177,562 results on '"Chromosomes"'
Search Results
2. Integration of CTCF loops, methylome, and transcriptome in differentiating LUHMES as a model for imprinting dynamics of the 15q11-q13 locus in human neurons
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Fugón, Orangel J Gutierrez, Sharifi, Osman, Heath, Nicholas, Soto, Daniela C, Gomez, J Antonio, Yasui, Dag H, Mendiola, Aron Judd P, O’Geen, Henriette, Beitnere, Ulrika, Tomkova, Marketa, Haghani, Viktoria, Dillon, Greg, Segal, David J, and LaSalle, Janine M
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Biological Sciences ,Genetics ,Neurosciences ,Human Genome ,Brain Disorders ,Intellectual and Developmental Disabilities (IDD) ,Pediatric ,Rare Diseases ,Stem Cell Research ,Stem Cell Research - Induced Pluripotent Stem Cell ,1.1 Normal biological development and functioning ,Generic health relevance ,chromatin ,imprinting ,human cell models ,Angelman ,Prader-Willi Syndrome ,LUHMES ,methylation ,UBE3A ,Humans ,Genomic Imprinting ,CCCTC-Binding Factor ,Chromosomes ,Human ,Pair 15 ,Neurons ,DNA Methylation ,Transcriptome ,Ubiquitin-Protein Ligases ,Cell Differentiation ,Angelman Syndrome ,RNA ,Long Noncoding ,snRNP Core Proteins ,Alleles ,Cell Line ,Epigenome ,Medical and Health Sciences ,Genetics & Heredity - Abstract
Human cell line models, including the neuronal precursor line LUHMES, are important for investigating developmental transcriptional dynamics within imprinted regions, particularly the 15q11-q13 Angelman (AS) and Prader-Willi (PWS) syndrome locus. AS results from loss of maternal UBE3A in neurons, where the paternal allele is silenced by a convergent antisense transcript UBE3A-ATS, a lncRNA that terminates at PWAR1 in non-neurons. qRT-PCR analysis confirmed the exclusive and progressive increase in UBE3A-ATS in differentiating LUHMES neurons, validating their use for studying UBE3A silencing. Genome-wide transcriptome analyses revealed changes to 11 834 genes during neuronal differentiation, including the upregulation of most genes within the 15q11-q13 locus. To identify dynamic changes in chromatin loops linked to transcriptional activity, we performed a HiChIP validated by 4C, which identified two neuron-specific CTCF loops between MAGEL2-SNRPN and PWAR1-UBE3A. To determine if allele-specific differentially methylated regions (DMR) may be associated with CTCF loop anchors, whole genome long-read nanopore sequencing was performed. We identified a paternally hypomethylated DMR near the SNRPN upstream loop anchor exclusive to neurons and a paternally hypermethylated DMR near the PWAR1 CTCF anchor exclusive to undifferentiated cells, consistent with increases in neuronal transcription. Additionally, DMRs near CTCF loop anchors were observed in both cell types, indicative of allele-specific differences in chromatin loops regulating imprinted transcription. These results provide an integrated view of the 15q11-q13 epigenetic landscape during LUHMES neuronal differentiation, underscoring the complex interplay of transcription, chromatin looping, and DNA methylation. They also provide insights for future therapeutic approaches for AS and PWS.
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- 2024
3. Nitrogen deficiency tolerance conferred by introgression of a QTL derived from wild emmer into bread wheat.
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Govta, Nikolai, Fatiukha, Andrii, Govta, Liubov, Pozniak, Curtis, Distelfeld, Assaf, Fahima, Tzion, Beckles, Diane, and Krugman, Tamar
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Triticum ,Quantitative Trait Loci ,Nitrogen ,Phenotype ,Genetic Introgression ,Chromosome Mapping ,Stress ,Physiological ,Droughts ,Chromosomes ,Plant - Abstract
Genetic dissection of a QTL from wild emmer wheat, QGpc.huj.uh-5B.2, introgressed into bread wheat, identified candidate genes associated with tolerance to nitrogen deficiency, and potentially useful for improving nitrogen-use efficiency. Nitrogen (N) is an important macronutrient critical to wheat growth and development; its deficiency is one of the main factors causing reductions in grain yield and quality. N availability is significantly affected by drought or flooding, that are dependent on additional factors including soil type or duration and severity of stress. In a previous study, we identified a high grain protein content QTL (QGpc.huj.uh-5B.2) derived from the 5B chromosome of wild emmer wheat, that showed a higher proportion of explained variation under water-stress conditions. We hypothesized that this QTL is associated with tolerance to N deficiency as a possible mechanism underlying the higher effect under stress. To validate this hypothesis, we introgressed the QTL into the elite bread wheat var. Ruta, and showed that under N-deficient field conditions the introgression IL99 had a 33% increase in GPC (p
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- 2024
4. Transcriptional immune suppression and up-regulation of double-stranded DNA damage and repair repertoires in ecDNA-containing tumors.
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Lin, Miin, Jo, Se-Young, Luebeck, Jens, Chang, Howard, Wu, Sihan, Mischel, Paul, and Bafna, Vineet
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cancer ,chromosomes ,ecDNA ,extrachromosomal DNA ,gene expression ,human ,transcriptomics ,Humans ,DNA Repair ,DNA Damage ,Neoplasms ,Up-Regulation ,Gene Expression Regulation ,Neoplastic ,Transcription ,Genetic - Abstract
Extrachromosomal DNA is a common cause of oncogene amplification in cancer. The non-chromosomal inheritance of ecDNA enables tumors to rapidly evolve, contributing to treatment resistance and poor outcome for patients. The transcriptional context in which ecDNAs arise and progress, including chromosomally-driven transcription, is incompletely understood. We examined gene expression patterns of 870 tumors of varied histological types, to identify transcriptional correlates of ecDNA. Here, we show that ecDNA-containing tumors impact four major biological processes. Specifically, ecDNA-containing tumors up-regulate DNA damage and repair, cell cycle control, and mitotic processes, but down-regulate global immune regulation pathways. Taken together, these results suggest profound alterations in gene regulation in ecDNA-containing tumors, shedding light on molecular processes that give rise to their development and progression.
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- 2024
5. Modeling homologous chromosome recognition via nonspecific interactions
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Marshall, Wallace F and Fung, Jennifer C
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Biological Sciences ,Bioinformatics and Computational Biology ,Genetics ,Underpinning research ,1.1 Normal biological development and functioning ,Animals ,Models ,Genetic ,Chromosome Pairing ,Drosophila melanogaster ,Chromosomes ,Drosophila ,Computer Simulation ,Chromosomes ,Insect ,somatic homolog pairing ,polymer dynamics ,computational modeling ,chromosome dynamics - Abstract
In many organisms, most notably Drosophila, homologous chromosomes associate in somatic cells, a phenomenon known as somatic pairing, which takes place without double strand breaks or strand invasion, thus requiring some other mechanism for homologs to recognize each other. Several studies have suggested a "specific button" model, in which a series of distinct regions in the genome, known as buttons, can associate with each other, mediated by different proteins that bind to these different regions. Here, we use computational modeling to evaluate an alternative "button barcode" model, in which there is only one type of recognition site or adhesion button, present in many copies in the genome, each of which can associate with any of the others with equal affinity. In this model, buttons are nonuniformly distributed, such that alignment of a chromosome with its correct homolog, compared with a nonhomolog, is energetically favored; since to achieve nonhomologous alignment, chromosomes would be required to mechanically deform in order to bring their buttons into mutual register. By simulating randomly generated nonuniform button distributions, many highly effective button barcodes can be easily found, some of which achieve virtually perfect pairing fidelity. This model is consistent with existing literature on the effect of translocations of different sizes on homolog pairing. We conclude that a button barcode model can attain highly specific homolog recognition, comparable to that seen in actual cells undergoing somatic homolog pairing, without the need for specific interactions. This model may have implications for how meiotic pairing is achieved.
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- 2024
6. 5G2 mutant mice model loss of a commonly deleted segment of chromosome 7q22 in myeloid malignancies.
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Wong, Jasmine, Weinfurtner, Kelley, Westover, Tamara, Kim, Jangkyung, Lebish, Eric, Del Pilar Alzamora, Maria, Walsh, Michael, Abdelhamed, Sherif, Ma, Jing, Klco, Jeffery, Shannon, Kevin, and Huang, Benjamin
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Animals ,Mice ,Chromosome Deletion ,Disease Models ,Animal ,Chromosomes ,Human ,Pair 7 ,Humans ,Leukemia ,Myeloid ,Acute ,Myelodysplastic Syndromes ,Mice ,Inbred C57BL - Abstract
Monosomy 7 and del(7q) are among the most common and poorly understood genetic alterations in myelodysplastic neoplasms and acute myeloid leukemia. Chromosome band 7q22 is a minimally deleted segment in myeloid malignancies with a del(7q). However, the rarity of second hit mutations supports the idea that del(7q22) represents a contiguous gene syndrome. We generated mice harboring a 1.5 Mb germline deletion of chromosome band 5G2 syntenic to human 7q22 that removes Cux1 and 27 additional genes. Hematopoiesis is perturbed in 5G2+/del mice but they do not spontaneously develop hematologic disease. Whereas alkylator exposure modestly accelerated tumor development, the 5G2 deletion did not cooperate with KrasG12D, NrasG12D, or the MOL4070LTR retrovirus in leukemogenesis. 5G2+/del mice are a novel platform for interrogating the role of hemopoietic stem cell attrition/stress, cooperating mutations, genotoxins, and inflammation in myeloid malignancies characterized by monosomy 7/del(7q).
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- 2024
7. Ribosome subunit attrition and activation of the p53-MDM4 axis dominate the response of MLL-rearranged cancer cells to WDR5 WIN site inhibition.
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Howard, Gregory, Wang, Jing, Rose, Kristie, Jones, Camden, Patel, Purvi, Tsui, Tina, Florian, Andrea, Vlach, Logan, Lorey, Shelly, Grieb, Brian, Smith, Brianna, Slota, Macey, Reynolds, Elizabeth, Goswami, Soumita, Savona, Michael, Mason, Frank, Lee, Taekyu, Fesik, Stephen, Liu, Qi, and Tansey, William
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WDR5 ,cancer biology ,cancer therapy ,chromosomes ,gene expression ,human ,p53 ,ribosomes ,Humans ,Antineoplastic Agents ,Cell Cycle Proteins ,Cell Line ,Tumor ,Histone-Lysine N-Methyltransferase ,Intracellular Signaling Peptides and Proteins ,Myeloid-Lymphoid Leukemia Protein ,Nuclear Proteins ,Proto-Oncogene Proteins ,Ribosomes ,Tumor Suppressor Protein p53 ,Peptidomimetics - Abstract
The chromatin-associated protein WD Repeat Domain 5 (WDR5) is a promising target for cancer drug discovery, with most efforts blocking an arginine-binding cavity on the protein called the WIN site that tethers WDR5 to chromatin. WIN site inhibitors (WINi) are active against multiple cancer cell types in vitro, the most notable of which are those derived from MLL-rearranged (MLLr) leukemias. Peptidomimetic WINi were originally proposed to inhibit MLLr cells via dysregulation of genes connected to hematopoietic stem cell expansion. Our discovery and interrogation of small-molecule WINi, however, revealed that they act in MLLr cell lines to suppress ribosome protein gene (RPG) transcription, induce nucleolar stress, and activate p53. Because there is no precedent for an anticancer strategy that specifically targets RPG expression, we took an integrated multi-omics approach to further interrogate the mechanism of action of WINi in human MLLr cancer cells. We show that WINi induce depletion of the stock of ribosomes, accompanied by a broad yet modest translational choke and changes in alternative mRNA splicing that inactivate the p53 antagonist MDM4. We also show that WINi are synergistic with agents including venetoclax and BET-bromodomain inhibitors. Together, these studies reinforce the concept that WINi are a novel type of ribosome-directed anticancer therapy and provide a resource to support their clinical implementation in MLLr leukemias and other malignancies.
