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2024 SEP 7 (NewsRx) -- By a News Reporter-Staff News Editor at Obesity, Fitness & Wellness Week -- Investigators publish new report on Obesity, Fitness and Wellness. According to news [...]

2024 JUN 29 (NewsRx) -- By a News Reporter-Staff News Editor at Obesity, Fitness & Wellness Week -- New research on Cancer Research is the subject of a report. According [...]

2024 JAN 13 (NewsRx) -- By a News Reporter-Staff News Editor at Obesity, Fitness & Wellness Week -- Investigators discuss new findings in Biotechnology - Gene Therapy. According to news [...]

Hydrophobic interaction chromatography (HIC) is one of the historical analytical methods applied for the separation and purification of proteins. In the first part of this series, the elution and separation mechanisms were discussed. In this second part, some practical considerations such as method development, selection of the phase system, combined salt systems, and possibilities to combine HIC with other chromatographic modes are explained. Applications are critically discussed and the hyphenation to mass spectrometry (MS) briefly tackled., Historically, a kosmotropic salt--typically 1.5-2 M ammonium-sulfate--has been used as a mobile phase additive in hydrophobic interaction chromatography (HIC) (as well as 50-100 mM phosphate buffer). However, various salts can [...]

2022 SEP 3 (NewsRx) -- By a News Reporter-Staff News Editor at Obesity, Fitness & Wellness Week -- Investigators discuss new findings in Chemistry - Analytical Chemistry. According to news [...]

In clinical and pharmaceutical research, the application of reliable, reproducible, and the best-available analytical methods and techniques are necessary because the quality of analysis may have a crucial effect on the health and life of patients. The role of inorganic and organic ions, as well as ionizable substances, is very important for health. A very useful instrumental method for this range is ion chromatography (IC) and related techniques. Various ion chromatography methods are used in clinical and pharmaceutical research mainly to determine inorganic anions and cations, metals and metalloids, as well as selected organic compounds. This article presents possible uses of ion chromatography and related techniques combined with various detection methods for clinical and pharmaceutical analysis of common inorganic and organic anions and cations. An overview of achievements in this area from the past 10 years is presented and the most important trends and development perspectives for ion chromatography are described., Elements and chemical substances, particularly those that may be analyzed using ion chromatography (IC) and related techniques, play an important role in regular human health and life quality. This mainly [...]

In clinical and pharmaceutical research, the application of reliable, reproducible, and the best-available analytical methods and techniques are necessary because the quality of analysis may have a crucial effect on the health and life of patients. The role of inorganic and organic ions, as well as ionizable substances, is very important for health. A very useful instrumental method for this range is ion chromatography (IC) and related techniques. Various ion chromatography methods are used in clinical and pharmaceutical research mainly to determine inorganic anions and cations, metals and metalloids, as well as selected organic compounds. This article presents possible uses of ion chromatography and related techniques combined with various detection methods for clinical and pharmaceutical analysis of common inorganic and organic anions and cations. An overview of achievements in this area from the past 10 years is presented and the most important trends and development perspectives for ion chromatography are described., Elements and chemical substances, particularly those that may be analyzed using ion chromatography (IC) and related techniques, play an important role in regular human health and life quality. This mainly [...]

2022 MAY 7 (NewsRx) -- By a News Reporter-Staff News Editor at Obesity, Fitness & Wellness Week -- A new study on Obesity, Fitness and Wellness is now available. According [...]

Monoclonal antibodies are becoming a core aspect of the pharmaceutical industry. Together with a huge therapeutic potential, these molecules come with a structural complexity that drives state-of-the-art chromatography and mass spectrometry (MS) to its limits. This article discusses the use of micro-pillar array columns in combination with mass spectrometry for peptide mapping of monoclonal antibodies (mAbs) and antibody--drug conjugates (ADCs). Micro-pillar array columns are produced by a lithographic etching process creating a perfectly ordered separation bed on a silicon chip. As a result of the order existing in these columns, peak dispersion is minimized and highly efficient peptide maps are generated, providing enormous structural detail. Using examples from the author's laboratory, the performance of these columns is illustrated., The discovery of monoclonal antibodies (mAbs) in the 1970s and the realization of their therapeutic potential in the following decades has been a key milestone in medicine (1-3). Today, over [...]

2021 JAN 9 (NewsRx) -- By a News Reporter-Staff News Editor at Obesity, Fitness & Wellness Week -- Current study results on Technology - Purification Technology have been published. According [...]

