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Remote sensing satellite images can intuitively reflect the information of the Earth's surface. The computer image processing system is of the advantages of high-precision and low-cost. It has a strong application value to study the computer processing system of remote sensing satellite image. The paper first discussed the design principles of the computer processing system and the implementation of its workflow, and then the application of the image processing system is briefly analyzed.

Our previous studies have demonstrated the cystic fibrosis transmembrane conductance regulator (CFTR) is important for capacitation and male fertility in mouse and guinea pig spermatozoa. However, the exact function of CFTR on human sperm fertilizing capacity, and correlation with sperm quality has not been established. The present study may shed light on some unexplained male infertility, and on a possible new method for diagnosis of male infertility and strategy for male contraception.To assess the effect of CFTR on human sperm fertilizing capacity, we examined sperm capacitation and the acrosome reaction using chlortetracycline staining, analyzed sperm hyperactivation by computer-assisted semen analysis (CASA), measured intracellular cAMP levels using ElA and evaluated sperm penetration of zona-free hamster eggs assay in fertile men. The percentage of spermatozoa expressing CFTR from fertile, healthy and infertile men (mainly teratospermic, asthenoteratospermic, asthenospermic and oligospermic) was conducted by indirect immunofluorescence staining.Progesterone significantly facilitated human sperm capacitation and ZP3 triggered the acrosome reaction, both were significantly inhibited by CFTR inhibitor-172 (CFTRinh-172; 10 nM-1 microM) in a dose-dependent manner. The presence of 100 nM CFTRinh-172 markedly depressed intracellular cAMP levels, sperm hyperactivation and sperm penetration of zona-free hamster eggs. In addition, the percentage of spermatozoa expressing CFTR in the fertile men was significantly higher than healthy and infertile men categories (P0.01).CFTR is essential for human sperm fertilizing capacity and the impairment of CFTR expression in spermatozoa is correlated with a reduction of sperm quality. These results suggest that defective expression of CFTR in human sperm may lead to the reduction of sperm fertilizing capacity.

To study the expression of protein kinase C (PKC) alpha subtype in Chinese hamster ovary (CHO) cells.The PKC alpha primer pairs were designed based on the GenBank sequence of PKC alpha of a species with the highest homology to Chinese hamster identified using EMBL Data Library Clustalw tool. The sequence coding for PKC alpha, amplified from the CHO cells using RT-PCR, was ligated to the pGEM-T plasmid vector, and the recombinant vector was transformed into E.coli DH5alpha with the positive colones selected by blue/white screening. Restriction enzyme digestion, gel electrophoresis analysis, followed by sequencing of the digestion products were performed for identification of the recombinant. Western blotting was used to analyze the PKC alpha expression in the CHO cells.The presence of PKC alpha mRNA was detected in the CHO cells by RT-PCR. Western blotting also identified PKC alpha expression in the cells.PKC alpha expression has been identified in the CHO cells, which may facilitate further structural and functional study of PKC alpha and investigation of its role in the intracellular signal transduction pathways.

This paper introduces a new physical parameter — thermodynamic shear advection parameter combining the perturbation vertical component of convective vorticity vector with the coupling of horizontal divergence perturbation and vertical gradient of general potential temperature perturbation. For a heavy-rainfall event resulting from the landfall typhoon 'Wipha', the parameter is calculated by using National Centres for Enviromental Prediction/National Centre for Atmospheric Research global final analysis data. The results showed that the parameter corresponds to the observed 6 h accumulative rainband since it is capable of catching hold of the dynamic and thermodynamic disturbance in the lower troposphere over the observed rainband. Before the typhoon landed, the advection of the parameter by basic-state flow and the coupling of general potential temperature perturbation with curl of Coriolis force perturbation are the primary dynamic processes which are responsible for the local change of the parameter. After the typhoon landed, the disturbance is mainly driven by the combination of five primary dynamic processes. The advection of the parameter by basic-state flow was weakened after the typhoon landed.

On the basis of vertical helicity, we define three parameters, namely, the divergence flux, moisture vertical helicity and moisture divergence flux. The parameters are calculated with NCEP/NCAR real analysis data in the two heavy-rainfall events occurring in China. The result shows that the parameters are closely related to the precipitation systems since they are capable of describing the typical vertical structure of dynamical and moisture fields. The anomalies of the parameters vertically integrated well correspond with the observed 6h-accumulated surface rainfall. This suggests that the parameters are capable of predicting and indicating the development and propagation of precipitation systems. The calculation with the 6-hour forecasting data of NCEP-GFS shows that the parameters have the ability of predicting precipitation and the moisture divergence flux is significantly beller than the other parameters in predicting precipitation.