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- 2024
8. The complex polyploid genome architecture of sugarcane
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Healey, AL, Garsmeur, O, Lovell, JT, Shengquiang, S, Sreedasyam, A, Jenkins, J, Plott, CB, Piperidis, N, Pompidor, N, Llaca, V, Metcalfe, CJ, Doležel, J, Cápal, P, Carlson, JW, Hoarau, JY, Hervouet, C, Zini, C, Dievart, A, Lipzen, A, Williams, M, Boston, LB, Webber, J, Keymanesh, K, Tejomurthula, S, Rajasekar, S, Suchecki, R, Furtado, A, May, G, Parakkal, P, Simmons, BA, Barry, K, Henry, RJ, Grimwood, J, Aitken, KS, Schmutz, J, and D’Hont, A
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Agricultural ,Veterinary and Food Sciences ,Biological Sciences ,Genetics ,Agricultural Biotechnology ,Crop and Pasture Production ,Horticultural Production ,Human Genome ,Biotechnology ,Zero Hunger ,Chromosomes ,Plant ,Genome ,Plant ,Haplotypes ,Hybridization ,Genetic ,Plant Breeding ,Polyploidy ,Saccharum ,Reference Standards ,DNA ,Plant ,General Science & Technology - Abstract
Sugarcane, the world's most harvested crop by tonnage, has shaped global history, trade and geopolitics, and is currently responsible for 80% of sugar production worldwide1. While traditional sugarcane breeding methods have effectively generated cultivars adapted to new environments and pathogens, sugar yield improvements have recently plateaued2. The cessation of yield gains may be due to limited genetic diversity within breeding populations, long breeding cycles and the complexity of its genome, the latter preventing breeders from taking advantage of the recent explosion of whole-genome sequencing that has benefited many other crops. Thus, modern sugarcane hybrids are the last remaining major crop without a reference-quality genome. Here we take a major step towards advancing sugarcane biotechnology by generating a polyploid reference genome for R570, a typical modern cultivar derived from interspecific hybridization between the domesticated species (Saccharum officinarum) and the wild species (Saccharum spontaneum). In contrast to the existing single haplotype ('monoploid') representation of R570, our 8.7 billion base assembly contains a complete representation of unique DNA sequences across the approximately 12 chromosome copies in this polyploid genome. Using this highly contiguous genome assembly, we filled a previously unsized gap within an R570 physical genetic map to describe the likely causal genes underlying the single-copy Bru1 brown rust resistance locus. This polyploid genome assembly with fine-grain descriptions of genome architecture and molecular targets for biotechnology will help accelerate molecular and transgenic breeding and adaptation of sugarcane to future environmental conditions.
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- 2024
9. Chromosome-level assembly of the gray fox (Urocyon cinereoargenteus) confirms the basal loss of PRDM9 in Canidae.
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Armstrong, Ellie, Bissell, Ky, Fatima, H, Heikkinen, Maya, Jessup, Anika, Junaid, Maryam, Lee, Dong, Lieb, Emily, Liem, Josef, Martin, Estelle, Moreno, Mauricio, Otgonbayar, Khuslen, Romans, Betsy, Royar, Kim, Adler, Mary, Needle, David, Harkess, Alex, Kelley, Joanna, Mooney, Jazlyn, and Mychajliw, Alexis
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Canidae ,PRDM9 ,gray fox ,heterozygosity ,Animals ,Foxes ,Phylogeny ,Chromosomes ,DNA ,Mitochondrial ,Genome - Abstract
Reference genome assemblies have been created from multiple lineages within the Canidae family; however, despite its phylogenetic relevance as a basal genus within the clade, there is currently no reference genome for the gray fox (Urocyon cinereoargenteus). Here, we present a chromosome-level assembly for the gray fox (U. cinereoargenteus), which represents the most contiguous, non-domestic canid reference genome available to date, with 90% of the genome contained in just 34 scaffolds and a contig N50 and scaffold N50 of 59.4 and 72.9 Megabases, respectively. Repeat analyses identified an increased number of simple repeats relative to other canids. Based on mitochondrial DNA, our Vermont sample clusters with other gray fox samples from the northeastern United States and contains slightly lower levels of heterozygosity than gray foxes on the west coast of California. This new assembly lays the groundwork for future studies to describe past and present population dynamics, including the delineation of evolutionarily significant units of management relevance. Importantly, the phylogenetic position of Urocyon allows us to verify the loss of PRDM9 functionality in the basal canid lineage, confirming that pseudogenization occurred at least 10 million years ago.
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- 2024
10. Single-cell multiplex chromatin and RNA interactions in ageing human brain.
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Wen, Xingzhao, Luo, Zhifei, Zhao, Wenxin, Calandrelli, Riccardo, Nguyen, Tri, Wan, Xueyi, Charles Richard, John, and Zhong, Sheng
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Aged ,Female ,Humans ,Male ,Aging ,Alzheimer Disease ,Cell Nucleus ,Cellular Senescence ,Chromatin ,Chromosomes ,Human ,X ,Frontal Lobe ,Gene Expression Profiling ,Promoter Regions ,Genetic ,Quantitative Trait Loci ,RNA ,RNA ,Long Noncoding ,Single-Cell Analysis ,Transcription ,Genetic - Abstract
Dynamically organized chromatin complexes often involve multiplex chromatin interactions and sometimes chromatin-associated RNA1-3. Chromatin complex compositions change during cellular differentiation and ageing, and are expected to be highly heterogeneous among terminally differentiated single cells4-7. Here we introduce the multinucleic acid interaction mapping in single cells (MUSIC) technique for concurrent profiling of multiplex chromatin interactions, gene expression and RNA-chromatin associations within individual nuclei. When applied to 14 human frontal cortex samples from older donors, MUSIC delineated diverse cortical cell types and states. We observed that nuclei exhibiting fewer short-range chromatin interactions were correlated with both an older transcriptomic signature and Alzheimers disease pathology. Furthermore, the cell type exhibiting chromatin contacts between cis expression quantitative trait loci and a promoter tends to be that in which these cis expression quantitative trait loci specifically affect the expression of their target gene. In addition, female cortical cells exhibit highly heterogeneous interactions between XIST non-coding RNA and chromosome X, along with diverse spatial organizations of the X chromosomes. MUSIC presents a potent tool for exploration of chromatin architecture and transcription at cellular resolution in complex tissues.
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- 2024
11. Nuclear morphology is shaped by loop-extrusion programs
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Patta, Indumathi, Zand, Maryam, Lee, Lindsay, Mishra, Shreya, Bortnick, Alexandra, Lu, Hanbin, Prusty, Arpita, McArdle, Sara, Mikulski, Zbigniew, Wang, Huan-You, Cheng, Christine S, Fisch, Kathleen M, Hu, Ming, and Murre, Cornelis
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Biochemistry and Cell Biology ,Biological Sciences ,Genetics ,Cell Cycle Checkpoints ,Cell Cycle Proteins ,Cell Movement ,Chromatin ,Chromosomes ,Neutrophils ,Cell Nucleus Shape ,Nucleic Acid Conformation ,Cell Differentiation ,Inflammation ,Enhancer Elements ,Genetic ,Cell Lineage ,General Science & Technology - Abstract
It is well established that neutrophils adopt malleable polymorphonuclear shapes to migrate through narrow interstitial tissue spaces1-3. However, how polymorphonuclear structures are assembled remains unknown4. Here we show that in neutrophil progenitors, halting loop extrusion-a motor-powered process that generates DNA loops by pulling in chromatin5-leads to the assembly of polymorphonuclear genomes. Specifically, we found that in mononuclear neutrophil progenitors, acute depletion of the loop-extrusion loading factor nipped-B-like protein (NIPBL) induced the assembly of horseshoe, banded, ringed and hypersegmented nuclear structures and led to a reduction in nuclear volume, mirroring what is observed during the differentiation of neutrophils. Depletion of NIPBL also induced cell-cycle arrest, activated a neutrophil-specific gene program and conditioned a loss of interactions across topologically associating domains to generate a chromatin architecture that resembled that of differentiated neutrophils. Removing NIPBL resulted in enrichment for mega-loops and interchromosomal hubs that contain genes associated with neutrophil-specific enhancer repertoires and an inflammatory gene program. On the basis of these observations, we propose that in neutrophil progenitors, loop-extrusion programs produce lineage-specific chromatin architectures that permit the packing of chromosomes into geometrically confined lobular structures. Our data also provide a blueprint for the assembly of polymorphonuclear structures, and point to the possibility of engineering de novo nuclear shapes to facilitate the migration of effector cells in densely populated tumorigenic environments.
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- 2024
12. Chromosome-level genome of the transformable northern wattle, Acacia crassicarpa.
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Massaro, Isabelle, Poethig, Richard, Leichty, Aaron, and Sinha, Neelima
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Acacia crassicarpa ,Acra3RX ,Caesalpinioideae ,northern wattle ,transformation ,unifacial morphogenesis ,whole-genome duplication ,Animals ,Acacia ,Comb and Wattles ,Base Sequence ,Chromosomes - Abstract
The genus Acacia is a large group of woody legumes containing an enormous amount of morphological diversity in leaf shape. This diversity is at least in part the result of an innovation in leaf development where many Acacia species are capable of developing leaves of both bifacial and unifacial morphologies. While not unique in the plant kingdom, unifaciality is most commonly associated with monocots, and its developmental genetic mechanisms have yet to be explored beyond this group. In this study, we identify an accession of Acacia crassicarpa with high regeneration rates and isolate a clone for genome sequencing. We generate a chromosome-level assembly of this readily transformable clone, and using comparative analyses, confirm a whole-genome duplication unique to Caesalpinoid legumes. This resource will be important for future work examining genome evolution in legumes and the unique developmental genetic mechanisms underlying unifacial morphogenesis in Acacia.
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- 2024
13. Whole-genome sequence and annotation of Penstemon davidsonii.
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Alabady, Magdy, Zhang, Mengrui, Rausher, Mark, and Ostevik, Kate
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Penstemon davidsonii ,Davidsons beardtongue ,Hi-C ,PacBio Hifi ,genome annotation ,genome assembly ,Penstemon ,Molecular Sequence Annotation ,Genome ,High-Throughput Nucleotide Sequencing ,Transcriptome ,Chromosomes - Abstract
Penstemon is the most speciose flowering plant genus endemic to North America. Penstemon species diverse morphology and adaptation to various environments have made them a valuable model system for studying evolution. Here, we report the first full reference genome assembly and annotation for Penstemon davidsonii. Using PacBio long-read sequencing and Hi-C scaffolding technology, we constructed a de novo reference genome of 437,568,744 bases, with a contig N50 of 40 Mb and L50 of 5. The annotation includes 18,199 gene models, and both the genome and transcriptome assembly contain over 95% complete eudicot BUSCOs. This genome assembly will serve as a valuable reference for studying the evolutionary history and genetic diversity of the Penstemon genus.
