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Abstract. Background:. Hyperbaric oxygen treatment (HBOT) has been demonstrated to influence the keloid recurrence rate after surgery and to relieve keloid symptoms and other pathological processes in keloids. To explore the mechanism of the effect of HBOT on keloids, tumor immune gene expression and immune cell infiltration were studied in this work. Methods:. From February 2021 to April 2021, HBOT was carried out on keloid patients four times before surgery. Keloid tissue samples were collected and divided into an HBOT group (keloid with HBOT before surgery [HK] group, n = 6) and a non-HBOT group (K group, n = 6). Tumor gene expression was analyzed with an Oncomine Immune Response Research Assay kit. Data were mined with R package. The differentially expressed genes between the groups were compared. Hub genes between the groups were determined and verified with Quantitative Real-time PCR. Immune cell infiltration was analyzed based on CIBERSORT deconvolution algorithm analysis of gene expression and verified with immunohistochemistry (IHC). Results:. Inflammatory cell infiltration was reduced in the HK group. There were 178 upregulated genes and 217 downregulated genes. Ten hub genes were identified, including Integrin Subunit Alpha M (ITGAM), interleukin (IL)-4, IL-6, IL-2, Protein Tyrosine Phosphatase Receptor Type C (PTPRC), CD86, transforming growth factor (TGF), CD80, CTLA4, and IL-10. CD80, ITGAM, IL-4, and PTPRC with significantly downregulated expression were identified. IL-10 and IL-2 were upregulated in the HK group but without a significant difference. Infiltration differences of CD8 lymphocyte T cells, CD4 lymphocyte T-activated memory cells, and dendritic resting cells were identified with gene CIBERSORT deconvolution algorithm analysis. Infiltration levels of CD4 lymphocyte T cell in the HK group were significantly higher than those of the K group in IHC verification. Conclusion:. HBOT affected tumor gene expression and immune cell infiltration in keloids. CD4 lymphocyte T cell, especially activated memory CD4+T, might be the key regulatory immune cell, and its related gene expression needs further study.

The compositions of the organic pollutants of chemi-mechanical pulping wastewater were studied using Fourier transform infrared spectra and gas chromatography-mass spectrometry. The chemical oxygen demand (COD) was approximately 18110 mg/L, and the ratio of biological oxygen demand to COD (BOD5/COD) was approximately 0.17, due to low biodegradability. In this study, Phanerochaete chrysosporium was selected and the changes in bacterial culture time required to transform pulping wastewater and the metabolites present before and after pretreatment in the wastewater were identified. A decline in the components of the pulping wastewater was observed. Initially, 43 kinds of organic compounds were detected in the raw water. After 3 days of treatment, only 21 organic compounds were detected. Then, 7 days later, the organic pollutants in the waste amounted to only 7 organic compounds. Through the analysis of analysis of P-RC APMP pulping wastewater and that treated by Phanerochaete chrysosporium using GCMS, the relative content of organic acids was 12.4%, in which phenylpropionic acid had the highest concentration of 11.3%. So, in the culture medium, phenylpropionic acid as the single carbon source can be completely degraded within 96 h. [ABSTRACT FROM AUTHOR]