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21 results on '"Chua, Alvin Wen Choong"'

6. Intraperitoneally Delivered Umbilical Cord Lining Mesenchymal Stromal Cells Improve Survival and Kidney Function in Murine Lupus via Myeloid Pathway Targeting

Author

Chua, Alvin Wen Choong, primary, Guo, Dianyang, additional, Tan, Jia Chi, additional, Lim, Frances Ting Wei, additional, Ong, Chee Tian, additional, Masilamani, Jeyakumar, additional, Lim, Tony Kiat Hon, additional, Hwang, William Ying Khee, additional, Lim, Ivor Jiun, additional, Chen, Jinmiao, additional, Phan, Toan Thang, additional, and Fan, Xiubo, additional

Published
2022
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10. Intraperitoneally Delivered Umbilical Cord Lining Mesenchymal Stromal Cells Improve Survival and Kidney Function in Murine Lupus via Myeloid Pathway Targeting.

Author

Chua, Alvin Wen Choong, Guo, Dianyang, Tan, Jia Chi, Lim, Frances Ting Wei, Ong, Chee Tian, Masilamani, Jeyakumar, Lim, Tony Kiat Hon, Hwang, William Ying Khee, Lim, Ivor Jiun, Chen, Jinmiao, Phan, Toan Thang, and Fan, Xiubo

Subjects
*KIDNEY physiology, *STROMAL cells, *COMPLEMENT (Immunology), *CELL survival, *NEUTROPHILS, *MESENCHYMAL stem cells, *MYELOID cells, *UMBILICAL cord
Abstract

To determine the therapeutic efficacy of human umbilical cord lining mesenchymal stromal cells (CL-MSCs) (US Patent number 9,737,568) in lupus-prone MRL/lpr (Faslpr) mice and elucidate its working mechanisms. A total of 4 doses of (20–25) × 106 cells/kg of CL-MSCs was given to 16-week-old female Faslpr mice by intraperitoneal injection. Three subsequent doses were given on 17 weeks, 18 weeks, and 22 weeks, respectively. Six-week-old Faslpr mice were used as disease pre-onset controls. Mice were monitored for 10 weeks. Mouse kidney function was evaluated by examining complement component 3 (C3) deposition, urinary albumin-to-creatinine ratio (ACR), and lupus nephritis (LN) activity and chronicity. Working mechanisms were elucidated by flow cytometry, Luminex/ELISA (detection of anti-dsDNA and isotype antibodies), and RNA sequencing. CL-MSCs improved mice survival and kidney function by reducing LN activity and chronicity and lymphocyte infiltration over 10 weeks. CL-MSCs also reduced urinary ACR, renal complement C3 deposition, anti-dsDNA, and isotype antibodies that include IgA, IgG1, IgG2a, IgG2b, and IgM. Immune and cytokine profiling demonstrated that CL-MSCs dampened inflammation by suppressing splenic neutrophils and monocytes/macrophages, reducing plasma IL-6, IL-12, and CXCL1 and stabilizing plasma interferon-γ and TNF-α. RNA sequencing further showed that CL-MSCs mediated immunomodulation via concerted action of pro-proinflammatory cytokine-induced chemokines and production of nitric oxide in macrophages. CL-MSCs may provide a novel myeloid (neutrophils and monocytes/macrophages)-targeting therapy for SLE. [ABSTRACT FROM AUTHOR]

Published
2023
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11. Keratin-Alginate Sponges Support Healing of Partial-Thickness Burns

Author

Moay, Zi Kuang, primary, Nguyen, Luong T. H., additional, Hartrianti, Pietradewi, additional, Lunny, Declan P., additional, Leavesley, David, additional, Kok, Yee Onn, additional, Chong, Si Jack, additional, Chua, Alvin Wen Choong, additional, Tee, Shang-Ian, additional, and Ng, Kee Woei, additional

Published
2021
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13. Wound healing properties of magnesium mineralized antimicrobial nanofibre dressings containing chondroitin sulphate-a comparison between blend and core-shell nanofibres