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- 2024
14. Its a competitive business.
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Fraser, Christopher and Sokabe, Masaaki
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chromosomes ,gene expression ,mRNA ,molecular biology ,purified reconstituted systems ,ribosomes ,translation ,Protein Biosynthesis ,Ribosomes ,RNA ,Messenger - Abstract
A new in vitro system called Rec-Seq sheds light on how mRNA molecules compete for the machinery that translates their genetic sequence into proteins.
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- 2024
15. Inhibition of Cpeb3 ribozyme elevates CPEB3 protein expression and polyadenylation of its target mRNAs and enhances object location memory.
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Chen, Claire, Han, Joseph, Chinn, Carlene, Rounds, Jacob, Li, Xiang, Nikan, Mehran, Myszka, Marie, Tong, Liqi, Passalacqua, Luiz, Bredy, Timothy, Wood, Marcelo, and Luptak, Andrej
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CPEB3 ,chromosomes ,gene expression ,mouse ,neuroplasticity ,neuroscience ,ribozyme ,Mice ,Humans ,Animals ,RNA ,Catalytic ,RNA ,Messenger ,Polyadenylation ,Memory ,Long-Term ,Neurons ,RNA-Binding Proteins - Abstract
A self-cleaving ribozyme that maps to an intron of the cytoplasmic polyadenylation element-binding protein 3 (Cpeb3) gene is thought to play a role in human episodic memory, but the underlying mechanisms mediating this effect are not known. We tested the activity of the murine sequence and found that the ribozymes self-scission half-life matches the time it takes an RNA polymerase to reach the immediate downstream exon, suggesting that the ribozyme-dependent intron cleavage is tuned to co-transcriptional splicing of the Cpeb3 mRNA. Our studies also reveal that the murine ribozyme modulates maturation of its harboring mRNA in both cultured cortical neurons and the hippocampus: inhibition of the ribozyme using an antisense oligonucleotide leads to increased CPEB3 protein expression, which enhances polyadenylation and translation of localized plasticity-related target mRNAs, and subsequently strengthens hippocampal-dependent long-term memory. These findings reveal a previously unknown role for self-cleaving ribozyme activity in regulating experience-induced co-transcriptional and local translational processes required for learning and memory.
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- 2024
16. A highly contiguous genome assembly for the pocket mouse Perognathus longimembris longimembris
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Kozak, Krzysztof M, Escalona, Merly, Chumchim, Noravit, Fairbairn, Colin, Marimuthu, Mohan PA, Nguyen, Oanh, Sahasrabudhe, Ruta, Seligmann, William, Conroy, Chris, Patton, James L, Bowie, Rauri CK, and Nachman, Michael W
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Biological Sciences ,Ecology ,Genetics ,Human Genome ,Life on Land ,Animals ,Mice ,Genome ,Chromosomes ,Genomics ,North America ,California Conservation Genomics Project ,comparative genomics ,conservation genetics ,Heteromyidae ,Perognathus ,repetitive elements ,Evolutionary Biology ,Evolutionary biology - Abstract
The little pocket mouse, Perognathus longimembris, and its nine congeners are small heteromyid rodents found in arid and seasonally arid regions of Western North America. The genus is characterized by behavioral and physiological adaptations to dry and often harsh environments, including nocturnality, seasonal torpor, food caching, enhanced osmoregulation, and a well-developed sense of hearing. Here we present a genome assembly of Perognathus longimembris longimembris generated from PacBio HiFi long read and Omni-C chromatin-proximity sequencing as part of the California Conservation Genomics Project. The assembly has a length of 2.35 Gb, contig N50 of 11.6 Mb, scaffold N50 of 73.2 Mb, and includes 93.8% of the BUSCO Glires genes. Interspersed repetitive elements constitute 41.2% of the genome. A comparison with the highly endangered Pacific pocket mouse, P. l. pacificus, reveals broad synteny. These new resources will enable studies of local adaptation, genetic diversity, and conservation of threatened taxa.
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- 2024
17. A chromosome-level genome assembly for the smoky rubyspot damselfly (Hetaerina titia)
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Patterson, Christophe W, Bonillas-Monge, Erandi, Brennan, Adrian, Grether, Gregory F, Mendoza-Cuenca, Luis, Tucker, Rachel, Vega-Sánchez, Yesenia M, and Drury, Jonathan
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Biological Sciences ,Genetics ,Animals ,Female ,Odonata ,Smoke ,Biological Evolution ,Pigmentation ,Chromosomes ,aquatic insect ,Calopterygidae ,comparative genomics ,long-read sequencing ,Omni-C ,PacBio ,riparian ,Zygoptera ,Evolutionary Biology ,Evolutionary biology - Abstract
Smoky rubyspot damselflies (Hetaerina titia Drury, 1773) are one of the most commonly encountered odonates along streams and rivers on both slopes of Central America and the Atlantic drainages in the United States and southern Canada. Owing to their highly variable wing pigmentation, they have become a model system for studying sexual selection and interspecific behavioral interference. Here, we sequence and assemble the genome of a female smoky rubyspot. Of the primary assembly (i.e. the principle pseudohaplotype), 98.8% is made up of 12 chromosomal pseudomolecules (2N = 22A + X). There are 75 scaffolds in total, an N50 of 120 Mb, a contig-N50 of 0.64 Mb, and a high arthropod BUSCO score [C: 97.6% (S: 97.3%, D: 0.3%), F: 0.8%, M: 1.6%]. We then compare our assembly to that of the blue-tailed damselfly genome (Ischnura elegans), the most complete damselfly assembly to date, and a recently published assembly for an American rubyspot damselfly (Hetaerina americana). Collectively, these resources make Hetaerina a genome-enabled genus for further studies of the ecological and evolutionary forces shaping biological diversity.
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- 2024
18. A haplotype-like, chromosome-level assembled and annotated genome of Biomphalaria glabrata, an important intermediate host of schistosomiasis and the best studied model of schistosomiasis vector snails.
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Zhong, Daibin, Bu, Lijing, Habib, Mohamed, Lu, Lijun, Yan, Guiyun, and Zhang, Si-Ming
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Humans ,Animals ,Biomphalaria ,Haplotypes ,Schistosomiasis ,Fibrinogen ,Hemostatics ,Chromosomes - Abstract
Schistosomiasis is one of the worlds most devastating parasitic diseases, afflicting 251 million people globally. The Neotropical snail Biomphalaria glabrata is an important intermediate host of the human blood fluke Schistosoma mansoni and a predominant model for schistosomiasis research. To fully exploit this model snail for biomedical research, here we report a haplotype-like, chromosome-level assembled and annotated genome of the homozygous iM line of B. glabrata that we developed at the University of New Mexico. Using multiple sequencing platforms, including Illumina, PacBio, and Omni-C sequencing, 18 sequence contact matrices representing 18 haploid chromosomes (2n = 36) were generated (337x genome coverage), and 96.5% of the scaffold sequences were anchored to the 18 chromosomes. Protein-coding genes (n = 34,559), non-coding RNAs (n = 2,406), and repetitive elements (42.52% of the genome) were predicted for the whole genome, and detailed annotations for individual chromosomes were also provided. Using this genomic resource, we have investigated the genomic structure and organization of the Toll-like receptor (TLR) and fibrinogen-domain containing protein (FReD) genes, the two important immune-related gene families. Notably, TLR-like genes are scattered on 13 chromosomes. In contrast, almost all (39 of 40) fibrinogen-related genes (FREPs) (immunoglobulin superfamily (IgSF) + fibrinogen (FBG)) are clustered within a 5-million nucleotide region on chromosome 13, yielding insight into mechanisms involved in the diversification of FREPs. This is the first genome of schistosomiasis vector snails that has been assembled at the chromosome level, annotated, and analyzed. It serves as a valuable resource for a deeper understanding of the biology of vector snails, especially Biomphalaria snails.
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- 2024
19. Analysis of five near-complete genome assemblies of the tomato pathogen Cladosporium fulvum uncovers additional accessory chromosomes and structural variations induced by transposable elements effecting the loss of avirulence genes.
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Zaccaron, Alex and Stergiopoulos, Ioannis
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Chromosome translocation ,Dispensable chromosome ,Effectors ,Gene loss ,Genome evolution ,Pangenome ,Pseudogenization ,Repeat-induced point mutations ,Transposons ,Two-speed genome ,Solanum lycopersicum ,DNA Transposable Elements ,Genes ,Fungal ,Cladosporium ,Plants ,Chromosomes ,Nucleotides ,Plant Diseases ,Fungal Proteins ,Ascomycota - Abstract
BACKGROUND: Fungal plant pathogens have dynamic genomes that allow them to rapidly adapt to adverse conditions and overcome host resistance. One way by which this dynamic genome plasticity is expressed is through effector gene loss, which enables plant pathogens to overcome recognition by cognate resistance genes in the host. However, the exact nature of these loses remains elusive in many fungi. This includes the tomato pathogen Cladosporium fulvum, which is the first fungal plant pathogen from which avirulence (Avr) genes were ever cloned and in which loss of Avr genes is often reported as a means of overcoming recognition by cognate tomato Cf resistance genes. A recent near-complete reference genome assembly of C. fulvum isolate Race 5 revealed a compartmentalized genome architecture and the presence of an accessory chromosome, thereby creating a basis for studying genome plasticity in fungal plant pathogens and its impact on avirulence genes. RESULTS: Here, we obtained near-complete genome assemblies of four additional C. fulvum isolates. The genome assemblies had similar sizes (66.96 to 67.78 Mb), number of predicted genes (14,895 to 14,981), and estimated completeness (98.8 to 98.9%). Comparative analysis that included the genome of isolate Race 5 revealed high levels of synteny and colinearity, which extended to the density and distribution of repetitive elements and of repeat-induced point (RIP) mutations across homologous chromosomes. Nonetheless, structural variations, likely mediated by transposable elements and effecting the deletion of the avirulence genes Avr4E, Avr5, and Avr9, were also identified. The isolates further shared a core set of 13 chromosomes, but two accessory chromosomes were identified as well. Accessory chromosomes were significantly smaller in size, and one carried pseudogenized copies of two effector genes. Whole-genome alignments further revealed genomic islands of near-zero nucleotide diversity interspersed with islands of high nucleotide diversity that co-localized with repeat-rich regions. These regions were likely generated by RIP, which generally asymmetrically affected the genome of C. fulvum. CONCLUSIONS: Our results reveal new evolutionary aspects of the C. fulvum genome and provide new insights on the importance of genomic structural variations in overcoming host resistance in fungal plant pathogens.
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- 2024
20. X-chromosome and kidney function: evidence from a multi-trait genetic analysis of 908,697 individuals reveals sex-specific and sex-differential findings in genes regulated by androgen response elements.
