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17 results on '"Chu, Pinpin"'

2. RPA-CRISPR/Cas12a-Based Detection of Haemophilus parasuis

Author

Zhang, Kunli, primary, Sun, Zeyi, additional, Shi, Keda, additional, Yang, Dongxia, additional, Bian, Zhibiao, additional, Li, Yan, additional, Gou, Hongchao, additional, Jiang, Zhiyong, additional, Yang, Nanling, additional, Chu, Pinpin, additional, Zhai, Shaolun, additional, Wei, Zhanyong, additional, and Li, Chunling, additional

Published
2023
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3. Accelerated loop-mediated isothermal amplification method for the rapid detection of Streptococcus suis serotypes 2 and 14 based on single nucleotide polymorphisms

Author

Meng, Jiajia, primary, Li, Chunling, additional, Wang, Yu, additional, Bian, Zhibiao, additional, Chu, Pinpin, additional, Zhai, Shaolun, additional, Yang, Dongxia, additional, Song, Shuai, additional, Li, Yan, additional, Jiang, Zhiyong, additional, Zhang, Kunli, additional, Li, Yugu, additional, and Gou, Hongchao, additional

Published
2022
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6. YBX has functional roles in CpG-ODN against cold stress and bacterial infection of Misgurnus anguillicaudatus

Author

Zhao, Zengjue, primary, He, Rongxiao, additional, Chu, Pinpin, additional, Cai, Haiming, additional, Shen, Haokun, additional, Zhao, Zitong, additional, Feng, Saixiang, additional, Cao, Ding, additional, Liao, Ming, additional, Gan, Guanhua, additional, Ye, Hejia, additional, Chen, Zhiyang, additional, Qiu, Weihong, additional, Deng, Jinbo, additional, Ming, Feiping, additional, Ma, Miaopeng, additional, Jia, Junhao, additional, Wu, Jiahui, additional, Huang, Haobin, additional, Sun, Chongjun, additional, Li, Jiayi, additional, and Zhang, Linghua, additional

Published
2021
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9. Intestinal guard: Human CXCL17 modulates protective response against mycotoxins and CXCL17-mimetic peptides development

Author

Sun, Chongjun, primary, Shen, Haokun, additional, Cai, Haiming, additional, Zhao, Zengjue, additional, Gan, Guanhua, additional, Feng, Saixiang, additional, Chu, Pinpin, additional, Zeng, Min, additional, Deng, Jinbo, additional, Ming, Feiping, additional, Ma, Miaopeng, additional, Jia, Junhao, additional, He, Rongxiao, additional, Cao, Ding, additional, Chen, Zhiyang, additional, Li, Jiayi, additional, and Zhang, Linghua, additional

Published
2021
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10. Rapid detection of Haemophilus parasuis using cross-priming amplification and vertical flow visualization

Author

Li Chunling, Li Miao, Gou Hongchao, Cai Rujian, Li Yan, Chu Pinpin, Song Shuai, Jiang Zhiyong, Yang Dongxia, and Juan Li

Subjects
DNA, Bacterial, 0301 basic medicine, Microbiology (medical), Serotype, Haemophilus Infections, Swine, Point-of-care testing, 030106 microbiology, Cross-priming, Polymerase Chain Reaction, Sensitivity and Specificity, Microbiology, Rapid detection, law.invention, Haemophilus parasuis, 03 medical and health sciences, High morbidity, Cross-Priming, Species Specificity, law, Haemophilus, Vertical flow, Animals, Molecular Biology, Polymerase chain reaction, DNA Primers, Swine Diseases, biology, biology.organism_classification, Virology, Bacterial Typing Techniques, 030104 developmental biology, Molecular Diagnostic Techniques
Abstract

Haemophilus parasuis infection is of considerable economic importance in the swine industry due to high morbidity and mortality in naive swine populations. Accurate detection and identification of the causative agent are difficult, yet necessary, for disease control. In this study, a simple and rapid method of cross-priming amplification (CPA) with a vertical flow (VF) visualization strip was established to detect H. parasuis. The reaction can specifically identify 15 serovar reference strains and 57 clinically isolated strains of H. parasuis, with a detection limit of 14CFU. The performance of the CPA-VF assay was evaluated and compared with that of species-specific PCR by testing 62 clinical culture-positive specimens of H. parasuis. The entire process, from specimen processing to analysis of the results, can be completed in 2h without a complicated apparatus. The convenience and speed of the CPA-VF assay in this study make it a suitable choice for epidemiological investigation and point-of-care testing (POCT) for H. parasuis infection.