Author

Leung, Chak Ming, Dhand, Chetna, Mayandi, Venkatesh, Ramalingam, Raghavendra, Lim, Fui Ping, Barathi, Veluchamy Amutha, Dwivedi, Neeraj, Orive, Gorka, Beuerman, Roger W., Ramakrishna, Seeram, Toh, Yi Chin, Loh, Xian Jun, Verma, Navin Kumar, Chua, Alvin Wen Choong, Lakshminarayanan, Rajamani, Leung, Chak Ming, Dhand, Chetna, Mayandi, Venkatesh, Ramalingam, Raghavendra, Lim, Fui Ping, Barathi, Veluchamy Amutha, Dwivedi, Neeraj, Orive, Gorka, Beuerman, Roger W., Ramakrishna, Seeram, Toh, Yi Chin, Loh, Xian Jun, Verma, Navin Kumar, Chua, Alvin Wen Choong, and Lakshminarayanan, Rajamani

Abstract

The development of antimicrobial nanofibre dressings that can protect the injured tissues from commensal pathogens while promoting tissue regeneration finds enormous potential in plastic and reconstructive surgery practices. To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium. To extend the durability of dressings, we prepared composite dressings containing polycaprolactone (PCL) and gelatin as blend or core-shell nanofibres. Nanofibre blends presented greater tensile strength and stretchability, while core-shell nanofibres displayed superior photoluminescent properties. In a porcine model of cutaneous burn injury, both the blend and core-shell nanofibre dressings displayed improved re-epithelialization, wound closure and clinical outcome in comparison to untreated burns. Histology of the biopsied tissues indicated smooth regeneration and collagen organization of the burns treated with core-shell nanostructures than untreated burns. This study compared the physico-chemical and biological properties of composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties, highlighting their potential as wound dressings and skin substitutes.

Published
2020

15. Wound healing properties of magnesium mineralized antimicrobial nanofibre dressings containing chondroitin sulphate – a comparison between blend and core–shell nanofibres

Author

Leung, Chak Ming, primary, Dhand, Chetna, additional, Mayandi, Venkatesh, additional, Ramalingam, Raghavendra, additional, Lim, Fui Ping, additional, Barathi, Veluchamy Amutha, additional, Dwivedi, Neeraj, additional, Orive, Gorka, additional, Beuerman, Roger W., additional, Ramakrishna, Seeram, additional, Toh, Yi-Chin, additional, Loh, Xian Jun, additional, Verma, Navin Kumar, additional, Chua, Alvin Wen Choong, additional, and Lakshminarayanan, Rajamani, additional

Published
2020
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16. A Review of Skin Banking Guidelines and Standards Worldwide: Towards the Harmonization of Guidelines for Skin Banking in Therapeutic Applications for the Regions under the Asia Pacific Burn Association (APBA)

Author

Heng, Wee Ling, primary, Wang, Qi Wei, additional, Sornarajah, Renuka, additional, Tremblay, Jacynthe, additional, Putri, Nandita Melati, additional, Hamid, Suzina Sheikh Ab, additional, Pungrasmi, Pornthep, additional, Wang, Hsian-Jenn, additional, Kim, Dong Chul, additional, Saito, Daizo, additional, Nguyen, Nhu Lam, additional, Sulaiman, Wan Azman Wan, additional, Wardhana, Aditya, additional, Puri, Vinita, additional, Matsumura, Hajime, additional, Dai, Niann-Tzyy, additional, Ahuja, Rajeev B, additional, Luo, Gaoxing, additional, He, Weifeng, additional, Chong, Si Jack, additional, and Chua, Alvin Wen Choong, additional

Published
2020
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17. Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins

Author

Tjin, Monica Suryana, Chua, Alvin Wen Choong, Ma, Dong Rui, Lee, Seng Teik, Fong, Eileen, and School of Materials Science & Engineering

Subjects
Science::Biological sciences [DRNTU]
Abstract

Cell-matrix interactions play critical roles in regulating cellular behavior in wound repair and regeneration of the human skin. In particular, human skin keratinocytes express several key integrins such as alpha5beta1, alpha3beta1, and alpha2beta1 for binding to the extracellular matrix (ECM) present in the basement membrane in uninjured skin. To mimic these key integrin-ECM interactions, artificial ECM (aECM) proteins containing functional domains derived from laminin 5, type IV collagen, fibronectin, and elastin are prepared. Human skin keratinocyte cell responses on the aECM proteins are specific to the cell-binding domain present in each construct. Keratinocyte attachment to the aECM protein substrates is also mediated by specific integrin-material interactions. In addition, the aECM proteins are able to support the proliferation of keratinocyte stem cells, demonstrating their promise for use in skin tissue engineering.

Published
2014

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