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Scholz, Markus, Horn, Katrin, Pott, Janne, Wuttke, Matthias, Kühnapfel, Andreas, Nasr, M, Kirsten, Holger, Li, Yong, Hoppmann, Anselm, Gorski, Mathias, Ghasemi, Sahar, Li, Man, Tin, Adrienne, Chai, Jin-Fang, Cocca, Massimiliano, Wang, Judy, Nutile, Teresa, Akiyama, Masato, Åsvold, Bjørn, Bansal, Nisha, Biggs, Mary, Boutin, Thibaud, Brenner, Hermann, Brumpton, Ben, Burkhardt, Ralph, Cai, Jianwen, Campbell, Archie, Campbell, Harry, Chalmers, John, Chasman, Daniel, Chee, Miao, Chen, Xu, Cheng, Ching-Yu, Cifkova, Renata, Daviglus, Martha, Delgado, Graciela, Dittrich, Katalin, Edwards, Todd, Endlich, Karlhans, Michael Gaziano, J, Giri, Ayush, Giulianini, Franco, Gordon, Scott, Gudbjartsson, Daniel, Hallan, Stein, Hamet, Pavel, Hartman, Catharina, Hayward, Caroline, Heid, Iris, Hellwege, Jacklyn, Holleczek, Bernd, Holm, Hilma, Hutri-Kähönen, Nina, Hveem, Kristian, Isermann, Berend, Jonas, Jost, Joshi, Peter, Kamatani, Yoichiro, Kanai, Masahiro, Kastarinen, Mika, Khor, Chiea, Kiess, Wieland, Kleber, Marcus, Körner, Antje, Kovacs, Peter, Krajcoviechova, Alena, Kramer, Holly, Krämer, Bernhard, Kuokkanen, Mikko, Kähönen, Mika, Lange, Leslie, Lash, James, Lehtimäki, Terho, Li, Hengtong, Lin, Bridget, Liu, Jianjun, Loeffler, Markus, Lyytikäinen, Leo-Pekka, Magnusson, Patrik, Martin, Nicholas, Matsuda, Koichi, Milaneschi, Yuri, Mishra, Pashupati, Mononen, Nina, Montgomery, Grant, Mook-Kanamori, Dennis, Mychaleckyj, Josyf, März, Winfried, Nauck, Matthias, Nikus, Kjell, Nolte, Ilja, Noordam, Raymond, Okada, Yukinori, Olafsson, Isleifur, Oldehinkel, Albertine, Penninx, Brenda, Perola, Markus, Pirastu, Nicola, and Polasek, Ozren
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Humans ,Male ,Female ,Androgens ,Genome-Wide Association Study ,Kidney ,Chromosomes ,Human ,X ,Response Elements ,Polymorphism ,Single Nucleotide ,Genetic Predisposition to Disease ,Tetraspanins - Abstract
X-chromosomal genetic variants are understudied but can yield valuable insights into sexually dimorphic human traits and diseases. We performed a sex-stratified cross-ancestry X-chromosome-wide association meta-analysis of seven kidney-related traits (n = 908,697), identifying 23 loci genome-wide significantly associated with two of the traits: 7 for uric acid and 16 for estimated glomerular filtration rate (eGFR), including four novel eGFR loci containing the functionally plausible prioritized genes ACSL4, CLDN2, TSPAN6 and the female-specific DRP2. Further, we identified five novel sex-interactions, comprising male-specific effects at FAM9B and AR/EDA2R, and three sex-differential findings with larger genetic effect sizes in males at DCAF12L1 and MST4 and larger effect sizes in females at HPRT1. All prioritized genes in loci showing significant sex-interactions were located next to androgen response elements (ARE). Five ARE genes showed sex-differential expressions. This study contributes new insights into sex-dimorphisms of kidney traits along with new prioritized gene targets for further molecular research.
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- 2024
21. A chromosome-level genome for the nudibranch gastropod Berghia stephanieae helps parse clade-specific gene expression in novel and conserved phenotypes.
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Goodheart, Jessica, Rio, Robin, Taraporevala, Neville, Fiorenza, Rose, Barnes, Seth, Morrill, Kevin, Jacob, Mark, Whitesel, Carl, Masterson, Park, Batzel, Grant, Johnston, Hereroa, Ramirez, M, Katz, Paul, and Lyons, Deirdre
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Differential gene expression ,Gastropoda ,Lineage-restricted genes ,Novelty ,Nudibranchia ,Animals ,Gastropoda ,Phylogeny ,Evolution ,Molecular ,Mollusca ,Chromosomes ,Phenotype ,Gene Expression - Abstract
BACKGROUND: How novel phenotypes originate from conserved genes, processes, and tissues remains a major question in biology. Research that sets out to answer this question often focuses on the conserved genes and processes involved, an approach that explicitly excludes the impact of genetic elements that may be classified as clade-specific, even though many of these genes are known to be important for many novel, or clade-restricted, phenotypes. This is especially true for understudied phyla such as mollusks, where limited genomic and functional biology resources for members of this phylum have long hindered assessments of genetic homology and function. To address this gap, we constructed a chromosome-level genome for the gastropod Berghia stephanieae (Valdés, 2005) to investigate the expression of clade-specific genes across both novel and conserved tissue types in this species. RESULTS: The final assembled and filtered Berghia genome is comparable to other high-quality mollusk genomes in terms of size (1.05 Gb) and number of predicted genes (24,960 genes) and is highly contiguous. The proportion of upregulated, clade-specific genes varied across tissues, but with no clear trend between the proportion of clade-specific genes and the novelty of the tissue. However, more complex tissue like the brain had the highest total number of upregulated, clade-specific genes, though the ratio of upregulated clade-specific genes to the total number of upregulated genes was low. CONCLUSIONS: Our results, when combined with previous research on the impact of novel genes on phenotypic evolution, highlight the fact that the complexity of the novel tissue or behavior, the type of novelty, and the developmental timing of evolutionary modifications will all influence how novel and conserved genes interact to generate diversity.
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- 2024
22. Beyond the Global Brain Differences: Intraindividual Variability Differences in 1q21.1 Distal and 15q11.2 BP1-BP2 Deletion Carriers.
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Boen, Rune, Kaufmann, Tobias, van der Meer, Dennis, Frei, Oleksandr, Agartz, Ingrid, Ames, David, Andersson, Micael, Armstrong, Nicola, Artiges, Eric, Atkins, Joshua, Bauer, Jochen, Benedetti, Francesco, Boomsma, Dorret, Brodaty, Henry, Brosch, Katharina, Buckner, Randy, Cairns, Murray, Calhoun, Vince, Caspers, Svenja, Cichon, Sven, Corvin, Aiden, Crespo-Facorro, Benedicto, Dannlowski, Udo, David, Friederike, de Geus, Eco, de Zubicaray, Greig, Desrivières, Sylvane, Doherty, Joanne, Donohoe, Gary, Ehrlich, Stefan, Eising, Else, Espeseth, Thomas, Fisher, Simon, Forstner, Andreas, Fortaner-Uyà, Lidia, Frouin, Vincent, Fukunaga, Masaki, Ge, Tian, Glahn, David, Goltermann, Janik, Grabe, Hans, Green, Melissa, Groenewold, Nynke, Grotegerd, Dominik, Grøntvedt, Gøril, Hahn, Tim, Hashimoto, Ryota, Hehir-Kwa, Jayne, Henskens, Frans, Holmes, Avram, Håberg, Asta, Haavik, Jan, Jacquemont, Sebastien, Jansen, Andreas, Jockwitz, Christiane, Jönsson, Erik, Kikuchi, Masataka, Kircher, Tilo, Kumar, Kuldeep, Le Hellard, Stephanie, Leu, Costin, Linden, David, Liu, Jingyu, Loughnan, Robert, Mather, Karen, McMahon, Katie, McRae, Allan, Medland, Sarah, Meinert, Susanne, Moreau, Clara, Morris, Derek, Mowry, Bryan, Mühleisen, Thomas, Nenadić, Igor, Nöthen, Markus, Nyberg, Lars, Ophoff, Roel, Owen, Michael, Pantelis, Christos, Paolini, Marco, Paus, Tomas, Pausova, Zdenka, Persson, Karin, Quidé, Yann, Marques, Tiago, Sachdev, Perminder, Sando, Sigrid, Schall, Ulrich, Scott, Rodney, Selbæk, Geir, Shumskaya, Elena, Silva, Ana, Sisodiya, Sanjay, Stein, Frederike, Stein, Dan, Straube, Benjamin, Streit, Fabian, Strike, Lachlan, Teumer, Alexander, and Teutenberg, Lea
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15q11.2 BP1-BP2 ,1q21.1 distal ,Brain structure ,Copy number variants ,Intraindividual variability ,Magnetic resonance imaging ,Humans ,Chromosome Deletion ,Brain ,Magnetic Resonance Imaging ,Abnormalities ,Multiple ,Chromosomes ,Human ,Pair 15 ,DNA Copy Number Variations - Abstract
BACKGROUND: Carriers of the 1q21.1 distal and 15q11.2 BP1-BP2 copy number variants exhibit regional and global brain differences compared with noncarriers. However, interpreting regional differences is challenging if a global difference drives the regional brain differences. Intraindividual variability measures can be used to test for regional differences beyond global differences in brain structure. METHODS: Magnetic resonance imaging data were used to obtain regional brain values for 1q21.1 distal deletion (n = 30) and duplication (n = 27) and 15q11.2 BP1-BP2 deletion (n = 170) and duplication (n = 243) carriers and matched noncarriers (n = 2350). Regional intra-deviation scores, i.e., the standardized difference between an individuals regional difference and global difference, were used to test for regional differences that diverge from the global difference. RESULTS: For the 1q21.1 distal deletion carriers, cortical surface area for regions in the medial visual cortex, posterior cingulate, and temporal pole differed less and regions in the prefrontal and superior temporal cortex differed more than the global difference in cortical surface area. For the 15q11.2 BP1-BP2 deletion carriers, cortical thickness in regions in the medial visual cortex, auditory cortex, and temporal pole differed less and the prefrontal and somatosensory cortex differed more than the global difference in cortical thickness. CONCLUSIONS: We find evidence for regional effects beyond differences in global brain measures in 1q21.1 distal and 15q11.2 BP1-BP2 copy number variants. The results provide new insight into brain profiling of the 1q21.1 distal and 15q11.2 BP1-BP2 copy number variants, with the potential to increase understanding of the mechanisms involved in altered neurodevelopment.
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- 2024
23. New Observations of the Effects of the Cytoplasm of Aegilops kotschyi Boiss. in Bread Wheat Triticum aestivum L.
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Fan, Chaolan, Melonek, Joanna, and Lukaszewski, Adam J
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Biological Sciences ,Genetics ,Human Genome ,Contraception/Reproduction ,1.1 Normal biological development and functioning ,Triticum ,Cytoplasm ,Aegilops ,Chromosomes ,Plant ,Haploidy ,Pollen ,Parthenogenesis ,Seeds ,Plant Infertility ,Cell Nucleus ,cytoplasmic male sterility ,fertility restoration ,parthenogenesis ,haploidy ,double fertilization - Abstract
The cytoplasm of Aegilops kotschyi is known for the induction of male sterility and haploidy in wheat. Both systems originally appeared rather simple, but manipulation of the standard chromosome constitution of the nuclear genome revealed additional interactions. This study shows that while there is little or no allelic variation at the main fertility restorer locus Rfmulti on chromosome arm 1BS, additional genes may also be involved in the nuclear-mitochondrial genome interactions, affecting not only male fertility but also the growth rate, from pollen competition for fertilization and early endosperm divisions all the way to seed size and plant maturity. Some of these effects appear to be of a sporophytic nature; others are gametophytic. Induction of parthenogenesis by a rye inducer in conjunction with the Ae. kotschyi cytoplasm is well known. However, here we show that the cytoplasmic-nuclear interactions affect all aspects of double fertilization: producing maternal haploids from unfertilized eggs, diploids from fertilized eggs or synergids, embryo-less kernels, and fertilized eggs without fertilization of the double nucleus in the embryo sack. It is unclear how frequent the inducers of parthenogenesis are, as variation, if any, is obscured by suppressors present in the wheat genome. Genetic dissection of a single wheat accession revealed five distinct loci affecting the rate of maternal haploid production: four acting as suppressors and one as an enhancer. Only when the suppressing haplotypes are confirmed may it be possible to the identify genetic variation of haploidy inducers, map their position(s), and determine their nature and the mode of action.
