure S1.Comparison graph according to (A) pore size and (B) porosity of membranes. Figure S2. Effects of the fluidic flow onviability in hepG2 spheroids.

Published
2023
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50. Antioxidant, Enzyme, and H 2 O 2 -Triggered Melanoma Targeted Mesoporous Organo-Silica Nanocomposites for Synergistic Cancer Therapy.

Author

Choi, Hyung Woo, Lim, Jae Hyun, Kang, Taewook, and Chung, Bong Geun

Subjects
DACARBAZINE, CANCER treatment, DOXORUBICIN, CONTROLLED release drugs, NANOCOMPOSITE materials, DRUG delivery systems, PHOTODYNAMIC therapy
Abstract

The multi-stimuli responsive drug delivery system has recently attracted attention in cancer treatments, since it can reduce several side effects and enhance cancer therapeutic efficacy. Herein, we present the intracellular antioxidant (glutathione, GSH), enzyme (hyaluronidase, HAase), and hydrogen peroxide (H2O2) triggered mesoporous organo-silica (MOS) nanocomposites for multi-modal treatments via chemo-, photothermal, and photodynamic cancer therapies. A MOS nanoparticle was synthesized by two-types of precursors, tetraethyl orthosilicate (TEOS) and bis[3-(triethoxysilyl)propyl] tetrasulfide (BTES), providing large-sized mesopores and disulfide bonds cleavable by GSH. Additionally, we introduced a new β-cyclodextrin-hyaluronic acid (CDHA) gatekeeper system, enabling nanocomposites to form the specific interaction with the ferrocene (Fc) molecule, control the drug release by the HAase and H2O2 environment, as well as provide the targeting ability against the CD44-overexpressing melanoma (B16F10) cells. Indocyanine green (ICG) and doxorubicin (Dox) were loaded in the MOS-Fc-CDHA (ID@MOS-Fc-CDHA) nanocomposites, allowing for hyperthermia and cytotoxic reactive oxygen species (ROS) under an 808 nm NIR laser irradiation. Therefore, we demonstrated that the ID@MOS-Fc-CDHA nanocomposites were internalized to the B16F10 cells via the CD44 receptor-mediated endocytosis, showing the controlled drug release by GSH, HAase, and H2O2 to enhance the cancer therapeutic efficacy via the synergistic chemo-, photothermal, and photodynamic therapy effect. [ABSTRACT FROM AUTHOR]

Published
2022
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