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1. A substrate feeding strategy--using an oxystat for L-phenylalanine fermentation by Corynebacterium glutamicum

Author

Chin Hang Shu, Pei Ming Wang, Chuen Yang Yeh, and Chii Cherng Liao

Subjects
Chromatography, biology, Chemistry, Substrate (chemistry), Concentration effect, Phenylalanine, biology.organism_classification, Applied Microbiology and Biotechnology, Biochemistry, Corynebacterium glutamicum, Fermentation, Low dissolved oxygen, Control methods, Bacteria
Abstract

A substrate feeding strategy using an oxystat was first successfully applied to a fed-batch phenylalanine fermentation. The control method allowed the fermentation to be under low dissolved oxygen tension, which was favourable phenylalanine formation, and to be from substrate inhibition during the course of fed-batch operation. The final product concentration was 3 times higher than in a batch culture.

Published
1994

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