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- 2024
24. Sleep EEG signatures in mouse models of 15q11.2-13.1 duplication (Dup15q) syndrome
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Saravanapandian, Vidya, Madani, Melika, Nichols, India, Vincent, Scott, Dover, Mary, Dikeman, Dante, Philpot, Benjamin D, Takumi, Toru, Colwell, Christopher S, Jeste, Shafali, Paul, Ketema N, and Golshani, Peyman
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Medical Physiology ,Biomedical and Clinical Sciences ,Genetics ,Intellectual and Developmental Disabilities (IDD) ,Behavioral and Social Science ,Sleep Research ,Basic Behavioral and Social Science ,Pediatric ,Brain Disorders ,Mental Health ,Neurosciences ,2.1 Biological and endogenous factors ,1.1 Normal biological development and functioning ,Neurological ,Mental health ,Animals ,Mice ,Chromosomes ,Human ,Pair 15 ,Electroencephalography ,Disease Models ,Animal ,Male ,Female ,Sleep Wake Disorders ,Sleep ,Trisomy ,Chromosome Aberrations ,Intellectual Disability ,Dup15q syndrome ,Autism ,Biomarkers ,EEG ,GABA ,UBE3A ,Neurodevelopmental disorders ,Psychology - Abstract
BackgroundSleep disturbances are a prevalent and complex comorbidity in neurodevelopmental disorders (NDDs). Dup15q syndrome (duplications of 15q11.2-13.1) is a genetic disorder highly penetrant for NDDs such as autism and intellectual disability and it is frequently accompanied by significant disruptions in sleep patterns. The 15q critical region harbors genes crucial for brain development, notably UBE3A and a cluster of gamma-aminobutyric acid type A receptor (GABAAR) genes. We previously described an electrophysiological biomarker of the syndrome, marked by heightened beta oscillations (12-30 Hz) in individuals with Dup15q syndrome, akin to electroencephalogram (EEG) alterations induced by allosteric modulation of GABAARs. Those with Dup15q syndrome exhibited increased beta oscillations during the awake resting state and during sleep, and they showed profoundly abnormal NREM sleep. This study aims to assess the translational validity of these EEG signatures and to delve into their neurobiological underpinnings by quantifying sleep physiology in chromosome-engineered mice with maternal (matDp/ + mice) or paternal (patDp/ + mice) inheritance of the full 15q11.2-13.1-equivalent duplication, and mice with duplication of just the UBE3A gene (Ube3a overexpression mice; Ube3a OE mice) and comparing the sleep metrics with their respective wildtype (WT) littermate controls.MethodsWe collected 48-h EEG/EMG recordings from 35 (23 male, 12 female) 12-24-week-old matDp/ + , patDp/ + , Ube3a OE mice, and their WT littermate controls. We quantified baseline sleep, sleep fragmentation, spectral power dynamics during sleep states, and recovery following sleep deprivation. Within each group, distinctions between Dup15q mutant mice and WT littermate controls were evaluated using analysis of variance (ANOVA) and student's t-test. The impact of genotype and time was discerned through repeated measures ANOVA, and significance was established at p
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- 2024
25. Chromosome-scale genome assembly of bread wheat’s wild relative Triticum timopheevii
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Grewal, Surbhi, Yang, Cai-yun, Scholefield, Duncan, Ashling, Stephen, Ghosh, Sreya, Swarbreck, David, Collins, Joanna, Yao, Eric, Sen, Taner Z, Wilson, Michael, Yant, Levi, King, Ian P, and King, Julie
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Agricultural ,Veterinary and Food Sciences ,Biological Sciences ,Bioinformatics and Computational Biology ,Genetics ,Human Genome ,Generic health relevance ,Zero Hunger ,Triticum ,Genome ,Plant ,Chromosomes ,Plant ,DNA Methylation - Abstract
Wheat (Triticum aestivum) is one of the most important food crops with an urgent need for increase in its production to feed the growing world. Triticum timopheevii (2n = 4x = 28) is an allotetraploid wheat wild relative species containing the At and G genomes that has been exploited in many pre-breeding programmes for wheat improvement. In this study, we report the generation of a chromosome-scale reference genome assembly of T. timopheevii accession PI 94760 based on PacBio HiFi reads and chromosome conformation capture (Hi-C). The assembly comprised a total size of 9.35 Gb, featuring a contig N50 of 42.4 Mb and included the mitochondrial and plastid genome sequences. Genome annotation predicted 166,325 gene models including 70,365 genes with high confidence. DNA methylation analysis showed that the G genome had on average more methylated bases than the At genome. In summary, the T. timopheevii genome assembly provides a valuable resource for genome-informed discovery of agronomically important genes for food security.
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- 2024
26. A chromosome level reference genome of Diviner's sage (Salvia divinorum) provides insight into salvinorin A biosynthesis.
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Ford, Scott A., Ness, Rob W., Kwon, Moonhyuk, Ro, Dae-Kyun, and Phillips, Michael A.
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GENE expression , *POST-traumatic stress disorder , *SAGE , *MEDICINAL plants , *CHROMOSOMES , *OPIOID receptors - Abstract
Background: Diviner's sage (Salvia divinorum; Lamiaceae) is the source of the powerful hallucinogen salvinorin A (SalA). This neoclerodane diterpenoid is an agonist of the human Κ-opioid receptor with potential medical applications in the treatment of chronic pain, addiction, and post-traumatic stress disorder. Only two steps of the approximately twelve step biosynthetic sequence leading to SalA have been resolved to date. Results: To facilitate pathway elucidation in this ethnomedicinal plant species, here we report a chromosome level genome assembly. A high-quality genome sequence was assembled with an N50 value of 41.4 Mb and a BUSCO completeness score of 98.4%. The diploid (2n = 22) genome of ~ 541 Mb is comparable in size and ploidy to most other members of this genus. Two diterpene biosynthetic gene clusters were identified and are highly enriched in previously unidentified cytochrome P450s as well as crotonolide G synthase, which forms the dihydrofuran ring early in the SalA pathway. Coding sequences for other enzyme classes with likely involvement in downstream steps of the SalA pathway (BAHD acyl transferases, alcohol dehydrogenases, and O-methyl transferases) were scattered throughout the genome with no clear indication of clustering. Differential gene expression analysis suggests that most of these genes are not inducible by methyl jasmonate treatment. Conclusions: This genome sequence and associated gene annotation are among the highest resolution in Salvia, a genus well known for the medicinal properties of its members. Here we have identified the cohort of genes responsible for the remaining steps in the SalA pathway. This genome sequence and associated candidate genes will facilitate the elucidation of SalA biosynthesis and enable an exploration of its full clinical potential. [ABSTRACT FROM AUTHOR]
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- 2024
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27. Novel LOXL3-associated stickler syndrome-like phenotype: a case report.
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Klejnotowska, Adrianna E., Higgins, Megan, and Shah, Shaheen P.
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SKELETAL dysplasia , *VISUAL acuity , *MYOPIA , *PHENOTYPES , *CHROMOSOMES , *DYSPLASIA - Abstract
Purpose: To report the case of a young boy with early onset high myopia (eoHM), foveal hypoplasia and skeletal dysplasia due to a homozygous LOXL3 pathogenic variant. Atypically, this was from a paternal uniparental isodisomy (UPiD) of chromosome 2. Clinical Case: Four-year-old boy with several months history of holding items close to his face was found to have reduced visual acuity 6/30 in both eyes, bilateral vitreous syneresis, foveal hypoplasia and bilateral high myopia (−8.50D). A skeletal survey showed spondylo-epi-metaphyseal dysplasia. Whole-exome sequencing (WES) revealed a homozygous LOXL3 variant c.1448_1449del, p.(Thr483Argfs*13), inherited through paternal UPiD of chromosome 2. Conclusion: To our knowledge, this is the first reported case of LOXL3-associated eoHM, foveal hypoplasia and mild skeletal dysplasia due to the rare phenomenon of paternal UPiD of chromosome 2. This case further delineates the phenotype associated with LOXL3 pathogenic variants and supports truncating LOXL3 pathogenic variants being associated with a phenotypic spectrum; from isolated eoHM through to a Stickler syndrome-like phenotype. [ABSTRACT FROM AUTHOR]
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- 2024
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28. Somatic mutations in arteriovenous malformations in hereditary hemorrhagic telangiectasia support a bi-allelic two-hit mutation mechanism of pathogenesis.
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DeBose-Scarlett, Evon, Ressler, Andrew K., Gallione, Carol J., Sapisochin Cantis, Gonzalo, Friday, Cassi, Weinsheimer, Shantel, Schimmel, Katharina, Spiekerkoetter, Edda, Kim, Helen, Gossage, James R., Faughnan, Marie E., and Marchuk, Douglas A.
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SOMATIC mutation , *HEREDITARY hemorrhagic telangiectasia , *ARTERIOVENOUS malformation , *PHENOTYPES , *CHROMOSOMES , *CEREBRAL arteriovenous malformations - Abstract
Hereditary hemorrhagic telangiectasia (HHT) is an inherited disorder of vascular malformations characterized by mucocutaneous telangiectases and arteriovenous malformations (AVMs) in internal organs. HHT is caused by inheritance of a loss of function mutation in one of three genes. Although individuals with HHT are haploinsufficient for one of these genes throughout their entire body, rather than exhibiting a systemic vascular phenotype, vascular malformations occur as focal lesions in discrete anatomic locations. The inconsistency between genotype and phenotype has provoked debate over whether haploinsufficiency or a different mechanism gives rise to the vascular malformations. We previously showed that HHT-associated skin telangiectases develop by a two-hit mutation mechanism in an HHT gene. However, somatic mutations were identified in only half of the telangiectases, raising the question whether a second-hit somatic mutation is a necessary (required) event in HHT pathogenesis. Here, we show that another mechanism for the second hit is loss of heterozygosity across the chromosome bearing the germline mutation. Secondly, we investigate the two-hit mutation mechanism for internal organ AVMs, the source of much of the morbidity of HHT. Here, we identified somatic molecular genetic events in eight liver telangiectases, including point mutations and a loss of heterozygosity event. We also identified somatic mutations in one pulmonary AVM and two brain AVMs, confirming that mucocutaneous and internal organ vascular malformations undergo the same molecular mechanisms. Together, these data argue that bi-allelic loss of function in an HHT gene is a required event in the pathogenesis of HHT-associated vascular malformations. Hereditary hemorrhagic telangiectasia is an autosomal-dominant disorder consisting of focal vascular malformations. We show that some malformations acquire a somatic mutation in trans with the germline mutation, leading to bi-allelic gene loss. Others exhibit loss of heterozygosity across the chromosome bearing the germline mutation, supporting a two-hit mutation mechanism of pathogenesis. [ABSTRACT FROM AUTHOR]
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- 2024
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29. Permeable TAD boundaries and their impact on genome‐associated functions.