These are exciting times to be involved in monoclonal antibody (mAb) and biopharmaceutical analysis. Advances in instrumentation, column technology, and reagents are providing analysts with a new set of tools [...]

Glycosylation of monoclonal antibody (mAb) therapeutics is widely recognized by the regulators and the industry as a critical quality attribute (CQA). Hence, it is necessary that glycosylation is measured and [...]

2019 NOV 16 (NewsRx) -- By a News Reporter-Staff News Editor at Obesity, Fitness & Wellness Week -- New research on Biotechnology - Gene Therapy is the subject of a [...]

2019 JUN 29 (NewsRx) -- By a News Reporter-Staff News Editor at Obesity, Fitness & Wellness Week -- Current study results on RNA Viruses - Bluetongue Virus have been published. [...]

2018 MAY 26 (NewsRx) -- By a News Reporter-Staff News Editor at Obesity, Fitness & Wellness Week -- Investigators publish new report on Drugs and Therapies - Cancer Therapy. According [...]

Sartobind STIC (salt-tolerant interaction chromatography, Sartorius Stedim Biotech, Goettingen, Germany), a salt-tolerant anion-exchange membrane adsorber, has demonstrated proof-of-concept in removing residual host-cell impurities from high-salt, packed-bed affinity chromatography eluate. Although [...]

OBJECTIVE--Dietary factors influence diabetes development in the NOD mouse. Diet affects the composition of microbiota in the distal intestine, which may subsequently influence intestinal immune homeostasis. However, the specific effects of anti-diabetogenic diets on gut immunity and the explicit associations between intestinal immune disruption and type 1 diabetes onset remain unclear. RESEARCH DESIGN AND METHODS--Gut microbiota of NOD mice fed a conventional diet or ProSobee formula were compared using gas chromatography. Colonic lamina propria immune cells were characterized in terms of activation markers, cytokine mRNA and Th17 and [Foxp3.sup.+] T-cell numbers, using real-time PCR and flow cytometry. Activation of diabetogenic CD4 T-cells by purified B-cells was assessed in both groups. Immune tolerance to autologous commensal bacteria was evaluated in vitro using thymidine-incorporation tests. RESULTS--Young NOD mice showed a disturbed tolerance to autologous commensal bacteria. Increased numbers of activated CD4 T-cells and ([CD11b.sup.+][CD11c.sup.+]) dendritic cells and elevated levels of Th17 cells and IL23 mRNA were moreover observed in colon lamina propria. These phenomena were abolished when mice were fed an antidiabetogenic diet. The antidiabetogenic diet also altered the expression levels of costimulatory molecules and the capacity of peritoneal B-cells to induce insulin-specific CD4 T-cell proliferation. CONCLUSIONS--Young NOD mice show signs of subclinical colitis, but the symptoms are alleviated by a diet change to an antidiabetogenic diet. Disrupted immune tolerance in the distal intestine may influence peritoneal cell pools and B-cell-mediated activation of diabetogenic T-cells. Diabetes 59:2237-2246, 2010, Dietary and microbial factors may be partly responsible for the increase in type 1 diabetes incidence. The intestinal mucosa is constantly exposed to these factors, and it is therefore important [...]

ABSTRACT Challenges in the purification of monoclonal antibodies (MAbs) include reducing production cost, developing robust processes for both product purity and viral clearance, and integrating upstream and downstream processes. Increases [...]

2017 MAR 4 (NewsRx) -- By a News Reporter-Staff News Editor at Obesity, Fitness & Wellness Week -- Data detailed on Chemistry - Analytical Chemistry have been presented. According to [...]

ABSTRACT Membrane technology is cited as one of the most exciting current market trends in the biopharmaceutical industry. This article examines the principles of membrane chromatography and expands on a [...]

2016 AUG 20 (NewsRx) -- By a News Reporter-Staff News Editor at Obesity, Fitness & Wellness Week -- Data detailed on Drugs and Therapies have been presented. According to news [...]

2015 AUG 15 (NewsRx) -- By a News Reporter-Staff News Editor at Obesity, Fitness & Wellness Week -- Investigators publish new report on Oncology. According to news originating from Vitoria, [...]

By a News Reporter-Staff News Editor at Obesity, Fitness & Wellness Week -- Investigators discuss new findings in Pesticides. According to news reporting originating from Bialystok, Poland, by NewsRx editors, [...]