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With global nitrogen (N) deposition continuously increasing, few reports exist describing how soil bacteria respond at the molecular level to long-term addition of excess N in variously aged forests. To reveal the responses of soil bacteria to the elevated N, an experiment was conducted with a chronic nutrient addition within differently aged stands (46-, 78-, and 200-years-old) in the northern subtropical China since 2011, including three treatments, CK (no N nor phosphorus (P) additions), N treatment (N, 100 kg N ha-1), and N with P (N+P, 100 kg N ha-1 + 50 kg P ha-1) to examine potential P limitation under N deposition. Metagenomic sequencing was used to examine the snapshot responses of soil bacterial communities. Soil moisture and texture, ammonium, nitrate, SOC (soil organic carbon), TN (soil total nitrogen), TP (total phosphorus), DOC (dissolved organic carbon), DON (dissolved organic nitrogen) were measured to explain the influence mechanism of forest age and fertilization on changes of microbial community. Following N addition, soil bacterial community diversity and most dominant phyla increased, but they showed a decrease with increasing stand age. The effects of fertilization on the same taxa were variable across forest ages. Soil bacterial community responded differently in 7-year fertilization, with distinct shift in 46-year-old forest and adaptability to long-term N addition in the 200-year-old forest. Soil texture and moisture, DOC, DON, pH, SOC/TN and TP were significantly correlated with bacterial community across stand ages, while N fertilization affected the bacterial community mostly via inducing soil moisture, NO3--N, DOC and pH in the 46-year-old forest, whose effects decreased with increasing stand age. Our results suggest that due to the variation of soil physicochemical properties among forest ages, soil bacterial communities are more stable and resilient to N deposition with increasing the age of stands. Soil bacterial communities might not encounter P limitation following the long-term addition of N in the subtropics.

Despite the prevalence and significant morbidity resulting from estrogen receptor positive (ER+) breast adenocarcinomas, there are only a few models of this cancer subtype available for drug development and arguably none for studying etiology. Those models that do exist have questionable clinical relevance. Given our goal of developing luminal models, we focused on six cell lines derived by minimal mutagenesis from normal human breast cells, and asked if any could generate clinically relevant xenografts, which we then extensively characterized. Xenografts of one cell line, 184AA3, consistently formed ER+ adenocarcinomas that had a high proliferative rate and other features consistent with “luminal B” intrinsic subtype. Squamous and spindle cell/mesenchymal differentiation was absent, in stark contrast to other cell lines that we examined or others have reported. We explored intratumoral heterogeneity produced by 184AA3 by immunophenotyping xenograft tumors and cultured cells, and characterized marker expression by immunofluorescence and flow cytometry. A CD44High subpopulation was discovered, yet their tumor forming ability was far less than CD44Low cells. Single cell cloning revealed the phenotypic plasticity of 184AA3, consistent with the intratumoral heterogeneity observed in xenografts. Characterization of ER expression in cultures revealed ER protein and signaling is intact, yet when estrogen was depleted in culture, and in vivo, it did not impact cell or tumor growth, analogous to therapeutically resistant ER+ cancers. This model is appropriate for studies of the etiology of ovarian hormone independent adenocarcinomas, for identification of therapeutic targets, predictive testing, and drug development.

The 1999 Chi-Chi, Taiwan, earthquake provides case histories of ground failure and non-ground failure that are valuable to the ongoing development of liquefaction susceptibility, triggering and surface manifestation models because the data occupy sparsely populated parameter spaces (i.e. high cyclic stress ratio and high fines content with low to moderate soil plasticity). In this paper, we document results from several large site investigation programs conducted in Nantou, Wufeng and Yuanlin, Taiwan. The seismic performance of the investigated sites include non-ground failure building and free-field sites, building sites with partial foundation hearing failures, free-field lateral spread sites, and free-field level ground sites with sediment boils. Field and laboratory investigation protocols for the sites are described, including cone penetration testing (some with pore pressure and shear wave velocity measurements) and rotary wash borings with standard penetration testing (including energy measurements). Implications of the SPT energy measurements with respect to established guidelines for the estimation of SPT energy ratio (including short rod corrections) are presented. Finally, data for three example sites are shown that illustrate potential applications of the data set, and which also demonstrate a condition where existing liquefaction analysis procedures fail to predict the observed field performance. (C) 2004 Elsevier Ltd. All rights reserved.

E-commerce has been adopted by businesses and consumers in Vietnam for consumption activities, profit-seeking, buying and selling goods, conducting business, competing, and expanding markets. Businesses have chosen e-commerce due to its benefits, the trend of applying digital technology, and the ongoing modern business environment. Many studies have identified factors influencing the acceptance of e-commerce, but only some studies have approached sustainable e-commerce. Developing sustainable e-commerce requires businesses to invest more money and technology in business processes, which consumers may only sometimes accept. This article applies the TOE framework to identify factors influencing the acceptance of sustainable e-commerce by measuring the importance coefficient of these factors. The research results reflect changes in the factors affecting the acceptance of sustainable e-commerce. The study also provides recommendations for stakeholders involved in developing sustainable e-commerce.