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Chang, Li‐Hsin and Noordermeer, Daan
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COHESINS , *BINDING sites , *GENETIC regulation , *CHROMATIN , *CHROMOSOMES - Abstract
TAD boundaries are genomic elements that separate biological processes in neighboring domains by blocking DNA loops that are formed through Cohesin‐mediated loop extrusion. Most TAD boundaries consist of arrays of binding sites for the CTCF protein, whose interaction with the Cohesin complex blocks loop extrusion. TAD boundaries are not fully impermeable though and allow a limited amount of inter‐TAD loop formation. Based on the reanalysis of Nano‐C data, a multicontact Chromosome Conformation Capture assay, we propose a model whereby clustered CTCF binding sites promote the successive stalling of Cohesin and subsequent dissociation from the chromatin. A fraction of Cohesin nonetheless achieves boundary read‐through. Due to a constant rate of Cohesin dissociation elsewhere in the genome, the maximum length of inter‐TAD loops is restricted though. We speculate that the DNA‐encoded organization of stalling sites regulates TAD boundary permeability and discuss implications for enhancer–promoter loop formation and other genomic processes. [ABSTRACT FROM AUTHOR]
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- 2024
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30. In through the out door: A loop‐binding‐first model for topological cohesin loading.
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Rhind, Nicholas
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HOMOLOGOUS recombination , *HOMOLOGOUS chromosomes , *COHESINS , *CHROMOSOMES , *INTERPHASE - Abstract
Cohesin is a ring‐shaped complex that is loaded on DNA in two different conformations. In one conformation, it forms loops to organize the interphase genome; in the other, it topologically encircles sibling chromosomes to facilitate homologous recombination and to establish the cohesion that is required for orderly segregation during mitosis. How, and even if, these two loading conformation are related is unclear. Here, I propose that loop binding is a required first step for topological binding. This loop‐binding‐first model integrates the known information about the two loading mechanisms, explains genetic requirements for the two and explains how topological loading evolved from loop binding. [ABSTRACT FROM AUTHOR]
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- 2024
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31. Lectotypification and taxonomy of the Italian endemic Biscutella incana (Brassicaceae).
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Bartolucci, F. and Conti, F.
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BOTANY , *BRASSICACEAE , *PHENOLOGY , *CHROMOSOMES , *TAXONOMY - Abstract
Biscutella incana is endemic to the southern Apennine Peninsula (Italy) and was first described by Michele Tenore in 1826 from Calabria. It belongs to B. ser. Levigatae, the most morphologically diversified and critical series within the genus. In order to fix the application of this name, a lectotype housed in NAP was designated here. An updated and detailed morphological description, distribution and information about habitat and phenology are provided. Furthermore, a chromosome count made on a new population discovered in Dolomiti Lucane (Basilicata, southern Italy), confirmed that B. incana is diploid (2n = 18). [ABSTRACT FROM AUTHOR]
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- 2024
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32. Functional characterization of KCNMA1 mutation associated with dyskinesia, seizure, developmental delay, and cerebellar atrophy.
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Yucesan, Emrah, Goncu, Beyza, Ozgul, Cemil, Kebapci, Arda, Aslanger, Ayca Dilruba, Akyuz, Enes, and Yesil, Gozde
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GAIN-of-function mutations , *CALCIUM-dependent potassium channels , *DEVELOPMENTAL delay , *CELL physiology , *CHROMOSOMES - Abstract
KCNMA1 located on chromosome 10q22.3, encodes the pore-forming α subunit of the 'Big K+' (BK) large conductance calcium and voltage-activated K + channel. Numerous evidence suggests the functional BK channel alterations produced by different KCNMA1 alleles may associate with different symptoms, such as paroxysmal non kinesigenic dyskinesia with gain of function and ataxia with loss of function. Functional classifications revealed two major patterns, gain of function and loss of function effects on channel properties in different cell lines. In the literature, two mutations have been shown to confer gain of function properties to BK channels: D434G and N995S. In this study, we report the functional characterization of a variant which was previously reported the whole exome sequencing revealed bi-allelic nonsense variation of the cytoplasmic domain of calcium-activated potassium channel subunit alpha-1 protein. To detect functional consequences of the variation, we parallely conducted two independent approaches. One is immunostaining using and the other one is electrophysiological recording using patch-clamp on wild-type and R458X mutant cells to detect the differences between wild-type and the mutant cells. We detected the gain of function effect for the mutation (NM_001161352.1 (ENST00000286628.8):c.1372C > T;Arg458*) using two parallel approaches. According to the result we found, the reported mutation causes the loss of function in the cell. It should be noted that in future studies, it can be thought that the functions of genes associated with channelopathies may have a dual effect such as loss and gain. [ABSTRACT FROM AUTHOR]
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- 2024
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33. The interplay between X-chromosome functional dosage and circadian regulation in females.
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Moysés-Oliveira, Mariana, Andersen, Monica L., and Tufik, Sergio
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HYPOTHALAMUS physiology , *RESEARCH funding , *SEX distribution , *GENETIC markers , *INSOMNIA , *MENTAL illness , *FISHER exact test , *DESCRIPTIVE statistics , *DISEASE prevalence , *GENE expression , *CHROMOSOMES , *CIRCADIAN rhythms , *SLEEP , *WOMEN'S health , *COMPARATIVE studies , *SLEEP disorders - Abstract
Purpose: Biological factors and mechanisms that drive higher prevalence of insomnia in females are poorly understood. This study focused on the neurological consequences of X-chromosome functional imbalances between sexes. Methods: Benefited from publicly available large-scale genetic, transcriptional and epigenomic data, we curated and contrasted different gene lists: (1) X-liked genes, including assignments for X-chromosome inactivation patterns and disease associations; (2) sleep-associated genes; (3) gene expression markers for the suprachiasmatic nucleus. Results: We show that X-linked markers for the suprachiasmatic nucleus are significantly enriched for clinically relevant genes in the context of rare genetic syndromes and brain waves modulation. Conclusion: Considering female-specific patterns on brain transcriptional programs becomes essential when designing health care strategies for mental and sleep illnesses with sex bias in prevalence. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
34. Whole genome sequence and comparative genomic analysis of novel Rickettsia koreansis strain CNH17-7 isolated from human.
- Author
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Kang, Taeuk, Choi, Yeon-Joo, Kim, Jeoungyeon, Park, Hye-Jin, and Jang, Won-Jong
- Subjects
- *
WHOLE genome sequencing , *GENOMICS , *RICKETTSIA , *ARITHMETIC mean , *CHROMOSOMES - Abstract
Purpose: To determine the genomic feature of novel spotted fever-causing Rickettsia koreansis strain CNH17-7, which is different from R. japonica that is a causative agent for Japanese spotted fever (JSF), and to perform its comparative genomic analysis. Methods: Whole genome sequencing (WGS) was performed on R. koreansis strain CNH17-7 by using the Illumina Miseq system. After WGS, assembly and annotation were done by SPAdes. Then, its genomic features were compared with 19 different Rickettsia species. Based on the average nucleotide identity (ANI) value, an unweighted pair group method with an arithmetic mean (UPGMA) dendrogram was generated. Following the dendrogram analysis, pan-and core-genome analysis was performed. Then additional comparative analyses with two genetically closest Rickettsia species were conducted based on gene repertoire. Results: R. koreansis strain CNH17-7 has a chromosome consisting of 1,392,633 bp with GC content of 32.4%. The ANI-derived UPGMA showed that R. koreansis strain CNH17-7 is genetically close to R. japonica YH and R. heilongjiangensis 054 but is distinctively differentiated. The ANI value of R. koreansis strain CNH17-7 to R. japonica YH and R. heilongjiangensis 054 are 98.14% and 98.04% respectively, indicating R. koreansis strain CNH17-7 is sufficient to be classified as a new species. Other than ANI, R. koreansis strain CNH17-7 also contains novel CDS and its COG functional category proportion which is distinct compared to R. japonica YH and R. heilongjiangensis 054. Conclusion: We have revealed genomic features of the novel R. koreansis strain CNH17-7. Hence, we propose R. koreansis strain CNH17-7 as new Rickettsia species. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
35. Insights into a functional synthetic plant genome.
- Author
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Du, Fei, Dai, Junbiao, and Jiao, Yuling
- Subjects
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ARTIFICIAL chromosomes , *PLANT genomes , *CHROMOSOMES , *GENOMICS , *MULTICELLULAR organisms , *EUKARYOTIC genomes - Abstract
Summary: Synthetic genomics involves the design, assembly, and transfer of artificially synthesized DNA fragments into target hosts to replace the native genome and construct viable forms of life. With advances in DNA synthesis and assembly techniques, the application of synthetic genomics in viruses, bacteria, and yeast has improved our knowledge of genome organization and function. Multicellular eukaryotic organisms are characterized by larger genomes, more complex epigenetic regulation, and widespread transposable elements, making genome synthesis challenging. Recently, the first synthetic multicellular eukaryotic organism was generated in the model plant Physcomitrium patens with a partially synthetic chromosome arm. Here, we introduce the design and assembly principles of moss genome synthesis. We also discuss the remaining technical barriers in the application of synthetic genomics in seed plants. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
36. Phylogenetic Inference from 16S rRNA Gene Sequencing in Chromosome Races of the Genus Nannospalax Species (Rodentia: Spalacidae).
- Author
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Teoman Kankılıç, Çelikbilek, Habibe Didem, Kankılıç, Tolga, Şeker, Perinçek Seçkinozan, Selvi, Engin, and Civelek, İlkay
- Subjects
- *
NAKED mole rat , *CHROMOSOMES , *PHYLOGENY , *RIBOSOMAL RNA , *RODENTS - Abstract
In this study, we aimed to develop a species-level phylogeny for the Nannospalax genus, identify cryptic species that are difficult to differentiate using standard methods, and further evaluate the relationships across chromosomal forms of several species. 16S rRNA gene sequences from 81 Turkish regions were evaluated and statistical analyzes were conducted. Results are as follows; (i) Nannospalax cilicicus was found to be monophyletic and sister in relation to Nannospalax xanthodon; (ii) We suggested for the first time that the Central-Anatolian populations should be classified under the species name Nannospalax cilicicus (stat. n.); (iii) the unidentified 2n = 52 cytotypes from Bolu and Nannospalax leucodon cytotypes were reciprocally monophyletic, these data support the notion that the 2n = 52 cytotypes from Bolu belong to an unidentified taxon; (iv) study also offered evidence for the monophyly of N. tuncelicus for the first time; (v) Nannospalax xanthodon (2n = 36, 38, and 40) and Nannospalax nehringi composed several species-specific clades, which form polytomy structure, so relationships between these species are still unclear. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