Published
2018

14. Evaluation of immunogenicity and protective efficacy of recombinant outer membrane proteins of Haemophilus parasuis serovar 5 in a murine model

Author

Chu Pinpin, Hua-Ji Qiu, Cai Rujian, Jiang Zhiyong, Li Miao, Song Shuai, Li Yan, Gou Hongchao, and Li Chunling

Subjects
0301 basic medicine, Serotype, Physiology, Cell Membranes, lcsh:Medicine, Biochemistry, law.invention, Haemophilus parasuis, Mice, law, Immune Physiology, Medicine and Health Sciences, Public and Occupational Health, Enzyme-Linked Immunoassays, lcsh:Science, Immune Response, Vaccines, Multidisciplinary, Immune System Proteins, biology, Immunogenicity, Vaccination, Vaccination and Immunization, Antibodies, Bacterial, Recombinant Proteins, Infectious Diseases, Recombinant DNA, Antibody, Cellular Structures and Organelles, Research Article, Antigenicity, Infectious Disease Control, 030106 microbiology, Immunology, Research and Analysis Methods, Antibodies, 03 medical and health sciences, Immune system, Antigen, Bacterial Proteins, Vaccine Development, Animals, Antigens, Immunoassays, lcsh:R, Biology and Life Sciences, Proteins, Membrane Proteins, Cell Biology, Outer Membrane Proteins, Virology, 030104 developmental biology, biology.protein, Immunologic Techniques, lcsh:Q, Preventive Medicine, Lymphoproliferative response
Abstract

Glasser's disease is an economically important infectious disease of pigs caused by Haemophilus parasuis. Few vaccines are currently available that could provide effective cross-protection against various serovars of H. parasuis. In this study, five OMPs (OppA, TolC, HxuC, LppC, and HAPS_0926) identified by bioinformatic approaches, were cloned and expressed as recombinant proteins. Antigenicity of the purified proteins was verified through Western blotting, and primary screening for protective potential was evaluated in vivo. Recombinant TolC (rTolC), rLppC, and rHAPS_0926 proteins showing marked protection of mice against H. parasuis infection, and were further evaluated individually or in combination. Mice treated with these three OMPs produced humoral and host cell-mediated responses, with a significant rise in antigen-specific IgG titer and lymphoproliferative response in contrast with the mock-immunized group. Significant increases were noted in CD4+, CD8+ T cells, and three cytokines (IL-2, IL-4, and IFN-γ) in vaccinated animals. The antisera against candidate antigens could efficiently impede bacterial survival in whole blood bactericidal assay against H. parasuis infection. The multi-protein vaccine induced more pronounced immune responses and offered better protection than individual vaccines. Our findings indicate that these three OMPs are promising antigens for the development of multi-component subunit vaccines against Glasser's disease.

Published
2017

16. Immunization of Aged Pigs with Attenuated Pseudorabies Virus Vaccine Combined with CpG Oligodeoxynucleotide Restores Defective Th1 Immune Responses

Author

Ming, Feiping, primary, Yang, Jun, additional, Chu, Pinpin, additional, Ma, Miaopeng, additional, Shi, Juqing, additional, Cai, Haiming, additional, Huang, Chaoyuan, additional, Li, Huazhou, additional, Jiang, Zhenggu, additional, Wang, Houguang, additional, Wang, Weifang, additional, Zhang, Shuiqing, additional, and Zhang, Linghua, additional

Published
2013
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17. Outer membrane vesicles secreted from Actinobacillus pleuropneumoniae isolate disseminating the floR resistance gene to Enterobacteriaceae.

Author

Xu M, Ke H, Zang Y, Gou H, Yang D, Shi K, Zhang K, Li Y, Jiang Z, Chu P, Zhai S, and Li C

Abstract

Actinobacillus pleuropneumoniae , a significant respiratory pig pathogen, is causing substantial losses in the global swine industry. The resistance spectrum of A. pleuropneumoniae is expanding, and multidrug resistance is a severe issue. Horizontal gene transfer (HGT) plays a crucial role in the development of the bacterial genome by facilitating the dissemination of resistance determinants. However, the horizontal transfer of resistance genes via A. pleuropneumoniae -derived outer membrane vesicles (OMVs) has not been previously reported. In this study, we used Illumina NovaSeq and PacBio SequeI sequencing platforms to determine the whole genome sequence of A. pleuropneumoniae GD2107, a multidrug-resistant (MDR) isolate from China. We detected a plasmid in the isolate named pGD2107-1; the plasmid was 5,027 bp in size with 7 putative open reading frames (ORF) and included the floR resistance genes. The carriage of resistance genes in A. pleuropneumoniae OMVs was identified using a polymerase chain reaction (PCR) assay, and then we thoroughly evaluated the influence of OMVs on the horizontal transfer of drug-resistant plasmids. The transfer of the plasmid to recipient bacteria via OMVs was confirmed by PCR. In growth competition experiments, all recipients carrying the pGD2107-1 plasmid exhibited a fitness cost compared to the corresponding original recipients. This study revealed that OMVs could mediate interspecific horizontal transfer of the resistance plasmid pGD2107-1 into Escherichia coli recipient strains and significantly enhance the resistance of the transformants. In summary, A. pleuropneumoniae -OMVs play the pivotal role of vectors for dissemination of the floR gene spread and may contribute to more antimicrobial resistance gene transfer in other Enterobacteriaceae., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Xu, Ke, Zang, Gou, Yang, Shi, Zhang, Li, Jiang, Chu, Zhai and Li.)

Published
2024
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