37. Can species endure massive introgression? Genomic evidence of asymmetric gene flow in Melitaea butterflies.
- Author
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Hinojosa, Joan C., Marques, Valéria, Mesa, Luis Sánchez, Dapporto, Leonardo, Dincă, Vlad, and Vila, Roger
- Subjects
- *
SPECIES hybridization , *NYMPHALIDAE , *PHENOTYPES , *CHROMOSOMES , *LEPIDOPTERA , *GENE flow - Abstract
Hybridisation and introgression are increasingly seen as important drivers of the evolution of organisms, particularly in Lepidoptera. One group that is gaining attention due to recently published cases of interspecific gene flow is the genus Melitaea Fabricius (Nymphalidae). In this study, we used genomics to investigate the role of hybridisation in the evolution of the western Palearctic species of the Melitaea phoebe group M. ornata Christoph, the recently described M. pseudornata Muñoz Sariot & Sánchez Mesa, M. phoebe (Denis & Schiffermüller), M. punica Oberthür, and M. telona (Fruhstorfer). We provide evidence of asymmetric gene flow from M. phoebe to both M. ornata and M. pseudornata. Gene flow from M. phoebe to M. pseudornata was very high (25.0%–31.9%), widespread throughout the distribution of the latter, and not equally distributed along the genome. The Z chromosome showed patterns compatible with the large‐Z effect, which were mimicked by two autosomes. Melitaea pseudornata endured massive introgression while remaining a separate entity from M. phoebe, although gene flow may have altered its phenotype, including its voltinism and the morphology of the adults and caterpillars. These findings suggest that hybridisation may be pervasive in this genus and highlight its key role in the evolution of butterflies, emphasising the need for further research on this topic. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
38. Runs of homozygosity analysis for selection signatures in the Yellow Korean native chicken.
- Author
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Jaewon Kim, Macharia, John Kariuki, Minjun Kim, Jung Min Heo, Myunghwan Yu, Hyo Jun Choo, and Jun Heon Lee
- Subjects
- *
LOCUS (Genetics) , *SINGLE nucleotide polymorphisms , *CHICKENS , *INBREEDING , *CHROMOSOMES , *POULTRY breeding - Abstract
Objective: Yellow Korean native chicken (KNC-Y) is one of the five pure Korean indigenous chicken breeds that were restored through a government project in 1992. KNC-Y is recognized for its superior egg production performance compared to other KNC lines. In this study, we performed runs of homozygosity (ROH) analysis to discover selection signatures associated with egg production traits in the KNC-Y population. Methods: A total of 675 DNA samples from KNC-Y were genotyped to generate single nucleotide polymorphism (SNP) data using custom 60K Affymetrix SNP chips. ROH analysis was performed using PLINK software, with predefined parameters set for the analysis. The threshold of ROH island was defined as the top 1% frequency of SNPs withing the ROH among the population. Results: In the KNC-Y population, a total of 29,958 runs of homozygosity (ROH) fragments were identified. The average total length of ROH was 120.84 Mb, with each ROH fragment having an average length of 2.71 Mb. The calculated ROH-based inbreeding coefficient (FROH) was 0.13. Furthermore, we revealed the presence of ROH islands on chromosomes 1, 2, 4, 5, 7, 8, and 11. Within the identified regions, a total of 111 genes were annotated, and among them were genes related to economic traits, including PRMT3, ANO5, HDAC4, LSS, PLA2G4A, and PTGS2. Most of the overlapping quantitative trait locus regions with ROH islands were found to be associated with production traits. Conclusion: This study conducted a comprehensive analysis of ROH in the KNC-Y population. Notably, among the findings, the PTGS2 gene is believed to play a crucial role in influencing the laying performance of KNC-Y. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
39. Myeloid Ecotropic Viral Integration Site (MEIS) Proteins: Illuminating Cancer Diagnosis and Biomarker Significance.
- Author
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Babaeizad, Ali, Yousefi, Bahman, Banihashemian, Seyedeh Zahra, Feyzabadi, Zahra Khatibiyan, and Eslami, Majid
- Subjects
TUMOR diagnosis ,HOMEOSTASIS ,EPIGENOMICS ,CELL physiology ,TUMOR markers ,TRANSCRIPTION factors ,CELLULAR signal transduction ,COLORECTAL cancer ,GENE expression ,LEUKEMIA ,CHROMOSOMES ,RENAL cell carcinoma ,CELL differentiation ,GENETIC mutation ,STEM cells ,DNA-binding proteins ,CHEMICAL inhibitors - Abstract
Context: The MEIS proteins, originally described as the "helper groups" of HOX proteins, are now becoming recognized as significant and incredibly diverse controllers of cellular behavior. The MEIS agents can incorporate a variety of epigenetic modulators into chromatin to aid in embryonic growth or tissue homeostasis by adult stem cells. The MEIS gene's expression, binding, stability, post-translational protein modification, and controlled intracellular availability are all fully regulated, and they all react to external stimuli. Objectives: We explored the diverse roles of MEIS proteins in cellular behavior and epigenetic regulation and investigated the potential of MEIS proteins as diagnostic and therapeutic biomarkers in cancers. Results: The MEIS proteins can be diagnostic and therapeutic biomarkers for cancer. The MEIS-PBX heterotrimeric is involved in neurogenesis processes, and the formation of the MEIS2 heterodimer with PAX6 and DLX2 can proliferate periplomerular dopaminergic neurons in the olfactory bulb. The estrogen receptor in breast cancer upregulates MEIS1 and FOXP3. The estrogen receptor pathway's cancer-related genes can express themselves more strongly when MEIS1-FOXP3 interacts with a positive feedback mechanism. Because MEIS1 expression is decreased in clear renal cell carcinoma (ccRCC) cell lines, MEIS1 may function as a tumor suppressor in the development of ccRCC. Conclusions: MEIS1 is recognized as one of the main contributors to the development of leukemias. The MEIS proteins influence the c-MYC signaling pathway, cell proliferation, and invasion of PC by contributing to its development. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
40. Chromosome Number Variation Along with Modest Morphological and Biochemical Differentiation among Wild Cyclamen Accessions (Myrsinaceae) in Iran.
- Author
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Kondori, Afsaneh Yavari, Naderi, Roohangiz, Khosrowshali, Mahmood, and Larijani, Kambiz
- Subjects
PLOIDY ,CYCLAMEN ,MYRSINACEAE ,CHROMOSOMES ,CLUSTER analysis (Statistics) - Abstract
Cyclamen, a popular decorative plant, is commonly found in gardens and pots. Since wild Cyclamen are endangered, a study was conducted to explore the genetic potential of wild Cyclamen, focusing on their morphology and chromosomal diversity among eight accessions. A total of 23 phenotypic-biochemical traits were evaluated along with chromosome counting. The morphological measures showed a humble range of variation, particularly in the number of flowers, peduncle diameter, number of leaves, hypocotyl length, and hypocotyl weight, with the Kordkuy accession being the largest and the Deylaman accession the most seeded. Cyclamen chromosome counting indicated 15 chromosomal levels, implying a significant chromosomal diversity among the accessions, with the Pasand accession exhibiting the highest level of chromosomal diversity (14 chromosomal levels). Furthermore, only the Pasand accession had the greatest chromosomal level (2n=48) among the accessions. By describing the results based on the median, the smallest chromosomal diversity was recorded in the Kordkuy accession. Cyclamen accessions were divided into two main clusters and a single accession. The Kordkuy accession was separated from the other accessions. HCA analysis confirmed the separation of the Kordkuy accession from the other accessions, as expected from its superiority in most morphological traits. The current findings can be used for improving Cyclamen breeding programs as well as evolutionary studies. We suggest more focus on the utilization of wild genetic resources to conserve Cyclamen varieties and strengthen their genetic background. [ABSTRACT FROM AUTHOR]
- Published
- 2024
41. Identification of quantitative trait loci and candidate genes for pod shatter resistance in Brassica carinata.
- Author
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Raman, Rosy, Zhang, Zun Xu, Diffey, Simon, Qiu, Yu, Niu, Yan, Zou, Jun, and Raman, Harsh
- Subjects
- *
LOCUS (Genetics) , *GENETIC variation , *SEED yield , *CHROMOSOMES , *GENE mapping - Abstract
Background: Understanding the genetic control of pod shatter resistance and its association with pod length is crucial for breeding improved pod shatter resistance and reducing pre-harvest yield losses due to extensive shattering in cultivars of Brassica species. In this study, we evaluated a doubled haploid (DH) mapping population derived from an F1 cross between two Brassica carinata parental lines Y-BcDH64 and W-BcDH76 (YWDH), originating from Ethiopia and determined genetic bases of variation in pod length and pod shatter resistance, measured as rupture energy. The YWDH population, its parental lines and 11 controls were grown across three years for genetic analysis. Results: By using three quantitative trait loci (QTL) analytic approaches, we identified nine genomic regions on B02, B03, B04, B06, B07 and C01 chromosomes for rupture energy that were repeatedly detected across three growing environments. One of the QTL on chromosome B07, flanked with DArTseq markers 100,046,735 and 100,022,658, accounted for up to 27.6% of genetic variance in rupture energy. We observed no relationship between pod length and rupture energy, suggesting that pod length does not contribute to variation in pod shatter resistance. Comparative mapping identified six candidate genes; SHP1 on B6, FUL and MAN on chromosomes B07, IND and NST2 on B08, and MAN7 on C07 that mapped within 0.2 Mb from the QTL for rupture energy. Conclusion: The results suggest that favourable alleles of stable QTL on B06, B07, B08 and C01 for pod shatter resistance can be incorporated into the shatter-prone B. carinata and its related species to improve final seed yield at harvest. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
42. Local volume concentration, packing domains, and scaling properties of chromatin.
- Author
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Carignano, Marcelo A., Kroeger, Martin, Almassalha, Luay M., Agrawal, Vasundhara, Wing Shun Li, Pujadas-Liwag, Emily M., Nap, Rikkert J., Backman, Vadim, and Szleifer, Igal
- Subjects
- *
CHROMOSOMES , *MULTIPLICITY (Mathematics) , *TOPOLOGY , *PROBABILITY theory , *SELF - Abstract
We propose the Self Returning Excluded Volume (SR-EV) model for the structure of chromatin based on stochastic rules and physical interactions. The SR-EV rules of return generate conformationally defined domains observed by single-cell imaging techniques. From nucleosome to chromosome scales, the model captures the overall chromatin organization as a corrugated system, with dense and dilute regions alternating in a manner that resembles the mixing of two disordered bi-continuous phases. This particular organizational topology is a consequence of the multiplicity of interactions and processes occurring in the nuclei, and mimicked by the proposed return rules. Single configuration properties and ensemble averages show a robust agreement between theoretical and experimental results including chromatin volume concentration, contact probability, packing domain identification and size characterization, and packing scaling behavior. Model and experimental results suggest that there is an inherent chromatin organization regardless of the cell character and resistant to an external forcing such as RAD21 degradation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
43. Integrated genomic, transcriptomic, and metabolomic analyses of Ilex hylonoma provide insights into the triterpenoid saponin biosynthesis.
- Author
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Feng, Landi, Yao, Yingjun, Kang, Minghui, Yang, Wengjie, Han, Yu, Liu, Wei, Li, Xiaonan, Li, Na, Hu, Yongqi, Liu, Jianquan, and Hu, Quanjun
- Subjects
- *
TRITERPENOID saponins , *GENE expression , *METABOLITES , *GENETIC regulation , *CHROMOSOMES , *SAPONINS - Abstract
SUMMARY Ilex is known for its rich content of secondary metabolites, particularly triterpenoid saponins. These compounds hold significant value in natural remedies and herbal medicine. However, the molecular mechanisms responsible for triterpenoid biosynthesis in plants of this genus remain largely unexplored. In this study, we successfully generated the first chromosome‐scale genome of Ilex hylonoma. The assembly, comprising 20 anchored chromosomes, has an N50 contig size of 2.13 Mb and a scaffold size of 33.68 Mb. Comparative genome analyses with two other congeners with available chromosome‐level genomes suggested that an end‐to‐end chromosome fusion event likely contributed to the reduction in chromosome number from n = 20 to n = 19 within this genus. By integrating transcriptomic and metabolomic data, we identified the gene expression patterns and metabolite profiles of I. hylonoma across three commonly utilized medicinal tissues. We subsequently pinpointed candidate genes involved in the regulation of triterpenoid saponin biosynthesis, including CYP450 genes, UGT genes, and associated transcription factors. Furthermore, yeast heterologous expression analysis revealed that ihyl08363 catalyzed the conversion of β‐amyrin into oleanolic acid, while ihyl04303 catalyzed the C‐2α hydroxylation of oleanolic acid to produce maslinic acid. This integrated analysis provides valuable insights into the biosynthesis of important triterpenoid saponins in medicinal Ilex plants. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
44. Bridging the gap: unravelling plant centromeres in the telomere‐to‐telomere era.
- Author
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Naish, Matthew
- Subjects
- *
CHROMOSOME segregation , *SATELLITE DNA , *KINETOCHORE , *CHROMATIN , *CHROMOSOMES , *CENTROMERE - Abstract
Summary Centromeres are specific regions of the chromosomes that play a pivotal role in the segregation of chromosomes, by facilitating the loading of the kinetochore, which forms the link between the chromosomes to the spindle fibres during cell division. In plants and animals, these regions often form megabase‐scale loci of tandemly repeated DNA sequences, which have presented a challenge to genomic studies even in model species. The functional designation of centromeres is determined epigenetically by the incorporation of a centromere‐specific variant of histone H3. Recent developments in long‐read sequencing technology have allowed the assembly of these regions for the first time and have prompted a reassessment of fidelity of centromere function and the evolutionary dynamics of these regions. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
45. A million-cow genome-wide association study of productive life in U.S. Holstein cows.
- Author
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Liang, Zuoxiang, Prakapenka, Dzianis, Zaabza, Hafedh B., VanRaden, Paul M., Van Tassell, Curtis P., and Da, Yang
- Subjects
GENOME-wide association studies ,HOLSTEIN-Friesian cattle ,LEAST squares ,CHROMOSOMES ,MILK yield - Abstract
Background: Productive life (PL) of a cow is the time the cow remains in the milking herd from first calving to exit from the herd due to culling or death and is an important economic trait in U.S. Holstein cattle. The large samples of Holstein genomic evaluation data that have become available recently provided unprecedented statistical power to identify genetic factors affecting PL in Holstein cows using the approach of genome-wide association study (GWAS). Methods: The GWAS analysis used 1,103,641 Holstein cows with phenotypic observations on PL and genotypes of 75,282 single nucleotide polymorphism (SNP) markers. The statistical tests and estimation of SNP additive and dominance effects used the approximate generalized least squares method implemented by the EPISNPmpi computer program. Results: The GWAS detected 5390 significant additive effects of PL distributed over all 29 autosomes and the X–Y nonrecombining region of the X chromosome (Chr31). Two chromosome regions had the most significant and largest cluster of additive effects, the SLC4A4-GC-NPFFR2 (SGN) region of Chr06 with pleiotropic effects for PL, fertility, somatic cell score and milk yield; and the 32–52 Mb region of Chr10 with peak effects for PL in or near RASGRP1 with many important immunity functions. The dominance tests detected 38 significant dominance effects including 12 dominance effects with sharply negative homozygous recessive genotypes on Chr18, Chr05, Chr23 and Chr24. Conclusions: The GWAS results showed that highly significant genetic effects for PL were in chromosome regions known to have highly significant effects for fertility and health and a chromosome region with multiple genes with reproductive and immunity functions. SNPs with rare but sharply negative homozygous recessive genotypes for PL existed and should be used for eliminating heifers carrying those homozygous recessive genotypes. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
46. Potential genetic gains from targeted recombination versus recurrent selection for maize yield and moisture.
- Author
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Cameron, John N. and Bernardo, Rex
- Subjects
- *
SINGLE nucleotide polymorphisms , *CHROMOSOME segregation , *HAPLOIDY , *CHROMOSOMES , *LOCUS (Genetics) - Abstract
Recurrent selection is a proven method and targeted recombination is a potential method for achieving genetic gain. Our objectives were to determine the (1) equivalency in predicted gains between cycles of recurrent selection and targeted recombination in maize (
Zea mays L.), (2) correspondence between targeted‐recombination positions when gains from nontargeted chromosomes are considered versus ignored, and (3) trade‐offs in predicted gains for yield and moisture in targeted recombination. From genomewide marker effects for 2911 single nucleotide polymorphism loci in 270 biparental populations, we obtained predicted gains from fixed numbers of targeted recombinations and compared these gains to the genotypic‐value distributions of doubled haploids obtained from different cycles of simulated recurrent selection. On average, it took three cycles of single‐trait recurrent selection to obtain at least a few doubled haploid lines with the same performance achieved with nine or 10 targeted recombinations in Cycle 0. Results indicated that when targeting recombinations across the genome, gains from segregation on nontargeted chromosomes do not need to be considered. Predicted increases in yield were associated with increases in moisture. Given the logistics of pyramiding targeted recombinations and the lack of mature technologies for routine targeted recombination, recurrent selection is currently the preferred approach for accumulating favorable recombinations. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
47. EYA-1 is required for genomic integrity independent of H2AX signalling in Caenorhabditis elegans.
- Author
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Tatnell, Hannah R., Novakovic, Stevan, Boag, Peter R., and Davis, Gregory M.
- Abstract
Background: Resolving genomic insults is essential for the survival of any species. In the case of eukaryotes, several pathways comprise the DNA damage repair network, and many components have high evolutionary conservation. These pathways ensure that DNA damage is resolved which prevents disease associated mutations from occurring in a de novo manner. In this study, we investigated the role of the Eyes Absent (EYA) homologue in Caenorhabditis elegans and its role in DNA damage repair. Current understanding of mammalian EYA1 suggests that EYA1 is recruited in response to H2AX signalling to dsDNA breaks. C. elegans do not possess a H2AX homologue, although they do possess homologues of the core DNA damage repair proteins. Due to this, we aimed to determine if eya-1 contributes to DNA damage repair independent of H2AX. Methods and results: We used a putative null mutant for eya-1 in C. elegans and observed that absence of eya-1 results in abnormal chromosome morphology in anaphase embryos, including chromosomal bridges, missegregated chromosomes, and embryos with abnormal nuclei. Additionally, inducing different types of genomic insults, we show that eya-1 mutants are highly sensitive to induction of DNA damage, yet show little change to induced DNA replication stress and display a mortal germline resulting in sterility over successive generations. Conclusions: Collectively, this study suggests that the EYA family of proteins may have a greater involvement in maintaining genomic integrity than previously thought and unveils novel roles of EYA associated DNA damage repair. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
48. Involvement of extracellular vesicle microRNA clusters in developing healthy and Rett syndrome brain organoids.
- Author
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Bahram Sangani, Nasim, Koetsier, Jarno, Gomes, Ana Rita, Diogo, Maria Margarida, Fernandes, Tiago G., Bouwman, Freek G., Mariman, Edwin C. M., Ghazvini, Mehrnaz, Gribnau, Joost, Curfs, Leopold M. G., Reutelingsperger, Chris P., and Eijssen, Lars M. T.
- Subjects
- *
GENE expression , *RETT syndrome , *EXTRACELLULAR vesicles , *NEUROLOGICAL disorders , *CHROMOSOMES - Abstract
Rett syndrome (RTT) is a neurodevelopmental disorder caused by de novo mutations in the MECP2 gene. Although miRNAs in extracellular vesicles (EVs) have been suggested to play an essential role in several neurological conditions, no prior study has utilized brain organoids to profile EV-derived miRNAs during normal and RTT-affected neuronal development. Here we report the spatiotemporal expression pattern of EV-derived miRNAs in region-specific forebrain organoids generated from female hiPSCs with a MeCP2:R255X mutation and the corresponding isogenic control. EV miRNA and protein expression profiles were characterized at day 0, day 13, day 40, and day 75. Several members of the hsa-miR-302/367 cluster were identified as having a time-dependent expression profile with RTT-specific alterations at the latest developmental stage. Moreover, the miRNA species of the chromosome 14 miRNA cluster (C14MC) exhibited strong upregulation in RTT forebrain organoids irrespective of their spatiotemporal location. Together, our results suggest essential roles of the C14MC and hsa-miR-302/367 clusters in EVs during normal and RTT-associated neurodevelopment, displaying promising prospects as biomarkers for monitoring RTT progression. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
49. CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC.
- Author
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Li, Qi, Chen, Qingzhou, Zheng, Tao, Wang, Fulin, Teng, Junlin, Zhou, Haining, and Chen, Jianguo
- Subjects
- *
CHROMOSOME segregation , *CELL cycle proteins , *KINETOCHORE , *ANAPHASE , *CHROMOSOMES - Abstract
The spindle assembly checkpoint (SAC) ensures chromosome segregation fidelity by manipulating unattached kinetochore‐dependent assembly of the mitotic checkpoint complex (MCC). The MCC binds to and inhibits the anaphase promoting complex/cyclosome (APC/C) to postpone mitotic exit. However, the mechanism by which unattached kinetochores mediate MCC formation is not yet fully understood. Here, it is shown that CCDC68 is an outer kinetochore protein that preferentially localizes to unattached kinetochores. Furthermore, CCDC68 interacts with the SAC factor CDC20 to inhibit its autoubiquitination and MCC disassembly. Therefore, CCDC68 restrains APC/C activation to ensure a robust SAC and allow sufficient time for chromosome alignment, thus ensuring chromosomal stability. Hence, the study reveals that CCDC68 is required for CDC20‐dependent MCC stabilization to maintain mitotic checkpoint activation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
50. Nearby and non-nested genes in the human genome have more similar genotype tissue expression.
- Author
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Dong, Jiahong, Brown, Stephen, and Truong, Kevin
- Subjects
- *
GENE expression , *HUMAN genome , *CHROMOSOMES , *NON-coding RNA , *PROMOTERS (Genetics) - Abstract
Neighboring genes within a shared promoter arrangement (i.e. opposite direction with the neighboring ends as the transcriptional start sites) are expected to have a high similarity in genotype tissue expression due to the potential overlap in the promoter region. This raises the question of whether similarity in expression profiles depends on orientation of the neighboring genes and whether there exist thresholds of locality where the similarity diminishes. Thus, in this work, we compared genotype tissue expression profiles at different genomic orientations and localities. Interestingly, there exist gene pairs in the human genome with very high or low expression similarity. Shorter chromosomes tend to have more similarly expressed genes. Also, a cluster of 3 adjacent genes within the average range of 20 to 60 kilobase pairs can have very similar expression profiles regardless of their orientations. However, when genes are nested and in opposite orientations, a lower than expected similarity was observed. Lastly, in cases where genotype tissue expression data does not exist or have low read counts (e.g. non-coding RNA), our identified influencing range can be a first estimate of the genotype tissue expression. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
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